JP2753340B2 - Preparation of reaction sample - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to an improvement in a method for preparing a reaction sample in an analyzer for biochemistry or immunology.

[Prior Art] When analyzing a sample of a certain sample by an automatic analyzer for biochemistry or immunology, a diluted sample may be used depending on an analysis item. Also, diluted samples are often used for re-examination of abnormally high samples and urinalysis.

In this conventional analysis method using an automatic analyzer, a diluted sample to be used is prepared by dispensing a fixed amount of an original sample and a diluent into a sample cup via a transfer device such as a syringe, and mixing them with stirring. The diluted sample prepared in the sample cup in the process of the above is dispensed into a required number of reaction vessels by another transfer device, and further, a predetermined reagent is dispensed into each reaction vessel and a reaction sample is produced by mixing and mixing, These reaction samples were analyzed and quantified by light transmittance measurement or the like.

[Problems to be Solved by the Invention] However, the conventional analysis method has the following problems.

a. In addition to the sample cup for the original sample and the reaction vessel for the reaction sample as the sample cup, a diluted sample cup for dilution as an intermediate treatment was required, which required space and cost.

b.Because there is a dilution process as an intermediate process, separate transfer devices are required for transfer between the sample cup and the diluted sample cup and for transfer between the diluted sample cup and the reaction vessel, and in this regard, a large space is required. And needed expense. Attempting to save space and expense and to use a common transfer device has the further problem of increasing the analysis time due to the series of operations.

c. The conventional analysis method dispenses the diluted sample in the diluted sample cup into the required number of reaction vessels, so the dilution ratio of the diluted sample supplied to each reaction vessel is the same, and When reaction samples having different dilution ratios are required, it is necessary to prepare diluted sample cups corresponding to different dilution ratios, and the apparatus becomes complicated.

d. In addition, two steps of quantitative dispensing are required before dispensing the original sample into the reaction vessel, during which the probe of the dispenser is affected twice by the carryover, which deteriorates the analysis accuracy. There was fear.

The present invention has been made in order to solve the problems of the conventional method as described above, and it is an object of the present invention to provide a method for preparing a reaction sample which enables simplification of an apparatus and reduces the influence of carryover. The purpose is.

[Means for Solving the Problems] In a method for preparing a reaction sample for analysis, in which a sample of a sample used in an analyzer for biochemistry or immunology is diluted with a diluent and a reagent is added, a sample and a diluent are desired. In accordance with the dilution factor corresponding to the analysis item to be analyzed, each predetermined amount is directly supplied to the reaction vessel of the analysis item and mixed to prepare a diluted sample having a desired dilution ratio. The method is characterized in that an excess amount is discharged from the reaction vessel while leaving the determined required amount, and a predetermined reagent is added to and mixed with the required amount of the diluted sample left in the reaction vessel.

[Operation] By directly preparing the reaction sample in the reaction vessel, the diluted sample cup can be omitted, and the chance of contamination due to carryover accompanying the dispensing operation can be reduced.

Embodiment An embodiment of the present invention will be described below with reference to the drawings. FIGS. 1 (a) to 1 (g) are explanatory diagrams of a method sequence from preparation of a diluted sample to analysis and quantification of the present invention.

In the figure, reference numeral 1 denotes a reaction vessel, and a required number of reaction vessels 1 are prepared in correspondence with the analysis items of the specimen and the dilution ratios required by the analysis items, and the first number shown in FIG. In the process, a diluent 2 such as a physiological saline solution is dispensed into these reaction vessels 1 by a dispenser (not shown) in a predetermined amount according to a required dilution ratio. Next, in a second step shown in FIG. 4B, a sample 3 of the subject is placed in a reaction vessel 1 containing a diluent 2 by a dispenser (not shown) in accordance with a required dilution ratio. The diluent is dispensed and added, and in a third step shown in FIG. 3C, the diluent 2 and the sample 3 are uniformly mixed by an operation such as stirring to prepare a diluted sample 4 having a required dilution ratio.

When the diluted sample 4 has been prepared, in a fourth step shown in FIG. 4D, an amount of the diluted sample 4 necessary for analysis is left from the reaction vessel 1 and an extra diluted sample 4 is dispensed by a dispenser or the like. Then, in a fifth step shown in FIG. 11E, a predetermined reagent 5 corresponding to the analysis item is dispensed by a dispenser (not shown). In step 6, the diluted sample 4 and the reagent 5 are uniformly mixed by an operation such as stirring, and reacted at a constant temperature to prepare a reaction sample 6.

When the reaction sample 6 has been prepared, the subject sample 3 is analyzed and quantified by a photometric test or the like for measuring the light transmittance of the reaction sample 6 in a seventh step shown in FIG.

In this embodiment, the same operation and effect can be obtained even if the order of the first step and the second step is reversed.

The present invention is not limited to the above-described embodiment, and can be modified and implemented without departing from the scope of the invention.

According to the present invention, the following effects can be expected.

a. Since the diluted sample is prepared directly in the reaction vessel, a sample cup for the diluted sample and its installation space and a transfer device for transferring the diluted sample to the reaction vessel are not required.

b. The method according to the present invention requires only the discharge operation to prepare the diluted sample, reduces the influence of contamination due to carry-over in the probe and reduces the analysis accuracy compared to the conventional method having a suction and discharge step. Can be increased.

c. Since the diluted sample is prepared directly in the reaction container, it is easy to prepare a diluted sample having a dilution ratio suitable for the analysis item for each reaction container.

[Brief description of the drawings]

1 (a) to 1 (g) are explanatory diagrams of a method sequence from preparation of a diluted sample to analysis and quantification according to one embodiment of the present invention. 1 ... reaction vessel 2 ... diluent 3 ... sample 4 ... diluted sample 5 ... reagent, 6 ... reaction sample

Claims (1)

(57) [Claims] 1. A method for preparing a reaction sample for analysis, comprising diluting a sample of a specimen to be used in an analyzer for biochemistry and immunology with a diluent and adding a reagent thereto, wherein a desired analysis of the sample and the diluent is performed. According to the dilution ratio corresponding to the item, each predetermined amount is directly supplied to the analysis item corresponding to the analysis item, the reaction container for analysis corresponding to the dilution ratio, and mixed to prepare a diluted sample having the desired dilution ratio. A reaction characterized by leaving a required amount determined for each analysis item in the sample, discharging an excess amount from the reaction vessel, adding a predetermined reagent to a required amount of the diluted sample left in the reaction vessel, and mixing the diluted sample to prepare. Sample preparation method.