CN208026537U - A kind of volum-controlled type automatic dilutor - Google Patents
A kind of volum-controlled type automatic dilutor Download PDFInfo
- Publication number
- CN208026537U CN208026537U CN201820292516.1U CN201820292516U CN208026537U CN 208026537 U CN208026537 U CN 208026537U CN 201820292516 U CN201820292516 U CN 201820292516U CN 208026537 U CN208026537 U CN 208026537U
- Authority
- CN
- China
- Prior art keywords
- channel
- liquid
- diluted
- syringe
- constant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
- 239000007788 liquid Substances 0.000 claims abstract description 140
- 239000012895 dilution Substances 0.000 claims abstract description 102
- 238000010790 dilution Methods 0.000 claims abstract description 102
- 239000000284 extract Substances 0.000 claims abstract description 25
- 238000009826 distribution Methods 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims description 29
- 238000000605 extraction Methods 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 23
- 238000012360 testing method Methods 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 10
- 238000004448 titration Methods 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000012488 sample solution Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 2
- 238000000520 microinjection Methods 0.000 abstract 1
- 230000009471 action Effects 0.000 description 9
- 230000006870 function Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 230000007797 corrosion Effects 0.000 description 6
- 238000005260 corrosion Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000005213 imbibition Methods 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 230000007096 poisonous effect Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010034703 Perseveration Diseases 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 239000003049 inorganic solvent Substances 0.000 description 2
- 229910001867 inorganic solvent Inorganic materials 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229920000306 polymethylpentene Polymers 0.000 description 2
- 239000011116 polymethylpentene Substances 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 101100008048 Caenorhabditis elegans cut-4 gene Proteins 0.000 description 1
- QYKIQEUNHZKYBP-UHFFFAOYSA-N Vinyl ether Chemical compound C=COC=C QYKIQEUNHZKYBP-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 125000005010 perfluoroalkyl group Chemical group 0.000 description 1
- 125000005009 perfluoropropyl group Chemical group FC(C(C(F)(F)F)(F)F)(F)* 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of volum-controlled type automatic dilutor, including a stepper motor driven micro syringe, a stepper motor driven constant syringe, one can free switching flow path seven port valves and one can free switching flow path four-way valve；Seven port valves include second channel for connecting the first passage of micro-injection, extracting entrance for liquid to be diluted, extract the third channel of entrance for dilution, for the fourth lane of rationed output, for the Five-channel of rationed input, the 6th channel for being connect with triple valve and the 7th channel for micro distribution discharge；Four-way valve includes the channel two for connecting the channel one of constant syringe, extracting entrance for liquid to be diluted, the channel three for being connect with seven port valves, matches the channel four that liquid is discharged for constant.The utility model well solves previous various dilution instrument and manually diluted shortcomings by the linkage of double precision syringe, two stepper motors.
Description
Technical field
The utility model is related to a kind of volum-controlled type automatic dilutor, be widely used in all analyses test industries, it can be achieved that
Plurality of liquid sample is carried out High Precision Automatic with liquid, automatic dilution, dilution while constant volume, automatic cleaning and assistant chemical drop
Many advanced functions such as fixed.
Background technology
Currently, existing liquid automatic dilutor brand is numerous both at home and abroad, such as LH-025 types of domestic labhands brands
Automatic dilutor, the OREM-2000 type automatic dilutors of labhands brands, German He Shiman（Hirschmann）The electricity of brand
Sub- bottleneck dispenser, German Brand brands electronics bottleneck dispenser, domestic big dragon electronics bottleneck dispenser, in addition, also domestic
600 series of MicroLab of the 6D four-in-one automated fluid sample adding systems and U.S.'s Hamilton brands of thinking wound is ground clearly in Beijing
Automatic dilutor dilution instrument etc..These automatic dilutors are only applicable to inorganic solvent, or are only applicable to organic solvent, and one
It is secondary to draw a kind of liquid, accurate simultaneously it can not draw organic solvent and inorganic solvent；And the sampling of electronics bottleneck dispenser
Volume additionally depends on the factors such as environment temperature, liquid viscosity, liquid property, surface tension, lead to sampling precision also and be not it is very high,
And common bottleneck dispenser is only used for quantitative liquid separating, does not have dilution function；In terms of dilution step process, these are automatic dilute
The principle for releasing instrument is all the then liquid relief first with negative pressure precision imbibition, and dilution efficiency is low, and dilution and constant volume cannot be carried out at the same time,
And be easy to generate bubble using negative-pressure liquid suction, since the elastic module of air is big, extra air is also easy to lead in imbibition chamber
Cause the inaccuracy of imbibition volume.In addition, from dilution principle, the automatic dilutor of previous various brands can be accomplished automatically
Accurate imbibition and liquid relief can not but accomplish accurate constant volume, and reason is after two different kinds of liquid mixing, due to molecule
Spacing changes, and more or less variation can inevitably occur for total volume, especially when a kind of inorganic liquid and one kind
After organic liquid mixing, the simple addition that total volume would generally reduce without being two kinds of liquid volumes, for example 100ml pure water
Volume is significantly less than 200ml after mixing with 100ml ethyl alcohol（About 194ml）.Again, previous automatic dilutor is usual only
Be used for extracting liq with single syringe, extension rate range is small, single precision, practical effect is bad, frequency of use not
Height, and have simultaneously there are two syringe, the double precision automatic dilutor of the liquid of accurate extraction different volumes can be distinguished, for example
The serial automatic dilutors of MicroLab 600 of U.S.'s Hamilton brands, then it is expensive, it can not largely popularize.To sum up institute
It states, existing various automatically or semi-automatically dilution instrument can not achieve the accurate function of diluting constant volume simultaneously both at home and abroad at present, can not
The work that substitute specification is manually completed using volumetric flask in examining, thus can not largely use, and diluted by hand in the presence of experiment
Personnel's maloperation, dilution efficiency is low, dilution low precision, dilutes poisonous and harmful and effumability liquid hazard test personnel be good for
Extra reagent or solution cause the shortcomings such as waste, pollution environment in health, process for preparation.
Invention content
For at present both at home and abroad existing various automatically or semi-automatically dilution instrument generally existings can only accurate imbibition and shifting
Liquid, cannot accurate dilution constant volume simultaneously, the liquid volume of extraction not enough precisely dilutes that there are testing crew accidentally behaviour by hand
Make, dilution efficiency is low, dilution low precision, poisonous and harmful and effumability liquid hazard test personnel health in dilution,
Extra reagent or solution cause waste, the pollution shortcomings such as environment in process for preparation, the utility model is related to one kind it is fixed
Appearance type automatic dilutor can well solve the above problem.
Technical solution is used by the utility model solves above-mentioned technical problem：
A kind of volum-controlled type automatic dilutor, it is characterised in that：Including a stepper motor driven micro syringe, one
Stepper motor driven constant syringe, one can free switching flow path seven port valves and one can free switching flow path four-way
Valve；Seven port valve includes for connecting the first passage of micro syringe, leading to for the second of liquid extraction entrance to be diluted
Road, for dilution extract entrance third channel, for quantitative loop output fourth lane, for quantitative loop input the 5th
Channel, the 6th channel for being connect with four-way valve and the 7th channel for micro distribution discharge；The four-way valve includes
For connecting the channel one of constant syringe, extracting channel two, the channel for being connect with seven port valves of entrance for dilution
Three, the channel four for constant distribution discharge；The micro syringe is connected on the first passage, the constant syringe
It is connected on the channel one；It is connected with a certain amount of ring between the fourth lane and Five-channel；6th channel and
Channel three is connected, centre is connected with a bit of line mixer.
Seven port valve and four-way valve have：
For micro the first functional status with liquid：Extracting liq is used in the case where first passage is connected with the second channel, with
Second channel is closed afterwards, and accurately micro liquid is discharged in the case where first passage is connected to the 7th channel；
Match the second functional status of liquid for constant：Extracting liq is used in the case where channel two is connected to channel one, it is then logical
Road two is closed, and accurately constant liquid is discharged in the case where channel one is connected to channel four；
Third functional status for Microdilution：It is connected under second channel and first passage for extracting liquid to be diluted
Body, subsequent second channel are closed, and a certain amount of liquid to be diluted is discharged in the case where first passage is connected to the 7th channel；It is logical in third
It is connected under road and first passage for extracting dilution, subsequent third channel is closed, in the case where first passage is connected to the 7th channel
A certain amount of dilution is discharged；Finally, by liquid to be diluted and dilution mixing；
For diluted 4th functional status of constant：Liquid to be diluted is extracted in the case where channel two is connected to channel one, then
Channel two is closed, and channel one is connected to a certain amount of liquid to be diluted of discharge with channel four；Be connected to the 6th channel in third channel,
Channel three is connected to down extraction dilution with channel one, and then channel three is closed, and is discharged in the case where channel one is connected to channel four certain
The dilution of amount；Finally, by liquid to be diluted and dilution mixing；
For the diluted five-function state of high magnification：It is to be diluted for extracting in the case where second channel is connected to first passage
Liquid, subsequent second channel are closed, and accurately micro liquid to be diluted is discharged in the case where first passage is connected to the 7th channel；?
Triple channel is connected to the 6th channel, channel three be connected to down with channel one extraction dilution, subsequent channel three close, channel one with
The connection of channel four is lower to be discharged accurately dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 6th functional status of quantitative loop：One section of quantitative loop is connected between fourth lane and Five-channel,
Liquid to be diluted is extracted from second channel in the case where second channel is connected to first passage, subsequent second channel is closed, logical first
Road is connected to fourth lane, Five-channel is connected to down with the 7th channel simultaneously, liquid to be diluted is pushed into quantitative loop, extra liquid
Body is discharged by the 7th channel, and the volume of the liquid stored in quantitative loop at this time is exactly the nominal volume of the quantitative loop, then, first
Channel is turned off with the 7th channel, and third channel is connected to fourth lane, while Five-channel is connected to the 6th channel, and channel
Three are connected to channel one, extract dilution from third channel, the liquid to be diluted in quantitative loop can be with the extraction one of dilution
And enter constant syringe, so that liquid is completed at the same time dilution and constant volume in constant syringe, subsequent channel three is closed, channel one
It is connected to channel four, the testing liquid diluted is discharged from channel four；
The 7th functional status for diluting constant volume simultaneously：In the case where second channel is connected to first passage, micro syringe
Liquid to be diluted is extracted from second channel precision, fluid storage to be diluted in micro syringe, close by subsequent second channel,
Third channel, the 6th channel, first passage is connected to simultaneously, the 6th channel is connected to down with channel tee joint, channel three and channel one,
From third channel extraction dilution, liquid to be diluted is also pushed into connecting line constant syringe by micro syringe simultaneously, is waited for dilute
It releases liquid and is diluted liquid band and enter in pipeline, flow through line mixer and mixed well, it is fixed then to be completed in constant syringe
Hold, finally, channel three is closed, and channel one is connected to channel four, from the discharge dilution of channel four while the good testing liquid of constant volume.
Seven port valve and four-way valve also have：
The 8th functional status for cleaning automatically：It is micro- in the case where second channel, third channel are connected to simultaneously with first passage
Amount syringe extracts cleaning solution simultaneously from second channel, third channel, and subsequent second channel, third channel are turned off, and first is logical
Road is connected to the 7th channel, and cleaning solution is discharged from the 7th channel, and, for cleaning micro syringe, the pipeline flowed through obtains together for this
To cleaning；Be connected to the 6th channel in third channel, the 6th channel and channel tee joint, channel three and channel two again with channel one
Under being connected to simultaneously, constant syringe extracts cleaning solution simultaneously from third channel and channel two, and subsequent channel two, channel three are closed
It closes, channel one is connected to channel four, cleaning solution is discharged from channel four, this is for cleaning constant syringe and line mixer, institute
The pipeline flowed through is cleaned together.
The 9th functional status for assistant chemical titration：It needs to be used in combination with electrode appropriate, the 7th channel and logical
The tubes rows exit in road four needs installation liquid only to return head, then controls two stepper motors, first prerinse micro syringe
Or constant syringe, make to be full of titrating solution in micro syringe or constant syringe and in discharge line, then lead to the 7th
The pipeline outlet in road or channel four immerses in sample solution to be titrated, controls respective stepper motor driving micro syringe
Piston or constant syringe piston carry out accurate movement the titrating solution of accurate volume is discharged, according to the reading judgementization of electrode
The terminal for learning titration, calculates the result of titration.
The utility model volum-controlled type automatic dilutor mainly by a precision stepper motor driving micro glass syringe,
One precision stepper motor driving constant glass syringe, one can free switching flow path seven port valves, one can freely cut
The four-way valve on change of current road, a line mixer are constituted.May be implemented double precision with liquid, double precision dilution, dilution simultaneously constant volume,
Be diluted with quantitative loop, automatically cleaning, assistant chemical titration etc. a variety of advanced functions.
The nipple of the syringe piston of the utility model and each flow pipe is all made of resistant material and is made.Circulation
Pipeline need to be used corrosion-resistant and is made with certain permeability, flexible material, can both be resistant to a variety of organic reagents and
Inorganic reagent can easily observe the mobility status of liquid again, have a situations such as bubble-free, and the alternative costs of flow pipe need to be compared with
It is low and facilitate replacement.Polypropylene can be used in flow pipe（PP）Material can also use polymethylpentene as needed（PMP）Material
Or the copolymer of perfluoro propyl perfluoroalkyl vinyl ether and polytetrafluoroethylene (PTFE)（PFA）Material equally has transparent characteristic, and corrosion resistant
Corrosion, heat resistance are stronger, but cost is higher.Sealing ring is using elastic resistant material.Injector wall need to use it is corrosion-resistant and
Transparent glass material with super-low expansion coefficient, to ensure that the liquid volume of extraction is accurate, facilitate observation.
The applicable field of the utility model is wide, frequency of use is high, dilution efficiency is high, extension rate range is wide, corrosion-resistant, anti-
It pollutes, is not easy to remain, can clean automatically, moreover it is possible to be used in combination with suitable electrode, realize that assistant chemical titration etc. is a variety of advanced
Function.For the utility model, it can be achieved that diluting constant volume function simultaneously compared with previous various automatically or semi-automatically dilution instrument, dilution is smart
Degree and dilution efficiency higher；And compared with manual dilution, automatization level is high, dilution is simple and efficient, liquid it is totally-enclosed it is pollution-free,
The time for preparing solution, dilution precision and dilution efficiency higher can effectively be shortened, how many volume are actually needed in experiment
Solution to be measured just prepare how much, reduce the consumption of various reagents, can especially reduce various poisonous and harmful and volatile examination
The consumption of agent reduces damage of the experiment process to health, reduces experiment pollution on the environment.Again, from dilute
It releases and is seen in principle, the utility model controls double precision stepper motor by program and links, and realizes and determines in dilution breakthroughly
Hold function.In conclusion the utility model can well solve previous various automatically or semi-automatically dilution instrument and craft is dilute
Release existing various problems.
The utility model has the beneficial effects that：It is carried by totally-enclosed corrosion resistant flow pipe, two syringe double precisions
Take and distribute liquid, program controls the linkage of two stepper motors, this volum-controlled type automatic dilutor of the utility model can be with
Well solve previous various automatically or semi-automatically dilution instrument and by hand diluted shortcomings.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model.
In figure：1, micro syringe, 2, constant syringe, 3, seven port valves, 31, first passage, 32, second channel, 33,
Third channel, 34, fourth lane, 35, Five-channel, the 36, the 6th channel, the 37, the 7th channel, 4, triple valve, 41, channel one,
42, channel two, 43, channel three, 44, channel four, 5, quantitative loop, 6, line mixer.
Specific implementation mode
As shown in Figure 1, a kind of volum-controlled type automatic dilutor of the utility model includes a stepper motor driven micro note
The stepper motor driven constant syringe 2, one of emitter 1, one can seven port valve 3, one of free switching flow path can freely cut
4, quantitative loops 5 of four-way valve on change of current road and a line mixer 6 form.In the present embodiment, micro syringe uses
1000 μ L glass syringes.1000 μ L glass syringes are connected to 31 position of first passage of seven port valves.Constant syringe uses
25mL glass syringes.25mL glass syringes are connected to one 41 position of channel of four-way valve, between seven port valves 3 and four-way valve 4
It is together in series by the 6th channel 36 and channel 3 43, a bit of pipeline mixing is connected between the 6th channel 36 and channel 3 43
Device 6 becomes testing liquid after liquid and dilution to be diluted pass sequentially through seven port valves and four-way valve.
Access is explained in detail：First passage 31 connects the micro glass syringe of a 1000 μ L, and second channel 32 is to be diluted
Liquid extraction entrance, third channel 33 are that dilution extracts entrance, and fourth lane 34 is quantitative loop output channel, Five-channel 35
It is to match liquid passing away with four-way valve interface channel, the 7th channel to be micro for quantitative loop input channel, the 6th channel 36；5th
One section of quantitative loop 5 is connected between channel 35 and the 6th channel 36, Five-channel 35 is liquid inlet, and the 6th channel 36 is liquid
Outlet, the replaceable quantitative loop using not co-content（The generally 10 μ L ranges of μ L~1000）；6th channel 36 and channel 3 43
It is connected；It is connected with a bit of line mixer between 6th channel 36 and channel 3 43；One 25ml's of the connection of channel 1 is normal
Glass syringe is measured, channel 4 44 is that constant matches liquid passing away.
Performance：The minimum sample mass of micro syringe is 10 μ L, is up to 1000 μ L, and minimum interval is 1 μ L；Constant is injected
The minimum sample mass of device is 0.1mL, is up to 25mL, minimum interval 0.01mL.
The utility model has following items concrete function：
（1）It is micro to match liquid：Operation panel is set to be accumulated with liquid, at this point, second channel 32 is connected to first passage 31, step
Stepper motor driving micro syringe piston moves down a certain amount of liquid to be diluted of extraction, and then, second channel 32 is closed, and first
Channel 31 is connected to the 7th channel 37, and stepper motor driving micro syringe piston, which moves up, is discharged quantitative liquid（If carrying
Taking has bubble in pipeline, repeat this action for several times, extracted again after so that liquid to be diluted is full of pipeline）, the 7th channel 37 is row
Go out channel, second channel 32 and the 7th channel 37 can be connected to or close repeatedly with first passage 31, for cleaning, being full of repeatedly
Or empty syringe and pipeline.It is micro generally to prepare the 10 μ L testing liquids of μ L~1000 with liquid, can also micro syringe be made to live
Plug perseveration is multiple, matches liquid for 1000 μ L or more.
（2）Constant matches liquid：Operation panel is set to be accumulated with liquid, at this point, channel 2 42 is connected to channel 1, stepping electricity
Machine driving constant syringe piston moves down extraction certain quantity of fluid, and then, channel 2 42 is closed, channel 1 and channel four
44 connections, stepper motor driving constant syringe piston, which moves up, is discharged quantitative liquid（It, can if having bubble in extraction pipeline
It repeats this action for several times, liquid to be diluted is made then to be extracted again full of pipeline）.Channel 4 44 is passing away, 2 42 He of channel
Channel 4 44 can be connected to or close repeatedly with channel 1, for cleaning, being full of or emptying syringe and pipeline repeatedly.Constant
Can generally prepare 0.1mL~25mL testing liquids with liquid, can also make constant syringe piston perseveration multiple, for 25ml with
On match liquid.
（3）Microdilution（When the volume of required testing liquid is less）：The first step is to extract liquid to be diluted, first passage
32 are connected to first passage 31, and stepper motor driving micro syringe piston, which is moved down from second channel 32, extracts a certain amount of wait for
Diluent liquid, the then closing of second channel 32, stepper motor driving micro syringe piston is moved up to be pushed away from the 7th channel 37
Go out precisely quantitative liquid to be diluted to inject in a container（If having bubble in extraction pipeline, repeats this action for several times, make to wait for
Then diluent liquid is extracted again full of pipeline）；Second step is extraction dilution, at this point, third channel 33 connects with first passage 31
Logical, stepper motor driving micro syringe piston, which is moved down from third channel 33, extracts a certain amount of dilution, and then third is logical
Road 33 is closed, and stepper motor driving micro syringe piston, which is moved up, releases precisely quantitative dilution note from the 7th channel 37
Enter in the same container（If having bubble in extraction pipeline, this action is repeated for several times, dilution is made then to be carried again full of pipeline
It takes）.Finally, the liquid and dilution to be diluted in container are mixed, obtain testing liquid.Microdilution generally achievable 1~
100 times of arbitrary integer time dilution, also can make micro syringe piston reciprocating multiple, extract more dilutions, be used for 100 times
Above Microdilution, such as reciprocal 10 times, it can be achieved that 1000 times of Microdilutions.
（4）Constant dilutes（When the volume of required testing liquid is more）：The first step is to extract liquid to be diluted, channel 2 42
It is connected to channel 1, stepper motor driving constant syringe piston, which is moved down from channel 2 42, extracts a certain amount of liquid to be diluted
Body, then channel 2 42 close, channel 1 is connected to channel 4 44, and stepper motor drives constant syringe piston to move up
Precisely quantitative liquid to be diluted is discharged from channel 4 44 to inject in a container（If having bubble in extraction pipeline, this is repeated
Action for several times, makes liquid to be diluted then be extracted again full of pipeline）；Second step be extraction dilution, at this point, third channel 33 with
The connection of 6th channel 36, channel 3 43 are connected to channel 1, and stepper motor drives constant syringe piston to move down from the
Triple channel 33 extracts a certain amount of dilution, and then channel 3 43 is closed, and stepper motor drives constant syringe piston to moving up
It is dynamic to be discharged from channel 4 44 in the precisely quantitative same container of dilution injection（If having bubble in extraction pipeline, this is repeated
Action for several times, makes dilution then be extracted again full of pipeline）.Finally, the liquid to be diluted in container is mixed with dilution, is obtained
To testing liquid.The general arbitrary integer time dilution that can be achieved 1~250 times of constant dilution, also can make constant syringe piston past
It is multiple multiple, more dilutions are extracted, are diluted for 250 times or more of constants, such as reciprocal 10 times, it can be achieved that 2500 times of constants are dilute
It releases.
（5）High magnification dilutes：The first step is to extract liquid to be diluted, and second channel 32 is connected to first passage 31, stepping
Motor driving micro syringe piston, which is moved down from second channel 32, extracts a certain amount of liquid to be diluted, then second channel 32
It closes, stepper motor driving micro syringe piston is moved up, and precisely quantitative liquid to be diluted note is discharged from the 7th channel 37
Enter in a container（If having bubble in extraction pipeline, this action is repeated for several times, liquid to be diluted is made then to be carried again full of pipeline
It takes）；Second step is extraction dilution, and third channel 33 is connected to the 6th channel 36, channel 3 43 is connected to channel 1, stepping
Motor driving constant syringe piston, which is moved down from third channel 33, extracts a certain amount of dilution, and then channel 3 43 is closed
It closes, stepper motor driving constant syringe piston moves up that precisely quantitative dilution injection is same from the discharge of channel 4 44
In container（If having bubble in extraction pipeline, this action is repeated for several times, dilution is made then to be extracted again full of pipeline）.Finally,
Liquid and dilution to be diluted in container is mixed, testing liquid is obtained.Highly diluted multiplying power pattern generally can be achieved 1~2500
Times arbitrary integer time dilution, also can make constant syringe piston back and forth, extract more dilutions, for 2500 times with
On high magnification dilution, such as reciprocal 10 times, it can be achieved that 25000 times dilution.
（6）Quantitative loop dilutes：According to the needs of experiment, different appearances can be connected between fourth lane 34 and Five-channel 35
Long-pending quantitative loop, volume are generally differed from 10 μ L to 1000 μ L.When extracting liquid to be diluted, second channel 32 and first is logical first
Road 31 is connected to, and stepper motor driving micro syringe piston, which is moved down from second channel 32, extracts a certain amount of liquid to be diluted,
Then second channel 32 is closed, and first passage 31 is connected to Five-channel 35 simultaneously with fourth lane 34 and is connected to the 7th channel 37,
Stepper motor driving micro syringe piston, which moves up, is pushed into liquid to be diluted in quantitative loop, and extra liquid is led to by the 7th
Road 37 is discharged, and the liquid volume stored in quantitative loop at this time is exactly the volume of the quantitative loop, and then, first passage 31 is closed, the
Triple channel 33 is connected to fourth lane 34, Five-channel 35 is connected to the 6th channel 36, meanwhile, channel 3 43 connects with channel 1
Logical, 4 piston of stepper motor driving constant syringe moves down, meeting while extracting a certain amount of dilution from third channel 33
Liquid to be diluted in quantitative loop is brought into together in constant syringe 4, and constant volume is completed in constant syringe 4（It is to be diluted
Liquid and diluent stream by the road mixer 6 when obtained sufficient mixing）, finally, channel 3 43 is closed, channel 1 with it is logical
Road 4 44 is connected to, and stepper motor driving constant syringe piston moves up, and from the discharge dilution of channel 4 44, constant volume is good simultaneously
Testing liquid.
（7）Dilution while constant volume：It is mutually mixed suitable for two kinds of liquid of different nature, volume is accurate after constant volume, dilution
Precision is high, and dilution efficiency is high, can replace manually being operated with volumetric flask.Setting second channel 32 is connected to first passage 31, stepping
Motor driving micro syringe piston, which is moved down from second channel 32, extracts a certain amount of liquid to be diluted, then, second channel
32 close, third channel 33, the 6th channel 36,31 tripartite of first passage connection, while channel 3 43 is connected to channel 1, so
Afterwards, 3 piston of micro syringe moves up while 4 piston of constant syringe moves down, and dilution is extracted by third channel 33,
Liquid to be diluted is driven to enter in constant syringe 4 together,（Liquid to be diluted and diluent stream by the road mixer 6 when obtain
Sufficient mixing）, liquid to be diluted and dilution complete dilution constant volume simultaneously, then, channel 3 43 in constant syringe 4
It closes, channel 1 is connected to channel 4 44, and 4 piston of stepper motor driving constant syringe is moved up to be discharged from channel 4 44
Dilution while the good testing liquid of constant volume.1~2500 times of arbitrary integer time dilution generally can be achieved simultaneously in constant volume simultaneously for dilution
Constant volume also can make constant syringe piston back and forth, extract more dilutions, be used for 2500 times or more of dilution, such as past
Multiple 10 times, it can be achieved that 25000 times of dilutions.
（8）Automatic cleaning：It is logical second for cleaning cleaning micro syringe, constant syringe automatically and flowing through pipeline
Road 32, third channel 33 are connected to down simultaneously with first passage 31, micro syringe 3 from second channel 32, third channel 33 simultaneously
Cleaning solution is extracted, subsequent second channel 32, third channel 33 are turned off, and first passage 31 is connected to the 7th channel 37, from the 7th
Cleaning solution is discharged in channel 37, and, for cleaning micro syringe, the pipeline flowed through is cleaned together for this；Third channel 31 with
The connection of 6th channel 36, channel 3 43, channel 2 42 are connected to down with channel 1 simultaneously again, and constant syringe 4 is from third channel
33 and channel 2 42 extract cleaning solution simultaneously, subsequent channel 2 42, channel 3 43 are turned off, and channel 1 and channel 4 44 connect
It is logical, cleaning solution is discharged from channel 4 44, for cleaning constant syringe and line mixer 6, the pipeline flowed through obtains together for this
To cleaning, above-mentioned action can be repeated several times.
（9）Assistant chemical titrates：It needs to be used in combination with electrode appropriate, the tubes rows in the 7th channel 37 and channel 4 44
Exit needs installation liquid only to return head, then controls two stepper motors, first prerinse micro syringe 3 or constant syringe
4, make to be full of titrating solution in micro syringe 3 or constant syringe 4 and in discharge line, then by the 7th channel 37 or channel
4 44 pipeline outlet immerses in sample solution to be titrated, controls respective stepper motor driving 3 piston of micro syringe
Or 4 piston of constant syringe carries out accurate movement so that the titrating solution of accurate volume is discharged, and judges chemistry according to the reading of electrode
The terminal of titration calculates the result of titration.
Claims (6)
1. a kind of volum-controlled type automatic dilutor, it is characterised in that：Including a stepper motor driven micro syringe, a step
Stepper motor driving constant syringe, one can free switching flow path seven port valves and one can free switching flow path four-way
Valve；Seven port valve include for connect the first passage of micro syringe, for liquid to be diluted extract entrance second channel,
The third channel of entrance is extracted for dilution, is led to for the fourth lane of quantitative loop output, for the 5th of quantitative loop input
Road, the 6th channel for being connect with four-way valve and the 7th channel for micro distribution discharge；The four-way valve includes being used for
It connects the channel one of constant syringe, extract channel two, the channel three for being connect with seven port valves of entrance for liquid to be diluted
And match the channel four of liquid discharge for constant；The micro syringe is connected on the first passage, the constant injection
Device is connected on the channel one；It is connected with a certain amount of ring between the fourth lane and Five-channel；6th channel
It is connect by a line mixer with channel three-phase.
2. volum-controlled type automatic dilutor according to claim 1, it is characterised in that：Seven port valve and four-way valve have：
For micro the first functional status with liquid：First passage be connected with the second channel it is lower be used for extracting liq, subsequent the
Two channels are closed, and accurately micro liquid is discharged in the case where first passage is connected to the 7th channel；
Match the second functional status of liquid for constant：Extracting liq, subsequent channel two are used in the case where channel two is connected to channel one
It closes, accurately constant liquid is discharged in the case where channel one is connected to channel four；
Third functional status for Microdilution：It is connected under second channel and first passage for extracting liquid to be diluted,
Subsequent second channel is closed, and a certain amount of liquid to be diluted is discharged in the case where first passage is connected to the 7th channel；In third channel
Extraction dilution is connected to down with first passage, then third channel is closed, and one is discharged in the case where first passage is connected to the 7th channel
Quantitative dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 4th functional status of constant：Liquid to be diluted, subsequent channel are extracted in the case where channel two is connected to channel one
Two close, and a certain amount of liquid to be diluted is discharged in the case where channel one is connected to channel four；Be connected to the 6th channel in third channel,
Channel three is connected to down extraction dilution with channel one, and subsequent channel three is closed, and is discharged in the case where channel one is connected to channel four certain
The dilution of amount；Finally, by liquid to be diluted and dilution mixing；
For the diluted five-function state of high magnification：In the case where second channel is connected to first passage for extracting liquid to be diluted
Body, subsequent second channel are closed, and accurately micro liquid to be diluted is discharged in the case where first passage is connected to the 7th channel；In third
Channel is connected to the 6th channel, channel three is connected to down extraction dilution with channel one, and subsequent channel three is closed, and in channel one and is led to
The connection of road four is lower to be discharged accurately dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 6th functional status of quantitative loop：One section of quantitative loop is connected between fourth lane and Five-channel, the
Two channels are connected to down from second channel with first passage and extract liquid to be diluted, and subsequent second channel is closed, first passage with
Fourth lane be connected to and meanwhile Five-channel be connected to down with the 7th channel, by liquid to be diluted push-in quantitative loop in, surplus liquid by
7th channel is discharged, and the volume of the liquid stored in quantitative loop at this time is exactly the nominal volume of the quantitative loop, then, first passage
Be turned off with the 7th channel, third channel is connected to fourth lane, while Five-channel is connected to the 6th channel, and channel three with
Channel one is connected to, and extracts dilution from third channel, the liquid to be diluted in quantitative loop can go forward side by side with the extraction one of dilution
Enter constant syringe, liquid made to be completed at the same time dilution and constant volume in constant syringe, subsequent channel three is closed, channel one with it is logical
Road four is connected to, and the testing liquid diluted is discharged from channel four；
The 7th functional status for diluting constant volume simultaneously：In the case where second channel is connected to first passage, micro syringe is from
Two channel precisions extract liquid to be diluted, and fluid storage to be diluted is in micro syringe, and subsequent second channel is closed, in third
Channel, the 6th channel, first passage is connected to simultaneously, the 6th channel is connected to down with channel tee joint, channel three and channel one, constant
From third channel extraction dilution, liquid to be diluted is also pushed into connecting line syringe by micro syringe simultaneously, liquid to be diluted
Body is diluted liquid band and enters in pipeline, flows through line mixer and is mixed well, and constant volume is then completed in constant syringe, most
Afterwards, channel three is closed, and channel one is connected to channel four, from the discharge dilution of channel four while the good testing liquid of constant volume.
3. volum-controlled type automatic dilutor according to claim 2, it is characterised in that：Seven port valve and four-way valve also have
Have：
The 8th functional status for cleaning automatically：In the case where second channel, third channel are connected to simultaneously with first passage, micro note
Emitter from second channel, third channel simultaneously extract cleaning solution, subsequent second channel, third channel are turned off, first passage with
7th channel is connected to, and cleaning solution is discharged from the 7th channel, and, for cleaning micro syringe, the pipeline flowed through obtains clearly together for this
It washes；Be connected to the 6th channel in third channel, the 6th channel and channel tee joint, channel three and channel two again with channel one simultaneously
Under connection, constant syringe extracts cleaning solution simultaneously from third channel and channel two, and subsequent channel two, channel three are turned off, and leads to
Road one is connected to channel four, cleaning solution is discharged from channel four, this is flowed through for cleaning constant syringe and line mixer
Pipeline is cleaned together；
The 9th functional status for assistant chemical titration：It needs to be used in combination with electrode appropriate, the 7th channel and channel four
Tubes rows exit need install liquid only return head, then control two stepper motors, first prerinse micro syringe or often
Measure syringe, make to be full of titrating solution in micro syringe or constant syringe and in discharge line, then by the 7th channel or
The pipeline outlet in channel four immerses in sample solution to be titrated, controls respective stepper motor driving micro syringe piston
Or constant syringe piston carries out accurate movement so that the titrating solution of accurate volume is discharged, and judges chemistry drop according to the reading of electrode
Fixed terminal calculates the result of titration.
4. volum-controlled type automatic dilutor according to claim 3, it is characterised in that：The micro syringe and constant injection
Device is transparent glass syringe.
5. volum-controlled type automatic dilutor according to claim 4, it is characterised in that：The micro syringe is 1000 μ L.
6. volum-controlled type automatic dilutor according to claim 5, it is characterised in that：The constant syringe is 25mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820292516.1U CN208026537U (en) | 2018-03-02 | 2018-03-02 | A kind of volum-controlled type automatic dilutor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820292516.1U CN208026537U (en) | 2018-03-02 | 2018-03-02 | A kind of volum-controlled type automatic dilutor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208026537U true CN208026537U (en) | 2018-10-30 |
Family
ID=63905802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820292516.1U Withdrawn - After Issue CN208026537U (en) | 2018-03-02 | 2018-03-02 | A kind of volum-controlled type automatic dilutor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208026537U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108072557A (en) * | 2018-03-02 | 2018-05-25 | 南京科创检测技术有限公司 | A kind of volum-controlled type automatic dilutor |
| CN111381068A (en) * | 2018-12-27 | 2020-07-07 | 深圳市帝迈生物技术有限公司 | Liquid adding device, analyzer and liquid adding method |
| CN111521478A (en) * | 2020-05-20 | 2020-08-11 | 中国科学院深海科学与工程研究所 | Deep sea normal position gradient circulation diluting device |
-
2018
- 2018-03-02 CN CN201820292516.1U patent/CN208026537U/en not_active Withdrawn - After Issue
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108072557A (en) * | 2018-03-02 | 2018-05-25 | 南京科创检测技术有限公司 | A kind of volum-controlled type automatic dilutor |
| CN108072557B (en) * | 2018-03-02 | 2024-06-11 | 南京科创检测技术有限公司 | Constant volume type automatic dilution instrument |
| CN111381068A (en) * | 2018-12-27 | 2020-07-07 | 深圳市帝迈生物技术有限公司 | Liquid adding device, analyzer and liquid adding method |
| CN111521478A (en) * | 2020-05-20 | 2020-08-11 | 中国科学院深海科学与工程研究所 | Deep sea normal position gradient circulation diluting device |
| CN111521478B (en) * | 2020-05-20 | 2023-04-18 | 中国科学院深海科学与工程研究所 | Deep sea normal position gradient circulation diluting device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208026537U (en) | A kind of volum-controlled type automatic dilutor | |
| CN108072557A (en) | A kind of volum-controlled type automatic dilutor | |
| CN209296733U (en) | Sample analysis liquid channel system, sample analyser | |
| CN104062170A (en) | Automatic online micro-scale sample dilution and mixing system and mixing method | |
| CN215711746U (en) | Reagent filling device | |
| CN208902751U (en) | Quantitative sample injection and cleaning system | |
| CN221062327U (en) | Diluting cabinet for high-concentration liquid detection | |
| CN207457252U (en) | A kind of hemoglobin reaction unit and sample analyser | |
| CN212008633U (en) | Automatic sample feeding and preparing device for algae photosynthesis inhibition method water quality toxicity monitor | |
| CN211424010U (en) | Multi-range water quality analyzer flow path system | |
| CN207786607U (en) | A kind of evenly mixing device for test sample in analyzer reaction tank | |
| CN209979662U (en) | Flow injection reaction tank device and reversing fluid unit for same | |
| CN209166973U (en) | Liquid channel system and device for analyzing samples | |
| CN208297540U (en) | A kind of Flow Injection Analysis device for saving reagent solution | |
| CN109939636B (en) | Mixing device and method for test sample in analyzer reaction tank | |
| CN217652869U (en) | Quantitative assembly, sample processing device and reagent preparation device | |
| CN115616233A (en) | Online dilution device and method for flow injection analyzer | |
| CN202562846U (en) | Pneumatic flow sampling device for atomic fluorescence spectrometer | |
| CN104062169B (en) | A kind of automatic on-line micro-example dilution mixing device and mixed method | |
| CN108854837A (en) | On-line dilution device | |
| CN215877791U (en) | Liquid phase device capable of automatically proportioning and mixing | |
| CN208679061U (en) | On-line dilution device | |
| CN104062167A (en) | Automatic online sample dilution and mixing device and mixing method | |
| CN208800478U (en) | A kind of full automatic biochemical apparatus gush-type cleaning device | |
| CN203908852U (en) | Automatic online micro sample diluting and mixing system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20181030 Effective date of abandoning: 20240611 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20181030 Effective date of abandoning: 20240611 |