CN111089955A - Substance concentration determination method, sample analyzer and storage medium - Google Patents
Substance concentration determination method, sample analyzer and storage medium Download PDFInfo
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- CN111089955A CN111089955A CN201811244159.2A CN201811244159A CN111089955A CN 111089955 A CN111089955 A CN 111089955A CN 201811244159 A CN201811244159 A CN 201811244159A CN 111089955 A CN111089955 A CN 111089955A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
Abstract
The embodiment of the application discloses a method for determining substance concentration, a sample analyzer and a storage medium, which comprise the following steps: measuring a sample to obtain a first concentration value; determining a final concentration value from the first concentration value if the first concentration value is within a first threshold range; if the first concentration value exceeds the first threshold range and is within the second threshold range, diluting or concentrating the sample, then measuring the sample again and obtaining a second concentration value according to the measured value and the dilution or concentration ratio; determining a final concentration value according to the second concentration value if the second concentration value is within a third threshold range; determining a final concentration value according to the first concentration value if the second concentration value exceeds a third threshold range; and if the first concentration value exceeds the first threshold range and exceeds the second threshold range, diluting or concentrating the sample, then measuring the sample again, obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining the final concentration value according to the second concentration value.
Description
Technical Field
The application relates to the field of medical treatment and detection, and relates to but is not limited to a method for determining substance concentration, a sample analyzer and a storage medium.
Background
Among clinical items of a sample analyzer, there are items related to measuring concentration. For example, in a blood coagulation analyzer, fibrinogen (Fib) measurement, D-dimer measurement, and Fibrin Degradation Products (FDP) measurement are required, and the measurement range actually required in the clinical field is wide, but the linear range of the reagent itself does not reach the clinical measurement range, in which case, in the related art: if the measured concentration exceeds the measurement range declared by the reagent, the instrument makes the diluted or concentrated sample fall into the measurement range declared by the reagent again by diluting or concentrating the measured sample, obtains the initial measured concentration by inquiring the calibration curve, and then recalculates the final reported concentration according to the dilution or concentration ratio. However, this method often brings about a problem that: at the concentration point near the dilution threshold or the concentration threshold set by the instrument, due to the system repeatability, sometimes the dilution or concentration retest is triggered, sometimes the dilution or concentration retest is not triggered, and the two measurement results have an error, so that the error is mainly caused by the matrix effect.
When a customer measures a sample, if the result is abnormal, the measurement is re-performed on the instrument. For example, if the dilution threshold point is 5, the first measurement is 4.9, no dilution retest is initiated, the customer re-measures, and the second measurement is 5.2, the dilution threshold is exceeded, and the system will initiate a dilution retest, then the dilution retest result may be 6, which may be annoying to the customer and may not determine which value to report finally, resulting in an inaccurate measurement.
Disclosure of Invention
In view of the above, embodiments of the present application provide a method for determining a concentration of a substance, a sample analyzer, and a storage medium to solve at least one problem in the related art.
The technical scheme of the embodiment of the application is realized as follows:
in a first aspect, an embodiment of the present application provides a method for determining a concentration of a substance, which is applied to a sample analyzer, and includes:
measuring a sample to obtain a first concentration value;
determining a final concentration value from the first concentration value if the first concentration value is within a first threshold range;
if the first concentration value exceeds the first threshold range and is within the second threshold range, diluting or concentrating the sample, then measuring the sample again and obtaining a second concentration value according to the measured value and the dilution or concentration ratio; determining a final concentration value according to the second concentration value if the second concentration value is within a third threshold range; determining a final concentration value according to the first concentration value if the second concentration value exceeds a third threshold range;
and if the first concentration value exceeds the first threshold range and exceeds the second threshold range, diluting or concentrating the sample, then measuring the sample again, obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining the final concentration value according to the second concentration value.
In a second aspect, an embodiment of the present application provides a sample analyzer, including: a detector, a processor and a controller;
the detector is used for detecting the substance concentration of the sample;
the processor is used for processing the detection data to obtain a detection result;
the controller is used for diluting or concentrating the sample;
wherein the detector measures the sample to obtain a first concentration value;
determining, by the processor, a final concentration value from the first concentration value if the first concentration value is within a first threshold range;
if the first concentration value is beyond the first threshold range but within the second threshold range, the controller dilutes or concentrates the sample, and then the detector measures the sample again and obtains a second concentration value according to the measured value and the dilution or concentration ratio; determining, by the processor, a final concentration value from the second concentration value if the second concentration value is within a third threshold range; if the second concentration value exceeds a third threshold range, the processor determines a final concentration value according to the first concentration value;
if the first concentration value exceeds a first threshold range and exceeds a second threshold range, the controller dilutes or concentrates the sample, then the detector measures the sample again and obtains a second concentration value according to the measured value and the dilution or concentration ratio, and the processor determines a final concentration value according to the second concentration value.
In a third aspect, an embodiment of the present application provides a storage medium, in which a program is stored, and the program, when executed by a processor, implements the steps of the method for determining a substance concentration as described above.
The embodiment of the application provides a method for determining substance concentration, a sample analyzer and a storage medium, wherein whether a first concentration value of a measured sample is within a first threshold range is judged firstly, if the first concentration value of the measured sample is not within the first threshold range, dilution or concentration is carried out, and if the result of dilution retest does not exceed a defined second threshold range, the result of dilution retest is reported; if the dilution retest result exceeds the limited second threshold range, the first concentration threshold is reported, so that whether dilution or concentration retest is needed or not is judged through a plurality of threshold ranges, a more accurate measurement result is intelligently selected for a user, and the user experience is improved.
Drawings
FIG. 1 is a schematic flow chart of a method for determining a concentration of a substance according to an embodiment of the present disclosure;
FIG. 2 is a schematic flow chart of another implementation of the method for determining a substance concentration according to the embodiment of the present application;
FIG. 3 is a graph of a fitted curve of the true concentration value compared to a reference value using a correlation technique;
FIG. 4 is a schematic flow chart illustrating an implementation of diluting a sample by a method for determining a concentration of a substance according to an embodiment of the present disclosure;
FIG. 5 is a schematic flow chart illustrating the implementation of the method for concentrating a sample according to the embodiment of the present application;
FIG. 6 is a schematic diagram of the composition of a sample analyzer according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application.
The present application provides a method for determining a substance concentration, which is applied to a sample analyzer, such as a blood cell analyzer, a biochemical immunoassay analyzer, a blood coagulation analyzer, and other in vitro diagnostic apparatuses. Fig. 1 is a schematic flow chart of an implementation of a method for determining a substance concentration according to an embodiment of the present application, as shown in fig. 1, the method includes the following steps:
step S101, a sample is measured to obtain a first concentration value.
Here, the sample may be a blood sample, such as plasma. Measurements may be made of specific substances in the sample, such as fibrinogen, D-dimer, FDP, etc.
Step S102, if the first concentration value is within the first threshold range, determining a final concentration value according to the first concentration value. For example, the first concentration value may be directly determined as the final concentration value.
Here, the first threshold range may be determined based on historical measurement data, or may be a predetermined value. The concentration of the liquid to be diluted and retested is beyond the threshold range; there is also a threshold range for which concentration retesting is required, i.e., concentration exceeding the threshold range requires concentration retesting. For those requiring dilution retesting, the first threshold range is less than the dilution threshold D1. For those requiring a concentration retest, the first threshold range is greater than a concentration threshold D2.
Thus, the first threshold range may be a set dilution threshold D1 and/or a concentration threshold D2; the final concentration value is a measurement result finally obtained by the sample analyzer. The step S102 may be understood as that, when the first concentration value X1 is smaller than D1, the first concentration value is taken as a final concentration value, i.e., a measurement result.
If the first density value is out of the first threshold range, step S103 or step S106 is performed.
Step S103, if the first concentration value is beyond the first threshold range but within the second threshold range, diluting or concentrating the sample, then measuring the sample again and obtaining a second concentration value according to the measured value and the dilution or concentration ratio.
Here, the second threshold range is related to the first threshold range. For example, the first threshold range may be smaller than the dilution threshold D1, the second threshold range may be (D1, D1+ a1), a1 is a preset value, if the first concentration value X1 is larger than the dilution threshold D1, but within the second threshold range (D1, D1+ a1), it indicates that the concentration of the sample is too large, the sample is diluted, and the sample is measured again and the second concentration value is obtained according to the measured value and the dilution or concentration ratio; for another example, the first threshold range may be greater than the concentration threshold D2, the second threshold range may be (D2-a2, D2), a2 is a preset value, if the first concentration value X3 is less than the concentration threshold D2, but within the second threshold range (D2-a2, D2), it is indicated that the concentration of the sample is too small, the sample is concentrated, and the sample is measured again and the second concentration value is obtained according to the measured value and the dilution or concentration ratio.
After the second concentration value is obtained, step S104 or step S105 is performed according to the second concentration value.
Step S104, if the second concentration value is within the third threshold value range, determining a final concentration value according to the second concentration value. For example, the second concentration value may be directly determined as the final concentration value.
Here, the third threshold range is related to the first threshold range. For example, the first threshold range may be smaller than the dilution threshold D1, the third threshold range may be (D1-B1, D1+ B1), B1 is a preset value, if the sample needs to be diluted, a second concentration value obtained by diluting the sample is measured, and if the second concentration value is within the third threshold range (D1-B1, D1+ B1), a final concentration value is obtained according to the second concentration value and the dilution ratio; for another example, the first threshold range may be greater than the concentration threshold D2, the third threshold range may be (D2-B2, D2+ B2), B2 is a preset value, if the sample needs to be concentrated, a second concentration value obtained by concentrating the sample is measured, and if the second concentration value is within the third threshold range (D2-B2, D2+ B2), a final concentration value is obtained according to the second concentration value and the concentration ratio.
Step S105, if the second concentration value exceeds the third threshold range, determining a final concentration value according to the first concentration value. For example, the first concentration value may be directly determined as the final concentration value.
Here, if the sample needs to be diluted, a second concentration value obtained by diluting the sample is measured, and if the second concentration value is not within a third threshold range (D1-B1, D1+ B1), the first concentration value X1 is reported to the user as a final concentration value; if the sample needs to be concentrated, a second concentration value obtained by the sample after the sample is concentrated is measured, and if the second concentration value is not within the third threshold range (D2-B2, D2+ B2), the first concentration value X3 can be output to the user as a final concentration value.
Step S106, if the first concentration value exceeds the first threshold range and exceeds the second threshold range, diluting or concentrating the sample, then measuring the sample again, obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining the final concentration value according to the second concentration value. For example, the second concentration value may be directly determined as the final concentration value.
Here, if the first concentration value X1 is greater than D1+ a1, that is, exceeds the second threshold range (D1, D1+ a1), the sample is diluted to obtain a second concentration value, and then a final concentration value is obtained from the second concentration value and the dilution ratio. If the first concentration value X3 is smaller than D2-A2, namely the second threshold range is not reached (D2-A2, D2), the sample is concentrated to obtain a second concentration value, and then a final concentration value is obtained according to the second concentration value and the concentration.
For the determination of the first threshold range in step S102, the determination may also be based on historical measurement data in the present embodiment. For example, in the case where the sample needs to be diluted, if the concentration value determined at the end of the threshold range (D1, D1+ c1) is determined from the first concentration value (where c1 is a number smaller than a1) and the concentration value determined at the end of the threshold range (D1+ c1, D1+ a1) is determined from the second concentration value after the same kind of reagent is used for a large amount of measurement by using the methods corresponding to steps S101 to S106, the concentration point of D1+ c1 is determined as the dilution threshold and smaller than D1+ c1 becomes the first threshold range, and thus the first threshold range, i.e., the threshold point of the post-dilution, can be slightly delayed, thereby improving the measurement efficiency.
In the embodiment, the first concentration value which is the first measurement result is judged, if the first concentration value is too large or too small, the sample is diluted or concentrated, then measurement is carried out again, the second concentration value is judged, and finally the final concentration value is obtained according to the judgment result, so that the threshold value point is expanded to be a threshold value range, a more accurate measurement result is intelligently selected for a user, and the user experience is improved.
The method for determining the substance concentration intelligently determines the final reported value, and by taking dilution retest as an example, the reported result is closer to the value D1 in the range (D1, D1+ A1) near D1, namely, the reported result is more accurate. However, in the conventional method, the reported value is determined by the instrument according to the D1 threshold directly, and the reported value in the range (D1, D1+ a1) near D1 may deviate from the D1 value more than the method, which is not accurate.
In practice, the user may be provided with two modes, if the first mode is selected, the instrument performs a dilution or concentration re-measurement scheme that extends the threshold point to a threshold range (as described in steps S202-S207 below), and if the second mode is selected, the instrument performs a conventional dilution or concentration re-measurement scheme (as described in steps S208-S210 below).
An embodiment of the present application provides a method for determining a substance concentration, which is applied to a sample analyzer, and fig. 2 is a schematic flow chart of another implementation of the method for determining a substance concentration according to the embodiment of the present application, as shown in fig. 2, the method includes the following steps:
step S201, providing an interface to receive an instruction input by a user, and if the received instruction is a first target instruction, acquiring an intermediate parameter from a measurement sample.
Here, when the sample analyzer is a coagulation analyzer, the intermediate parameter includes light flux data or coagulation time. If the received first target instruction is the first target instruction, the scheme corresponding to the step S202 to the step S207 is executed. For example, the machine enters the first mode after receiving the first target instruction. In this embodiment, the sample analyzer may also be a biochemical immunoassay analyzer.
And S202, determining the concentration of the substance according to the intermediate parameter and a preset corresponding relation.
Here, the preset correspondence is a correspondence between the intermediate parameter and the concentration. For example, in general, the measurement result of the instrument system is not the concentration dimension, but the light flux value (e.g., D-dimer) or the coagulation time (e.g., Fib), but the measurement result is the concentration of the sample to be measured, and the final concentration of the substance can be determined according to the intermediate parameter (e.g., the light flux value (or the converted optical density od value) or the coagulation time) measured by the instrument system by using the preset corresponding relationship (e.g., calibration curve).
Step S203, if the first concentration value is within the first threshold value range, determining a final concentration value according to the first concentration value.
Step S204, if the first concentration value is beyond the first threshold range but within the second threshold range, the sample is diluted or concentrated, and then the sample is measured again to obtain a second concentration value according to the measured value and the proportion of dilution or concentration.
In step S205, if the second concentration value is within the third threshold value range, a final concentration value is determined according to the second concentration value.
In step S206, if the second concentration value exceeds the third threshold range, a final concentration value is determined from the first concentration value.
Step S207, if the first concentration value exceeds the first threshold range and exceeds the second threshold range, diluting or concentrating the sample, then measuring the sample again and obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining a final concentration value according to the second concentration value.
If the received second target instruction is the second target instruction, the scheme corresponding to the step S208 to the step S210 is executed. For example, the machine enters the second mode after receiving the second target instruction.
Step S208, measuring the sample to obtain a first concentration value.
In step S209, if the first concentration value is within the first threshold range, a final concentration value is determined from the first concentration value.
Step S210, if the first concentration value exceeds the first threshold range, diluting or concentrating the sample, then measuring the sample again, obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining a final concentration value according to the second concentration value.
For example, the first threshold range may be smaller than the dilution threshold D1, if the first concentration value X1 is greater than the dilution threshold D1, the sample is diluted and then measured again, and the final concentration value is obtained according to the obtained second concentration value and the corresponding dilution ratio; for another example, the first threshold range may be greater than the concentration threshold D2, if the first concentration value X3 is less than the concentration threshold D2, the sample is concentrated, and then the measurement is performed again, and according to the obtained second concentration value and the corresponding concentration ratio, the final concentration value is obtained.
In this embodiment, when a first target instruction input by a user is received, the instrument executes a dilution re-measurement or concentration re-measurement scheme that expands a threshold point into a threshold range; if the user inputs the second target instruction, the instrument executes a conventional dilution re-measurement or concentration re-measurement scheme; therefore, the working mode of the sample analyzer is determined according to the selection of the user, the user experience is improved, and the method is more humanized.
In the related art, if the measured concentration exceeds the measurement range declared by the reagent, the instrument makes the diluted or concentrated sample fall into the measurement range declared by the reagent again by diluting or concentrating the sample, obtains the initial measured concentration by inquiring a calibration curve, and then recalculates the final concentration according to the dilution or concentration ratio; with the method in the related art, a large number of samples are measured at random, and if the total final measurement result, i.e., the reported value, is compared with the reference value, a fault occurs near the dilution threshold point or the concentration threshold set by the system. As shown in fig. 3, a curve 301 represents a fitting curve between a reference value and an actual final measurement result, i.e., a reported value, and it can be seen from the curve 301 that, in the related art, because both a dilution threshold or a concentration threshold are only a specific value, near the threshold point, due to the system repeatability, a dilution retest or a concentration retest may be started, and a dilution retest or a concentration retest may not be started, so that a difference between the obtained final measurement result, i.e., the reported value and the reference value, is relatively large, and a fault of the curve 301 at the dilution threshold or the concentration threshold is formed.
The embodiment of the application provides a method for determining the concentration of a substance for avoiding the occurrence of a fault phenomenon in a dilution threshold or a concentration threshold, and when a sample analyzer measures a certain parameter, because the measurement range of a reagent per se is possibly relatively narrow, two concentration points can be preset in the sample analyzer, one is that the dilution threshold point is used for judging whether dilution is needed or not, and the other is that the concentration threshold point is used for judging whether concentration is needed or not.
For example, the first threshold range is smaller than the dilution threshold D1, and the process of performing dilution and re-measurement on the sample in this embodiment is as follows:
when the first concentration value is higher than the dilution threshold D1, the instrument processes, i.e. dilutes, the sample, e.g. by using a diluent or other serum substances, the dilution ratio may be 2 times or other multiples, so that the diluted measured concentration theoretically falls within the measurement range of the reagent itself, then re-measures the diluted sample, and since the measured result (i.e. the second concentration value) is the result after dilution, the result needs to be multiplied by the original dilution multiple again, which is called as the dilution re-measurement result, and finally reports the final concentration value according to the judgment, so that the measurement range of the system is extended upward. In this embodiment, the sample analyzer may be a coagulation analyzer or other in vitro diagnostic instrument, such as a chemiluminescence analyzer.
If the first test value (i.e. the first concentration value) is X1, if the first concentration value X1 of the first test sample is within the second threshold range (D1, D1+ a1), the instrument needs to start the dilution retest, and the result of the dilution retest, i.e. the second concentration value, is X2, but additional judgment and processing are needed, namely, if the result of the dilution retest, X2, is within (D1-B1, D1+ B1), the result X2 is determined as the final concentration value, and if the result of the dilution retest, X2, is not within (D1-B1, D1+ B1), the result X1 is determined as the final concentration value. Here a1 may be equal to B1. If the first concentration value X1 of the first test sample is greater than D1+ a1, a conventional dilution retest is performed and the result X2 is determined as the final concentration value. As shown in fig. 4, the method can be implemented by the following steps:
step S401, the sample analyzer is started.
Step S402, measure the sample to obtain a first concentration value X1, and determine whether the first concentration value X1 is greater than a dilution threshold D1.
Here, if the first concentration value X1 is smaller than the dilution threshold value D1 (i.e., within the first threshold value range), step S407 is entered; if the first concentration value X1 is greater than the dilution threshold value D1 (i.e., exceeds the first threshold range), the flow proceeds to step S403.
In step S403, if the first concentration value X1 is greater than the dilution threshold D1, the sample is diluted and retested to obtain a second concentration value X2.
In step S404, it is determined whether the first density value X1 is within the second threshold range (D1, D1+ a 1).
Here, if the first density value X1 is within the second threshold range (D1, D1+ a1), proceed to step S405; the first density value X1 is not within the second threshold range (D1, D1+ a1), and the process advances to step S406.
In step S405, it is determined whether the second concentration value X2 is within the third threshold range (D1-B1, D1+ B1).
Here, if the second density value X2 is within the third threshold range (D1-B1, D1+ B1), proceed to step S406; if the second concentration value X2 is not within the third threshold range (D1-B1, D1+ B1), proceed to step S407.
In step S406, the dilution ratio corresponding to the first concentration value X1 is multiplied by the second concentration value X2 to obtain a final concentration value.
In step S407, the first density value X1 is set as a final density value.
Similarly, in the embodiment of the present application, the first threshold range is greater than the concentration threshold D2, and the concentration retest for the sample in this embodiment is as follows:
when the first concentration value is lower than the concentration threshold, the instrument processes the sample, specifically, performs concentration, for example, concentration by using diluent or other serum substances, which can change the proportional relationship between the sample and the diluent or the requirement in the reagent itself to perform concentration, for example, the proportional relationship is increased by 2 times or other times, so that the measured concentration after concentration theoretically falls within the measurement range of the reagent itself, and then performs measurement again on the concentrated sample, and the result after measurement needs to be divided by the multiple of concentration or other times again because the result is the result after concentration, and then reports the result as the final result, which is called the result of concentration re-measurement, so that the measurement range of the system is extended downward.
If the first test value is X3, if the first concentration value X3 of the first test sample is in the interval (D2-A2, D2), the instrument needs to start concentration retest, the result of the concentration retest is X4, but additional judgment and processing are needed, namely, if the result X4 of the concentration retest is in (D2-B2, D2+ B2), the result X4 is reported, and if the result X4 of the concentration retest is not in (D2-B2, D2+ B2), the result X3 is reported. Here a2 may be equal to B2. And if the first test value is less than D2-A2, carrying out conventional concentration retest, and reporting the result of the concentration retest X3. As shown in fig. 5, the method can be implemented by the following steps:
step S501, the sample analyzer is started.
Step S502, a sample is measured to obtain a first concentration value X3, and it is determined whether the first concentration value X3 is smaller than a concentration threshold D2.
Here, if the first density value X3 is greater than the thickening threshold D2 (i.e., within the first threshold range), step S507 is entered; if the first concentration value X3 is smaller than the concentration threshold value D2 (i.e., exceeds the first threshold range), the flow proceeds to step S503.
In step S503, if the first concentration value X3 is smaller than the concentration threshold D2, the sample is concentrated and retested to obtain a second concentration value X4.
In step S504, it is determined whether the first density value X3 is within the second threshold range (D2-A2, D2).
Here, if the first density value X3 is within the second threshold range (D2-a2, D2), proceed to step S505; the first density value X1 is not within the second threshold range (D2-a2, D2), and the process proceeds to step S506.
In step S505, it is determined whether the second density value X4 is within the third threshold range (D2-B2, D2+ B2).
Here, if the second density value X4 is within the third threshold range (D2-B2, D2+ B2), proceed to step S506; if the second concentration value X4 is not within the third threshold range (D2-B2, D2+ B2), proceed to step S507.
In step S506, the second density value X4 is divided by the concentration ratio corresponding to the first density value X3 to obtain a final density value.
In step S507, the first density value X3 is set as a final density value.
An embodiment of the present application provides a sample analyzer, fig. 6 is a schematic composition diagram of the sample analyzer in the embodiment of the present application, and as shown in fig. 6, the sample analyzer 600 includes: a detector 601, a processor 602, and a controller 603;
the detector 601 is used for detecting the substance concentration of the sample;
the processor 602 is configured to process the detection data to obtain a detection result;
the controller 603 is configured to dilute or concentrate the sample;
wherein the detector 601 measures the sample to obtain a first concentration value;
determining, by the processor, a final concentration value from the first concentration value if the first concentration value is within a first threshold range;
if the first concentration value is beyond the first threshold range but within the second threshold range, the controller dilutes or concentrates the sample, and then the detector measures the sample again and obtains a second concentration value according to the measured value and the dilution or concentration ratio; determining, by the processor, a final concentration value from the second concentration value if the second concentration value is within a third threshold range; if the second concentration value exceeds a third threshold range, the processor determines a final concentration value according to the first concentration value;
if the first concentration value exceeds a first threshold range and exceeds a second threshold range, the controller dilutes or concentrates the sample, then the detector measures the sample again and obtains a second concentration value according to the measured value and the dilution or concentration ratio, and the processor determines a final concentration value according to the second concentration value.
In other embodiments, the first threshold range is less than D1, the second threshold range is (D1, D1+ a1), the third threshold range is (D1-B1, D1+ B1);
the controller dilutes the sample if the first concentration value is outside a first threshold range.
In other embodiments, the first threshold range is greater than D2, the second threshold range is (D2-A2, D2), the third threshold range is (D2-B2, D2+ B2); the controller concentrates the sample if the first concentration value is outside a first threshold range.
In other embodiments, the first threshold range is determined by the processor from historical measurement data.
In other embodiments, the second threshold range and/or the third threshold range is related to the first threshold range.
In other embodiments, the detector 601 is further configured to:
measuring a sample to obtain an intermediate parameter;
determining the substance concentration according to the intermediate parameter and a preset corresponding relation; the preset correspondence is a correspondence between the intermediate parameter and the concentration.
In other embodiments, the intermediate parameter comprises light flux data or a coagulation time.
In other embodiments, the sample analyzer comprises a coagulation analyzer or a biochemical immunoassay analyzer.
The blood coagulation analyzer according to any one of the above embodiments, which is used for optically measuring and analyzing the amount of a specific substance related to the blood coagulation/fibrinolysis function and the activity level thereof, wherein the sample is blood plasma. The blood coagulation analyzer of the present embodiment optically measures a sample by a coagulation time method, a chromogenic substrate method, and an immunoturbidimetric method. The coagulation time method used in the present embodiment is a measurement method for detecting the coagulation process of a sample as a change in transmitted light. The measurement items include Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen amount (FIB), and the like. Examples of the measurement items of the chromogenic substrate method include Antithrombin-III (AT-III), and the like, and examples of the measurement items of the immunoturbidimetric method include D-Dimer (D-Dimer) and fibrinogen Degradation Products (FDP, Fibrin (-ogen) Degradation Products), and the like.
It should be noted that, if the determination method of the substance concentration is implemented in the form of a software functional module and sold or used as a separate product, it may also be stored in a computer readable storage medium. Based on such understanding, the technical solutions of the embodiments of the present invention may be essentially implemented or a part contributing to the prior art may be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the methods described in the embodiments of the present invention. And the aforementioned storage medium includes: various media capable of storing program codes, such as a usb disk, a removable hard disk, a Read Only Memory (ROM), a magnetic disk, or an optical disk. Thus, embodiments of the invention are not limited to any specific combination of hardware and software.
Accordingly, an embodiment of the present invention further provides a computer storage medium, on which computer-executable instructions are stored, and when the computer-executable instructions are executed by a processor, the steps of the method for determining the concentration of a substance provided by the above-mentioned embodiment are implemented.
The above description of the sample analysis system and computer storage medium embodiments is similar to the description of the method embodiments above, with similar beneficial results as the method embodiments. For technical details not disclosed in embodiments of the sample analysis system and computer storage medium of the present invention, reference is made to the description of embodiments of the method of the present invention for understanding.
It should be appreciated that reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present application. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. It should be understood that, in the various embodiments of the present application, the sequence numbers of the above-mentioned processes do not mean the execution sequence, and the execution sequence of each process should be determined by its function and inherent logic, and should not constitute any limitation to the implementation process of the embodiments of the present application. The above-mentioned serial numbers of the embodiments of the present application are merely for description and do not represent the merits of the embodiments.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
In the several embodiments provided in the present application, it should be understood that the disclosed apparatus and method may be implemented in other ways. The above-described device embodiments are merely illustrative, for example, the division of the unit is only a logical functional division, and there may be other division ways in actual implementation, such as: multiple units or components may be combined, or may be integrated into another system, or some features may be omitted, or not implemented. In addition, the coupling, direct coupling or communication connection between the components shown or discussed may be through some interfaces, and the indirect coupling or communication connection between the devices or units may be electrical, mechanical or other forms.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units; can be located in one place or distributed on a plurality of network units; some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiments of the present application.
In addition, all functional units in the embodiments of the present application may be integrated into one processing unit, or each unit may be separately regarded as one unit, or two or more units may be integrated into one unit; the integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional unit.
Those of ordinary skill in the art will understand that: all or part of the steps for realizing the method embodiments can be completed by hardware related to program instructions, the program can be stored in a computer readable storage medium, and the program executes the steps comprising the method embodiments when executed; and the aforementioned storage medium includes: various media that can store program codes, such as a removable Memory device, a Read Only Memory (ROM), a magnetic disk, or an optical disk.
Alternatively, the integrated units described above in the present application may be stored in a computer-readable storage medium if they are implemented in the form of software functional modules and sold or used as independent products. Based on such understanding, the technical solutions of the embodiments of the present application may be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for causing a terminal to execute all or part of the methods described in the embodiments of the present application. And the aforementioned storage medium includes: a removable storage device, a ROM, a magnetic or optical disk, or other various media that can store program code.
The above description is only for the embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of changes or substitutions within the technical scope of the present application, and all such changes or substitutions should be covered by the present application.
Claims (19)
1. A method for determining a concentration of a substance, applied to a sample analyzer, comprising:
measuring a sample to obtain a first concentration value;
determining a final concentration value from the first concentration value if the first concentration value is within a first threshold range;
if the first concentration value exceeds the first threshold range and is within the second threshold range, diluting or concentrating the sample, then measuring the sample again and obtaining a second concentration value according to the measured value and the dilution or concentration ratio; determining a final concentration value according to the second concentration value if the second concentration value is within a third threshold range; determining a final concentration value according to the first concentration value if the second concentration value exceeds a third threshold range;
and if the first concentration value exceeds the first threshold range and exceeds the second threshold range, diluting or concentrating the sample, then measuring the sample again, obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining the final concentration value according to the second concentration value.
2. The method of claim 1, wherein the first threshold range is less than a dilution threshold D1, the second threshold range is (D1, D1+ a1), the third threshold range is (D1-B1, D1+ B1); the sample is diluted if the first concentration value is outside a first threshold range.
3. The method of claim 1, wherein the first threshold range is greater than a concentration threshold D2, the second threshold range is (D2-a2, D2), the third threshold range is (D2-B2, D2+ B2); the sample is concentrated if the first concentration value is outside a first threshold range.
4. The method of claim 1, wherein the first threshold range is determined based on historical measurement data.
5. The method of claim 1, wherein the second threshold range and/or the third threshold range is related to the first threshold range.
6. The method of claim 1, wherein prior to the measuring the sample to obtain the first concentration value, the method further comprises:
and providing an interface to receive an instruction input by a user, and if the received instruction is a first target instruction, executing subsequent steps according to the first target instruction.
7. The method of claim 6, further comprising:
providing an interface to receive an instruction input by a user, and if the instruction is a second target instruction, executing the following steps according to the second target instruction:
measuring a sample to obtain a first concentration value;
determining a final concentration value from the first concentration value if the first concentration value is within a first threshold range; and if the first concentration value exceeds the first threshold range, diluting or concentrating the sample, then measuring the sample again, obtaining a second concentration value according to the measured value and the dilution or concentration ratio, and determining the final concentration value according to the second concentration value.
8. The method of claim 1, wherein measuring the sample comprises:
obtaining intermediate parameters from the measurement samples;
determining the substance concentration according to the intermediate parameter and a preset corresponding relation; the preset correspondence is a correspondence between the intermediate parameter and the concentration.
9. The method of claim 8, wherein the intermediate parameter comprises light flux data or clotting time.
10. The method of claim 1, wherein the sample analyzer comprises a coagulation analyzer or a biochemical immunoassay analyzer.
11. A sample analyzer, comprising: a detector, a processor and a controller;
the detector is used for detecting the substance concentration of the sample;
the processor is used for processing the detection data to obtain a detection result;
the controller is used for diluting or concentrating the sample;
wherein the detector measures the sample to obtain a first concentration value;
determining, by the processor, a final concentration value from the first concentration value if the first concentration value is within a first threshold range;
if the first concentration value is beyond the first threshold range but within the second threshold range, the controller dilutes or concentrates the sample, and then the detector measures the sample again and obtains a second concentration value according to the measured value and the dilution or concentration ratio; determining, by the processor, a final concentration value from the second concentration value if the second concentration value is within a third threshold range; if the second concentration value exceeds a third threshold range, the processor determines a final concentration value according to the first concentration value;
if the first concentration value exceeds a first threshold range and exceeds a second threshold range, the controller dilutes or concentrates the sample, then the detector measures the sample again and obtains a second concentration value according to the measured value and the dilution or concentration ratio, and the processor determines a final concentration value according to the second concentration value.
12. The sample analyzer of claim 11, wherein the first threshold range is less than D1, the second threshold range is (D1, D1+ a1), the third threshold range is (D1-B1, D1+ B1);
the controller dilutes the sample if the first concentration value is outside a first threshold range.
13. The sample analyzer of claim 11, wherein the first threshold range is greater than D2, the second threshold range is (D2-a2, D2), the third threshold range is (D2-B2, D2+ B2); the controller concentrates the sample if the first concentration value is outside a first threshold range.
14. The sample analyzer of claim 11, wherein the first threshold range is determined by the processor from historical measurement data.
15. The sample analyzer of claim 11, wherein the second threshold range and/or the third threshold range is related to the first threshold range.
16. The sample analyzer of claim 11, wherein the detector is configured to:
measuring a sample to obtain an intermediate parameter;
determining the substance concentration according to the intermediate parameter and a preset corresponding relation; the preset correspondence is a correspondence between the intermediate parameter and the concentration.
17. The sample analyzer of claim 16 wherein the intermediate parameter comprises light flux data or clotting time.
18. The sample analyzer of claim 11, wherein the sample analyzer comprises a coagulation analyzer or a biochemical immunoassay analyzer.
19. A computer storage medium, characterized in that a program is stored in the computer storage medium, which program, when being executed by a processor, carries out the method of any one of claims 1 to 10.
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| CN115145431A (en) * | 2022-07-06 | 2022-10-04 | 中元汇吉生物技术股份有限公司 | Sample processing method, system, device, readable medium and sample analyzer |
| WO2022205600A1 (en) * | 2021-03-29 | 2022-10-06 | 深圳市科曼医疗设备有限公司 | High-concentration sample measurement and time sequence calling method |
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