CN102901835A - Method and device for collecting sample by full-automatic blood cell analyzer - Google Patents
Method and device for collecting sample by full-automatic blood cell analyzer Download PDFInfo
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- CN102901835A CN102901835A CN2012103896337A CN201210389633A CN102901835A CN 102901835 A CN102901835 A CN 102901835A CN 2012103896337 A CN2012103896337 A CN 2012103896337A CN 201210389633 A CN201210389633 A CN 201210389633A CN 102901835 A CN102901835 A CN 102901835A
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Abstract
The invention discloses a method and a device for collecting a sample by a full-automatic blood cell analyzer, belongs to a blood detection device, and particularly relates to a method and a device for collecting the sample by the full-automatic blood cell analyzer used in a blood routine test. The device comprises a sampling needle, and an inner hole of the sampling needle is connected with a diluent injection device through a pipeline. The method is characterized by comprising the steps that a sampling needle outer wall cleaning element is sleeved outside the sampling needle; a waste fluid outlet and a diluent inlet for cleaning in up and down arrangement are arranged at the lateral wall of a housing of the sampling needle outer wall cleaning element; the waste liquid outlet is connected with a waste liquid collecting device, and the diluent inlet for cleaning is connected with a diluent supply device. The sampling needle is full of diluent; the diluent and a whole blood sample are isolated by an air column; the outer wall of the sampling needle is cleaned by the sampling needle outer wall cleaning element; the front end of the whole blood sample is removed before the whole blood sample is injected; and the inner wall of the sampling needle is cleaned after the whole blood sample is injected, so that the metering accuracy for sample collection is ensured.
Description
Technical field
The invention belongs to blood testing equipment, method and the device of the sample collection of the Automatic Blood Cell Analyzer that particularly uses in the clinical examination routine blood test project.
Background technology
The Automatic Blood Cell Analyzer that uses in the clinical examination routine blood test project requires trace blood, three classification or five classification cellanalyzers approximately need to use 10-60 microlitre whole blood sample to measure, so the sample collection of trace is easy to occur following problem: 1. be present in the air in the sampling probe pipeline, in the syringe pump sample drawn, because the existence of pressure reduction, the cumulative volume of air can change, the quantity that affects sample collection is inaccurate, 2. each extract and during the filling sample, the amount of sampling probe inwall or the residual sample of outer wall is inconsistent, can cause the inaccurate problem of application of sample, if 3. the sampling probe inwall is full of dilution, after diluting liquid level and the sample liquid level contacting, can mutually permeate, change the concentration of whole blood sample, when whole blood sample need to be filled into respectively different measuring mechanisms, whole blood sample can lengthen in the sampling probe internal stops time, and along with each filling whole blood sample, it is also just more serious that infiltration occurs mutually for cleaning fluid and whole blood sample, caused the concentration of whole blood sample of each filling inconsistent.
Summary of the invention
In order to overcome in existing three classification or the five classification cellanalyzer sample collections owing in the sampling probe air is arranged, the residual sample of sampling probe inside and outside wall causes metering inaccurate, though solved measurement problem when using dilution in the sampling probe but dilution can occur can interpenetrate the deficiency that causes change whole blood sample concentration with the sample intersection, the purpose of this invention is to provide a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, another object of the present invention provides a kind of method that is applicable to the sample collection of Automatic Blood Cell Analyzer of using this device.
Technical scheme of the present invention is, a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe, the sampling probe endoporus is connected with the dilution filing provision by pipeline, it is characterized in that: the sampling probe overcoat has sampling probe outer wall washing spare, the waste liquid outlet and the cleaning that are arranged above and below are housed on the sampling probe outer wall washing spare housing sidewall use the dilution import, waste liquid outlet links to each other with waste collecting device, cleans with the dilution import to link to each other with the dilution feedway.
Described waste collecting device comprises waste drains pump and the waste liquid bottle that links to each other with waste liquid outlet.
Described sampling probe is equipped with the sampling probe O-ring seal outward.
Between the syringe pump push rod of described syringe pump and the injection pump housing sealing syringe pump O-ring seal is housed.
Described dilution filing provision comprises syringe pump, and the end of syringe pump has dilution inlet pipe road to be connected with diluting pump, and the inner wall washing solenoid valve is housed between dilution inlet pipe road and the diluting pump, and diluting pump links to each other with the dilution bottle.
The cleaning of described dilution feedway is connected to dilution pipeline one end and cleans with in the dilution import, and the other end links to each other with the dilution bottle with diluting pump through the outer wall washing solenoid valve.
Use the sample collection method of the device of aforesaid a kind of sample collection that is applicable to Automatic Blood Cell Analyzer, it is characterized in that: when drawing whole blood sample, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing spare, the syringe pump push rod is descending, make the upper end of syringe pump push rod be lower than dilution inlet pipe road, the waste drains pump operation produces negative pressure, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing spare, the inner wall washing solenoid valve powers on and opens, the diluting pump operation, dilution flows out from sampling probe is inner, waste liquid outlet at sampling probe outer wall washing spare is pumped, inner wall washing is complete, diluting pump is out of service, the outage of inner wall washing solenoid valve is closed, the syringe pump push rod is up to topmost, descending a bit of again, draw the air of trace, to form air barrier, it is inner that sampling probe goes downwards to the whole blood sample test tube, the whole blood sample that the descending absorption of syringe pump push rod is quantitative, sampling probe is up to the centre position of sampling probe outer wall washing spare, in the up process of sampling probe, the outer wall washing solenoid valve powers on and opens, the diluting pump operation, clean the whole blood sample that adheres on the sampling probe outer wall, clean complete after, diluting pump stops, the outage of outer wall washing solenoid valve is closed, sampling probe carry stop after, the syringe pump push rod is operation upwards, the whole blood sample front end of sampling probe inside is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet of sampling probe outer wall washing spare, waste drains pump is closed, stop to produce negative pressure, the sampling probe translation, the whole blood sample layer is filled into respectively in each different measuring mechanism, after the filling of whole blood sample layer is complete, sampling probe is up to the centre position of sampling probe outer wall washing spare, the waste drains pump operation produces negative pressure, the syringe pump push rod is descending, make the upper end of syringe pump push rod be lower than dilution inlet pipe road, conduct on the inner wall washing solenoid valve, the diluting pump operation, the inner new cleaning fluid that injects of sampling probe is pushed away the whole blood sample end, and the waste liquid outlet of sampled pin outer wall washing spare takes out, and finishes a sample collection filling and sampling probe cleaning process.
The present invention adopts the sampling probe inner space to be full of dilution, before sample drawn, syringe pump extracts first the air of trace, extracts whole blood sample again, the air column that forms is used for isolating dilution and whole blood sample, prevents dilution and whole blood sample permeates mutually.
In order to solve the residual inconsistent problem of whole blood sample, the present invention uses sampling probe outer wall washing spare and dilution that the whole blood sample that the sampling probe outer wall adheres to is cleaned, because existing with dilution, the front end of the whole blood sample of sampling probe inside and terminal sample contact on a small quantity, so before the filling whole blood sample, first the front-end samples of the whole blood sample of sampling probe inside is taken out in sampling probe outer wall washing spare, after the whole blood sample filling was complete, the terminal sample of the whole blood sample of sampling probe inside was taken out in the process of dilution cleaning sampling probe inwall.
Description of drawings
Fig. 1 is structural representation of the present invention,
Fig. 2 is inner each the liquid layer distribution plan of sampling probe.
Among the figure, 1. sampling probe, 2. sampling probe O-ring seal, 3. sampling probe outer wall washing spare, 4. waste drains pump, 5. waste liquid bottle, 6. syringe pump push rod, 7. syringe pump O-ring seal, 8. syringe pump dilution liquid layer, 9. dilution inlet pipe road, 10. diluting pump, 11. dilution bottles, 12. the inner wall washing solenoid valve, 13. outer wall washing solenoid valves, 14. sampling probes dilution liquid layer, 15. air barrier, 16. whole blood sample layers, 17. whole blood sample front ends, 18. whole blood sample is terminal, 19. waste liquid outlets, 20. cleaning dilution imports.
Embodiment
The specific embodiment of the present invention is, as shown in the figure:
Embodiment 1, a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe 1, the sampling probe endoporus is connected with the dilution filing provision by pipeline, it is characterized in that: the sampling probe overcoat has sampling probe outer wall washing spare 3, the waste liquid outlet 19 and the cleaning dilution import 20 that are arranged above and below are housed on the sampling probe outer wall washing spare housing sidewall, and waste liquid outlet links to each other with waste collecting device, cleans with the dilution import to link to each other with the dilution feedway.
Specific operation process is as shown in Figure 1: inner wall washing solenoid valve 12 and outer wall washing solenoid valve 13 are normally close valve, on conduct, the end of syringe pump has dilution inlet pipe road 9 to be connected with diluting pump 10, use syringe pump O-ring seal 7 to connect sealing between syringe pump push rod 6 and the injection pump housing, when syringe pump push rod 6 descending, when making the upper end of syringe pump push rod 6 be lower than dilution inlet pipe road 9, conduct on the inner wall washing solenoid valve 12, diluting pump 10 operations, dilution in the dilution bottle 11 can be filled in the sampling probe 1 by syringe pump inner chamber and associated pipe, use sampling probe O-ring seal 2 to connect sealing between sampling probe 1 and the sampling probe outer wall washing spare 3, the lower end of sampling probe 1 is retracted to the centre position of sampling probe outer wall washing spare 3, waste drains pump 4 operations produce negative pressure, the sampling probe 1 inner cleaning fluid that flows out can be extracted in the waste liquid bottle 5 by waste liquid outlet 19.
When needs are drawn whole blood sample, the lower end of sampling probe 1 is retracted to the centre position of sampling probe outer wall washing spare 3, syringe pump push rod 6 is descending, make the upper end of syringe pump push rod 6 be lower than dilution inlet pipe road 9, waste drains pump 4 operations produce negative pressure, inner wall washing solenoid valve 12 powers on and opens, diluting pump 10 operations, dilution flows out from sampling probe is inner, waste liquid outlet 19 at sampling probe outer wall washing spare 3 is pumped, inner wall washing is complete, diluting pump 10 is out of service, 12 outages of inner wall washing solenoid valve are closed, syringe pump push rod 6 is up to topmost, descending a bit of again, draw the air of trace, to form air barrier 15 as shown in Figure 2, because sampling probe 1 inside is instinct dilution almost, a bit of air barrier 15 is only arranged, so when drawing whole blood sample, the variation of the volume of air of this sub-fraction is very little on the volume impact that gathers whole blood sample, and it is inner that sampling probe 1 goes downwards to the whole blood sample test tube, the quantitative whole blood sample of syringe pump push rod 6 descending absorptions, sampling probe 1 is up to the centre position of sampling probe outer wall washing spare 3, in the sampling probe 1 up process, outer wall washing solenoid valve 13 powers on and opens, diluting pump 10 operations, clean the whole blood sample that adheres on sampling probe 1 outer wall, clean complete after, diluting pump 10 stops, outer wall washing solenoid valve 13 outage is closed.
As shown in Figure 2, between sampling probe dilution liquid layer 14 and the whole blood sample end 18 air barrier 15 is arranged, can well dilution and whole blood sample be separated, avoid interpenetrating, whole blood sample front end 17 is carried to the centre position of outer wall washing spare 3 and is cleaned simultaneously in the outer wall process at sampling probe 1 and contacts with cleaning fluid, cause interpenetrating with cleaning fluid, so sampling probe 1 carry stop after, syringe pump push rod 6 upwards moves a bit of, the whole blood sample front end 17 of sampling probe 1 inside is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet 19 of sampling probe outer wall washing spare 3, waste drains pump is closed, stop to produce negative pressure, sampling probe 1 translation, whole blood sample layer 16 is filled into respectively in each different measuring mechanism, after 16 filling of whole blood sample layer are complete, whole blood sample end 18 is also left in sampling probe 1 inside, sampling probe 1 is up to the centre position of sampling probe outer wall washing spare 3, the waste drains pump operation produces negative pressure, syringe pump push rod 6 is descending, make the upper end of syringe pump push rod 6 be lower than dilution inlet pipe road 9, conduct on the inner wall washing solenoid valve 12, diluting pump 10 operations, the sampling probe 1 inner new cleaning fluid that injects is pushed away whole blood sample end 18, and the waste liquid outlet 19 of sampled pin outer wall washing spare 3 is taken out.
Claims (7)
1. device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe, the sampling probe endoporus is connected with the dilution filing provision by pipeline, it is characterized in that: the sampling probe overcoat has sampling probe outer wall washing spare, the waste liquid outlet and the cleaning dilution import that are arranged above and below are housed on the sampling probe outer wall washing spare housing sidewall, waste liquid outlet links to each other with waste collecting device, cleans with the dilution import to link to each other with the dilution feedway.
2. a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer according to claim 1, it is characterized in that: described waste collecting device comprises waste drains pump and the waste liquid bottle that links to each other with waste liquid outlet.
3. a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer according to claim 1, it is characterized in that: described sampling probe is equipped with the sampling probe O-ring seal outward.
4. a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer according to claim 1 is characterized in that: between the syringe pump push rod of described syringe pump and the injection pump housing sealing syringe pump O-ring seal is housed.
5. a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer according to claim 1, it is characterized in that: described dilution filing provision comprises syringe pump, the end of syringe pump has dilution inlet pipe road to be connected with diluting pump, the inner wall washing solenoid valve is housed between dilution inlet pipe road and the diluting pump, and diluting pump links to each other with the dilution bottle.
6. a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer according to claim 1, it is characterized in that: the cleaning of described dilution feedway is connected to dilution pipeline one end and cleans with in the dilution import, and the other end links to each other with the dilution bottle with diluting pump through the outer wall washing solenoid valve.
7. right to use requires the sample collection method of the device of 1 described a kind of sample collection that is applicable to Automatic Blood Cell Analyzer, it is characterized in that: when drawing whole blood sample, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing spare, the syringe pump push rod is descending, make the upper end of syringe pump push rod be lower than dilution inlet pipe road, the waste drains pump operation produces negative pressure, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing spare, the inner wall washing solenoid valve powers on and opens, the diluting pump operation, dilution flows out from sampling probe is inner, waste liquid outlet at sampling probe outer wall washing spare is pumped, inner wall washing is complete, diluting pump is out of service, the outage of inner wall washing solenoid valve is closed, the syringe pump push rod is up to topmost, descending a bit of again, draw the air of trace, to form air barrier, it is inner that sampling probe goes downwards to the whole blood sample test tube, the whole blood sample that the descending absorption of syringe pump push rod is quantitative, sampling probe is up to the centre position of sampling probe outer wall washing spare, in the up process of sampling probe, the outer wall washing solenoid valve powers on and opens, the diluting pump operation, clean the whole blood sample that adheres on the sampling probe outer wall, clean complete after, diluting pump stops, the outage of outer wall washing solenoid valve is closed, sampling probe carry stop after, the syringe pump push rod is operation upwards, the whole blood sample front end of sampling probe inside is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet of sampling probe outer wall washing spare, waste drains pump is closed, stop to produce negative pressure, the sampling probe translation, the whole blood sample layer is filled into respectively in each different measuring mechanism, after the filling of whole blood sample layer is complete, sampling probe is up to the centre position of sampling probe outer wall washing spare, the waste drains pump operation produces negative pressure, the syringe pump push rod is descending, make the upper end of syringe pump push rod be lower than dilution inlet pipe road, conduct on the inner wall washing solenoid valve, the diluting pump operation, the inner new cleaning fluid that injects of sampling probe is pushed away the whole blood sample end, and the waste liquid outlet of sampled pin outer wall washing spare takes out, and finishes a sample collection filling and sampling probe cleaning process.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210389633.7A CN102901835B (en) | 2012-10-15 | 2012-10-15 | Method and device for collecting sample by full-automatic blood cell analyzer |
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| CN201210389633.7A CN102901835B (en) | 2012-10-15 | 2012-10-15 | Method and device for collecting sample by full-automatic blood cell analyzer |
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| CN102901835A true CN102901835A (en) | 2013-01-30 |
| CN102901835B CN102901835B (en) | 2014-01-29 |
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| CN201210389633.7A Expired - Fee Related CN102901835B (en) | 2012-10-15 | 2012-10-15 | Method and device for collecting sample by full-automatic blood cell analyzer |
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| CN103389265A (en) * | 2013-07-30 | 2013-11-13 | 济南华天恒达科技有限公司 | Sampling and washing system |
| CN105572407A (en) * | 2016-01-27 | 2016-05-11 | 广州万孚生物技术股份有限公司 | Full-automatic fluorescent quantitative immunity analyzer and detection method |
| CN106199032A (en) * | 2016-06-30 | 2016-12-07 | 南京润泽流体控制设备有限公司 | The sampling device of no cross contamination and control method thereof |
| CN106841647A (en) * | 2017-01-25 | 2017-06-13 | 江苏柯伦迪医疗技术有限公司 | Automatic clinical chemistry analyzer and method |
| CN105378478B (en) * | 2014-07-01 | 2017-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of whole blood sample detection method and blood detector |
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| CN103389265A (en) * | 2013-07-30 | 2013-11-13 | 济南华天恒达科技有限公司 | Sampling and washing system |
| CN105378478B (en) * | 2014-07-01 | 2017-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of whole blood sample detection method and blood detector |
| CN105572407A (en) * | 2016-01-27 | 2016-05-11 | 广州万孚生物技术股份有限公司 | Full-automatic fluorescent quantitative immunity analyzer and detection method |
| CN106199032A (en) * | 2016-06-30 | 2016-12-07 | 南京润泽流体控制设备有限公司 | The sampling device of no cross contamination and control method thereof |
| CN106199032B (en) * | 2016-06-30 | 2018-05-08 | 南京润泽流体控制设备有限公司 | A kind of sample injection method of no cross contamination |
| CN107687963B (en) * | 2016-08-04 | 2022-04-26 | 深圳迈瑞生物医疗电子股份有限公司 | Method and device for confirming sample sampling reliability and blood cell analyzer |
| CN107687963A (en) * | 2016-08-04 | 2018-02-13 | 深圳迈瑞生物医疗电子股份有限公司 | Confirmatory sample samples the method, apparatus and blood cell analyzer of reliability |
| CN106841647A (en) * | 2017-01-25 | 2017-06-13 | 江苏柯伦迪医疗技术有限公司 | Automatic clinical chemistry analyzer and method |
| CN107817259A (en) * | 2017-10-30 | 2018-03-20 | 中国水产科学研究院黄海水产研究所 | A kind of method of seawater blood clam section shellfish blood cell collection and Ultrastructural observation |
| CN109959549A (en) * | 2017-12-25 | 2019-07-02 | 深圳迈瑞生物医疗电子股份有限公司 | Sample testing method and sample analyser |
| CN108593407A (en) * | 2018-03-13 | 2018-09-28 | 迈克医疗电子有限公司 | Sample Dilution method and apparatus, Sample analysis instruments and storage medium |
| CN109085019A (en) * | 2018-08-03 | 2018-12-25 | 迪瑞医疗科技股份有限公司 | A kind of sample processing apparatus and method |
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| CN111505313B (en) * | 2020-05-07 | 2022-07-05 | 厦门万渤生物技术有限公司 | Diagnostic kit for combined diagnosis of mycoplasma pneumonia and application thereof |
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| CN111961589B (en) * | 2020-08-12 | 2023-10-31 | 江苏信安佳医疗科技有限公司 | Automatic liquid changing device for three-dimensional organoid sphere culture |
| CN112403953A (en) * | 2020-10-28 | 2021-02-26 | 迈克医疗电子有限公司 | Method and apparatus for cleaning sampling needle assembly |
| CN112403953B (en) * | 2020-10-28 | 2022-04-15 | 迈克医疗电子有限公司 | Method and apparatus for cleaning sampling needle assembly |
| CN114441236A (en) * | 2020-11-03 | 2022-05-06 | 深圳市帝迈生物技术有限公司 | Sampling device and sample analyzer |
| CN112881393A (en) * | 2021-01-22 | 2021-06-01 | 四川沃文特生物技术有限公司 | Liquid suction method in sample detection process |
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