CN111855977B - Blood analysis system, cleansing liquid calculation device, and cleansing liquid calculation method - Google Patents
Blood analysis system, cleansing liquid calculation device, and cleansing liquid calculation method Download PDFInfo
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Abstract
The invention discloses a blood analysis system, a cleaning solution calculating device and a cleaning solution calculating method, wherein a blood analyzer comprises a constant current source and a counting cell unit, wherein the constant current source and the counting cell unit form a first loop for performing blood analysis on a sample to be detected in the counting cell unit; the cleaning liquid calculating device includes: the counting cell monitoring unit and the constant current source form a second loop to monitor the resistance change value of the sample to be tested before and after blood analysis in the counting cell unit; and the cleaning liquid calculating device correspondingly calculates and obtains the consumption of the cleaning liquid according to the monitored resistance change value. Through the mode, the cleaning effect can be ensured and the waste of the cleaning liquid is avoided.
Description
Technical Field
The present invention relates to the field of a cleaning solution calculation technology, and in particular, to a blood analysis system, a cleaning solution calculation device, and a cleaning solution calculation method.
Background
A blood analyzer is a commonly used sample analyzer, and a part of blood adheres to an analysis cell when a blood sample to be measured passes through a blood counting cell unit for analysis. In order to ensure the reliability of the detection result, the analysis cell after the sample to be detected is detected needs to be cleaned by using a cleaning solution.
In the prior art, the adding amount of the cleaning fluid is basically a fixed value and cannot be determined according to the amount of blood adhered to the analysis pool, so that the problem of inaccurate using amount of the cleaning fluid is caused. That is, when the amount of the cleaning solution is insufficient, the analysis cell is not thoroughly cleaned; when the amount of the cleaning solution is too much, the cleaning solution is wasted. Therefore, the cleaning effect cannot be guaranteed, and the waste of cleaning liquid can be caused.
In a long-term research and development process, the inventor of the application finds that the consumption of the cleaning solution in the existing blood analyzer can not ensure the cleaning effect and avoid the waste of the cleaning solution.
Disclosure of Invention
The invention mainly solves the technical problem of providing a blood analyzer, a cleaning solution calculating device and a cleaning solution calculating method, which can ensure the cleaning effect and avoid the waste of the cleaning solution.
In order to solve the technical problems, the invention adopts a technical scheme that: a cleaning solution calculating device for a blood analyzer is provided.
The blood analyzer comprises a constant current source and a counting cell unit, wherein the constant current source and the counting cell unit form a first loop for performing blood analysis on a sample to be detected in the counting cell unit; the cleaning liquid calculating device includes: the counting cell monitoring unit and the constant current source form a second loop to monitor the resistance change value of the sample to be tested before and after blood analysis in the counting cell unit; and the cleaning liquid calculating device correspondingly calculates and obtains the consumption of the cleaning liquid according to the monitored resistance change value.
In order to solve the technical problem, the invention adopts another technical scheme that: a blood analysis system is provided.
The blood analysis system comprises a blood analyzer and any one of the cleaning solution calculating devices.
In order to solve the technical problem, the invention adopts another technical scheme that: a method of calculating a cleaning solution for a blood analyzer is provided.
Wherein, the method comprises the following steps:
providing any one of the cleaning solution calculating devices;
monitoring resistance change values of the counting cell unit before and after blood analysis of a sample to be detected;
and correspondingly calculating to obtain the use amount of the cleaning liquid according to the monitored resistance change value.
The invention has the beneficial effects that: different from the prior art, the invention monitors the resistance change value of the counting cell unit before and after the blood analysis of the sample to be detected through the second loop formed by the constant current source in the blood analyzer and the counting cell monitoring unit, and correspondingly calculates and obtains the dosage of the cleaning solution according to the resistance change value monitored by the cleaning solution calculating device. The amount of the cleaning liquid is determined according to the change of the resistance of the counting cell before and after the sample to be detected is detected, namely the amount of the cleaning liquid is related to the amount of blood adhered to the counting cell unit, so that the cleaning effect can be guaranteed by the amount of the cleaning liquid, and the waste of the cleaning liquid is avoided.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic diagram of the structure of one embodiment of a blood analyzer;
FIG. 2 is a schematic structural view of a first embodiment of a cleaning solution calculating apparatus for a blood analyzer according to the present invention;
FIG. 3 is a schematic circuit diagram of a first embodiment of a wash solution calculating device for a blood analyzer according to the present invention;
FIG. 4 is a schematic structural view of a second embodiment of a cleaning solution calculating apparatus for a blood analyzer according to the present invention;
FIG. 5 is a schematic structural view of a third embodiment of a cleaning solution calculating apparatus for a blood analyzer according to the present invention;
FIG. 6 is a schematic structural view of a fourth embodiment of a cleaning solution calculating apparatus for a blood analyzer according to the present invention;
FIG. 7 is a schematic block diagram of one embodiment of a blood analyzer system of the present invention;
FIG. 8 is a schematic flow chart diagram illustrating one embodiment of a cleaning solution calculation method for a blood analyzer according to the present invention;
fig. 9 is a flowchart illustrating an embodiment of step S820 in fig. 8.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting of the application. It should be further noted that, for the convenience of description, only some of the structures related to the present application are shown in the drawings, not all of the structures. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The terms "first", "second", etc. in this application are used to distinguish between different objects and not to describe a particular order. Furthermore, the terms "include" and "have," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
Before describing the technical scheme of the present invention, a process of blood analysis for detecting a sample to be detected is described, please refer to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a blood analyzer 100, the blood analyzer 100 includes a first loop formed by a constant current source 110 and a counting cell unit (not identified), the counting cell unit specifically includes a counting cell (not identified), a diluent 140 contained in the counting cell, a front cell electrode 131 and a rear cell electrode 132 immersed in the diluent, wherein the front cell electrode 131 and the rear cell electrode 132 are respectively connected with the constant current source 110, and the sample to be detected is contained in the counting cell; further, the counting chamber includes a front chamber 121 and a rear chamber 122, and the front chamber 121 and the rear chamber 122 are communicated through the counting hole 150. When the blood analysis is performed on the sample to be detected, the sample to be detected reaches the rear cell 122 through the counting hole 150, and at the moment when the sample (blood cells) to be detected passes through the counting hole 150, the impedance in the first loop changes, the amplitude and the width of the voltage pulse detected by the detection mechanism at the two ends of the front cell electrode 131 and the rear cell electrode 132 can be used for determining the size of the blood cells passing through the detection hole, and the blood cells can be counted according to the number of the voltage pulse.
And the in-process of testing the sample to be tested, the sample to be tested can remain in the counting cell, therefore, before and after testing the sample to be tested, the resistance value of the counting cell unit can change. According to the experimental results of the inventor of the present invention, it is shown that the resistance value of the cell unit and the amount of the sample to be measured remaining in the counting cell satisfy a certain relationship, the amount of the sample to be measured remaining in the counting cell can be judged according to the change value of the resistance before and after the detection of the sample to be measured, and the appropriate amount of the cleaning solution can be determined according to the amount of the sample to be measured remaining in the counting cell.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a first embodiment of a cleaning solution calculating apparatus for a blood analyzer according to the present invention, wherein the blood analyzer includes a constant current source 210 and a cell unit 220, and the constant current source 210 and the cell unit 220 form a first circuit 230 for performing blood analysis on a sample to be measured in the cell unit 220. The cleaning liquid calculation device 200 includes: a counting cell monitoring unit 240, wherein the counting cell monitoring unit 240 and the constant current source 210 form a second loop 250 to monitor the resistance change value before and after the blood analysis of the sample to be detected in the counting cell unit 220; the cleaning solution calculating device 200 correspondingly calculates and obtains the usage amount of the cleaning solution according to the monitored resistance change value.
In the present embodiment, the second circuit 250 formed by the constant current source 210 and the cuvette monitor unit 240 in the blood analyzer monitors the resistance change value before and after the blood analysis of the sample to be measured by the cuvette unit 220, and the amount of the cleaning solution to be used is calculated and acquired in accordance with the resistance change value monitored by the cleaning solution calculation device 200. Because the amount of the cleaning solution is determined according to the change of the resistance of the counting cell before and after the sample to be detected is detected, namely the amount of the cleaning solution is related to the amount of the blood adhered to the counting cell unit 220, the amount of the cleaning solution can ensure the cleaning effect and avoid the waste of the cleaning solution.
Further, referring to fig. 2 and 3 together, fig. 3 is a first cleaning solution calculating device for a blood analyzer according to the present inventionIn the circuit schematic diagram of the embodiment, it can be seen that the branch in which the counting cell unit 220 is located is connected in parallel with the branch in which the counting cell monitoring unit 240 is located. Wherein R is1Is the equivalent resistance of the cell unit 220, I1Is the current in the cell unit 220; r2Is the equivalent resistance of the cell monitoring unit 240, I2In order to monitor the current in the cell monitoring unit 240, I ═ I can be known from the current-voltage relationship of the parallel circuit1+I2，V1＝V2Wherein V is1＝I1*R1，V2＝I2*R2。
Further, the constant current source 210 provides a current value I that is known, and to ensure that the cell unit 220 needs to operate at a constant current, the current I through the branch of the cell monitoring unit 240 is made to pass2Is much smaller than the current I of the branch in which the counting cell unit 220 is positioned1. That is, although the cell monitoring unit 240 divides a part of the current provided by the constant current source 210, the current I of the branch in which the cell unit 220 is located1Is hardly affected, i.e. I1I, therefore, the accuracy of the counting result of the counting cell unit 220 can be ensured. Specifically, taking the sample a to be tested as an example, immediately before performing blood analysis on the sample a to be tested, the current I of the branch where the primary counting cell monitoring unit 240 is located is detectedA1Then, at this time, the current I of the branch in which the cell unit 220 is located11＝I-IA1(ii) a After the detection of the sample A to be detected is finished, the current I of the branch where the counting cell monitoring unit 240 is located is detected againA2Then, at this time, the current I of the branch in which the cell unit 220 is located12＝I-IA2。
In order to determine the resistance change of the cuvette unit 220 before and after the blood analysis of the sample to be tested, it is also necessary to determine the voltage across the cuvette unit 220. Since the counting cell monitoring unit 240 is connected in parallel with the counting cell unit 220, it can be considered that the voltage of the branch in which the counting cell monitoring unit 240 is located is the same as the voltage of the branch in which the counting cell unit 220 is located, and the voltage at the two ends of the counting cell monitoring unit 240 before and after the blood analysis of the sample to be detected by monitoring the counting cell unit 220 can determine the voltage of the sample to be detectedThe voltage value across the cell unit 220 is counted before and after the sample to be tested is subjected to blood analysis. Specifically, just before blood analysis is performed on the sample a to be detected, the voltage V of the branch in which the primary counting cell monitoring unit 240 is located is detectedA1(ii) a After the detection of the sample A to be detected is finished, the voltage V at the two ends of the counting cell monitoring unit 240 is detected againA2。
Specifically, the voltage across the cell monitoring unit 440 is determined by the current in the cell monitoring unit 440. In one embodiment, please refer to fig. 4, fig. 4 is a schematic structural diagram of a second embodiment of a cleaning solution calculating device for a blood analyzer according to the present invention, different from the cleaning solution calculating device for a blood analyzer in fig. 2, a counting chamber monitoring unit 440 in the present embodiment specifically includes a current amplifying module 441 and a signal converting module 442, wherein the current amplifying module 441 is used for amplifying a current in the counting chamber monitoring unit 440; the signal conversion module 442 is connected to the current amplification module, and is configured to convert the amplified current signal into a voltage signal.
In this embodiment, the current I passing through the branch of the cell monitoring unit 4402Is much smaller than the current I of the branch in which the counting cell unit 220 is positioned1In order to obtain the voltage across the cell control unit 440, the current I passing through the branch of the cell monitoring unit 440 is amplified by the current amplifying module 4412The amplification is carried out, and the amplification factor can be determined according to the actual requirement. In one embodiment, the current amplifying module 441 includes a transistor, wherein a base of the transistor is connected to the constant current source 210, and a collector of the transistor is connected to the signal converting module 442, that is, the amplified current flows through the signal converting module 442, and the current signal is converted into a voltage signal, that is, a voltage across the cell monitoring unit 440 is obtained.
Further, referring to fig. 5, fig. 5 is a schematic structural diagram of a third embodiment of a cleansing liquid calculating device for a blood analyzer according to the present invention, and unlike the cleansing liquid calculating device for a blood analyzer in fig. 4, the counting cell monitoring unit 540 in the present embodiment specifically includes a current amplifying module 541, a signal converting module 542, an analog-to-digital converting module 543, and a data processing module 544. The analog-to-digital conversion module 543 is connected to the signal conversion module 542, collects the voltage signal, and converts the voltage signal into a digital voltage signal. The data processing module 544 is connected to the analog-to-digital conversion module 543 and configured to determine, according to the digital voltage signals before and after the detection of the sample to be detected, the resistance change value of the counting cell unit 220 before and after the detection of the sample to be detected by the counting cell unit 220, and further determine the usage amount of the cleaning solution.
In this embodiment, since the voltage signal generated by the signal conversion module 542 is an analog signal and the data processing module 544 cannot acquire and process the analog signal, the analog signal generated by the signal conversion module 542 is converted into a digital voltage signal by the analog-to-digital conversion module 543, and the digital voltage signal is provided to the data processing module 544 for signal acquisition and processing. Further, the data processing module 544 may be any data processing module capable of calculating a resistance change value. To reduce cost, in one embodiment, the data processing module 544 may be a single-chip microcomputer. Specifically, the signals collected by the data processing module 544 include the current in the cell monitoring unit 540 and the voltage across the cell monitoring unit 540. As mentioned above, taking the sample a to be tested as an example, the signal collected by the data processing module 544 includes the voltage V of the branch of the counting cell monitoring unit 540 before the blood analysis of the sample a to be tested is performedA1(ii) a After the detection of the sample A to be detected is finished, the voltage V at the two ends of the monitoring unit 540 of the detection counting cellA2. Before the blood analysis of the sample A to be measured, the counting cell monitors the current I of the branch in which the unit 540 is locatedA1(ii) a After the detection of the sample A to be detected is finished, the counting cell monitors the current I at the two ends of the unit 540A2。
Accordingly, the data processing module 544 performs operations including determining the current I of the branch of the cuvette unit 220 immediately before performing the blood analysis on the sample A to be tested11＝I-IA1And determining the current I of the branch where the counting cell unit 220 is located after the detection of the sample A to be detected is finished12＝I-IA2. The resistance R of the cell unit 220 immediately before the blood analysis of the sample A to be measured11＝VA1/I11＝VA1/(I-IA1) (ii) a Determining the resistance R of the counting cell unit 220 after the detection of the sample A to be detected is finished12＝VA2/I12＝VA2/(I-IA2). And a variation value Δ R ═ R of the resistance12-R11＝VA2/(I-IA2)-VA1/(I-IA1)。
In addition, the proper amount of the cleaning liquid is determined according to a preset relationship which is satisfied between the change value of the resistance and the amount of the cleaning liquid. For different samples to be tested, different testing items and testing conditions, the preset relationship may be a logarithmic relationship or a power relationship, and the preset relationship is not specifically limited here. In one embodiment, the predetermined relationship includes a direct proportional relationship, that is, Δ R ═ k × Q, where k is a direct proportional coefficient, Q is the amount of the cleaning solution, Δ R is the variation value of the resistance, and the direct proportional coefficient k is the experimental measurement value, and is related to the detection condition, the property of the sample to be detected, and the detection item, so the specific value thereof is not particularly limited. Specifically, the sample to be measured (blood cells) is considered to be a poor conductor, and the sample to be measured remaining in the cuvette increases the resistance value of the cuvette unit 220. Therefore, the larger the change in resistance value, the more cleaning liquid is required accordingly.
Further, referring to fig. 6, fig. 6 is a schematic structural diagram of a fourth embodiment of a cleaning solution calculating device for a blood analyzer according to the present invention, and unlike the cleaning solution calculating device for a blood analyzer in fig. 5, a counting cell monitoring unit 640 in this embodiment specifically includes a current amplifying module 641, a signal converting module 642, a signal conditioning mechanism 645, an analog-to-digital converting module 643, and a data processing module 644. In this embodiment, the signal conditioning mechanism 645 is respectively connected to the signal conversion module 642 and the analog-to-digital conversion module 643, and is configured to condition the voltage signal within the range of the analog-to-digital conversion module 643. The voltage signal is conditioned to the range of the analog-to-digital conversion module 643, which is beneficial to improving the accuracy of the voltage signal acquired by the data processing module 644, so that the usage amount of the cleaning solution is determined more accurately.
In addition, the cleaning liquid data processing module further comprises an execution mechanism which is connected with the data processing module and used for receiving the instruction sent by the data processing module and injecting the cleaning liquid with the determined using amount into the counting pool in the counting pool unit so as to clean the counting pool. Specifically, the action of the execution mechanism comprises starting a delivery pump, injecting cleaning liquid into the counting pool to clean the counting pool, and when the amount of the injected cleaning liquid reaches the injection amount determined by the data processing module, closing the delivery pump and stopping inputting the cleaning liquid into the counting pool.
In one embodiment, referring to fig. 7, fig. 7 is a schematic structural diagram of an embodiment of a blood analysis system according to the present invention, the blood analysis system 700 includes: a blood analyzer 710 and a cleaning solution calculating device 720.
The blood analyzer 710 includes a three-classification blood analyzer, a five-classification blood analyzer, and the like, and the blood analyzer 710 includes a constant current source and a counting cell unit, and the constant current source and the counting cell unit form a first loop to perform blood analysis on a sample to be detected in the counting cell unit. And the cleaning liquid calculating means 720 includes: the counting cell monitoring unit and the constant current source form a second loop to monitor the resistance change value of the sample to be tested before and after blood analysis in the counting cell unit; and the cleaning liquid calculating device correspondingly calculates and obtains the consumption of the cleaning liquid according to the monitored resistance change value.
In the present embodiment, the resistance change value of the cuvette unit before and after the blood analysis of the sample to be measured is monitored by the second circuit formed by the constant current source in the blood analyzer 710 and the cuvette monitoring unit, and the amount of the cleaning solution is obtained by the corresponding calculation based on the resistance change value monitored by the cleaning solution calculation means 720. The amount of the cleaning liquid is determined according to the change of the resistance of the counting cell before and after the sample to be detected is detected, namely the amount of the cleaning liquid is related to the amount of blood adhered to the counting cell unit, so that the cleaning effect can be guaranteed by the amount of the cleaning liquid, and the waste of the cleaning liquid is avoided.
In addition, the technical details and technical benefits of the blood analyzer 710 and the wash solution calculating device 720 have been explained in detail above and will not be described in detail here.
In another embodiment, referring to fig. 8, fig. 8 is a schematic flow chart of an embodiment of a method for calculating a cleaning solution for a blood analyzer according to the present invention, the method including the steps of:
and S810, providing any cleaning liquid calculating device.
In step S810, the cleaning solution calculating device is a cleaning solution calculating device for a blood analyzer, wherein the blood analyzer includes a constant current source and a counting cell unit, and the constant current source and the counting cell unit form a first loop to perform blood analysis on a sample to be detected in the counting cell unit; the cleaning liquid calculating device includes: the counting cell monitoring unit and the constant current source form a second loop to monitor the resistance change value of the sample to be tested before and after blood analysis in the counting cell unit; and the cleaning liquid calculating device correspondingly calculates and obtains the consumption of the cleaning liquid according to the monitored resistance change value.
Furthermore, the technical details and technical benefits of the blood analyzer and the wash solution calculating device have been explained in detail above, and will not be described in detail here
And S820, monitoring the resistance change value of the sample to be detected before and after blood analysis by the counting cell unit.
In step 820, according to the experimental results of the inventor of the present invention, it is shown that the resistance value of the cell unit and the resistance remaining in the cell satisfy a certain relationship, the amount of the sample to be measured remaining in the cell can be determined according to the change value of the resistance before and after the detection of the sample to be measured, and the appropriate amount of the cleaning solution can be determined according to the amount of the sample to be measured remaining in the cell.
And S830, correspondingly calculating and obtaining the using amount of the cleaning liquid according to the monitored resistance change value.
In step S830, the amount of the cleaning solution is determined according to the change of the resistance of the counting cell before and after detecting the sample to be detected, that is, the amount of the cleaning solution is related to the amount of the blood adhered to the counting cell unit, so that the amount of the cleaning solution can ensure the cleaning effect and avoid the waste of the cleaning solution.
Further, referring to fig. 9, fig. 9 is a flowchart illustrating an embodiment of step S820 in fig. 8, and the method further includes:
and S821, monitoring the voltage value of the sample to be detected before and after blood analysis by the counting cell unit.
In step 821, referring to fig. 2 and fig. 3 of the specification, since the counting cell monitoring unit is connected in parallel with the counting cell unit, it can be considered that the voltage of the branch in which the counting cell monitoring unit is located is the same as the voltage of the branch in which the counting cell unit is located, and the voltage values at the two ends of the counting cell unit before and after the blood analysis of the sample to be detected can be determined by monitoring the voltage at the two ends of the counting cell monitoring unit before and after the blood analysis of the sample to be detected. Specifically, the voltage V of the branch of the primary counting cell monitoring unit is detected immediately before the blood analysis of the sample A to be detectedA1(ii) a After the detection of the sample A to be detected is finished, detecting the voltage V at the two ends of the monitoring unit of the counting cell againA2。
Due to the current I passing through the branch of the counting cell monitoring unit2Much less than the current I of the branch in which the counting cell unit is located1In order to obtain the voltage at the two ends of the counting cell control unit conveniently, the current I passing through the branch of the counting cell monitoring unit is amplified by the current amplification module2The amplification is carried out, and the amplification factor can be determined according to the actual requirement. In one embodiment, the current amplifying module includes a triode, wherein a base of the triode is connected with the constant current source, and a collector of the triode is connected with the signal conversion module, that is, the amplified current flows through the signal conversion module, and the current signal is converted into a voltage signal, that is, the voltage across the monitoring unit of the counting cell is obtained.
Furthermore, because the voltage signal generated by the signal conversion module is an analog signal and the data processing module cannot acquire and process the analog signal, the analog signal generated by the signal conversion module is converted into a digital voltage signal by the analog-to-digital conversion module for the data processing module to acquire and process the signal. Further, the data processing module may be any data processing module capable of calculating a resistance change value. In order to reduce the cost, in one embodiment, the data processing module may be a single chip microcomputer. In this embodiment, the signal conditioning mechanism is connected to the signal conversion module and the analog-to-digital conversion module, respectively, and is configured to condition the voltage signal to a range of the analog-to-digital conversion module. The voltage signal is conditioned to the range of the analog-to-digital conversion module, so that the accuracy of the voltage signal acquired by the data processing module is improved, and the dosage of the cleaning liquid is determined more accurately.
In addition, the cleaning liquid data processing module further comprises an execution mechanism which is connected with the data processing module and used for receiving the instruction sent by the data processing module and injecting the cleaning liquid with the determined using amount into the counting pool in the counting pool unit so as to clean the counting pool. Specifically, the action of the execution mechanism comprises starting a delivery pump, injecting cleaning liquid into the counting pool to clean the counting pool, and when the amount of the cleaning liquid injected into the counting pool reaches the injection amount determined by the data processing module, closing the delivery pump and stopping inputting the cleaning liquid into the counting pool.
And S822, determining the current value of the counting cell units before and after the detection of the sample to be detected according to the current value of the constant current source and the current values of the counting cell monitoring units before and after the detection of the sample to be detected.
In step S822, with continuing reference to the description fig. 2 and the description fig. 3, the current value I provided by the constant current source is known, and in order to ensure that the counting cell unit needs to operate under a certain current, the current passing through the branch of the counting cell monitoring unit is far smaller than the current of the branch of the counting cell unit, that is, although the counting cell monitoring unit divides a part of the current provided by the constant current source, the current of the branch of the counting cell unit is hardly affected, and the accuracy of the counting result of the counting cell unit can be ensured. Specifically, the current I of the branch where the primary counting cell monitoring unit is located is detected immediately before blood analysis is performed on the sample A to be detectedA1Then, at this time, the current I of the branch where the counting cell unit is located11＝I-IA1(ii) a After the detection of the sample A to be detected is finished, the current I of the branch where the monitoring unit of the counting cell is located is detected againA2Then, at this time, the counting cell unit is locatedCurrent of the circuit I12＝I-IA2。
S823, determining the resistance of the counting cell unit before and after the detection of the sample to be detected according to the voltage value of the counting cell monitoring unit before and after the detection of the sample to be detected and the current value of the counting cell unit, and further determining the resistance change value.
In step S823, before coming, according to the voltage VA1And current I11Can determine the resistance R of the counting cell unit before the blood analysis of the sample A to be detected11＝VA1/I11＝VA1/(I-IA1) (ii) a In the same way, according to the voltage VA2And current I12Can determine the resistance R of the counting cell unit after the detection of the sample A to be detected is finished12＝VA2/I12＝VA2/(I-IA2). In summary, the change Δ R ═ R in resistance12-R11＝VA2/(I-IA2)-VA1/(I-IA1)。
And then, determining the proper using amount of the cleaning liquid according to the preset relation met by the change value of the resistance and the using amount of the cleaning liquid. For different samples to be tested, different testing items and testing conditions, the preset relationship may be a logarithmic relationship or a power relationship, and the preset relationship is not specifically limited here. In one embodiment, the predetermined relationship includes a direct proportional relationship, that is, Δ R ═ k × Q, where k is a direct proportional coefficient, Q is the amount of the cleaning solution, Δ R is the variation value of the resistance, and the direct proportional coefficient k is the experimental measurement value, and is related to the detection condition, the property of the sample to be detected, and the detection item, so the specific value thereof is not particularly limited. Specifically, the sample to be measured (blood cells) is considered to be a poor conductor, and the sample to be measured remaining in the cuvette increases the resistance value of the cuvette unit.
In summary, the present invention monitors the resistance change value before and after the blood analysis of the sample to be tested by the counting cell unit through the second loop formed by the constant current source in the blood analyzer and the counting cell monitoring unit, and correspondingly calculates and obtains the usage amount of the cleaning solution according to the resistance change value monitored by the cleaning solution calculating device. The amount of the cleaning liquid is determined according to the change of the resistance of the counting cell before and after the sample to be detected is detected, namely the amount of the cleaning liquid is related to the amount of blood adhered to the counting cell unit, so that the cleaning effect can be guaranteed by the amount of the cleaning liquid, and the waste of the cleaning liquid is avoided.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. A cleaning solution calculating device for a blood analyzer, wherein the blood analyzer includes a constant current source and a cuvette unit constituting a first circuit for performing blood analysis on a sample to be measured in the cuvette unit, the cleaning solution calculating device comprising:
the counting cell monitoring unit and the constant current source form a second loop to monitor the resistance change value of the counting cell unit before and after blood analysis of the sample to be detected;
the cleaning liquid calculating device correspondingly calculates and obtains the using amount of the cleaning liquid according to the monitored resistance change value; the counting cell unit works under constant current, and the current in the counting cell monitoring unit is far smaller than the current in the counting cell unit.
2. The cleaning liquid calculating apparatus as claimed in claim 1, wherein the counter monitoring unit includes:
the current amplification module is used for amplifying the current in the counting cell monitoring unit;
and the signal conversion module is connected with the current amplification module and is used for converting the amplified current signal into a voltage signal.
3. The cleaning solution calculating device according to claim 2, wherein the count tank monitoring unit further comprises:
the analog-to-digital conversion module is connected with the signal conversion module, acquires the voltage signal and converts the voltage signal into a digital voltage signal;
and the data processing module is connected with the analog-to-digital conversion module and used for determining the resistance change values of the counting cell unit before and after the counting cell unit detects the sample to be detected according to the digital voltage signals before and after the sample to be detected is detected, so as to determine the using amount of the cleaning liquid.
4. The cleaning solution calculating device according to claim 3, wherein the count tank monitoring unit further comprises:
and the signal conditioning mechanism is respectively connected with the signal conversion module and the analog-to-digital conversion module and is used for conditioning the voltage signal to the range of the analog-to-digital conversion module.
5. The cleaning liquid calculating device according to claim 3, wherein the usage amount of the cleaning liquid obtained by the corresponding calculation according to the monitored resistance change value satisfies a preset relationship, and the preset relationship includes a direct proportional relationship.
6. The cleaning solution calculating device according to claim 3, further comprising:
and the execution mechanism is connected with the data processing module and used for receiving the instruction sent by the data processing module and injecting the cleaning liquid with the determined dosage into the counting pool unit.
7. A sample analysis system comprising a blood analyzer and the cleaning solution calculation apparatus of any one of claims 1-6.
8. A method of cleaning fluid calculation for a blood analyzer, the method comprising:
providing the cleaning fluid calculation device of any of claims 1-6;
monitoring resistance change values of the counting cell unit before and after blood analysis of the sample to be detected;
and correspondingly calculating to obtain the use amount of the cleaning liquid according to the monitored resistance change value.
9. The method according to claim 8, wherein the method for monitoring the resistance change before and after the blood analysis of the sample to be tested by the counting cell unit specifically comprises:
monitoring the voltage value of the counting cell unit before and after blood analysis of the sample to be detected;
determining the current value of the counting cell unit before and after the detection of the sample to be detected according to the current value of the constant current source and the current values of the counting cell monitoring unit before and after the detection of the sample to be detected;
and determining the resistance of the counting cell unit before and after the detection of the sample to be detected according to the voltage value of the counting cell monitoring unit before and after the detection of the sample to be detected and the current value of the counting cell unit, and further determining the resistance change value.
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| CN201910361992.3A CN111855977B (en) | 2019-04-30 | 2019-04-30 | Blood analysis system, cleansing liquid calculation device, and cleansing liquid calculation method |
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| CN201910361992.3A CN111855977B (en) | 2019-04-30 | 2019-04-30 | Blood analysis system, cleansing liquid calculation device, and cleansing liquid calculation method |
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