CN114609019B - Detection method of sample analyzer and sample analyzer - Google Patents
Detection method of sample analyzer and sample analyzer Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 204
- 239000007788 liquid Substances 0.000 claims abstract description 140
- 239000002699 waste material Substances 0.000 claims abstract description 140
- 238000013507 mapping Methods 0.000 claims abstract description 45
- 238000000034 method Methods 0.000 claims description 20
- 238000012360 testing method Methods 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 13
- 239000010437 gem Substances 0.000 claims description 9
- 229910001751 gemstone Inorganic materials 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 8
- 238000012937 correction Methods 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 51
- 238000010586 diagram Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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Abstract
The application provides a detection method of a sample analyzer and the sample analyzer. The detection method is applied to a sample analyzer, and particularly, the sample analyzer comprises a detection pool and a waste liquid pool communicated with the detection pool, wherein the waste liquid pool is used for collecting a detected sample which is discharged from the detection pool and is detected when the detected sample is detected, and the detection method comprises the following steps: acquiring a mapping relation, wherein the mapping relation comprises a corresponding relation between a pressure value in the waste liquid pool and a flow value in the detection pool; when a sample to be detected is detected, acquiring a pressure value in a waste liquid pool; obtaining a flow value in the detection tank corresponding to the pressure value based on the pressure value in the waste liquid tank and the mapping relation; and detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result. The detection method of the sample analyzer is simple, the hardware cost of the sample analyzer can be saved, and the accuracy of sample detection can be improved.
Description
Technical Field
The present application relates to the field of medical devices, and in particular, to a method for detecting a sample analyzer and a sample analyzer.
Background
The blood cell analyzer is a device capable of counting and classifying cells in blood, and is used for detecting white blood cells by using methods such as impedance, flow fluorescence and the like, and detecting red blood cells and PLT by using common impedance and sheath flow impedance.
In the prior art, when a sample analyzer detects common impedance of a sample, an algorithm calculates a test result through theoretical sample statistics. However, the sample statistic is influenced by factors such as the size and stability of a pressure source in the detection process of the instrument, so that the real sample statistic is different from the theoretical sample statistic, that is, the theoretical sample statistic cannot truly reflect the real sample statistic in the test process, and therefore a certain error exists in the test result.
Disclosure of Invention
The application provides a detection method of a sample analyzer and the sample analyzer, which are used for solving the technical problem that in the prior art, theoretical sample statistics cannot truly reflect real sample statistics in a secondary test process, so that the accuracy of a test result is not high.
In order to solve the technical problem, the application adopts a technical scheme that: the detection method of the sample analyzer is applied to the sample analyzer, the sample analyzer comprises a detection pool and a waste liquid pool communicated with the detection pool, the waste liquid pool is used for collecting a detected sample which is discharged from the detection pool and is detected when the detected sample is detected, and the detection method comprises the following steps: acquiring a mapping relation, wherein the mapping relation comprises a corresponding relation between a pressure value in the waste liquid pool and a flow value in the detection pool; when a sample to be detected is detected, acquiring a pressure value in a waste liquid pool; obtaining a flow value in the detection tank corresponding to the pressure value in the waste liquid tank based on the pressure value in the waste liquid tank and the mapping relation; and detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result.
Further, the sample analyzer still includes pressure building device and pressure sensor who is connected with the waste liquid pond, when detecting the sample that awaits measuring, the step of obtaining the pressure value in the waste liquid pond includes: when a sample to be detected is detected, the pressure in the waste liquid pool is adjusted to be within a target pressure range through a pressure building device; and acquiring a pressure value in the waste liquid pool through a pressure sensor.
Further, acquiring the pressure value in the waste liquid pool by the pressure sensor comprises: acquiring a first pressure value at each moment in the waste liquid tank through a pressure sensor; and calculating the average value of all the first pressure values, and taking the average value as the pressure value in the waste liquid pool.
Further, the detection pool comprises a counting pool, and the detection method further comprises the following steps: when a sample to be detected is detected, the particle number of the sample to be detected is obtained through a counting cell; the method comprises the following steps of carrying out detection analysis on a sample to be detected based on a flow value in a detection pool, and obtaining a detection result, wherein the steps comprise: and obtaining a concentration value of the cells of the sample to be detected based on the flow value in the detection pool and the particle number of the sample to be detected.
Further, the step of obtaining the concentration value of the cells of the sample to be detected based on the flow value in the detection tank and the number of the particles of the sample to be detected comprises: acquiring a correction coefficient; and calculating the concentration value of the cells of the sample to be detected based on the flow value in the detection pool, the particle number of the sample to be detected and the correction coefficient.
Further, a flow detection device is arranged between the waste liquid tank and the detection tank, the flow detection device is used for detecting a flow value in the detection tank, and before the step of obtaining the mapping relation, the detection method further comprises the following steps: changing the pressure in the waste liquid pools by a pressure building device, and acquiring pressure values in the waste liquid pools by pressure sensors; and respectively acquiring flow values in the detection tanks corresponding to the pressure values in the waste liquid tanks through the flow detection device to obtain a mapping relation.
Further, the step of obtaining the mapping relationship includes: and fitting a curve of the flow values in the detection tanks along with the change of the pressure values in the waste liquid tanks based on the pressure values in the waste liquid tanks and the flow values in the detection tanks corresponding to the pressure values in the waste liquid tanks to obtain a mapping relation.
Further, the sample analyzer further comprises a sample adding unit, and when the sample to be detected is detected, before the step of obtaining the pressure value in the waste liquid pool, the detection method further comprises the following steps: and adding a sample to be detected and a reagent into the detection pool through the sample adding unit.
Further, the sample analyzer further comprises a cleaning unit, the cleaning unit is connected with the detection pool, the sample to be detected is detected and analyzed based on the flow value in the detection pool, and after the step of obtaining the detection result, the detection method further comprises the following steps: and washing the detection pool by the washing unit.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a sample analyzer comprising: the device comprises a detection pool and a waste liquid pool, wherein the detection pool is communicated with the waste liquid pool, and the waste liquid pool is used for collecting a sample to be detected in the detection pool when the sample to be detected is detected; a control unit for implementing the detection method of the sample analyzer of any of the above embodiments.
The beneficial effect of this application is: in contrast to the prior art, the detection method of the sample analyzer of the present application includes: acquiring a mapping relation, wherein the mapping relation comprises a corresponding relation between a pressure value in the waste liquid pool and a flow value in the detection pool; when a sample to be detected is detected, acquiring a pressure value in a waste liquid pool; obtaining a flow value in the detection tank corresponding to the pressure value in the waste liquid tank based on the pressure value in the waste liquid tank and the mapping relation; and detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result. According to the method and the device, the flow value in the detection pool corresponding to the sample detection is obtained through the mapping relation between the pressure and the flow, and the flow value can accurately reflect the real flow in the secondary test process, so that the accuracy of the detection result of the sample analyzer can be improved, the detection method has low requirements on the hardware of the sample analyzer, and the production cost of the sample analyzer can be reduced.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
FIG. 1 is a schematic block diagram of one embodiment of a sample analyzer provided herein;
FIG. 2 is a schematic flow chart diagram of one embodiment of a detection method for a sample analyzer provided herein;
FIG. 3 is a flowchart illustrating an embodiment of step S22 in FIG. 2;
FIG. 4 is a flowchart illustrating an embodiment of step S222 in FIG. 3;
FIG. 5 is a schematic flow chart diagram of another embodiment of a detection method of a sample analyzer provided herein;
fig. 6 is a schematic structural diagram of another embodiment of a sample analyzer provided herein.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that if directional indications (such as up, down, left, right, front, back, 8230; \8230;) are referred to in the embodiments of the present application, the directional indications are only used to explain the relative positional relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indications are correspondingly changed.
In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present application, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present application.
The application provides a detection method of a sample analyzer and the sample analyzer.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a sample analyzer 10 provided in the present application, for analyzing and detecting a sample. Specifically, the sample analyzer 10 includes: a detection cell 11 and a waste liquid cell 12. The detection cell 11 is connected with the waste liquid cell 12 and is communicated with the waste liquid cell 12. When the sample analyzer 10 detects a sample, the sample to be detected in the detection cell 11 enters the waste liquid cell 12, that is, the waste liquid cell 12 is used for collecting the detected sample discharged from the detection cell 11, so as to perform sample detection in a matching manner.
Specifically, as shown in fig. 2, fig. 2 is a schematic flow chart of an embodiment of a detection method of a sample analyzer provided in the present application, where the detection method includes:
s21: and acquiring a mapping relation, wherein the mapping relation comprises a corresponding relation between a pressure value in the waste liquid pool and a flow value in the detection pool.
When a sample to be measured is measured, a predetermined mapping relation is obtained. The mapping relationship comprises: the pressure value in the waste liquid tank 12 and the flow value in the detection tank 11. For example, when the pressure in the waste liquid tank 12 is a pascal, the flow value in the detection tank 11 is a ', and when the pressure in the waste liquid tank 12 is B pascal, the flow value in the detection tank 11 is B'. That is, each pressure value in the waste liquid tank 12 can find the corresponding flow value in the detection tank 11 in the mapping relation. Wherein, the flow value in the detection cell 11 represents the flow of the sample to be detected which is discharged from the detection cell 11 to the waste liquid cell 12.
For example, the detection tank 11 may include a counting tank (not shown), the counting tank is provided with a jewel hole, and the sample to be measured enters the waste liquid tank 12 after passing through the jewel hole, so as to perform particle counting on the sample to be measured. The flow in the detection pool 11 refers to the flow of a sample to be detected passing through the jewel hole, and the pressure value of the waste liquid pool 12 refers to the pressure value in the waste liquid pool 12 when the sample to be detected enters the waste liquid pool 12 from the jewel hole.
Alternatively, the mapping relationship may be a graph, i.e., a graph of the flow value in the detection tank 11 with the pressure value in the waste liquid tank 12. In another embodiment, the mapping relationship may be a table, and the table includes the pressure value in each waste liquid tank 12 and the flow value in the detection tank 11 corresponding to the pressure value.
S22: when detecting the sample to be detected, the pressure value in the waste liquid pool is obtained.
When a sample to be detected is actually detected, the sample to be detected enters the waste liquid pool 12 from the detection pool 11, and in the process, a pressure value in the waste liquid pool 12 is obtained.
Alternatively, the sample analyzer 10 may automatically measure the pressure value within the waste reservoir 12 to increase the degree of automation of the sample analyzer 10. In other embodiments, the sample analyzer 10 may also obtain the pressure value within the waste reservoir 12 from an external measurement device.
In a specific embodiment, as shown in fig. 1, the sample analyzer 10 may further include a pressure building device 13 and a pressure sensor 14 connected to the waste liquid pool 12, where the pressure building device 13 is configured to adjust the pressure in the waste liquid pool 12, and the pressure sensor 14 is configured to measure the pressure value in the waste liquid pool 12, and as shown in fig. 3, the step of obtaining the pressure value in the waste liquid pool 12 when detecting the sample to be detected includes:
s221: when a sample to be detected is detected, the pressure in the waste liquid pool is adjusted to be within a target pressure range through the pressure building device.
When a sample to be detected is detected, the pressure in the waste liquid tank 12 is adjusted by the pressure building device 13, so that the pressure in the waste liquid tank 12 reaches a target pressure range.
S222: and acquiring a pressure value in the waste liquid pool through a pressure sensor.
When the sample to be measured enters the waste liquid tank 12 from the detection tank 11, the pressure sensor 14 measures the pressure value in the waste liquid tank 12.
In a specific embodiment, the pressure sensor 14 may acquire a pressure value at a certain time in the waste liquid tank 12 during the sample detection process, and use the pressure value at the certain time as the pressure value in the waste liquid tank 12.
In another embodiment, since the pressure value in the waste liquid tank 12 may fluctuate within a range, the present application may use the average pressure value in the waste liquid tank 12 as the pressure value in the waste liquid tank 12 to improve the accuracy of the detection result. Specifically, as shown in fig. 4, the step of acquiring the pressure value in the waste liquid tank 12 through the pressure sensor 14 may include:
s2221: and acquiring a first pressure value at each moment in the waste liquid pool through a pressure sensor.
When a sample to be detected is detected, the sample to be detected enters the waste liquid pool 12 from the detection pool 11, and a first pressure value in the waste liquid pool 12 at each moment is obtained through the pressure sensor 14. Since the pressure in the waste liquid tank 12 may fluctuate within a range, the first pressure values obtained by the pressure sensor 14 at different times in the waste liquid tank 12 may be different.
S2222: and calculating the average value of all the first pressure values, and taking the average value as the pressure value in the waste liquid pool.
After obtaining the first pressure value at each time in the waste liquid tank 12, the sample analyzer 10 calculates an average value of all the first pressure values, and uses the average value as the pressure value in the waste liquid tank 12. By the method, the inaccuracy of the measurement result caused by the pressure fluctuation in the waste liquid pool 12 can be avoided, and the accuracy of the sample detection result is improved.
S23: and obtaining a flow value in the detection pool corresponding to the pressure value based on the pressure value in the waste liquid pool and the mapping relation.
After the pressure value and the mapping relation of the waste liquid pool 12 are obtained, the flow value in the detection pool 11 corresponding to the pressure value is found out from the mapping relation according to the pressure value in the waste liquid pool 12. Compared with the prior art that a fixed flow value is used, the method provided by the application enables the acquired flow value in the detection pool 11 to be closer to the real flow in the current test process, so that the accuracy of sample detection can be improved.
And when the sample analyzer 10 is used, the flow value in the detection cell 11 can be obtained without using a flow detection device, so that the production cost of the sample analyzer 10 is reduced.
S24: and detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result.
After the flow value of the detection cell 11 is obtained, the sample analyzer 10 performs detection analysis on the sample based on the flow value to obtain a detection result.
In one particular embodiment, the sample analyzer 10 can be used to perform cell counting and classification tests on a sample to be tested. The sample analyzer 10 may count the cells by impedance detection. Specifically, the detection cell 11 may include a counting cell, and when a sample to be detected is detected, the number of particles of the sample to be detected is obtained through the counting cell. Can be equipped with the precious stone hole in the counting cell, the sample that awaits measuring passes through the precious stone hole to obtain the particle number of the sample that awaits measuring. And then obtaining the concentration value of the cells of the sample to be detected based on the flow value in the detection cell 11 and the particle number of the sample to be detected.
Further, in order to improve the accuracy of the sample detection result, the sample analyzer 10 may further obtain a correction coefficient, and calculate the concentration value of the cells of the sample to be detected based on the flow value in the detection cell 11, the number of particles of the sample to be detected, and the correction coefficient.
It is understood that after the flow value of the detection cell 11 is obtained, other items of detection may be performed on the sample to be detected based on the flow value, which is not listed here.
Different from the prior art, the detection method of the sample analyzer has the advantages that the process is simple, the hardware requirement on the sample analyzer 10 is low, and the production cost of the sample analyzer 10 can be reduced. Moreover, the flow value in the detection cell in the prior art is generally a fixed value, and according to the application, the corresponding flow value in the detection cell 11 is obtained according to the pressure value in the waste liquid cell 12 and the mapping relation, so that the obtained flow value in the detection cell 11 is more accurate, and the accuracy of the sample detection result can be improved.
In this embodiment, as shown in fig. 6, a flow detection device 17 is disposed between the waste liquid tank 12 and the detection tank 11, and the flow detection device 17 is configured to detect a flow value in the detection tank 11, so as to determine a corresponding relationship between the flow in the detection tank 11 and a pressure value in the waste liquid tank 12 through the flow detection device 17. When the mapping is determined, the sample analyzer 10 may obtain the flow rate value in the detection cell 11 through the mapping without installing the flow rate detection device 17 during the actual use. For example, the flow rate detection device 17 may be installed before the sample analyzer 10 is shipped from the factory to obtain the mapping relationship, and the flow rate detection device 17 may not be installed when the sample analyzer 10 is shipped from the factory to reduce the production cost of the sample analyzer 10.
In this embodiment, the detection method includes the following steps:
s51: the pressure in the waste liquid pool is changed through the pressure building device.
A test fluid, which may be water, may be added to the test cell 11 in the sample analyzer 10 to simulate the sample to be tested. During measurement, the test liquid enters the waste liquid tank 12 from the detection tank 11, so that the mapping relation between the pressure value in the waste liquid tank 12 and the flow value in the detection tank 11 is measured through the process. Further, during testing, the pressure in the waste liquid tank 12 is changed by the pressure buildup device 13 so as to achieve different target pressures in the waste liquid tank 12.
S52: pressure values in a plurality of waste liquid pools are obtained through the pressure sensors.
When the waste liquid tank 12 is in different pressure states, a plurality of different pressure values in the waste liquid tank 12 are obtained sequentially through the pressure sensor 14.
S53: and respectively acquiring flow values in the detection tanks corresponding to the pressure values in the waste liquid tanks through the flow detection device to obtain a mapping relation.
During testing, the flow value in the detection cell 11 corresponding to each pressure value in the waste liquid cell 12 is obtained through the flow detection device 17, and in this way, the corresponding relation between each pressure value in the waste liquid cell 12 and the flow in the detection cell 11 can be obtained. The method for acquiring the mapping relation is simple and high in accuracy.
In another embodiment, the pressure building device 13 may change the target pressure value in the waste liquid tank 12 during counting, count the flow of the flow detection device under the state of multiple pressure values, and based on the flow values in the detection tank 11 corresponding to the pressure values in the waste liquid tank 12, then fit a curve of the flow values in the detection tank 11 changing with the pressure values in the waste liquid tank 12 to obtain the mapping relationship between the flow values in the detection tank 11 and the pressure values in the waste liquid tank 12. In this way, the flow rate in the detection cell 11 corresponding to each pressure value in the waste liquid cell 12 does not need to be measured, and the detection efficiency is improved.
S54: and acquiring a mapping relation, wherein the mapping relation comprises a corresponding relation between a pressure value in the waste liquid pool and a flow value in the detection pool.
Step S54 is the same as step S21, and will not be described herein.
S55: and adding a sample to be detected and a reagent into the detection pool through the sample adding unit.
As shown in fig. 6, the sample analyzer 10 may further include a sample adding unit 15, when detecting a sample to be detected, the sample to be detected and a reagent are first added into the detection cell 11 through the sample adding unit 15, and after the sample to be detected and the reagent are fully reacted, the sample to be detected enters the waste liquid cell 12 from the detection cell 11, so as to detect the sample to be detected.
S56: when detecting the sample to be detected, the pressure value in the waste liquid pool is obtained.
Step S56 is the same as step S22, and is not described herein again.
S57: and obtaining a flow value in the detection tank corresponding to the pressure value based on the pressure value in the waste liquid tank and the mapping relation.
Step S57 is the same as step S23, and is not described herein again.
S58: and detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result.
Step S58 is the same as step S24, and is not described herein again.
S59: and washing the detection pool by the washing unit.
As shown in fig. 6, the sample analyzer 10 may further include a washing unit 16, and the washing unit 16 is used to wash the detection cell 11. That is, after the sample to be measured is detected in the detection cell 11, the sample analyzer 10 cleans the detection cell 11 by the cleaning unit 16.
Specifically, as shown in fig. 6, the cleaning unit 16 may include a reservoir 161 and a waste liquid collecting device 162, after the detection cell 11 has been detected, under the action of a pressure source (not shown), liquid in the reservoir 161 is made to enter the detection cell 11 to wash the detection cell 11, and the washed waste liquid is collected by the waste liquid collecting device 162.
In this embodiment, the mapping relationship between the pressure value in the waste liquid tank 12 and the flow value in the detection tank 11 is obtained by the flow detection device 17 and the pressure sensor 14, the obtaining method of the mapping relationship is simple, the reliability is high, when a sample to be detected is detected, the accuracy of the flow value of the detection tank obtained based on the mapping relationship is high, and the reliability of sample detection can be improved.
The application also provides a sample analyzer, which comprises a detection pool, a waste liquid pool and a control unit, wherein the waste liquid pool is used for collecting the sample to be detected in the detection pool when the sample to be detected is detected, and the control unit can realize the detection method of the sample analyzer of any embodiment.
For the structure of the sample analyzer, please refer to fig. 1 and fig. 6 and the related text description of the above embodiments, which are not repeated herein. For a method for detecting a sample analyzer, please refer to the description of any of the above embodiments, which is not repeated herein.
The above description is only an embodiment of the present application, and is not intended to limit the scope of the present application, and all equivalent structures or equivalent processes performed by the present application and the contents of the attached drawings, which are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (8)
1. A detection method of a sample analyzer is characterized in that the detection method is applied to a sample analyzer, the sample analyzer counts samples to be detected through an impedance detection method, the sample analyzer comprises a detection cell and a waste liquid cell communicated with the detection cell, the detection cell comprises a counting cell, a gem hole is arranged in the counting cell, the waste liquid cell is used for collecting the samples to be detected which are discharged from the detection cell and are detected, and the detection method comprises the following steps:
acquiring a mapping relation, wherein the mapping relation comprises a corresponding relation between a pressure value in the waste liquid pool and a flow value in the detection pool;
when the sample to be detected is detected, acquiring a pressure value in the waste liquid pool when the sample to be detected enters the waste liquid pool from the detection pool, wherein the pressure value in the waste liquid pool is the pressure value in the waste liquid pool when the sample to be detected enters the waste liquid pool from the jewel hole;
obtaining a flow value in the detection pool corresponding to the pressure value in the waste liquid pool based on the pressure value in the waste liquid pool and the mapping relation so as to obtain the flow value of the detection pool without a flow detection device when the sample analyzer is used, wherein the flow value represents the flow of a sample to be detected which is discharged from the detection pool to the waste liquid pool, and the flow value is the flow of the sample to be detected which passes through the jewel hole;
detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result,
the sample analyzer further comprises a pressure building device and a pressure sensor which are connected with the waste liquid pool, and when the sample to be detected is detected, the step of obtaining the pressure value in the waste liquid pool comprises the following steps: when the sample to be detected is detected, the pressure in the waste liquid pool is adjusted to be within a target pressure range through the pressure building device; the pressure sensor is used for acquiring the pressure value in the waste liquid pool,
a flow detection device is arranged between the waste liquid pool and the detection pool, the flow detection device is used for detecting the flow value in the detection pool, and before the step of obtaining the mapping relation, the detection method further comprises the following steps: changing the pressure in the waste liquid pool through the pressure building device, and acquiring pressure values in the waste liquid pools through the pressure sensors; and respectively acquiring flow values in the detection tanks corresponding to the pressure values in the waste liquid tanks through the flow detection device so as to obtain the mapping relation.
2. The detection method according to claim 1, wherein the obtaining of the pressure value in the waste liquid pool by the pressure sensor comprises:
acquiring a first pressure value at each moment in the waste liquid pool through the pressure sensor;
and calculating the average value of all the first pressure values, and taking the average value as the pressure value in the waste liquid pool.
3. The detection method according to claim 1, characterized in that the detection method further comprises:
when the sample to be detected is detected, the particle number of the sample to be detected is obtained through the counting cell;
the step of detecting and analyzing the sample to be detected based on the flow value in the detection pool to obtain a detection result comprises the following steps: and obtaining a concentration value of the cells of the sample to be detected based on the flow value in the detection pool and the particle number of the sample to be detected.
4. The method according to claim 3, wherein the step of obtaining the concentration value of the cells of the sample to be tested based on the flow value in the test cell and the number of the particles of the sample to be tested comprises:
acquiring a correction coefficient;
and calculating the concentration value of the cells of the sample to be detected based on the flow value in the detection pool, the number of the particles of the sample to be detected and the correction coefficient.
5. The detection method according to claim 1, wherein the step of obtaining the mapping relationship comprises:
and fitting a curve of the flow value in the detection pool along with the change of the pressure value in the waste liquid pool based on the plurality of pressure values in the waste liquid pool and the flow value in the detection pool corresponding to the pressure values so as to obtain the mapping relation.
6. The detection method according to claim 1, wherein the sample analyzer further comprises a sample adding unit, and before the step of obtaining the pressure value in the waste liquid pool when the sample to be detected is detected, the detection method further comprises:
and adding a sample to be detected and a reagent into the detection pool through the sample adding unit.
7. The detection method according to claim 1, wherein the sample analyzer further comprises a washing unit, the washing unit is connected to the detection cell, and after the step of performing detection analysis on the sample to be detected based on the flow value in the detection cell to obtain the detection result, the detection method further comprises:
and flushing the detection cell through the cleaning unit.
8. A sample analyzer, comprising:
the device comprises a detection pool and a waste liquid pool, wherein the detection pool is communicated with the waste liquid pool, and the waste liquid pool is used for collecting a sample to be detected in the detection pool when the sample to be detected is detected;
a control unit for implementing the detection method of the sample analyzer of any one of claims 1-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210520889.0A CN114609019B (en) | 2022-05-13 | 2022-05-13 | Detection method of sample analyzer and sample analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210520889.0A CN114609019B (en) | 2022-05-13 | 2022-05-13 | Detection method of sample analyzer and sample analyzer |
Publications (2)
| Publication Number | Publication Date |
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