CN115877012A - Antigen concentration measuring method and measuring instrument - Google Patents
Antigen concentration measuring method and measuring instrument Download PDFInfo
- Publication number
- CN115877012A CN115877012A CN202111136358.3A CN202111136358A CN115877012A CN 115877012 A CN115877012 A CN 115877012A CN 202111136358 A CN202111136358 A CN 202111136358A CN 115877012 A CN115877012 A CN 115877012A
- Authority
- CN
- China
- Prior art keywords
- sample
- accommodating space
- antigen
- concentration
- measuring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000427 antigen Substances 0.000 title claims abstract description 176
- 102000036639 antigens Human genes 0.000 title claims abstract description 176
- 108091007433 antigens Proteins 0.000 title claims abstract description 176
- 238000000034 method Methods 0.000 title claims abstract description 50
- 238000005259 measurement Methods 0.000 claims abstract description 43
- 238000007865 diluting Methods 0.000 claims abstract description 28
- 238000010790 dilution Methods 0.000 claims description 100
- 239000012895 dilution Substances 0.000 claims description 100
- 238000006243 chemical reaction Methods 0.000 claims description 64
- 230000005540 biological transmission Effects 0.000 claims description 29
- 239000003085 diluting agent Substances 0.000 claims description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 230000036046 immunoreaction Effects 0.000 claims description 8
- 230000008105 immune reaction Effects 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- 239000000523 sample Substances 0.000 description 237
- 238000005070 sampling Methods 0.000 description 12
- 239000012470 diluted sample Substances 0.000 description 11
- 238000000691 measurement method Methods 0.000 description 7
- 230000004308 accommodation Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000004891 communication Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The application discloses an antigen concentration measuring method and an antigen concentration measuring instrument. The method comprises the following steps: diluting at least a portion of the first sample containing the antigen to obtain a second sample; measuring after the first accommodating space reacts with the second sample to determine whether the concentration of the antigen in the second sample exceeds the standard; if the concentration of the antigen in the second sample exceeds the standard, diluting to obtain a third sample containing the antigen; and measuring after the first accommodating space reacts to the third sample. By the above manner, the cost required for antigen concentration measurement can be reduced.
Description
Technical Field
The application relates to the technical field of clinical examination, in particular to an antigen concentration measuring method and an antigen concentration measuring instrument.
Background
In many applications, it is necessary to detect the concentration of the antigen contained in the sample. However, the existing detection conditions are not sufficient to support the measurement of the antigen concentration in all the numerical ranges, that is, when the antigen concentration contained in the sample exceeds the standard, the antigen concentration contained in the sample cannot be detected. Therefore, the method for detecting the concentration of the antigen contained in the sample comprises the steps of firstly measuring the concentration of the antigen contained in the sample in an overproof manner, and determining the concentration of the antigen contained in the sample under the condition that the concentration of the antigen is not overproof. The existing method for measuring the concentration of the antigen has high cost.
Disclosure of Invention
The application provides an antigen concentration measuring method and an antigen concentration measuring instrument, which can reduce the cost required by antigen concentration measurement.
In order to solve the technical problem, the application adopts a technical scheme that: an antigen concentration measuring method is provided. The method comprises the following steps: diluting at least a portion of the first sample containing the antigen to obtain a second sample; measuring after the first accommodating space reacts with the second sample to determine whether the concentration of the antigen in the second sample exceeds the standard; if the concentration of the antigen in the second sample exceeds the standard, diluting to obtain a third sample containing the antigen; and measuring after the first accommodating space reacts to the third sample.
In order to solve the above technical problem, another technical solution adopted by the present application is: the utility model provides a measuring instrument, this measuring instrument includes power component, measuring unit and control circuit, and is provided with first accommodation space, and control circuit connects power component and measuring unit. The control circuit is used for controlling the power assembly to dilute a first sample containing the antigen to obtain a second sample; controlling the measuring assembly to measure after the first accommodating space reacts with the second sample so as to determine whether the concentration of the antigen in the second sample exceeds the standard or not; if the concentration of the antigen in the second sample exceeds the standard, the control circuit is used for controlling the power assembly to dilute to obtain a third sample containing the antigen; and controlling the measuring assembly to measure after the first accommodating space reacts the third sample.
Through the mode, in the process of measuring the antigen concentration of the sample, the samples (the second sample and the third sample) obtained by different times of dilution are measured after reaction and are all in the first accommodating space. Therefore, the antigen concentration measurement can be realized only by one accommodating space for measurement after reaction in the whole measurement process. Therefore, the method provided by the application does not need to additionally add components in the existing measuring instrument, can fully utilize the existing measuring instrument to realize the antigen concentration measurement, and reduces the cost required by the antigen concentration measurement.
Drawings
FIG. 1 is a schematic view of a measuring instrument with a dilution cup built in;
FIG. 2 is another schematic view of the measuring apparatus with the dilution cup added inside the apparatus;
FIG. 3 is a schematic flow chart of a first embodiment of the method for measuring antigen concentration according to the present application;
FIG. 4 is a schematic flow chart of a second embodiment of the antigen concentration measurement method of the present application;
FIG. 5 is a schematic view of a measuring instrument according to the present application;
FIG. 6 is another schematic view of the measuring instrument of the present application;
FIG. 7 is a schematic view of another embodiment of the measuring instrument of the present application;
FIG. 8 is a schematic flow chart of a third embodiment of the antigen concentration measurement method of the present application;
FIG. 9 is a schematic flow chart of a fourth embodiment of the antigen concentration measurement method of the present application;
FIG. 10 is a schematic flow chart of a fifth embodiment of the method for measuring an antigen concentration according to the present application;
FIG. 11 is a schematic flow chart of a sixth embodiment of the antigen concentration measurement method of the present application;
FIG. 12 is a schematic flow chart showing an embodiment of the method for measuring an antigen concentration according to the present invention;
FIG. 13 is a schematic structural diagram of a first measurement apparatus according to the present application;
fig. 14 is a schematic structural diagram of a second measurement instrument embodiment of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only some embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The terms "first", "second" and "third" in this application are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any indication of the number of technical features indicated. Thus, a feature defined as "first," "second," or "third" may explicitly or implicitly include at least one of the feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless explicitly specifically limited otherwise.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments without conflict.
In many applications, it is necessary to measure the concentration of the antigen contained in the sample. However, existing assay conditions are not sufficient to support measurement of antigen concentrations over all ranges of values. In other words, when the concentration of the antigen in the sample exceeds a standard numerical range that can be measured (exceeds a standard), an antibody-containing reagent is added to the sample to cause the antigen in the sample to perform an immunoreaction with the antibody; obtaining a signal value in the sample after the immune reaction, wherein the antigen concentration in the sample obtained by converting the signal value is not the actual concentration.
Therefore, after the sample is diluted, it is necessary to determine whether the antigen concentration in the diluted sample exceeds the standard; carrying out secondary dilution on the sample under the condition of exceeding the standard, judging whether the antigen in the sample obtained by the secondary dilution exceeds the standard, \8230, and repeating the steps until the concentration of the antigen in the sample obtained by the last dilution does not exceed the standard; the concentration of the antigen in the sample obtained by the last dilution is the actual concentration, and therefore the concentration of the antigen in the original sample can be obtained based on the concentration of the antigen contained in the sample obtained by the last dilution and the dilution multiple of each time.
At present, methods for measuring the concentration of antigen in a sample generally adopt an external dilution mode, a pre-reaction mode or a mode of adding a dilution cup in an internal machine.
The external dilution mode is to store the sample outside the machine, dilute the antigen by adopting a certain dilution ratio, and then measure the concentration of the diluted sample. The process can not be completely automated, the workload of related personnel is increased, and the complexity of measuring the antigen concentration is high.
The pre-reaction mode needs to add antigen to each measured sample again, new antigen is added when the antigen concentration exceeds the standard, the measuring instrument further dilutes the re-injected sample, and then the antigen concentration exceeds the standard, the reagent consumption in the process is high, the measurement cost of a single sample is increased, the process control is complex, and the requirement on the measuring instrument is high.
The mode of adding the dilution cup in the machine can realize automatic measurement of the antigen concentration. The following describes the measurement procedure of adding a dilution cup in the machine with reference to fig. 1 and 2. The instrument comprises a plurality of dilution cups (1A and 2A for example) and a plurality of reaction pools (1B and 2B for example), 1) a sample is diluted for the Nth time in 1A by adding a diluent into 1A by using a sampling needle; 2) Utilizing a sampling needle to suck back a part from 1A to 1B, and determining whether the antigen concentration exceeds the standard in 1B; if the concentration of the antigen is not over standard, determining the concentration of the antigen; if the antigen concentration exceeds the standard, 3) utilizing a sampling needle to suck back the sample in the 1A to the 2A, and carrying out (N + 1) th dilution on the sample in the 2A; 4) Using a sampling needle to aspirate a portion from 2A to 2B, it was determined whether the antigen concentration was overproof in 2B \8230;. Thus, in this method, a plurality of dilution cups and a plurality of reaction cells need to be installed in the apparatus.
In conclusion, the existing antigen concentration measurement mode has high complexity and high cost.
In order to reduce the cost and complexity while realizing the measurement of the antigen concentration, the application provides an antigen concentration measurement method, which comprises the following steps:
FIG. 3 is a schematic flow chart of a first embodiment of the method for measuring antigen concentration according to the present application. It should be noted that, if the result is substantially the same, the flow sequence shown in fig. 3 is not limited in this embodiment.
As shown in fig. 3, the present embodiment may include:
s11: diluting at least a portion of the first sample containing the antigen to obtain a second sample.
The antigen concentration measuring method is applied to an instrument having an antigen concentration measuring function (hereinafter simply referred to as a measuring instrument), such as a specific protein reaction apparatus, and also, for example, an instrument including a specific protein reaction apparatus. The detailed description of the measuring instrument can be referred to the description of the structural embodiments below.
A first sample of the collected biological body may be placed in the measuring instrument in advance in a position for storing the first sample, and then at least part of the first sample is applied in this step.
All first samples can be diluted. Alternatively, only a portion of the first sample is diluted to achieve the reservation of the first sample. The reserved first sample may be used for subsequent S13.
The dilution factor for at least a portion of the first sample is known, such that the volume of dilution required to dilute at least a portion of the first sample can be determined based on the known dilution factor. Thus, the dilution of at least part of the first sample may be performed by adding at least part of the first sample and a corresponding volume of diluent to the holding space for dilution.
The accommodating space for dilution and the accommodating space for reaction (hereinafter referred to as the first accommodating space) may be the same or different. That is, the accommodating space for dilution may be the first accommodating space, may be a pipeline communicated with the first accommodating space, and may also be a dedicated dilution cup provided in the measurement instrument. Under the condition that the accommodating space for dilution is the first accommodating space, the measuring instrument does not need to be provided with an additional dilution cup, so that the cost required by the measurement of the concentration of the sample antigen can be reduced.
S12: and measuring after the first accommodating space reacts with the second sample to determine whether the concentration of the antigen in the second sample exceeds the standard or not.
In this step, all the second samples may be measured after reaction, or only a part of the second samples may be measured after reaction, thereby implementing the reservation of the second samples. The reserved second sample may be applied to S13.
If the accommodating space for dilution is the first accommodating space, the second sample may be reserved by moving a part of the second sample in the first accommodating space into the second accommodating space. If the accommodating space for dilution is not the first accommodating space, the second sample may be reserved in such a way that a part of the second sample is left in the accommodating space for dilution so that the part of the second sample does not participate in the reaction in the first accommodating space. The first accommodating space can be a reaction tank or other accommodating spaces for reaction.
If the concentration of the antigen in the second sample exceeds the standard, executing S13-S14; otherwise, S15 is executed.
S13: diluting to obtain a third sample containing antigen.
For simplicity of description, the sample that needs to be diluted when obtaining the third sample is hereinafter referred to as a dilution body. The diluting body may be a reserved first sample, a reserved second sample or other sample. The other sample is obtained by diluting the first sample, and the dilution factor of the other sample obtained by diluting the first sample is known.
Thus, in this step, at least another part of the first sample (the reserved first sample) may be diluted again to obtain a third sample; the second sample (the reserved second sample) can also be diluted again to obtain a third sample; other samples may also be diluted to obtain a third sample.
The dilution factor required to obtain the third sample (dilution factor against the dilution of the bulk) is known. Therefore, the volume of the diluent required by the third sample can be determined, and then the reserved first sample, the reserved second sample and other samples can be added into the accommodating space for dilution together with the diluent with the determined volume to obtain the third sample.
The dilution factor required to obtain the third sample may be the same as or different from the dilution factor required to obtain the second sample.
If the same, a fixed dilution factor may be set before the first dilution, after which the dilutions of different samples are performed according to the fixed dilution factor. In this way, if each dilution is performed based on the sample obtained from the previous dilution, the dilution factor is inversely related to the number of dilutions, and the concentration of antigen in the original sample (first sample) is positively related to the number of dilutions. After the first dilution, the number of times of dilution is 1 when the antigen concentration is not exceeded, and the number of times of dilution is greater than or equal to 2 when the antigen concentration is exceeded after the first dilution.
If different, the corresponding dilution multiple of each dilution can be preset, and each dilution is carried out according to the corresponding dilution multiple. Or, the dilution multiple of the next time can be determined based on the overproof result obtained by measurement after previous dilution and reaction, so that the antigen concentration in the sample obtained by the next dilution is not overproof, and the times of measurement after dilution and reaction are reduced.
S14: and measuring after the first accommodating space reacts to the third sample.
The manner of measuring after the reaction of the third sample is similar to the manner of measuring after the reaction of the second sample, and is not described herein again.
S15: determining the concentration of the antigen in the second sample.
Under the condition that the concentration of the antigen in the second sample does not exceed the standard, the dilution multiple when the second sample is obtained can be directly multiplied by the concentration of the antigen in the second sample to obtain the concentration of the antigen in the original sample (the first sample).
Through the implementation of this embodiment, this application is in the antigen concentration measurement process to the sample, and the sample (second sample and third sample) that obtains is measured after the reaction to different dilutions all in first accommodation space. Therefore, the antigen concentration measurement can be realized only by one accommodating space for measurement after reaction in the whole measurement process. Therefore, the method provided by the application does not need to additionally add components in the existing measuring instrument, can fully utilize the existing measuring instrument to realize the measurement of the antigen concentration, and reduces the cost required by the measurement of the antigen concentration.
In addition, in the case where the second sample is reserved before S12, the above-described first embodiment may be further extended to obtain the following second embodiment. The same parts as those in the first embodiment are not described in detail in this embodiment.
FIG. 4 is a schematic flow chart of a second embodiment of the antigen concentration measurement method of the present application. It should be noted that, if the result is substantially the same, the flow sequence shown in fig. 4 is not limited in this embodiment.
As shown in fig. 4, the present embodiment may include:
s21: and diluting at least part of the first sample in the first accommodating space to obtain a second sample.
The step limits the accommodating space for dilution to be the first accommodating space, so that the dilution and the reaction of the first sample are both in the first accommodating space.
The first sample can be added into the first accommodating space through the first transmission assembly, and the first sample can be added into the first accommodating space through the second transmission assembly, so that at least part of the first sample can be diluted. The first transmission component can be a diluent pipeline connected with the first accommodating space and can also be a sampling needle used for collecting diluent into the first accommodating space. The second transmission component can be a sample pipeline connected with the first accommodating space and can also be a sampling needle used for collecting the first sample into the first accommodating space.
S22: and moving part of the second sample in the first accommodating space into the second accommodating space.
The second receiving space may be any receiving space in the measuring instrument that can be used for storing the portion of the second sample.
However, in order to implement the second sample without additionally introducing a component into the measuring instrument, the first transmission component may be used as the second accommodating space, the second transmission component may be used as the second accommodating space, and a third transmission component (refer to the following description) may be used as the second accommodating space. Thus, in this step, a part of the second sample in the first accommodating space may be moved into at least one of the first transport assembly, the second transport assembly, and the third transport assembly as the second accommodating space.
Before the part of the second sample is moved into the second accommodating space, whether the second accommodating space meets the moving-in condition can be judged; if yes, moving the part of the second sample into a second accommodating space; if not, the part of the second sample is moved into the third accommodating space. The moving-in condition may include that the second accommodating space is empty, has been cleaned, is communicated with the first accommodating space, and the like.
In the case that there are a plurality of components that can be used as the second accommodating space in the measuring instrument, the candidate second accommodating space can be displayed on the user interface; and receiving a selection instruction, and moving part of the second samples into a second accommodating space pointed by the selection instruction. Specifically, the user may select a second accommodating space to be moved into from the candidate second accommodating spaces according to the requirement (capacity size, idle state, etc.), so as to generate a selection instruction, so as to control the portion of the second sample to be moved into the selected second accommodating space by using the selection instruction.
S23: and measuring after the first accommodating space reacts with the second sample to determine whether the concentration of the antigen in the second sample exceeds the standard or not.
If the concentration of the antigen in the second sample is exceeded, S23-S24 are performed.
S24: and emptying the first accommodating space.
The liquid in the first containing space can be emptied first, and then the first containing space is cleaned.
S25: and moving part of the second sample in the second accommodating space into the first accommodating space.
The reserved part of the second sample is moved into the first accommodating space again, so that the part of the second sample is diluted again in the first accommodating space to obtain a third sample, and then the third sample is measured after the third sample is reacted in the first accommodating space.
If part of the second sample in S22 is moved into the second accommodating space, the step is to move the part of the second sample in the second accommodating space back into the first accommodating space. If part of the second sample in S22 is moved into the third accommodating space, the step is to move part of the second sample in the third accommodating space into the first accommodating space again.
The second embodiment will be described below by way of two examples.
Example 1: the diluent is stored in the first storage pool a. The first transmission assembly is a first sampling needle, the first accommodating space is a reaction tank b, and the second accommodating space is a first sampling needle.
With reference to fig. 5-6, 1) a part of the diluent is sucked from the first storage pool a by the first sampling needle and added into the reaction pool b, so that after the first sample is subsequently added into the reaction pool b, the first sample is diluted by the diluent to obtain a second sample; 2) A part of second samples are sucked back by using the first sampling needle and stored, and the rest second samples in the reaction tank b are measured after reaction; 3) Emptying the reaction tank b under the condition that the concentration of the antigen exceeds the standard, and adding the second sample stored in the first sampling needle into the reaction tank b again; 4) And sucking part of the diluent from the first storage pool a by using a first sampling needle, adding the diluent into the reaction pool b to obtain a third sample, and measuring after the reaction of the third sample is carried out in the reaction pool b.
Example 2: the diluent is stored in the first storage pool a. The first transmission component is a conduit c, the first accommodating space is a reaction tank b, and the second accommodating space is a conduit c.
Referring to fig. 7, the first storage tank a and the reaction tank b are communicated through a conduit c, a first valve 1 is disposed on one side of the conduit c close to the first storage tank a, and a second valve 2 is disposed on one side close to the reaction tank b. Opening a first valve 1 and a second valve 2, adding the diluent in the first storage pool a into a reaction pool b, closing the first valve 1 after a preset time (depending on the dilution times), and diluting the first sample in the reaction pool b by using the diluent to obtain a second sample after the first sample is added into the reaction pool b subsequently; sucking part of the second sample in the reaction cell b, storing the part of the second sample in a pipeline between the first valve 1 and the second valve 2 of the conduit c, and closing the second valve 2; measuring after reacting the remaining second sample in the reaction tank b; emptying the reaction tank b under the condition that the antigen concentration is determined to be over standard; opening the second valve 2 to add the second sample stored in the line between the first valve 1 and the second valve 2 to the reaction cell b again; and keeping the second valve 2 open, opening the first valve 1, adding the diluent in the first storage pool a into the reaction pool b to dilute the second sample by using the diluent to obtain a third sample, and measuring after the reaction of the third sample is carried out in the reaction pool b.
As mentioned in the above description related to S12, the dilution factor of the next time can be determined based on the result of the overproof measurement after the previous dilution and reaction. In this case, it is anticipated that the antigen concentration in the third sample will be exceeded, and the first example described above can be extended to the third example below.
FIG. 8 is a schematic flow chart of a third embodiment of the antigen concentration measurement method of the present application. It should be noted that, if the result is substantially the same, the flow sequence shown in fig. 8 is not limited in this embodiment. In this embodiment, S31 is a step that may be included before S13, S32 is a further extension of S13, and S33 is a further extension of S14. As shown in fig. 8, the present embodiment may include:
s31: and determining the dilution factor required for obtaining the third sample based on the result that the concentration of the antigen in the second sample exceeds the standard.
If the concentration of the antigen in the second sample exceeds the standard, the amount of the antigen in the second sample exceeding the standard can be determined based on the measurement result, and further, the concentration of the antigen in the third sample is predicted not to exceed the standard, and the dilution multiple required by the third sample is obtained.
S32: and diluting by using a dilution factor to obtain a third sample.
In a case where the accommodating space for dilution is the first accommodating space, a volume of the diluent required to obtain the third sample may be determined based on the dilution multiple and the volume of the dilution body, and the determined volume of the diluent and the dilution body may be added to the first accommodating space to obtain the third sample.
Considering that the capacity of the first accommodating space is limited, if the volume of the diluent required for obtaining the third sample is determined directly based on the dilution factor and the volume of the dilution body, the first accommodating space may be insufficient to accommodate the obtained third sample, and the first accommodating space may overflow. For this reason, can restrict to add the volume of diluting the main part to first accommodation space to the volume of the diluent that the dilution main part dilution needs to add first accommodation space is restricted, and then the volume of the third sample that obtains is restricted.
Taking the dilution main body as at least another part of the first sample or the second sample as an example, the volume of adding at least another part of the first sample or the second sample to the first accommodating space can be determined based on the dilution factor under the condition that the first accommodating space is not overflowed; and adding at least another part of the first sample or the second sample into the first accommodating space according to the determined volume, and adding diluent into the first accommodating space to obtain a third sample.
S33: and measuring after the first accommodating space reacts to all the third samples.
In the case where the third sample is obtained by dilution with the dilution factor determined in S31, the antigen concentration in the third sample does not exceed the standard. Thus, there is no need to reserve a third sample for the next dilution. Further, this step can perform post-reaction measurements on all third samples.
Through the implementation of the embodiment, the dilution multiple can be determined based on the result that the antigen concentration of the second sample exceeds the standard, and the antigen concentration of the third sample obtained by diluting the second sample according to the determined dilution multiple does not exceed the standard, so that the antigen concentration contained in the third sample can be directly determined, the concentration of the original sample is further determined, and circulating dilution, reaction and measurement are not needed to be carried out again, so that the cost required by antigen concentration measurement can be saved, and the measurement complexity is reduced.
If the dilution multiple of the next time is not determined based on the overproof result obtained by the previous dilution and measurement after the reaction, in this case, whether the antigen concentration in the third sample is overproof or not is unpredictable, the above embodiment can be further expanded to obtain the following embodiment four.
FIG. 9 is a schematic flow chart of a fourth embodiment of the method for measuring an antigen concentration according to the present application. It should be noted that, if the result is substantially the same, the flow sequence shown in fig. 9 is not limited in this embodiment. S41 in this embodiment is a step that may be included before S14, and S42 is a further extension of S14. As shown in fig. 9, the present embodiment may include:
s41: and moving part of the third sample into the second accommodating space.
Considering that whether the concentration of the antigen contained in the third sample exceeds the standard is unknown, before the first accommodating space reacts on the third sample, a part of the third sample can be moved into the second accommodating space, so that the third sample is reserved. The reserved third sample can be used for the next dilution.
If the second sample is reserved in the second accommodating space, the second accommodating space can be cleaned before part of the third sample is moved into the second accommodating space.
S42: and measuring after the first accommodating space reacts the third sample remained after the third sample is moved into the second accommodating space.
In addition, after S14, it can be determined whether the concentration of the antigen in the third sample exceeds the standard based on the result of measurement after the reaction of the third sample, so that the first embodiment can be extended to the fifth embodiment.
FIG. 10 is a schematic flow chart of a fifth embodiment of the method for measuring an antigen concentration according to the present invention. It should be noted that, if the result is substantially the same, the flow sequence shown in fig. 10 is not limited in this embodiment. S41 in this embodiment is a step that may be included before S14, and S42 is a further extension of S14. As shown in fig. 10, the present embodiment may include:
s51: determining whether the concentration of the antigen in the third sample is overproof.
If the antigen concentration in the third sample exceeds the standard, executing S52; otherwise, S53 is executed.
S52: a fourth sample containing the antigen is obtained by dilution and is used instead of the third sample.
After the step is executed, jumping to S51, and repeatedly executing the steps until the antigen concentration detected for the last time does not exceed the standard.
S53: determining the concentration of the antigen in the third sample.
For other detailed descriptions in this embodiment, please refer to other embodiments, which are not repeated herein.
In addition, the above-described process of determining whether the antigen concentration is out of limits is similar to that of the second and third samples after the reaction. The following describes the procedure of the measurement after the reaction in detail by taking the second sample as an example in example six.
FIG. 11 is a schematic flow chart of a sixth embodiment of the method for measuring an antigen concentration according to the present application. It should be noted that, if the result is substantially the same, the flow sequence shown in fig. 11 is not limited in this embodiment. As shown in fig. 11, the present embodiment may include:
s61: and adding a reagent containing the antibody into the first accommodating space through the third transmission assembly so as to enable the antigen contained in the second sample to perform immunoreaction with the antibody.
The third transmission component can be a reagent pipeline communicated with the first accommodating space and can also be a reagent needle used for collecting a reagent containing the antibody.
S62: and carrying out antigen detection on the second sample after the immune reaction so as to determine whether the antigen concentration exceeds the standard.
A signal value corresponding to the second sample after the immune reaction can be collected; based on the signal value, it is determined whether the antigen concentration is overproof. The signal values may be optical signal values or electrical signal values.
It will be appreciated that the method described above for determining whether the antigen in the second sample is overproof is immuno-nephelometry. The immune scattering turbidimetry is a phenomenon that an antigen and an antibody are combined to generate a specific reaction (immune reaction) to form an insoluble complex, according to a Heidelberg curve, when the antibody exceeds the standard (the antigen does not exceed the standard), the concentration of the formed insoluble complex is in a direct proportion relation with the concentration of the antigen, and the signal intensity is in a direct proportion relation with the concentration of the insoluble complex, so that the signal intensity is obtained and is in a direct proportion relation with the concentration of the antigen. Therefore, whether the antigen exceeds the standard or not can be determined through the signal intensity.
The method provided by the present application is described below by way of a specific example with reference to fig. 12.
Example 3:
1) Adding a sample (original sample) containing the antigen into the reaction pool, and performing primary dilution treatment on the sample in the reaction pool to obtain a primary diluted sample;
2) Transferring part of the primary diluted sample to other units (diluent pipelines) outside the reaction cell, and performing immunoreaction on the rest primary diluted sample in the reaction cell to determine whether the antigen concentration in the primary diluted sample is excessive/overproof; if the antigen concentration in the sample of one dilution is excessive, entering 3); if the antigen concentration in the sample diluted for the first time is not excessive, determining the antigen concentration;
3) Adding the primary diluted sample in the diluent pipeline into a reaction tank and diluting to obtain a secondary diluted sample;
4) Transferring part of the secondary diluted sample to other units outside the reaction cell, and performing an immunoreaction on the remaining secondary diluted sample in the reaction cell to determine whether the antigen concentration in the secondary diluted sample is excessive.
Fig. 13 is a schematic structural diagram of a first embodiment of the measuring instrument according to the present application. As shown in fig. 13, the measuring instrument 1 is used for antigen concentration measurement of a sample. As shown in fig. 12, the measuring instrument 1 may include a power assembly 11, a measuring assembly 12, and a control circuit 13, and is provided with a first accommodating space 14 and a second accommodating space 15, and the control circuit 13 connects the power assembly 11 and the measuring assembly 12.
The control circuit 13 may be configured to control the power assembly 11 to dilute the first sample containing the antigen to obtain a second sample; and controlling the measuring component 12 to measure after the first accommodating space reacts on the second sample so as to determine whether the concentration of the antigen in the second sample exceeds the standard.
If the concentration of the antigen in the second sample is out of the standard, the measurement result of the measurement component 12 is the concentration of the antigen in the second sample. If the concentration of the antigen in the second sample exceeds the standard, the control circuit 13 is also used for controlling the power assembly 11 to dilute to obtain a third sample containing the antigen; and controlling the measuring assembly 12 to measure after the first accommodating space 14 reacts to the third sample. The dilution body when the third sample is obtained may be at least another part of the first sample or the second sample, or may be another sample. In case the dilution body is at least another part of the first sample or the second sample, the control circuit 13 may be further configured to control the power assembly 11 to perform a further dilution of the at least another part of the first sample or the second sample to obtain a third sample.
Further, the dilution and the post-reaction measurement of the sample can be performed in the first accommodating space 14. In this case, the control circuit 13 may be configured to control the power assembly 11 to dilute the first sample in the first accommodating space to obtain a second sample; before the measuring assembly 12 is controlled to measure after the first accommodating space 14 reacts to the second sample, the power assembly 11 is controlled to move a part of the second sample in the first accommodating space 14 into the second accommodating space 15.
Fig. 14 is a schematic structural diagram of a second measurement instrument embodiment of the present application. As shown in fig. 14, on the basis of the first embodiment, the measuring instrument 1 may further include a first transmission assembly 151, a second transmission assembly 151, and a third transmission assembly 153. The first transfer unit 151, the second transfer unit 151, and the third transfer unit 153 may be used as the second receiving space 15.
The first transport assembly 151 may be used to transport the diluent and the second transport assembly 152 may be used to transport the first sample. The control circuit 13 may be configured to control the power assembly 11 to add the diluent to the first accommodating space 14 via the first transporting assembly 151, and add the first sample to the first accommodating space 14 via the second transporting assembly 152.
The third transport assembly 153 may be used to transport antibody-containing reagents. The control circuit 13 may be configured to control the power assembly 11 to add a reagent containing an antibody into the first accommodating space 14 via the third transmission assembly 153, so that an antigen contained in the second sample and the antibody perform an immunoreaction; and controlling the measuring component 12 to carry out antigen detection on the second sample after the immune reaction so as to determine whether the antigen concentration exceeds the standard.
For the structural embodiments of the measuring instrument 1, reference is not made in any detail to the preceding method embodiments.
In the several embodiments provided in the present application, it should be understood that the disclosed system, apparatus and method may be implemented in other manners. For example, the above-described apparatus embodiments are merely illustrative, and for example, a division of a unit is merely a logical division, and an actual implementation may have another division, for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, devices or units, and may be in an electrical, mechanical or other form.
In addition, functional units in the embodiments of the present application may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, and can also be realized in a form of a software functional unit. The above embodiments are merely examples and are not intended to limit the scope of the present disclosure, and all modifications, equivalents, and flow charts using the contents of the specification and drawings of the present disclosure or those directly or indirectly applied to other related technical fields are intended to be included in the scope of the present disclosure.
Claims (17)
1. A method for measuring the concentration of an antigen, comprising:
diluting at least a portion of the first sample containing the antigen to obtain a second sample;
measuring after the first accommodating space reacts on the second sample to determine whether the concentration of the antigen in the second sample exceeds a standard or not;
if the concentration of the antigen in the second sample exceeds the standard, diluting to obtain a third sample containing the antigen;
and measuring after the first accommodating space reacts to the third sample.
2. The method of claim 1,
said diluting to obtain a third sample containing said antigen comprises:
and diluting at least another part of the first sample or the second sample again to obtain the third sample.
3. The method of claim 1,
said diluting at least a portion of the first sample containing said antigen to obtain a second sample comprises:
diluting at least part of the first sample in the first accommodating space to obtain a second sample;
the first accommodating space comprises the following components before the measurement after the reaction of the second sample is carried out on the first accommodating space:
and moving part of the second sample in the first accommodating space into a second accommodating space.
4. The method of claim 3,
the diluting at least a portion of the first sample containing the antigen to obtain a second sample comprises:
adding a diluent into the first accommodating space through a first transmission assembly, and adding at least part of the first sample into the first accommodating space through a second transmission assembly;
the moving part of the second sample in the first accommodating space into the second accommodating space comprises:
and moving part of the second sample in the first accommodating space into the first transmission assembly and/or the second transmission assembly serving as the second accommodating space.
5. The method of claim 3,
before the dilution to obtain a third sample containing the antigen, the method comprises:
emptying the first accommodating space;
moving the portion of the second sample of the second volume into the first volume.
6. The method of claim 3,
the measuring after the reaction of the second sample in the first accommodating space to determine whether the concentration of the antigen in the second sample exceeds the standard comprises:
adding a reagent containing an antibody into the first accommodating space through a third transmission assembly so as to enable an antigen contained in the second sample to perform an immunoreaction with the antibody;
performing antigen detection on the second sample after the immune reaction to determine whether the antigen concentration exceeds a standard or not;
said moving said portion of said second sample of said first volume into a second volume comprises:
and moving part of the second sample in the first accommodating space into the third transmission assembly serving as the second accommodating space.
7. The method of claim 3,
the moving part of the second sample in the first accommodating space into the second accommodating space comprises:
judging whether the second accommodating space meets the moving-in condition or not;
if yes, moving the part of the second sample into the second accommodating space;
and if not, moving the part of the second sample into the third accommodating space.
8. The method of claim 3,
the moving part of the second sample in the first accommodating space into the second accommodating space comprises:
displaying the candidate second accommodating space on a user interface;
and receiving a selection instruction, and moving the part of the second samples into a second accommodating space pointed by the selection instruction.
9. The method of claim 1,
before said diluting results in a diluted third sample containing said antigen comprising:
determining the dilution factor required for obtaining the third sample based on the result that the concentration of the antigen in the second sample exceeds the standard;
said diluting resulting in a diluted third sample containing said antigen comprising:
diluting by using the dilution times to obtain the third sample;
the measuring after the first accommodating space reacts to the third sample includes:
and measuring after the first accommodating space reacts to all the third samples.
10. The method of claim 9, wherein prior to said diluting to obtain a third sample comprising said antigen, comprising:
adding at least another part of the first sample or the second sample into a first accommodating space, and adding a diluent into the first accommodating space;
prior to said diluting to obtain a diluted third sample containing said antigen comprising:
determining a volume of the at least another portion of the first sample or the second sample to be added to the first volume based on the dilution factor without causing the first volume to overflow.
11. The method of claim 1, wherein prior to the post-reaction measurement of the third sample in the first receiving space, comprising:
moving part of the third sample into the second accommodating space;
the measuring after the first accommodating space reacts to the third sample includes:
and measuring after the first accommodating space reacts the third sample remained after the third sample is moved into the second accommodating space.
12. The method of claim 1, wherein after the post-reaction measurement of the third sample in the first receiving space, the method further comprises:
determining whether the concentration of the antigen in the third sample is overproof;
determining the concentration of antigen in the third sample if the concentration of antigen in the third sample is not out of tolerance.
13. The method of claim 12, further comprising:
if the concentration of the antigen in the third sample exceeds the standard, diluting to obtain a fourth sample containing the antigen;
and replacing the third sample with the fourth sample, and skipping to the step of determining whether the antigen concentration in the third sample exceeds the standard or not until the antigen concentration detected for the last time does not exceed the standard.
14. A measuring instrument is characterized by comprising a power assembly, a measuring assembly and a control circuit, wherein a first accommodating space is arranged in the measuring instrument, and the control circuit is connected with the power assembly and the measuring assembly;
the control circuit is used for controlling the power assembly to dilute a first sample containing the antigen to obtain a second sample; controlling the measuring assembly to measure after the first accommodating space reacts on the second sample so as to determine whether the concentration of the antigen in the second sample exceeds a standard or not;
if the concentration of the antigen in the second sample exceeds the standard, the control circuit is used for controlling the power assembly to dilute to obtain a third sample containing the antigen; and controlling the measuring component to measure after the first accommodating space reacts to the third sample.
15. The apparatus of claim 14,
the control circuit is used for controlling the power assembly to dilute at least another part of the first sample or the second sample again to obtain the third sample.
16. The apparatus of claim 14,
the measuring instrument is provided with a second accommodating space;
the control circuit is specifically configured to control the power assembly to dilute the first sample in the first accommodating space to obtain the second sample; and before the first accommodating space reacts with the second sample and then controls the measuring assembly to measure, controlling the power assembly to move part of the second sample in the first accommodating space into the second accommodating space.
17. The apparatus of claim 14,
the measuring instrument comprises a first transmission assembly and a second transmission assembly;
the control circuit is specifically used for controlling the power assembly to add diluent into the first accommodating space through the first transmission assembly and add the first sample into the first accommodating space through the second transmission assembly; the first transmission assembly or the second transmission assembly is used as the second accommodating space; or
The measuring instrument comprises a third transmission assembly used as the second accommodating space;
the control circuit is specifically used for controlling the power assembly to add a reagent containing an antibody into the first accommodating space through the third transmission assembly so as to enable an antigen contained in the second sample to perform immunoreaction with the antibody; controlling the measuring component to carry out antigen detection on the second sample after the immunoreaction so as to determine whether the antigen concentration exceeds a standard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111136358.3A CN115877012A (en) | 2021-09-27 | 2021-09-27 | Antigen concentration measuring method and measuring instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111136358.3A CN115877012A (en) | 2021-09-27 | 2021-09-27 | Antigen concentration measuring method and measuring instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115877012A true CN115877012A (en) | 2023-03-31 |
Family
ID=85762974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111136358.3A Pending CN115877012A (en) | 2021-09-27 | 2021-09-27 | Antigen concentration measuring method and measuring instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115877012A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH045568A (en) * | 1990-04-23 | 1992-01-09 | Anariiteikaru Instr:Kk | Method for measuring specimen |
| JP2011069755A (en) * | 2009-09-28 | 2011-04-07 | Aloka Co Ltd | Method and device for immunoassay |
| CN103185779A (en) * | 2011-12-30 | 2013-07-03 | 深圳市亚辉龙生物科技有限公司 | Reagent device for detecting antinuclear antibody and method thereof |
| CN109470870A (en) * | 2016-11-22 | 2019-03-15 | 博阳生物科技（上海）有限公司 | Method of immunity, the system for identifying immunoassays and kit |
| CN110609139A (en) * | 2018-06-14 | 2019-12-24 | 深圳市理邦精密仪器股份有限公司 | Antigen concentration excess detection method, device and storage medium |
| US20200116710A1 (en) * | 2017-06-26 | 2020-04-16 | Bio-Rad Europe Gmbh | In situ serial dilution method |
| CN111089955A (en) * | 2018-10-24 | 2020-05-01 | 深圳迈瑞生物医疗电子股份有限公司 | Substance concentration determination method, sample analyzer and storage medium |
| CN113125785A (en) * | 2021-03-29 | 2021-07-16 | 深圳市科曼医疗设备有限公司 | Method for detecting high-concentration sample and calling time sequence |
-
2021
- 2021-09-27 CN CN202111136358.3A patent/CN115877012A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH045568A (en) * | 1990-04-23 | 1992-01-09 | Anariiteikaru Instr:Kk | Method for measuring specimen |
| JP2011069755A (en) * | 2009-09-28 | 2011-04-07 | Aloka Co Ltd | Method and device for immunoassay |
| CN103185779A (en) * | 2011-12-30 | 2013-07-03 | 深圳市亚辉龙生物科技有限公司 | Reagent device for detecting antinuclear antibody and method thereof |
| CN109470870A (en) * | 2016-11-22 | 2019-03-15 | 博阳生物科技（上海）有限公司 | Method of immunity, the system for identifying immunoassays and kit |
| US20200116710A1 (en) * | 2017-06-26 | 2020-04-16 | Bio-Rad Europe Gmbh | In situ serial dilution method |
| CN110609139A (en) * | 2018-06-14 | 2019-12-24 | 深圳市理邦精密仪器股份有限公司 | Antigen concentration excess detection method, device and storage medium |
| CN111089955A (en) * | 2018-10-24 | 2020-05-01 | 深圳迈瑞生物医疗电子股份有限公司 | Substance concentration determination method, sample analyzer and storage medium |
| CN113125785A (en) * | 2021-03-29 | 2021-07-16 | 深圳市科曼医疗设备有限公司 | Method for detecting high-concentration sample and calling time sequence |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3031242B2 (en) | Multi-item analyzer | |
| US6776961B2 (en) | Workstation for integrating automated chemical analyzers | |
| CN103884851A (en) | System for managing bulk liquids and/or solids | |
| US8939040B2 (en) | Analyzing apparatus and analyzing method | |
| JP7203139B2 (en) | Analysis method | |
| EP3139222B1 (en) | Analytical test management system and method | |
| JP2008281453A (en) | Automatic analysis system | |
| EP2418494A1 (en) | Autoanalyzer | |
| CN109975568A (en) | Sample rack dispatch control method, system and Medical Equipment | |
| CN111855985A (en) | Analysis meter | |
| CN111279198B (en) | Automatic analyzer | |
| CN115877012A (en) | Antigen concentration measuring method and measuring instrument | |
| CN102103146A (en) | System and process for analyzing liquid sample | |
| JP3626032B2 (en) | Automatic analysis method and automatic analyzer | |
| JP2011017660A (en) | Automatic analysis device and retesting method | |
| CN211652912U (en) | Full-automatic biochemical analyzer | |
| CN113777332B (en) | Immunoassay instrument and autoimmune analysis method | |
| EP3913373B1 (en) | Automatic analysis system and automatic analysis method for analytes | |
| CN115524495A (en) | Sample analysis device and method | |
| CN107516014B (en) | Method for the metrological processing of qualitative and quantitative measurement data | |
| US20220326271A1 (en) | Methods for onboard dilutions using automated analyzer | |
| JP2753340B2 (en) | Preparation of reaction sample | |
| EP4296685A1 (en) | Automatic analysis device and method for suctioning sample in automatic analysis device | |
| US20240019454A1 (en) | Integrated automated analyzer and methods of analyzing whole blood and plasma from a single sample tube | |
| EP1154274A2 (en) | Method of assaying a specimen using a reagent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |