WO2022134654A1

WO2022134654A1 - Sample dilution method and device, detection method and device, and storage medium

Info

Publication number: WO2022134654A1
Application number: PCT/CN2021/116542
Authority: WO
Inventors: 方建伟
Original assignee: 深圳市帝迈生物技术有限公司
Priority date: 2020-12-21
Filing date: 2021-09-03
Publication date: 2022-06-30
Also published as: CN114646525A

Abstract

A sample dilution method, a detection device, a storage medium and a dilution device. The sample dilution method comprises: estimating at least one dilution ratio (S11); determining another dilution ratio according to a target dilution ratio of a sample and the at least one dilution ratio (S12); adjusting the at least one dilution ratio (S14) until the other dilution ratio meets preset requirements (S13); and determining that the current at least one dilution ratio and the other dilution ratio are dilution ratios used during one dilution process among multiple dilution processes of the sample, respectively (S15). By means of the foregoing manner, the dilution ratio accuracy of a target solution may be increased when multiple dilutions are carried out.

Description

Sample dilution method and device, detection method and device, storage medium

This application claims the priority of the Chinese patent application with the application number of 202011520180.8 and the patent title of "A sample dilution method, detection method, detection device and storage medium", the contents of which are incorporated in this application by reference.

【Technical field】

The present application relates to the field of medical technology, and in particular, to a sample dilution method and device, a detection method and device, and a storage medium.

【Background technique】

At present, the medical testing technology can automatically analyze various cells or biochemical components in the blood, and obtain the indicators of each component in the blood. When performing blood cell analysis, such as the analysis of red blood cells or platelets, the blood is usually diluted to a certain rate, and then the blood cells are counted.

To achieve accurate blood analysis, it is usually necessary to dilute the sample to a lower concentration, which may cause the sample and diluent to be taken beyond the range and accuracy of the sampling needle, resulting in an error between the obtained sample dilution ratio and the target dilution ratio. larger.

[Content of the invention]

The present application mainly provides a sample dilution method and device, a detection method and device, and a storage medium, which can solve the problem that it is difficult to balance the sample suction amount and the dilution liquid suction amount in multiple dilutions in the prior art.

In order to solve the above technical problems, a first aspect of the present application provides a sample dilution method. Wherein, the method includes the following steps: estimating at least one dilution ratio; determining another dilution ratio according to the target dilution ratio of the sample and the at least one dilution ratio; adjusting the at least one dilution ratio until the other dilution ratio A dilution ratio satisfies a preset requirement, and it is determined that the current at least one dilution ratio and the other dilution ratio are respectively the dilution ratios used in one dilution process in the multiple dilution processes of the sample.

In order to solve the above technical problems, a second aspect of the present application provides a sample detection device, the sample detection device includes a processor and a memory coupled to each other, and a computer program is stored in the memory, and the processor is used to execute the The computer program is used to implement the sample dilution method provided in the first aspect.

In order to solve the above technical problems, a third aspect of the present application provides a computer-readable storage medium, where the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, realizes the sample dilution provided in the first aspect above. method.

In order to solve the above technical problems, a fourth aspect of the present application provides a sample dilution device, including: a liquid addition level for loading a first dilution cup; a liquid addition mechanism, located above the liquid addition level, for sucking and diluting liquid and sample liquid, and added to the first dilution cup located at the liquid addition level to form a primary dilution sample liquid; the intermediate transfer position, located below the liquid addition mechanism, is used to receive the first dilution cup containing the first dilution the first dilution cup of the sample liquid; the liquid adding mechanism is also used for sucking the diluent and the first dilution sample liquid and adding them into the second dilution cup to form the second dilution sample liquid.

In order to solve the above technical problem, the fifth aspect of the present application provides a method for detecting a sample, the method comprising: determining a first dilution ratio and a second dilution ratio according to the method provided in the first aspect; The dilution ratio sucks the diluent and the sample liquid, and spit the sucked liquid into the first dilution cup to obtain the first solution; according to the first dilution ratio, suck the diluent and the first solution, and spit the sucked liquid into the first dilution cup. Two dilution cups are obtained to obtain a second solution; the second solution is detected.

The beneficial effects of the present application are: different from the situation in the prior art, the present application first estimates at least one of the dilution ratios, and then uses the target dilution ratio and the estimated at least one dilution ratio data to determine another dilution ratio, and according to a preset It is required to judge whether the obtained dilution ratio meets the requirements. If it does not meet the requirements, then at least one estimated dilution ratio is adjusted again, and another dilution ratio is re-determined until the preset requirements are met. By continuously adjusting the estimated dilution ratio, the value of the dilution ratio used for each dilution is more in line with the preset requirements, so that the optimal dilution ratio of each dilution step can be found, and the loss of samples and diluents caused by the dilution ratio data can be reduced. The error caused by the misalignment of the added sample volume makes the final dilution ratio of the sample solution more in line with the target dilution ratio.

【Description of drawings】

In order to illustrate the technical solutions in the embodiments of the present application more clearly, the following briefly introduces the drawings that are used in the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without creative effort. in:

FIG. 1 is a schematic block diagram of a flow chart of an embodiment of the sample dilution method of the present application;

2 is a schematic flow chart of another embodiment of the sample dilution method of the present application;

3 is a schematic block diagram of the flow of another embodiment of the sample dilution method of the present application;

FIG. 4 is a schematic block diagram of the flow of an embodiment of the present application for determining a preset requirement;

5 is a schematic structural diagram of an embodiment of the sample dilution device of the present application;

6 is a schematic diagram of an embodiment of the dilution process of the present application;

7 is a schematic structural diagram of an embodiment of a transfer assembly of the present application;

8 is a schematic diagram of an embodiment of the transport path of each component of the dilution process of the present application;

9 is an exploded schematic view of an embodiment of the transfer assembly of the present application;

10 is a schematic structural diagram of an embodiment of the rotating shaft in FIG. 9 of the present application;

11 is a schematic structural diagram of another embodiment of the rotating mechanism of the present application;

12 is a schematic block diagram of the circuit structure of an embodiment of the sample analyzer of the present application;

13 is a schematic block diagram of a flow chart of an embodiment of a method for detecting samples of the present application;

14 is a schematic block diagram of a flow chart of another embodiment of the method for detecting samples of the present application;

FIG. 15 is a schematic block diagram of the flow of another embodiment of the detection method of the sample of the present application;

16 is a schematic block diagram of the circuit structure of an embodiment of the sample detection device of the present application;

FIG. 17 is a schematic block diagram of a circuit structure of an embodiment of a computer-readable storage medium of the present application.

【Detailed ways】

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

The terms "first" and "second" in this application are only used for descriptive purposes, and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features shown. Thus, a feature delimited with "first", "second" may expressly or implicitly include at least one of that feature. Furthermore, the terms "comprising" and "having", and any conjugations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device comprising a series of steps or units is not limited to the listed steps or units, but optionally also includes unlisted steps or units, or optionally also includes For other steps or units inherent to these processes, methods, products or devices.

Reference herein to an "embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor a separate or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

In the test items that need to be tested after dilution of blood and other samples, when a sample dilution with a lower concentration is to be obtained, the ratio of the required dilution to the required sample is extremely large. If the dilution is completed at one time, it may lead to dilution. The amount of liquid taken exceeds the range of the sampling needle, or the amount of sample taken cannot reach the accuracy of the sampling needle, which eventually leads to problems such as inaccurate dilution ratio of the obtained target solution. Therefore, the accuracy of the dilution ratio of the target solution can be improved by multiple dilutions. Wherein, in the multiple dilution process, each dilution step uses the solution obtained in the previous dilution step as a sample for dilution.

The present application proposes a method for diluting a sample, which is used to determine the dilution ratios used for multiple dilutions. Specifically, another dilution ratio is calculated by estimating one dilution ratio. Each dilution process is diluted with one dilution ratio or another dilution ratio, and a target solution that meets the target dilution ratio is finally obtained after multiple dilutions.

Please refer to FIG. 1. FIG. 1 is a schematic block diagram of a flow chart of an embodiment of a method for diluting a sample of the present application. The sample dilution method of this embodiment includes the following steps:

S11: Estimate at least one dilution ratio.

The dilution ratio, that is, the ratio between the amount of sample and the amount of solution obtained by dilution, can be expressed as:

The amount of the sample and the amount of the diluent are the volume of the sample taken and the volume of the diluent, respectively.

The dilution ratio data for each dilution step of the dilution process has the following relationship:

γ=γ ₁ *...*γ _n ………………(1)

Wherein, γ represents the target dilution ratio, γ ₁ , . . . , γ _n are the dilution ratio data used in multiple dilution steps, respectively, and n is an integer greater than or equal to 2.

Wherein, in this step, at least one dilution ratio can be estimated according to the number of dilutions and the target dilution ratio, for example, the dilution ratios of multiple dilution steps can be determined as a close numerical value or an equivalent numerical value, thereby determining the estimated value of at least one dilution ratio, It is also possible to determine an estimated value of a dilution ratio by taking the minimum dilution error of the current dilution step as a target, and those skilled in the art can also estimate at least one dilution ratio in other ways. For example, when the sample needs to be diluted twice, one of the dilutions can be estimated to determine another dilution ratio. When the sample needs to be diluted three times or more, a certain dilution ratio can be determined by estimating multiple dilutions. For the dilution ratio of one dilution process, for example, in three dilution processes, by estimating the dilution ratio of the second and third dilution processes, the appropriate dilution ratio of the first dilution process is determined step by step.

S12: Determine another dilution ratio according to the target dilution ratio of the sample and at least one dilution ratio.

The target dilution ratio is the rate at which the sample in the target solution is diluted. Another way of determining the dilution ratio can be determined by formula (1).

S13: Determine whether another dilution ratio meets the preset requirement.

The preset requirements are set, and after another dilution ratio is calculated in step S12, it is determined whether it meets the conditions through this step.

If another dilution ratio meets the preset requirement, step S15 is performed, otherwise, step S14 is performed.

S14: Adjust at least one dilution ratio.

Adjust each dilution ratio data in at least one dilution ratio with a preset step size, and return to step S12 to recalculate another dilution ratio. The adjustment direction may be increasing or decreasing, and the adjustment step size of each dilution ratio data may be the same or different.

S15: Determine that the current at least one dilution ratio and another dilution ratio are respectively the dilution ratios used in one dilution process in the multiple dilution processes of the sample.

The above method is used to determine at least one dilution ratio and another dilution ratio, wherein at least one dilution ratio is the dilution ratio of one or several dilutions in multiple dilution processes, and the other dilution ratio is the dilution ratio of the remaining dilution processes.

After at least one dilution ratio and another dilution ratio are obtained, the sample dosage and the amount of the diluent under the corresponding dilution ratio are calculated respectively, so as to facilitate the dilution operation of the corresponding step according to the amount of the sample and the amount of the diluent.

Wherein, when the at least one dilution ratio includes multiple dilution ratios, after another dilution ratio is determined, the dilution ratio data in the at least one dilution ratio can be further optimized. Specifically, the partial dilution in the at least one dilution ratio can be estimated. The ratio data is used to determine one of the dilution ratio data, and stepwise optimization is performed according to steps S11 to S15 to obtain each dilution ratio data.

In this way, when the target dilution is relatively small, the dilution process can be divided into multiple dilution steps to improve the sampling accuracy of the sampling needle, thereby reducing the dilution ratio error of the target solution. Moreover, the multiple dilution process of the present application only calculates two dilution ratio data, and the processing amount of the data is small.

Optionally, before step S11, it is judged whether the target dilution ratio is smaller than the preset dilution ratio, and if it is smaller than the preset dilution ratio, steps S11 to S15 are executed to calculate the dilution ratio multiple times; Calculation of multiple dilution ratios. Specifically, the smaller the value of the target dilution ratio, the greater the difference between the amount of the sample to be taken and the diluent, and the accuracy can be improved by performing multiple dilutions.

Wherein, the other dilution ratio includes a first dilution ratio, the at least one dilution ratio includes a second dilution ratio, and the first dilution ratio and the second dilution ratio are respectively the dilutions used in one dilution process among the two dilution processes of the sample. Compare.

That is, the first dilution ratio may be estimated in step S11, and the second dilution ratio may be determined according to the first dilution ratio and the target dilution ratio in step S12; the second dilution ratio may also be estimated in step S11, and step S12 is The first dilution ratio is determined according to the second dilution ratio and the target dilution ratio.

In one embodiment, the dilution of the sample is divided into two dilution steps, namely the first dilution and the second dilution. The first dilution process uses the first dilution ratio, and the second dilution process uses the is the second dilution ratio. The sampling objects of the first dilution are the first sample and the diluent, the sampling objects of the second dilution are the solution and the diluent obtained by the first dilution, and the target solution is obtained by the second dilution.

Please refer to FIG. 2 and FIG. 3 together. FIG. 2 is a schematic flowchart of another embodiment of the sample dilution method of the present application, and FIG. 3 is a schematic flowchart of the embodiment.

The dilution step in this embodiment includes the first dilution and the second dilution. For the first dilution, ρa ₁ μL of the first sample and ρb ₁ μL of the dilution solution are taken, and the first dilution is used to obtain a diluted solution; To a ₂ μL of the second sample and b ₂ μL of the diluent, the second dilution obtains the target solution, wherein the second sample is obtained from the solution after the first dilution.

The volume of the solution obtained from the first dilution can be expressed as:

V ₁ =ρa ₁ +ρb ₁ ……………………(2)

where ρ is the scaling factor.

The volume of the target solution obtained from the second dilution can be expressed as:

V ₂ =a ₂ +b ₂ ……………………(3)

The first dilution ratio can be expressed as:

The second dilution ratio can be expressed as:

The sample dilution method of this embodiment includes the following steps:

S21: Estimate the second dilution ratio.

In this step, the dilution ratio can be preset as the estimated value of the second dilution ratio.

For example, in one embodiment, 0.5 is used as the estimated value of the second dilution ratio. Specifically, when the second dilution ratio is 0.5, the volume of the second sample taken during dilution is equal to the volume of the diluent taken. For the second dilution step, there will be no measurement accuracy and range beyond the sampling needle. risk with high accuracy. Those skilled in the art can completely preset another value as the estimated value of the second dilution ratio.

S22: Determine the first dilution ratio according to the target dilution ratio and the second dilution ratio of the sample.

The first dilution ratio is determined by the following relationship:

γ=γ ₁ ·γ ₂ ……………………(6)

Among them, γ is the target dilution ratio, γ ₁ is the first dilution ratio, γ ₂ is the second dilution ratio, and the target dilution ratio γ and the second dilution ratio γ ₂ are both known quantities, which can be calculated by formula (6). The first dilution ratio γ ₁ .

S23: Determine whether the first dilution ratio meets the preset requirement.

If the _first dilution ratio γ1 meets the preset requirement, step S25 is performed; otherwise, step S24 is performed to adjust the second dilution ratio.

The preset requirements may be determined through the following steps S31-S32.

S31: Obtain: the volume of the target solution, the volume range of the solution obtained in the first dilution process, and the proportional relationship between the volume of the first sample used in the first dilution process and the volume of the second sample used in the second dilution process.

This step determines the volume of the target solution as V, where:

V=V ₂ =a ₂ +b ₂ ……………………(7)

The volume range of the solution obtained during the first dilution process is:

V _min ≤V ₁ ≤V _max ……………………(8)

The proportional relationship between the volume of the first sample used in the first dilution process and the volume of the second sample used in the second dilution process is:

ρa ₁ =La ₂ …………………………(9)

S32: Determine the preset requirement by using the volume of the target solution, the volume range of the intermediate sample, the first dilution ratio, the target dilution ratio, and the proportional relationship.

The preset requirements are determined using the following formula:

Among them, V _min is the minimum value of the solution volume range obtained by the first dilution, V _max is the maximum value of the solution volume range obtained by the first dilution, V is the volume of the target solution, γ ₁ is the first dilution ratio, γ ₂ is the second dilution ratio, and L is the ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process.

If the _first dilution ratio γ1 calculated in step S22 satisfies the condition of the formula (10), it is considered that the _first dilution ratio γ1 meets the preset requirement.

Wherein, V _min , V _max and V can be determined by those skilled in the art according to the volume of the used dilution container or the range of the sampling needle.

Specifically, step S32 utilizes the volume V of the target solution, the intermediate sample volume range V _min to V _max , the first dilution ratio γ ₁ , the target dilution ratio γ, and the proportional relationship L to determine the preset requirements as follows:

Combining formulas (2) and (4), we can get:

ρa ₁ =γ ₁ ·V ₁ ……………………(11)

Combining equations (5) and (7), we can get:

a ₂ =γ ₂ ·V……………………(12)

Combining formulas (9), (11) and (12), we can get:

Combined with equations (8) and (13), the preset condition of equation (10) can be obtained.

Optionally, the ratio between the volume of the first sample used in the first dilution process and the volume of the second sample used in the second dilution process is 1:1, that is, L=1. At this time, the preset conditions are:

The advantage of setting the ratio of the volume of the original sample used in the first dilution process to the volume of the intermediate sample used in the second dilution process to 1:1 in this embodiment is that the volume of the sample used in the two dilution processes is equal, it can minimize the sample addition error and improve the dilution accuracy.

S24: Adjust the second dilution ratio.

When the first dilution ratio does not meet the preset requirement, the second dilution ratio is adjusted with a preset adjustment step. Optionally, the second dilution ratio is decreased with a preset adjustment step size of 0.1 until the first dilution ratio meets the preset requirement.

After adjusting the dilution ratio in this step, return to step S22 to re-determine the first dilution ratio until the first dilution ratio meets the preset requirement.

In another embodiment, step S24 may also be to adjust the volume of the intermediate sample to be used for the second dilution. Specifically, the volume of the intermediate sample to be used for the second dilution may be adjusted according to an integer multiple of the sampling needle graduation value. volume a ₂ . Among them, the division value is the value that can be measured between two adjacent scale values on the sampling needle or sampling syringe.

S25: Determine the current first dilution ratio as the dilution ratio used in the first dilution process, and determine the current second dilution ratio as the dilution ratio used in the second dilution process.

After the first dilution ratio and the second dilution ratio are determined, the first sample dosage ρa ₁ and the diluent dosage ρb ₁ for the first dilution can be determined according to formulas (9) and (12), and the formulas (5) and ( 12) Determine the second sample amount a ₂ and the diluent amount b ₂ for the second dilution.

Through the above method, the present embodiment can calculate the appropriate first dilution ratio and second dilution ratio. For the first and second dilutions, respectively, to improve the accuracy of each dilution step.

In another embodiment, the above method can also be used to determine: the dilution ratio data of each dilution step in the dilution process with more than two dilution steps. Among them, the dilution ratio data relationship of multiple dilution processes is: γ=(α)·(β), where α and β are both integers greater than or equal to 1, and α+β is the number of times to be diluted, and γ represents the target dilution Ratio, γ′ and γ″ are the dilution ratios of each dilution process, wherein the same dilution ratio data is used for some dilution processes, and when one of α and β is greater than 1 or both are greater than 1, it can be determined according to the above Steps S21～S25 determine the dilution ratio data of each step. Specifically, under the dilution step with the dilution ratio of γ′, it is determined that the volume of the diluted solution to be obtained in each dilution step is equal, and all satisfy the formula (8) Under the dilution step with the dilution ratio of γ″, it is determined that the volume of the diluted solution to be obtained in each dilution step is equal to the preset value V, and γ′ and γ″ are respectively used as steps S21 to S25. The first dilution ratio and the second dilution ratio, in this way, γ′ and γ″ can be determined using steps S21 to S25 . For example, in the dilution process for 3 dilutions, the dilution ratios of the first dilution and the second dilution can be made equal, and the dilution ratio is γ′, and the dilution ratio of the third dilution is γ″, then it can be determined by determining The volumes of the solutions obtained from the first dilution and the second dilution are equal and satisfy the volume range of formula (8). The volume of the diluted solution to be obtained from the third dilution is the preset value V. As the first dilution ratio and the second dilution ratio in steps S21-S25, the method of steps S21-S25 is used to determine the dilution ratio γ' of the first dilution and the second dilution, and the dilution ratio γ" of the third dilution .During the dilution process of 3 dilutions, the dilution ratio of the first dilution can also be made γ′, the dilution ratio of the second dilution and the third dilution can be γ″, and it is determined that the volume of the solution obtained by the first dilution satisfies In the volume range of formula (8), the volume of the solution to be obtained by the third dilution is the preset value V, and similarly, γ′ and γ″ can be respectively used as the first dilution ratio and the second dilution ratio in steps S21 to S25 , using the methods of steps S21 to S25 to determine the dilution ratio γ′ of the first dilution, and the dilution ratios γ″ of the second dilution and the third dilution.

Or, when more than three dilutions are required, for example, during n dilutions, the dilution ratio data of each dilution step are γ ₁ , γ ₂ , . . . , γ _n respectively, where γ=γ ₁ *γ ₂ *.. .*γ _n , γ is the target dilution ratio, and one of the dilution ratio values can be determined by partial dilution ratio values. For example, when n=3, γ ₂ and γ ₃ can be determined first, and γ ₁ can be obtained. According to γ ₁ If the preset requirements are met, adjust γ ₂ and γ ₃ until γ ₁ meets the preset requirements. γ ₂ and γ ₃ can also be optimized after γ ₁ is determined. In this way, multiple dilution ratio data can be obtained step by step. Meet the requirements of various dilution times.

Please refer to FIG. 5 , which is a schematic structural diagram of an embodiment of a sample dilution device of the present application. The sample diluting device 10 is provided with a liquid filling level 100, an intermediate transfer position 200 and a liquid addition mechanism 30, wherein the liquid addition level 100 is used to load the first dilution cup, and the liquid addition mechanism 30 is arranged above the liquid addition level 100 for sucking and diluting liquid and sample liquid, and add the sucked diluent and sample liquid into the first dilution cup located at the liquid addition level to form a primary dilution sample liquid. The intermediate transfer position 200 is arranged below the liquid addition mechanism 30 for receiving The first dilution cup for the primary dilution of the sample solution, the liquid adding mechanism 30 is also used for sucking the diluent and the primary dilution sample liquid and adding them to the second dilution cup to form the secondary dilution sample liquid.

Wherein, the primary dilution sample solution is the diluted sample solution obtained by the first dilution of the sample, and the second dilution sample solution is the diluted sample solution obtained by the second dilution of the sample (the sample solution obtained after the previous dilution). The sample dilution device 10 can also perform more than two dilution operations, for example, three dilutions, four dilutions, or even five dilutions and more dilution operations.

The liquid adding mechanism 30 may be a structure that can quantitatively transfer liquid, such as a sample adding needle, and the amount of sample liquid and diluent that the liquid adding mechanism draws each time is based on the amount of sample liquid and diluent required for each dilution step. implement.

Specifically, the liquid addition level 100 is a set position, which is a fixed position in space. At the set position, the liquid addition mechanism 30 can be set as: above the set position, The liquid adding mechanism 30 discharges the sucked sample liquid and the diluting liquid, so that the sucked sample liquid and the diluting liquid are added to the dilution cup located at the liquid adding level 100 .

The intermediate position 200 is another set position. In the secondary dilution process, it is used to load the dilution cup containing the first dilution sample solution, and in the three or more dilution processes, it can be used to load the intermediate The dilution cup for diluting the sample liquid, so that in the next dilution step, the liquid adding mechanism 30 can suck the intermediate diluted sample liquid at the intermediate position 200 to perform the next dilution. The intermediate diluted sample solution is the diluted sample solution obtained in the dilution step before the last dilution. For example, when three dilutions are to be performed, in the operation sequence, the intermediate position 200 is used to place the dilution cup containing the first dilution sample liquid and the dilution cup containing the second dilution sample liquid in turn. The diluted sample solution is to be used for detection, and the transfer position 200 does not transfer it.

The intermediate transfer position 200 can be set at a position adjacent to the liquid addition level 100, so that the liquid addition mechanism 30 can move to the liquid addition position 100 for the next dilution after sucking the intermediate diluted sample liquid in the intermediate transfer position.

In one embodiment, the sample diluting device 10 further includes a rotating mechanism 20, the rotating mechanism 20 includes a fixed base 21 and a rotating base 22, the rotating base 22 is arranged in the fixed base 21 and can rotate relative to the fixed base 21, the rotating base 22 There are a plurality of dilution cup holes 220 on the outer edge of the swirl cup, and the dilution cup holes 220 will pass through the filling level 100 when rotating with the rotating seat 22. In this way, the dilution cup loaded in each dilution cup hole 220 can follow the rotating seat 22. The rotation of 22 is sequentially driven to the liquid addition level 100, and the liquid sucked by the liquid addition mechanism 30 can be received at the liquid addition level 100, and each dilution step is performed in sequence.

Wherein, when a dilution cup is located at the liquid addition level 100, the cup mouth end of the dilution cup is arranged in a protruding shape, so as to facilitate the picking and placing, and the operation of transferring or mixing. In a feasible embodiment, a cushion block is provided at the bottom of the fixed seat 21 corresponding to the position of the filling level 100, and the cushion block has two inclined surfaces along the circumferential direction of the rotation of the rotary seat 22, regardless of whether the rotary seat 22 rotates in a counterclockwise or clockwise direction. , When the dilution cup is driven to rotate to the filling level 100, the dilution cup can be gradually raised, so that the mouth end of the cup protrudes from the hole of the dilution cup, which is convenient for taking and placing. The dilution cups that appear in this document can be the first dilution cup, the second dilution cup, or any dilution cup of any dilution process, if not specified.

For example, in the second dilution process, the rotating seat 22 drives the first dilution cup located in one of the dilution cup holes 220 to the liquid addition level 100 by rotating, and the liquid addition mechanism 30 adds the sucked sample liquid and diluent to the first dilution cup. The dilution cup is obtained once the dilution liquid is obtained, the first dilution cup is transferred to the intermediate position 200, the rotating seat 22 is rotated, and the second dilution cup located in one of the dilution cup holes 220 is driven to the liquid addition level 100, and the liquid addition mechanism 30 is located in the intermediate position. The first dilution cup at position 200 sucks the first dilution sample liquid, and the diluent is sucked in the container containing the diluent, and the sucked first dilution sample liquid and the dilution liquid are added to the second dilution cup at the liquid addition level 100, and two dilutions are obtained. Secondary dilution. If more dilutions are to be performed, after the liquid adding mechanism 30 has finished sucking the diluted sample solution, the first dilution cup is discarded, the intermediate position 200 is vacated, and the second dilution cup is transferred after obtaining the second dilution. 200, the rotating seat 22 rotates to drive the third dilution cup located in one of the dilution cup holes 220 to the liquid addition level 100, and the liquid addition mechanism 30 sucks the diluted sample liquid once in the second dilution cup located at the intermediate position 200, and sucks the dilution liquid , add the one-time dilution sample solution and the diluted solution to the third dilution cup located at the liquid level 100 to obtain the third dilution solution. If more dilutions are required, the coordination of each component can be controlled according to the above operation sequence. Let's go into details.

In a feasible embodiment, the rotating mechanism 20 includes a motor 23, the motor 23 is relatively fixedly arranged on the fixed base 21, and the rotating base 22 is connected to the output end of the motor 23, so as to drive the rotating base 22 clockwise or clockwise through the rotation of the motor 23. By rotating counterclockwise, the dilution cup hole 220 can be rotated to the liquid addition level 100 in sequence, and the dilution cup can be automatically loaded to the liquid addition level 100 . The dilution cup holes 220 are evenly distributed on the edge of the rotating seat 22, and the dilution cup holes 220 can be placed at the liquid filling level 100 in sequence by controlling the motor 23 to rotate by a preset angle each time. For example, the rotating seat 22 shown in FIG. The four dilution cup holes 220 are evenly distributed on the edge of the rotating seat 22, and each time the rotating seat 22 is rotated by 90 degrees, each dilution cup hole 220 can be located at the filling level 100 in turn, so that the loading can be realized. The dilution cups in the dilution cup hole 220 are transported to the liquid addition level 100 in turn. Of course, the number of holes in the dilution cup can be set according to actual needs, so there is no specific limitation.

Wherein, the rotating mechanism 20 also includes a dilution cup flow channel 24, and the dilution cup flow channel 24 is provided with a accommodating groove 240, and the accommodating groove 240 is used for accommodating at least one dilution cup, and one end of the dilution cup flow channel 24 is connected to the cup adding mechanism ( Not shown), used to add a dilution cup to the accommodating tank 240, one end of the diluting cup flow channel 24 is connected to the rotating seat, when the accommodating tank 240 is communicated with at least one dilution cup hole 220, the dilution cup 240 is close to the dilution cup The dilution cup in the cup hole 220 is transferred to the dilution cup hole 220 to perform automatic filling of the dilution cup. The first detection photocoupler 25 is provided inside the accommodating groove 240 for detecting the dilution cups in the accommodating groove 240 , so as to control the quantity of the dilution cups in the accommodating groove 240 .

In a feasible embodiment, a second detection photocoupler 26 is provided on the side of the fixed seat 21 at a position corresponding to the liquid filling level 100, and the second detection photocoupler 26 is used to detect the position of the dilution cup at the liquid filling level 100, so as to control the filling level 100. operations such as liquid or dilution cup transfer.

In one embodiment, the dilution cup hole 220 will pass through the liquid filling level 100 and the intermediate transfer level 200 in sequence when the rotating seat 22 rotates. That is to say, the intermediate position 200 is set as a fixed position in space. When the rotating seat 22 rotates, the dilution cup holes 220 can be located in the intermediate position 200 in sequence, and the dilution cup holes 220 are first located at the liquid filling level 100 in time sequence. , and then at this intermediate position 200.

The dilution cup hole 220 in this embodiment can be located in the intermediate position 200 in turn with the rotation of the rotary base 22 , that is, the dilution cup hole 220 is used to carry the dilution cup containing the intermediate diluted sample solution, and there is no need to set up another station for the intermediate station. , which can make the internal structure of the dilution device more compact and improve the space utilization rate.

In a specific dilution process, please refer to FIG. 10 . FIG. 10 is a schematic diagram of an embodiment of the rotating seat of the present application. The first dilution cup 51 and the second dilution cup 52 are respectively located in one of the dilution cup holes 220. With the rotation of the rotary seat 22, the first dilution cup 51 can be driven to the liquid filling level 100, and the first dilution operation is performed, and the rotation is performed. The seat 22 rotates once to transfer the second dilution cup 52 to the liquid filling level 100 . At this time, the first dilution cup 51 is located at the intermediate transfer position 200 , and the second dilution operation is performed in the second dilution cup 52 . Wherein, the first dilution cup 51 may be driven to the intermediate position 200 along with the rotation of the rotating base 22 , or may be transferred to the intermediate position 200 by being clamped.

In a feasible embodiment, a spacer block is provided at the bottom of the fixed seat 21 corresponding to the position of the middle index 200, and the spacer block has two inclined surfaces along the circumferential direction of the rotation of the rotary seat 22, no matter whether the rotary seat 22 rotates counterclockwise or clockwise, When the dilution cup is driven to rotate to the intermediate position 200, the dilution cup can be raised gradually, so that the mouth end of the cup protrudes from the hole of the dilution cup, which is easy to be clamped; or when the dilution cup is placed in the intermediate position 200, The mouth end of the dilution cup protrudes from the hole of the dilution cup, which is convenient for taking and placing the dilution cup.

Among them, please continue to refer to FIG. 1, a third detection photocoupler 27 can be set at the position of the side of the fixing base 21 corresponding to the intermediate index 200, and the third detection optical coupler 27 is used to detect the presence or absence of the dilution cup of the intermediate index 200, so as to control the adding The liquid mechanism 30 performs liquid extraction operations and the like from the dilution cup at the intermediate index 200 .

Wherein, the sample dilution device 10 may further include a transfer assembly 40, and the transfer assembly 40 is disposed above the rotating mechanism 20 and can transfer the dilution cup. Please refer to FIG. 7 , which is a schematic structural diagram of an embodiment of a transfer assembly of the present application.

The transfer assembly 40 includes a motion mechanism 60 and a mixing mechanism 70. The motion mechanism 60 is used to enable the mixing mechanism 70 to grip and transfer the first dilution cup at the liquid filling level 100, and the mixing mechanism 70 is used to grip and transfer the first dilution cup at the liquid filling level 100. Mix the first dilution cup during the process. In three or more dilution processes, the transfer assembly 40 may also operate other dilution cups.

In another feasible embodiment, the intermediate position is not set at the position corresponding to the hole 220 of the dilution cup, and the mixing mechanism 70 may be the intermediate position. Specifically, in the secondary dilution process, the first dilution cup is placed in the mixing mechanism 70 , and during the secondary dilution operation, the liquid adding mechanism 30 directly sucks the primary dilution sample liquid on the mixing mechanism 70 . In this way, when the mixing mechanism 70 performs the mixing operation on the first dilution cup, the rotating mechanism 20 can be controlled to drive the second dilution cup to the liquid addition level 100, and after the first dilution cup is mixed, the liquid addition mechanism 30 can be controlled. Directly sucking the diluted sample liquid once on the mixing mechanism 70 does not need to set up another station as the intermediate transfer position, which can effectively save space, and there is no need to transfer the first dilution cup to another station for the liquid extraction operation, which can save operation time. Improve dilution efficiency.

Please refer to FIG. 8 , the dotted line in the figure represents the movement path of the transfer assembly 40 , and the intermediate transfer position 200 can be set at a certain position of the movement path of the transfer assembly 40 , so that when the dilution cup is transferred, the liquid adding mechanism 30 can be located in the transfer position. The intermediate dilution sample is aspirated from the dilution cup of assembly 40.

Wherein, the transfer assembly 40 can be a three-axis motion mechanism, which can move in the X direction and/or the Y direction and/or the Z direction relative to the liquid addition level 100. The intermediate transfer position 200 and the liquid addition level 100 are in the same line in the X direction or the Y direction. set up. The X-direction and the Y-direction in FIG. 8 are only for schematic illustration, and do not mean that the X-direction and the Y-direction are on the same plane. The Z-direction movement enables the vertical movement of the transfer assembly 40 to perform the pick-and-place operation of the dilution cup.

In a specific dilution process, please continue to refer to FIG. 8 , the solid line represents the motion path of the liquid adding mechanism 30, and the dotted line represents the motion path of the transfer assembly 40. Here, in order to clearly show the motion path of the liquid adding mechanism 30 and the transfer assembly 40, In the figure, the dotted line and the implementation are staggered. In actual operation, the path indicated by the solid line and the path indicated by the dotted line can be set to overlap. In the dilution process, the liquid adding level 100 loads the first dilution cup, the liquid adding mechanism 30 moves along the AB path, and passes through the diluent container 11 and the sample liquid container 12 in turn, respectively according to the required amount of diluent and sample. The liquid volume sucks the diluent and the sample liquid, and then moves along the BC path. When it reaches the liquid addition level 100, the suctioned liquid is added to the first dilution cup; the transfer assembly 40 moves the first dilution cup out of the liquid addition level 100, and mixes it. Evenly, move along the path CE to the middle index position 200, control the rotation of the rotary base 22, and transfer the second dilution cup to the liquid addition level 100; after adding the liquid into the first dilution cup, the liquid addition mechanism 30 moves along the CA path and here Clean up during the process, then move along the AB path, through the container 11 containing the diluent, absorb the diluent quantitatively in the container 11, then move along the BC path, and quantitatively absorb a dilution from the first dilution cup after passing through the intermediate position 200 For the sample liquid, add the sucked liquid into the second dilution cup when the liquid addition level 100 is passed to obtain the second dilution sample liquid; after the liquid addition mechanism 30 draws the first dilution sample liquid from the first dilution cup located at the intermediate transfer position 200, the transfer assembly 40 can be moved along the EDC, discarding the first dilution cup in the process. If the second dilution is carried out, mix the second dilution cup and transfer it to the detection area for detection. If there is a dilution step, control each component to continue to operate according to the coordination of the above components until the target is obtained. Diluent. In this way, the intermediate dilution sample liquid can be sucked in the paths of mixing, transporting and discarding of the dilution cup, without having to transport the dilution cup to a specific intermediate position, and the various components of the sample dilution device 10 can be efficiently coordinated , to improve the dilution efficiency, on the other hand, there is no need to set up another intermediate transfer position, and the space utilization rate is improved.

Wherein, the path setting in this embodiment is only a schematic illustration, and the moving direction and transfer path of each component may not be as described in FIG. 8 . Besides, there may be other ways. The movement direction of the 100 is not limited to the X direction and the Y direction, and there may be various ways, which will not be listed here.

7 and 9, FIG. 9 is an exploded schematic diagram of an embodiment of the transfer assembly of the present application.

The transfer assembly 40 includes a motion mechanism 60 and a mixing mechanism 70 , wherein the mixing mechanism 70 includes a limit block 71 , a gripper slider 72 , a gripper assembly 73 and a rotating shaft 74 , wherein the limit block 71 is provided with a limit slot 710 , one end of the gripper slider 72 is in clearance fit with the limit groove 710, and the other end is fixedly connected with the gripper assembly 73. The gripper assembly 73 is used to grab the dilution cup, and the rotating shaft 74 is sleeved with the gripper slider 72 in a gap so as to The gripper slider 72 and the gripper assembly 73 are driven to shake by the rotation of the rotating shaft 74 , so as to realize the operation of mixing the samples in the dilution cup.

The gripper slider 72 is provided with a first limit hole 721 and a second limit hole 722. The first limit hole 721 is used to fit the aforementioned rotating shaft 74 to drive the gripper to slide when the rotating shaft 74 rotates. The block 72 shakes in the limiting groove 710 . The motion mechanism 60 is arranged on one side of the limit block 71 , and the motion mechanism 60 can drive the limit block 71 to drive the gripper slider 72 to move axially relative to the rotating shaft 74 , and then drive the gripper assembly 73 relative to the rotating shaft through the gripper slider 72 . 74 performs axial movement.

The mixing mechanism 70 further includes a limit pin 711 disposed in the limit block 71 and an eccentric driven wheel 712 sleeved on the limit pin 711 , and the eccentric driven wheel 712 is sleeved with the second limit hole 722 . Wherein, there is a first eccentricity between the secondary axis of the rotating shaft 74 and the main axis, the axis of the limit pin 711 and the axis of the eccentric driven wheel 712 have a second eccentricity, and the first eccentricity is less than or equal to the second Eccentricity. In this embodiment, an eccentric driven wheel 712 is sleeved on the gripper slider 72 to cooperate with the rotation when the rotating shaft 74 rotates, so as to improve the response sensitivity of the gripper slider 72 .

In a feasible embodiment, the mixing mechanism 70 further includes a bottom frame 75, a top frame 76, and a guide rod 77 connected between the bottom frame 75 and the top frame 76. The limit block 71 and the gripper slider 72 are based on the guide rod 77. The rod 77 and the rotating shaft 74 are movably arranged between the bottom frame 75 and the top frame 76 . Specifically, through holes 1100 are formed on both sides of the limit block 71 respectively, and the guide rod 77 is penetrated through the through holes 1100. When the limit block 71 and the gripper slider 72 are driven by external force, the bottom frame 75 and the top frame can be driven Axial movement in the area between 76. The bottom frame 75 is also fixedly provided with a bearing 82 that transitionally fits with the rotating shaft 74 , and one end of the rotating shaft 74 close to the bottom frame 75 is matched with the bearing 82 to rotate.

Please refer to FIG. 10 . FIG. 10 is a schematic structural diagram of an embodiment of the rotating shaft shown in FIG. 9 of the present application. The rotating shaft 74 includes a main shaft section 741, a transition section 742 and a secondary shaft section 743 connected in sequence, wherein the main shaft section 741 and the secondary shaft section 743 have the same outer diameter, and the outer diameter of the transition section 742 is smaller than the main shaft section 741 and the secondary shaft section 743. Outer diameter, and when the rotating shaft 74 and the gripper slider 72 are assembled, there is a certain gap at the position where the rotation shaft 74 is sleeved with the gripper slider 72, and the gripper slider 72 moves axially relative to the rotation shaft 74 from the transition section 742. The auxiliary shaft section 743 can realize the mixing operation of the dilution cup, so as to prevent the mixing device from being stuck due to the influence of the outer diameter of the transition section 742 in the process of switching the operation state. The transition section 742 is truncated, and the cross-sectional area of the side of the transition section 742 connected to the main shaft section 741 and the auxiliary shaft section 743 is the same as the cross-sectional area of the main shaft section 741 and the auxiliary shaft section 743 .

Further, the axis line of the secondary shaft segment 743 is not on the same straight line as that of the primary shaft segment 741 , that is, the secondary axis of the secondary shaft segment 743 is eccentrically arranged relative to the primary axis of the primary shaft segment 741 . Since the rotating shaft 74 and the gripper sliding block 72 are gapped and sleeved, when the rotating shaft 74 is driven by an external force, the rotating shaft 74 rotates based on the main axis. The gripper slider 72 shakes in the limiting groove 710 and then shakes in conjunction with the gripper assembly 73, so as to perform a mixing action on the dilution cup. When the gripper slider 72 is located on the main shaft section 741, the dilution cup can be gripped, or the captured dilution cup can be released and put down or discarded.

In a feasible embodiment, please continue to refer to FIG. 9 , the limiting block 71 includes a bottom block 714 and a surface block 715 , and the limiting groove 710 is located between the bottom block 714 and the surface block 715 .

In a feasible embodiment, the mixing mechanism 70 further includes an optocoupler 78, and a blocking piece 713 corresponding to the optocoupler 78 is provided on the limiting block 71. The optocoupler 78 is used to obtain the in-position state of the blocking piece 713 to correspond to Determine the in-position state of the gripper slider 72 relative to the secondary shaft segment 743 . Specifically, when the optocoupler 78 detects the blocking plate 713 , the gripper slider 72 is in the secondary shaft section 743 , otherwise, the gripper slider 72 is not in the secondary shaft section 743 .

In a feasible embodiment, the mixing mechanism 70 further includes a rotary motor 79, and the output end of the rotary motor 79 is connected to the secondary shaft segment 743, please refer to FIG. The docking hole 744 is used for docking with the output end of the rotating electrical machine 79, so that the output end of the rotating electrical machine 79 is set corresponding to the docking hole 744 on the main shaft section 741 and the auxiliary shaft section 743. When the rotary motor 79 is working, the rotating shaft 74 rotates with the main shaft as the rotation center in response to the rotation of the rotary motor 79, and then drives the gripper slider 72 and the gripper assembly 73 to shake through the secondary shaft section 743, so as to dilute the clamping cup to mix. Wherein, the rotating motor 79 may be directly fixed and installed, or may be connected to the rotating shaft 74 through a motor fixing base 83 disposed between the rotating motor 79 and the limiting block 71 .

In a feasible embodiment, the gripper assembly 73 includes a gripper claw that can be elastically opened and closed, and the gripper jaw includes a first gripper 731 and a second gripper 732 that are oppositely arranged, and the first gripper 731 and the second gripper 732 Working together, it is used to hold the dilution cup. The gripper assembly 73 further includes: a limiting member 733 , a guide member 734 and an elastic member 735 , wherein the first gripper 731 and the second gripper 732 pass through the guide member 734 to slide along the guide member 734 to make the first gripper 734 The gripper 731 and the second gripper 732 are close to or away from each other to grab or release the dilution cup, and the elastic member 735 is arranged between the first gripper 731 and the second gripper 732 for connecting the first gripper 731 and the The second gripper 732 and the elastic member 735 can be stretched or contracted by external force, so that the first gripper 731 and the second gripper 732 are in a clamping state to clamp the dilution cup. The limiting member 733 is arranged on the outside of the first gripper 731 and the second gripper 732 to limit the sliding space of the first gripper 731 and the second gripper 732 on the guide member 734 and avoid the first gripper 731 and the second gripper 732 . The gripper 732 comes off. In a feasible embodiment, the elastic member 735 is a spring member.

The motion mechanism 60 includes a lift motor 61, a driving wheel 62 connected to the output end of the lift motor 61, a driven wheel 63 spaced from the driving wheel, a synchronous belt 64 sleeved on the driving wheel 62 and the driven wheel 63, The link 65 connecting the belt 64 to the limit block 71 is used to drive the synchronous belt 64 to rotate forward and reverse, and then the limit block 71 is linked by the link 65 to move axially relative to the guide rod 77 and the shaft 74 . Wherein, the linkage 65 presses and connects the synchronous belt 64 on the limit block 71, so that the limit block 71 moves synchronously with the synchronous belt 64, and drives the gripper slider 72 to move along the axial direction of the rotating shaft 74, so that the The gripper slide 72 can be in the main shaft section 741 or the secondary shaft section 743 .

In an embodiment, the linkage member 65 includes a first pressing and fixing member 651 , and the first pressing and fixing member 651 is directly used to press-fit and connect the synchronous belt 64 to the limiting block 71 .

In another embodiment, the linkage member 65 includes a first pressing and fixing member 651 and a second pressing and fixing member 652 , wherein a groove is defined on the side of the first pressing and fixing member 651 close to the second pressing and fixing member 652 . , the part of the synchronous belt 64 used to connect the limit block 71 is accommodated in the groove, and the synchronous belt 64 is partially fixed in the groove by the first pressing fixing piece 651 and the second pressing fixing piece 652, and then is connected to the limiter on bit block 71. The linkage 65 can be fixedly connected to the timing belt 64 and the limit block 71 by means of bolts or screws, or can be fixedly connected by snap fit, as long as the linkage 65 can drive the limit block 71 to move.

Further, the mixing mechanism 70 may include a protection member 80 disposed on the top frame 76 and corresponding to the exposed part of the rotating motor 79 for protecting the rotating motor 79 and other components of the mixing mechanism 70 .

Further, the mixing mechanism 70 may include a dilution cup detection photocoupler 81, and the dilution cup detection photocoupler 81 is arranged on the base frame 75 at a position opposite to the direction of the clamping jaws to detect the clamping jaws or the dilution cup clamped by the clamping jaws, To determine whether the gripper slider 72 is located in the main shaft section 741 .

The transfer assembly 40 of this embodiment has the functions of transfer and mixing at the same time, which can significantly improve the efficiency of transfer and mixing during the dilution process, especially the operation process of multiple dilutions.

In other embodiments, the intermediate position may not be set at the position corresponding to the dilution cup hole 220 , nor at the mixing mechanism 70 . For example, the rotating mechanism 20 can be embedded in the cup-carrying table (not shown), and the cup-carrying table can be provided with other working areas or stations where the dilution cups are placed, so that the dilution device can be operated in coordination with other working areas. For example, a middle indexing position (not shown in the figure) can be set on the cup carrier table. When the dilution cup needs to be transferred, the dilution cup is transferred to the middle indexing position on the cup carrier table. The liquid adding mechanism 30 only needs to take the liquid at the intermediate position on the cup-carrying table.

Among them, the intermediate position is a counterbore recessed on the surface of the cup-carrying table. The counterbore can be a round hole or a square hole. The round hole can be used to carry the optical cup, and the square hole can be used to carry the magnetic bead cup. The depth of the counterbore is smaller than the height of the first dilution cup or the second dilution cup, so that the cup mouth end of the dilution cup located in the counterbore can protrude from the surface of the cup carrier table, which is convenient for taking and placing the dilution cup.

Please refer to FIG. 11 , which is a schematic structural diagram of another embodiment of the rotating mechanism of the present application. The rotating mechanism 20 of this embodiment includes a fixed base 21 and a rotating base 22. The rotating base 22 is provided with a plurality of dilution cup holes 220. The liquid filling level 100 is set corresponding to one of the dilution cup holes 220, and the dilution cup hole 220 is used for loading the dilution cup. , the rotating seat 22 can be rotated clockwise or counterclockwise to drive the dilution cup located in the dilution cup hole 220 to the liquid filling level 100 in sequence. In this embodiment, the intermediate position 300 is set in the fixing base 21, and the intermediate position 300 is a recessed counterbore set on the surface of the fixing base 21. Similar to the previous embodiment, the intermediate position 300 is used for loading the dilution cup containing the intermediate diluted sample solution. , so that in the next dilution step, the liquid adding mechanism 30 can suck the intermediate dilution sample liquid at the intermediate position 300 to perform the next dilution.

Wherein, the counterbore of the intermediate index 300 can be a square hole or a round hole, the round hole can be used to carry an optical cup, and the square hole can be used to carry a magnetic bead cup.

Alternatively, the fixing base 21 is provided with two intermediate positions at the same time, wherein, the two intermediate positions are both set as recessed counterbores, and the counterbore of one of the intermediate positions is a square hole, which is used for the transfer of the magnetic bead cup, and the other The counterbore of the intermediate position is a round hole, which is used to transfer the optical cup. The structure of this embodiment can select the intermediate position according to actual needs. For example, please continue to refer to Fig. 11, the two intermediate positions are the intermediate position 300 and the intermediate position 400, the counterbore of the intermediate position 300 is a round hole, and the counterbore of the intermediate position 400 is a square hole. The intermediate transfer position 300 is used for the transfer of the dilution cup. When the magnetic bead cup needs to be used for the dilution operation, the intermediate transfer position 400 is selected for the transfer of the dilution cup, which is suitable for the dilution operation of various detection items, and can improve the utilization rate of the sample dilution device 10.

The depth of the counterbore in the two intermediate positions is smaller than the height of the first dilution cup or the second dilution cup. In short, the cup mouth end of the dilution cup located at the

intermediate position

300 or 400 can protrude from the surface of the fixing seat 21, which is convenient for picking and placing. That's it.

In the above-mentioned various embodiments of the present application, the transfer assembly 40 can be used to perform mixing and transfer operations on the dilution cup. For example, in one embodiment, the operation flow of the dilution process for a sample is as follows: when the first dilution cup is driven to the liquid addition level 100 by the rotating mechanism 20 , the liquid addition mechanism 30 moves to the first dilution cup at the liquid addition level 100 Above, the sucked diluent and sample solution are added to the first dilution cup, and a dilution sample liquid is obtained in the first dilution cup, and then the transfer assembly 40 moves to the top of the first dilution cup at the liquid addition level 100, and passes through the first dilution cup. The mixing mechanism 70 of the transfer assembly 40 clamps the first dilution cup, and drives the motion mechanism 60 to move the gripper slider 72 to the secondary shaft section 743 of the rotating shaft 74. After the movement, the bottom of the first dilution cup can completely avoid the position of the first dilution cup. The height of the dilution cup at the liquid level 100 controls the movement of the rotating mechanism 20 to drive the second dilution cup to the liquid addition level 100, and the rotating shaft 74 is driven to rotate by the rotary motor 79, thereby mixing the primary dilution sample liquid in the first dilution cup. , to complete a dilution, move the first dilution cup to the position for subsequent detection for detection. If there is a need for secondary dilution, transfer the first dilution cup to the intermediate transfer position, which can be the intermediate transfer position in any of the above-mentioned embodiments. At this time, the second dilution cup is located at the liquid addition level 100, which drives the liquid addition mechanism. 30 moves to the intermediate position, sucks the diluted sample liquid once in the first dilution cup, the liquid addition mechanism 30 also sucks the diluent in the diluent holding container, and moves the liquid addition mechanism 30 to the liquid addition level 100, and the liquid addition mechanism 30 The sucked liquid is added to the second dilution cup located at the liquid addition level 100, and the second sample dilution liquid is obtained in the second dilution cup. After the liquid addition mechanism 30 sucks the first dilution sample liquid in the first dilution cup, it drives the transfer assembly 40. Transfer the first dilution cup to the cup throwing area to perform the cup throwing operation, then move to the liquid addition level 100, take out the second dilution cup located at the liquid addition level 100, and perform the first dilution cup on the second dilution cup as described above. During the mixing operation, the second dilution is mixed evenly, the movement of the rotating mechanism 20 is controlled, and the subsequently added dilution cup is driven to the liquid addition level 100. After the second dilution cup is mixed, it is moved to the position for subsequent detection. detection. If there are three or more multiple dilution requirements, each component can be driven to complete the above-mentioned first and second dilution steps. All components of the entire dilution process work closely together to achieve high-efficiency multiple dilutions.

Each embodiment of the present application uses the first dilution cup and the second dilution cup when describing the operation or effect of each component, which does not represent a limitation on the use of each component in the device. The first dilution cup and the second dilution cup can also be used. are other dilution cups.

Please refer to FIG. 12 , which is a schematic block diagram of a circuit structure of an embodiment of the sample analyzer of the present application. The sample analyzer 500 includes a sample detection device 501 and a sample dilution device 502, wherein the sample dilution device 502 can be the sample dilution device provided by any of the above-mentioned embodiments of the present application. During the sample analysis process, the sample dilution device 502 passes through at least two. The sample to be tested that meets the detection requirements is prepared by sub-dilution, and the preparation implementation method can refer to the dilution operation flow of each embodiment of the above-mentioned sample dilution device, which will not be repeated; the sample detection device 501 detects the sample to be tested.

The sample dilution device 502 can be applied to any sample analyzer that needs to dilute the sample, such as a blood cell analyzer, a life analyzer, and a blood coagulation analyzer. When the sample dilution device 502 is applied to a coagulation analyzer, the dilution cup can be a sample cup for optical detection; when the sample analyzer can use an optical method and/or a magnetic bead method for detection, the The intermediate transposition can be the sample cup detected by the optical method or the sample cup detected by the magnetic bead method. In order to save costs, when the magnetic bead method is used for detection, before obtaining the target diluent, the dilution cups used can be all optical cups. , and the obtained target diluent is added to the magnetic bead cup for subsequent detection.

Please refer to FIG. 13. The present application also provides a method for detecting a sample. Taking secondary dilution as an example, the method for detecting a sample includes the following steps:

S101: Determine a first dilution ratio and a second dilution ratio.

The first dilution ratio and the second dilution ratio are determined according to the steps of each embodiment of the sample dilution method.

S102: Aspirate the diluent and the sample liquid based on the first dilution ratio, and spit the sucked liquid into the first dilution cup to obtain the first solution.

Wherein, after the first dilution ratio and the second dilution ratio are determined according to step S25, the first sample dosage ρa ₁ and the diluent dosage ρb ₁ for the first dilution can be determined, and the second sample dosage a ₂ for the second dilution can be determined and the amount of diluent b ₂ .

In this step, the sample solution and the diluent can be drawn according to the first sample dosage ρa ₁ and the diluent dosage ρb ₁ respectively. The first dilution cup is the first dilution cup described in the above embodiments.

S103: Aspirate the diluent and the first solution based on the second dilution ratio, and spit the sucked liquid into the second dilution cup to obtain the second solution.

In this step, the first solution and the diluent can be drawn according to the second sample amount a ₂ and the diluent amount b ₂ . The second dilution cup is the second dilution cup described in the above embodiments.

S104: Detect the second solution.

The second solution obtained by dilution in steps S102 to S103 in this embodiment is a solution for detection, and the dilution ratio of the sample in the second solution is the target dilution ratio. The dilution ratio of each dilution step is determined by using each step of the sample dilution method, Errors caused by the sampling accuracy of the sampling needle and the like can be reduced, so that the dilution of the sample is more accurate, and the detection result of the diluted sample is also more accurate.

Referring to FIG. 14, step S102 may include:

S1021: Control the liquid adding mechanism to absorb the dilution liquid and the sample liquid based on the amount of the dilution liquid and the amount of the sample liquid determined by the first dilution ratio.

Among them, the liquid adding mechanism can first move to the container containing the diluent, absorb the diluent, and then move to the container containing the sample liquid, and absorb the sample liquid; The sample liquid is then moved to the container containing the diluent, and the diluent is sucked, and the order of the diluent is determined according to the actual moving path of the liquid addition mechanism, which is not limited here.

S1022: Control the liquid addition mechanism to move to the liquid addition level, and spit the sucked diluent and sample liquid into the first dilution cup located at the liquid addition level to obtain the first solution.

After this step, the liquid adding mechanism can be controlled to move to an area where the cleaning operation can be performed for cleaning, so as to prepare for the next liquid collection.

S1023: Control the transfer component to transfer the first dilution cup to the intermediate transfer position.

After the transfer component can be controlled to take out the first dilution cup, mix the first dilution cup evenly, and then move it to the intermediate transfer position; or, the transfer assembly can be controlled to transfer the first dilution cup to the intermediate transfer position. Cup mix well.

Wherein, taking the transfer assembly 40 of the previous embodiment as an example, controlling the transfer assembly to take out the first dilution cup may include steps a-b:

a: Control the transfer component to move to the liquid level;

b: The motion mechanism 60 is controlled to work, so that the gripper slider 72 moves to the main shaft section 741 to grab the first dilution cup.

Controlling the transfer component to mix the first dilution cup may include steps c-d:

c: Control the movement mechanism 60 to work, so that the gripper slider 72 moves to the secondary shaft section 743;

d: Control the rotating motor 79 to work, so that the rotating shaft 74 rotates, so as to drive the gripper slider 72 to shake, and perform the mixing operation on the first dilution cup.

Controlling the transfer assembly to transfer the first dilution cup to the intermediate position may include: controlling the transfer assembly to move to the intermediate position along a preset path.

After step S1023, the liquid sampling level is vacant, and the second dilution cup can be transferred to the liquid sampling level.

Referring to FIG. 15, step S103 may include:

S1031: Control the liquid adding mechanism to absorb the diluent and the first solution based on the amount of the diluent and the amount of the first solution determined by the second dilution ratio.

In this step, the liquid adding mechanism is controlled to move to the intermediate position and the position of the container containing the diluent, respectively, and the first solution and the diluent are drawn according to the determined second sample dosage a ₂ and the diluent dosage b ₂ respectively, and the liquid adding mechanism can move first. To the container containing the diluent, absorb the diluent, and then move to the middle transfer position to absorb the first solution; it can also be reversed, move to the middle transfer position first, absorb the first solution, and then move to the container containing the diluent , sucking the diluent, and its order is determined according to the actual moving path of the liquid adding mechanism, which is not limited here.

S1032 : control the liquid addition mechanism to move to the liquid addition level, and spit the sucked diluent and the first solution into the second dilution cup located at the liquid addition level to obtain the second solution.

S1033: Control the transfer component to take out the second dilution cup, and perform a mixing operation on the second dilution cup to mix the second solution uniformly.

For this operation, reference may be made to the above steps a to d, which will not be repeated.

S1034: Control the transfer component to transfer the second dilution cup to a detection area to detect the second solution.

In this step, the transfer component is controlled to transfer the second dilution cup to the detection area along a preset path.

So far, the dilution operation of the sample can be completed. The same is true for multiple dilutions. On the basis of the above steps, it is sufficient to control the periodic operation of each component and mechanism, and will not be repeated here.

Please refer to FIG. 16 . FIG. 16 is a schematic block diagram of the circuit structure of an embodiment of the sample detection apparatus of the present application. The sample detection device 31 includes a processor 311 and a memory 312 coupled to each other, the memory 312 stores a computer program, and the processor 311 is configured to execute the computer program to implement the steps of the above embodiments of the sample dilution method of the present application.

Alternatively, the memory 312 stores a computer program of the above-mentioned sample detection method, and the processor 311 is configured to execute the computer program to implement the steps of the above-mentioned embodiments of the sample detection method of the present application.

For the description of each step performed by the processing, please refer to the description of each step in the above-mentioned embodiment of the sample dilution method of the present application, which will not be repeated here.

In various embodiments of the present application, the disclosed sample dilution method and sample detection device may be implemented in other ways. For example, the above-described embodiments of the sample detection apparatus are only illustrative. For example, the division of the modules or units is only a logical function division. In actual implementation, there may be other division methods, such as multiple divisions. Units or components may be combined or may be integrated into another system, or some features may be omitted, or not implemented. On the other hand, the shown or discussed mutual coupling or direct coupling or communication connection may be through some interfaces, indirect coupling or communication connection of devices or units, and may be in electrical, mechanical or other forms.

The units described as separate components may or may not be physically separated, and components displayed as units may or may not be physical units, that is, may be located in one place, or may be distributed to multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution in this implementation manner.

In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically alone, or two or more units may be integrated into one unit. The above-mentioned integrated units may be implemented in the form of hardware, or may be implemented in the form of software functional units.

The integrated unit, if implemented in the form of a software functional unit and sold or used as an independent product, may be stored in a computer-readable storage medium. Based on this understanding, the technical solutions of the present application can be embodied in the form of software products in essence, or the parts that contribute to the prior art, or all or part of the technical solutions, and the computer software products are stored in a storage medium .

Referring to FIG. 17, FIG. 17 is a schematic block diagram of the circuit structure of an embodiment of the computer-readable storage medium of the present application. The computer storage medium 1000 stores a computer program 1001, and when the computer program 1001 is executed, the above-mentioned sample dilution method of the present application and/or The steps of each embodiment of the above-mentioned sample detection method.

The computer storage medium 1000 may be a USB flash drive, a removable hard disk, a read-only memory (ROM, Read-Only Memory), a random access memory (RAM, Random Access Memory), a magnetic disk or an optical disk and other media that can store program codes.

The above are only the embodiments of the present application, and are not intended to limit the scope of the patent of the present application. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present application, or directly or indirectly applied to other related technologies Fields are similarly included within the scope of patent protection of this application.

Claims

A sample dilution method, characterized in that the method comprises:

Estimate at least one dilution ratio;

determining another dilution ratio according to the target dilution ratio of the sample and the at least one dilution ratio;

Adjust the at least one dilution ratio until the other dilution ratio meets a preset requirement, and determine that the current at least one dilution ratio and the other dilution ratio are respectively one of the multiple dilution processes of the sample The dilution ratio used in the dilution process.
The method of claim 1, wherein the other dilution ratio comprises a first dilution ratio, the at least one dilution ratio comprises a second dilution ratio, the first dilution ratio and the second dilution ratio are the dilution ratios used in one of the two dilution processes of the sample, respectively.
The method according to claim 2, wherein the first dilution ratio is a dilution ratio used in a first dilution process of the sample, and the second dilution ratio is a second dilution of the sample The dilution ratio used in the process;

The estimated at least one dilution ratio includes:

Estimated second dilution ratio;

The determining another dilution ratio according to the target dilution ratio of the sample and the at least one dilution ratio, including:

determining the first dilution ratio according to the target dilution ratio of the sample and the second dilution ratio;

The adjusting of the at least one dilution ratio until the other dilution ratio meets a preset requirement includes:

The second dilution ratio is adjusted until the first dilution ratio meets the preset requirement.
The method according to claim 3, wherein the method further comprises:

Obtain:

the volume of the target solution;

The range of solution volumes for the first dilution process; and

The proportional relationship between the volume of the first sample used in the first dilution process and the volume of the second sample used in the second dilution process; wherein the second sample is a diluted solution from the first sample obtained in;

The preset requirement is determined by using the volume of the target solution, the solution volume range, the first dilution ratio, the target dilution ratio, and the proportional relationship.
The method according to claim 3, wherein the adjusting the second dilution ratio until the first dilution ratio meets a preset requirement comprises:

When the first dilution ratio does not meet the preset requirement, adjust the second dilution ratio with a preset adjustment step;

The step of determining the first dilution ratio according to the target dilution ratio of the sample and the second dilution ratio is repeated until the first dilution ratio meets a preset requirement.
The method of claim 5, wherein the estimating the second dilution ratio comprises:

Taking 0.5 as the initial value of the second dilution ratio;

The adjusting the second dilution ratio until the first dilution ratio meets the preset requirements, further comprising:

The second dilution ratio is increased or decreased by taking 0.1 as the preset adjustment step size until the first dilution ratio meets the preset requirement.
The method according to claim 3, wherein the determining the first dilution ratio according to the target dilution ratio of the sample and the second dilution ratio comprises:

The first dilution ratio is calculated using the following formula:

γ=γ 1 ·γ 2

Wherein, γ is the target dilution ratio, γ 1 is the first dilution ratio, and γ 2 is the second dilution ratio.
A sample detection device, characterized in that, the sample detection device comprises a processor and a memory coupled to each other; a computer program is stored in the memory, and the processor is used to execute the computer program to realize the method as claimed in claim 1 The steps of any one of -7.
A computer-readable storage medium, characterized in that the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, implements the steps of the method according to any one of claims 1-7.
A sample dilution device, characterized in that it includes:

Add liquid level, used to load the first dilution cup;

a liquid addition mechanism, arranged above the liquid addition level, for sucking the diluent and the sample liquid, and adding them to the first dilution cup located at the liquid addition level to form a primary dilution sample liquid;

a middle transfer position, arranged below the liquid adding mechanism, for receiving the first dilution cup containing the primary dilution sample liquid;

The liquid adding mechanism is also used for sucking the diluent and the primary dilution sample liquid and adding them into the second dilution cup to form the secondary dilution sample liquid.
The sample dilution device according to claim 10, characterized in that, the sample dilution device further comprises a rotation mechanism, and the rotation mechanism comprises a fixed seat and a rotation device provided in the fixed seat and rotatably arranged relative to the fixed seat The outer edge of the rotary seat is provided with a plurality of dilution cup holes, and the dilution cup holes will pass the liquid filling level when the rotary seat rotates.
The sample dilution device according to claim 11, wherein the hole of the dilution cup passes through the liquid addition level and the intermediate transfer level in sequence when the hole of the dilution cup rotates with the rotating seat.
The sample diluting device according to claim 11, wherein the sample diluting device further comprises a cup-carrying table, the rotating mechanism is embedded in the cup-carrying table, and the intermediate position is recessed in the fixing seat The counterbore on the surface, or the intermediate index is a counterbore recessed on the surface of the cup-carrying table.
The sample dilution device according to claim 13, wherein the counterbore is a round hole or a square hole, and the depth of the counterbore is smaller than the height of the first dilution cup or the second dilution cup.
The sample dilution device according to claim 11, wherein the sample dilution device further comprises a transfer assembly disposed above the rotating mechanism, the transfer assembly comprising a motion mechanism and a mixing mechanism, and the motion mechanism uses In order to enable the mixing mechanism to grip and transfer the first dilution cup at the liquid addition level, the mixing mechanism is used for mixing the first dilution cup during the gripping and transferring process.
The sample dilution device according to claim 15, wherein the liquid adding mechanism uses the mixing mechanism as the intermediate position and directly sucks the primary dilution sample liquid on the mixing mechanism.
The sample dilution device according to claim 10, wherein when the first dilution cup or the second dilution cup is located at the liquid addition position or the intermediate transfer position, the first dilution cup or the The cup mouth end of the second dilution cup is arranged in a protruding shape so as to be clamped.
A sample detection method, characterized in that the method comprises:

Determine the first dilution ratio and the second dilution ratio according to the method of any one of claims 1-7;

Aspirate the diluent and the sample liquid based on the first dilution ratio, and spit the sucked liquid into the first dilution cup to obtain the first solution;

Draw the diluent and the first solution based on the second dilution ratio, and spit the drawn liquid into the second dilution cup to obtain the second solution;

The second solution is tested.
The method according to claim 18, wherein the drawing the diluent and the sample liquid based on the first dilution ratio, and spitting the drawn liquid into the first dilution cup to obtain the first solution comprises:

controlling the liquid adding mechanism to draw the diluent and the sample liquid based on the amount of the diluent and the sample liquid determined by the first dilution ratio;

controlling the liquid addition mechanism to move to the liquid addition level, and spitting the sucked diluent and the sample liquid into the first dilution cup located at the liquid addition level to obtain the first solution;

The control transfer assembly transfers the first dilution cup to the intermediate transfer position.
The method according to claim 18, wherein the drawing of the diluent and the first solution based on the second dilution ratio, and spitting the drawn liquid into the second dilution cup to obtain the second solution comprises:

Control the liquid adding mechanism to absorb the diluent and the first solution based on the amount of the diluent and the amount of the first solution determined by the second dilution ratio;

Controlling the liquid addition mechanism to move to the liquid addition level, and spitting the sucked diluent and the first solution into the second dilution cup located at the liquid addition level to obtain the second solution;

Controlling the transfer assembly to take out the second dilution cup, and performing a mixing operation on the second dilution cup to mix the second solution uniformly;

The transfer assembly is controlled to transfer the second dilution cup to a detection area to detect the second solution.