CN104296953B - A kind of micropore plug-hole detection method and system, blood cell analyzer - Google Patents
A kind of micropore plug-hole detection method and system, blood cell analyzer Download PDFInfo
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- CN104296953B CN104296953B CN201310297422.5A CN201310297422A CN104296953B CN 104296953 B CN104296953 B CN 104296953B CN 201310297422 A CN201310297422 A CN 201310297422A CN 104296953 B CN104296953 B CN 104296953B
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Abstract
The present invention provides a kind of micropore plug-hole detection method and system, blood cell analyzer, and the system includes：Micropore voltage extraction circuit, its connect the positive and negative electrode of the micropore both sides of Medical Devices, for gathering the voltage signal between positive and negative electrode, and record multiple voltage signals formation micropore voltage curves of collection gained；Micropore voltage characteristic analysis module, its are connected with the micropore voltage extraction circuit, and for carrying out signature analysis to the voltage signal on the micropore voltage curve, whether identification voltage signal undergos mutation；When recognizing the voltage signal and undergoing mutation, whether the moment that binding mutation occurs, detection micropore there is plug-hole.Implement the present invention, can accurately judge whether voltage signal undergos mutation, and the moment of binding mutation, so as to detect micropore plug-hole.
Description
Technical field
The present invention relates to a kind of field of medical technology, more particularly to a kind of Medical Devices micropore plug-hole detection method and be
System, blood cell analyzer.
Background technology
The conventional Coulter principle of blood cell analyzer carries out the counting of blood cell and a point group.Counting chamber in analyzer
In have a little opening, be called micropore.As shown in figure 1, having a pair of positive and negative in micropore both sides, connect constant-current supply.By
In cell be electricity non-conductor, the cell after dilution in sample under the effect of the pressure by detect micropore when, between electrode
Resistance change, so as to electrode two ends formed a pulse signal being in proportion with cell volume.Pulse
Number is suitable with by the cell number of micropore, and the amplitude of pulse is directly proportional to the volume of cell.Due to blood sample and counting chamber,
The debris accumulation of micropore, can occur in measurement process to detect the situation that micropore is blocked, at this moment obtaining impulse amplitude can produce
Distortion, so that affect the accuracy and reliability of testing result.Therefore analyzer system must be to the change of micropore and blocking
Situation is identified.
Method commonly used in the prior art one is volume metering method, i.e., in pipeline increase liquid volume gage probe,
Judge whether plug-hole is there occurs in measurement process by the situation of liquid level in-position sensor in the stipulated time.This method
As extra pipeline, fluidic component and detection sensor will be increased, cause the cost increase of instrument.When micropore is in detection process
Middle when there is slight plug-hole or partial routine plug-hole, the program cannot be found out.
Another kind of method is to judge plug-hole by detecting the height of micropore voltage and threshold value set in advance relatively.But
Micropore both end voltage is affected very greatly, by this side by factors such as electrical conductivity of solution, temperature, liquid stream and micropore physical parameters itself
Method determines whether that plug-hole has very high False Rate.
In order to ensure the accuracy and reliability of blood test results, it is necessary to provide one kind and accurately and effectively judge micropore
The technology of plug-hole.
Content of the invention
For solving above-mentioned technical problem, the present invention provides a kind of micropore plug-hole detection method and system, blood cell analysis
Instrument, can accurate and effective detection micropore plug-hole.
On the one hand, the micropore plug-hole detection method that the present invention is provided, including：
Voltage signal between the positive and negative electrode of the micropore both sides of collection Medical Devices, multiple voltages of record collection gained
Signal forms micropore voltage curve；
Signature analysis is carried out to the voltage signal on the micropore voltage curve, and whether identification voltage signal undergos mutation；
When recognizing the voltage signal and undergoing mutation, whether the moment that binding mutation occurs, detection micropore block up
Hole, including：Judge whether the moment that the mutation occurs is being predicted in the range of the mutation moment, if it is not, judging that the micropore occurs
Plug-hole.
Wherein, signature analysis is carried out to the voltage signal on the micropore voltage curve, and whether identification voltage signal occurs
Mutation, comprises the steps：
Differential process are carried out to the voltage signal on the micropore voltage curve, obtain differentiated voltage signal curve；
The differentiated voltage signal curve is analyzed, recognizes whether the differentiated voltage signal exceedes the threshold value for setting, thus sentenced
Whether the disconnected corresponding voltage signal of the differentiated voltage signal undergos mutation；
Or comprise the steps：
The micropore voltage curve is moved segment processing；
According to the last period voltage curve of the micropore voltage curve, one section of voltage curve thereafter is fitted；
The voltage curve of fitting is analyzed with the micropore voltage curve, Recognition Different signal, and is thus sentenced
Whether disconnected voltage signal undergos mutation；
Or comprise the steps：
The voltage signal of micropore of the detection without plug-hole, obtains standard microwell voltage curve；
The micropore voltage curve is analyzed with the standard microwell voltage curve, Recognition Different signal, and
Thus judge whether voltage signal undergos mutation.
Wherein, when recognizing the voltage signal and undergoing mutation, whether the moment that binding mutation occurs, detection micropore are sent out
After raw plug-hole, also include：
According to the moment of the micropore plug-hole, reported to the police and/or follow-up unimpeded is processed.
On the other hand, a kind of micropore plug-hole detecting system that the present invention is provided, including：
Micropore voltage extraction circuit, its connect the positive and negative electrode of the micropore both sides of Medical Devices, for gathering positive and negative electrode
Between voltage signal, and record the multiple voltage signals of collection gained and form micropore voltage curves；
Micropore voltage characteristic analysis module, its are connected with the micropore voltage extraction circuit, for the micropore voltage
Voltage signal on curve carries out signature analysis, and whether identification voltage signal undergos mutation；Send out the voltage signal is recognized
During raw mutation, whether the moment that binding mutation occurs, detection micropore there is plug-hole；
Wherein, the system also includes：
Plug-hole determining module, for judging the moment of the mutation generation whether in the range of precognition is mutated the moment, if it is not,
Judge that the micropore occurs plug-hole.
Wherein, the micropore voltage characteristic analysis module, including：
Differential process unit, for carrying out differential process to the voltage signal on the micropore voltage curve, obtains differential
Voltage signal curve；
Whether first signal identification unit, for analyzing the differentiated voltage signal curve, recognize the differentiated voltage signal
Exceed the threshold value for setting, thus judge whether the corresponding voltage signal of the differentiated voltage signal undergos mutation.
Wherein, the micropore voltage characteristic analysis module, including：
Segment processing unit, for moving segment processing by the micropore voltage curve；
Curve matching unit, for the last period voltage curve according to the micropore voltage curve, fits one section thereafter
Voltage curve；
Secondary signal recognition unit, the voltage curve and the micropore voltage for the curve matching unit to be fitted are bent
Line is analyzed, Recognition Different signal, and thus judges whether voltage signal undergos mutation.
Wherein, the micropore voltage characteristic analysis module, including：
Calibration curve signal generating unit, for the voltage signal of micropore of the detection without plug-hole, obtains standard microwell voltage curve；
3rd signal identification unit, it is right to carry out for just described micropore voltage curve and the standard microwell voltage curve
Than analysis, Recognition Different signal, and thus judge whether voltage signal undergos mutation.
Wherein, the system also includes：
Processing module, for the moment according to the micropore plug-hole, is reported to the police and/or follow-up unimpeded is processed.
The present invention also provides a kind of blood cell analyzer, it is characterised in that include：Counting chamber, micropore and constant-current source, with
And aforesaid micropore plug-hole detecting system；Be full of conductive solvent in the counting chamber, and by the micropore be divided into forebay and after
Pond；A positive electrode and a negative electrode is respectively arranged with forebay and after-bay, and the positive and negative electrode is connected with one end of constant-current source；
The micropore plug-hole detecting system connects the positive electrode and negative electrode arranged in the forebay and after-bay respectively.
Implement the present invention, due to by micropore voltage extraction circuit be loaded directly into detection micropore both sides positive and negative polarities it
Between, can accurately acquisition testing micropore both end voltage change, and signature analysis is carried out to the voltage signal for collecting, can be with
Accurately judge whether voltage signal undergos mutation, and the moment detection that binding mutation occurs draws micropore plug-hole, compares existing
For technology, the effect of the present invention：One is that accuracy of judgement is effective, is difficult to be affected by other factorses；Two is to may determine that counting
The plug-hole phenomenon just occurred before starting.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of blood cell analyzer in prior art；
The structural representation of the blood cell analyzer that Fig. 2 is provided for the present invention；
A structural representation of micropore voltage extraction circuit in the micropore plug-hole detecting system that Fig. 3 is provided for the present invention；
The another structural representation of micropore voltage extraction circuit in the micropore plug-hole detecting system that Fig. 4 is provided for the present invention；
The structure of micropore voltage characteristic analysis module embodiment one in the micropore plug-hole detecting system that Fig. 5 is provided for the present invention
Schematic diagram；
Fig. 6 is a schematic diagram of the micropore voltage curve and micropore voltage derivative curve of the present invention；
Fig. 7 is the another schematic diagram of the micropore voltage curve and micropore voltage derivative curve of the present invention；
Fig. 8 is the another schematic diagram of the micropore voltage curve and micropore voltage derivative curve of the present invention；
Fig. 9 is the another schematic diagram of the micropore voltage curve and micropore voltage derivative curve of the present invention；
The knot of micropore voltage characteristic analysis module embodiment two in the micropore plug-hole detecting system that Figure 10 is provided for the present invention
Structure schematic diagram；
Figure 11 is the schematic diagram of the fitting micropore voltage curve of the present invention；
Figure 12 is the schematic diagram of micropore voltage curve under plug-hole state of the invention；
The knot of micropore voltage characteristic analysis module embodiment three in the micropore plug-hole detecting system that Figure 13 is provided for the present invention
Structure schematic diagram；
Figure 14 is the schematic diagram of the standard microwell voltage curve of the present invention；
Figure 15 is the another schematic diagram of micropore voltage curve under plug-hole state of the present invention；
The overall procedure schematic diagram of the micropore plug-hole detection method that Figure 16 is provided for the present invention；
The schematic flow sheet of the micropore plug-hole detection method embodiment one that Figure 17 is provided for the present invention；
The schematic flow sheet of the micropore plug-hole detection method embodiment two that Figure 18 is provided for the present invention；
The schematic flow sheet of the micropore plug-hole detection method embodiment three that Figure 19 is provided for the present invention.
Specific embodiment
The present invention accurately acquires the voltage at the micropore two ends of Medical Devices first, then analyzes its variation characteristic, examines
Survey voltage signal whether undergo mutation and binding mutation moment, so as to the impact of extraneous factor is foreclosed, can be accurate
Detection micropore plug-hole.Hereinafter will be described in detail the present invention realizes process.
It should be noted that the Medical Devices that the present invention is referred to can be blood cell analyzer, or other tools
There are the Medical Devices for the micropore for detecting, as long as its Cleaning Principle is under the effect of the pressure by micropore, electrode by cell
Between resistance change, so as to form one with the directly proportional pulse signal of cell volume size at electrode two ends, then this
Invention is suitable for the detection of this kind of micropore plug-hole.
By taking blood analyser as an example, referring to Fig. 2, a kind of structural representation of blood analyser is provided for the present invention, which wraps
Include in micropore plug-hole detecting system and the counting chamber for being tested and analyzed to cell, the counting chamber and be full of conductive solvent,
And forebay and after-bay are divided into by micropore；A positive electrode and a negative electrode is respectively arranged with forebay and after-bay, and this is positive and negative
Electrode is connected with one end of constant-current source, the electrode arranged in forebay, conductive solvent, the electrode arranged in after-bay, and constant-current source
The closed-loop path of a series connection is constituted together；The micropore plug-hole detecting system is connected between the positive and negative electrode.
The micropore plug-hole detecting system that the present invention is provided, including：
Micropore voltage extraction circuit 1, its connect the positive and negative electrode of the micropore both sides of Medical Devices, for the micropore two for gathering
Voltage signal between the positive and negative electrode of side, and record multiple voltage signals formation micropore voltage curves of collection gained；
Micropore voltage characteristic analysis module 2, its are connected with the micropore voltage extraction circuit 1, for micropore electricity
The voltage signal that buckles on line carries out signature analysis, and whether identification voltage signal undergos mutation；Recognizing the voltage signal
When undergoing mutation, whether the moment that binding mutation occurs, detection micropore there is plug-hole.
In preferred embodiment, the system also includes：
Plug-hole situation determining module 3, for judging moment that the mutation occurs whether in the range of the precognition mutation moment,
If it is not, judging that the micropore occurs plug-hole；
Specifically, precognition mutation moment scope was referred at the upper electric or lower electric moment, or some specific moment, and voltage is believed
Number can undergo mutation, voltage signal is undergone mutation in the range of these precognition mutation moment, is not belonging to the electricity that plug-hole causes
Pressure mutation, the voltage signal mutation occurred outside precognition mutation moment scope, then may be considered and to be caused by plug-hole.
Processing module 4, for the moment according to the micropore plug-hole, is reported to the police and/or follow-up unimpeded is processed.
In implementing, the moment that above-mentioned voltage signal is undergone mutation is recorded, if the moment is not mutated in above-mentioned precognition
In the range of moment, then represent micropore and plug-hole occurs, start alert event and alerted, the content of the warning message is mainly comprising micro-
There is plug-hole, the moment that further can also be undergone mutation comprising voltage signal in hole.Further, follow-up row can also be carried out
Stifled process, including carrying out cleaning etc. operation to micropore.
The micropore plug-hole detecting system provided using the present invention, its micropore voltage extraction circuit are loaded directly in detection micropore
The voltage change at micropore two ends on the positive and negative electrode of both sides, can be accurately detected, is adopted based on the micropore voltage extraction circuit
The voltage signal for collecting, can carry out signature analysis, the moment whether voltage signal is undergone mutation and be mutated be recognized, with accurate
Micropore plug-hole is effectively identified.
In implementing, the micropore voltage extraction circuit can with as shown in figure 3, the micropore voltage extraction circuit be connected to micro-
Between the positive and negative polarities of hole both sides, specifically：Connected between the positive and negative polarities of micropore by a micropore sensor, the micropore sensing
Device one end is connected with the base stage of triode Q1, and the other end is connected with the emitter stage of triode Q1 by resistance, and triode Q1 is used
In the voltage signal between the detection micropore the two poles of the earth is amplified, the emitter stage of triode Q1 is also connected with a signal conditioning circuit, its
For the micropore voltage signal that adjustment is amplified through triode Q1；Certainly the micropore voltage extraction circuit is merely illustrative, and which can be with
There are other equivalent ways of realization.
In addition, when being acquired to the voltage at micropore two ends using micropore voltage extraction circuit, can be with micro-
A resistance is added between the positive and negative electrode in hole, concrete as shown in Figure 4.Extract through the micropore voltage extraction circuit shown in Fig. 4
Micropore voltage signal.
In the voltage signal for collecting detection micropore two ends using micropore voltage extraction circuit, and record the voltage signal with
After forming micropore voltage curve, you can carry out signature analysis to the micropore voltage curve, accurately to judge micropore plug-hole.In tool
During body is realized, there are following several ways realize accurately judging micropore plug-hole.
First kind of way is to carry out differential process to the voltage signal on the micropore voltage curve to carry out plug-hole identification.
Carried out in plug-hole knowledge otherwise, as shown in figure 5, the micropore voltage by carrying out differential process to voltage signal
Characteristics analysis module is specifically included：
Differential process unit 20, for carrying out differential process to the voltage signal on the micropore voltage curve, obtains micro-
Divided voltage signal curve；Specifically, carrying out differential process to voltage signal also has multiple concrete implementation forms, for example：To micro-
The voltage signal that hole voltage extraction circuit is extracted carries out Digital Signal Processing, obtains differentiated voltage signal；Or by hardware electricity
Voltage signal is become differentiated voltage signal etc. by road.
First signal identification unit 21, for analyzing the differentiated voltage signal curve, for analyzing the differential voltage
Signal curve, recognizes whether the differentiated voltage signal exceedes the threshold value for setting, and thus judges the corresponding electricity of the differentiated voltage signal
Whether pressure signal undergos mutation.
It should be noted that carrying out differential process to the voltage signal on micropore voltage curve, practical function is to amplify electricity
The rate of change of pressure signal.When micropore does not occur plug-hole, voltage signal will not be undergone mutation, concrete such as Fig. 6, when
When micropore occurs plug-hole situation, as plug-hole is a process for mutation, therefore by carrying out differential process to voltage signal, so that it may
Whether to be undergone mutation according to voltage signal and the moment of binding mutation detects plug-hole.
If there is plug-hole to cell during counting through micropore, its original voltage curve and voltage are micro-
As shown in Figure 7 and Figure 8, if before counting there is plug-hole, its original voltage curve and voltage derivative signal in sub-signal curve
Curve is then as shown in Figure 9.The voltage signal for producing mutation, the voltage signal pair of the mutation can be significantly found out according to Fig. 7 ~ Fig. 9
The moment that answers is the moment that plug-hole occurs, and the amplitude of the voltage signal of the mutation can represent the degree of plug-hole.
The second way is that micropore voltage curve is carried out curve fitting to carry out plug-hole identification.
This mode is as shown in Figure 10, the micropore voltage characteristic analysis module, including：
Segment processing unit 22, for moving segment processing by the micropore voltage curve；
Curve matching unit 23, for the last period voltage curve according to the micropore voltage curve, fits thereafter one
Section voltage curve；
Secondary signal recognition unit 24, for the voltage curve and the micropore electricity that are fitted the curve matching unit 23
Line of buckling is analyzed, Recognition Different signal, and thus judges whether voltage signal undergos mutation.
Illustrate as a example by Figure 11 and Figure 12, normal micropore voltage curve should be as shown in figure 11, when being counted, its
Curve should smooth and level off to straight line, therefore can fitting according to the last period curve of the micropore voltage signal curve
One section of micropore voltage curve after going out under normal circumstances.Then again by actually detected for the such as Figure 12 micropore voltage signal curve for obtaining with
The micropore voltage curve 11 of fitting compares, you can identify the voltage signal for difference occur, and this electricity of difference occurs
Pressure signal represents that voltage signal is undergone mutation.
The third mode is that the micropore voltage curve by micropore voltage curve with standard compares, to carry out plug-hole
Identification.
This mode is as shown in figure 13, the micropore voltage characteristic analysis module, including：
Calibration curve signal generating unit 25, the voltage signal for micropore of the detection without plug-hole simultaneously form standard microwell voltage song
Line；
3rd signal identification unit 26, for the micropore voltage curve that generates the calibration curve signal generating unit 25 and institute
State standard microwell voltage curve to be analyzed, Recognition Different signal, and thus judge whether voltage signal undergos mutation.
In implementing, calibration curve signal generating unit 25 goes out a standard according to the normal micropore voltage statistic without plug-hole
Micropore voltage curve, as shown in figure 14, and then the micropore voltage curve is obtained detection such as Figure 15 as a template
Micropore voltage curve is mated or comparative analysis with the curve module, if mating, unsuccessful or correlation is relatively low, this goes out
The voltage signal of existing difference represents that voltage signal is undergone mutation.
Accordingly, the present invention also provides micropore plug-hole detection method, as shown in figure 16, including：
Step 100, the voltage signal between the positive and negative electrode of the detection micropore both sides for gathering Medical Devices, record collection institute
The multiple voltage signals for obtaining form micropore voltage curves；
Step 101, carries out signature analysis to the voltage signal on the micropore voltage curve, and whether identification voltage signal is sent out
Raw mutation.
Step 102, when recognizing the voltage signal and undergoing mutation, at the moment that binding mutation occurs, detection micropore is
No generation plug-hole.
Specifically, judge whether the moment that the mutation occurs is being predicted in the range of the mutation moment, if it is not, judging described micro-
There is plug-hole in hole.Precognition mutation moment scope was referred at the upper electric or lower electric moment, or some specific moment, and voltage signal is
Can undergo mutation, the voltage signal of the mutation occurred in the range of these precognition mutation moment is not belonging to the electricity that plug-hole causes
Pressure mutation, the voltage signal mutation occurred outside precognition mutation moment scope, then may be considered and to be caused by plug-hole.
Step 103, according to the moment of the micropore plug-hole, is reported to the police；
Step 104, according to the moment of the micropore plug-hole, carries out follow-up unimpeded process.
In implementing, the moment that above-mentioned voltage signal is undergone mutation is recorded, if the moment is not mutated in above-mentioned precognition
In the range of moment, then represent micropore and plug-hole occurs, start alert event and alerted, the content of the warning message is mainly comprising micro-
There is plug-hole, the moment that further can also be undergone mutation comprising voltage signal in hole.Further, follow-up row can also be carried out
Stifled process, including carrying out cleaning etc. operation to micropore.
In the voltage signal for collecting detection micropore two ends, and record the voltage signal with formed micropore voltage curve it
Afterwards, you can signature analysis is carried out to the micropore voltage curve, recognize the moment whether voltage signal is undergone mutation and be mutated, with
Accurately judge micropore plug-hole.In implementing, there are following several ways realize accurately judging micropore plug-hole.
First kind of way is to carry out differential process to the voltage signal on the micropore voltage curve to carry out plug-hole identification.
As shown in figure 17, first kind of way is specifically included：
Step 200, carries out differential process to the voltage signal on the micropore voltage curve, obtains differentiated voltage signal bent
Line；
Step 201, analyzes the differentiated voltage signal curve, recognizes whether the differentiated voltage signal exceedes the threshold for setting
Value, thus judges whether the corresponding voltage signal of the differentiated voltage signal undergos mutation.
It should be noted that carrying out differential process to the voltage signal on micropore voltage curve, practical function is to amplify electricity
The rate of change of pressure signal.When detecting that micropore does not occur plug-hole, voltage signal will not be undergone mutation, concrete as schemed
6, when detecting that micropore occurs plug-hole situation, as plug-hole is a process for mutation, therefore by carrying out differential to voltage signal
Process, it is possible to which the voltage signal according to mutation detects plug-hole moment and degree.
If there is plug-hole to cell during counting through micropore, its original voltage curve and voltage are micro-
As shown in Figure 7 and Figure 8, if before counting there is plug-hole, its original voltage curve and voltage derivative signal in sub-signal curve
Curve is then as shown in Figure 9.The voltage signal for producing mutation, the voltage signal pair of the mutation can be significantly found out according to Fig. 7 ~ Fig. 9
The moment that answers is the moment that plug-hole occurs, and the amplitude of the voltage signal of the mutation can represent the state or degree of plug-hole.
The second way is that micropore voltage curve is carried out curve fitting to carry out plug-hole identification.
As shown in figure 18, the second way includes：
The micropore voltage curve is moved segment processing by step 300；
Step 301, according to the last period voltage curve of the micropore voltage curve, fits one section of voltage curve thereafter；
Step 302, the voltage curve of fitting is analyzed with the micropore voltage curve, Recognition Different signal,
And thus judge whether voltage signal undergos mutation.
Illustrate as a example by Figure 11 and Figure 12, normal micropore voltage curve should be as shown in figure 14, when being counted, its
Curve should smooth and level off to straight line, therefore can fitting according to the last period curve of the micropore voltage signal curve
One section of micropore voltage curve after going out under normal circumstances.Then again by actually detected for the such as Figure 12 micropore voltage signal curve for obtaining with
The micropore voltage curve 11 of fitting compares, you can identify the voltage signal for difference occur, and this electricity of difference occurs
Pressure signal represents that voltage signal is undergone mutation.
The third mode is that the micropore voltage curve by micropore voltage curve with standard compares, to carry out plug-hole
Identification.
This mode as shown in figure 19, carries out signature analysis to the voltage signal on the micropore voltage curve, and identification is produced
The voltage signal of raw mutation, including：
Step 400, detects the voltage signal of the micropore without plug-hole and forms standard microwell voltage curve；
Step 401, the micropore voltage curve is analyzed with the standard microwell voltage curve, Recognition Different
Signal, and thus judge whether voltage signal undergos mutation.
In implementing, according to the micropore voltage curve that the normal micropore voltage statistic without plug-hole goes out a standard, such as scheme
Shown in 14, and using the micropore voltage curve as a template, then by detection obtain micropore voltage curve such as Figure 15 with should
Curve module is mated or comparative analysis, if the unsuccessful or correlation of coupling is relatively low, there is the voltage signal of difference in this
Represent that voltage signal is undergone mutation.
Implement the present invention, its advantage is as follows：
First, if using micropore voltage set in advance high-low threshold value come the method that carries out plug-hole judgement because its shadow
The factor of sound is more, such as the discreteness of micropore device, and the different machines between batch or even in batch can be caused to suffer from larger difference
Not, so the accurate setting of threshold value is an extremely difficult thing, it is easy to erroneous judgement occurs or fails to judge；And what the present invention was provided
Micropore plug-hole detecting system, its micropore voltage extraction circuit are carried on the positive and negative electrode of detection micropore both sides, accurate collection
Micropore voltage, then analyzes the variation characteristic of voltage signal, from the impact that these two aspects weakens extraneous factor, is exaggerated
The feature of plug-hole, so as on judging in plug-hole accurately and reliably.Change less obvious plug-hole situation in primary signal, using this
Which also can be identified by method exactly.
Second, used in prior art, micropore impedance method is counted, and needs to wait electricity on micropore just to open after a period of stabilisation
Begin to count, if so the method for passing through particle with pore number using the statistical unit time, the feelings that plug-hole occurs before starting counting
Shape accurately cannot be recognized；And the present invention has monitored the full range voltage on micropore after electricity, then do not limited by open-interval is counted
System, reduces the possibility that fails to judge.
3rd, the present invention while plug-hole phenomenon is accurately identified can also be accurately obtained its plug-hole and occur
Moment, can aid in completing the differentiation to plug-hole state, in order to the subsequent treatment of cellanalyzer.
Therefore, for the present invention is compared with conventional method, one is that accuracy of judgement is effective, is difficult to be affected by other factorses；Two be can
Start the front plug-hole phenomenon for just occurring to judge to count；Three is to recognize plug-hole temporal information, strengthens to plug-hole state
Judge.
One of ordinary skill in the art will appreciate that realize all or part of flow process in above-described embodiment method, being can be with
Instruct related hardware to complete by computer program, described program can be stored in a computer read/write memory medium
In, the program is upon execution, it may include such as the flow process of the embodiment of above-mentioned each method.Wherein, described storage medium can be magnetic
Dish, CD, read-only memory（Read-Only Memory, ROM）Or random access memory（Random Access
Memory, RAM）Deng.
Above content is further description made for the present invention with reference to specific preferred embodiment, it is impossible to assert
The present invention be embodied as be confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of without departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (9)
1. a kind of micropore plug-hole detection method, it is characterised in that include：
Voltage signal between the positive and negative electrode of the micropore both sides of collection Medical Devices, multiple voltage signals of record collection gained
Form micropore voltage curve；
Signature analysis is carried out to the voltage signal on the micropore voltage curve, and whether identification voltage signal undergos mutation；
When recognizing the voltage signal and undergoing mutation, whether the moment that binding mutation occurs, detection micropore there is plug-hole, wrap
Include：Judge whether the moment that the mutation occurs is being predicted in the range of the mutation moment, if it is not, judging that the micropore occurs plug-hole.
2. micropore plug-hole detection method as claimed in claim 1, it is characterised in that to the voltage on the micropore voltage curve
Signal carries out signature analysis, and whether identification voltage signal is undergone mutation, comprised the steps：
Differential process are carried out to the voltage signal on the micropore voltage curve, obtain differentiated voltage signal curve；
The differentiated voltage signal curve is analyzed, recognizes whether the differentiated voltage signal exceedes the threshold value for setting, thus judging should
Whether the corresponding voltage signal of differentiated voltage signal undergos mutation；
Or comprise the steps：
The micropore voltage curve is moved segment processing；
According to the last period voltage curve of the micropore voltage curve, one section of voltage curve thereafter is fitted；
The voltage curve of fitting is analyzed with the micropore voltage curve, Recognition Different signal, and thus judges electricity
Whether pressure signal undergos mutation；
Or comprise the steps：
The voltage signal of micropore of the detection without plug-hole, obtains standard microwell voltage curve；
The micropore voltage curve is analyzed with the standard microwell voltage curve, Recognition Different signal, and thus
Judge whether voltage signal undergos mutation.
3. micropore plug-hole detection method as claimed in claim 2, it is characterised in that recognize the voltage signal occur prominent
During change, at the moment that binding mutation occurs, after whether detection micropore occurs plug-hole, also include：
According to the moment of the micropore plug-hole, reported to the police and/or follow-up unimpeded is processed.
4. a kind of micropore plug-hole detecting system, it is characterised in that include：
Micropore voltage extraction circuit, its connect the positive and negative electrode of the micropore both sides of Medical Devices, for gathering between positive and negative electrode
Voltage signal, and record the multiple voltage signals of collection gained and form micropore voltage curves；
Micropore voltage characteristic analysis module, its are connected with the micropore voltage extraction circuit, for the micropore voltage curve
On voltage signal carry out signature analysis, whether identification voltage signal undergos mutation；Occur to dash forward the voltage signal is recognized
During change, whether the moment that binding mutation occurs, detection micropore there is plug-hole；
Wherein, the system also includes plug-hole determining module, for judging whether the moment that the mutation occurs is mutated in precognition
In the range of moment, if it is not, judging that the micropore occurs plug-hole.
5. micropore plug-hole detecting system as claimed in claim 4, it is characterised in that the micropore voltage characteristic analysis module,
Including：
Differential process unit, for carrying out differential process to the voltage signal on the micropore voltage curve, obtains differential voltage
Signal curve；
First signal identification unit, for analyzing the differentiated voltage signal curve, recognizes whether the differentiated voltage signal exceedes
The threshold value of setting, thus judges whether the corresponding voltage signal of the differentiated voltage signal undergos mutation.
6. micropore plug-hole detecting system as claimed in claim 4, it is characterised in that the micropore voltage characteristic analysis module,
Including：
Segment processing unit, for moving segment processing by the micropore voltage curve；
Curve matching unit, for the last period voltage curve according to the micropore voltage curve, fits one section of voltage thereafter
Curve；
Secondary signal recognition unit, the voltage curve and the micropore voltage curve for the curve matching unit to be fitted enter
Row comparative analysis, Recognition Different signal, and thus judge whether voltage signal undergos mutation.
7. micropore plug-hole detecting system as claimed in claim 4, it is characterised in that the micropore voltage characteristic analysis module,
Including：
Calibration curve signal generating unit, for the voltage signal of micropore of the detection without plug-hole, obtains standard microwell voltage curve；
3rd signal identification unit, is carried out to score for just described micropore voltage curve and the standard microwell voltage curve
Analysis, Recognition Different signal, and thus judge whether voltage signal undergos mutation.
8. the micropore plug-hole detecting system as described in any one of claim 5 to 7, it is characterised in that the system also includes：
Processing module, for the moment according to the micropore plug-hole, is reported to the police and/or follow-up unimpeded is processed.
9. a kind of blood cell analyzer, it is characterised in that include：Counting chamber, micropore and constant-current source, and such as claim 4
To a kind of micropore plug-hole detecting system any one of 8；Conductive solvent is full of in the counting chamber, and by the micropore point
It is segmented into forebay and after-bay；A positive electrode and a negative electrode, the positive and negative electrode and constant current is respectively arranged with forebay and after-bay
One end connection in source；The micropore plug-hole detecting system connects the positive electrode and negative electricity arranged in the forebay and after-bay respectively
Pole.
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| CN104535478B (en) * | 2015-01-07 | 2017-04-12 | 深圳市帝迈生物技术有限公司 | Hole blocking judging and result correcting method based on particle flow stability |
| CN111122841A (en) * | 2018-10-31 | 2020-05-08 | 深圳市帝迈生物技术有限公司 | Method for improving sample result accuracy and sample analyzer |
| CN111257204B (en) * | 2018-11-30 | 2021-11-23 | 深圳市帝迈生物技术有限公司 | Detection method for micropore blockage of sample analyzer and sample analyzer |
| CN111855977B (en) * | 2019-04-30 | 2021-12-14 | 深圳市帝迈生物技术有限公司 | Blood analysis system, cleansing liquid calculation device, and cleansing liquid calculation method |
| CN112557282B (en) * | 2020-11-17 | 2023-06-16 | 深圳市科曼医疗设备有限公司 | Small hole blocking recognition method and device for blood cell analyzer |
| CN112630109A (en) * | 2020-12-01 | 2021-04-09 | 广微(中山)智能科技有限公司 | Real-time online detector for particle size of microparticles in high-concentration weak conductive liquid and detection method thereof |
| CN112730202B (en) * | 2020-12-29 | 2023-06-16 | 深圳市科曼医疗设备有限公司 | Impedance method detection system of blood cell analyzer and method for identifying impedance channel blocking hole |
| CN114441415B (en) * | 2022-04-06 | 2022-09-20 | 深圳市帝迈生物技术有限公司 | Micro-plugging hole identification method and sample analyzer |
| CN114441414B (en) * | 2022-04-06 | 2022-09-20 | 深圳市帝迈生物技术有限公司 | Hole blockage judging method and sample analyzer |
| CN116481982B (en) * | 2023-04-20 | 2024-09-20 | 瑞芯智造(深圳)科技有限公司 | Particle detection method based on Coulter particle size detector and detector |
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