CN106199032A - The sampling device of no cross contamination and control method thereof - Google Patents

The sampling device of no cross contamination and control method thereof Download PDF

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Publication number
CN106199032A
CN106199032A CN201610495365.5A CN201610495365A CN106199032A CN 106199032 A CN106199032 A CN 106199032A CN 201610495365 A CN201610495365 A CN 201610495365A CN 106199032 A CN106199032 A CN 106199032A
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China
Prior art keywords
reagent
container
pipeline
way valve
syringe pump
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Granted
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CN201610495365.5A
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Chinese (zh)
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CN106199032B (en
Inventor
任大鹏
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Nanjing Runze Fluid Controlequipment Co Ltd
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Nanjing Runze Fluid Controlequipment Co Ltd
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Priority to CN201610495365.5A priority Critical patent/CN106199032B/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009Characterised by arrangements for controlling the aspiration or dispense of liquids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004Cleaning sample transfer devices

Abstract

The invention discloses sampling device and the control method thereof of a kind of no cross contamination, described sampling device includes syringe pump, multiple-way valve and soda liquor container, reagent container and waste fluid container: described syringe pump is provided with first mouth of pipe and second mouth of pipe connected with syringe pump cavity volume, first mouth of pipe is connected with soda liquor container by being provided with the first pipeline controlling valve, and second mouth of pipe is connected with the main interface of multiple-way valve by the second pipeline;The stub interface of described multiple-way valve includes an air inlet, a waste liquid outlet, a liquid outlet being connected with external container and some injection ports, and described injection port reagent container connects.Apparatus of the present invention and control method cut off multiple-way valve spool and the solution in reagent cartridge by gas, during may be implemented in sample introduction, avoid spool groove and each injection port retains reagent contact, cause the situation of cross-contamination, the effective accuracy improving analysis of experiments, and the present invention is novel in design, easy to operate, easy to implement, it is simple to promote the use of.

Description

The sampling device of no cross contamination and control method thereof
Technical field
The present invention relates to chemical field, the sampling device of a kind of no cross contamination and control method thereof.
Background technology
Injector is a kind of chemical apparatuses, can insert in analyzing container by testing sample by it, medical medicine analysis, Environment monitoring, inspection and quarantine etc. are widely used in multiple field.In the case of sample to be analyzed or reagent type are many, sample introduction Device generally with manifold (commutation) valve with the use of, but multiple-way valve is during switching inlet, valve core rotation, connection main interface Can have an opportunity to contact with the reagent solution retained in non-targeted branch inlet with the groove of branch inlet, easily make each reagent During shared main interface, produce cross-contamination, have impact on the accuracy of analysis of experiments to a certain extent.
Summary of the invention
The technical purpose of the present invention is to provide sampling device and the control method thereof of a kind of no cross contamination, existing to solve Problem present in technology.
For realizing above-mentioned technical purpose, the technical scheme that the present invention provides is:
The sampling device of a kind of no cross contamination, it is characterised in that include that syringe pump, multiple-way valve and soda liquor container, reagent hold Device and waste fluid container:
Described syringe pump is provided with first mouth of pipe and second mouth of pipe connected with syringe pump cavity volume, and described first mouth of pipe is by being provided with One the first pipeline controlling valve is connected with soda liquor container, and described second mouth of pipe is by the main interface of the second pipeline with multiple-way valve Connect;
Described multiple-way valve is provided around the multiple stub interface being distributed in around main interface, and the plurality of stub interface includes one Air inlet, the waste liquid outlet being connected with waste fluid container, the liquid outlet being connected with external container, remaining stub interface It is injection port, each connects one by one with corresponding reagent container.
On the basis of such scheme, improve further or preferably scheme also include:
Described air inlet is connected with the air inlet pipeline being provided with filter, prevents the impurity in gas from polluting reagent.
Described first controls valve preferably employs two three-way electromagnetic valves.
The port of export of the infusion pipeline connecting liquid outlet and external container is arranged on the bottom of external container;Described outside appearance Device, for sealing container, connects with space outerpace by being arranged on the vent line above external container, described vent line sets There is the second control valve.
The control method of a kind of sampling device for no cross contamination described above, it is characterised in that including:
(A) being full of under cleanout fluid situation in the second pipeline, multiple-way valve switches to air inlet, operation syringe pump suction is a certain amount of Gas is to the step in the second pipeline;
(B), under the situation being full of reagent in connecting the reagent cartridge of injection port and corresponding reagent container, multiple-way valve is switched to This injection port, the second pipeline gas of suction in step (A) is released a certain amount of in this reagent cartridge, is made by operation syringe pump The step cut off by gas between reagent solution and this injection port in this reagent cartridge.
On the basis of above-mentioned control method, further technical scheme also includes:
(C) multiple-way valve switches to the injection port set, and corresponding reagent is aspirated a certain amount of to described second pipe by operation syringe pump Lu Zhong, makes to be full of in the reagent cartridge of this injection port of connection and corresponding reagent container the step of reagent, in the second pipeline reagent with Cleanout fluid is cut off by the gas of suction in step (A);Multiple-way valve switches to waste liquid outlet, and operation syringe pump is managed second Gas in road and reagent enter waste fluid container, make to be full of in the second pipeline the step of cleanout fluid.
Described control method, including preprocessing process, described preprocessing process comprises the following steps:
Open described first and control valve, multiple-way valve is switched to reset position, close all passages of multiple-way valve, operate syringe pump In soda liquor container, aspirate a certain amount of cleanout fluid, close the first control valve afterwards, multiple-way valve is switched to waste liquid and discharges Mouthful, operation syringe pump makes cleanout fluid enter in described second pipeline, and the one end making the second pipeline connect main interface is left necessarily Gas or the gas in the second pipeline perform step (A) after draining;
Circulation performs step (C), step (A), step (B) the most in order, until each bar reagent cartridge connects the one of injection port End is equipped with partition gas.
Described control method, including sample introduction process, described sample introduction process includes:
(D) multiple-way valve switches to set injection port, and operation syringe pump is by should partition gas in the reagent cartridge of injection port Body is pumped in described second pipeline with the reagent of set amount, and multiple-way valve switches to liquid outlet, and operation syringe pump holds to outside Injecting the reagent meeting use demand in device, multiple-way valve switches to waste liquid outlet, operation syringe pump is by the second pipeline The step that the gas of residual or reagent are discharged from waste liquid outlet;
Step (A), step (B) is performed successively afterwards for this injection port.
During sample introduction, make the partition gas volume pushing in reagent pipeline in described step (B) outside more than connecting Container and the infusion pipeline volume of described liquid outlet, in described step (D), when external container injects reagent, by a certain amount of Cut off gas to be together pushed in external container with reagent, it is to avoid reagent remains in infusion pipeline.
When the port of export of the infusion pipeline connecting liquid outlet and external container is arranged on the bottom of external container, described outside Container, for sealing container, connects with space outerpace by being arranged on the vent line above external container, on described vent line It is provided with the second control valve, during described sample introduction, when external container injects reagent, opens described second and control valve, Make air pressure balance;After injection process terminates, it is momentarily turned off described second and controls valve, prevent the reagent in described external container from returning Drop down onto in tube for transfusion.
Apparatus of the present invention and control method cut off multiple-way valve spool and the solution in reagent cartridge by gas, may be implemented in During sample introduction, it is to avoid spool groove with each injection port retain reagent contact, cause the situation of cross-contamination, effectively improve The accuracy of analysis of experiments, and the present invention is novel in design, easy to operate, it is easy to implement, it is simple to promote the use of.
Accompanying drawing explanation
Fig. 1 is the structural representation of one embodiment of the invention;
Fig. 2 is the schematic diagram that in the second pipeline, gas cuts off cleanout fluid and reagent solution.
Detailed description of the invention
In order to be further elucidated with technical scheme and design principle, below in conjunction with the accompanying drawings with specific embodiment to this Invention is described further.
As it is shown in figure 1, the sampling device of a kind of no cross contamination, including syringe pump, multiple-way valve, soda liquor container, reagent The ingredients such as container, waste fluid container, connecting line.
Described syringe pump is double end syringe pump, be provided with liquid reservoir, piston, drive reciprocating motion of the pistons drive mechanism with And for first mouth of pipe of liquid in-out and second mouth of pipe, described first mouth of pipe, second mouth of pipe connect with syringe pump cavity volume, first manages Mouth is connected with soda liquor container by the first pipeline, and described first pipeline is provided with the first control valve of control piper break-make, Preferably employ two three-way electromagnetic valves.Described soda liquor container is loaded with the pure water as cleanout fluid.Described second mouth of pipe is by the Two pipelines are connected with the main interface of multiple-way valve, and in the present embodiment, described multiple-way valve uses eight to lead to valve, and described drive mechanism is advanced Time, syringe pump pushes, and during drive mechanism rollback, syringe pump aspirates.
Described multiple-way valve is provided with main interface and uniform ring around eight stub interface being distributed in around main interface, eight branches Interface include an air inlet, waste liquid outlet being connected with waste fluid container, one be connected with external container go out liquid Mouthful, remaining stub interface is injection port, and the reagent container (reagent 1-reagent 5) the most corresponding with connects.Described waste liquid Outlet, air inlet lay respectively at the left and right sides of liquid outlet.The air inlet pipeline connecting air inlet is provided with air filter, can Avoid the contaminants reagent in air.
In the present embodiment, described first pipeline, the second pipeline, connect the pipeline of container and branch's mouth of pipe, be connected main interface And the internal diameter of the infusion pipeline of external container is between 1mm-2.5mm.
The control method of the sampling device of described no cross contamination, including preprocessing process and sample introduction process:
(1) described preprocessing process is as follows:
First, open described first and control valve, multiple-way valve is switched to the reset position (position between the Ji Liangge branch mouth of pipe Put), close all passages of multiple-way valve, according to flow scheme design, it is clear that operation syringe pump aspirates enough pure water in soda liquor container Washing liquid, closes the first control valve afterwards, and multiple-way valve switches to waste liquid outlet, and operation syringe pump makes the cleaning in its cavity volume Liquid enters in described second pipeline, and the one end connecting main interface at the second pipeline retains a certain amount of gas or in the second pipeline (i.e. there is cleanout fluid to enter in the pipeline that waste liquid outlet connects waste fluid container) after gas drains and perform step (A), described step (A) being to be full of under cleanout fluid situation in the second pipeline, multiple-way valve switches to air inlet, operation syringe pump aspirates a certain amount of gas Body is to the step in the second pipeline.
Secondly, perform step (C), multiple-way valve is switched to a certain injection port, as a example by the injection port of corresponding reagent 1, behaviour Make syringe pump to be aspirated in a certain amount of to described second pipeline by reagent 1, make the reagent cartridge of this injection port of connection and reagent 1 container In be full of reagent 1(gas and be discharged), in the second pipeline, reagent 1 is cut off by the gas of suction in step (A) with cleanout fluid, as Shown in Fig. 2.Multiple-way valve switches to waste liquid outlet afterwards, and operation syringe pump is by the partition gas in the second pipeline and reagent 1 Enter in waste fluid container, make the second pipeline be again filled with cleanout fluid.
Afterwards, then perform step (A);
After again, performing step (B) for reagent 1 injection port, described step (B) is to connect injection port and corresponding reagent container Reagent cartridge is full of under the situation of reagent, multiple-way valve is switched to this injection port, operate syringe pump by the second pipeline in step (A) in, the gas of suction is released a certain amount of in this reagent cartridge, make reagent solution in this reagent cartridge and this injection port it Between the step cut off by gas.
It follows that for reagent 2 to reagent 5, use same operational approach to circulate execution step (C), step in order (A), step (B), until one end that five reagent cartridge connect injection ports is respectively formed partition gas.Then during formal sample introduction, manifold Valve core, in switching rotation process, spool connects the groove of main interface and stub interface with injection port on time, groove with Reagent solution in reagent cartridge does not contacts, and can avoid cross-contamination.
(2) described sample introduction process includes:
Step (D), switches to multiple-way valve set injection port, and as a example by injecting reagent 1 to external container, operation syringe pump is by right Answer cutting off gas and being pumped in described second pipeline more than the reagent 1 of demand in the reagent cartridge of reagent 1 injection port.Will Multiple-way valve switches to liquid outlet, and operation syringe pump injects the reagent meeting use demand in external container, then cut by multiple-way valve Shifting to waste liquid outlet, gas or the reagent of the residual in the second pipeline are discharged by operation syringe pump from waste liquid outlet.
Afterwards, perform step (A), step (B) successively for reagent 1 injection port, make reagent in reagent 1 reagent cartridge with Multiple-way valve spool cuts off yet by gas.
The requirement of root experimental analysis, can be used in the reagent 2 sample introduction process to reagent 5 afterwards by same operational approach In.
In step (D), in the second pipeline aspirate reagent, to external container inject reagent calculate demand time, need calculate Enter and multiple-way valve groove and pipeline inevitably remain the error brought.Or in step (D), according to reagent 1 demand, The reagent 1 of equivalent demand is pumped in the second pipeline by operation syringe pump, when external container injects reagent, by the second pipeline In partition gas and reagent 1 be together pushed in external container, utilize and cut off gas reagent 1 is squeezed out infusion pipeline, keep away Agent of being excused from an examination remains the error brought in infusion pipeline, pushes the partition gas body in reagent pipeline in the most described step (B) Long-pending need to more than connecting the tube for transfusion volume of external container and described liquid outlet, make reagent completely into after in external container, second Cleanout fluid in pipeline need not enter in infusion pipeline.
In above-described embodiment, if the port of export connecting the tube for transfusion of liquid outlet and external container is arranged on the end of external container Portion.Need to be set to described external container seal container, at the blow vent being provided above one and ft connection of external container, described Vent line is provided with the second control valve (not shown).During above-mentioned sample introduction, when external container injects reagent, open Described second controls valve, makes the air pressure balance in external container, and after injection process terminates, need to be momentarily turned off described second control Valve processed, it is to avoid reagent refluxes.
In the present patent application file, " a certain amount of " described in many places also refers not to identical parameter, also refers not to concrete ginseng Number, it will be appreciated that for according to requirements set, and have no effect on and skilled artisan understands that this programme.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.The technology of the industry Personnel, it should be appreciated that the present invention is not restricted to the described embodiments, simply illustrating this described in above-described embodiment and description The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, the present invention Claimed scope is defined by appending claims, description and equivalent thereof.

Claims (10)

1. the sampling device of a no cross contamination, it is characterised in that include syringe pump, multiple-way valve and soda liquor container, reagent Container and waste fluid container:
Described syringe pump is provided with first mouth of pipe and second mouth of pipe connected with syringe pump cavity volume, and described first mouth of pipe is by being provided with One the first pipeline controlling valve is connected with soda liquor container, and described second mouth of pipe is by the main interface of the second pipeline with multiple-way valve Connect;
Described multiple-way valve is provided around the multiple stub interface being distributed in around main interface, and the plurality of stub interface includes one Air inlet, the waste liquid outlet being connected with waste fluid container, the liquid outlet being connected with external container, remaining stub interface It is injection port, each connects one by one with corresponding reagent container.
The sampling device of a kind of no cross contamination the most according to claim 1, it is characterised in that described air inlet be provided with The air inlet pipeline of filter connects.
The sampling device of a kind of no cross contamination the most according to claim 1, it is characterised in that described first controls valve It is two three-way electromagnetic valves.
4. according to the sampling device of a kind of no cross contamination described in claim 1,2 or 3, it is characterised in that connect liquid outlet With the bottom that the port of export of the infusion pipeline of external container is arranged on external container;Described external container, for sealing container, passes through Being arranged on the vent line above external container to connect with space outerpace, described vent line is provided with the second control valve.
5. the control method for the sampling device of no cross contamination as claimed in claim 1, it is characterised in that including:
(A) being full of under cleanout fluid situation in the second pipeline, multiple-way valve switches to air inlet, operation syringe pump suction is a certain amount of Gas is to the step in the second pipeline;
(B), under the situation being full of reagent in connecting the reagent cartridge of injection port and corresponding reagent container, multiple-way valve is switched to This injection port, the second pipeline gas of suction in step (A) is released a certain amount of in this reagent cartridge, is made by operation syringe pump The step cut off by gas between reagent solution and this injection port in this reagent cartridge.
6. the control method for the sampling device of no cross contamination as claimed in claim 5, it is characterised in that including:
(C) multiple-way valve switches to the injection port set, and corresponding reagent is aspirated a certain amount of to described second pipe by operation syringe pump Lu Zhong, makes to be full of in the reagent cartridge of this injection port of connection and corresponding reagent container the step of reagent, in the second pipeline reagent with Cleanout fluid is cut off by the gas of suction in step (A);Multiple-way valve switches to waste liquid outlet, and operation syringe pump is managed second Gas in road and reagent enter waste fluid container, make to be full of in the second pipeline the step of cleanout fluid.
The control method of the sampling device of no cross contamination the most according to claim 6, it is characterised in that include pretreatment Process, described preprocessing process comprises the following steps:
Open described first and control valve, multiple-way valve is switched to reset position, close all passages of multiple-way valve, operate syringe pump In soda liquor container, aspirate a certain amount of cleanout fluid, close the first control valve afterwards, multiple-way valve is switched to waste liquid and discharges Mouthful, operation syringe pump makes cleanout fluid enter in described second pipeline, and the one end making the second pipeline connect main interface is left necessarily Gas or the gas in the second pipeline perform step (A) after draining;
Circulation performs step (C), step (A), step (B) the most in order, until each bar reagent cartridge connects the one of injection port End is equipped with partition gas.
The control method of the sampling device of no cross contamination the most according to claim 7, it is characterised in that include sample introduction mistake Journey, described sample introduction process includes:
(D) multiple-way valve switches to set injection port, and operation syringe pump is by should partition gas in the reagent cartridge of injection port Body is pumped in described second pipeline with the reagent of set amount, and multiple-way valve switches to liquid outlet, and operation syringe pump holds to outside Injecting the reagent meeting use demand in device, multiple-way valve switches to waste liquid outlet, operation syringe pump is by the second pipeline The step that the gas of residual or reagent are discharged from waste liquid outlet;
Step (A), step (B) is performed successively afterwards for this injection port.
The control method of the sampling device of no cross contamination the most according to claim 8, it is characterised in that in sample introduction process In, make the gas volume that cuts off pushing in reagent pipeline in described step (B) be more than connection external container and described liquid outlet Infusion pipeline volume, in described step (D), when external container injects reagent, by a certain amount of partition gas with reagent together It is pushed in external container.
The control method of the sampling device of no cross contamination the most according to claim 8, it is characterised in that connect liquid Mouthful it is arranged on the bottom of external container with the port of export of the infusion pipeline of external container;Described external container is for sealing container, logical Crossing and be arranged on the vent line above external container and connect with space outerpace, described vent line is provided with the second control valve, During described sample introduction, when external container injects reagent, open described second and control valve, after injection process terminates, i.e. Carve and close described second control valve.
CN201610495365.5A 2016-06-30 2016-06-30 A kind of sample injection method of no cross contamination Active CN106199032B (en)

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Cited By (13)

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CN108760420A (en) * 2018-05-08 2018-11-06 湖北工业大学 A kind of sample pre-treatments platform with ultrasonic extraction function
CN108889354A (en) * 2018-07-06 2018-11-27 上海兰博贸易有限公司 A kind of high-precision liquor-transferring system
CN109283138A (en) * 2018-09-30 2019-01-29 宁波万泽微测环境科技股份有限公司 A kind of quantitative sample injection system
CN110531100A (en) * 2019-08-30 2019-12-03 中国航发北京航空材料研究院 A kind of liquid feeding method
CN110658035A (en) * 2019-08-30 2020-01-07 中国航发北京航空材料研究院 Liquid adding method applied to injection pump
CN111239116A (en) * 2020-01-15 2020-06-05 青岛大学附属医院 Reagent kit
CN111373026A (en) * 2017-11-30 2020-07-03 深圳华大智造科技有限公司 Flow path device for gene sequencing, working method thereof and sequencer thereof
CN112742491A (en) * 2019-10-31 2021-05-04 深圳市帝迈生物技术有限公司 Reagent sucking device and reagent sucking method thereof
CN113438974A (en) * 2018-12-28 2021-09-24 阿斯特拉维斯公司 Apparatus and method for treating particle suspensions
CN113567376A (en) * 2021-07-29 2021-10-29 北京京能电力股份有限公司 Device and method for measuring concentration of trace iron ions in water vapor of power plant
CN114088648A (en) * 2021-12-07 2022-02-25 广东盈峰科技有限公司 Gas-liquid dual isolation method for multi-ported valve micro-reagent sampling
CN114433265A (en) * 2020-11-03 2022-05-06 深圳市帝迈生物技术有限公司 Liquid feeding device and sample analyzer
CN114441236A (en) * 2020-11-03 2022-05-06 深圳市帝迈生物技术有限公司 Sampling device and sample analyzer

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CN111373026B (en) * 2017-11-30 2023-06-13 深圳华大智造科技股份有限公司 Flow path device for gene sequencing, working method thereof and sequencer thereof
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CN108760420A (en) * 2018-05-08 2018-11-06 湖北工业大学 A kind of sample pre-treatments platform with ultrasonic extraction function
CN108889354A (en) * 2018-07-06 2018-11-27 上海兰博贸易有限公司 A kind of high-precision liquor-transferring system
CN109283138B (en) * 2018-09-30 2021-08-31 宁波万泽微测环境科技股份有限公司 Quantitative sample introduction system
CN109283138A (en) * 2018-09-30 2019-01-29 宁波万泽微测环境科技股份有限公司 A kind of quantitative sample injection system
CN113438974A (en) * 2018-12-28 2021-09-24 阿斯特拉维斯公司 Apparatus and method for treating particle suspensions
CN110658035A (en) * 2019-08-30 2020-01-07 中国航发北京航空材料研究院 Liquid adding method applied to injection pump
CN110531100A (en) * 2019-08-30 2019-12-03 中国航发北京航空材料研究院 A kind of liquid feeding method
CN112742491A (en) * 2019-10-31 2021-05-04 深圳市帝迈生物技术有限公司 Reagent sucking device and reagent sucking method thereof
CN111239116A (en) * 2020-01-15 2020-06-05 青岛大学附属医院 Reagent kit
CN114433265A (en) * 2020-11-03 2022-05-06 深圳市帝迈生物技术有限公司 Liquid feeding device and sample analyzer
CN114441236A (en) * 2020-11-03 2022-05-06 深圳市帝迈生物技术有限公司 Sampling device and sample analyzer
CN113567376A (en) * 2021-07-29 2021-10-29 北京京能电力股份有限公司 Device and method for measuring concentration of trace iron ions in water vapor of power plant
CN114088648A (en) * 2021-12-07 2022-02-25 广东盈峰科技有限公司 Gas-liquid dual isolation method for multi-ported valve micro-reagent sampling
CN114088648B (en) * 2021-12-07 2024-03-01 广东盈峰科技有限公司 Gas-liquid dual isolation method for sampling micro-reagent of multi-way valve

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Denomination of invention: Cross pollution-free sample introduction method

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