CN108072557A - A kind of volum-controlled type automatic dilutor - Google Patents
A kind of volum-controlled type automatic dilutor Download PDFInfo
- Publication number
- CN108072557A CN108072557A CN201810173889.1A CN201810173889A CN108072557A CN 108072557 A CN108072557 A CN 108072557A CN 201810173889 A CN201810173889 A CN 201810173889A CN 108072557 A CN108072557 A CN 108072557A
- Authority
- CN
- China
- Prior art keywords
- passage
- liquid
- channel
- diluted
- syringe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 claims abstract description 141
- 239000012895 dilution Substances 0.000 claims abstract description 101
- 238000010790 dilution Methods 0.000 claims abstract description 101
- 238000000605 extraction Methods 0.000 claims abstract description 35
- 238000009826 distribution Methods 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims description 25
- 239000000284 extract Substances 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 22
- 238000012360 testing method Methods 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 13
- 239000011521 glass Substances 0.000 claims description 11
- 238000004448 titration Methods 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000007865 diluting Methods 0.000 claims description 3
- 239000012488 sample solution Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 2
- 238000000520 microinjection Methods 0.000 abstract 1
- 230000009471 action Effects 0.000 description 10
- 239000000463 material Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000007797 corrosion Effects 0.000 description 5
- 238000005260 corrosion Methods 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 238000005213 imbibition Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 230000007096 poisonous effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 239000003049 inorganic solvent Substances 0.000 description 2
- 229910001867 inorganic solvent Inorganic materials 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229920000306 polymethylpentene Polymers 0.000 description 2
- 239000011116 polymethylpentene Substances 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 206010034703 Perseveration Diseases 0.000 description 1
- QYKIQEUNHZKYBP-UHFFFAOYSA-N Vinyl ether Chemical compound C=COC=C QYKIQEUNHZKYBP-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 125000005816 fluoropropyl group Chemical group [H]C([H])(F)C([H])([H])C([H])([H])* 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 125000005010 perfluoroalkyl group Chemical group 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of volum-controlled type automatic dilutor, including a stepper motor driven micro syringe, a stepper motor driven constant syringe, one can free switching flow path seven port valves and one can free switching flow path four-way valve；Seven port valves include connecting the first passage of micro-injection, second channel, the third channel for dilution extraction entrance, the fourth lane for rationed output for liquid to be diluted extraction entrance, the Five-channel for rationed input, the 6th passage for being connected with triple valve and the 7th passage for micro distribution discharge；Four-way valve includes connecting the passage one of constant syringe, the passage two of entrance is extracted for liquid to be diluted, for the passage three being connected with seven port valves, the passage four discharged for constant with liquid；Micro syringe connects on the first pass, and constant syringe is connected on passage one, the 6th passage and passage three is connected, centre is connected with a bit of line mixer.The present invention well solves previous various dilution instrument and manual diluted shortcomings by the linkage of double precision syringe, two stepper motors.
Description
Technical field
The present invention relates to a kind of volum-controlled type automatic dilutors, are widely used in all analysis test industries, it can be achieved that a variety of liquid
Body sample carries out that High Precision Automatic constant volume, automatic cleaning and assistant chemical titration etc. are many simultaneously with liquid, automatic dilution, dilution
Advanced function.
Background technology
At present, existing liquid automatic dilutor brand is numerous both at home and abroad, and the LH-025 types of such as domestic labhands brands are automatic
Dilute instrument, the OREM-2000 type automatic dilutors of labhands brands, German He Shiman（Hirschmann）The electronics bottle of brand
Mouth dispenser, German Brand brands electronics bottleneck dispenser, domestic big dragon electronics bottleneck dispenser, in addition, also domestic Beijing
600 series of MicroLab of the clear 6D four-in-one automated fluid sample adding systems for grinding think of wound and U.S.'s Hamilton brands is automatic
Dilution instrument dilution instrument etc..These automatic dilutors are only applicable to inorganic solvent or are only applicable to organic solvent, once only
A kind of liquid can be drawn, accurate simultaneously can not draw organic solvent and inorganic solvent；And the sample volume of electronics bottleneck dispenser
The factors such as environment temperature, liquid viscosity, liquid property, surface tension are additionally depended on, it is not also very high, and general to cause sampling precision
Logical bottleneck dispenser is only used for quantitative liquid separating, without dilution function；In terms of dilution step process, these automatic dilutors
Principle be all the then liquid relief first with negative pressure precision imbibition, dilution efficiency is low, and dilution and constant volume cannot be carried out at the same time, and profit
It is easy to generate bubble with negative-pressure liquid suction, since the elastic module of air is big, extra air is also easy to cause suction in imbibition chamber
The inaccuracy of liquid product.In addition, from dilution principle, the automatic dilutor of previous various brands can accomplish automatic precision
Imbibition and liquid relief can not but accomplish accurate constant volume, and reason is after two different kinds of liquid mixing, due to intermolecular distance
It changes, more or less variation can inevitably occur for total volume, especially when a kind of inorganic liquid and one kind are organic
After liquid mixing, simple addition that total volume would generally reduce without being two kinds of liquid volumes, for example 100ml pure water with
Volume is significantly less than 200ml to 100ml ethyl alcohol after mixing（About 194ml）.Again, previous automatic dilutor usually only has
There is single syringe to be used for extracting liq, extension rate scope is small, single precision, and practical effect is bad, frequency of use is not high,
And have simultaneously there are two syringe, the double precision automatic dilutor of the liquid of accurate extraction different volumes can be distinguished, it is for example beautiful
The serial automatic dilutors of MicroLab 600 of state's Hamilton brands, then it is expensive, it can not largely popularize.In conclusion
Existing various automatically or semi-automatically dilution instrument can not achieve the accurate function of diluting constant volume simultaneously both at home and abroad at present, can not substitute
The work manually completed using volumetric flask during specification is examined, thus can not largely use, and dilute that there are testing crews by hand
Maloperation, dilution efficiency is low, dilutes low precision, dilutes poisonous and harmful and effumability liquid hazard test personnel health, matches somebody with somebody
Extra reagent or solution cause to waste, pollute the shortcomings such as environment during system.
The content of the invention
For at present both at home and abroad it is existing it is various automatically or semi-automatically dilute instrument generally existings can only accurate imbibition and liquid relief, no
Can accurate dilution constant volume, the liquid volume of extraction are not enough precisely diluted by hand there are testing crew maloperation, diluted simultaneously
Efficiency is low, poisonous and harmful and effumability liquid hazard test personnel health, process for preparation in dilution low precision, dilution
In extra reagent or solution cause to waste, pollute the shortcomings such as environment, a kind of volum-controlled type automatic dilution of the present invention
Instrument can well solve the above problem.
Technical solution is used by the present invention solves above-mentioned technical problem：
A kind of volum-controlled type automatic dilutor, it is characterised in that：Including a stepper motor driven micro syringe, a stepping
Motor-driven constant syringe, one can free switching flow path seven port valves and one can free switching flow path four-way valve；
Seven port valve include for connect micro syringe first passage, for liquid extraction entrance to be diluted second channel,
The third channel of entrance is extracted for dilution, is led to for the fourth lane of quantitative loop output, for the 5th of quantitative loop input
Road, the 6th passage for being connected with four-way valve and the 7th passage for micro distribution discharge；The four-way valve includes using
In connection constant syringe passage one, for dilution extraction entrance passage two, for be connected with seven port valves passage three,
For the passage four of constant distribution discharge；The micro syringe is connected on the first passage, and the constant syringe connects
It is connected on the passage one；A certain amount of ring is connected between the fourth lane and Five-channel；6th passage and logical
Road three is connected, centre is connected with a bit of line mixer.
Seven port valve and four-way valve have：
For micro the first functional status with liquid：In the case where first passage is connected with second channel for extracting liq, subsequent the
Two passages are closed, and accurately micro liquid is discharged in the case where first passage is connected with the 7th passage；
Match somebody with somebody the second functional status of liquid for constant：In the case where passage two is connected with passage one for extracting liq, subsequent passage two
It closes, accurately constant liquid is discharged in the case where passage one is connected with passage four；
For the 3rd functional status of Microdilution：In second channel with being connected under first passage for extracting liquid to be diluted,
Subsequent second channel is closed, and a certain amount of liquid to be diluted is discharged in the case where first passage is connected with the 7th passage；In third channel
With connecting to extract dilution under first passage, subsequent third channel is closed, arranged in the case where first passage is connected with the 7th passage
Go out a certain amount of dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 4th functional status of constant：Liquid to be diluted, subsequent passage are extracted in the case where passage two is connected with passage one
Two close, and passage one connects a certain amount of liquid to be diluted of discharge with passage four；It is connected with the 6th passage in third channel, passage
Three connect down extraction dilution with passage one, and then passage three is closed, and is discharged in the case where passage one is connected with passage four a certain amount of
Dilution；Finally, by liquid to be diluted and dilution mixing；
For the diluted five-function state of high magnification：It is used to extract liquid to be diluted in the case where second channel is connected with first passage
Body, subsequent second channel are closed, and accurately micro liquid to be diluted is discharged in the case where first passage is connected with the 7th passage；The 3rd
Passage is connected with the 6th passage, passage three connects down extraction dilution with passage one, and subsequent passage three is closed, in passage one with leading to
The connection of road four is lower to discharge accurately dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 6th functional status of quantitative loop：One section of quantitative loop is connected between fourth lane and Five-channel, the
Two passages connect down from second channel with first passage and extract liquid to be diluted, and subsequent second channel is closed, first passage with
Fourth lane connect and meanwhile Five-channel connected down with the 7th passage, by liquid to be diluted push-in quantitative loop in, surplus liquid by
7th passage is discharged, and the volume of the liquid stored at this time in quantitative loop is exactly the nominal volume of the quantitative loop, then, first passage
Be turned off with the 7th passage, third channel is connected with fourth lane, while Five-channel is connected with the 6th passage, and passage three with
Passage one connects, and from third channel extraction dilution, the liquid to be diluted in quantitative loop can go forward side by side with the extraction one of dilution
Enter constant syringe, make liquid in constant syringe while complete dilution and constant volume, subsequent passage three are closed, passage one with it is logical
Road four connects, and the testing liquid diluted is discharged from passage four；
For diluting the 7th functional status of constant volume simultaneously：In the case where second channel is connected with first passage, micro syringe is from
Two passages are accurate to extract liquid to be diluted, and fluid storage to be diluted is in micro syringe, and subsequent second channel is closed, the 3rd
Passage, the 6th passage, first passage connect simultaneously, the 6th passage connects down with passage tee joint, passage three and passage one, constant
From third channel extraction dilution, liquid to be diluted is also pushed into connecting line syringe by micro syringe simultaneously, liquid to be diluted
Body is diluted liquid band and enters in pipeline, flows through line mixer and obtains abundant mixing, constant volume is then completed in constant syringe, most
Afterwards, passage three is closed, and passage one is connected with passage four, from the discharge of passage four dilution while the good testing liquid of constant volume.
Seven port valve and four-way valve also have：
For the 8th functional status cleaned automatically：In the case where second channel, third channel connect simultaneously with first passage, micro note
Emitter extracts cleaning solution simultaneously from second channel, third channel, and subsequent second channel, third channel are turned off, first passage with
7th passage connects, and cleaning solution is discharged from the 7th passage, and, for cleaning micro syringe, the pipeline flowed through obtains clearly together for this
It washes；Connected with the 6th passage in third channel, the 6th passage and passage tee joint, passage three and passage two again with passage one simultaneously
Under connection, constant syringe extracts cleaning solution simultaneously from third channel and passage two, and subsequent passage two, passage three are turned off, and leads to
Road one is connected with passage four, discharges cleaning solution from passage four, this is flowed through for cleaning constant syringe and line mixer
Pipeline is cleaned together.
For the 9th functional status of assistant chemical titration：It needs to be used in combination with appropriate electrode, the 7th passage and logical
The tubes rows exit in road four needs installation liquid only to return head, then controls two stepper motors, first prerinse micro syringe
Or constant syringe, make to be full of titrating solution in micro syringe or constant syringe and in discharge line, then lead to the 7th
The pipeline outlet of road or passage four is immersed in sample solution to be titrated, controls respective driving stepper motor micro syringe
Piston or constant syringe piston carry out accurate movement to discharge the titrating solution of accurate volume, according to the reading judgementization of electrode
The terminal of titration is learned, calculates the result of titration.
Volum-controlled type automatic dilutor of the present invention mainly by the micro glass syringe of precision stepper motor driving, one
Precision stepper motor driving constant glass syringe, one can free switching flow path seven port valves, one can free switching stream
The four-way valve on road, a line mixer are formed.Can realize double precision with liquid, double precision dilution, dilution simultaneously constant volume, with fixed
A variety of advanced functions such as amount ring is diluted, cleans automatically, assistant chemical titration.
The syringe piston of the present invention and the nipple of each flow pipe are made of resistant material.Flow pipe
It need to use corrosion-resistant and be made with certain permeability, flexible material, can both be resistant to a variety of organic reagents and inorganic
Reagent can easily observe the mobility status of liquid again, have a situations such as bubble-free, the alternative costs of flow pipe need it is relatively low and
Facilitate replacement.Polypropylene can be used in flow pipe（PP）Material can also use polymethylpentene as needed（PMP）Material is complete
The copolymer of fluoropropyl perfluoroalkyl vinyl ether and polytetrafluoroethylene (PTFE)（PFA）Material, equally with transparent characteristic, and it is corrosion-resistant
Property, heat resistance are stronger, but cost is higher.Sealing ring is using elastic resistant material.Injector wall need to be used corrosion-resistant and had
There is the transparent glass material of super-low expansion coefficient, to ensure that the liquid volume of extraction is accurate, facilitate observation.
The applicable field of the present invention is wide, frequency of use is high, dilution efficiency is high, extension rate scope is wide, corrosion-resistant, anti-pollution
It contaminates, is not easy to remain, can clean automatically, moreover it is possible to be used in combination with suitable electrode, realize a variety of advanced work(such as assistant chemical titration
Energy.It is of the invention to be compared with the previous various instrument that automatically or semi-automatically dilute, it can be achieved that dilution constant volume function simultaneously, dilution precision and dilute
It releases more efficient；And compared with manual dilution, automatization level is high, dilutes simple and efficient, the totally-enclosed pollution-free, Ke Yiyou of liquid
Effect ground shortens the time for preparing solution, dilution precision and dilution efficiency higher, and the to be measured molten of how many volume is actually needed in experiment
Liquid just prepare how much, reduce the consumption of various reagents, can especially reduce disappearing for various poisonous and harmful and volatile reagent
Consumption, reduces damage of the experiment process to health, reduces experiment pollution on the environment.Again, from dilution principle
On see, the present invention by programme-control double precision stepper motor link, realize breakthroughly while dilution while constant volume function.To sum up
Described, the present invention can well solve various existing for previous various automatically or semi-automatically dilution instrument and manual dilution ask
Topic.
The beneficial effects of the invention are as follows：By totally-enclosed corrosion resistant flow pipe, the extraction of two syringe double precisions and
The linkage of two liquid, programme-control stepper motors is distributed, this volum-controlled type automatic dilutor of the invention can solve well
Certainly previous various automatically or semi-automatically dilution instrument and by hand diluted shortcomings.
Description of the drawings
Fig. 1 is the structure diagram of the present invention.
In figure：1st, micro syringe, 2, constant syringe, 3, seven port valves, 31, first passage, 32, second channel, 33,
Third channel, 34, fourth lane, 35, Five-channel, the 36, the 6th passage, the 37, the 7th passage, 4, triple valve, 41, passage one,
42nd, passage two, 43, passage three, 44, passage four, 5, quantitative loop, 6, line mixer.
Specific embodiment
As shown in Figure 1, a kind of volum-controlled type automatic dilutor of the present invention includes a stepper motor driven micro syringe
1st, stepper motor driven constant syringe 2, one can seven port valve 3, one of free switching flow path can free switching stream
4, quantitative loops 5 of four-way valve on road and a line mixer 6 form.In the present embodiment, micro syringe uses 1000 μ
L glass syringes.1000 μ L glass syringes are connected to 31 position of first passage of seven port valves.Constant syringe uses 25mL glass
Glass syringe.25mL glass syringes are connected to one 41 position of passage of four-way valve, pass through between seven port valves 3 and four-way valve 4
Six passages 36 and passage 3 43 are together in series, and a bit of line mixer 6 is connected between the 6th passage 36 and passage 3 43, is treated
After diluent liquid and dilution pass sequentially through seven port valves and four-way valve, become testing liquid.
Access is explained in detail：First passage 31 connects the micro glass syringe of a 1000 μ L, and second channel 32 is to be diluted
Liquid extraction entrance, third channel 33 extract entrance for dilution, and fourth lane 34 is quantitative loop output channel, Five-channel 35
It is to match somebody with somebody liquid passing away with four-way valve interface channel, the 7th passage to be micro for quantitative loop input channel, the 6th passage 36；5th
One section of quantitative loop 5 is connected between 35 and the 6th passage 36 of passage, Five-channel 35 is liquid inlet, and the 6th passage 36 is liquid
Outlet, the replaceable quantitative loop using not co-content（The μ L scopes of generally 10 μ L~1000）；6th passage 36 and passage 3 43
It is connected；A bit of line mixer is connected between 6th passage 36 and passage 3 43；One 25ml's of the connection of passage 1 is normal
Glass syringe is measured, passage 4 44 matches somebody with somebody liquid passing away for constant.
Performance：The minimum sample mass of micro syringe is 10 μ L, is up to 1000 μ L, and minimum interval is 1 μ L；Constant is injected
The minimum sample mass of device is 0.1mL, is up to 25mL, minimum interval 0.01mL.
The present invention has following items concrete function：
（1）It is micro to match somebody with somebody liquid：Operation panel is set to be accumulated with liquid, at this point, second channel 32 is connected with first passage 31, stepping electricity
Machine driving micro syringe piston moves down a certain amount of liquid to be diluted of extraction, and then, second channel 32 is closed, first passage
31 connect with the 7th passage 37, and driving stepper motor micro syringe piston, which moves up, discharges quantitative liquid（If extraction tube
There is bubble in road, repeat this action for several times, liquid to be diluted is made to be extracted again after being full of pipeline）, the 7th passage 37 is that discharge is logical
Road, 32 and the 7th passage 37 of second channel can connect or close repeatedly with first passage 31, for cleaning, being full of repeatedly or arrange
Empty syringe and pipeline.It is micro generally to prepare the μ L testing liquids of 10 μ L~1000 with liquid, it can also make micro syringe piston anti-
Double action is made repeatedly, matches somebody with somebody liquid for more than 1000 μ L.
（2）Constant matches somebody with somebody liquid：Operation panel is set to be accumulated with liquid, at this point, passage 2 42 is connected with passage 1, stepping electricity
Machine driving constant syringe piston moves down extraction certain quantity of fluid, and then, passage 2 42 is closed, passage 1 and passage four
44 connections, driving stepper motor constant syringe piston, which moves up, discharges quantitative liquid（It, can if having bubble in extraction pipeline
It repeats this action for several times, makes liquid to be diluted full of pipeline and then extract again）.Passage 4 44 be passing away, 2 42 He of passage
Passage 4 44 can connect or close repeatedly with passage 1, for cleaning, being full of repeatedly or empty syringe and pipeline.Constant
Can generally prepare 0.1mL~25mL testing liquids with liquid, can also make constant syringe piston perseveration multiple, for 25ml with
On match somebody with somebody liquid.
（3）Microdilution（When the volume of required testing liquid is less）：The first step is to extract liquid to be diluted, first passage
32 connect with first passage 31, and driving stepper motor micro syringe piston, which is moved down from second channel 32, extracts a certain amount of treat
Diluent liquid, then second channel 32 close, driving stepper motor micro syringe piston is moved up to be pushed away from the 7th passage 37
Go out precisely quantitative liquid to be diluted to inject in a container（If having bubble in extraction pipeline, repeat this action for several times, make to treat
Diluent liquid is full of pipeline and then extracts again）；Second step is extraction dilution, at this point, third channel 33 connects with first passage 31
Logical, driving stepper motor micro syringe piston, which is moved down from third channel 33, extracts a certain amount of dilution, then threeway
Road 33 is closed, and driving stepper motor micro syringe piston, which is moved up, releases precisely quantitative dilution note from the 7th passage 37
Enter in same container（If having bubble in extraction pipeline, repeat this action for several times, make dilution full of pipeline and then carry again
It takes）.Finally, the liquid and dilution to be diluted in container are mixed, obtain testing liquid.Microdilution generally achievable 1~
100 times of arbitrary integer time dilution, can also make micro syringe piston reciprocating multiple, more dilutions be extracted, for 100 times
Above Microdilution, such as reciprocal 10 times, it can be achieved that 1000 times of Microdilutions.
（4）Constant dilutes（When the volume of required testing liquid is more）：The first step is to extract liquid to be diluted, passage 2 42
It is connected with passage 1, driving stepper motor constant syringe piston moves down from passage 2 42 and extracts a certain amount of liquid to be diluted
Body, then passage 2 42 close, passage 1 connects with passage 4 44, and driving stepper motor constant syringe piston moves up
Precisely quantitative liquid to be diluted is discharged from passage 4 44 to inject in a container（If having bubble in extraction pipeline, this is repeated
Action for several times, makes liquid to be diluted full of pipeline and then extracts again）；Second step for extraction dilution, at this point, third channel 33 with
6th passage 36 connects, passage 3 43 is connected with passage 1, and driving stepper motor constant syringe piston is moved down from
Triple channel 33 extracts a certain amount of dilution, and then passage 3 43 is closed, and driving stepper motor constant syringe piston is to moving up
It is dynamic to be discharged from passage 4 44 in the precisely quantitative same container of dilution injection（If having bubble in extraction pipeline, this is repeated
Action for several times, makes dilution full of pipeline and then extracts again）.Finally, the liquid to be diluted in container with dilution is mixed, obtained
To testing liquid.The general arbitrary integer time dilution that can be achieved 1~250 times of constant dilution, can also make constant syringe piston past
It is multiple multiple, more dilutions are extracted, are diluted for 250 times or more of constants, such as reciprocal 10 times, it can be achieved that 2500 times of constants are dilute
It releases.
（5）High magnification dilutes：The first step is extracts liquid to be diluted, and second channel 32 is connected with first passage 31, stepping
Motor driving micro syringe piston, which is moved down from second channel 32, extracts a certain amount of liquid to be diluted, then second channel 32
It closes, driving stepper motor micro syringe piston, which is moved up, discharges precisely quantitative liquid to be diluted note from the 7th passage 37
Enter in a container（If having bubble in extraction pipeline, repeat this action for several times, make liquid to be diluted full of pipeline and then carry again
It takes）；Second step is extraction dilution, and third channel 33 is connected with the 6th passage 36, passage 3 43 is connected with passage 1, stepping
Motor driving constant syringe piston, which is moved down from third channel 33, extracts a certain amount of dilution, and then passage 3 43 closes
It closes, driving stepper motor constant syringe piston moves up that precisely quantitative dilution injection is same from the discharge of passage 4 44
In container（If having bubble in extraction pipeline, repeat this action for several times, make dilution full of pipeline and then extract again）.Finally,
Liquid and dilution to be diluted in container is mixed, obtains testing liquid.Highly diluted multiplying power pattern generally can be achieved 1~2500
Times arbitrary integer time dilution, also can make constant syringe piston back and forth, extract more dilutions, for 2500 times with
On high magnification dilution, such as reciprocal 10 times, it can be achieved that 25000 times dilution.
（6）Quantitative loop dilutes：According to the needs of experiment, different appearances can be connected between fourth lane 34 and Five-channel 35
Long-pending quantitative loop, volume are generally differed from 10 μ L to 1000 μ L.When extracting liquid to be diluted, second channel 32 and first is logical first
Road 31 connects, and driving stepper motor micro syringe piston moves down from second channel 32 and extracts a certain amount of liquid to be diluted,
Then second channel 32 is closed, and first passage 31 connects Five-channel 35 simultaneously with fourth lane 34 and connected with the 7th passage 37,
Driving stepper motor micro syringe piston, which moves up, is pushed into liquid to be diluted in quantitative loop, and extra liquid is led to by the 7th
Road 37 is discharged, and the liquid volume stored at this time in quantitative loop is exactly the volume of the quantitative loop, and then, first passage 31 is closed, the
Triple channel 33 is connected with fourth lane 34, Five-channel 35 is connected with the 6th passage 36, meanwhile, passage 3 43 connects with passage 1
Logical, 4 piston of driving stepper motor constant syringe moves down, meeting while extracting a certain amount of dilution from third channel 33
Liquid to be diluted in quantitative loop is brought into together in constant syringe 4, and constant volume is completed in constant syringe 4（It is to be diluted
Liquid and diluent stream by the road mixer 6 when obtained sufficient mixing）, finally, passage 3 43 is closed, and passage 1 is with leading to
Road 4 44 connects, and driving stepper motor constant syringe piston moves up, and from the discharge of passage 4 44 dilution, constant volume is good simultaneously
Testing liquid.
（7）Dilution while constant volume：It is mutually mixed suitable for two kinds of liquid of different nature, volume is accurate after constant volume, dilution
Precision is high, and dilution efficiency is high, can replace manually being operated with volumetric flask.Second channel 32 is set to be connected with first passage 31, stepping
Motor driving micro syringe piston, which is moved down from second channel 32, extracts a certain amount of liquid to be diluted, then, second channel
32 close, third channel 33, the 6th passage 36,31 tripartite of first passage connection, while passage 3 43 is connected with passage 1, so
Afterwards, 3 piston of micro syringe moves up while 4 piston of constant syringe moves down, and dilution is extracted by third channel 33,
Liquid to be diluted is driven to enter together in constant syringe 4,（Liquid to be diluted and diluent stream by the road mixer 6 when obtain
Sufficient mixing）, liquid to be diluted and dilution complete dilution constant volume simultaneously, then, passage 3 43 in constant syringe 4
It closes, passage 1 is connected with passage 4 44, and 4 piston of driving stepper motor constant syringe is moved up discharges from passage 4 44
Dilution while the good testing liquid of constant volume.1~2500 times of arbitrary integer time dilution generally can be achieved simultaneously in constant volume simultaneously for dilution
Constant volume also can make constant syringe piston back and forth, extract more dilutions, such as past for 2500 times or more of dilution
Multiple 10 times, it can be achieved that 25000 times of dilutions.
（8）Automatic cleaning：It is logical second for cleaning cleaning micro syringe, constant syringe automatically and flowing through pipeline
Road 32, third channel 33 connect down simultaneously with first passage 31, micro syringe 3 from second channel 32, third channel 33 simultaneously
Cleaning solution is extracted, subsequent second channel 32, third channel 33 are turned off, and first passage 31 is connected with the 7th passage 37, from the 7th
Passage 37 discharges cleaning solution, and, for cleaning micro syringe, the pipeline flowed through is cleaned together for this；Third channel 31 with
6th passage 36 connects, passage 3 43, passage 2 42 connect down simultaneously with passage 1 again, and constant syringe 4 is from third channel
33 and passage 2 42 extract cleaning solution simultaneously, subsequent passage 2 42, passage 3 43 are turned off, and passage 1 and passage 4 44 connect
It is logical, cleaning solution is discharged from passage 4 44, for cleaning constant syringe and line mixer 6, the pipeline flowed through obtains together for this
To cleaning, above-mentioned action can be repeated several times.
（9）Assistant chemical titrates：It needs to be used in combination with appropriate electrode, the tubes rows of the 7th passage 37 and passage 4 44
Exit needs installation liquid only to return head, then controls two stepper motors, first prerinse micro syringe 3 or constant syringe
4, make to be full of titrating solution in micro syringe 3 or constant syringe 4 and in discharge line, then by the 7th passage 37 or passage
4 44 pipeline outlet is immersed in sample solution to be titrated, controls respective 3 piston of driving stepper motor micro syringe
Or 4 piston of constant syringe carries out accurate movement to discharge the titrating solution of accurate volume, judges chemistry according to the reading of electrode
The terminal of titration calculates the result of titration.
Claims (6)
1. a kind of volum-controlled type automatic dilutor, it is characterised in that：Including a stepper motor driven micro syringe, a step
Stepper motor driving constant syringe, one can free switching flow path seven port valves and one can free switching flow path four-way
Valve；Seven port valve include for connect micro syringe first passage, for liquid to be diluted extraction entrance second channel,
The third channel of entrance is extracted for dilution, is led to for the fourth lane of quantitative loop output, for the 5th of quantitative loop input
Road, the 6th passage for being connected with four-way valve and the 7th passage for micro distribution discharge；The four-way valve includes being used for
Passage one, passage two, the passage three for being connected with seven port valves for liquid to be diluted extraction entrance for connecting constant syringe
And match somebody with somebody the passage four of liquid discharge for constant；The micro syringe is connected on the first passage, the constant injection
Device is connected on the passage one；A certain amount of ring is connected between the fourth lane and Five-channel；6th passage
It is connected with passage three-phase by a line mixer.
2. volum-controlled type automatic dilutor according to claim 1, it is characterised in that：Seven port valve and four-way valve have：
For micro the first functional status with liquid：In the case where first passage is connected with second channel for extracting liq, subsequent the
Two passages are closed, and accurately micro liquid is discharged in the case where first passage is connected with the 7th passage；
Match somebody with somebody the second functional status of liquid for constant：In the case where passage two is connected with passage one for extracting liq, subsequent passage two
It closes, accurately constant liquid is discharged in the case where passage one is connected with passage four；
For the 3rd functional status of Microdilution：In second channel with being connected under first passage for extracting liquid to be diluted,
Subsequent second channel is closed, and a certain amount of liquid to be diluted is discharged in the case where first passage is connected with the 7th passage；In third channel
Extraction dilution is connected down with first passage, then third channel is closed, and one is discharged in the case where first passage is connected with the 7th passage
Quantitative dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 4th functional status of constant：Liquid to be diluted, subsequent passage are extracted in the case where passage two is connected with passage one
Two close, and a certain amount of liquid to be diluted is discharged in the case where passage one is connected with passage four；Connected with the 6th passage in third channel,
Passage three connects down extraction dilution with passage one, and subsequent passage three is closed, and is discharged in the case where passage one is connected with passage four certain
The dilution of amount；Finally, by liquid to be diluted and dilution mixing；
For the diluted five-function state of high magnification：It is used to extract liquid to be diluted in the case where second channel is connected with first passage
Body, subsequent second channel are closed, and accurately micro liquid to be diluted is discharged in the case where first passage is connected with the 7th passage；The 3rd
Passage is connected with the 6th passage, passage three connects down extraction dilution with passage one, and subsequent passage three is closed, in passage one with leading to
The connection of road four is lower to discharge accurately dilution；Finally, by liquid to be diluted and dilution mixing；
For diluted 6th functional status of quantitative loop：One section of quantitative loop is connected between fourth lane and Five-channel, the
Two passages connect down from second channel with first passage and extract liquid to be diluted, and subsequent second channel is closed, first passage with
Fourth lane connect and meanwhile Five-channel connected down with the 7th passage, by liquid to be diluted push-in quantitative loop in, surplus liquid by
7th passage is discharged, and the volume of the liquid stored at this time in quantitative loop is exactly the nominal volume of the quantitative loop, then, first passage
Be turned off with the 7th passage, third channel is connected with fourth lane, while Five-channel is connected with the 6th passage, and passage three with
Passage one connects, and from third channel extraction dilution, the liquid to be diluted in quantitative loop can go forward side by side with the extraction one of dilution
Enter constant syringe, make liquid in constant syringe while complete dilution and constant volume, subsequent passage three are closed, passage one with it is logical
Road four connects, and the testing liquid diluted is discharged from passage four；
For diluting the 7th functional status of constant volume simultaneously：In the case where second channel is connected with first passage, micro syringe is from
Two passages are accurate to extract liquid to be diluted, and fluid storage to be diluted is in micro syringe, and subsequent second channel is closed, the 3rd
Passage, the 6th passage, first passage connect simultaneously, the 6th passage connects down with passage tee joint, passage three and passage one, constant
From third channel extraction dilution, liquid to be diluted is also pushed into connecting line syringe by micro syringe simultaneously, liquid to be diluted
Body is diluted liquid band and enters in pipeline, flows through line mixer and obtains abundant mixing, constant volume is then completed in constant syringe, most
Afterwards, passage three is closed, and passage one is connected with passage four, from the discharge of passage four dilution while the good testing liquid of constant volume.
3. volum-controlled type automatic dilutor according to claim 2, it is characterised in that：Seven port valve and four-way valve also have
Have：
For the 8th functional status cleaned automatically：In the case where second channel, third channel connect simultaneously with first passage, micro note
Emitter extracts cleaning solution simultaneously from second channel, third channel, and subsequent second channel, third channel are turned off, first passage with
7th passage connects, and cleaning solution is discharged from the 7th passage, and, for cleaning micro syringe, the pipeline flowed through obtains clearly together for this
It washes；Connected with the 6th passage in third channel, the 6th passage and passage tee joint, passage three and passage two again with passage one simultaneously
Under connection, constant syringe extracts cleaning solution simultaneously from third channel and passage two, and subsequent passage two, passage three are turned off, and leads to
Road one is connected with passage four, discharges cleaning solution from passage four, this is flowed through for cleaning constant syringe and line mixer
Pipeline is cleaned together；
For the 9th functional status of assistant chemical titration：It needs to be used in combination with appropriate electrode, the 7th passage and passage four
Tubes rows exit need install liquid only return head, then control two stepper motors, first prerinse micro syringe or often
Measure syringe, make in micro syringe or constant syringe and in discharge line full of titrating solution, then by the 7th passage or
The pipeline outlet of passage four is immersed in sample solution to be titrated, controls respective driving stepper motor micro syringe piston
Or constant syringe piston carries out accurate movement to discharge the titrating solution of accurate volume, judges chemistry drop according to the reading of electrode
Fixed terminal calculates the result of titration.
4. volum-controlled type automatic dilutor according to claim 3, it is characterised in that：The micro syringe and constant injection
Device is transparent glass syringe.
5. volum-controlled type automatic dilutor according to claim 5, it is characterised in that：The micro syringe is 1000 μ L.
6. volum-controlled type automatic dilutor according to claim 5, it is characterised in that：The constant syringe is 25mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810173889.1A CN108072557B (en) | 2018-03-02 | 2018-03-02 | Constant volume type automatic dilution instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810173889.1A CN108072557B (en) | 2018-03-02 | 2018-03-02 | Constant volume type automatic dilution instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108072557A true CN108072557A (en) | 2018-05-25 |
| CN108072557B CN108072557B (en) | 2024-06-11 |
Family
ID=62155280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810173889.1A Active CN108072557B (en) | 2018-03-02 | 2018-03-02 | Constant volume type automatic dilution instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108072557B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109580321A (en) * | 2018-11-27 | 2019-04-05 | 迪瑞医疗科技股份有限公司 | A kind of fluid path air bubble eliminating device and method |
| CN111487115A (en) * | 2020-03-26 | 2020-08-04 | 江苏省计量科学研究院(江苏省能源计量数据中心) | Pneumatic solution dilution and distribution device |
| CN111811919A (en) * | 2020-07-10 | 2020-10-23 | 北京莱伯泰科仪器股份有限公司 | Tandem injection pump set structure for diluting trace liquid sample |
| CN114433265A (en) * | 2020-11-03 | 2022-05-06 | 深圳市帝迈生物技术有限公司 | Liquid feeding device and sample analyzer |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9522205D0 (en) * | 1994-11-04 | 1996-01-03 | Commissariat A I En Atomique | Fluidic module for automatic analysis device |
| JP2001343371A (en) * | 2000-06-05 | 2001-12-14 | Shimadzu Corp | Liquid chromatograph |
| CN203908855U (en) * | 2014-06-23 | 2014-10-29 | 青岛普仁仪器有限公司 | Diluting and mixing device of automatic online sample and chromatographic analysis instrument |
| CN204065036U (en) * | 2014-09-04 | 2014-12-31 | 陕西煤业化工技术开发中心有限责任公司 | A kind of pretreating device of TDI product |
| CN204116276U (en) * | 2014-09-18 | 2015-01-21 | 上海通微分析技术有限公司 | The liquid conveying system of full-automatic capillary electrophoresis apparatus |
| CN106018596A (en) * | 2016-05-17 | 2016-10-12 | 华南理工大学 | Online microwave-assisted chiral derivatization-LC/MS (liquid chromatography/mass spectrometry) analysis system and method |
| CN208026537U (en) * | 2018-03-02 | 2018-10-30 | 南京科创检测技术有限公司 | A kind of volum-controlled type automatic dilutor |
-
2018
- 2018-03-02 CN CN201810173889.1A patent/CN108072557B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9522205D0 (en) * | 1994-11-04 | 1996-01-03 | Commissariat A I En Atomique | Fluidic module for automatic analysis device |
| JP2001343371A (en) * | 2000-06-05 | 2001-12-14 | Shimadzu Corp | Liquid chromatograph |
| CN203908855U (en) * | 2014-06-23 | 2014-10-29 | 青岛普仁仪器有限公司 | Diluting and mixing device of automatic online sample and chromatographic analysis instrument |
| CN204065036U (en) * | 2014-09-04 | 2014-12-31 | 陕西煤业化工技术开发中心有限责任公司 | A kind of pretreating device of TDI product |
| CN204116276U (en) * | 2014-09-18 | 2015-01-21 | 上海通微分析技术有限公司 | The liquid conveying system of full-automatic capillary electrophoresis apparatus |
| CN106018596A (en) * | 2016-05-17 | 2016-10-12 | 华南理工大学 | Online microwave-assisted chiral derivatization-LC/MS (liquid chromatography/mass spectrometry) analysis system and method |
| CN208026537U (en) * | 2018-03-02 | 2018-10-30 | 南京科创检测技术有限公司 | A kind of volum-controlled type automatic dilutor |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109580321A (en) * | 2018-11-27 | 2019-04-05 | 迪瑞医疗科技股份有限公司 | A kind of fluid path air bubble eliminating device and method |
| CN109580321B (en) * | 2018-11-27 | 2021-05-18 | 迪瑞医疗科技股份有限公司 | Liquid path bubble removing device and method |
| CN111487115A (en) * | 2020-03-26 | 2020-08-04 | 江苏省计量科学研究院(江苏省能源计量数据中心) | Pneumatic solution dilution and distribution device |
| CN111487115B (en) * | 2020-03-26 | 2021-03-26 | 江苏省计量科学研究院(江苏省能源计量数据中心) | Pneumatic solution dilution and distribution device |
| CN111811919A (en) * | 2020-07-10 | 2020-10-23 | 北京莱伯泰科仪器股份有限公司 | Tandem injection pump set structure for diluting trace liquid sample |
| CN114433265A (en) * | 2020-11-03 | 2022-05-06 | 深圳市帝迈生物技术有限公司 | Liquid feeding device and sample analyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108072557B (en) | 2024-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108072557A (en) | A kind of volum-controlled type automatic dilutor | |
| CN102353724B (en) | On-line detection system and method of trace dissolved gas in organic oil | |
| JP2001504583A (en) | Fluid sample carbon analyzer | |
| CN208026537U (en) | A kind of volum-controlled type automatic dilutor | |
| CN109772822A (en) | A kind of stream type cell analyzer automatic washing system and its working method | |
| CN107219374A (en) | One kind analysis metering device and liquid analysis system | |
| CN104297023B (en) | A cell analyzer, a mixing device thereof and a mixing method | |
| CN211424010U (en) | Multi-range water quality analyzer flow path system | |
| CN201141864Y (en) | Automatic chemical analyzer | |
| CN108088683A (en) | Diesel engine spray nozzle clogging simulation experiment method and equipment | |
| CN105089563B (en) | The water mixing of ternary extraction well, casing medicament adding device | |
| CN209043672U (en) | A kind of atmospheric sampling absorption bottle | |
| CN207786607U (en) | A kind of evenly mixing device for test sample in analyzer reaction tank | |
| CN115616233A (en) | Online dilution device and method for flow injection analyzer | |
| CN208297540U (en) | A kind of Flow Injection Analysis device for saving reagent solution | |
| CN208902751U (en) | Quantitative sample injection and cleaning system | |
| CN206463983U (en) | A kind of liquid storage container and flow injecting system | |
| CN214668823U (en) | Full-automatic standard oil sample configuration detection system | |
| CN215877791U (en) | Liquid phase device capable of automatically proportioning and mixing | |
| CN107024570A (en) | A kind of total nitrogen, phosphorus online automatic monitor and its cleaning method | |
| CN104062169B (en) | A kind of automatic on-line micro-example dilution mixing device and mixed method | |
| CN211118782U (en) | Flow path system of water quality analyzer | |
| CN113899871A (en) | Liquid path system of water quality online monitoring and quality control instrument and use method thereof | |
| CN102632057B (en) | Cleaning device for capillary tube of automatic sampler and cleaning method | |
| CN205914473U (en) | Biochemical analyzer self - cleaning device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |