CN108072557B - Constant volume type automatic dilution instrument - Google Patents
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- CN108072557B CN108072557B CN201810173889.1A CN201810173889A CN108072557B CN 108072557 B CN108072557 B CN 108072557B CN 201810173889 A CN201810173889 A CN 201810173889A CN 108072557 B CN108072557 B CN 108072557B
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Abstract
The invention discloses a constant volume type automatic dilution instrument, which comprises a microinjector driven by a stepping motor, a constant injector driven by the stepping motor, a seven-way valve capable of freely switching flow paths and a four-way valve capable of freely switching flow paths; the seven-way valve comprises a first channel for connecting microinjection, a second channel for a to-be-diluted liquid extraction inlet, a third channel for a diluted liquid extraction inlet, a fourth channel for quantitative distribution output, a fifth channel for quantitative distribution input, a sixth channel for connecting with the three-way valve and a seventh channel for micro distribution discharge; the four-way valve comprises a first channel used for connecting a constant injector, a second channel used for extracting an inlet of a liquid to be diluted, a third channel used for connecting a seven-way valve and a fourth channel used for discharging constant dispensing liquid; the micro-injector is connected to the first channel, the constant injector is connected to the first channel, the sixth channel is connected with the three-phase channel, and a small-section pipeline mixer is connected between the sixth channel and the three-phase channel. The invention well solves the defects of various diluting instruments and manual dilution in the past through the linkage of the double-precision injector and the two stepping motors.
Description
Technical Field
The invention relates to a constant volume type automatic dilution instrument which is widely applied to all analysis and test industries and can realize a plurality of advanced functions of high-precision automatic liquid preparation, automatic dilution, simultaneous constant volume dilution, automatic cleaning, auxiliary chemical titration and the like for various liquid samples.
Background
Currently, there are various brands of existing automatic liquid diluters at home and abroad, such as LH-025 automatic diluter of home-made labhands brands, OREM-2000 automatic diluter of labhands brands, electronic bottle neck dispenser of Germany Herschmann (Hirschmann) brands, electronic bottle neck dispenser of Germany Brand brands, electronic bottle neck dispenser of home-made Dalon, and 6D four-in-one automatic liquid sample adding system of Beijing Qing Jishi Si, microLab 600 serial automatic diluter of U.S. Hamilton brands, etc. These automatic diluters are suitable for either inorganic solvents or organic solvents, and can only absorb one liquid at a time, but cannot accurately absorb both the organic solvents and the inorganic solvents; the sampling volume of the electronic bottle mouth liquid dispenser also depends on factors such as ambient temperature, liquid viscosity, liquid property, surface tension and the like, so that the sampling precision is not very high, and the common bottle mouth liquid dispenser can only be used for quantitative liquid dispensing and has no dilution function; from the process of dilution steps, the principles of the automatic diluters are that negative pressure is firstly utilized for precise liquid suction, then liquid is transferred, the dilution efficiency is low, dilution and constant volume cannot be simultaneously carried out, bubbles are easily generated by utilizing negative pressure liquid suction, and the liquid suction volume is easily inaccurate due to the fact that the elastic variable of air is large and redundant air in a liquid suction cavity is also caused. In addition, from the dilution principle, the automatic dilution apparatuses of various brands in the past can achieve automatic precise pipetting and pipetting, but cannot achieve precise volume fixing, because after two different kinds of liquids are mixed, due to the change of the molecular spacing, the total volume inevitably changes more or less, especially when an inorganic liquid is mixed with an organic liquid, the total volume is usually reduced rather than simply adding the volumes of the two liquids, such as 100ml of pure water and 100ml of ethanol, and the volume is obviously less than 200ml (about 194 ml) after the pure water and the 100ml of ethanol are uniformly mixed. Further, the conventional automatic diluter usually has only a single syringe for extracting liquid, the dilution multiple range is small, the single precision is low, the actual use effect is poor, the use frequency is low, and meanwhile, the conventional automatic diluter has two syringes, for example, a micro lab 600 serial automatic diluter of Hamilton brand in united states, which can accurately extract different volumes of liquid, is expensive and cannot be widely used. In summary, various existing automatic or semi-automatic diluters at home and abroad at present cannot realize the functions of precise dilution and constant volume, and cannot replace the work done by manually adopting a volumetric flask in standard inspection, so that a large amount of dilution cannot be adopted, and the manual dilution has the defects of misoperation of test personnel, low dilution efficiency, poor dilution precision, harm to the health of the test personnel due to dilution of toxic and harmful and volatile liquid, waste of redundant reagents or solutions in the preparation process, environmental pollution and the like.
Disclosure of Invention
Aiming at the defects that various automatic or semiautomatic diluters existing at home and abroad at present only can accurately absorb and transfer liquid, cannot accurately dilute and simultaneously fix volume, the extracted liquid is not accurate enough in volume, but manual dilution also has the defects of misoperation of testers, low dilution efficiency, poor dilution precision, harm to the health of the testers due to toxic and harmful liquid in the dilution process and waste and environmental pollution caused by redundant reagents or solutions in the preparation process, the invention relates to the constant volume automatic diluter which can well solve the problems.
The technical scheme adopted by the invention for solving the technical problems is as follows:
A constant volume type automatic dilution instrument is characterized in that: comprises a micro injector driven by a stepping motor, a constant injector driven by the stepping motor, a seven-way valve capable of freely switching flow paths and a four-way valve capable of freely switching flow paths; the seven-way valve comprises a first channel for connecting a micro-injector, a second channel for extracting an inlet of liquid to be diluted, a third channel for extracting the inlet of the diluent, a fourth channel for outputting a quantifying ring, a fifth channel for inputting the quantifying ring, a sixth channel for connecting with a four-way valve and a seventh channel for discharging micro-distribution; the four-way valve comprises a first channel used for connecting a constant injector, a second channel used for a diluent extraction inlet, a third channel used for connecting a seven-way valve and a fourth channel used for constant distribution and discharge; the micro-injector is connected to the first channel, and the constant injector is connected to the first channel; a quantity of rings are connected between the fourth channel and the fifth channel; and the sixth channel is connected with the channels in a three-phase manner, and a small-section pipeline mixer is connected in the middle of the sixth channel.
The seven-way valve and the four-way valve have:
First functional state for micro-dispensing: the first channel is communicated with the second channel for extracting liquid, then the second channel is closed, and the first channel is communicated with the seventh channel for discharging accurate trace liquid;
Second functional state for macrodosing: the second channel is communicated with the first channel and is used for extracting liquid, then the second channel is closed, and the first channel is communicated with the fourth channel and is used for discharging accurate constant liquid;
Third functional state for microdilution: the second channel is communicated with the first channel for extracting the liquid to be diluted, then the second channel is closed, and a certain amount of liquid to be diluted is discharged under the communication of the first channel and the seventh channel; the third channel is communicated with the first channel for extracting the diluent, then the third channel is closed, and a certain amount of diluent is discharged under the communication of the first channel and the seventh channel; finally, uniformly mixing the liquid to be diluted with the diluent;
Fourth functional state for constant dilution: extracting the liquid to be diluted under the condition that the channel II is communicated with the channel I, then closing the channel II, and discharging a certain amount of liquid to be diluted under the condition that the channel I is communicated with the channel IV; extracting the diluent under the conditions that the third channel is communicated with the sixth channel and the third channel is communicated with the first channel, then closing the third channel, and discharging a certain amount of diluent under the conditions that the first channel is communicated with the fourth channel; finally, uniformly mixing the liquid to be diluted with the diluent;
Fifth functional state for high-rate dilution: the second channel is communicated with the first channel and is used for extracting the liquid to be diluted, then the second channel is closed, and the first channel is communicated with the seventh channel and is used for discharging accurate trace liquid to be diluted; extracting the diluent under the conditions that the third channel is communicated with the sixth channel and the third channel is communicated with the first channel, then closing the third channel, and discharging the accurate diluent under the conditions that the first channel is communicated with the fourth channel; finally, uniformly mixing the liquid to be diluted with the diluent;
Sixth functional state for quantitative ring dilution: a section of quantifying ring is connected between the fourth channel and the fifth channel, the liquid to be diluted is extracted from the second channel under the condition that the second channel is communicated with the first channel, then the second channel is closed, the liquid to be diluted is pushed into the quantifying ring under the condition that the first channel is communicated with the fourth channel and the fifth channel is communicated with the seventh channel, the redundant liquid is discharged from the seventh channel, at the moment, the volume of the liquid stored in the quantifying ring is the rated volume of the quantifying ring, then, the first channel and the seventh channel are closed, the third channel is communicated with the fourth channel, the fifth channel is communicated with the sixth channel, the third channel is communicated with the first channel, the diluent is extracted from the third channel, the liquid to be diluted in the quantifying ring enters the constant injector along with the extraction of the diluent, the liquid is diluted and the constant injector at the same time, then the third channel is closed, the first channel is communicated with the fourth channel, and the diluted liquid to be measured is discharged from the fourth channel;
Seventh functional state for dilution while metering: under the condition that the second channel is communicated with the first channel, the microinjector precisely extracts liquid to be diluted from the second channel, the liquid to be diluted is stored in the microinjector, then the second channel is closed, under the condition that the third channel, the sixth channel and the first channel are simultaneously communicated, the sixth channel is simultaneously communicated with the third channel and the first channel is communicated, the constant injector extracts diluent from the third channel, simultaneously the microinjector also pushes the liquid to be diluted into the connecting pipeline, the liquid to be diluted is brought into the pipeline by the diluent, the liquid to be diluted flows through the pipeline mixer to be fully and uniformly mixed, then the constant volume is completed in the constant injector, finally, the third channel is closed, the first channel is communicated with the fourth channel, and the liquid to be measured which is diluted and has the constant volume is discharged from the fourth channel.
The seven-way valve and the four-way valve further have:
Eighth functional state for automatic cleaning: under the condition that the second channel and the third channel are communicated with the first channel at the same time, the micro-injector extracts cleaning liquid from the second channel and the third channel at the same time, then the second channel and the third channel are closed, the first channel is communicated with the seventh channel, and the cleaning liquid is discharged from the seventh channel, so that the micro-injector is cleaned, and the pipeline through which the micro-injector flows is cleaned; and under the conditions that the third channel is communicated with the sixth channel, the sixth channel is communicated with the third channel, the third channel and the second channel are simultaneously communicated with the second channel, the constant injector extracts cleaning liquid from the third channel and the second channel at the same time, then the second channel and the third channel are closed, the first channel is communicated with the fourth channel, and the cleaning liquid is discharged from the fourth channel, so that the constant injector and the pipeline mixer are cleaned together through the pipeline.
Ninth functional state for assisting chemical titration: the method comprises the steps of needing to be combined with a proper electrode, installing a liquid return prevention head at a pipeline outlet of a seventh channel and a pipeline outlet of a fourth channel, controlling two stepping motors, pre-cleaning a micro syringe or a constant syringe, enabling the micro syringe or the constant syringe to be filled with titration liquid and a discharge pipeline, immersing the pipeline outlet of the seventh channel or the pipeline outlet of the fourth channel into a sample solution to be titrated, controlling the stepping motors to drive a piston of the micro syringe or a piston of the constant syringe to precisely move so as to discharge the titration liquid with precise volume, judging the end point of chemical titration according to the reading of the electrode, and calculating the titration result.
The constant volume automatic dilution instrument is mainly composed of a micro glass injector driven by a precise stepping motor, a constant glass injector driven by the precise stepping motor, a seven-way valve capable of freely switching flow paths, a four-way valve capable of freely switching flow paths and a pipeline mixer. Can realize multiple advanced functions such as double-precision liquid preparation, double-precision dilution, simultaneous constant volume dilution, dilution by a quantitative ring, automatic cleaning, auxiliary chemical titration and the like.
The injector piston and the threaded joint of each circulation pipeline are made of corrosion-resistant materials. The circulation pipeline is made of corrosion-resistant flexible materials with certain permeability, can resist various organic reagents and inorganic reagents, can conveniently observe the flowing condition of liquid, the existence of bubbles and the like, and has low replacement cost and convenient replacement. The circulation pipeline can be made of polypropylene (PP) material, or polymethylpentene (PMP) material or copolymer (PFA) material of perfluoropropyl perfluorovinyl ether and polytetrafluoroethylene as required, and has the characteristics of transparency, stronger corrosion resistance and heat resistance, but higher cost. The sealing ring is made of elastic corrosion-resistant materials. The syringe tube wall needs to adopt corrosion-resistant transparent glass material with ultralow expansion coefficient to ensure that the extracted liquid volume is accurate and convenient to observe.
The invention has the advantages of wide application field, high use frequency, high dilution efficiency, wide dilution multiple range, corrosion resistance, pollution resistance, difficult residue, automatic cleaning, and combination with a proper electrode to realize a plurality of advanced functions such as auxiliary chemical titration, etc. Compared with various automatic or semi-automatic diluters in the past, the invention can realize the functions of diluting and simultaneously fixing volume, and has higher dilution precision and dilution efficiency; compared with manual dilution, the method has the advantages of high automation level, simple and quick dilution, totally-enclosed liquid without pollution, effectively shortening the time for preparing the solution, higher dilution precision and dilution efficiency, reducing the consumption of various reagents by the volume of the solution to be tested actually needed in the test, particularly reducing the consumption of various toxic and harmful and volatile reagents, reducing the damage of the test process to the health of human bodies and reducing the pollution of the test to the environment. And thirdly, from the dilution principle, the invention realizes the functions of diluting and fixing volume at the same time in a breakthrough way through the linkage of the program control double-precision stepping motor. In summary, the present invention can well solve various problems of various conventional automatic or semi-automatic diluters and manual dilution.
The beneficial effects of the invention are as follows: the constant volume type automatic diluting instrument can well solve various defects of automatic or semi-automatic diluting instruments and manual dilution in the past through the linkage of a totally-enclosed corrosion-resistant circulating pipeline, two syringes for extracting and distributing liquid in double precision and two stepping motors controlled by a program.
Drawings
Fig. 1 is a schematic structural view of the present invention.
In the figure: 1. microinjector 2, macroinjector 3, seven-way valve 31, first channel 32, second channel 33, third channel 34, fourth channel 35, fifth channel 36, sixth channel 37, seventh channel 4, three-way valve 41, channel one, 42, channel two, 43, channel three, 44, channel four, 5, dosing ring 6, line mixer.
Detailed Description
As shown in FIG. 1, the constant volume automatic dilution apparatus of the present invention comprises a step motor-driven microinjector 1, a step motor-driven macroinjector 2, a seven-way valve 3 capable of freely switching the flow paths, a four-way valve 4 capable of freely switching the flow paths, a dosing ring 5 and a pipeline mixer 6. In this example, a 1000. Mu.L glass syringe was used as the microinjector. A 1000 μl glass syringe was connected at the first channel 31 position of the seven-way valve. The macrosyringe was a 25mL glass syringe. The 25mL glass injector is connected to the first 41 position of the four-way valve, the seven-way valve 3 and the four-way valve 4 are connected in series through a sixth channel 36 and a third channel 43, a small-section pipeline mixer 6 is connected between the sixth channel 36 and the third channel 43, and the liquid to be diluted and the diluent sequentially pass through the seven-way valve and the four-way valve and then become the liquid to be measured.
The passage is described in detail: the first channel 31 is connected with a 1000 mu L micro glass injector, the second channel 32 is a liquid extraction inlet to be diluted, the third channel 33 is a diluent extraction inlet, the fourth channel 34 is a quantitative ring output channel, the fifth channel 35 is a quantitative ring input channel, the sixth channel 36 is a channel connected with a four-way valve, and the seventh channel is a micro liquid dispensing discharge channel; a section of dosing ring 5 is connected between the fifth channel 35 and the sixth channel 36, the fifth channel 35 is a liquid inlet, the sixth channel 36 is a liquid outlet, and dosing rings with different volumes (generally 10 mu L-1000 mu L range) can be used alternatively; the sixth channel 36 is connected to channel three 43; a small section of pipeline mixer is connected between the sixth channel 36 and the third channel 43; channel one 41 was connected to a 25ml constant glass syringe and channel four 44 was the constant dispensing outlet.
Performance: the minimum sampling amount of the microinjector is 10 mu L, the maximum sampling amount is 1000 mu L, and the minimum interval is 1 mu L; the minimum sampling amount of the constant injector is 0.1mL, the maximum is 25mL, and the minimum interval is 0.01mL.
The invention has the following specific functions:
(1) Micro-preparation of liquid: the operation panel sets a dispensing volume, at this time, the second channel 32 is communicated with the first channel 31, the stepper motor drives the microinjector piston to move downwards to extract a certain amount of liquid to be diluted, then the second channel 32 is closed, the first channel 31 is communicated with the seventh channel 37, the stepper motor drives the microinjector piston to move upwards to discharge a certain amount of liquid (if bubbles exist in the extraction pipeline, the action can be repeated for a plurality of times, the liquid to be diluted is extracted after the pipeline is filled with the liquid), the seventh channel 37 is a discharge channel, and both the second channel 32 and the seventh channel 37 can be repeatedly communicated or closed with the first channel 31 for repeatedly cleaning, filling or emptying the injector and the pipeline. The micro-dispensing liquid can be used for preparing 10-1000 mu L of liquid to be measured, or can be used for dispensing more than 1000 mu L by repeatedly operating the piston of the micro-syringe.
(2) Constant liquid preparation: the operation panel sets the dispensing volume, at this time, the second channel 42 is communicated with the first channel 41, the stepping motor drives the constant injector piston to move downwards to extract a certain amount of liquid, then the second channel 42 is closed, the first channel 41 is communicated with the fourth channel 44, and the stepping motor drives the constant injector piston to move upwards to discharge a certain amount of liquid (if bubbles exist in the extraction pipeline, the action can be repeated for a plurality of times, so that the pipeline is filled with the liquid to be diluted and then the liquid is extracted). Channel four 44 is a vent channel, and both channel two 42 and channel four 44 can be repeatedly connected to and disconnected from channel one 41 for repeated purging, filling or draining of syringes and tubing. The constant dispensing liquid can be used for preparing 0.1-25 mL of liquid to be tested, and the piston of the constant syringe can be repeatedly operated for more than 25mL of liquid to be tested.
(3) Microdilution (when the volume of liquid to be measured is small): the first step is to extract the liquid to be diluted, the first channel 32 is communicated with the first channel 31, the step motor drives the microinjector piston to move downwards to extract a certain amount of the liquid to be diluted from the second channel 32, then the second channel 32 is closed, the step motor drives the microinjector piston to move upwards to push out a precise amount of the liquid to be diluted from the seventh channel 37 to be injected into a container (if bubbles exist in an extraction pipeline, the action can be repeated for a plurality of times, so that the liquid to be diluted is filled in the pipeline and then extracted); the second step is to extract the diluent, at this time, the third channel 33 is communicated with the first channel 31, the stepper motor drives the microinjector piston to move downwards to extract a certain amount of diluent from the third channel 33, then the third channel 33 is closed, the stepper motor drives the microinjector piston to move upwards to push out a precise amount of diluent from the seventh channel 37 to be injected into the same container (if bubbles exist in the extraction pipeline, the action can be repeated for a plurality of times, so that the pipeline is filled with the diluent and then the diluent is extracted). And finally, uniformly mixing the liquid to be diluted and the diluent in the container to obtain the liquid to be measured. The micro dilution can generally realize any integer multiple dilution of 1-100 times, and can also enable the piston of the micro injector to reciprocate for multiple times, so as to extract more diluent, and the micro dilution can be realized by 1000 times when the micro dilution is used for micro dilution of more than 100 times, such as 10 times.
(4) Constant dilution (when the volume of liquid to be measured is large): the first step is to extract the liquid to be diluted, the channel two 42 is communicated with the channel one 41, the stepping motor drives the constant injector piston to move downwards to extract a certain amount of the liquid to be diluted from the channel two 42, then the channel two 42 is closed, the channel one 41 is communicated with the channel four 44, the stepping motor drives the constant injector piston to move upwards to discharge a precise amount of the liquid to be diluted from the channel four 44, and the liquid to be diluted is injected into a container (if bubbles exist in an extraction pipeline, the action can be repeated for a plurality of times, so that the pipeline is filled with the liquid to be diluted and then the liquid to be diluted is extracted again); the second step is to extract the diluent, at this time, the third channel 33 is communicated with the sixth channel 36, the third channel 43 is communicated with the first channel 41, the stepper motor drives the constant injector piston to move downwards to extract a certain amount of diluent from the third channel 33, then the third channel 43 is closed, the stepper motor drives the constant injector piston to move upwards to discharge a certain amount of diluent from the fourth channel 44 to be injected into the same container (if bubbles exist in the extraction pipeline, the action can be repeated for a plurality of times, so that the diluent is filled in the pipeline and then extracted). And finally, uniformly mixing the liquid to be diluted in the container with the diluent to obtain the liquid to be measured. The constant dilution can generally realize any integer multiple dilution of 1-250 times, and can also enable the piston of the constant injector to reciprocate for multiple times, so as to extract more diluent, and the constant dilution can be realized by more than 250 times, such as 10 times of reciprocation.
(5) High-rate dilution: the first step is to extract the liquid to be diluted, the second channel 32 is communicated with the first channel 31, the step motor drives the microinjector piston to move downwards to extract a certain amount of the liquid to be diluted from the second channel 32, then the second channel 32 is closed, the step motor drives the microinjector piston to move upwards to discharge the accurate and quantitative liquid to be diluted from the seventh channel 37 and inject the accurate and quantitative liquid to be diluted into a container (if bubbles exist in an extraction pipeline, the action can be repeated for a plurality of times, so that the liquid to be diluted is filled in the pipeline and then extracted); the second step is to extract the diluent, the third channel 33 is communicated with the sixth channel 36, the third channel 43 is communicated with the first channel 41, the stepper motor drives the constant injector piston to move downwards to extract a certain amount of diluent from the third channel 33, then the third channel 43 is closed, the stepper motor drives the constant injector piston to move upwards to discharge a certain amount of diluent from the fourth channel 44 to be injected into the same container (if bubbles exist in the extraction pipeline, the action can be repeated for a plurality of times, so that the pipeline is filled with the diluent and then the diluent is extracted). And finally, uniformly mixing the liquid to be diluted and the diluent in the container to obtain the liquid to be measured. The high dilution ratio mode can generally realize any integer multiple dilution of 1-2500 times, and can also enable the piston of the constant injector to reciprocate for multiple times, so as to extract more diluent, and can realize 25000 times dilution when used for high dilution ratio of more than 2500 times, such as 10 times reciprocation.
(6) Quantitative ring dilution: depending on the requirements of the test, different volumes of dosing rings may be connected between the fourth channel 34 and the fifth channel 35, typically varying in volume from 10 μl to 1000 μl. When the liquid to be diluted is extracted, the second channel 32 is communicated with the first channel 31, the step motor drives the micro-injector piston to move downwards to extract a certain amount of liquid to be diluted from the second channel 32, then the second channel 32 is closed, the first channel 31 is communicated with the fourth channel 34, the fifth channel 35 is communicated with the seventh channel 37, the step motor drives the micro-injector piston to move upwards to push the liquid to be diluted into the dosing ring, the surplus liquid is discharged from the seventh channel 37, the volume of the liquid stored in the dosing ring is the volume of the dosing ring, then the first channel 31 is closed, the third channel 33 is communicated with the fourth channel 34, the fifth channel 35 is communicated with the sixth channel 36, meanwhile, the third channel 43 is communicated with the first channel 41, the step motor drives the constant injector 4 to move downwards, the liquid to be diluted in the dosing ring is simultaneously brought into the injector 4, the constant volume of the liquid to be diluted is fully mixed in the injector 4 when the liquid to be diluted is fully mixed with the diluent liquid through the pipe mixer 6, and finally the third channel 33 is closed, the constant channel 43 is well communicated with the fourth channel 44, and the constant volume of the liquid to be diluted is discharged from the fourth channel 44.
(7) Diluting and simultaneously fixing volume: the device is suitable for mixing two liquids with different properties, has accurate volume after volume fixing, high dilution precision and high dilution efficiency, and can replace manual volumetric flask operation. The second channel 32 is communicated with the first channel 31, the step motor drives the micro-injector piston to move downwards to extract a certain amount of liquid to be diluted from the second channel 32, then the second channel 32 is closed, the third channel 33, the sixth channel 36 and the first channel 31 are communicated with each other, the third channel 43 is communicated with the first channel 41, then the micro-injector 3 piston moves upwards while the constant injector 4 piston moves downwards, the diluent is extracted from the third channel 33 to drive the liquid to be diluted into the constant injector 4 (the liquid to be diluted and the diluent flow are fully mixed when passing through the pipeline mixer 6), the liquid to be diluted and the diluent are diluted in the constant injector 4 while the constant injector is fixed in volume, then the third channel 43 is closed, the first channel 41 is communicated with the fourth channel 44, and the step motor drives the constant injector 4 piston to move upwards to discharge the liquid to be measured diluted while the constant injector 4 is fixed in volume. The constant volume dilution can be realized by 1-2500 times of arbitrary integer multiple dilution, or the constant injector piston can be reciprocated for multiple times to extract more diluent, and the diluent can be used for 2500 times of dilution, such as 10 times of reciprocation, and 25000 times of dilution can be realized.
(8) And (3) automatic cleaning: the micro-injector 3 simultaneously extracts the cleaning fluid from the second channel 32 and the third channel 33 under the condition that the second channel 32 and the third channel 33 are simultaneously communicated with the first channel 31, then the second channel 32 and the third channel 33 are closed, the first channel 31 is communicated with the seventh channel 37, the cleaning fluid is discharged from the seventh channel 37, and the pipeline through which the micro-injector flows is simultaneously cleaned; with the third channel 31 in communication with the sixth channel 36 and the third and second channels 43, 42 in simultaneous communication with the first channel 41, the constant injector 4 simultaneously extracts the cleaning fluid from the third and second channels 33, 42, and then both channels 42, 43 are closed, the first channel 41 in communication with the fourth channel 44, and the cleaning fluid is discharged from the fourth channel 44, which is used to clean the constant injector and the line mixer 6, and the line through which the cleaning fluid flows is cleaned together, which may be repeated a plurality of times.
(9) Auxiliary chemical titration: the method comprises the steps of needing to be combined with a proper electrode, installing liquid return-preventing heads at pipeline discharge ports of a seventh channel 37 and a fourth channel 44, controlling two stepping motors, pre-cleaning the microinjector 3 or the constant injector 4, filling the microinjector 3 or the constant injector 4 with a titration liquid and a discharge pipeline, immersing the pipeline discharge ports of the seventh channel 37 or the fourth channel 44 into a sample solution to be titrated, controlling the stepping motors to drive a piston of the microinjector 3 or a piston of the constant injector 4 to precisely move so as to discharge the titration liquid with precise volume, judging the end point of chemical titration according to the reading of the electrode, and calculating the titration result.
Claims (5)
1. A constant volume type automatic dilution instrument is characterized in that: comprises a micro injector driven by a stepping motor, a constant injector driven by the stepping motor, a seven-way valve capable of freely switching flow paths and a four-way valve capable of freely switching flow paths; the seven-way valve comprises a first channel used for connecting a micro-injector, a second channel used for an extracting inlet of a liquid to be diluted, a third channel used for an extracting inlet of the liquid to be diluted, a fourth channel used for outputting a quantitative ring, a fifth channel used for inputting the quantitative ring, a sixth channel used for connecting a four-way valve and a seventh channel used for micro-distribution and discharge; the four-way valve comprises a first channel used for connecting a constant injector, a second channel used for extracting an inlet of liquid to be diluted, a third channel used for connecting a seven-way valve and a fourth channel used for discharging constant liquid; the micro-injector is connected to the first channel, and the constant injector is connected to the first channel; a quantity of rings are connected between the fourth channel and the fifth channel; the sixth channel is connected with the three phases of the channels through a pipeline mixer;
The seven-way valve and the four-way valve have:
First functional state for micro-dispensing: the first channel is communicated with the second channel for extracting liquid, then the second channel is closed, and the first channel is communicated with the seventh channel for discharging accurate trace liquid;
Second functional state for macrodosing: the second channel is communicated with the first channel and is used for extracting liquid, then the second channel is closed, and the first channel is communicated with the fourth channel and is used for discharging accurate constant liquid;
Third functional state for microdilution: the second channel is communicated with the first channel for extracting the liquid to be diluted, then the second channel is closed, and a certain amount of liquid to be diluted is discharged under the communication of the first channel and the seventh channel; extracting the diluent under the condition that the third channel is communicated with the first channel, closing the third channel, and discharging a certain amount of diluent under the condition that the first channel is communicated with the seventh channel; finally, uniformly mixing the liquid to be diluted with the diluent;
fourth functional state for constant dilution: extracting the liquid to be diluted under the condition that the channel II is communicated with the channel I, closing the channel II, and discharging a certain amount of liquid to be diluted under the condition that the channel I is communicated with the channel IV; extracting the diluent under the conditions that the third channel is communicated with the sixth channel, the third channel is communicated with the first channel, then the third channel is closed, and a certain amount of diluent is discharged under the conditions that the first channel is communicated with the fourth channel; finally, uniformly mixing the liquid to be diluted with the diluent;
Fifth functional state for high-rate dilution: the second channel is communicated with the first channel and is used for extracting the liquid to be diluted, then the second channel is closed, and the first channel is communicated with the seventh channel and is used for discharging accurate trace liquid to be diluted; extracting the diluent under the conditions that the third channel is communicated with the sixth channel and the third channel is communicated with the first channel, then closing the third channel, and discharging the accurate diluent under the conditions that the first channel is communicated with the fourth channel; finally, uniformly mixing the liquid to be diluted with the diluent;
Sixth functional state for quantitative ring dilution: a section of quantifying ring is connected between the fourth channel and the fifth channel, the liquid to be diluted is extracted from the second channel under the condition that the second channel is communicated with the first channel, then the second channel is closed, the liquid to be diluted is pushed into the quantifying ring under the condition that the first channel is communicated with the fourth channel and the fifth channel is communicated with the seventh channel, the redundant liquid is discharged from the seventh channel, at the moment, the volume of the liquid stored in the quantifying ring is the rated volume of the quantifying ring, then, the first channel and the seventh channel are closed, the third channel is communicated with the fourth channel, the fifth channel is communicated with the sixth channel, the third channel is communicated with the first channel, the diluent is extracted from the third channel, the liquid to be diluted in the quantifying ring enters the constant injector along with the extraction of the diluent, the liquid is diluted and the constant injector at the same time, then the third channel is closed, the first channel is communicated with the fourth channel, and the diluted liquid to be measured is discharged from the fourth channel;
Seventh functional state for dilution while metering: under the condition that the second channel is communicated with the first channel, the microinjector precisely extracts liquid to be diluted from the second channel, the liquid to be diluted is stored in the microinjector, then the second channel is closed, under the condition that the third channel, the sixth channel and the first channel are simultaneously communicated, the sixth channel is simultaneously communicated with the third channel and the first channel is communicated, the constant injector extracts diluent from the third channel, simultaneously the microinjector also pushes the liquid to be diluted into the connecting pipeline, the liquid to be diluted is brought into the pipeline by the diluent, the liquid to be diluted flows through the pipeline mixer to be fully and uniformly mixed, then the constant volume is completed in the constant injector, finally, the third channel is closed, the first channel is communicated with the fourth channel, and the liquid to be measured which is diluted and has the constant volume is discharged from the fourth channel.
2. The constant volume automatic dilution apparatus according to claim 1, wherein: the seven-way valve and the four-way valve further have:
Eighth functional state for automatic cleaning: under the condition that the second channel and the third channel are communicated with the first channel at the same time, the micro-injector extracts cleaning liquid from the second channel and the third channel at the same time, then the second channel and the third channel are closed, the first channel is communicated with the seventh channel, and the cleaning liquid is discharged from the seventh channel, so that the micro-injector is cleaned, and the pipeline through which the micro-injector flows is cleaned; under the conditions that the third channel is communicated with the sixth channel, the sixth channel is communicated with the third channel, the third channel and the second channel are simultaneously communicated with the second channel, the constant injector extracts cleaning liquid from the third channel and the second channel at the same time, then the second channel and the third channel are closed, the first channel is communicated with the fourth channel, and the cleaning liquid is discharged from the fourth channel, so that the constant injector and the pipeline mixer are cleaned together through the pipeline;
Ninth functional state for assisting chemical titration: the method comprises the steps of needing to be combined with a proper electrode, installing a liquid return prevention head at a pipeline outlet of a seventh channel and a pipeline outlet of a fourth channel, controlling two stepping motors, pre-cleaning a micro syringe or a constant syringe, enabling the micro syringe or the constant syringe to be filled with titration liquid and a discharge pipeline, immersing the pipeline outlet of the seventh channel or the pipeline outlet of the fourth channel into a sample solution to be titrated, controlling the stepping motors to drive a piston of the micro syringe or a piston of the constant syringe to precisely move so as to discharge the titration liquid with precise volume, judging the end point of chemical titration according to the reading of the electrode, and calculating the titration result.
3. The constant volume automatic dilution apparatus according to claim 2, wherein: the microinjector and the macroinjector are transparent glass injectors.
4. A constant volume automatic dilution apparatus according to claim 3, wherein: the microinjector was 1000 μl.
5. A constant volume automatic dilution apparatus according to claim 3, wherein: the constant syringe was 25mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810173889.1A CN108072557B (en) | 2018-03-02 | Constant volume type automatic dilution instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810173889.1A CN108072557B (en) | 2018-03-02 | Constant volume type automatic dilution instrument |
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| Publication Number | Publication Date |
|---|---|
| CN108072557A CN108072557A (en) | 2018-05-25 |
| CN108072557B true CN108072557B (en) | 2024-06-11 |
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