WO2018137335A1

WO2018137335A1 - Blood detection method and device

Info

Publication number: WO2018137335A1
Application number: PCT/CN2017/097791
Authority: WO
Inventors: 白新梅; 张勇; 邵汉荣
Original assignee: 深圳开立生物医疗科技股份有限公司
Priority date: 2017-01-24
Filing date: 2017-08-17
Publication date: 2018-08-02
Also published as: CN106771107A

Abstract

A blood detection method and a blood detection device using the blood detection method. The blood detection method comprises: controlling a sampling needle to collect a primary blood sample; controlling the sampling needle to distribute the primary blood sample into a routine blood test module; controlling a sampling needle to collect a secondary blood sample from the routine blood test module; controlling the sampling needle to distribute the secondary blood sample into a CRP test module; performing a routine blood test of the primary blood sample in the routine blood test module, and performing a CRP test of the secondary blood sample in the CRP test module. During the detection, the secondary blood sample required for the CRP test is collected from the primary blood sample in the routine blood test module. Increasing detection items will not required an increased blood usage amount, and the total consumption of blood is always equal to the amount of the collected primary blood sample; in addition, no isolated sample is set, decreasing the total consumption of the blood sample and avoiding waste.

Description

Blood detecting method and device

The present application claims priority to Chinese Patent Application, filed on Jan. 24, 2017, filed Jan. .

Technical field

The invention relates to the field of blood analysis, in particular to a blood detecting method, and to a blood detecting device.

Background technique

In the field of blood analysis, blood routine testing and detection of C-reactive protein (CRP) are the most common applications. In order to achieve rapid detection of blood routine and CRP parameters to meet clinical testing needs, it is necessary to integrate a blood routine detection module and a CRP detection module into the blood analyzer.

Due to the principle of blood routine and CRP detection, the reagents used are different, so at least two separate channels are required for measurement, and independent sample collection and distribution are required for these channels. The existing method is to take an appropriate amount of blood at a time, and then realize sample distribution to different detection channels by quantitative control. That is, firstly, a certain amount of blood satisfying the measurement requirement is sucked through the sampling needle, and blood is sequentially distributed to each measurement module, but the blood of the isolation section needs to be set before the blood is distributed to each measurement module, and the blood of the isolation section is discarded.

In this way, because the CRP channel and the blood channel are independently assigned blood samples, the total blood volume is increased, or the corresponding blood routine module sample is reduced, thereby weakening the competitiveness of the product in blood sample consumption, or affecting blood routine. The module detects the accuracy level of the project; at the same time, the isolated segment samples are set in each allocation segment, which invisibly increases the overall sample consumption and wastes.

Therefore, how to provide a blood detecting method for reducing the total consumption of blood samples and improving the accuracy of detection is a technical problem that a person skilled in the art needs to solve at present.

Summary of the invention

It is an object of the present invention to provide a blood test method capable of reducing the total consumption of blood samples And to improve the detection accuracy, another object of the present invention is to provide a blood detecting device capable of reducing the total consumption of blood samples and improving the detection accuracy.

In order to solve the above technical problem, the present invention provides a blood detecting method, comprising:

Control the sampling needle to collect the initial blood sample;

Controlling the sampling needle to dispense the initial blood sample to a blood routine measurement module;

Controlling the sampling needle to collect a secondary blood sample from the blood routine measurement module;

Controlling the sampling needle to dispense the secondary blood sample to the CRP measurement module;

Performing blood routine detection on the initial blood sample in the blood routine measurement module, and performing CRP detection on the secondary blood sample in the CRP measurement module.

Preferably, controlling the sampling needle to dispense the initial blood sample to the blood routine measurement module further comprises diluting the initial blood sample, the second blood sample being specifically the partially diluted blood sample.

Preferably, the controlling the sampling needle after collecting the initial blood sample further comprises discarding the first segment of the initial blood sample of the preset dose.

Preferably, the controlling the sampling needle to allocate the initial blood sample to a blood routine measurement module, in particular, controlling the sampling needle to sequentially distribute the initial blood sample to a plurality of blood routine measurement modules;

The controlling the sampling needle collects a second blood sample from the blood routine measuring module, specifically, controlling the sampling needle to collect the second blood sample into the first type of blood routine measuring module.

Preferably, the controlling the sampling needle to distribute the second blood sample to the CRP measurement module further comprises controlling the sampling needle to sequentially distribute the second blood sample to a plurality of second type blood routine measurement modules; The second blood sample in the second type of blood routine measurement module is subjected to blood routine examination.

Preferably, the controlling the sampling needle to sequentially distribute the second blood sample to the plurality of second type blood routine measurement modules further comprises cleaning the sampling needle.

Preferably, the total dose of the initial blood sample is the predetermined dose of the first segment of the initial blood sample discarded and the divided dose of the initial blood sample allocated in each of the blood routine measurement modules with;

The total dose of the secondary blood sample is a divided dose of the secondary blood sample distributed in the CRP measuring module and a divided dose of the secondary blood sample distributed in each of the two types of blood routine measuring modules with.

The invention also provides a blood testing device comprising:

a sampling needle for collecting blood samples;

Blood routine measurement module for blood routine testing;

CRP measurement module for performing CRP detection;

a controller, configured to control the sampling needle to collect the initial blood sample, and then allocate the initial blood sample to the blood routine measurement module, and control to control the sampling needle to be collected by the blood routine measurement module after collecting the second blood sample The secondary blood sample is dispensed to the CRP measurement module.

Preferably, the blood routine measurement module includes a plurality of blood routine measurement modules for detecting the initial blood samples and a plurality of blood routine measurement modules for detecting the secondary blood samples.

The invention provides a blood detecting method, comprising: controlling a sampling needle to collect an initial blood sample; controlling a sampling needle to allocate an initial blood sample to a blood routine measuring module; controlling the sampling needle to collect a second blood sample from a blood routine measuring module; and controlling the sampling needle A secondary blood sample is dispensed to the CRP measurement module; a blood routine test is performed on the initial blood sample in the blood routine measurement module, and a CRP test is performed on the secondary blood sample in the CRP measurement module. During the detection process, the secondary blood sample required for CRP detection is collected from the blood routine measurement module. Increasing the test item does not increase the blood volume. The total blood volume is always the dose of the initial blood sample collected. Do not set up isolated segment samples to reduce the total consumption of blood samples and avoid waste.

The present invention also provides a blood detecting device using the above blood detecting method. Since the blood detecting method has the above technical effects, the blood detecting device should have the same technical effect, and will not be described in detail herein.

DRAWINGS

1 is a flow chart of a specific embodiment of a blood detecting method provided by the present invention;

2 is a specific embodiment of a blood detecting method provided by the present invention in a dispensing process Schematic diagram of the state changes of blood samples inside the sampling needle at different stages.

detailed description

The core of the present invention is to provide a blood detecting method capable of reducing the total consumption of blood samples and improving the detection accuracy. Another core of the present invention is to provide a blood detecting device capable of reducing the total consumption of blood samples and improving the detection accuracy. Sex.

The present invention will be further described in detail below in conjunction with the drawings and embodiments.

Please refer to FIG. 1. FIG. 1 is a flow chart of a specific embodiment of a blood detecting method according to the present invention.

A blood testing method provided by a specific embodiment of the present invention includes the steps of:

The sampling needle is used to collect the initial blood sample. After the collection is completed, the first initial blood sample of the preset dose needs to be discarded to prevent contamination and improve the detection accuracy.

The sampling needle is controlled to dispense an initial blood sample to the blood routine measurement module.

The control sampling needle collects a secondary blood sample from the blood routine measurement module, that is, a part of the initial blood sample distributed to the blood routine measurement module before the collection as a secondary blood sample.

The sampling needle is controlled to dispense a secondary blood sample to the CRP measurement module.

Perform routine blood test on the initial blood sample in the blood routine measurement module, perform CRP test on the secondary blood sample in the CRP measurement module, complete the corresponding detection item, and perform two tests simultaneously after the sample distribution is completed, or It is within the scope of the present invention to directly perform the detection separately after the completion of the allocation within the modules.

During the detection process, the secondary blood sample required for CRP detection is collected by the blood routine measurement module. Increasing the test item does not increase the blood volume. The total blood volume is always the dose of the initial blood sample collected. Do not set up isolated segment samples to reduce the total consumption of blood samples and avoid waste.

In the blood detecting method provided by the embodiment of the present invention, after the sampling needle is allocated to the blood routine measuring module, the initial blood sample is further diluted to include the diluted initial blood sample, that is, the second blood sample is the partially diluted initial blood sample. The initial blood sample is distributed to the blood routine measurement module for pre-dilution or hemolysis staining; then the secondary blood sample added to the CRP measurement module is Sampled from a pre-diluted reaction sample. That is to say, the sample added to the CRP measurement module in the present scheme is a pre-diluted sample obtained from the blood routine measurement module, which ensures the sample dose required during the detection process and improves the detection accuracy.

The blood routine measurement module may include a plurality of blood routine measurement modules to perform different detection items, so the control sampling needle is assigned to the blood routine measurement module to allocate an initial blood sample, specifically, the control sampling needle is sequentially distributed to a plurality of blood routine measurement modules. After the initial blood sample is allocated to the first type of blood routine measurement module, the sampling needle is moved to the next type of blood routine measurement module for sequential distribution until the initial blood sample distribution in the sampling needle is completed; The second blood sample is collected in the blood routine measurement module, and the second blood sample is collected from the first blood routine measurement module for controlling the sampling needle.

It is also possible to add a new blood routine test item, and the use of the secondary blood sample still does not increase the total blood consumption. Therefore, after the control sample needle is assigned to the CRP measurement module to the second blood sample, the control sample needle is sequentially controlled to a plurality of types. The blood routine measurement module allocates a secondary blood sample; and performs a blood routine examination on the secondary blood sample in the second type blood routine measurement module. After the sample is dispensed, the sample needle needs to be cleaned.

Based on the blood test method provided by each of the above embodiments, the total dose of the initial blood sample is the predetermined dose of the discarded first blood sample and the initial blood sample allocated in each type of blood routine measurement module. The sum of the doses, that is, the discarded first sample, the remaining initial blood samples are all distributed to the measurement module, and no isolation segment is provided to avoid waste. Similarly, the total dose of the secondary blood sample is the sum of the divided dose of the secondary blood sample distributed in the CRP measuring module and the divided dose of the secondary blood sample distributed in each of the two types of blood conventional measuring modules, that is, the second collected Blood samples are also all dispensed into the corresponding measurement module, with no discarded samples.

A specific embodiment of the present invention also provides a blood detecting apparatus using the above blood detecting method, comprising a sampling needle for collecting a blood sample, a blood conventional measuring module for performing blood routine detection, and a CRP measuring module for performing CRP detection. And a controller, wherein the controller is configured to control the sampling needle to collect the initial blood sample, and then allocate the initial blood sample to the blood routine measuring module, and control the sampling needle to be collected from the blood routine measuring module to the CRP measuring module. Secondary blood samples. The blood routine measurement module includes a plurality of blood routine measurement modules for detecting initial blood samples and a plurality of blood routine measurement modules for detecting secondary blood samples. Piece.

Specifically, two types of blood routine measurement modules, one CRP measurement module, and one second type blood routine measurement module may be set, and the number of each module may be adjusted according to the situation, which is within the protection scope of the present invention. Please refer to FIG. 2. FIG. 2 is a schematic diagram showing the state change of the blood sample inside the sampling needle at different stages in the dispensing process according to a specific embodiment of the blood detecting method provided by the present invention.

1 is the first sample to be discarded, 2 is the sample allocated in the first blood routine measurement module, 3 is the sample assigned in the subsequent blood routine measurement module, and 4 is the sample allocated in the CRP measurement module. A sample assigned to a blood test module of the second type. 4 and 5 are pre-diluted samples obtained from the first class of blood routine measurement modules. Specific steps are as follows:

After the sampling needle collects a certain amount of blood from the tube in which the blood sample is stored, as shown by A, the first sample of the preset dose is discarded, and the sample state inside the sampling needle is as shown in B.

The sampling needle distributes the sample to the first type of blood routine measurement module. After completion, the sample state in the sampling needle is as shown in C.

The sampling needle distributes the sample to the subsequent blood routine measurement module. The sample state in the sampling needle is as shown in D. At this time, the collected blood sample in the sampling needle has been allocated.

The sampling needle collects a certain amount of diluted blood sample into the first blood type conventional measurement module. After completion, the sample state in the sampling needle is as shown in E.

The sampling needle distributes the sample to the CRP measuring module. After completion, the sample state in the sampling needle is as shown in F.

The sampling needle distributes the sample to the second type blood routine measurement module. After completion, the sample state in the sampling needle is as shown in G; at this time, the pre-dilution blood sample collected from the first type of blood routine measurement module in the sampling needle has been dispensed, and the cleaning is performed. Sampling needle.

The blood detection method provided by the present invention has been described in detail above. The principles and embodiments of the present invention have been described herein with reference to specific examples, and the description of the above embodiments is only to assist in understanding the method of the present invention and its core idea. It should be noted that those skilled in the art can make various modifications and changes to the present invention without departing from the spirit and scope of the invention.

Claims

A blood detecting method, comprising:

Control the sampling needle to collect the initial blood sample;

Controlling the sampling needle to dispense the initial blood sample to a blood routine measurement module;

Controlling the sampling needle to collect a secondary blood sample from the blood routine measurement module;

Controlling the sampling needle to dispense the secondary blood sample to the CRP measurement module;

Performing blood routine detection on the initial blood sample in the blood routine measurement module, and performing CRP detection on the secondary blood sample in the CRP measurement module.
The blood detecting method according to claim 1, wherein controlling the sampling needle to distribute the initial blood sample to the blood routine measuring module further comprises diluting the initial blood sample, the secondary blood sample being specifically a part The initial blood sample after dilution.
The blood detecting method according to claim 2, wherein the controlling the sampling needle to collect the initial blood sample further comprises discarding the first portion of the initial blood sample of the predetermined dose.
The blood detecting method according to claim 3, wherein the controlling the sampling needle to distribute the initial blood sample to the blood routine measuring module is specifically controlling the sampling needle to sequentially perform a plurality of blood routine measuring modules. Allocating the initial blood sample;

The controlling the sampling needle collects a second blood sample from the blood routine measuring module, specifically, controlling the sampling needle to collect the second blood sample into the first type of blood routine measuring module.
The blood detecting method according to claim 4, wherein the controlling the sampling needle to the CRP measuring module to distribute the second blood sample further comprises controlling the sampling needle to sequentially perform measurement to a plurality of types of blood The module assigns the secondary blood sample; performs a blood routine examination on the secondary blood sample in the second type of blood routine measurement module.
The blood detecting method according to claim 5, wherein the controlling the sampling needle to sequentially dispense the second blood sample to the plurality of blood type conventional measuring modules further comprises washing the sampling needle.
The blood detecting method according to any one of claims 1 to 6, wherein the total dose of the initial blood sample is the predetermined dose of the first segment of the initial blood sample discarded and each of the ones a sub-dose of the initial blood sample dispensed within a blood-like conventional measurement module with;

The total dose of the secondary blood sample is a divided dose of the secondary blood sample distributed in the CRP measuring module and a divided dose of the secondary blood sample distributed in each of the two types of blood routine measuring modules with.
A blood testing device, comprising:

a sampling needle for collecting blood samples;

Blood routine measurement module for blood routine testing;

CRP measurement module for performing CRP detection;

a controller, configured to control the sampling needle to collect the initial blood sample, and then allocate the initial blood sample to the blood routine measurement module, and control to control the sampling needle to be collected by the blood routine measurement module after collecting the second blood sample The secondary blood sample is dispensed to the CRP measurement module.
The blood testing apparatus according to claim 8, wherein said blood routine measuring module comprises a plurality of blood routine measuring modules for detecting said initial blood sample and a plurality of blood detecting electrodes The second type of blood routine measurement module of the sample.