CN109959549A - Sample testing method and sample analyser - Google Patents
Sample testing method and sample analyser Download PDFInfo
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- CN109959549A CN109959549A CN201711429690.2A CN201711429690A CN109959549A CN 109959549 A CN109959549 A CN 109959549A CN 201711429690 A CN201711429690 A CN 201711429690A CN 109959549 A CN109959549 A CN 109959549A
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- 238000012360 testing method Methods 0.000 title claims abstract description 84
- 239000000523 sample Substances 0.000 claims abstract description 243
- 239000012470 diluted sample Substances 0.000 claims abstract description 126
- 238000006243 chemical reaction Methods 0.000 claims abstract description 103
- 239000012895 dilution Substances 0.000 claims abstract description 66
- 238000010790 dilution Methods 0.000 claims abstract description 66
- 238000001514 detection method Methods 0.000 claims abstract description 52
- 239000012472 biological sample Substances 0.000 claims abstract description 23
- 210000003743 erythrocyte Anatomy 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 20
- 238000005070 sampling Methods 0.000 claims description 9
- 238000004820 blood count Methods 0.000 claims description 8
- 238000002955 isolation Methods 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 9
- 239000012530 fluid Substances 0.000 description 9
- 238000006073 displacement reaction Methods 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 102000001554 Hemoglobins Human genes 0.000 description 3
- 108010054147 Hemoglobins Proteins 0.000 description 3
- 239000012898 sample dilution Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- INGWEZCOABYORO-UHFFFAOYSA-N 2-(furan-2-yl)-7-methyl-1h-1,8-naphthyridin-4-one Chemical compound N=1C2=NC(C)=CC=C2C(O)=CC=1C1=CC=CO1 INGWEZCOABYORO-UHFFFAOYSA-N 0.000 description 1
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 102000010445 Lactoferrin Human genes 0.000 description 1
- 108010063045 Lactoferrin Proteins 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
- 229940078795 lactoferrin Drugs 0.000 description 1
- 235000021242 lactoferrin Nutrition 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000000790 scattering method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses a kind of sample testing method, comprising: is once diluted in the first reaction tank to biological sample, to form a diluted sample；Sample lines are driven to extract a diluted sample by driving device；The diluted sample of part one time in sample lines is pushed into the second reaction tank by driving device；Secondary dilution is carried out to a diluted sample in the second reaction tank, to form secondary dilution sample；And detection secondary dilution sample.Above-mentioned sample testing method testing result accuracy is high.
Description
Technical field
The present invention relates to medical detection technology more particularly to a kind of sample testing methods and sample analyser.
Background technique
In existing blood analyser, the measurement side that red blood cell (Red Blood Cell, RBC) is counted is carried out to blood sample
Method is generally divided following several: optical method is measured by the sheath stream type of focusing and laser scattering method；Sheath-flow impedance method passes through
The sheath stream type of focusing and impedance method measure；And general impedance method, it is not necessarily to sheath stream, is directly surveyed using impedance method principle
Amount.
In above-mentioned measurement method, optical method and sheath-flow impedance method cost of implementation are higher, and cost is relatively low for general impedance method, but
It is required that Sample Dilution multiple is larger.Since Sample Dilution multiple is high, if there are flaws during Sample Dilution, it is easy to
Cause testing result inaccurate.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of sample testing method that testing result accuracy is high and
Sample analyser.
To achieve the goals above, embodiment of the present invention adopts the following technical scheme that
On the one hand, a kind of sample testing method is provided, comprising:
Biological sample is once diluted in the first reaction tank, to form a diluted sample；
Sample lines are driven to extract a diluted sample by driving device；
The part diluted sample in the sample lines is pushed into the second reaction tank by the driving device；
Secondary dilution is carried out to the diluted sample in second reaction tank, to form secondary dilution sample；
And
Detect the secondary dilution sample.
Wherein, the volume for the diluted sample for driving sample lines to extract by driving device is the first volume,
The volume that the driving device is pushed into the diluted sample in second reaction tank is the second volume, second body
The ratio of long-pending and described first volume is more than or equal to 0.5 and is less than or equal to 0.9.
Wherein, the ratio of second volume and first volume is more than or equal to 0.6 and is less than or equal to 0.7.
Wherein, the process for detecting the secondary dilution sample includes: to carry out red blood cell count(RBC) to the secondary dilution sample
Detection.
Wherein, the volume for the diluted sample for driving the sample lines to extract by the driving device is the
One volume, the volume that the driving device is pushed into the diluted sample in second reaction tank is the second volume；
After a diluted sample is pushed into second reaction tank, the sample testing method further include: pass through
The diluted sample of third volume in the sample lines is pushed into third reaction tank by the driving device, and described the
Three volumes and second volume and be less than first volume.
Wherein, the third volume and the sum of second volume and the ratio of first volume are more than or equal to 0.5 and small
In equal to 0.9.
Wherein, the sample testing method further include:
Secondary dilution is carried out to the diluted sample in the third reaction tank, to form the first diluted sample again
This；With
To described first again diluted sample carry out count detection.
Wherein, after a diluted sample is pushed into second reaction tank, the sample testing method further include:
A diluted sample remaining in the sample lines is all pushed into third reaction tank by the driving device.
Wherein, described to adopt before driving the sample lines to extract a diluted sample by the driving device
It include dilution in sample pipeline, a remaining diluted sample fully enters the third reaction in the sample lines
Chi Hou continues through the driving device for the part dilution in the sample lines and is pushed into the third reaction tank.
Wherein, the sample testing method further include:
Secondary dilution is carried out to the diluted sample in the third reaction tank, to form the second diluted sample again
This；With
To the described second diluted sample progress ratio detection again.
Wherein, described before driving the sample lines to extract a diluted sample by the driving device
Sample testing method further include:
By the driving device extraction section air, in end of the sample lines far from the driving device
Form isolation air column.
Wherein, the driving device includes syringe, and the syringe is extracted or released with pre-set velocity described primary dilute
Release sample.
On the other hand, a kind of sample analyser is also provided, including the first reaction tank, the second reaction tank, agent delivery component,
Driving device, sample lines, electrical impedance detector and controller, the controller are used for:
It controls the agent delivery component once to dilute the biological sample in first reaction tank, to form one
Secondary diluted sample；
It controls the driving device driving sample lines and extracts a diluted sample；
It controls the driving device and the part diluted sample in the sample lines is pushed into the second reaction tank；
It controls the agent delivery component and secondary dilution is carried out to the diluted sample in second reaction tank,
To form secondary dilution sample；And
It controls the electrical impedance detector and detects the secondary dilution sample.
Wherein, the volume for the diluted sample for driving sample lines to extract by driving device is the first volume,
The volume that the driving device is pushed into the diluted sample in second reaction tank is the second volume, second body
The ratio of long-pending and described first volume is more than or equal to 0.5 and is less than or equal to 0.9.
Wherein, the volume for the diluted sample for driving the sample lines to extract by the driving device is the
One volume, the volume that the driving device is pushed into the diluted sample in second reaction tank is the second volume, institute
Stating sample analyser further includes third reaction tank, and the controller is also used to:
After a diluted sample is pushed into second reaction tank, the driving device is controlled by the sampling pipe
The diluted sample of third volume in road is pushed into third reaction tank, the third volume and second volume and
Less than first volume.
Wherein, the sample analyser further includes third reaction tank, and the controller is also used to:
After a diluted sample is pushed into second reaction tank, the driving device is controlled by the sampling pipe
A remaining diluted sample is all pushed into third reaction tank in road.
Wherein, the controller is also used to: extracting the primary dilution controlling the driving device driving sample lines
Before sample, the driving device extraction section air is controlled, in end of the sample lines far from the driving device
Place forms isolation air column.
Wherein, the driving device includes syringe, so that the sample lines extract or release a diluted sample
This flow velocity and volume is controllable.
Wherein, the sample analyser further includes output unit, and the output unit is electrically connected the controller, described defeated
Unit is used to detect signal according to the electrical impedance of secondary dilution sample and obtains and export the red blood cell count(RBC) of the biological sample out
As a result.
Herein described sample testing method and the sample analyser, by only by the part institute in the sample lines
It states a diluted sample and is pushed into second reaction tank, to improve the secondary dilution sample formed in second reaction tank
Sample quality make the detection of the sample testing method and the sample analyser to guarantee the accuracy of testing result
Result precision is high.
Detailed description of the invention
In order to illustrate more clearly of technical solution of the present invention, attached drawing needed in embodiment will be made below
Simply introduce, it should be apparent that, the accompanying drawings in the following description is only some embodiments of the present invention, general for this field
For logical technical staff, without creative efforts, other attached drawings can also be obtained such as these attached drawings.
Fig. 1 is a kind of structural schematic diagram of sample analyser provided in an embodiment of the present invention；
Fig. 2 is a kind of correspondence schematic diagram of the part steps of sample testing method provided in an embodiment of the present invention；
Fig. 3 is a kind of flow chart of the step 01 to 05 of sample testing method provided in an embodiment of the present invention；
Fig. 4 is a kind of flow chart of the step 001 to 004 of sample testing method provided in an embodiment of the present invention；
Fig. 5 is a kind of flow chart of the step 06 to 08 of sample testing method provided in an embodiment of the present invention；
Fig. 6 is a kind of step 06 of sample testing method provided in an embodiment of the present invention ' to 08 ' flow chart.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention is described, and shows
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Referring to Fig. 1, the embodiment of the present invention provides a kind of sample testing method.The embodiment of the present invention also provides a kind of sample
Analyzer 100.The sample testing method detects biological sample using the sample analyser 100.The biology sample
It originally can be blood etc..The sample analyser 100 includes driving assembly, sampling component, reaction component, detection components, waste liquid
Processing component and controller 3.The driving component is used to drive various flow paths (including the gas in the sample analyser 100
Road and fluid path).The sampling component is for acquiring and distributing biological sample.The reaction component be used for the biological sample into
Row processing is to form prepare liquid.The detection components are for detecting the prepare liquid to form detection information.The liquid waste processing
Component is used to collecting and discharging the waste liquid in the sample analyser 100.The controller 3 is for controlling the sample analyser
100 workflow simultaneously handles the detection information to form analysis result.
Wherein, the sampling component includes sample lines 1.The driving component includes driving device 2, the driving device
2 for driving the sample lines 1 to extract or releasing fluid (including gas and liquid).The controller 3 is electrically connected the drive
Dynamic device 2, the controller 3 control the drive actions of the driving device 2.The reaction component includes the first reaction tank 4, the
Two reaction tanks 5 and third reaction tank 7.The reaction component further includes agent delivery component, and the controller 3 controls the reagent
Plurality of reagents of the provisioning component supply including dilution is into reaction tank, to be mixed with the sample in reaction tank, instead
It answers.The detection components 8 include electrical impedance detector 81, the electrical impedance detector 81 to by using impedance method principle into
Row measurement, cost is relatively low.The detection components 8 are electrically connected the controller 3, and the controller 3 controls the detection components 8
Detection operation.
Also referring to Fig. 1 to Fig. 3, the sample testing method includes:
Step 01: biological sample 10 once being diluted in the first reaction tank 4, to form a diluted sample 20.
Step 02: driving sample lines 1 to extract a diluted sample 20 by driving device 2.As D is signified in Fig. 2
To schematic diagram shown in.
Step 03: being pushed into the part diluted sample 20 in the sample lines 1 by the driving device 2
Second reaction tank 5.Shown in the schematic diagram as pointed by E and F in Fig. 2, schematic diagram pointed by E represents a diluted sample
20 are released into the process of second reaction tank 5, after the representative release of schematic diagram pointed by F in the sample lines 1
Also there are a part diluted samples 20.
Step 04: secondary dilution is carried out to the diluted sample 20 in second reaction tank 5, it is secondary to be formed
Diluted sample.
Step 05: detecting the secondary dilution sample.
In order to save sample, reduce testing cost, those skilled in the art are used whole one in the sample lines 1
All the second reaction tank 5 of push-in is diluted to obtain the scheme of secondary dilution sample secondary diluted sample.However, obtained by the program
To the testing result of secondary dilution sample be easy to appear higher phenomenon.To solve this problem, those skilled in the art usually adopt
With the accuracy for reinforcing cleaning way raising testing result.However, applicant is multiple it was found that, even if being added
Strong cleaning, for the result of detection there are still higher phenomenon, accuracy in detection is unsatisfactory, and the consumption of dilution increases too much.
Applicant repeatedly gropes in test to have been surprisingly found that subsequent, when a diluted sample in the sample lines 1 not exclusively pushes away
When entering second reaction tank 5, a diluted sample in second reaction tank 5 is diluted obtained secondary dilution sample
This testing result is accurate.
On this basis, applicant is had found by in-depth study analysis, the leading portion sample 20a in the sample lines 1
(diluted sample 20 for the sample lines 1 is divided into leading portion sample according to the sequence for entering the sample lines 1
20a and back segment sample 20b) it is easy a small amount of sample contamination for being detected by last time remaining in the sample lines 1, and back segment sample
20b is then hardly contaminated.And in herein described sample testing method, since the driving device 2 is only adopted described
A part diluted sample 20 in sample pipeline 1 is pushed into second reaction tank 5, therefore also protects in the sample lines 1
There are the leading portion sample 20a namely the sample testing method to be easy the contaminated leading portion sample 20a not by removal
Detection is participated in, and the back segment sample 20b is hardly contaminated by detection, the accuracy of testing result to guarantee, so
The testing result accuracy of the sample testing method is high.
Optionally, the driving device 2 includes syringe 21 and the motor 22 for driving syringe 2 mobile.The electricity
Machine 22 is electrically connected the controller 3.The controller 3 is by controlling the motor 22 to control 2 withdrawn fluid of syringe
Or release the volume and flow velocity of fluid.The sample lines 1 extract or release the flow velocity and body of a diluted sample at this time
Product is controllable, and the diluted sample 20 in the sample lines 1 is enabled quantitatively to be assigned to different reaction tanks
In, to realize repeated dispensing, improve detection speed.
The sample testing method further include: the syringe 21 is extracted or released a diluted sample with pre-set velocity
This.Since the controller 3 can control 21 withdrawn fluid of syringe by the motor 22 or release the flow velocity of fluid, because
This described syringe 21 can be extracted with pre-set velocity or release a diluted sample, to avoid the high-speed punching because of fluid
It brushes and is carried out the residual blood sample adsorbed in the sample lines 1, further improve the inspection of the sample testing method
Survey the accuracy of result.
Certainly, in other embodiments, other components can also be used in the driving device 2.For example, the driving device
Including multiple constant displacement pumps, such as two, volume be may be the same or different.It is successive that two constant displacement pumps are controlled by the controller 3
A dilution is sucked, the constant displacement pump drain sucked afterwards is realized so that the multiple constant displacement pump cooperates and extracts determined volume
Fluid or discharge determined volume fluid, to meet the driving demand of the sample testing method.Certainly, the multiple constant displacement pump
There can also be other fit systems, such as a successively dilution of sucking, the constant displacement pump drain first sucked.
Optionally, Fig. 1 to Fig. 4 is please referred to, before step 01, the sample testing method further include:
Step 001: driving the sample lines 1 to extract biological sample from sample container 6 by the driving device 2
10.The sample container 6 can be heparin tube.Shown in the schematic diagram as pointed by A in Fig. 2.Enter institute in the biological sample 10
It include dilution 30 in the sample lines 1 before stating sample lines 1.It is set between the biological sample 10 and dilution 30
There is air column, the biological sample 10 and dilution 30 is isolated, so that the testing result of the sample testing method is more accurate.
Step 002: being pushed into the quantitative biological sample 10 in the sample lines 1 by the driving device 2
First reaction tank 4.Shown in the schematic diagram as pointed by B in Fig. 2.
Step 003: cleaning the sample lines 1.Shown in the schematic diagram as pointed by C in Fig. 2.When cleaning sample lines 1,
The region that the inner wall and outer wall of the sample lines 1 contacted biological sample 10 is required to be cleaned.Dilution 30 can be passed through
It is cleaned.
Wherein, between step 002 and step 003, the sample testing method further include: pass through the driving device 2
The quantitative biological sample 10 in the sample lines 1 is pushed into other reaction tanks (such as the 4th reaction tank).At this point,
The biological sample 10 can carry out different processing (such as dilution and/or reaction) in different reaction tanks, to realize more
The different detection of kind.
It is understood that the sample analyser 100 further includes moving assembly, for driving the sample lines 1 to move
It is dynamic.For example, the moving assembly drives the sample lines 1 in the sample container 6, first reaction tank 4, described second
It is moved between reaction tank 5 and other reaction tanks.
Optionally, the volume for the diluted sample 20 for driving sample lines 1 to extract by driving device 2 is first
Volume.The volume that the driving device 2 is pushed into the diluted sample 20 in second reaction tank 5 is the second volume.
The ratio of second volume and first volume is more than or equal to 0.5 and is less than or equal to 0.9.At this point, the sample testing method
Both sample easy to pollute can have been removed not have to, while the use ratio of high quality samples can also be improved as far as possible, saved detection
Cost.Preferably, for further increase the sample testing method testing result accuracy, second volume with it is described
The ratio of first volume is more than or equal to 0.6 and is less than or equal to 0.7.
Optionally, the process for detecting the secondary dilution sample includes: to carry out red blood cell meter to the secondary dilution sample
Number detection.In other words, the detection in step 05 includes red blood cell (Red Blood Cell, RBC) count detection.Wherein, can lead to
It crosses the electrical impedance detector 81 and red blood cell count(RBC) detection is carried out to the secondary dilution sample, to reduce the pattern detection side
The testing cost of method.
In the present embodiment, since in step S03, the driving device 2 is only by the part institute in the sample lines 1
It states a diluted sample 20 and is pushed into second reaction tank 5, therefore can guarantee the sample quality of the secondary dilution sample,
The i.e. described biological sample 10 still has reliable sample quality in the case where dilution for many times, therefore can pass through the resistance of low cost
Anti- method detection mode is detected, to reduce the testing cost of the sample analyser and the sample testing method.It can be with
Understand, the process for detecting the secondary dilution sample may also comprise other kinds of count detection or ratio detection.
Count detection described herein is the quantity of a certain substance in the detection biological sample 10.For example, red thin
Born of the same parents (Red Blood Cell, RBC) count, blood platelet (blood platelet, PLT) detects.The ratio detection refers to inspection
Survey the ratio of a certain substance in the biological sample 10.For example, detection glycosylated hemoglobin accounts for the ratio of whole hemoglobins.
Requirement of requirement of the count detection to sample quality than ratio detection to sample quality is higher.
Optionally, the sample testing method further include: blood is carried out to a diluted sample in first reaction tank 4
Lactoferrin (Hemoglobin, HGB) Concentration Testing.
Optionally, the sample analyser 100 further includes output unit 9.The output unit 9 is electrically connected the controller
3, the output unit 9 is used to detect signal according to the electrical impedance of secondary dilution sample and obtains and export the red of the biological sample
Cell counts.The output unit 9 may include at least one of display screen and printer.The output unit 9 is also used for
Export other testing results, such as hemoglobin concentration testing result.
It is understood that the controller 3 is also used to form life according to the electrical impedance detection signal of secondary dilution sample
The red blood cell count(RBC) of object sample as a result, and the red blood cell count(RBC) result is sent to the output unit 9 so that the output
Unit 9 is able to obtain and export the red blood cell count(RBC) result.
In one embodiment, it also referring to Fig. 1 to Fig. 3 and Fig. 5, is driven by the driving device 2 described
The volume for the diluted sample 20 that sample lines 1 extract is the first volume.It is anti-that the driving device 2 is pushed into described second
The volume for answering the diluted sample 20 in pond 5 is the second volume.
After a diluted sample 20 is pushed into second reaction tank 5, the sample testing method further include:
Step 06: by the driving device 2 by a diluted sample for the third volume in the sample lines 1
20 push-in third reaction tanks 7, the third volume and second volume and be less than first volume.
In the present embodiment, due to the third volume and second volume and be less than first volume,
A part diluted sample 20 is also remained in the sample lines 1.By the driving device 2 by the sample lines
A diluted sample 20 in 1 is pushed into second reaction tank 5 and the third reaction tank 7, and the sampling respectively
Also remain with a part diluted sample 20 in pipeline 1, thus the secondary dilution sample of second reaction tank 5 and
In the third reaction tank 7 described first again diluted sample can not only be detected simultaneously to improve detection speed, also
Sample quality with higher can guarantee the accuracy of institute's testing result.
Wherein, the third volume and the sum of second volume and the ratio of first volume are more than or equal to 0.5 and small
In equal to 0.9.Do not have at this point, the sample testing method can both remove sample easy to pollute, while can also mention as far as possible
The use ratio of high high quality samples saves testing cost.Preferably, the detection to further increase the sample testing method
As a result accuracy, the third volume and the sum of second volume and the ratio of first volume are more than or equal to 0.6 and small
In equal to 0.7.
Wherein, the sample testing method further include:
Step 07: secondary dilution being carried out to the diluted sample 20 in the third reaction tank 7, to form first
Diluted sample again.
Step 08: to described first again diluted sample carry out count detection.
In the present embodiment, due in step S06, also remaining with the part primary dilution in the sample lines 1
Sample 20, therefore the sample quality of the described first diluted sample again is higher, so can to described first again diluted sample into
Row count detection, and testing result accuracy is high.Described first again diluted sample can be carried out by electrical impedance detector 81
Detection.Certainly, also total in other embodiments, it can also be to the described first diluted sample progress ratio detection again in step S08.
It is understood that step 07 is after step 06, step 08 after step 07, step 06, step 07 and
Step 08 then with, without specific sequencing, can both be carried out simultaneously between step 05, can also one in front and one in back carry out.
In another embodiment, it also referring to Fig. 1 to Fig. 3 and Fig. 6, is pushed away by a diluted sample 20
After entering second reaction tank 5, the sample testing method further include:
Step 06 ': it is by the driving device 2 that a diluted sample 20 remaining in the sample lines 1 is complete
Portion is pushed into third reaction tank 7.
In the present embodiment, be pushed into the third reaction tank 7 a diluted sample 20 can be pushed into institute
The diluted sample 20 for stating the first reaction tank 4, which synchronizes, to be diluted, detects, so as to improve the pattern detection
The detection speed of method.Certainly, detection of the application not to the diluted sample 20 for being pushed into the third reaction tank 7
Act and be pushed into a diluted sample 20 for first reaction tank 4 detection operation carry out the stringent time before and after
It limits.
Wherein, before driving the sample lines 1 to extract a diluted sample 20 by the driving device 2, institute
Stating includes dilution 30 in sample lines 1.Step 06 further include: a remaining diluted sample in the sample lines 1
After sheet 20 fully enters the third reaction tank 7, the driving device 2 is continued through by the part institute in the sample lines 1
It states dilution 30 and is pushed into the third reaction tank 7.
In the present embodiment, it is reacted by the way that the dilution 30 in the sample lines 1 is pushed further into the third
Pond 7 improves the sample testing method pair so that a diluted sample 20 be avoided to remain in the sample lines 1
The utilization rate of sample, reduces testing cost.Meanwhile also reducing the difficulty and cost for cleaning the sample lines 1.
Wherein, the sample testing method further include:
Step 07 ': secondary dilution is carried out to the diluted sample 20 in the third reaction tank 7, to form the
Two diluted samples again.
Step 08 ': to the described second diluted sample progress ratio detection again.
In the present embodiment, due to the remaining diluted sample in step S06 ', in the sample lines 1
20 enter the third reaction tank 7, therefore the sample quality of the described second diluted sample again may be by slight sample
This residual or lose influence, so described second again diluted sample in step S08 ' be detected as ratio detection, thus
Sample is neither wasted, can also guarantee the high accuracy of testing result as far as possible.
It is understood that step 07 is after step 06, step 08 after step 07, step 06, step 07 and
Step 08 then with, without specific sequencing, can both be carried out simultaneously between step 05, can also one in front and one in back carry out.
Optionally, also referring to Fig. 1 to Fig. 4, the sample lines 1 are being driven to extract institute by the driving device 2
Before stating a diluted sample 20, the sample testing method further include:
Step 004: by the 2 extraction section air of driving device, to be filled in the sample lines 1 far from the driving
The end for setting 2 forms isolation air column 40.Wherein, to drive the sample lines 1 to extract by the driving device 2 described primary
It include dilution 30 in the sample lines 1 before diluted sample 20.The isolation air column 40 is for being isolated the dilution 30
With a diluted sample 20.
In the present embodiment, since step 004 forms the isolation air column 40, institute can be isolated in the isolation air column 40
Dilution 30 and a diluted sample 20 are stated, therefore can be avoided the dilution of dilution 30 or pollute the primary dilution
Sample 20, to be further ensured that the accuracy of the testing result of the sample testing method.
Wherein, the sample lines 1 include sampler 11 and are connected between the sampler 11 and the driving device 2
Hose 12.The isolation air column 40 is formed in end of the sampler 11 far from the hose 12.
The embodiment of the present invention has been described in detail above, specific case used herein to the principle of the present invention and
Embodiment is expounded, and the above description of the embodiment is only used to help understand the method for the present invention and its core ideas；
At the same time, for those skilled in the art can in specific embodiments and applications according to the thought of the present invention
There is change place, in conclusion the contents of this specification are not to be construed as limiting the invention.
Claims (18)
1. a kind of sample testing method characterized by comprising
Biological sample is once diluted in the first reaction tank, to form a diluted sample；
Sample lines are driven to extract a diluted sample by driving device；
The part diluted sample in the sample lines is pushed into the second reaction tank by the driving device；
Secondary dilution is carried out to the diluted sample in second reaction tank, to form secondary dilution sample；And
Detect the secondary dilution sample.
2. sample testing method as described in claim 1, which is characterized in that drive sample lines to extract by driving device
The volume of diluted sample is the first volume, and the driving device is pushed into described primary dilute in second reaction tank
The volume of sample is released as the second volume, the ratio of second volume and first volume is more than or equal to 0.5 and is less than or equal to
0.9, it is preferred that the ratio of second volume and first volume is more than or equal to 0.6 and is less than or equal to 0.7.
3. sample testing method as described in claim 1, which is characterized in that detect the process packet of the secondary dilution sample
It includes: red blood cell count(RBC) detection is carried out to the secondary dilution sample.
4. sample testing method as described in claim 1, which is characterized in that drive the sampling pipe by the driving device
The volume for the diluted sample that road is extracted is the first volume, and the driving device is pushed into the institute in second reaction tank
The volume for stating a diluted sample is the second volume；
After a diluted sample is pushed into second reaction tank, the sample testing method further include: by described
A diluted sample for third volume in the sample lines is pushed into third reaction tank, the third body by driving device
It is long-pending with second volume and be less than first volume.
5. sample testing method as claimed in claim 4, which is characterized in that the third volume and second volume and
And the ratio of first volume is more than or equal to 0.5 and is less than or equal to 0.9.
6. sample testing method as claimed in claim 4, which is characterized in that the sample testing method further include:
Secondary dilution is carried out to the diluted sample in the third reaction tank, to form the first diluted sample again；
With
To described first again diluted sample carry out count detection.
7. sample testing method as described in claim 1, which is characterized in that by diluted sample push-in described the
After two reaction tanks, the sample testing method further include: will be remaining described in the sample lines by the driving device
One time diluted sample is all pushed into third reaction tank.
8. sample testing method as claimed in claim 7, which is characterized in that drive the sampling pipe by the driving device
It include dilution, the remaining institute in the sample lines in the sample lines before a diluted sample is extracted on road
It states after a diluted sample fully enters the third reaction tank, continuing through the driving device will be in the sample lines
The part dilution is pushed into the third reaction tank.
9. sample testing method as claimed in claim 7, which is characterized in that the sample testing method further include:
Secondary dilution is carried out to the diluted sample in the third reaction tank, to form the second diluted sample again；
With
To the described second diluted sample progress ratio detection again.
10. such as sample testing method according to any one of claims 1 to 9, which is characterized in that passing through the driving device
Before driving the sample lines to extract a diluted sample, the sample testing method further include:
By the driving device extraction section air, to be formed in the sample lines far from the end of the driving device
Air column is isolated.
11. such as sample testing method according to any one of claims 1 to 9, which is characterized in that the driving device includes note
Emitter, the syringe are extracted or are released a diluted sample with pre-set velocity.
12. a kind of sample analyser, which is characterized in that including the first reaction tank, the second reaction tank, agent delivery component, driving
Device, sample lines, electrical impedance detector and controller, the controller are used for:
The agent delivery component is controlled once to dilute the biological sample in first reaction tank, it is primary dilute to be formed
Release sample；
It controls the driving device driving sample lines and extracts a diluted sample；
It controls the driving device and the part diluted sample in the sample lines is pushed into the second reaction tank；
It controls the agent delivery component and secondary dilution is carried out to the diluted sample in second reaction tank, with shape
At secondary dilution sample；And
It controls the electrical impedance detector and detects the secondary dilution sample.
13. sample analyser as claimed in claim 12, which is characterized in that drive sample lines to extract by driving device
The volume of diluted sample is the first volume, and the driving device is pushed into described primary dilute in second reaction tank
The volume of sample is released as the second volume, the ratio of second volume and first volume is more than or equal to 0.5 and is less than or equal to
0.9。
14. sample analyser as claimed in claim 12, which is characterized in that drive the sampling pipe by the driving device
The volume for the diluted sample that road is extracted is the first volume, and the driving device is pushed into the institute in second reaction tank
The volume for stating a diluted sample is the second volume, and the sample analyser further includes third reaction tank, and the controller is also used
In:
After a diluted sample is pushed into second reaction tank, controlling the driving device will be in the sample lines
The diluted sample of third volume be pushed into third reaction tank, the sum of the third volume and second volume is less than
First volume.
15. sample analyser as claimed in claim 12, which is characterized in that the sample analyser further includes third reaction
Pond, the controller are also used to:
After a diluted sample is pushed into second reaction tank, controlling the driving device will be in the sample lines
A remaining diluted sample is all pushed into third reaction tank.
16. the sample analyser as described in any one of claim 12~15, which is characterized in that the controller is also used to:
Before controlling the driving device driving sample lines and extracting a diluted sample, the driving device extracting part is controlled
Divide air, to form isolation air column far from the end of the driving device in the sample lines.
17. the sample analyser as described in any one of claim 12~15, which is characterized in that the driving device includes note
Emitter so that the sample lines extract or release a diluted sample flow velocity and volume it is controllable.
18. the sample analyser as described in any one of claim 12~15, which is characterized in that the sample analyser also wraps
Output unit is included, the output unit is electrically connected the controller, and the output unit is used for the electricity according to secondary dilution sample
Impedance detection signal obtains and exports the red blood cell count(RBC) result of the biological sample.
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| CN201711429690.2A CN109959549A (en) | 2017-12-25 | 2017-12-25 | Sample testing method and sample analyser |
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| CN201711429690.2A CN109959549A (en) | 2017-12-25 | 2017-12-25 | Sample testing method and sample analyser |
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| WO2021147179A1 (en) * | 2020-01-22 | 2021-07-29 | 深圳市锦瑞生物科技有限公司 | Blood particle detection method and blood analyzer thereof |
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| WO2022134654A1 (en) * | 2020-12-21 | 2022-06-30 | 深圳市帝迈生物技术有限公司 | Sample dilution method and device, detection method and device, and storage medium |
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