CN112730202B - Impedance method detection system of blood cell analyzer and method for identifying impedance channel blocking hole - Google Patents

Impedance method detection system of blood cell analyzer and method for identifying impedance channel blocking hole Download PDF

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CN112730202B
CN112730202B CN202011592483.0A CN202011592483A CN112730202B CN 112730202 B CN112730202 B CN 112730202B CN 202011592483 A CN202011592483 A CN 202011592483A CN 112730202 B CN112730202 B CN 112730202B
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CN112730202A (en
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王兴红
邹海涛
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Shenzhen Comen Medical Instruments Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects thereof, e.g. conductivity or capacity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/12—Coulter-counters
    • G01N15/01—
    • G01N2015/012—
    • G01N2015/1026—
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The application discloses an impedance method detecting system of a blood cell analyzer and a method for identifying an impedance channel blocking hole, wherein the method for identifying the impedance channel blocking hole comprises the following steps: and acquiring historical average pulse data, and solving the average value and standard deviation of the historical average pulse data. And acquiring the data of the voltage pulse analyzed by the sampling, and counting the average pulse of the cells passing through the small holes in each unit time. And sequentially calculating a front average value and a rear average value of each unit time, and judging the unit time which is likely to block the hole according to the change condition of the front average value and the rear average value of each unit time. And judging whether the hole is blocked or not in the unit time of possible hole blocking for the first time, comparing the second variation with the standard deviation of the historical average pulse data after judging that the hole is not blocked in the unit time of possible hole blocking for the first time, and judging whether the hole is blocked or not in the unit time of possible hole blocking for the second time. The recognition and reporting of the whole-process hole blocking condition are realized.

Description

Impedance method detection system of blood cell analyzer and method for identifying impedance channel blocking hole
Technical Field
The application relates to the technical field of particle detection, in particular to an impedance method detection system of a blood cell analyzer and a method for identifying a blocked hole of an impedance channel.
Background
When particles (cells) are detected by an impedance method, the small holes are immersed in an electrolyte solution, constant current power supplies are connected to the two ends of the small holes, and when the particles (cells) pass through the small holes, the voltage at the two ends of the small holes changes, and the larger the volume of the particles (cells), the larger the voltage change (the larger the voltage pulse). Taking the application of the blood detection field as an example, according to this principle, blood is diluted and mixed uniformly in a diluent (a reagent having conductivity and having the characteristic of physiological saline), and then a pulse (volume) of blood cells passing through the small holes can be obtained by having the small holes at both ends.
When detecting particles (cells) by using the impedance method, a hole blocking phenomenon sometimes occurs, that is, the particles (cells) to be detected cannot pass through the holes due to the blocking of the holes, which affects the continuation of the detection. After dilution and mixing, the blood cells are uniformly distributed in the solution, so that the average pulse size of all blood cells passing through the pores per unit time (e.g., 100 ms) should be uniform. If a hole is blocked at a certain time, the average pulse of cells before and after the certain time is very different. Thus, some methods determine whether a hole is plugged by a change in the curve of small Kong Dianya, and consider that a hole is plugged if the voltage of the hole changes significantly after a certain time. However, when the hole is plugged in the whole process, the voltage of the small hole may not change obviously, and the method cannot accurately report the hole plugging.
Disclosure of Invention
The invention mainly solves the technical problems that: the existing method can not accurately report the hole blocking during the whole hole blocking process.
In a first aspect, an embodiment provides an impedance method detection system for a blood cell analyzer, comprising:
a detection chamber including a small hole; the detection chamber is used for receiving a blood sample, a hemolysis agent and a diluent to prepare a sample to be measured;
a pressure source means for providing pressure to cause cells of the sample in the detection chamber to pass through the aperture;
the two ends of the constant current power supply are respectively and electrically connected with the two ends of the small hole;
the voltage pulse detection device is connected with the constant current power supply and is used for detecting voltage pulses generated when cells pass through the small holes and sending data of the voltage pulses;
the memory is used for receiving and storing historical average pulse data and the data of the voltage pulses sent by the voltage power supply pulse detection device; and
the processor is used for acquiring historical average pulse data and solving an average value and a standard deviation of the historical average pulse data; acquiring the data of the voltage pulse analyzed by the sampling, and counting the average pulse of the cells passing through the small holes in each unit time; defining the average value of the average pulse in all unit time before each unit time as a front average value, sequentially calculating the front average value of each unit time, defining the average value of the average pulse in all unit time after each unit time as a rear average value, sequentially calculating the rear average value of each unit time, and judging the unit time which is likely to be blocked according to the change conditions of the front average value and the rear average value of each unit time; calculating a first variation between a front average value of the unit time of the possible hole blockage and a rear average value of the unit time of the possible hole blockage, comparing the first variation with standard deviations of average pulses of all unit time before the unit time of the possible hole blockage and/or after the unit time of the possible hole blockage, judging whether the unit time of the possible hole blockage occurs for the first time, if the unit time of the possible hole blockage occurs for the first time, reporting abnormality, and if the unit time of the possible hole blockage does not occur for the first time, continuing to execute the next step; after judging that the hole is likely to be blocked in the unit time, calculating the average value of the average pulse in all unit time of the current sampling, calculating a second variation between the average value of the average pulse in all unit time of the current sampling and the average value of the historical average pulse data, comparing the second variation with the standard deviation of the historical average pulse data, judging whether the hole is likely to be blocked in the unit time of the hole for the second time, reporting abnormality if the hole is likely to be blocked in the second time, and sending the average value of the average pulse in all unit time of the current sampling to a memory and storing the historical average pulse data if the hole is not blocked in the second time.
In one embodiment, the processor obtains historical average pulse data, averages and standard deviations the historical average pulse data, comprising:
acquiring a linked list PreMPList of historical average pulse data, wherein the historical average pulse data is the average value of average pulses of all time units sampled in the past, acquiring a calculation formula of the average value of the historical average pulse data, calculating the average value MPre of the historical average pulse data, acquiring a calculation formula of standard deviation of the historical average pulse data, and calculating the standard deviation SPre of the historical average pulse data;
the calculation formula of the average value of the historical average pulse data comprises the following steps:
Figure BDA0002869043550000021
wherein n is the number of data in PreMPList;
the calculation formula of the standard deviation of the historical average pulse data comprises the following steps:
Figure BDA0002869043550000022
wherein n is the number of data in PreMPList.
In one embodiment, the processor obtains the data of the voltage pulse analyzed by the sampling, counts the average pulse of the cells passing through the small hole in each unit time, and includes:
acquiring total acquisition time T, acquiring unit time delta T, acquiring a calculation formula of total number N of unit time, and calculating total number N of unit time, wherein the calculation formula of total number N of unit time comprises:
N＝T/Δt，
Acquiring a formula for calculating the number of pulses in the ith unit time, and calculating the number of pulses in the ith unit time, wherein the formula for calculating the number of pulses in the ith unit time comprises the following steps:
m i ＝count(p(t,v))，
wherein, the value range of t is (i-1) delta t is less than or equal to t and less than i delta t, the value range of i is more than 0 and less than or equal to N, and i is a positive integer;
obtaining a calculation formula for calculating the average pulse of the ith unit time, and calculating the average pulse of the ith unit time, wherein the calculation formula for calculating the average pulse of the ith unit time comprises the following steps:
P i ＝(∑v)/m i ，
wherein v is p (t, v), the value range of t is (i-1) delta t is less than or equal to t and less than i delta t, the value range of i is more than 0 and less than or equal to N, and i is a positive integer;
the average pulse in each unit time is sequentially stored in the memory array P to obtain a memory array P= [ P ] 1 ,P 2 ,…,P i ,…,P N ]。
In one embodiment, the processor calculates a front average value of each unit time in turn, and calculates a rear average value of each unit time in turn, including:
acquiring a memory group P, acquiring a calculation formula for calculating a front average value of each unit, and sequentially calculating a front average value S1 of each unit time i The calculation formula of the front average value of each unit comprises：
Figure BDA0002869043550000031
Wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring a calculation formula for calculating the back average value of each unit, and sequentially calculating the back average value S2 of each unit time i The calculation formula of the post-mean value of each unit comprises the following steps:
Figure BDA0002869043550000032
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring initialized linked List1 and linked List2, and obtaining the front average value S1 of each unit time i Is stored in a List1 to obtain a linked List1[ i ]]The post-average value S2 of each unit time i Is stored in a List2 to obtain a linked List2[ i ]]。
In one embodiment, the processor determines the unit time of possible hole blocking according to the variation of the front average value and the rear average value of each unit time, including:
acquiring a List1[ i ] and a List2[ i ], acquiring a differential calculation formula, carrying out differential calculation on data in the List1[ i ], acquiring an initialized List DList1, storing the differential calculation result of the List1[ i ] into the List DList1 to obtain the List DList1[ i ], carrying out differential calculation on the data in the List2[ i ], acquiring an initialized List DList2, storing the differential calculation result of the List2[ i ] into the List DList2 to obtain the List DList2[ i ], wherein the differential calculation formula comprises:
DList1[i]＝List1[i+1]-List1[i]，
DList2[i]＝List2[i+1]-List2[i]，
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring a unit time judging formula of a possible hole blockage, wherein the unit time judging formula of the possible hole blockage comprises the following steps:
Dlist2[i]*Dlist1[i+1]<0，
Traversing the linked list DList2[ i ], inputting the data in the linked list DList1[ i ] and the linked list DList2[ i ] into the unit time judgment formula of the possible hole blocking, and if the data accords with the judgment formula of the possible hole blocking, the (i+1) th unit time is the unit time of the possible hole blocking.
In one embodiment, the processor determines the unit time of possible hole blocking according to the variation of the front average value and the rear average value of each unit time, including:
acquiring a List1[ i ] and a List2[ i ], acquiring a differential calculation formula, carrying out differential calculation on data in the List1[ i ], acquiring an initialized List DList1, storing the differential calculation result of the List1[ i ] into the List DList1 to obtain the List DList1[ i ], carrying out differential calculation on the data in the List2[ i ], acquiring an initialized List DList2, storing the differential calculation result of the List2[ i ] into the List DList2 to obtain the List DList2[ i ], wherein the differential calculation formula comprises:
DList1[i]＝List1[i+1]-List1[i]，
DList2[i]＝List2[i+1]-List2[i]，
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring a unit time judging formula of a possible hole blockage, wherein the unit time judging formula of the possible hole blockage comprises the following steps:
Dlist1[i]*Dlist2[i+1]<0，
traversing the linked list DList1[ i ], inputting the data in the linked list DList1[ i ] and the linked list DList2[ i ] into the unit time judgment formula of the possible hole blocking, and if the data accords with the judgment formula of the possible hole blocking, the (i+1) th unit time is the unit time of the possible hole blocking.
In one embodiment, the processor determines for the first time whether a possible hole blockage has occurred per unit time, including:
acquiring a record group P, acquiring a calculation formula of the average value of the average pulse of the first i unit time, wherein the i+1th unit time is the unit time which can block holes, and calculating the average value MP1 of the average pulse of the first i unit time, wherein the calculation formula of the average value of the average pulse of the first i unit time comprises:
Figure BDA0002869043550000051
wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a calculation formula of standard deviation of average pulse of the first i unit time, and calculating the standard deviation SP1 of the average pulse of the first i unit time, wherein the calculation formula of the standard deviation of the average pulse of the first i unit time comprises:
Figure BDA0002869043550000052
wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a calculation formula of an average value of the average pulses in the (i+1) th to N unit time, and calculating an average value MP2 of the average pulses in the (i+1) th to N unit time, wherein the calculation formula of the average value of the average pulses in the (i+1) th to N unit time comprises:
Figure BDA0002869043550000053
wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a calculation formula of standard deviation of average pulses in the (i+1) th to N unit time, and calculating the standard deviation SP2 of the average pulses in the (i+1) th to N unit time, wherein the calculation formula of the standard deviation of the average pulses in the (i+1) th to N unit time comprises:
Figure BDA0002869043550000054
Wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a judging formula of first judgment, wherein the judging formula of the first judgment comprises:
|MP1-MP2|＜SP1，
|MP1-MP2|＜SP2，
and inputting the average value MP1 of the average pulse in the first i unit time, the standard deviation SP1 of the average pulse in the first i unit time, the average value MP2 of the average pulse in the i+1 to N unit time and the standard deviation SP2 of the average pulse in the i+1 to N unit time into a judging formula for the first judgment, wherein if at least one formula in the judging formula for the first judgment is established, the first judgment is that the hole is blocked, otherwise, the first judgment is that the hole is not blocked.
In one embodiment, the processor determines, for a second time, whether a possible hole blockage has occurred per unit time, including:
acquiring a memory group P, acquiring a calculation formula of the average value of the average pulse in all unit time of the current sample, and calculating the average value MP of the average pulse in all unit time of the current sample, wherein the calculation formula of the average value of the average pulse in all unit time of the current sample comprises:
Figure BDA0002869043550000061
acquiring a mean value MPre of the historical average pulse data and a standard deviation SPre of the historical average pulse data, acquiring a judgment formula of the second judgment, substituting MP, MPre and SPre into the judgment formula of the second judgment,
If the formula
|MPre-MP|＜3SPre
If so, judging that the hole blockage does not occur for the second time;
if the formula
|MPre-MP|≥3SPre
If so, judging that the whole process hole blocking occurs for the second time.
In a second aspect, in one embodiment, a method for identifying a plugged hole in an impedance channel is provided, for detecting whether a plugged hole has occurred in an impedance detection system of a blood cell analyzer, including:
acquiring historical average pulse data, and solving an average value and a standard deviation of the historical average pulse data;
acquiring the data of the voltage pulse analyzed by the sampling, and counting the average pulse of the cells passing through the small holes in each unit time;
defining the average value of the average pulse in all unit time before each unit time as a front average value, sequentially calculating the front average value of each unit time, defining the average value of the average pulse in all unit time after each unit time as a rear average value, sequentially calculating the rear average value of each unit time, and judging the unit time which is likely to be blocked according to the change conditions of the front average value and the rear average value of each unit time;
calculating a first variation between a front average value of the unit time of the possible hole blockage and a rear average value of the unit time of the possible hole blockage, comparing the first variation with standard deviations of average pulses of all unit time before the unit time of the possible hole blockage and/or after the unit time of the possible hole blockage, judging whether the unit time of the possible hole blockage occurs for the first time, if the unit time of the possible hole blockage occurs for the first time, reporting abnormality, and if the unit time of the possible hole blockage does not occur for the first time, continuing to execute the next step;
After judging that the blocking of the holes is possible in the unit time for the first time, calculating the average value of the average pulse of all unit time of the current sampling, calculating a second variation between the average value of the average pulse of all unit time of the current sampling and the average value of the historical average pulse data, comparing the second variation with the standard deviation of the historical average pulse data, judging whether the blocking of the holes is possible in the unit time for the second time, if the blocking of the holes is possible in the second time, reporting the abnormality, and if the blocking of the holes is not possible in the second time, storing the average value of the average pulse of all unit time of the current sampling into the historical average pulse data.
In a third aspect, an embodiment provides a computer readable storage medium having a program stored thereon, the program being executable by a processor to implement the method described above.
According to the impedance method detection system and the impedance channel plugging detection method of the blood cell analyzer, the second variation is compared with the standard deviation of the historical average pulse data, so that whether the plugging possibly occurs in unit time of the plugging is judged for the second time, and the whole-process plugging condition is identified and reported.
Drawings
FIG. 1 is a schematic diagram of the Coulter principle;
FIG. 2 is a schematic diagram of impedance method detection;
FIG. 3 is a schematic diagram of an impedance detection system of a blood cell analyzer according to an embodiment;
FIG. 4 is a flow chart of a method for identifying impedance channel plugging in one embodiment;
FIG. 5 is a graphical representation of the data in List2[ i ] with no hole plugging in one embodiment;
FIG. 6 is a graphical representation of the data in List2[ i ] for plugging a hole in one embodiment.
Detailed Description
The invention will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present application. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, some operations associated with the present application have not been shown or described in the specification to avoid obscuring the core portions of the present application, and may not be necessary for a person skilled in the art to describe in detail the relevant operations based on the description herein and the general knowledge of one skilled in the art.
Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.
The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The terms "coupled" and "connected," as used herein, are intended to encompass both direct and indirect coupling (coupling), unless otherwise indicated.
Referring to fig. 1 and 2, fig. 1 is a schematic diagram of the coulter principle, in which a small hole is immersed in an electrolyte solution, a constant current power supply is connected to two ends of the small hole, and when a tiny particle passes through the small hole, the voltage at two ends of the small hole changes, and the larger the volume of the tiny particle, the larger the change of the voltage (the larger the voltage pulse).
Fig. 2 is a schematic diagram of impedance method (coulter principle) detection, in which blood is diluted and mixed uniformly in a diluent (a reagent having conductivity and physiological saline characteristics) according to the coulter principle, and then a pulse (volume) passing through small-hole cells can be obtained by having small holes at both ends.
After dilution and mixing of the blood cells, they are uniformly distributed in the solution, so that the average pulse size per unit time (e.g., 100 ms) of all cells passing through the pores should be uniform. If a hole is blocked at a certain time, the average pulse of cells before and after the certain time is very different. The method is based on the principle to realize the judgment of hole blocking.
The instrument records not only the size of the pulse but also the time it takes to record the pulse, i.e. each pulse has a two dimensional sign, i.e. the size v of the pulse value, respectively, and the time t at which the pulse is generated, p (t, v) representing a pulse. T is the total duration of the sample acquisition (in s).
In one aspect, the present embodiments provide an impedance method detection system for a blood cell analyzer.
Referring to fig. 3, the impedance method detection system includes a detection chamber 1, a pressure source 2, a constant current power supply 3, a voltage pulse detection device 4, a memory 5 and a processor 6.
The detection chamber 1 comprises an aperture, and the detection chamber 1 is adapted to receive a blood sample, a hemolytic agent and a diluent for preparing a sample to be assayed. The pressure source part 2 is used for providing pressure to enable cells of the sample in the detection chamber 1 to pass through the small hole, and two ends of the constant current power supply 3 are respectively and electrically connected with two ends of the small hole. The voltage pulse detection device 4 is connected with the constant current power supply 3 and is used for detecting voltage pulses generated when cells pass through the small holes and sending data of the voltage pulses. The memory 5 is used for receiving and storing historical average pulse data and data of the voltage pulses transmitted by the voltage supply pulse detection means 4.
The processor 6 is configured to obtain historical average pulse data, and average and standard deviation of the historical average pulse data. And acquiring the data of the voltage pulse analyzed by the sampling, and counting the average pulse of the cells passing through the small holes in each unit time. The average value of the average pulse in each unit time before each unit time is defined as a front average value, the front average value of each unit time is calculated in sequence, the average value of the average pulse in each unit time after each unit time is defined as a rear average value, the rear average value of each unit time is calculated in sequence, and the unit time which is likely to be blocked is judged according to the change conditions of the front average value and the rear average value of each unit time. Calculating a first variation between a front average value of the unit time of the possible hole blockage and a rear average value of the unit time of the possible hole blockage, comparing the first variation with standard deviations of average pulses of all unit time before the unit time of the possible hole blockage and/or after the unit time of the possible hole blockage, judging whether the unit time of the possible hole blockage occurs for the first time, if the unit time of the possible hole blockage occurs for the first time, reporting abnormality, and if the unit time of the possible hole blockage does not occur for the first time, continuing to execute the next step. After judging that the blocking of the holes is possible in the unit time for the first time, calculating the average value of the average pulse in all unit time of the current sampling, calculating a second variation between the average value of the average pulse in all unit time of the current sampling and the average value of the historical average pulse data, comparing the second variation with the standard deviation of the historical average pulse data, judging whether the blocking of the holes is possible in the unit time for the second time, if the blocking of the holes is possible in the second time, reporting abnormality, and if the blocking of the holes is not possible in the second time, sending the average value of the average pulse in all unit time of the current sampling to a memory, and storing the historical average pulse data.
In another aspect, the present embodiment provides a method for identifying a plugged hole in an impedance channel, where the method is used for detecting whether a plugged hole occurs in an impedance method detection system of a blood cell analyzer.
The method for identifying the blocking hole of the impedance channel has the following advantages:
first, some schemes determine whether the hole is blocked by the stability of the particle flow, and if the particle flow jumps after a certain time, the hole is considered to be blocked. However, when the hole is blocked in the whole process, the particle flow is not obviously changed, and the accurate hole blocking cannot be accurately reported. When the sample is insufficiently mixed, the particle flow stability cannot be ensured, and the hole is easily blocked by false alarm in the scheme. Compared with the scheme, the method of the embodiment is added with the comparison of the second variation and the standard deviation of the historical average pulse data, so that whether the hole is blocked or not in unit time which is possibly blocked is judged for the second time, and the recognition and the report of the whole-process hole blocking condition are realized. The problem that the particle flow jump misinformation is used for blocking holes due to poor mixing is solved.
Second, some schemes determine whether to block the hole by changing the curve of small Kong Dianya, however, when the voltage of the whole-process Kong Shixiao hole is blocked, the voltage may not change obviously, and the hole blocking cannot be accurately reported. Compared with the scheme, the method of the embodiment realizes the identification and reporting of the whole-process hole blocking condition.
Thirdly, some schemes take the pore voltage at the starting time as a reference, and combine the change of a small Kong Dianya curve to judge whether the pore is blocked, if the pore voltage is unchanged but obviously higher than the starting voltage, the whole process pore blocking is judged, and if the pore voltage has obvious change at a certain moment, the pore blocking is considered to occur. However, when the temperature of the diluent and the temperature at the time of starting up have obvious differences, the reference voltage at the time of starting up is invalid due to the fact that the conductivities of the diluent are different at different temperatures, and the hole blockage cannot be accurately reported. The method of the present embodiment is less susceptible to temperature variation than the above-described approach.
Referring to fig. 4, the method for identifying the blocking hole of the impedance channel includes:
referring to fig. 4, in step 100, historical average pulse data is obtained, and the average value and standard deviation of the historical average pulse data are calculated.
In one embodiment, acquiring historical average pulse data, averaging and standard deviation of the historical average pulse data, comprises:
acquiring a linked list PreMPList of historical average pulse data, wherein the historical average pulse data is the average value of average pulses of all time units sampled in the past, acquiring a calculation formula of the average value of the historical average pulse data, calculating the average value MPre of the historical average pulse data, acquiring a calculation formula of the standard deviation of the historical average pulse data, and calculating the standard deviation SPre of the historical average pulse data.
The calculation formula of the average value of the historical average pulse data comprises:
Figure BDA0002869043550000101
wherein n is the number of data in PreMPList.
The calculation formula of the standard deviation of the historical average pulse data comprises:
Figure BDA0002869043550000102
wherein n is the number of data in PreMPList.
Referring to fig. 4, step 200 obtains the data of the voltage pulse analyzed by the current sampling, and counts the average pulse of the cells passing through the small holes in each unit time.
In one embodiment, obtaining data of the voltage pulse analyzed by the sampling, counting average pulse of cells passing through the pores in each unit time, includes:
the method comprises the steps of obtaining total acquisition time T, obtaining unit time delta T, obtaining a calculation formula of total number N of unit time, and calculating the total number N of unit time, wherein the calculation formula of the total number N of unit time comprises:
N＝T/Δt，
specifically, Δt in this embodiment takes 0.1s, and in other embodiments, Δt may also select other suitable values according to actual requirements.
Acquiring a formula for calculating the number of pulses in the ith unit time, and calculating the formula for calculating the number of pulses in the ith unit time comprises the following steps:
m i ＝count(p(t,v))，
wherein, the value range of t is (i-1) delta t is less than or equal to t and less than i delta t, the value range of i is more than 0 and less than or equal to N, and i is a positive integer.
Obtaining a calculation formula for calculating the average pulse of the ith unit time, and calculating the calculation formula for the average pulse of the ith unit time, wherein the calculation formula comprises the following steps:
P i ＝(∑v)/m i ，
wherein v is p (t, v), the value range of t is (i-1) delta t is less than or equal to t and less than i delta t, the value range of i is more than 0 and less than or equal to N, and i is a positive integer.
The average pulse in each unit time is sequentially stored in the memory array P to obtain a memory array (vector) P= [ P ] 1 ,P 2 ,…,P i ,…,P N ]。
Referring to fig. 4, step 300 defines the average value of the average pulse in each unit time before each unit time as a front average value, sequentially calculates the front average value of each unit time, defines the average value of the average pulse in each unit time after each unit time as a rear average value, and sequentially calculates the rear average value of each unit time.
In one embodiment, sequentially calculating the front average value for each unit time and sequentially calculating the rear average value for each unit time includes:
acquiring a memory group P, acquiring a calculation formula for calculating a front average value of each unit, and sequentially calculating each unit timeFront average S1 of (1) i The calculation formula of the front average value of each unit includes:
Figure BDA0002869043550000111
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer.
Acquiring a calculation formula for calculating the back average value of each unit, and sequentially calculating the back average value S2 of each unit time i The calculation formula of the post-mean value of each unit includes:
Figure BDA0002869043550000112
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer.
Acquiring initialized linked List1 and linked List2, and obtaining the front average value S1 of each unit time i Is stored in a List1 to obtain a linked List1[ i ]]Please refer to fig. 5 and 6, the post-mean value S2 of each unit time i Is stored in a List2 to obtain a linked List2[ i ]]。
Referring to fig. 4, in step 400, a unit time of possible hole blocking is determined according to the variation of the front average value and the rear average value of each unit time.
In one embodiment, the determining the unit time of the possible hole blockage according to the change condition of the front average value and the rear average value of each unit time includes:
acquiring a List1[ i ] and a List2[ i ], acquiring a differential calculation formula, carrying out differential calculation on data in the List1[ i ], acquiring an initialized List DList1, storing the differential calculation result of the List1[ i ] into the List DList1 to obtain the List DList1[ i ], carrying out differential calculation on the data in the List2[ i ], acquiring an initialized List DList2, storing the differential calculation result of the List2[ i ] into the List DList2 to obtain the List DList2[ i ], wherein the differential calculation formula comprises:
DList1[i]＝List1[i+1]-List1[i]，
DList2[i]＝List2[i+1]-List2[i]，
Wherein, i is more than 0 and less than or equal to N-1, i is a positive integer.
Obtaining a unit time judging formula of the possible hole blockage, wherein the unit time judging formula of the possible hole blockage comprises the following steps:
Dlist2[i]*Dlist1[i+1]<0，
traversing the linked list DList2[ i ], inputting the data in the linked list DList1[ i ] and the linked list DList2[ i ] into a unit time judging formula of possible hole blocking, and if the data accords with the judging formula of possible hole blocking, the (i+1) th unit time is the unit time of possible hole blocking.
In another embodiment, the judging the unit time of the possible hole blockage according to the change condition of the front average value and the rear average value of each unit time includes:
acquiring a List1[ i ] and a List2[ i ], acquiring a differential calculation formula, carrying out differential calculation on data in the List1[ i ], acquiring an initialized List DList1, storing the differential calculation result of the List1[ i ] into the List DList1 to obtain the List DList1[ i ], carrying out differential calculation on the data in the List2[ i ], acquiring an initialized List DList2, storing the differential calculation result of the List2[ i ] into the List DList2 to obtain the List DList2[ i ], wherein the differential calculation formula comprises:
DList1[i]＝List1[i+1]-List1[i]，
DList2[i]＝List2[i+1]-List2[i]，
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer.
Obtaining a unit time judging formula of the possible hole blockage, wherein the unit time judging formula of the possible hole blockage comprises the following steps:
Dlist1[i]*Dlist2[i+1]<0，
Traversing the linked list DList1[ i ], inputting the data in the linked list DList1[ i ] and the linked list DList2[ i ] into a unit time judging formula of possible hole blocking, and if the data accords with the judging formula of possible hole blocking, the (i+1) th unit time is the unit time of possible hole blocking.
Referring to fig. 4, in step 500, a first variation between a front average value of a unit time of a possible hole blockage and a rear average value of a unit time of the possible hole blockage is calculated, the first variation is compared with a standard deviation of average pulses of all unit time before the unit time of the possible hole blockage and/or after the unit time of the possible hole blockage, whether the unit time of the possible hole blockage occurs is judged for the first time, if the first time judges that the hole blockage occurs, an abnormality is reported, and if the first time judges that the hole blockage does not occur, the next step is continuously executed.
In one embodiment, the first determination of whether a possible hole blockage occurred per unit time includes:
the method comprises the steps of obtaining a record group P, obtaining a calculation formula of an average value of average pulses of the first i unit time, wherein the i+1th unit time is the unit time which can block holes, and calculating the average value MP1 of the average pulses of the first i unit time, wherein the calculation formula of the average value of the average pulses of the first i unit time comprises the following steps:
Figure BDA0002869043550000121
Wherein, the value range of i is more than 0 and less than N, and i is a positive integer.
Acquiring a calculation formula of standard deviation of the average pulse of the first i unit time, and calculating the standard deviation SP1 of the average pulse of the first i unit time, wherein the calculation formula of the standard deviation of the average pulse of the first i unit time comprises:
Figure BDA0002869043550000122
wherein, the value range of i is more than 0 and less than N, and i is a positive integer.
Acquiring a calculation formula of an average value of the average pulses in the (i+1) th to N unit time, and calculating an average value MP2 of the average pulses in the (i+1) th to N unit time, wherein the calculation formula of the average value of the average pulses in the (i+1) th to N unit time comprises:
Figure BDA0002869043550000131
wherein, the value range of i is more than 0 and less than N, and i is a positive integer.
Acquiring a calculation formula of standard deviation of average pulses in the (i+1) th to N unit time, and calculating the standard deviation SP2 of the average pulses in the (i+1) th to N unit time, wherein the calculation formula of the standard deviation of the average pulses in the (i+1) th to N unit time comprises:
Figure BDA0002869043550000132
wherein, the value range of i is more than 0 and less than N, and i is a positive integer.
Acquiring a judging formula of the first judgment, wherein the judging formula of the first judgment comprises:
|MP1-MP2|＜SP1，
|MP1-MP2|＜SP2，
the average value MP1 of the average pulse in the first i unit time, the standard deviation SP1 of the average pulse in the first i unit time, the average value MP2 of the average pulse in the i+1 to N unit time and the standard deviation SP2 of the average pulse in the i+1 to N unit time are input into a judging formula for the first judgment, if at least one formula in the judging formula for the first judgment is established, the hole blocking is judged to occur for the first time, otherwise, the hole blocking is not judged to occur for the first time. And after judging that the hole is blocked, reporting abnormal white/red cell channels, and issuing a blocking removal instruction. The "first variation" is |mp1-mp2| in the formula.
Referring to fig. 4, step 600, after determining that the hole blockage is likely to occur in the unit time for the first time, calculating the average value of the average pulse in all the unit time of the current sample, calculating a second variation between the average value of the average pulse in all the unit time of the current sample and the average value of the historical average pulse data, comparing the second variation with the standard deviation of the historical average pulse data, determining whether the hole blockage is likely to occur in the unit time of the hole blockage for the second time, if the hole blockage is likely to occur in the second time, reporting an abnormality, and if the hole blockage is not likely to occur in the second time, storing the average value of the average pulse in all the unit time of the current sample into the historical average pulse data.
In one embodiment, the second determining whether a possible hole blockage occurred per unit time includes:
the method comprises the steps of obtaining a memory group P, obtaining a calculation formula of the average value of average pulses in all unit time of the current sample, and calculating the average value MP of the average pulses in all unit time of the current sample, wherein the calculation formula of the average value of the average pulses in all unit time of the current sample comprises the following steps:
Figure BDA0002869043550000133
acquiring a mean value MPre of the historical average pulse data and a standard deviation SPre of the historical average pulse data, acquiring a judgment formula of the second judgment, substituting MP, MPre and SPre into the judgment formula of the second judgment,
If the formula
|MPre-MP|＜3SPre
If so, the second time that the hole blockage does not occur is judged.
If the formula
|MPre-MP|≥3SPre
If so, judging that the whole process hole blocking occurs for the second time. And after judging that the hole is blocked, reporting abnormal white/red cell channels, and issuing a blocking removal instruction. The "second variation" is |mpre-mp| in the formula.
In another aspect, the present embodiment provides a computer-readable storage medium having a program stored thereon, the program being executable by a processor to implement the above-described method.
The foregoing description of the invention has been presented for purposes of illustration and description, and is not intended to be limiting. Several simple deductions, modifications or substitutions may also be made by a person skilled in the art to which the invention pertains, based on the idea of the invention.

Claims (10)

1. An impedance method detection system for a blood cell analyzer, comprising:
a detection chamber including a small hole; the detection chamber is used for receiving a blood sample, a hemolysis agent and a diluent to prepare a sample to be measured;
a pressure source means for providing pressure to cause cells of the sample in the detection chamber to pass through the aperture;
the two ends of the constant current power supply are respectively and electrically connected with the two ends of the small hole;
The voltage pulse detection device is connected with the constant current power supply and is used for detecting voltage pulses generated when cells pass through the small holes and sending data of the voltage pulses;
a memory for receiving and storing historical average pulse data and data of the voltage pulses transmitted by the voltage pulse detecting device; and
the processor is used for acquiring historical average pulse data and solving an average value and a standard deviation of the historical average pulse data; acquiring the data of the voltage pulse analyzed by the sampling, and counting the average pulse of the cells passing through the small holes in each unit time; defining the average value of the average pulse in all unit time before each unit time as a front average value, sequentially calculating the front average value of each unit time, defining the average value of the average pulse in all unit time after each unit time as a rear average value, sequentially calculating the rear average value of each unit time, and judging the unit time which is likely to be blocked according to the change conditions of the front average value and the rear average value of each unit time; calculating a first variation between a front average value of the unit time of the possible hole blockage and a rear average value of the unit time of the possible hole blockage, comparing the first variation with standard deviations of average pulses of all unit time before the unit time of the possible hole blockage and/or after the unit time of the possible hole blockage, judging whether the unit time of the possible hole blockage occurs for the first time, if the unit time of the possible hole blockage occurs for the first time, reporting abnormality, and if the unit time of the possible hole blockage does not occur for the first time, continuing to execute the next step; after judging that the hole is likely to be blocked in the unit time, calculating the average value of the average pulse in all unit time of the current sampling, calculating a second variation between the average value of the average pulse in all unit time of the current sampling and the average value of the historical average pulse data, comparing the second variation with the standard deviation of the historical average pulse data, judging whether the hole is likely to be blocked in the unit time of the hole for the second time, reporting abnormality if the hole is likely to be blocked in the second time, and sending the average value of the average pulse in all unit time of the current sampling to a memory and storing the historical average pulse data if the hole is not blocked in the second time.
2. The impedance method detection system of claim 1, wherein the processor obtains historical averaged pulse data, averages and standard deviations of the historical averaged pulse data, comprising:
acquiring a linked list PreMPList of historical average pulse data, wherein the historical average pulse data is the average value of average pulses of all time units sampled in the past, acquiring a calculation formula of the average value of the historical average pulse data, calculating the average value MPre of the historical average pulse data, acquiring a calculation formula of standard deviation of the historical average pulse data, and calculating the standard deviation SPre of the historical average pulse data;
the calculation formula of the average value of the historical average pulse data comprises the following steps:
Figure FDA0004185035200000021
wherein n is the number of data in PreMPList;
the calculation formula of the standard deviation of the historical average pulse data comprises the following steps:
Figure FDA0004185035200000022
wherein n is the number of data in PreMPList.
3. The impedance method according to claim 1, wherein the processor obtains data of the voltage pulse analyzed by the sampling, counts an average pulse of cells passing through the aperture per unit time, and comprises:
acquiring total acquisition time T, acquiring unit time delta T, acquiring a calculation formula of total number N of unit time, and calculating total number N of unit time, wherein the calculation formula of total number N of unit time comprises:
N＝T/Δt，
Acquiring a formula for calculating the number of pulses in the ith unit time, and calculating the number of pulses in the ith unit time, wherein the formula for calculating the number of pulses in the ith unit time comprises the following steps:
m i ＝count(p(t,v))，
wherein, the value range of t is (i-1) delta t is less than or equal to t < i delta t, the value range of i is 0<i less than or equal to N, and i is a positive integer;
obtaining a calculation formula for calculating the average pulse of the ith unit time, and calculating the average pulse of the ith unit time, wherein the calculation formula for calculating the average pulse of the ith unit time comprises the following steps:
P i ＝(∑v)/m i ，
wherein v is p (t, v), the value range of t is (i-1) delta t is less than or equal to t < i delta t, the value range of i is 0<i less than or equal to N, and i is a positive integer;
the average pulse in each unit time is sequentially stored in the memory array P to obtain a memory array P= [ P ] 1 ,P 2 ,…,P i ,…,P N ]。
4. The impedance method detection system of claim 3, wherein said processor sequentially calculates a front average value for each unit time and sequentially calculates a rear average value for each unit time, comprising:
acquiring a memory group P, acquiring a calculation formula for calculating a front average value of each unit, and sequentially calculating a front average value S1 of each unit time i The calculation formula of the front average value of each unit comprises the following steps:
Figure FDA0004185035200000023
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
Acquiring a calculation formula for calculating the back average value of each unit, and sequentially calculating the back average value S2 of each unit time i The calculation formula of the post-mean value of each unit comprises the following steps:
Figure FDA0004185035200000031
wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring initialized linked List1 and linked List2, and obtaining the front average value S1 of each unit time i Is stored in a List1 to obtain a linked List1[ i ]]The post-average value S2 of each unit time i Is stored in a List2 to obtain a linked List2[ i ]]。
5. The impedance method according to claim 4, wherein the processor determines the unit time of possible plugging according to the change of the front average value and the rear average value of each unit time, comprising:
acquiring a List1[ i ] and a List2[ i ], acquiring a differential calculation formula, carrying out differential calculation on data in the List1[ i ], acquiring an initialized List DList1, storing the differential calculation result of the List1[ i ] into the List DList1 to obtain the List DList1[ i ], carrying out differential calculation on the data in the List2[ i ], acquiring an initialized List DList2, storing the differential calculation result of the List2[ i ] into the List DList2 to obtain the List DList2[ i ], wherein the differential calculation formula comprises:
DList1[i]＝List1[i+1]-List1[i]，
DList2[i]＝List2[i+1]-List2[i]，
Wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring a unit time judging formula of a possible hole blockage, wherein the unit time judging formula of the possible hole blockage comprises the following steps:
Dlist2[i]*Dlist1[i+1]<0，
traversing the linked list DList2[ i ], inputting the data in the linked list DList1[ i ] and the linked list DList2[ i ] into the unit time judgment formula of the possible hole blocking, and if the data accords with the judgment formula of the possible hole blocking, the (i+1) th unit time is the unit time of the possible hole blocking.
6. The impedance method according to claim 4, wherein the processor determines the unit time of possible plugging according to the change of the front average value and the rear average value of each unit time, comprising:
acquiring a List1[ i ] and a List2[ i ], acquiring a differential calculation formula, carrying out differential calculation on data in the List1[ i ], acquiring an initialized List DList1, storing the differential calculation result of the List1[ i ] into the List DList1 to obtain the List DList1[ i ], carrying out differential calculation on the data in the List2[ i ], acquiring an initialized List DList2, storing the differential calculation result of the List2[ i ] into the List DList2 to obtain the List DList2[ i ], wherein the differential calculation formula comprises:
DList1[i]＝List1[i+1]-List1[i]，
DList2[i]＝List2[i+1]-List2[i]，
Wherein, i is more than 0 and less than or equal to N-1, i is a positive integer;
acquiring a unit time judging formula of a possible hole blockage, wherein the unit time judging formula of the possible hole blockage comprises the following steps:
Dlist1[i]*Dlist2[i+1]<0，
traversing the linked list DList1[ i ], inputting the data in the linked list DList1[ i ] and the linked list DList2[ i ] into the unit time judgment formula of the possible hole blocking, and if the data accords with the judgment formula of the possible hole blocking, the (i+1) th unit time is the unit time of the possible hole blocking.
7. The impedance method detection system of claim 3, wherein said processor first determining whether a possible hole blockage has occurred per unit time comprises:
acquiring a record group P, acquiring a calculation formula of the average value of the average pulse of the first i unit time, wherein the i+1th unit time is the unit time which can block holes, and calculating the average value MP1 of the average pulse of the first i unit time, wherein the calculation formula of the average value of the average pulse of the first i unit time comprises:
Figure FDA0004185035200000041
wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a calculation formula of standard deviation of average pulse of the first i unit time, and calculating the standard deviation SP1 of the average pulse of the first i unit time, wherein the calculation formula of the standard deviation of the average pulse of the first i unit time comprises:
Figure FDA0004185035200000042
Wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a calculation formula of an average value of the average pulses in the (i+1) th to N unit time, and calculating an average value MP2 of the average pulses in the (i+1) th to N unit time, wherein the calculation formula of the average value of the average pulses in the (i+1) th to N unit time comprises:
Figure FDA0004185035200000043
wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a calculation formula of standard deviation of average pulses in the (i+1) th to N unit time, and calculating the standard deviation SP2 of the average pulses in the (i+1) th to N unit time, wherein the calculation formula of the standard deviation of the average pulses in the (i+1) th to N unit time comprises:
Figure FDA0004185035200000044
wherein, the value range of i is more than 0 and less than N, i is a positive integer;
acquiring a judging formula of first judgment, wherein the judging formula of the first judgment comprises:
|MP1-MP2|<SP1，
|MP1-MP2|<SP2，
and inputting the average value MP1 of the average pulse in the first i unit time, the standard deviation SP1 of the average pulse in the first i unit time, the average value MP2 of the average pulse in the i+1 to N unit time and the standard deviation SP2 of the average pulse in the i+1 to N unit time into a judging formula for the first judgment, wherein if at least one formula in the judging formula for the first judgment is established, the first judgment is that the hole is blocked, otherwise, the first judgment is that the hole is not blocked.
8. The impedance method detection system of claim 3, wherein said processor for a second time determining whether a possible hole blockage occurred per unit time comprises:
acquiring a memory group P, acquiring a calculation formula of the average value of the average pulse in all unit time of the current sample, and calculating the average value MP of the average pulse in all unit time of the current sample, wherein the calculation formula of the average value of the average pulse in all unit time of the current sample comprises:
Figure FDA0004185035200000051
acquiring a mean value MPre of the historical average pulse data and a standard deviation SPre of the historical average pulse data, acquiring a judgment formula of the second judgment, substituting MP, MPre and SPre into the judgment formula of the second judgment,
if the formula
|MPre-MP|<3SPre
If so, judging that the hole blockage does not occur for the second time;
if the formula
|MPre-MP|≥3SPre
If so, judging that the whole process hole blocking occurs for the second time.
9. A method of identifying a blocked orifice in an impedance channel, the method for detecting whether a blocked orifice has occurred in an impedance detection system of a blood cell analyzer, comprising:
acquiring historical average pulse data, and solving an average value and a standard deviation of the historical average pulse data;
acquiring the data of the voltage pulse analyzed by the sampling, and counting the average pulse of the cells passing through the small holes in each unit time;
Defining the average value of the average pulse in all unit time before each unit time as a front average value, sequentially calculating the front average value of each unit time, defining the average value of the average pulse in all unit time after each unit time as a rear average value, sequentially calculating the rear average value of each unit time, and judging the unit time which is likely to be blocked according to the change conditions of the front average value and the rear average value of each unit time;
calculating a first variation between a front average value of the unit time of the possible hole blockage and a rear average value of the unit time of the possible hole blockage, comparing the first variation with standard deviations of average pulses of all unit time before the unit time of the possible hole blockage and/or after the unit time of the possible hole blockage, judging whether the unit time of the possible hole blockage occurs for the first time, if the unit time of the possible hole blockage occurs for the first time, reporting abnormality, and if the unit time of the possible hole blockage does not occur for the first time, continuing to execute the next step;
after judging that the blocking of the holes is possible in the unit time for the first time, calculating the average value of the average pulse of all unit time of the current sampling, calculating a second variation between the average value of the average pulse of all unit time of the current sampling and the average value of the historical average pulse data, comparing the second variation with the standard deviation of the historical average pulse data, judging whether the blocking of the holes is possible in the unit time for the second time, if the blocking of the holes is possible in the second time, reporting the abnormality, and if the blocking of the holes is not possible in the second time, storing the average value of the average pulse of all unit time of the current sampling into the historical average pulse data.
10. A computer readable storage medium having stored thereon a program executable by a processor to implement the method of claim 9.
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