CN103575634B - A kind of streaming instrument and its liquid channel system, method - Google Patents
A kind of streaming instrument and its liquid channel system, method Download PDFInfo
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Abstract
The invention discloses a kind of streaming instrument and its liquid channel systems, method, liquid channel system includes flow chamber, sample provides device, sheath fluid provides device, waste liquid absorption plant and N number of proportioning device, the pipeline of each proportioning device is respectively communicated with flow chamber and sample provides device, the N is greater than or equal to 2, each proportioning device provides device from sample according to setting sequential and draws quantitative sample liquid, and sample is transported to flow chamber by least one proportioning device according to the scheduled sample amount that pushes away in each period, therefore constantly sample can be sent into flow chamber, it is preset due to pushing away sample amount, it therefore can be with the volume of accurate quantification tested sample, realize accurate metering, and avoid the interruption in test process, realize the test constantly to sample, shorten the pattern detection time.
Description
Technical field
The present invention relates to particle analysis field more particularly to a kind of streaming instruments and its liquid channel system.
Background technology
Analysis and statistics of the streaming instrument commonly used in fine particle, in medical field, it is thin that streaming instrument is also referred to as streaming
Born of the same parents' analyzer obtains the acquisition for being collected cell sum, but some for being classified to cell and being counted, therefore usually required that
In the case of user need to obtain the concentration information for being collected cell in sample, therefore it is required that streaming instrument is right in use
Part sample needs to carry out absolute counting, to obtain the concentration information for being collected cell, and remaining most of sample is needed
It is non-absolute counting (i.e. common survey), to meet the demand of the acquisition sum to being collected cell.
The absolute counting for being collected the requirement of cell collection sum to make streaming instrument that can meet and realizing to sample
Function, there are two types of schemes for streaming instrument at present：One kind is absolute volume metered scheme；Another kind is the standard that known concentration is added
Particle measurement scheme.
For absolute volume metered scheme, mainly pass through the quantitative method of the device component of streaming instrument, such as quantitative
Pump etc., clearly knows the volume of tested sample, then by the volume of the number for being collected cell divided by tested sample that measure, i.e.,
The concentration information for being collected cell can be obtained, achievees the purpose that absolute counting.
For another scheme, there is no quantitative device component in streaming instrument, when user needs absolutely to count sample
When number, i.e., be added the standard particle of known concentration into sample, this scheme need standard particle by known concentration with
Know that the sample mixing of volume, the standard particle concentration in sample mixed liquor can be calculated.Sample mixed liquor is passed through into streaming
Instrument measures, and can measure standard particle number and the number for being collected cell, according to standard particle number can inverse go out by
Volume is surveyed, then by the number for being collected cell measured divided by the tested volume of sample mixed liquor, you can obtain being received
Collect the concentration information of cell, reaches the purpose of absolute counting.This scheme is needed by known concentration normalized particle inverse sample body
Long-pending method needs user additionally accurately to allot the concentration of standard particle, more demanding to user, inconvenient to use, and increases
The use cost of user.And conventional absolute volume metering method needs to push away sample soon using constant displacement pump, due to quantitative every time
Volume be it is limited, when it is primary push away sample and cannot meet the needs when, constant displacement pump needs to push away sample again after inhaling sample again, wastes time,
Lead to occur measuring discontinuous problem in sample measurement, and there are sample waste rate height, measurement efficiencies for the measurement of fixed quantity cycle
Low disadvantage.
Invention content
A kind of streaming instrument of present invention offer and its liquid channel system, method, in the premise for realizing accurate quantification sample volume
Under, achieve the purpose that test constantly sample.
According to the first aspect of the invention, a kind of liquid channel system of streaming instrument is provided, including：
Flow chamber sweeps along sample flow to pass through, and provides the place being irradiated by light for sample flow for accommodating sheath fluid；
Sample provides device, for drawing sample from tested sample pipe；
Sheath fluid provides device, pipeline connection to flow chamber, for sheath fluid to be transported to flow chamber；
Waste liquid absorption plant, pipeline connection to flow chamber, for receiving the waste liquid flowed out from flow chamber；
The pipeline of N number of proportioning device, each proportioning device is respectively communicated with flow chamber and sample provides device, the N
More than or equal to 2, each proportioning device provides device from sample according to setting sequential and draws quantitative sample liquid, and in each period
Sample is transported to flow chamber by least one proportioning device according to the scheduled sample amount that pushes away.
According to the second aspect of the invention, a kind of streaming instrument, including liquid channel system, Systems for optical inspection and control are provided
Device, the liquid channel system are used to provide tested sample stream and collect the sample flow waste liquid after detection, and the Systems for optical inspection is used
It in the light beam of offer irradiation sample flow, and obtains sample and flows through the information after light beam irradiation, the liquid channel system includes：
Flow chamber sweeps along sample flow to pass through, and provides the place being irradiated by light for sample flow for accommodating sheath fluid；
Sample provides device, for drawing sample from tested sample pipe；
Sheath fluid provides device, pipeline connection to flow chamber, for sheath fluid to be transported to flow chamber；
Waste liquid absorption plant, pipeline connection to flow chamber, for receiving the waste liquid flowed out from flow chamber；
The pipeline of N number of proportioning device, each proportioning device is respectively communicated with flow chamber and sample provides device, the N
More than or equal to 2；
The controller is connect with proportioning device signal respectively, is controlled each proportioning device and is carried from sample according to setting sequential
Quantitative sample liquid is drawn for device, and sample is pushed away the conveying of sample amount by least one proportioning device according to scheduled in each period
To flow chamber.
According to the third aspect of the invention we, a kind of method carrying out particle analysis using streaming instrument, the streaming are provided
Instrument includes liquid channel system, Systems for optical inspection and controller, and the liquid channel system is examined for providing tested sample stream and collecting
Sample flow waste liquid after survey, the Systems for optical inspection are used to provide the light beam of irradiation sample flow, and obtain sample and flow through light beam
Information after irradiation, the liquid channel system include N number of proportioning device, and the N is greater than or equal to 2, the method includes：Control is every
A proportioning device provides device from sample according to setting sequential and draws quantitative sample liquid, and at least one is quantitative in each period
Sample is transported to flow chamber by device according to the scheduled sample amount that pushes away.
Liquid channel system provided by the present application, due to its proportioning device have it is multiple, be proportioning device joint inhale sample
Possibility is provided with sample is pushed away.
Streaming instrument provided by the present application and particle analysis method are combined by two or more proportioning devices
It inhales and sample and pushes away sample so that each period, at least one proportioning device carried out to flow chamber pushing away sample according to the scheduled sample amount that pushes away,
Therefore can constantly by sample be sent into flow chamber in, due to push away sample amount be it is preset, can be with accurate quantification detected sample
This volume realizes accurate metering, and avoids the interruption in test process, realizes the test constantly to sample, shortens
Pattern detection time.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of embodiment of the application；
Fig. 2 is the structural schematic diagram of the application another kind embodiment.
Specific implementation mode
Below by specific implementation mode combination attached drawing, invention is further described in detail.
The term used in the application is explained first.
Absolute counting：Refer to the detection carried out to obtain the concentration information for being collected cell, is needed in this detection
Obtain the sample volume of the number for being collected cell and detection.
It pushes away soon：It typically refers to quantitative portion's device quickly to shift sample in flow chamber onto, to wash away the liquid taken in the duct
Body, such as dilution or the sample that is diluted.Required sample size is pushed away soon is known as fast pushing volume.
Push away sample amount：It is the sample size that specified amount portion device is pushed to when pushing away sample in flow chamber.
In the embodiment of the present application, streaming instrument is combined using two or more proportioning devices pushes away sample, and it is each fixed to make
Measure device provides device absorption quantitative sample liquid according to setting sequential from sample, and at least one proportioning device of each period
Sample is transported to flow chamber according to the scheduled sample amount that pushes away, i.e., if by sample optical detection time be divided into it is several push away the sample period and
The sample period is inhaled, for only there are one the technical solution of proportioning device, control mode is that proportioning device is inhaling sample period absorption sample
This, inhales and pushes away sample to flow chamber pushing away the sample period after the completion of sample, pushes away to enter back into after the completion of sample and inhales the sample period and draw sample, and the application
The control mode of multiple proportioning devices is no matter which period to have proportioning device to push away sample according to scheduled in embodiment
Sample amount carries out pushing away sample to flow chamber, i.e., the sample volume for constantly having sample flow to flow through in flow chamber, and flowing through flow chamber is each
The secondary summation for pushing away sample amount.To while realizing accurate quantification sample volume, achieve the purpose that test constantly sample.The streaming
Instrument includes liquid channel system, Systems for optical inspection and controller, and liquid channel system is for providing tested sample stream and package sample flow
Sheath fluid and collect the waste liquid after detection, Systems for optical inspection is used to provide the light beam for irradiating sample flow, and obtains sample flow
Information after light beam irradiates, liquid channel system includes flow chamber, sample provides device, sheath fluid provides device, waste liquid absorption plant
With at least two proportioning devices.Flow chamber sweeps along sample flow to pass through for accommodating sheath fluid, and provides and be irradiated by light for sample flow
Place；Sample provides device for drawing sample from tested sample pipe；Sheath fluid provides the pipeline connection of device to flow chamber,
For sheath fluid to be transported to flow chamber；The pipeline connection of waste liquid absorption plant is flowed out for receiving from flow chamber to flow chamber
Waste liquid；The pipeline of N number of proportioning device is all respectively communicated with flow chamber and sample provides device, and N is greater than or equal to 2.Controller point
It is not connect with N number of proportioning device signal, signal connection includes that wired connection and wireless communication connect.Controller is each for controlling
Proportioning device provides device from sample according to setting sequential and draws quantitative sample liquid, and at least one is quantitatively filled in each period
It sets and sample is transported to flow chamber according to the scheduled sample amount that pushes away, such as in one embodiment, controller can control N number of quantitative
Device connects successively is transported to flow chamber by sample according to the scheduled sample amount that pushes away, i.e., after the completion of a proportioning device pushes away sample, tightly
Then it controls next proportioning device to carry out pushing away sample, cycle constantly has sample flow flow chamber successively, until detection terminates.Optics
Detecting system is generally arranged near flow chamber, on the one hand Systems for optical inspection forms light beam, and light beam irradiation is made to flow through flowing
Sample flow in room, on the other hand, Systems for optical inspection detection sample flow through the information after light beam irradiation, obtain the forward direction of sample
Signal, lateral signal and fluorescence signal, are then handled by back end signal, and final result is exported.
Due to being provided with multiple proportioning devices, multiple proportioning devices can inhale sample and push away sample in turn, i.e., each period has one
A proportioning device conveys sample to flow chamber, so follow-on test can be carried out to the sample flow in flow chamber, avoids because waiting for
Proportioning device draws sample again and test is caused to be interrupted, so the pattern detection time can be reduced.And due to flowing to flow chamber
Sample flow is not interrupted, so only needing to carry out once pushing away sample when detecting beginning soon, test is intermediate need not to push away sample soon,
Therefore reduce sample caused by pushing away soon to waste, improve sample utilization rate.
The mode that serial or parallel connection may be used in multiple proportioning devices is arranged between flow chamber and sample provide device
On pipeline.The flow direction of sample flow in order to control, when proportioning device is connected on the pipeline between flow chamber and sample offer device,
Sample flow control valve, controller control are provided between two proportioning devices and between proportioning device and sample offer device
Sample flow control valve opens or closes, and when controller, which controls some proportioning device, conveys sample to flow chamber, controls simultaneously
The proportioning device is opened to the sample flow control valve between flow chamber, is controlled the proportioning device and is provided on device path to sample
First sample flow control valve is closed, and so that sample flow is smoothly flowed to flow chamber to reach, is prevented the purpose of sample flow reverse flow,
Other proportioning devices that the proportioning device is provided to sample on device path are not interfered to draw sample again simultaneously.When proportioning device simultaneously
When being associated in flow chamber and sample and providing on pipeline between device, between proportioning device and flow chamber and proportioning device and
Sample flow control valve is arranged between providing device in sample, and sample flow control valve is controlled by controller, can open or close state
Between switch over.When controller, which controls some proportioning device, conveys sample to flow chamber, the proportioning device and flowing are controlled
Sample flow control valve between room is opened, while being controlled the sample flow control valve that the proportioning device and sample are provided between device and being closed
It closes, so that sample will not be reverse flowed to sample and provide device；When controller controls some proportioning device device suction is provided from sample
This when is sampled, the sample flow control valve controlled between the proportioning device and flow chamber is closed, while controlling the proportioning device and sample
Sample flow control valve between this offer device is opened, and so that sample flow is only provided device from sample and is flowed to proportioning device, prevents sample
This stream flows to flow chamber.
Constant displacement pump, syringe or the volume that sample is obtained using other schemes, such as sample for example can be used in proportioning device
Pipe level sensing scheme, sample tube weigh scheme, by incude sample tube capacitance variations convert volume change, by incude sample
This pipe electromagnetic change conversion volume change etc..
Proportioning device can be two, three or four, be illustrated so that proportioning device is two as an example below.
Embodiment one：
Referring to FIG. 1, waste liquid absorption plant includes the waste liquid pool 110 and first for accommodating the waste liquid flowed out from flow chamber
Control valve 109, the first control valve 109 are arranged on the pipeline between waste liquid pool 110 to flow chamber 108 exports, and it is controllable
It opens or closes and switches between state；It includes being controlled for accommodating the liquid storage tank 106 and second of sheath fluid that sheath fluid, which provides device,
Valve 107, the second control valve 107 are arranged on liquid storage tank 106 to the pipeline between 108 entrance of flow chamber, and controllable are opening
Or it is switched between closed state.Proportioning device includes the first proportioning device 103 and the second proportioning device 105, and sample provides
Device is sampling needle 112, and sampling needle 112 can be inserted into tested sample pipe 101 and draw sample.First proportioning device 103 and second
Proportioning device 105 is arranged in series on the pipeline between flow chamber 108 and sampling needle 112, the first proportioning device 103
For close to the proportioning device of sampling needle 112, the second proportioning device 105 is the proportioning device close to flow chamber 108, quantitative first
Pipeline between device 103 and sampling needle 112 is equipped with first sample flow control valve 102, and first sample flow control valve 102 is controllable
Switched between opening or closing state, on the pipeline between the first proportioning device 103 and the second proportioning device 105
Equipped with the second sample flow control valve 104, the second sample flow control valve 104 is controllable to be cut between opening or closing state
It changes.Systems for optical inspection 111 is generally arranged at the opposite both sides of flow chamber 108, is acted on through sample for emitting light beam and receiving
Optical signal afterwards.
Various control valves in above-described embodiment can select as needed, such as select solenoid valve, controller（Do not show in figure
Go out）It is connect respectively with each control valve and proportioning device, control the switching of solenoid valve and the suction sample of proportioning device and pushes away sample behaviour
Make.
When being measured to sample using the streaming instrument in the present embodiment, flow can be as follows：
1, the dilution taken in from sampling needle 112 to pipeline flow chamber 108 is arranged by inhaling sample and fast push operation
Go out, liquid dilution is diluted to avoid the sample of subsequent delivery.The first proportioning device 103 may be used to complete to inhale sample and push away soon,
The second proportioning device 105 may be used to complete to inhale sample and push away soon, the first proportioning device 103 and the second proportioning device can also be used
105 cooperations are completed to inhale sample and be pushed away soon.
2, two proportioning devices are controlled and sample is alternately transported to flow chamber according to the scheduled sample amount that pushes away.
It can be that the first proportioning device 103 pushes away sample first, can also be that the second proportioning device 105 pushes away sample first.
Since the first proportioning device 103 is connected in series with the second proportioning device 105 in flow chamber 108 and sampling
On pipeline between needle 112, therefore, the second proportioning device 105 can push away the mistake of sample in the first proportioning device 103 to flow chamber 108
It is transported in the sample in pipeline from the first proportioning device 103 in journey and carries out suction sample, and the suction sample of the second proportioning device 105 is
A certain amount of device 103 is pushed away before sample terminates and is completed, and the sample size that the first proportioning device 103 is drawn is equal to the first proportioning device 103
What the sample size conveyed to flow chamber 108 was drawn with the second proportioning device 105 from the sample flow that the first proportioning device 103 is released
The integral multiple of the sum of sample size, and the sample speed that pushes away of the first proportioning device pushes away the 2 of sample speed more than or equal to the second proportioning device
Times, to ensure to flow through the sample size of the sample size and the absorption of the second proportioning device 105 of flow chamber 108.
For example, it is assumed that the first proportioning device 103 flows through flow chamber 108 sample size from sample to flow chamber when pushing away is V, second is fixed
It is also V that amount device 105, which inhales sample and pushes away the sample size of sample, then the sample size that the first proportioning device 103 is drawn every time can be 2V
Integral multiple, you can be 2V, 4V or 6V etc., each sample amount that pushes away is 2V, the sample size that the first proportioning device 103 is drawn every time
Difference, the frequency for inhaling sample also can be different, and when the sample size that the first proportioning device 103 is drawn every time is 2V, action is to push away
Sample is primary, it is primary to inhale sample；When the sample size that the first proportioning device 103 is drawn every time be 4V when, action for push away sample twice, inhale sample
Once；When the sample size that the first proportioning device 103 is drawn every time be 6V when, action for push away sample three times, inhale sample it is primary, with such
It pushes away.
To become apparent from, explaining in detail the testing process of the streaming instrument to sample of the present embodiment, below with a kind of specific
Alternating push away sample flow and illustrate, include the following steps：
1）Inhale sample flow：When inhaling sample, first sample flow control valve 102 and the second sample flow control valve 104 are opened；Second is fixed
It measures device 105 and inhales sample, the sample size of absorption can make sample step the second sample flow control valve 104, and purpose makes subsequent quantitation first
When proportioning device 103 inhales sample, sample will not be diluted.Then the second sample flow control valve 104 is closed, the first proportioning device 103
Sample is inhaled, the sample size that the first proportioning device 103 is drawn is：3V, fast pushing volume and the second sample flow control valve 104 and flow chamber it
Between pipeline the sum of liquid storage amount, wherein V be the second proportioning device 105 it is each push away sample amount.In other specific embodiments, according to
The first suction amount of different control, the first proportioning device 103 can also be other values, such as：2V, fast pushing volume and the second sample
The sum of liquid storage amount of pipeline or 4V, fast pushing volume and the second sample flow control valve between this flow control valve 104 and flow chamber
The sum of the liquid storage amount of pipeline between 104 and flow chamber.While first 103 imbibition of proportioning device, the second proportioning device 105 is multiple
Position.
2）Fast plug-flow journey：After the completion of first proportioning device 103 inhales sample, first sample flow control valve 102 is closed, the second sample
Flow control valve 104 is opened；First proportioning device 103 pushes away sample, and sample is made to reach flow chamber rapidly, the first quantitative dress after the completion of pushing away soon
Set 103 sample sizes for also retaining 2V.While pushing away soon, the second proportioning device 105 inhales sample, suction amount V.Second proportioning device
105 suction samples must terminate before the first proportioning device 103 pushes away completion soon.
3）Measurement procedure：The completion of first proportioning device 103 postpones soon, and the second sample flow control valve 104 is closed, and second is quantitative
Device 105 pushes away sample by the flow of setting, and it is V to push away sample amount.After the completion of second proportioning device 105 pushes away sample, the second sample flow control
Valve 104 is opened, and the first proportioning device 103 pushes away sample with the speed of twice second proportioning device 105, and it is 2V to push away sample amount, meanwhile, the
Two proportioning devices 105 push away the speed of sample with it and inhale sample, suction amount V.When so that the first proportioning device 103 pushing away sample, into flowing
The sample flow of room, it is consistent with the second proportioning device 105.After the completion of first proportioning device 103 pushes away sample, the second sample flow control valve
104 close, and the second proportioning device 105 then pushes away sample with the speed of setting, and it is V to push away sample amount, while the first proportioning device 103 is inhaled
Sample, suction amount 2V, is recycled with this, is terminated until measuring.Sample flows out flow chamber after entering flow chamber under the package of sheath fluid
It finally collects in waste liquid pool 10.Optical system 11, which detects, flows through the sample stream information of flow chamber, obtain sample forward signal,
Lateral signal and fluorescence signal are handled by back end signal, calculate output final result.
Embodiment two：
Referring to FIG. 2, waste liquid absorption plant includes the waste liquid pool 210 and first for accommodating the waste liquid flowed out from flow chamber
Control valve 209, the first control valve 209 are arranged on the pipeline between waste liquid pool 210 to flow chamber 208 exports, and it is controllable
It opens or closes and switches between state；It includes being controlled for accommodating the liquid storage tank 206 and second of sheath fluid that sheath fluid, which provides device,
Valve 207, the second control valve 207 are arranged on liquid storage tank 206 to the pipeline between 208 entrance of flow chamber, and controllable are opening
Or it is switched between closed state.Proportioning device includes the first proportioning device 203 and the second proportioning device 205, and sample provides
Device is sampling needle 212, and sampling needle 212 can be inserted into tested sample pipe 201 and draw sample.First proportioning device 203 and second
Proportioning device 205 is arranged in parallel on the pipeline between flow chamber 208 and sampling needle 212, in the first proportioning device
Pipeline between 203 and sampling needle 212 is equipped with first sample flow control valve 2021, in the first proportioning device 203 and flow chamber
Pipeline between 208 is equipped with third sample flow control valve 2022, first sample flow control valve 2021, third sample flow control valve
2022 it is controllable switched between opening or closing state, the pipeline between the second proportioning device 205 and sampling needle 212
It is equipped with the second sample flow control valve 2041, the 4th sample is equipped on the pipeline between the second proportioning device 205 and flow chamber 208
This flow control valve 2042, the second sample flow control valve 2041, the 4th sample flow control valve 2042 it is controllable opening or closing shape
It is switched between state.Systems for optical inspection 211 is generally arranged at the opposite both sides of flow chamber 208, for emitting light beam and connecing
Receive the optical signal after sample acts on.
Since the first proportioning device 203 is connected in parallel with the second proportioning device 205 in flow chamber 208 and sampling
On pipeline between needle 212, therefore, the first proportioning device 203 and the second proportioning device 205 can respectively by sampling needle 212 from
Suction sample is carried out in tested sample pipe 201, is then alternately pushed away sample to flow chamber 208 again, is made to keep having sample flow always in flow chamber
It flows through.It can be that the first proportioning device 203 pushes away sample first, can also be that the second proportioning device 105 pushes away sample first.For example, two
Proportioning device alternately inhales sample and pushes away sample in a complementary fashion, i.e. when the first proportioning device 203 inhales sample, the second proportioning device
205 push away sample to flow chamber 208, and when the second proportioning device 205 inhales sample, the first proportioning device 203 pushes away sample to flow chamber 208.First
The each suction amount of proportioning device 203 and each suction amount of the second proportioning device 205 can be equal, can not also wait；First
Proportioning device 203 it is each push away sample amount and 205 each sample amount that pushes away of the second proportioning device can be equal, can not also wait.Below
By each suction amount of two proportioning devices and push away sample amount it is all equal for, illustrate using the streaming instrument in the present embodiment to sample
A kind of detailed process measured.
1）Inhale sample flow：When inhaling sample, first sample flow control valve 2021, the second sample flow control valve 2041 are opened；Third
Sample flow control valve 2022, the 4th sample flow control valve 2042 are closed, and the first proportioning device 203 and the second proportioning device 205 are inhaled
The sample size of sample, absorption can make sample enter proportioning device, it is therefore an objective to so that sample is respectively filled with sample tube 201 to the first quantitative
Pipeline between device 203 and the second proportioning device 205, the dilution in pipeline is drained, and when making subsequently to inhale sample, sample is not
It can be diluted.Then first sample flow control valve 2021 and the second sample flow control valve 2041 are closed, third sample flow control valve
2022, the 4th sample flow control valve 2042 and the first control valve 209 are opened, the first proportioning device 203 and the second proportioning device 205
It resets.Then third sample flow control valve 2022 and the 4th sample flow control valve 2042 are closed, first sample flow control valve 2021
It is opened with the second sample flow control valve 2041, the first proportioning device 203 and the second proportioning device 205 inhale sample, inhaled sample size energy
Support pushes away soon, and measures for the first time.
2）Fast plug-flow journey：After the completion of first proportioning device 203 and the second proportioning device 205 inhale sample, first sample flow control
Valve 2021 and the second sample flow control valve 2041 are closed, and third sample flow control valve 2022 and the 4th sample flow control valve 2042 are beaten
It opens；First proportioning device 203 and the second proportioning device 205 push away sample soon, make sample full of pipeline and reach flow chamber rapidly.Together
When, the second control valve 207 is opened, and the sheath fluid in liquid storage tank 206 is made to enter flow chamber.First proportioning device 203 and second is quantitative
Device 205 can be completed at the same time and push away soon, can also successively complete to push away soon.
3）Measurement procedure：First proportioning device 203 and the completion of the second proportioning device 205 postpone soon, one of those（Such as
First proportioning device 203）Stopping pushes away sample, another（Such as second proportioning device 205）Sample is pushed away by given flow velocity, second is quantitative
After the completion of device 205 pushes away sample, the first proportioning device 203 then pushes away sample at a same speed.Then the 4th sample flow control valve
2042 close, and the second sample flow control valve 2041 is opened, and the second proportioning device 205 inhales sample, and it is necessary that the second proportioning device 205 inhales sample
Terminate before the completion of the first proportioning device 203 pushes away sample.After the completion of first proportioning device 203 pushes away sample, the second proportioning device 205 connects
It and pushes away sample at a same speed.Then third sample flow control valve 2022 is closed, and first sample flow control valve 2021 is opened, and first
Proportioning device 203 inhales sample, and the first proportioning device 203, which inhales sample, to be terminated before the second proportioning device 205 is completed to push away sample.With this
Cycle terminates until measuring.Sample enters after flow chamber flows out flow chamber finally collection in waste liquid pool 210 under the package of sheath fluid
In.Systems for optical inspection 211, which detects, flows through the sample stream information of flow chamber, obtains the forward signal of sample, lateral signal and glimmering
Optical signal is handled by back end signal, calculates output final result.
In above-described embodiment, sample is inhaled by the joint of two or more proportioning devices and pushes away sample so that is quantitative every time
Device enters flow chamber sample flow when pushing away sample is consistent, and each propulsive liquid amount is also consistent, and can be constantly by sample
It is sent into flow chamber.Not only it can realize accurate metering, and can also realize and hold with the volume of accurate quantification tested sample in this way
It is continuous to measure.
According to present disclosure, it will be appreciated by those skilled in the art that waste liquid absorption plant and sheath fluid provide dress
It sets and other schemes realization that is existing or occurring in the future also can be used.Such as waste liquid absorption plant can also include waste liquid pool and can
The vacuum extractor for controlling switch opens vacuum plant when needing to receive waste liquid, so that waste liquid pool is formed subnormal ambient, makes to give up
Liquid stream enters waste liquid pool.Sheath fluid provide device can also be realized by the active conveying device of controllable switch, when need output sheath
It when liquid, turns on the switch, when sheath fluid need not be exported, turns off the switch.
The above content is combining, specific embodiment is made for the present invention to be further described, and it cannot be said that this hair
Bright specific implementation is confined to these explanations.For those of ordinary skill in the art to which the present invention belongs, it is not taking off
Under the premise of from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to the protection of the present invention
Range.
Claims (11)
1. a kind of liquid channel system of streaming instrument, it is characterised in that including：
Flow chamber sweeps along sample flow to pass through, and provides the place being irradiated by light for sample flow for accommodating sheath fluid；
Sample provides device, for drawing sample from tested sample pipe；
Sheath fluid provides device, pipeline connection to flow chamber, for sheath fluid to be transported to flow chamber；
Waste liquid absorption plant, pipeline connection to flow chamber, for receiving the waste liquid flowed out from flow chamber；
The pipeline of N number of proportioning device, each proportioning device is respectively communicated with flow chamber and sample provides device, and the N is more than
Or it is equal to 2, each proportioning device provides device from sample according to setting sequential and draws quantitative same type of sample liquid, and
Same type of sample is transported to flow chamber by least one proportioning device of each period according to the scheduled sample amount that pushes away, and makes to be tested
Sample flow does not flow through flow chamber interruptedly.
2. liquid channel system as described in claim 1, which is characterized in that the proportioning device is connected on flow chamber and sample provides
On pipeline between device.
3. liquid channel system as claimed in claim 2, which is characterized in that between two proportioning devices and proportioning device and
Sample is equipped with sample flow control valve between providing device, and sample flow control valve is controllable to be cut between opening or closing state
It changes.
4. liquid channel system as described in claim 1, which is characterized in that the proportioning device is connected in parallel on flow chamber and sample provides
On pipeline between device.
5. liquid channel system as claimed in claim 4, which is characterized in that fill between proportioning device and flow chamber and quantitatively
It sets and is equipped with sample flow control valve between sample offer device, sample flow control valve is controllable to be cut between opening or closing state
It changes.
6. the liquid channel system as described in any one of claim 1-5, which is characterized in that waste liquid absorption plant includes for accommodating
The waste liquid pool and the first control valve of the waste liquid flowed out from flow chamber, the first control valve setting are exported in waste liquid pool to flow chamber
Between pipeline on, and controllable switch between opening or closing state；It includes for accommodating sheath fluid that sheath fluid, which provides device,
Liquid storage tank and the second control valve, second control valve are arranged on liquid storage tank to the pipeline between flowing chamber inlet, and controllable
Switch between opening or closing state.
7. a kind of streaming instrument, including liquid channel system, Systems for optical inspection and controller, the liquid channel system is tested for providing
Sample flow waste liquid after sample flow and collection detection, the Systems for optical inspection is used to provide the light beam of irradiation sample flow, and obtains
Sampling originally flows through the information after light beam irradiation, which is characterized in that the liquid channel system includes：
Flow chamber sweeps along sample flow to pass through, and provides the place being irradiated by light for sample flow for accommodating sheath fluid；
Sample provides device, for drawing sample from tested sample pipe；
Sheath fluid provides device, pipeline connection to flow chamber, for sheath fluid to be transported to flow chamber；
Waste liquid absorption plant, pipeline connection to flow chamber, for receiving the waste liquid flowed out from flow chamber；
Two proportioning devices, two proportioning devices are connected on the pipeline between flow chamber and sample offer device, and first is quantitative
The entrance of device provides device with sample and is connected to, and the outlet of the second proportioning device is connected to flow chamber；
The controller is connect with two proportioning device signals respectively, and controlling two proportioning devices alternately continuously will be same type of
Sample is transported to flow chamber according to the scheduled sample amount that pushes away, and same type of tested sample stream is made to flow through flow chamber incessantly, and
When the controller, which controls the first proportioning device, pushes away sample, the second proportioning device of control is transported to from the first proportioning device in pipeline
Sample in carry out suction sample, and the suction sample of the second proportioning device is pushed away before sample terminates in the first proportioning device and is completed.
8. streaming instrument as claimed in claim 7, which is characterized in that between two proportioning devices and proportioning device and
Sample is equipped with sample flow control valve between providing device, and sample flow control valve is controllable to be cut between opening or closing state
It changes.
9. streaming instrument as claimed in claim 8, which is characterized in that the controller controls X proportioning device to flowing
It when room conveys sample, while controlling the X proportioning device and being opened to the sample flow control valve between flow chamber, control the X
A proportioning device provides first sample flow control valve on device path to sample and closes, and the X proportioning device is two
Any of a proportioning device.
10. streaming instrument as claimed in claim 7, which is characterized in that first proportioning device push away sample speed be more than or
Equal to 2 times that the second proportioning device pushes away sample speed, first proportioning device provides the sample size that device is drawn from sample and is equal to
The sample size that first proportioning device is conveyed to flow chamber is inhaled with the second proportioning device from the sample flow that the first proportioning device is released
The integral multiple of the sum of the sample size taken.
11. a kind of method carrying out particle analysis using streaming instrument, the streaming instrument includes liquid channel system, optical detection system
System and controller, the liquid channel system are used to provide tested sample stream and collect the sample flow waste liquid after detection, the optics inspection
Examining system is used to provide the light beam of irradiation sample flow, and obtains sample and flow through the information after light beam irradiation, the liquid channel system packet
Two proportioning devices are included, two proportioning devices are connected on the pipeline between flow chamber and sample offer device, the first quantitative dress
The entrance set provides device with sample and is connected to, and the outlet of the second proportioning device is connected to flow chamber, which is characterized in that the method
Including：Same type of sample is alternately continuously transported to flow chamber by two proportioning devices of control according to the scheduled sample amount that pushes away, and is made
Same type of tested sample stream flows through flow chamber incessantly, wherein at least includes：
It controls the first proportioning device and carries out suction sample according to scheduled first suction amount from sample offer device；
It controls the first proportioning device to push the first suction amount of absorption to flow chamber, when the first proportioning device pushes away sample, simultaneously
It controls the second proportioning device and carries out suction sample, and according to scheduled second suction amount from the sample flow that the first proportioning device is released
The suction sample of two proportioning devices, which is pushed away in the first proportioning device before sample terminates, to be completed；
It controls the second proportioning device to push the second suction amount of absorption to flow chamber, while controlling the first proportioning device from sample
Device is provided and carries out suction sample according to scheduled first suction amount, and the suction sample of the first proportioning device pushes away sample knot in the second proportioning device
It is completed before beam；
2 times for pushing away sample speed and pushing away sample speed more than or equal to the second proportioning device of first proportioning device, described first is fixed
The sample size that device is drawn from sample offer device is measured equal to the sample size that the first proportioning device is conveyed to flow chamber to determine with second
The integral multiple of the sum of the sample size that amount device is drawn from the sample flow that the first proportioning device is released.
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| CN103837462B (en) * | 2014-03-03 | 2016-10-05 | 中国科学院苏州生物医学工程技术研究所 | A kind of small-sized flow cytometer liquid-way system |
| CN109212246B (en) * | 2017-06-30 | 2022-06-24 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid supply device, sample analyzer, pressure building device and liquid supply method |
| WO2019127563A1 (en) * | 2017-12-30 | 2019-07-04 | 深圳迈瑞生物医疗电子股份有限公司 | Particle analyzer using sheath flow impedance and measurement method therefor |
| CN110749535B (en) * | 2019-10-15 | 2022-02-01 | 迈克医疗电子有限公司 | Flow cytometry detection system and method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093782C (en) * | 1996-07-26 | 2002-11-06 | 拜奥－多特公司 | Dispensing apparatus having improved dynamic range |
| CN101290313A (en) * | 2007-04-16 | 2008-10-22 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell device and method |
| CN102282453A (en) * | 2008-11-14 | 2011-12-14 | 贝克曼考尔特公司 | Monolithic optical flow cells and method of manufacture |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0531181A (en) * | 1991-08-02 | 1993-02-09 | Asahi Medical Co Ltd | Hemodialyzer |
| US6315952B1 (en) * | 1998-10-05 | 2001-11-13 | The University Of New Mexico | Plug flow cytometry for high throughput screening and drug discovery |
| DE102010040453A1 (en) * | 2010-09-09 | 2012-03-15 | Robert Bosch Gmbh | Exhaust gas aftertreatment system and method for metering a medium into the exhaust gas line of an internal combustion engine |
-
2012
- 2012-08-10 CN CN201210284882.XA patent/CN103575634B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093782C (en) * | 1996-07-26 | 2002-11-06 | 拜奥－多特公司 | Dispensing apparatus having improved dynamic range |
| CN101290313A (en) * | 2007-04-16 | 2008-10-22 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell device and method |
| CN102282453A (en) * | 2008-11-14 | 2011-12-14 | 贝克曼考尔特公司 | Monolithic optical flow cells and method of manufacture |
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