CN115616233A - Online dilution device and method for flow injection analyzer - Google Patents
Online dilution device and method for flow injection analyzer Download PDFInfo
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- CN115616233A CN115616233A CN202211416684.4A CN202211416684A CN115616233A CN 115616233 A CN115616233 A CN 115616233A CN 202211416684 A CN202211416684 A CN 202211416684A CN 115616233 A CN115616233 A CN 115616233A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
- G01N35/085—Flow Injection Analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
The invention discloses an online diluting device and method for a flow injection analyzer, wherein the online diluting device for the flow injection analyzer comprises a control unit, a sample introduction unit, a uniform mixing unit, a two-channel multi-position switching valve and a transfusion unit. The online dilution method for the flow injection analyzer of the present invention comprises the steps of: step 1: initializing the process; step 2: diluting; and step 3: in the infusion process, the infusion unit transmits the diluted sample to the flow injection analyzer for subsequent processes; and 4, step 4: and (5) cleaning. The technical scheme of the invention realizes the simultaneous and continuous operation of sample introduction, proportional dilution and sample output. The invention realizes the automatic on-line dilution of high-concentration samples, has large dilution proportion range, high accuracy and good stability, is convenient for debugging, cleaning and maintenance, can work with a flow injection analyzer simultaneously without mutual interference, meets the requirement of rapid analysis, and has good popularization significance.
Description
Technical Field
The invention relates to the technical field of flow injection analysis, in particular to an online dilution device and method for a flow injection analyzer.
Background
The flow injection analysis is a fast unsteady state analysis method, which mainly comprises the steps of carrying a certain volume of sample with a carrier flow to perform chemical reaction with a reagent in a closed pipeline to generate a mixture with certain absorbance, and detecting the mixture by a photometric detector to form a detection peak shape. Flow injection analysis is widely used in the fields of clinical chemistry, pharmaceutical chemistry, agricultural chemistry, food analysis, metallurgical analysis, environmental analysis, and the like. The existing full-automatic analysis instrument based on the flow injection analysis principle needs to draw a standard curve before detection is carried out each time, and the high-concentration sample to be tested needs to be diluted in advance.
The dilution process of the standard curve or sample is usually carried out manually, using an off-line dilution station or using a dilution device controlled by a proportional solenoid valve. The traditional manual method needs to calculate for many times, an accurate measuring device is needed to dilute the stock solution to the target concentration during operation, the requirement on operators is high, the workload is large, and errors are easy to occur; the offline dilution workstation has complicated flow and low efficiency, cannot meet the requirement of online dilution of a sample and has high cost; the diluting device based on the control of the proportional solenoid valve is not resistant to strong acid and alkali, and the valve core is easy to block due to the physical characteristics of the diluting device, so that the accuracy of a test result is low, the long-term stability is poor, and the material and maintenance cost is high.
Disclosure of Invention
The invention aims to provide an on-line diluting device and method for a flow injection analyzer, which have the advantages of flexible dilution multiple, high accuracy, good stability, convenient debugging, cleaning and maintenance of the device, simple structure and convenient use.
The invention relates to an on-line diluting device for a flow injection analyzer, which comprises a control unit, a sample introduction unit, a mixing unit, a two-channel multi-position switching valve and a transfusion unit,
the control unit comprises a sample injector and a transmission device for controlling the sample injector;
the sample injection unit comprises a three-channel multi-position switching valve, a sample injection needle, a sample container, a diluent container and a waste liquid container, wherein a first switching port of the three-channel multi-position switching valve is connected with the sample injection needle, a second switching port of the three-channel multi-position switching valve is connected with the diluent container, and a third switching port of the three-channel multi-position switching valve is connected with the waste liquid container;
the blending unit comprises a first four-channel switching valve, a current-carrying pool and a second four-channel switching valve, a main port of the two-channel multi-position switching valve is connected with the sample injector, a first switching port of the two-channel multi-position switching valve is connected with the main port of the three-channel multi-position switching valve, a second switching port of the two-channel multi-position switching valve is connected with a first switching port of the first four-channel switching valve, a second switching port of the first four-channel switching valve and a second switching port of the second four-channel switching valve are connected through pipelines, a third switching port of the first four-channel switching valve and a first switching port of the second four-channel switching valve are connected with the current-carrying pool, a fourth switching port of the first four-channel switching valve is connected with a fourth switching port of the second four-channel switching valve, and a third switching port of the second four-channel switching valve is connected with the infusion unit.
According to the online dilution device for the flow injection analyzer, the mixing unit further comprises a first solution retention ring and a second solution retention ring, the lengths of the first solution retention ring and the second solution retention ring are the same, a second switching port of the first four-channel switching valve and a second switching port of the second four-channel switching valve are connected through the second solution retention ring, and a fourth switching port of the first four-channel switching valve and a fourth switching port of the second four-channel switching valve are connected through the first solution retention ring.
According to the online diluting device for the flow injection analyzer, the infusion unit is a peristaltic pump.
The online dilution method for a flow injection analyzer of the present invention, which employs the online dilution apparatus for a flow injection analyzer of the present invention, comprises the steps of:
step 1: in the initialization process, the control unit and the infusion unit control each liquid to fill the corresponding connecting pipeline;
and 2, step: in the dilution process, the control unit controls the sample and the diluent to enter and discharge the sample and the diluent into the blending unit;
and 3, step 3: in the infusion process, the infusion unit transmits the diluted sample to the flow injection analyzer for subsequent processes;
and 4, step 4: and in the cleaning process, the control unit controls the diluent to be fed in, and the sample injector is cleaned, and then the waste liquid is discharged into a waste liquid container.
The technical scheme of the invention is suitable for flow injection analysis. The technical scheme of the invention realizes the simultaneous and continuous operation of sample introduction, proportional dilution and sample output. The invention realizes the automatic on-line dilution of high-concentration samples, has large dilution proportion range, high accuracy and good stability, is convenient for debugging, cleaning and maintenance, can work with a flow injection analyzer simultaneously without mutual interference, meets the requirement of rapid analysis, and has good popularization significance.
Drawings
Fig. 1 is a schematic view of the structure of an on-line dilution apparatus for a flow injection analyzer according to the present invention.
Detailed Description
As shown in fig. 1, the online dilution device for a flow injection analyzer of the present invention includes a control unit 1, a sample introduction unit 2, a mixing unit 3, a two-channel multi-position switching valve 4, and a fluid infusion unit 5.
The control unit 1 comprises a sample injector 7 and a transmission device 6 for controlling the sample injector, the transmission device 6 can be a motor, the sample injector 7 can be a sleeve piston rod structure for extracting and pushing liquid, and the output end of the motor is connected to a piston rod to control the sample injector to work and stop.
The sample injection unit 2 comprises a three-channel multi-position switching valve 8, a sample injection needle 9, a sample container 10, a diluent container 11 and a waste liquid container 12, wherein a first switching port B1 of the three-channel multi-position switching valve 8 is connected with the sample injection needle 9, a second switching port B2 of the three-channel multi-position switching valve is connected with the diluent container 11, and a third switching port B3 of the three-channel multi-position switching valve is connected with the waste liquid container 12;
the blending unit 3 comprises a first four-channel switching valve 13, a current carrying pool 15 and a second four-channel switching valve 17, a main port A of the two-channel multi-position switching valve 4 is connected with the sample injector 7, a first switching port A1 of the two-channel multi-position switching valve 4 is connected with a main port B of the three-channel multi-position switching valve 9, a second switching port A2 of the two-channel multi-position switching valve 4 is connected with a first switching port C1 of the first four-channel switching valve 13, a second switching port C2 of the first four-channel switching valve 13 and a second switching port D2 of the second four-channel switching valve 17 are connected through a pipeline, a third switching port C3 of the first four-channel switching valve 13 and a first switching port D1 of the second four-channel switching valve are connected with the current carrying pool 15, a fourth switching port C4 of the first four-channel switching valve is connected with a fourth switching port D4 of the second four-channel switching valve, and a third switching port D3 of the second four-channel switching valve is connected with the current carrying pool 5.
The main port a of the two-channel multi-position switching valve 4 is connected to the sleeve of the sample injector 7, so that the sample injector 7 can absorb the liquid in the sample inlet container 10 or the diluent container 11 from the main port a of the two-channel multi-position switching valve 4, and can also inject the liquid into the homogenizing unit 3 or inject the liquid from the homogenizing unit 3 into the waste liquid container 12.
According to the online dilution device for the flow injection analyzer, the blending unit 3 further comprises a first solution retention ring 14 and a second solution retention ring 16, the lengths of the first solution retention ring 14 and the second solution retention ring 16 are the same, a second switching port of the first four-channel switching valve and a second switching port of the second four-channel switching valve are connected through the second solution retention ring, and a fourth switching port of the first four-channel switching valve and a fourth switching port of the second four-channel switching valve are connected through the first solution retention ring.
The four-channel switching valve comprises a valve casing and a valve core, wherein a first switching port, a second switching port, a third switching port and a fourth switching port are arranged on the valve casing, and a first channel and a second channel are rotatably arranged in the valve core. The first channel is used for enabling the first switching port to be communicated with the second switching port or enabling the first switching port to be communicated with the fourth switching port, and the second channel is used for enabling the fourth switching port to be communicated with the third switching port or enabling the third switching port to be communicated with the second switching port.
According to the online diluting device for the flow injection analyzer, the infusion unit is a peristaltic pump.
The online dilution method for a flow injection analyzer of the present invention, which employs the online dilution apparatus for a flow injection analyzer of the present invention, comprises the steps of:
step 1: in the initialization process, the control unit and the infusion unit control each liquid to fill the corresponding connecting pipeline;
step 2: in the dilution process, the control unit controls the sample and the diluent to enter and discharge the sample and the diluent into the blending unit;
and step 3: in the infusion process, the infusion unit transmits the diluted sample to the flow injection analyzer for subsequent processes;
and 4, step 4: and in the cleaning process, the control unit controls the diluent to enter, and the waste liquid is discharged into the waste liquid container after the sample injector is cleaned.
The invention discloses an online diluting device for a flow injection analyzer, wherein an infusion unit is a single peristaltic pump or an existing peristaltic pump on the flow injection analyzer.
The invention relates to an online diluting device for a flow injection analyzer, wherein the parts are connected through pipelines which are plastic tubes, quartz tubes or glass tubes.
The invention relates to an online diluting device for a flow injection analyzer, which consists of a control unit, a sample introduction unit, a mixing unit, a two-channel multi-position switching valve and a transfusion unit. The control unit comprises a sample injector and a transmission device for controlling the sample injector and is used for controlling different liquid flow rates, flow rates and flow directions; the sample injection unit comprises a three-channel multi-position switching valve, a sample injection needle, a sample container, a diluent container and a waste liquid container; the mixing unit comprises a four-channel switching valve, a solution retaining ring and a current-carrying pool and is used for realizing the sufficient mixing and further transmission of the sample and the diluent; the infusion unit is an independent peristaltic pump or an existing peristaltic pump on the flow injection analyzer. The units and the components are communicated with each other through pipelines.
The technical scheme of the invention is suitable for flow injection analysis. The technical scheme of the invention realizes the simultaneous and continuous operation of sample introduction, proportional dilution and sample output. The invention realizes the automatic on-line dilution of high-concentration samples, has large dilution proportion range, high accuracy and good stability, is convenient for debugging, cleaning and maintenance, can work with a flow injection analyzer simultaneously without mutual interference, meets the requirement of rapid analysis, and has good popularization significance.
The working process of the on-line diluting device for the flow injection analyzer comprises the following steps:
step 1: and (5) initializing the process. The control unit and the infusion unit control each liquid to fill the corresponding connecting pipeline;
step 2: and (4) diluting. The control unit controls the sample and the diluent to enter and discharge the sample and the diluent into the blending unit;
and step 3: and (4) an infusion process. The transfusion unit transmits the diluted sample to a flow injection analyzer for subsequent processes;
and 4, step 4: and (5) cleaning. The control unit controls the diluent to enter, and the waste liquid is discharged into the waste liquid container after the sample injector is cleaned.
The technical scheme of the invention can dilute a high-concentration sample on line, can dilute the sample in any proportion within 1-1000 times by matching with the sample injectors with different specifications, has simple process and accurate result, has good stability, can realize the on-line configuration of a standard curve, can be compatible with different modules of a flow injection analyzer, has high automation degree, and meets the requirement of rapid analysis.
The invention provides an online diluting device for a flow injection analyzer, which consists of a control unit 1, a sample introduction unit 2, a blending unit 3, a two-channel multi-position switching valve 4, a transfusion unit 5 and a connecting pipeline.
The control unit 1 comprises an injector 7 and a transmission 6 for controlling the injector.
The sample injection unit 2 comprises a three-channel multi-position switching valve 8, a sample injection needle 9, a sample container 10, a diluent container 11 and a waste liquid container 12.
The blending unit 3 comprises a first four-channel switching valve 13, a first solution retention ring 14, a carrying pool 15, a second solution retention ring 16 and a second four-channel switching valve 17.
The main ports a of the two-channel multi-position switching valves 4 are directly connected with the sample injector 7 or connected through the connecting lines, the first switching ports A1 of the two-channel multi-position switching valves 4 are connected with the main ports B of the three-channel multi-position switching valves 8 through the connecting lines, and the second switching ports A2 of the two-channel multi-position switching valves 4 are connected with the first switching ports C1 of the first four-channel switching valves 13 through the connecting lines.
The infusion unit 5 is a single peristaltic pump or an existing peristaltic pump on a flow injection analyzer.
The first switching port B1 of the three-channel multi-position switching valve 8 is connected to the sample injection needle 9 through the connecting line, the second switching port B2 of the three-channel multi-position switching valve 8 is connected to the diluent container 11 through the connecting line, and the third switching port B3 of the three-channel multi-position switching valve 8 is connected to the waste liquid container 12 through the connecting line.
The second switching ports C2, D2 of the first and second four- channel switching valves 13, 17 are connected to the second solution retention ring 16 through the connection lines, the fourth switching ports C4, D4 of the first and second four- channel switching valves 13, 17 are connected to the first solution retention ring 14 through the connection lines, and the second four-channel switching valve 17 is connected to the infusion unit 5 through the connection lines.
The first solution retention ring 14 and the second solution retention ring 16 are constituted by the same length of the connection line.
The connecting pipeline is a plastic pipe, a quartz pipe or a glass pipe.
The invention also provides an online dilution method for the flow injection analyzer, which adopts the online dilution device for the flow injection analyzer, and the specific method comprises the following steps:
step 1: and (5) initializing the process. The control unit 1 and the infusion unit 5 control each liquid to fill the corresponding connecting pipeline;
step 2: and (4) diluting. The control unit 1 controls the sample and the diluent to enter and discharge the sample and the diluent into the blending unit 3;
and 3, step 3: and (4) an infusion process. The transfusion unit 5 transmits the diluted sample to a flow injection analyzer for subsequent processes;
and 4, step 4: and (5) cleaning. The control unit 1 controls the dilution liquid to be supplied, and the waste liquid is discharged into the waste liquid container 12 after the sample injector 7 is cleaned.
An experiment for testing various parameters of a water sample was conducted by using the example of FIG. 1, in which all of the diluents used were ultrapure water having a resistivity of 18.2 M.OMEGA.. Cm (25 ℃ C.). The results are shown in Table 1.
Table 1 results of the on-line dilution apparatus of the present invention for measuring different parameters in a water sample
The automatic online dilution device for the flow injection analyzer provided by the invention can be compatible with different method modules of the flow injection analyzer, can realize online configuration of a standard curve and online dilution of a high-concentration sample, has the advantages of simple process, accurate result, good stability and high automation degree, and meets the requirement of rapid analysis.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. An on-line dilution device for a flow injection analyzer is characterized by comprising a control unit, a sample introduction unit, a uniform mixing unit, a two-channel multi-position switching valve and a transfusion unit,
the control unit comprises a sample injector and a transmission device for controlling the sample injector;
the sample injection unit comprises a three-channel multi-position switching valve, a sample injection needle, a sample container, a diluent container and a waste liquid container, wherein a first switching port of the three-channel multi-position switching valve is connected with the sample injection needle, a second switching port of the three-channel multi-position switching valve is connected with the diluent container, and a third switching port of the three-channel multi-position switching valve is connected with the waste liquid container;
the blending unit comprises a first four-channel switching valve, a current-carrying pool and a second four-channel switching valve, a main port of the two-channel multi-position switching valve is connected with the sample injector, a first switching port of the two-channel multi-position switching valve is connected with the main port of the three-channel multi-position switching valve, a second switching port of the two-channel multi-position switching valve is connected with a first switching port of the first four-channel switching valve, a second switching port of the first four-channel switching valve and a second switching port of the second four-channel switching valve are connected through pipelines, a third switching port of the first four-channel switching valve and a first switching port of the second four-channel switching valve are connected with the current-carrying pool, a fourth switching port of the first four-channel switching valve is connected with a fourth switching port of the second four-channel switching valve, and a third switching port of the second four-channel switching valve is connected with the infusion unit.
2. The online dilution apparatus for a flow injection analyzer according to claim 1, wherein the mixing unit further includes a first solution retention ring and a second solution retention ring, the first solution retention ring and the second solution retention ring have the same length, the second switching port of the first four-channel switching valve and the second switching port of the second four-channel switching valve are connected through the second solution retention ring, and the fourth switching port of the first four-channel switching valve and the fourth switching port of the second four-channel switching valve are connected through the first solution retention ring.
3. The in-line dilution device for a flow injection analyzer of claim 2, wherein the infusion unit is a peristaltic pump.
4. An online dilution method for a flow injection analyzer, the method employing the online dilution apparatus for a flow injection analyzer according to any one of claims 1 to 3, the method comprising the steps of:
step 1: in the initialization process, the control unit and the infusion unit control each liquid to fill the corresponding connecting pipeline;
step 2: in the dilution process, the control unit controls the sample and the diluent to enter and discharge the sample and the diluent into the blending unit;
and 3, step 3: in the infusion process, the infusion unit transmits the diluted sample to the flow injection analyzer for subsequent processes;
and 4, step 4: and in the cleaning process, the control unit controls the diluent to enter, and the waste liquid is discharged into the waste liquid container after the sample injector is cleaned.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211416684.4A CN115616233A (en) | 2022-11-13 | 2022-11-13 | Online dilution device and method for flow injection analyzer |
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| CN202211416684.4A CN115616233A (en) | 2022-11-13 | 2022-11-13 | Online dilution device and method for flow injection analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117347457A (en) * | 2023-10-07 | 2024-01-05 | 山东谦诺生物科技有限公司 | Automatic calibration system and method for on-line detection of enzyme electrode of bioreactor |
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2022
- 2022-11-13 CN CN202211416684.4A patent/CN115616233A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117347457A (en) * | 2023-10-07 | 2024-01-05 | 山东谦诺生物科技有限公司 | Automatic calibration system and method for on-line detection of enzyme electrode of bioreactor |
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