CN104296953A - Micro hole blocking detection method, system, and blood cell analyzer - Google Patents
Micro hole blocking detection method, system, and blood cell analyzer Download PDFInfo
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- CN104296953A CN104296953A CN201310297422.5A CN201310297422A CN104296953A CN 104296953 A CN104296953 A CN 104296953A CN 201310297422 A CN201310297422 A CN 201310297422A CN 104296953 A CN104296953 A CN 104296953A
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Abstract
The invention provides a micro hole blocking detection method, a micro hole blocking detection system and a blood cell analyzer. The system includes a micro hole voltage extraction circuit which is connected with positive and negative electrodes at two sides of a micro hole of medical equipment and is used for acquiring voltage signals between the positive and negative electrodes and recording a micro hole voltage curve formed by a plurality of voltage signals, and a micro hole voltage characteristic analysis module which is connected with the micro hole voltage extraction circuit and is used for performing characteristic analysis on the voltage signals on the micro hole voltage curve and identifying whether the voltage signals mutate, and detecting whether the micro hole is blocked or not based on a time point of mutation occurrence when the micro hole voltage characteristic analysis module identifies that the voltage signals mutate. With the micro hole blocking detection method, the micro hole blocking detection system and the blood cell analyzer provided by the embodiment of the invention adopted, whether the voltage signals mutate can be accurately judged, and therefore, whether the micro hole is blocked or not can be detected based a time point of mutation occurrence.
Description
Technical field
The present invention relates to a kind of field of medical technology, particularly relate to a kind of micropore plug-hole detection method and system, blood cell analyzer of Medical Devices.
Background technology
Blood cell analyzer is commonly used Coulter principle and is carried out the counting of blood cell and hive off.In the counting chamber of analyser, there is the opening that little, be called micropore.As shown in Figure 1, there is pair of positive and negative in micropore both sides, connect constant-current supply.Due to the poor conductor that cell is electricity, the cell after dilution in sample under the effect of the pressure by detect micropore time, interelectrode resistance changes, thus forms a pulse signal be in proportion with cell volume at electrode two ends.The number of pulse is suitable with the cell number by micropore, and the amplitude of pulse is directly proportional to the volume of cell.Due to the debris accumulation of blood sample and counting chamber, micropore, can occur to detect the blocked situation of micropore in measuring process, at this moment obtain pulse height and can produce distortion, thus affect accuracy and the reliability of testing result.Therefore analyzer system must identify the situation of the change of micropore and blocking.
Method one conventional in prior art is volume metering method, namely in pipeline, increases liquid volume gage probe, judges whether there occurs plug-hole in measuring process by the situation of liquid level in-position sensor in the stipulated time.This method, owing to will increase extra pipeline, fluidic component and detecting sensor, causes the cost increase of instrument.When slight plug-hole or partial routine plug-hole occur micropore in testing process, the program cannot be found out.
Another kind method is that the height by detecting micropore voltage relatively judges plug-hole with the threshold value preset.But micropore both end voltage affects very large by factors such as electrical conductivity of solution, temperature, liquid stream and the physical parameters of micropore own, determine whether that plug-hole has very high False Rate by the method.
In order to ensure accuracy and the reliability of blood test results, be necessary to provide a kind of technology judging micropore plug-hole accurately and effectively.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of micropore plug-hole detection method and system, blood cell analyzer, accurate and effective can detect micropore plug-hole.
On the one hand, micropore plug-hole detection method provided by the invention, comprising:
Voltage signal between the positive and negative electrode of the micropore both sides of collection Medical Devices, multiple voltage signals that record gathers gained form micropore voltage curves;
Signature analysis is carried out to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation;
When recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detect micropore and whether plug-hole occurs.
Wherein, signature analysis is carried out to the voltage signal on described micropore voltage curve, identify whether voltage signal undergos mutation, and comprises the steps:
Differential process is carried out to the voltage signal on described micropore voltage curve, obtains differentiated voltage signal curve;
Analyze described differentiated voltage signal curve, identify whether this differentiated voltage signal exceedes the threshold value of setting, judge whether voltage signal corresponding to this differentiated voltage signal undergos mutation thus;
Or comprise the steps:
Described micropore voltage curve is carried out mobile staging treating;
According to voltage curve the last period of described micropore voltage curve, simulate one section of voltage curve thereafter;
The voltage curve of matching and described micropore voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus;
Or comprise the steps:
Detect the voltage signal without the micropore of plug-hole, obtain standard microwell voltage curve;
Described micropore voltage curve and described standard microwell voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus.
Wherein, when recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detect micropore and whether plug-hole occur, comprising:
Judge whether the moment that described sudden change occurs suddenlys change within the scope of the moment in precognition, if not, judges described micropore generation plug-hole.
Wherein, when recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, after whether detection micropore plug-hole occurs, also comprise:
According to the moment of described micropore plug-hole, carry out reporting to the police and/or follow-up unimpeded process.
On the other hand, a kind of micropore plug-hole detection system provided by the invention, comprising:
Micropore voltage extraction circuit, it connects the positive and negative electrode of the micropore both sides of Medical Devices, and for gathering the voltage signal between positive and negative electrode, and multiple voltage signals that record gathers gained form micropore voltage curve;
Micropore voltage characteristic analysis module, it is connected with described micropore voltage extraction circuit, for carrying out signature analysis to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation; When recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detect micropore and whether plug-hole occurs.
Wherein, described micropore voltage characteristic analysis module, comprising:
Differential processing unit, for carrying out differential process to the voltage signal on described micropore voltage curve, obtains differentiated voltage signal curve;
First Signal analysis unit, for analyzing described differentiated voltage signal curve, identifies whether this differentiated voltage signal exceedes the threshold value of setting, judges whether voltage signal corresponding to this differentiated voltage signal undergos mutation thus.
Wherein, described micropore voltage characteristic analysis module, comprising:
Staging treating unit, for carrying out mobile staging treating by described micropore voltage curve;
Curve unit, for voltage curve the last period according to described micropore voltage curve, simulates one section of voltage curve thereafter;
Secondary signal recognition unit, for the voltage curve of described curve unit matching and described micropore voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus.
Wherein, described micropore voltage characteristic analysis module, comprising:
Typical curve generation unit, for detecting the voltage signal of the micropore without plug-hole, obtains standard microwell voltage curve;
3rd Signal analysis unit, is analyzed for just described micropore voltage curve and described standard microwell voltage curve, Recognition Different signal, and judges whether voltage signal undergos mutation thus.
Wherein, described system also comprises:
Plug-hole determination module, for judging whether the moment that described sudden change occurs suddenlys change within the scope of the moment in precognition, if not, judges described micropore generation plug-hole.
Wherein, described system also comprises:
Processing module, for the moment according to described micropore plug-hole, carries out reporting to the police and/or follow-up unimpeded process.
The present invention also provides a kind of blood cell analyzer, it is characterized in that, comprising: counting chamber, micropore and constant current source, and aforesaid micropore plug-hole detection system; Be full of conductive solvent in described counting chamber, and be divided into forebay and after-bay by described micropore; Be respectively arranged with a positive electrode and a negative electrode in forebay and after-bay, this positive and negative electrode is connected with one end of constant current source; Described micropore plug-hole detection system connects the positive electrode and negative electrode that arrange in described forebay and after-bay respectively.
Implement the present invention, due to micropore voltage extraction circuit is directly carried in detect micropore both sides positive and negative polarities between, can acquisition testing micropore both end voltage change accurately, and signature analysis is carried out to the voltage signal collected, accurately can judge whether voltage signal undergos mutation, and the moment detection combining sudden change generation draws micropore plug-hole, compared to existing technologies, effect of the present invention: one is that accuracy of judgement is effective, is not subject to other factors impact; Two is can judge to count to start the front plug-hole phenomenon just occurred.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of blood cell analyzer in prior art;
Fig. 2 is the structural representation of blood cell analyzer provided by the invention;
Fig. 3 is a structural representation of micropore voltage extraction circuit in micropore plug-hole detection system provided by the invention;
Fig. 4 is the another structural representation of micropore voltage extraction circuit in micropore plug-hole detection system provided by the invention;
Fig. 5 is the structural representation of micropore voltage characteristic analysis module embodiment one in micropore plug-hole detection system provided by the invention;
Fig. 6 is a schematic diagram of micropore voltage curve of the present invention and micropore voltage derivative curve;
Fig. 7 is the another schematic diagram of micropore voltage curve of the present invention and micropore voltage derivative curve;
Fig. 8 is the another schematic diagram of micropore voltage curve of the present invention and micropore voltage derivative curve;
Fig. 9 is the another schematic diagram of micropore voltage curve of the present invention and micropore voltage derivative curve;
Figure 10 is the structural representation of micropore voltage characteristic analysis module embodiment two in micropore plug-hole detection system provided by the invention;
Figure 11 is the schematic diagram of matching micropore voltage curve of the present invention;
Figure 12 is the schematic diagram of micropore voltage curve under plug-hole state of the present invention;
Figure 13 is the structural representation of micropore voltage characteristic analysis module embodiment three in micropore plug-hole detection system provided by the invention;
Figure 14 is the schematic diagram of standard microwell voltage curve of the present invention;
Figure 15 is the another schematic diagram of micropore voltage curve under plug-hole state of the present invention;
Figure 16 is the overall procedure schematic diagram of micropore plug-hole detection method provided by the invention;
Figure 17 is the schematic flow sheet of micropore plug-hole detection method embodiment one provided by the invention;
Figure 18 is the schematic flow sheet of micropore plug-hole detection method embodiment two provided by the invention;
Figure 19 is the schematic flow sheet of micropore plug-hole detection method embodiment three provided by the invention.
Embodiment
The present invention acquires the voltage at the micropore two ends of Medical Devices first accurately, then its variation characteristic is analyzed, whether detectable voltage signals is undergone mutation and is combined the moment of sudden change, thus the impact of extraneous factor is foreclosed, and can detect micropore plug-hole accurately.Implementation procedure of the present invention will be introduced in detail below.
It should be noted that, the Medical Devices that the present invention mentions can be blood cell analyzers, also can be that other have the Medical Devices for the micropore detected, as long as its Cleaning Principle is that cell is passed through micropore under the effect of the pressure, interelectrode resistance changes, thus forming one at electrode two ends with the directly proportional pulse signal of cell volume size, then the present invention is applicable to the detection of this kind of micropore plug-hole.
For blood analyser, see Fig. 2, for the invention provides a kind of structural representation of blood analyser, it comprises micropore plug-hole detection system and detects for carrying out cell the counting chamber analyzed, be full of conductive solvent in this counting chamber, and be divided into forebay and after-bay by micropore; Be respectively arranged with a positive electrode and a negative electrode in forebay and after-bay, this positive and negative electrode is connected with one end of constant current source, the electrode arranged in forebay, conductive solvent, the electrode arranged in after-bay, and constant current source forms the closed-loop path of a series connection together; This micropore plug-hole detection system is connected between described positive and negative electrode.
Micropore plug-hole detection system provided by the invention, comprising:
Micropore voltage extraction circuit 1, it connects the positive and negative electrode of micropore both sides of Medical Devices, and for the voltage signal between the positive and negative electrode of micropore both sides that gathers, and multiple voltage signals that record gathers gained form micropore voltage curves;
Micropore voltage characteristic analysis module 2, it is connected with described micropore voltage extraction circuit 1, for carrying out signature analysis to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation; When recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detect micropore and whether plug-hole occurs.
Preferred embodiment, described system also comprises:
Plug-hole situation determination module 3, for judging whether the moment that described sudden change occurs suddenlys change within the scope of the moment in precognition, if not, judges described micropore generation plug-hole;
Concrete, precognition sudden change moment scope refers to and is powering on or the lower electricity moment, or some specific moment, voltage signal can be undergone mutation, within these precognition sudden change moment, voltage signal is undergone mutation, do not belong to the voltage jump that plug-hole causes, the voltage signal sudden change occurred outside precognition sudden change moment scope, then can think to be caused by plug-hole.
Processing module 4, for the moment according to described micropore plug-hole, carries out reporting to the police and/or follow-up unimpeded process.
In specific implementation, record the moment that above-mentioned voltage signal is undergone mutation, if this moment is not within the above-mentioned precognition sudden change moment, then represent micropore generation plug-hole, start alert event and carry out alarm, the content of this warning message mainly comprises micropore generation plug-hole, can also comprise the moment that voltage signal is undergone mutation further.Further, follow-up unimpeded process can also be carried out, comprise and operation is cleaned etc. to micropore.
Adopt micropore plug-hole detection system provided by the invention, its micropore voltage extraction circuit is directly carried on the positive and negative electrode of detection micropore both sides, the change in voltage at micropore two ends can be detected accurately, based on the voltage signal that this micropore voltage extraction circuit collects, signature analysis can be carried out, identify the moment whether voltage signal is undergone mutation and suddenlyd change, to identify micropore plug-hole accurately and effectively.
In specific implementation, this micropore voltage extraction circuit can be as shown in Figure 3, this micropore voltage extraction circuit is connected between the positive and negative polarities of micropore both sides, concrete: between the positive and negative polarities by a micropore sensor connection micropore, this micropore sensor one end is connected with the base stage of triode Q1, the other end is connected by the emitter of resistance with triode Q1, this triode Q1 is for amplifying the voltage signal between these detection micropore the two poles of the earth, the emitter of this triode Q1 also connects a signal conditioning circuit, and it is for adjusting the micropore voltage signal amplified through triode Q1; Certainly this micropore voltage extraction circuit is only example, and it can also have other equivalent way of realization.
In addition, when the voltage utilizing micropore voltage extraction circuit to micropore two ends gathers, a resistance can also be added between the positive and negative electrode of micropore, specifically as shown in Figure 4.Through the micropore voltage signal that the micropore voltage extraction circuit shown in Fig. 4 is extracted.
Collect utilizing micropore voltage extraction circuit and detect the voltage signal at micropore two ends, and record this voltage signal with after forming micropore voltage curve, signature analysis can be carried out to this micropore voltage curve, accurately to judge micropore plug-hole.In specific implementation, there is following several mode can realize accurately judging micropore plug-hole.
First kind of way carries out differential process to the voltage signal on described micropore voltage curve to carry out plug-hole identification.
Undertaken in the mode of plug-hole identification by carrying out differential process to voltage signal, as shown in Figure 5, described micropore voltage characteristic analysis module specifically comprises:
Differential processing unit 20, for carrying out differential process to the voltage signal on described micropore voltage curve, obtains differentiated voltage signal curve; Concrete, carrying out differential process to voltage signal also has multiple concrete way of realization, such as: carry out digital signal processing to the voltage signal that micropore voltage extraction circuit is extracted, obtain differentiated voltage signal; Or by hardware circuit, voltage signal is become differentiated voltage signal etc.
First Signal analysis unit 21, for analyzing described differentiated voltage signal curve, for analyzing described differentiated voltage signal curve, identifying whether this differentiated voltage signal exceedes the threshold value of setting, judging whether voltage signal corresponding to this differentiated voltage signal undergos mutation thus.
It should be noted that, carry out differential process to the voltage signal on micropore voltage curve, practical function is the rate of change of amplification voltage signal.When not there is plug-hole in micropore, voltage signal can not be undergone mutation, concrete as Fig. 6, when micropore generation plug-hole situation, because plug-hole is a process for sudden change, therefore by carrying out differential process to voltage signal, just whether can undergo mutation according to voltage signal and combine sudden change moment detect plug-hole.
If there is plug-hole in the process counted through micropore cell, its original voltage curve and voltage derivative signal curve are as shown in Figure 7 and Figure 8, if just there is plug-hole before counting, its original voltage curve and voltage derivative signal curve are then as shown in Figure 9.Significantly can find out the voltage signal producing sudden change according to Fig. 7 ~ Fig. 9, namely the moment that the voltage signal of this sudden change is corresponding is the moment that plug-hole occurs, and the amplitude of the voltage signal of this sudden change can represent the degree of plug-hole.
The second way carries out curve fitting to micropore voltage curve to carry out plug-hole identification.
As shown in Figure 10, described micropore voltage characteristic analysis module, comprising this mode:
Staging treating unit 22, for carrying out mobile staging treating by described micropore voltage curve;
Curve unit 23, for voltage curve the last period according to described micropore voltage curve, simulates one section of voltage curve thereafter;
Secondary signal recognition unit 24, for the voltage curve of described curve unit 23 matching and described micropore voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus.
Figure 11 and Figure 12 is that example is described, normal micropore voltage curve should be as shown in figure 11, when counting, its curve should be level and smooth and level off to straight line, therefore can simulate rear one section of micropore voltage curve under normal circumstances according to curve the last period of this micropore voltage signal curve.And then actual for the such as Figure 12 micropore voltage curve 11 detecting micropore voltage signal curve and the matching obtained is compared, can identify the voltage signal occurring difference, this occurs that namely the voltage signal of difference represents that voltage signal is undergone mutation.
The third mode is compared by the micropore voltage curve of micropore voltage curve and standard, to carry out plug-hole identification.
As shown in figure 13, described micropore voltage characteristic analysis module, comprising this mode:
Typical curve generation unit 25, forms standard microwell voltage curve for the voltage signal that detects without the micropore of plug-hole;
3rd Signal analysis unit 26, is analyzed for the micropore voltage curve that generated by described typical curve generation unit 25 and described standard microwell voltage curve, Recognition Different signal, and judges whether voltage signal undergos mutation thus.
In specific implementation, typical curve generation unit 25 goes out the micropore voltage curve of a standard according to the micropore voltage statistic normally without plug-hole, as shown in figure 14, and using this micropore voltage curve as a template, then the detection micropore voltage curve obtained as Figure 15 is carried out mating or comparative analysis with this curve module, if mate unsuccessful or correlativity is lower, this occurs that namely the voltage signal of difference represents that voltage signal is undergone mutation.
Accordingly, the present invention also provides micropore plug-hole detection method, as shown in figure 16, comprising:
Step 100, the voltage signal between the positive and negative electrode of the detection micropore both sides of collection Medical Devices, multiple voltage signals that record gathers gained form micropore voltage curves;
Step 101, carries out signature analysis to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation.
Step 102, when recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detects micropore and whether plug-hole occurs.
Concrete, judge whether the moment that described sudden change occurs suddenlys change within the scope of the moment in precognition, if not, judges described micropore generation plug-hole.Precognition sudden change moment scope refers to and is powering on or the lower electricity moment, or some specific moment, voltage signal can be undergone mutation, the voltage signal of the sudden change occurred within these precognition sudden change moment, do not belong to the voltage jump that plug-hole causes, the voltage signal sudden change occurred outside precognition sudden change moment scope, then can think to be caused by plug-hole.
Step 103, according to the moment of described micropore plug-hole, reports to the police;
Step 104, according to the moment of described micropore plug-hole, carries out follow-up unimpeded process.
In specific implementation, record the moment that above-mentioned voltage signal is undergone mutation, if this moment is not within the above-mentioned precognition sudden change moment, then represent micropore generation plug-hole, start alert event and carry out alarm, the content of this warning message mainly comprises micropore generation plug-hole, can also comprise the moment that voltage signal is undergone mutation further.Further, follow-up unimpeded process can also be carried out, comprise and operation is cleaned etc. to micropore.
Collecting the voltage signal detecting micropore two ends, and record this voltage signal with after forming micropore voltage curve, signature analysis can be carried out to this micropore voltage curve, identify the moment whether voltage signal is undergone mutation and suddenlyd change, accurately to judge micropore plug-hole.In specific implementation, there is following several mode can realize accurately judging micropore plug-hole.
First kind of way carries out differential process to the voltage signal on described micropore voltage curve to carry out plug-hole identification.
As shown in figure 17, first kind of way specifically comprises:
Step 200, carries out differential process to the voltage signal on described micropore voltage curve, obtains differentiated voltage signal curve;
Step 201, analyzes described differentiated voltage signal curve, identifies whether this differentiated voltage signal exceedes the threshold value of setting, judges whether voltage signal corresponding to this differentiated voltage signal undergos mutation thus.
It should be noted that, carry out differential process to the voltage signal on micropore voltage curve, practical function is the rate of change of amplification voltage signal.When not there is plug-hole in detection micropore, voltage signal can not be undergone mutation, concrete as Fig. 6, when detecting micropore generation plug-hole situation, because plug-hole is a process for sudden change, therefore by carrying out differential process to voltage signal, just plug-hole moment and degree can be detected according to the voltage signal of sudden change.
If there is plug-hole in the process counted through micropore cell, its original voltage curve and voltage derivative signal curve are as shown in Figure 7 and Figure 8, if just there is plug-hole before counting, its original voltage curve and voltage derivative signal curve are then as shown in Figure 9.Significantly can find out the voltage signal producing sudden change according to Fig. 7 ~ Fig. 9, namely the moment that the voltage signal of this sudden change is corresponding is the moment that plug-hole occurs, and the amplitude of the voltage signal of this sudden change can represent state or the degree of plug-hole.
The second way carries out curve fitting to micropore voltage curve to carry out plug-hole identification.
As shown in figure 18, the second way comprises:
Step 300, carries out mobile staging treating by described micropore voltage curve;
Step 301, according to voltage curve the last period of described micropore voltage curve, simulates one section of voltage curve thereafter;
Step 302, is analyzed the voltage curve of matching and described micropore voltage curve, Recognition Different signal, and judges whether voltage signal undergos mutation thus.
Figure 11 and Figure 12 is that example is described, normal micropore voltage curve should be as shown in figure 14, when counting, its curve should be level and smooth and level off to straight line, therefore can simulate rear one section of micropore voltage curve under normal circumstances according to curve the last period of this micropore voltage signal curve.And then actual for the such as Figure 12 micropore voltage curve 11 detecting micropore voltage signal curve and the matching obtained is compared, can identify the voltage signal occurring difference, this occurs that namely the voltage signal of difference represents that voltage signal is undergone mutation.
The third mode is compared by the micropore voltage curve of micropore voltage curve and standard, to carry out plug-hole identification.
This mode as shown in figure 19, carries out signature analysis to the voltage signal on described micropore voltage curve, identifies the voltage signal producing sudden change, comprising:
Step 400, detects the voltage signal without the micropore of plug-hole and forms standard microwell voltage curve;
Step 401, is analyzed described micropore voltage curve and described standard microwell voltage curve, Recognition Different signal, and judges whether voltage signal undergos mutation thus.
In specific implementation, the micropore voltage curve of a standard is gone out according to the micropore voltage statistic normally without plug-hole, as shown in figure 14, and using this micropore voltage curve as a template, then the detection micropore voltage curve obtained as Figure 15 is carried out mating or comparative analysis with this curve module, if mate unsuccessful or correlativity is lower, this occurs that namely the voltage signal of difference represents that voltage signal is undergone mutation.
Implement the present invention, its beneficial effect is as follows:
First, if use the high-low threshold value of the micropore voltage preset to carry out the method for plug-hole judgement, because its influence factor is more, as the discreteness of micropore device, between causing batch even batch in different machines have larger difference, so the accurate setting of threshold value is a very difficult thing, be easy to erroneous judgement occurs or fails to judge; And micropore plug-hole detection system provided by the invention, its micropore voltage extraction circuit is carried on the positive and negative electrode of detection micropore both sides, acquire micropore voltage accurately, then the variation characteristic of voltage signal is analyzed, the impact of extraneous factor is weakened from these two aspects, be exaggerated the feature of plug-hole, thus on plug-hole judges accurately and reliably.Original signal changes less obvious plug-hole situation, uses this method also it can be identified exactly.
Second, micropore impedance method is used to count in prior art, need to wait for that micropore powers on and just start counting after a period of stabilisation, if so the Using statistics unit interval by the method for particle with pore number, then the situation that plug-hole occurs before starting counting just can not accurately identify; And the present invention monitored micropore power on after full range voltage, then by counting open-interval limit, decrease the possibility of failing to judge.
3rd, the present invention, while identifying plug-hole phenomenon accurately, can also get the moment that its plug-hole occurs exactly, can assist the differentiation to plug-hole state, so that the subsequent treatment of cellanalyzer.
Therefore, the present invention is classic method comparatively, and one is that accuracy of judgement is effective, is not subject to other factors impact; Two is can judge to count to start the front plug-hole phenomenon just occurred; Three is to identify plug-hole temporal information, strengthens the judgement to plug-hole state.
One of ordinary skill in the art will appreciate that all or part of flow process realized in above-described embodiment method, that the hardware that can carry out instruction relevant by computer program has come, described program can be stored in a computer read/write memory medium, this program, when performing, can comprise the flow process of the embodiment as above-mentioned each side method.Wherein, described storage medium can be magnetic disc, CD, read-only store-memory body (Read-Only Memory, ROM) or random store-memory body (Random Access Memory, RAM) etc.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (11)
1. a micropore plug-hole detection method, is characterized in that, comprising:
Voltage signal between the positive and negative electrode of the micropore both sides of collection Medical Devices, multiple voltage signals that record gathers gained form micropore voltage curves;
Signature analysis is carried out to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation;
When recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detect micropore and whether plug-hole occurs.
2. micropore plug-hole detection method as claimed in claim 1, is characterized in that, carry out signature analysis to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation, and comprises the steps:
Differential process is carried out to the voltage signal on described micropore voltage curve, obtains differentiated voltage signal curve;
Analyze described differentiated voltage signal curve, identify whether this differentiated voltage signal exceedes the threshold value of setting, judge whether voltage signal corresponding to this differentiated voltage signal undergos mutation thus;
Or comprise the steps:
Described micropore voltage curve is carried out mobile staging treating;
According to voltage curve the last period of described micropore voltage curve, simulate one section of voltage curve thereafter;
The voltage curve of matching and described micropore voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus;
Or comprise the steps:
Detect the voltage signal without the micropore of plug-hole, obtain standard microwell voltage curve;
Described micropore voltage curve and described standard microwell voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus.
3. micropore plug-hole detection method as claimed in claim 2, is characterized in that, when recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detects micropore and whether plug-hole occurs, comprising:
Judge whether the moment that described sudden change occurs suddenlys change within the scope of the moment in precognition, if not, judges described micropore generation plug-hole.
4. micropore plug-hole detection method as claimed in claim 3, is characterized in that, when recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, after whether detection micropore plug-hole occurs, also comprises:
According to the moment of described micropore plug-hole, carry out reporting to the police and/or follow-up unimpeded process.
5. a micropore plug-hole detection system, is characterized in that, comprising:
Micropore voltage extraction circuit, it connects the positive and negative electrode of the micropore both sides of Medical Devices, and for gathering the voltage signal between positive and negative electrode, and multiple voltage signals that record gathers gained form micropore voltage curve;
Micropore voltage characteristic analysis module, it is connected with described micropore voltage extraction circuit, for carrying out signature analysis to the voltage signal on described micropore voltage curve, identifies whether voltage signal undergos mutation; When recognizing described voltage signal and undergoing mutation, in conjunction with the moment that sudden change occurs, detect micropore and whether plug-hole occurs.
6. micropore plug-hole detection system as claimed in claim 5, it is characterized in that, described micropore voltage characteristic analysis module, comprising:
Differential processing unit, for carrying out differential process to the voltage signal on described micropore voltage curve, obtains differentiated voltage signal curve;
First Signal analysis unit, for analyzing described differentiated voltage signal curve, identifies whether this differentiated voltage signal exceedes the threshold value of setting, judges whether voltage signal corresponding to this differentiated voltage signal undergos mutation thus.
7. micropore plug-hole detection system as claimed in claim 5, it is characterized in that, described micropore voltage characteristic analysis module, comprising:
Staging treating unit, for carrying out mobile staging treating by described micropore voltage curve;
Curve unit, for voltage curve the last period according to described micropore voltage curve, simulates one section of voltage curve thereafter;
Secondary signal recognition unit, for the voltage curve of described curve unit matching and described micropore voltage curve are analyzed, Recognition Different signal, and judge whether voltage signal undergos mutation thus.
8. micropore plug-hole detection system as claimed in claim 5, it is characterized in that, described micropore voltage characteristic analysis module, comprising:
Typical curve generation unit, for detecting the voltage signal of the micropore without plug-hole, obtains standard microwell voltage curve;
3rd Signal analysis unit, is analyzed for just described micropore voltage curve and described standard microwell voltage curve, Recognition Different signal, and judges whether voltage signal undergos mutation thus.
9. the micropore plug-hole detection system according to any one of claim 6 to 8, it is characterized in that, described system also comprises:
Plug-hole determination module, for judging whether the moment that described sudden change occurs suddenlys change within the scope of the moment in precognition, if not, judges described micropore generation plug-hole.
10. micropore plug-hole detection system as claimed in claim 9, it is characterized in that, described system also comprises:
Processing module, for the moment according to described micropore plug-hole, carries out reporting to the police and/or follow-up unimpeded process.
11. 1 kinds of blood cell analyzers, is characterized in that, comprising: counting chamber, micropore and constant current source, and a kind of micropore plug-hole detection system according to any one of claim 5 to 10; Be full of conductive solvent in described counting chamber, and be divided into forebay and after-bay by described micropore; Be respectively arranged with a positive electrode and a negative electrode in forebay and after-bay, this positive and negative electrode is connected with one end of constant current source; Described micropore plug-hole detection system connects the positive electrode and negative electrode that arrange in described forebay and after-bay respectively.
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