CN111007010B - Flow path control system and method for soil biological available nitrogen and phosphorus - Google Patents
Flow path control system and method for soil biological available nitrogen and phosphorus Download PDFInfo
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- CN111007010B CN111007010B CN201911357326.9A CN201911357326A CN111007010B CN 111007010 B CN111007010 B CN 111007010B CN 201911357326 A CN201911357326 A CN 201911357326A CN 111007010 B CN111007010 B CN 111007010B
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Abstract
The invention relates to the technical field of soil biological available state element detection, in particular to a soil biological available nitrogen and phosphorus detection flow path control system and a method thereof. The chemical reaction pipeline comprises a uniform spiral ring, ninth and tenth tee joints, a chemical reaction cavity, a first peristaltic pump, a second tee joint, first and second pinch valves, a first waste liquid bottle and a micro sample injection branch; the sample detection pipeline comprises a first photoelectric liquid level switch, a second photoelectric liquid level switch, third pinch valves, fifth pinch valves, third tee joints, fifth tee joints, a second peristaltic pump, a water bath heating unit, a photoelectric detection unit and cleaning liquid; the automatic cleaning pipeline comprises sixth-eighth tee joints, sixth-eighth pinch valves, acidic solution, alkaline solution and deionized water. The invention realizes the automatic analysis of the detection of the soil biological available nitrogen and phosphorus, and improves the detection efficiency and accuracy.
Description
Technical Field
The invention relates to the technical field of soil biological available state element detection, in particular to a soil biological available state nitrogen and phosphorus flow path control detection system and a method thereof.
Background
The soil biological available nitrogen and phosphorus refer to elements which can be directly absorbed and utilized by plants and are closely related to the crop yield. Excessive biological available nitrogen and phosphorus in soil can flow into rivers, lakes and underground water through leaching or surface runoff to destroy the ecological environment of farmlands, the content of nitrogen and phosphorus elements in soil types of various areas is obtained through experiments, the content of the available state of the soil elements and the supply level of available nutrients are roughly calculated, a scientific and effective fertilization decision scheme can be made by fertilizer experts, and the damage to the farmland environment caused by excessive nutrient loss is reduced.
The traditional method for detecting nitrogen and phosphorus in soil is to extract a filtrate from a sample through a series of extraction modes, absorb the filtrate into a cuvette, add a color developing agent, conduct color comparison, and measure absorbance.
In practice, the inventors found that the above prior art has the following disadvantages:
the traditional detection method is used for detection, and the detection accuracy is low due to more external influence factors.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a system and a method for controlling and detecting a nitrogen and phosphorus flow path in an effective state of soil organisms, wherein the technical scheme is as follows:
in a first aspect, an embodiment of the present invention provides a soil biological available nitrogen and phosphorus flow path control system, which includes a chemical reaction pipeline, a sample detection pipeline and an automatic cleaning pipeline, where the chemical reaction pipeline includes a chemical reaction chamber, a ninth three-way interface, a uniform spiral ring, a tenth three-way interface, a second pinch valve, a second three-way interface, a first peristaltic pump, a first three-way interface and a first pinch valve, which are connected in sequence, one end of the ninth three-way interface is connected to one end of the uniform spiral ring, the other end of the ninth three-way interface is connected to a micro sample introduction branch, and the micro sample introduction branch is used for injecting a leached soil sample and a color reagent into the chemical reaction chamber; the other end of the uniform spiral ring is connected to a first peristaltic pump through the tenth three-way connector, a second pinch valve and a second three-way connector, and the first peristaltic pump is connected with a first waste liquid bottle through the first three-way connector and the first pinch valve in sequence; the sample detection pipeline comprises a first photoelectric liquid level switch, a third pinch valve, a third tee joint, a second photoelectric liquid level switch, a fourth pinch valve, a fourth tee joint, a second peristaltic pump, a water bath heating unit and a photoelectric detection unit which are connected in sequence, and the other end of the fourth tee joint is connected with an air channel; the first photoelectric liquid level switch is connected between the second three-way connector and the third pinch valve, and the other end of the third pinch valve is connected with the cleaning liquid through a fifth three-way connector and a fifth pinch valve in sequence; the automatic cleaning pipeline is used for cleaning the chemical reaction pipeline and comprises a sixth tee joint and a sixth pinch valve which are sequentially connected with the first peristaltic pump; one end of the sixth pinch valve is connected with the acidic solution, and the other end of the sixth pinch valve is connected with a seventh pinch valve through a seventh tee joint; one end of the seventh pinch valve is connected with the alkaline solution, and the other end of the seventh pinch valve is connected with the eighth pinch valve through an eighth tee joint; one end of the eighth pinch valve is connected with the deionized water, and the other end of the eighth pinch valve is connected with the air channel.
Further, the micro sample injection branch comprises a micro injection pump, an electromagnetic switching valve, a liquid storage ring, a multi-channel reagent switching valve and a multi-channel sample switching valve which are sequentially connected, and deionized water connected with another channel of the electromagnetic switching valve, wherein the liquid storage ring is connected with one end of the multi-channel reagent switching valve through a liquid input channel and a first three-way interface connection.
In a second aspect, an embodiment of the present invention provides a method for controlling a nitrogen and phosphorus flow path in a soil biological available state, where the method includes the following steps:
closing a sample detection pipeline by using a third pinch valve, switching a second pinch valve which is connected in series into a chemical reaction pipeline to a pipeline connected with a second three-way interface, and switching a first pinch valve which is connected with a first peristaltic pump in the chemical reaction pipeline to a waste liquid pipeline, injecting the extracted soil sample and a color reagent into a chemical reaction cavity in the chemical reaction pipeline through a micro sample injection branch, wherein when one color reagent is injected, the first peristaltic pump rotates reversely to make the solution in the chemical reaction cavity flow into a uniform spiral ring, then the first pinch valve is switched to an air pipeline, the first peristaltic pump rotates positively, and the solution in the uniform spiral ring is pumped into the chemical reaction cavity again; in the process of positive and negative rotation of the first peristaltic pump, a solution flows between the chemical reaction cavity and the uniform spiral ring, so that the extracted soil sample and the color developing reagent are fully reacted, and finally a test sample solution is obtained;
opening a sample detection pipeline by using the third pinch valve, enabling the test sample solution to enter the sample detection pipeline through a second peristaltic pump, and switching a fourth pinch valve connected in series with the sample detection pipeline to an air channel at intervals, so that the test sample solution is uniformly divided into a plurality of sections by an air section; and the test sample solution enters the photoelectric detection unit for detection through the water bath heating unit under the action of the second peristaltic pump.
Further, a first photoelectric liquid level switch is connected between the second three-way connector and a third pinch valve, and a second photoelectric liquid level switch is connected between the third three-way connector connected with the third pinch valve and a fourth pinch valve; when the test sample solution is detected, the first photoelectric liquid level switch is turned on, and the test sample solution enters the sample detection pipeline; when the test sample solution completely enters the sample detection pipeline, the second photoelectric liquid level switch is changed from bright to dark.
Further, after the second photoelectric liquid level switch is changed from bright to dark, the control method further comprises the following steps:
the third pinch valve is switched to be connected with a fifth three-way connector, and the fifth pinch valve connected with the fifth three-way connector is switched to a corresponding cleaning liquid pipeline; and the second peristaltic pump pumps the cleaning solution to clean the sample detection pipeline.
Further, the detection method also comprises a cleaning step of the chemical reaction pipeline:
according to the acid-base property of the test sample solution, the sample detection flow path is closed through the third pinch valve, the first pinch valve is switched to the waste liquid pipeline, and the chemical reaction cavity and the uniform spiral ring are cleaned by using an alkaline solution or an acidic solution connected with the first peristaltic pump; then, the deionized water connected with the first peristaltic pump is switched to be cleaned, and the second pinch valve is switched to an air channel to fill the chemical reaction cavity with the deionized water; and then the second pinch valve is switched to a pipeline connected with the second three-way connector, and the first peristaltic pump rotates at a low speed, so that all the solution in the chemical reaction cavity is pumped into the waste liquid pipeline.
The invention has the following beneficial effects:
the embodiment of the invention provides a soil biological available nitrogen and phosphorus flow path control detection system, wherein a soil sample and required reagents enter a chemical reaction cavity in sequence, and after the sample is injected into the chemical reaction cavity, each chemical reaction reagent is injected, and a corresponding pinch valve is automatically switched to a sample and reagent chemical reaction pipeline; under the action of the first peristaltic pump, the leached soil sample and the reagent circularly flow between the chemical reaction cavity and the uniform spiral ring, and finally a test sample solution is obtained, so that the chemical reaction efficiency of the soil biological available nitrogen and phosphorus sample and the reagent is effectively improved; after the sample and the reagent are completely injected and fully mixed to generate chemical reaction, the chemical reaction pipeline is closed by the pinch valve, the sample detection pipeline is switched to, the test sample solution passes through the water bath heating unit, the detention time of the reaction solution can be increased and decreased by the spiral pipe in the water bath unit, and finally the reaction solution is pumped into the photoelectric detection unit for testing. Before flowing into the photoelectric detection unit for testing, the pinch valve is continuously switched to an air channel, and the chemical reaction solution is uniformly divided into a plurality of sections for detection, so that the detection accuracy of the available nitrogen and phosphorus in the soil organisms is effectively improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions and advantages of the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of a soil bio-available nitrogen and phosphorus detection system according to another embodiment of the present invention;
FIG. 2 is a diagram showing the response of a photodetector of a soil bio-available nitrogen and phosphorus detection system according to an embodiment of the present invention;
FIG. 3 is a diagram illustrating the results of a soil bio-available ammonium nitrogen test according to an embodiment of the present invention;
FIG. 4 is a graph showing the results of a soil bioavailable state of ammonium phosphorus test provided by one embodiment of the present invention.
In the figure: 100-chemical reaction pipeline, 200-sample detection pipeline, 300-automatic cleaning pipeline, 101-chemical reaction cavity, 102-uniform spiral ring, 103-micro sample introduction branch, 104-first peristaltic pump, 105-second three-way interface, 106-second pinch valve, 1061-second pinch valve air channel, 107-ninth three-way interface, 108-first three-way interface, 109-first pinch valve, 1091-first pinch valve air channel, 110-tenth three-way interface, 201-first photoelectric liquid level switch, 202-third three-way interface, 203-third pinch valve, 204-second photoelectric liquid level switch, 205-fourth three-way interface, 206-fourth pinch valve, 2061-fourth pinch valve, 207-second peristaltic pump, 208-water bath heating unit, 209-photoelectric detection unit, 301-sixth three-way interface, 302-sixth pinch valve, 303-seventh three-way interface, 304-seventh pinch valve, 305-eighth three-way interface, 306-eighth pinch valve, 3061-eighth pinch valve air channel, 307-acidic solution, 308-alkaline solution, 309-deionized water, 401-fifth three-way interface, 402-fifth pinch valve, 403-first cleaning solution, 404-second cleaning solution, 1031-micro injection pump, 1032-electromagnetic switching valve, 1033-liquid storage ring, 1034-multi-channel reagent switching valve, 10341-reagent switching valve air channel, 1035-multi-channel sample switching valve, 10351-sample switching valve air channel, 1036-second deionized water, 1037-reagent bottle, 1038-sample bottle, 11-first waste liquid bottle, 12-second waste liquid bottle, 13-third waste liquid bottle and 14-fourth waste liquid bottle.
Detailed Description
In order to further illustrate the technical means and effects of the present invention for achieving the predetermined purpose, the following detailed description of the system and method for detecting available nitrogen and phosphorus in soil organisms according to the present invention, the detailed implementation, structure, features and effects thereof are provided in the accompanying drawings and preferred embodiments. In the following description, different "one embodiment" or "another embodiment" refers to not necessarily the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
It will be understood that when an element is referred to as being "disposed" or "connected" to another element, it can be directly on the other element or intervening elements may also be present. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The concrete scheme of the soil biological available nitrogen and phosphorus detection system and the method thereof provided by the invention is specifically described below by combining the attached drawings.
Referring to fig. 1, a block diagram of a soil bio-available nitrogen and phosphorus detection system according to an embodiment of the present invention is shown, the detection system includes a chemical reaction pipeline 100, a sample detection pipeline 200, and an automatic cleaning pipeline 300.
Specifically, the chemical reaction pipeline 100 includes a uniform spiral ring 102, a ninth three-way interface 107, a chemical reaction chamber 101, a first peristaltic pump 104, a second three-way interface 105, a second pinch valve 106, a tenth three-way interface 110, a first three-way interface 108, a first pinch valve 109, and a first waste liquid bottle 11. The micro sample feeding branch 103 and the uniform spiral ring 102 are connected to one end of the chemical reaction chamber 101, the other end of the chemical reaction chamber 101, which is far away from the uniform spiral ring 102, is connected to the first peristaltic pump 104, one end of the first peristaltic pump 104, which is far away from the chemical reaction chamber 101, is connected to the second pinch valve 106 through the second three-way interface 105, one end of the second pinch valve 106, which is far away from the first peristaltic pump 104, is connected to the thirteenth interface 110, and the tenth three-way interface 110 is bridged between the uniform spiral ring 102 and the sample detection pipeline 200. Wherein, the micro sample introduction branch 103 is used for adding a soil sample solution and a reagent into the chemical reaction chamber 101.
Specifically, the sample detection pipeline 200 includes a first photoelectric liquid level switch 201, a third pinch valve 203, a third three-way interface 202, a second photoelectric liquid level switch 204, a fourth pinch valve 206, a fourth three-way interface 205, a second peristaltic pump 207, a water bath heating unit 208, and a photoelectric detection unit 209. The first photoelectric liquid level switch 201 is connected across the third pinch valve 203 and the thirteenth connection port 110, one end of the third pinch valve 203, which is far away from the first photoelectric liquid level switch 201, is connected with the fourth pinch valve 206 through the second photoelectric liquid level switch 204, one end of the fourth pinch valve 206, which is far away from the second photoelectric liquid level switch 204, is connected with the fourth three-way connection 205, the other end of the fourth pinch valve 206 is connected with air through a fourth pinch valve air passage 2061, and one end of the fourth three-way connection 205, which is far away from the fourth pinch valve 206, is sequentially connected with the water bath heating unit 208 and the photoelectric detection unit 209. The sample detection pipeline 200 further comprises a cleaning solution branch for cleaning the sample detection pipeline 200, wherein the cleaning solution branch comprises a fifth three-way interface 401 connected with the third pinch valve 203, a fifth pinch valve 402 connected with the fifth three-way interface 401, and a first cleaning solution 403 and a second cleaning solution 404 respectively connected with the fifth pinch valve 402.
Specifically, the automatic cleaning flow path includes a sixth three-way connection 301, a sixth pinch valve 302, a seventh three-way connection 303, a seventh pinch valve 304, an eighth three-way connection 305, and an eighth pinch valve 306. One end of the sixth three-way connector 301 is connected with the first peristaltic pump 104, the other two ends of the sixth three-way connector 301, which are away from the first peristaltic pump 104, are connected with the sixth pinch valve 302, wherein one end of the sixth pinch valve 302 is connected with the acidic solution 307, and the other end is connected with the seventh pinch valve 304 through the seventh three-way connector 303; one end of the seventh pinch valve 304 is connected with the alkaline solution 308, and the other end is connected with the eighth pinch valve 306 through an eighth tee joint 305; one end of the eighth pinch valve 306 is connected to the deionized water 309 and the other end is connected to air via an eighth pinch valve air passage 3061.
After the test sample solution completely enters the sample detection pipeline 200, the third pinch valve 203 is switched to the first and second cleaning solutions communicated with the fifth pinch valve 402, the cleaning solution enters the sample detection pipeline 200, the cleaning solution cleans the water bath heating unit 208 and the photoelectric detection unit 209 immediately following the test sample, and meanwhile, according to the acid-base property of the test sample solution, the sixth or seventh pinch valve 304 in the automatic cleaning flow path is firstly switched to the acidic or alkaline cleaning solution to clean the chemical reaction chamber 101 and the uniform spiral ring 102, and then is cleaned by the deionized water 309.
After each reagent required for color development is added, the third pinch valve 203 closes the channel connected to the tenth three-way connection 110, the sample detection line 200 is closed by the third pinch valve 203, the second pinch valve 106 connected in series to the chemical reaction line 100 is switched to the channel connected to the tenth three-way connection 110, and the first pinch valve 109 connected to the first peristaltic pump 104 in the chemical reaction line 100 is switched to the waste liquid line connected to the first waste liquid bottle 11. When samples and reagents are mixed, under the action of positive and negative rotation of a first peristaltic pump 104 in a chemical reaction pipeline 100, leaching soil samples and color reagents enter a chemical reaction cavity 101 through a micro sample introduction branch 103 to obtain corresponding solutions; meanwhile, under the action of the forward and reverse rotation of the first peristaltic pump 104, the solution circularly flows in the chemical reaction chamber 101 and between the uniform spiral rings 102, and a test sample solution is obtained. Specifically, when each color reagent is injected, the first peristaltic pump 104 rotates reversely, so that the solution in the chemical reaction chamber 101 flows into the uniform spiral ring 102, and is mixed in the spiral flowing process; and then the first pinch valve 109 is switched to a first pinch valve air channel 1091, the first peristaltic pump 104 rotates forwards, the solution in the uniform spiral ring 102 is pumped into the chemical reaction chamber 101 again, and in the process of rotating forwards and backwards of the first peristaltic pump 104, the solution flows between the chemical reaction chamber 101 and the uniform spiral ring 102, so that the extracted soil sample and the chromogenic reagent are fully reacted, and finally the test sample solution is obtained.
When it is necessary to inject a test sample into the sample detection line 200, the sample detection line 200 is opened by the third pinch valve 203, the second pinch valve 106 is switched to the second pinch valve air passage 1061, the third pinch valve 203 is switched to a passage connected to the thirteenth connection port 110, and the fourth pinch valve 206 is switched to a passage connected to the third pinch valve 203. Under the action of the second peristaltic pump, the test sample solution enters the sample detection pipeline 200 along the tenth three-way connector 110 through the third pinch valve 203, and the photoelectric liquid level switch judges the start-stop state of the chemical reaction solution pumped into the sample detection pipeline 200. Under the action of forward rotation of the second peristaltic pump 207, a test sample solution enters the sample detection pipeline 200, the first photoelectric liquid level switch 201 is turned on, and the test sample solution starts to enter the sample detection pipeline 200; the fourth pinch valve 206 connected in series in the sample detection pipeline 200 is switched to the fourth pinch valve air passage 2061 communicated with the air at intervals, so that the test sample solution is uniformly divided into a plurality of sections by the air section, and the multiple measurements of the same detection sample solution are realized while the solution consumption is reduced. The test sample solution is then passed through a water bath heating unit 208, the coils of which 208 provide the appropriate solution residence time to provide the optimal temperature conditions for the chemical reaction. And finally, the test sample solution enters a photoelectric detection unit 209 for detection, and the content of nitrogen and phosphorus in the soil biological effective state is displayed. When the test sample solution completely enters the sample detection pipeline 200, the second photoelectric liquid level switch 204 is changed from light to dark. By the method for enabling the test sample solution to be uniformly divided into multiple sections by the air section and enter the photoelectric detection unit for detection, the aim of measuring the same soil biological available nitrogen and phosphorus test sample solution in a divided mode for multiple times is achieved, and the test accuracy can be improved.
In summary, the embodiment of the present invention provides a soil biological available nitrogen and phosphorus flow path control detection system, where the extracted soil sample and the color reagent sequentially enter the chemical reaction chamber, and after the extracted soil sample is injected into the chemical reaction chamber, each time one color reagent is injected, the corresponding pinch valve is switched to the chemical reaction pipeline; under the action of the first peristaltic pump, the leaching soil sample and the color reagent are pumped out or pumped into the chemical reaction cavity and the uniform spiral ring circularly flow process, so that the leaching soil sample and the color reagent are uniformly mixed to form a test sample solution, and the chemical reaction efficiency of the soil biological effective nitrogen and phosphorus sample and the reagent is improved; in the process of detecting the test sample solution, the pinch valves are switched to the air channels at intervals, so that the test sample solution is uniformly divided into a plurality of sections by the air sections, and the test values are averaged for a plurality of times, thereby effectively improving the accuracy of detecting the available nitrogen and phosphorus in the soil organisms.
Preferably, referring to fig. 1 again, the micro sample feeding branch 103 includes a micro syringe pump 1031, an electromagnetic switching valve 1032, a reservoir ring 1033, a multi-channel reagent switching valve 1034 and a multi-channel sample switching valve 1035, the micro syringe pump 1031 is connected to the second deionized water 1036 and the reservoir ring 1033 through the electromagnetic switching valve 1032, the other end of the reservoir ring 1033 is connected to the chemical reaction chamber 101 and the multi-channel reagent switching valve 1034, and the multi-channel reagent switching valve 1034 is connected to the multi-channel sample switching valve 1035 through a common channel. Specifically, the multi-channel reagent switching valve 1034 includes a plurality of reagent channels connected to different reagent bottles 1037, a reagent switching valve air channel 1034, and a waste channel connected to a third waste bottle. The multi-channel sample switching valve 1035 comprises a plurality of sample channels connecting different sample vials 1038, a sample switching valve air channel 10351, and a waste channel connecting a fourth waste vial 14. Specifically, in this embodiment, the multi-channel reagent switching valve 1034 is sixteen channels and the multi-channel sample switching valve 1035 is ten channels. The electromagnetic switching valve 1032 is a two-channel electromagnetic switching valve 1032.
In use, deionization is firstly carried out, and then extraction of a leached soil sample and extraction of a color developing reagent required for color development are carried out.
Wherein the deionization step comprises: first extraction of deionized water 309: the micro syringe pump 1031 pumps the second deionized water 1036 by switching to the deionized water 309 channel using the electromagnetic switching valve 1032. Deionized water 309 is then filled in the microinjection branch 103: the solenoid switching valve 1032 is switched to the line connected to the reservoir ring 1033, then the multi-channel reagent switching valve 1034 connected to the other end of the reservoir ring 1033 is switched to the common channel connected to the multi-channel sample switching valve 1035, and the multi-channel sample switching valve 1035 is switched to the waste channel, so that the channels between the reservoir ring 1033, the multi-channel reagent switching valve 1034, the multi-channel sample switching valve 1035, and the waste liquid bottle are opened, then the deionized water 309 is injected into the reservoir ring 1033 by the micro syringe pump 1031, the deionized water 309 passes through the multi-channel reagent switching valve 1034 and the multi-channel sample switching valve 1035 connected by the common channel, and the above operations are repeated, so that the reservoir ring 1033 is completely filled with the deionized water 309, and the deionized water 309 finally flows into the waste channel.
The step of extracting the sample comprises: firstly, air isolation is carried out: multi-channel sample switching valve 1035 switches to sample switching valve air channel 10351, drawing in a quantity of air that can isolate soil bio-available nitrogen phosphorus test leach soil samples from deionized water 309. Then, a sample is extracted: the multi-channel sample switching valve 1035 switches to a sample channel, and extracts the leaching soil sample solution into the reservoir ring 1033; then the multi-channel reagent switching valve 1034 switches to the waste liquid channel, so that a small amount of leaching soil enters the waste liquid channel; the multi-channel reagent switching valve 1034 then switches to a liquid input channel connected to the chemical reaction chamber 101, so that the leached soil sample enters the chemical reaction chamber 101; then the multi-channel reagent switching valve 1034 switches to the reagent switching valve air channel 1034, drawing in a certain amount of air; the multi-channel reagent switching valve 1034 switches to a liquid input channel so that the remaining sample solution is entirely introduced into the chemical reaction chamber 101. And finally, processing sample and reagent branches: after the leached soil sample is injected into the chemical reaction chamber 101, the multi-channel reagent switching valve 1034 switches to a common channel connected to the multi-channel sample switching valve 1035, the multi-channel sample switching valve 1035 switches to a waste channel, the electromagnetic switching valve 1032 switches to a deionized water 309 channel, and a second deionized water 1036 is extracted; the solenoid switching valve 1032 then switches to the reservoir ring 1033 channel to inject all of the remaining leached soil sample into the waste channel. And repeatedly extracting sufficient soil biological available nitrogen and phosphorus to detect the leached soil sample.
The step of extracting the color reagent comprises: firstly, air isolation is carried out: the multi-channel reagent switching valve 1034 switches to an air channel, drawing in a volume of air, isolating the deionized water 309 from the reagents. Then the reagent is extracted: the multi-channel reagent switching valve 1034 switches to the corresponding reagent channels in sequence according to the addition sequence of the reagents required for color development, extracts the required reagents, and after extracting the reagents, the multi-channel reagent switching valve 1034 switches to the waste liquid channel, so that a small amount of reagents enter the waste liquid channel; the multi-channel reagent switching valve 1034 then switches to the liquid input channel to inject the reagent into the chemical reaction chamber 101. The multi-channel reagent switching valve 1034 then switches to the air channel again, drawing in a quantity of air into the reservoir ring 1033. The multi-channel reagent switching valve 1034 then switches to the liquid input channel so that the reagents in the channels are all injected into the chemical reaction chamber 101. And finally, processing the trace sample injection branch 103: the solenoid switching valve 1032 switches to the channel of deionized water 309 to extract a second deionized water 1036. The solenoid switching valve 1032 then switches to the reservoir ring 1033 channel and the multi-channel reagent switching valve 1034 switches to the waste channel, allowing the excess reagent to be injected into the waste bottle. When the extracted reagent is not enough, repeating the steps, and extracting sufficient quantity of the reagent required by the soil biological effective nitrogen and phosphorus detection sample.
In the embodiment, the first cleaning solution 403 and the second cleaning solution 404 connected to the fifth pinch valve 402 are the SDS cleaning solution and the Brj cleaning solution in the second cleaning bottle, respectively.
Specifically, in this embodiment, those skilled in the art know that the photoelectric detection unit 209 at least includes a micro-flow cuvette and a photodetector, and the photoelectric detection unit 209 is used for detecting nitrogen and phosphorus elements in the test sample solution, and the photoelectric detection unit 209 is not the invention of the present invention, and therefore, the description thereof is omitted.
In addition, the photodetection unit 209 is provided with a waste liquid channel connected to the second waste liquid bottle 12.
The detection system is cleaned, including cleaning the sample detection line 200, cleaning the chemical reaction line 100, and treating with deionized water 309.
Cleaning the sample detection line 200: when the second photoelectric liquid level switch 204 is changed from bright to dark, the third pinch valve 203 is switched to a channel connected with the fifth pinch valve 402 through the fifth three-way interface, the fifth pinch valve 402 is switched to a corresponding cleaning liquid pipeline, and the second peristaltic pump 207 pumps the cleaning liquid to clean the sample detection pipeline 200. Under the action of the second peristaltic pump 207, the cleaning solution enters the sample detection pipeline 200 next to the chemical reaction solution, and the cleaning of the water bath heating unit 208 and the photoelectric detection unit 209 in the sample detection pipeline 200 is started.
Cleaning the chemical reaction pipeline 100: when the second photoelectric liquid level switch 204 is changed from bright to dark, the sample detection flow path is closed through the third pinch valve according to the acid-base property of the test sample solution, the first pinch valve 109 is switched to a waste liquid pipeline, and the chemical reaction chamber 101 and the uniform spiral ring 102 are cleaned by using the alkaline solution or the acidic solution connected with the first peristaltic pump 101. When the test sample solution is an acidic solution 307, the seventh pinch valve 304 is switched to the alkali solution channel, the sixth pinch valve 302 is switched to the channel connected with the seventh pinch valve 304, the first peristaltic pump 104 is reversed, and the alkali solution 308 is pumped to clean the test sample solution; when the test sample solution is the alkaline solution 308, the sixth pinch is to the acidic wash channel, which is cleaned by drawing the acidic solution 307.
And (3) deionized water 309 treatment, wherein the sixth pinch valve 302 is switched to be connected with a seventh pinch valve 304, the seventh pinch valve 304 is switched to be connected with an eighth pinch valve 306, the eighth pinch valve 306 is switched to be connected with the deionized water 309 connected with the first peristaltic pump for cleaning, and the first peristaltic pump 104 is reversed to clean the chemical reaction pipeline 100. Finally, the second pinch valve 106 is switched to the second pinch valve air channel 1061, so that the chemical reaction chamber 101 is filled with deionized water, the eighth pinch valve 306 is switched to the eighth pinch valve air channel 3061 again, the second pinch valve 106 is switched to the channel connected with the tenth three-way connector 110, the first peristaltic pump 104 rotates at a low speed, the first pinch valve 109 is switched to the waste liquid channel, so that the solution in the chemical reaction chamber is completely pumped into the waste liquid pipeline, and the cleaning of the chemical reaction chamber 101 and the uniform spiral ring 102 is completed.
Referring to fig. 3, a response diagram of a photodetector of a soil bio-available nitrogen phosphorus detection system according to an embodiment of the present invention is shown. Firstly, blank solution enters a photoelectric detection unit, a system records an initial light intensity value of the blank solution, and a large bubble is divided before a test sample solution enters the photoelectric detection unit. When the test sample solution enters the photoelectric detection unit, the test light intensity value rapidly drops and is then stable, the test sample solution is divided into a plurality of sections by small bubbles for measurement, the average value of the test sample solution is taken, after the test of the test sample solution is completed, a large bubble is followed, the blank solution enters the photoelectric detection unit, an ascending stage exists when the blank solution enters the photoelectric detection unit, and then the blank solution is stable for testing the next soil sample solution.
Referring to fig. 3 and 4, when the optical path length of the photoelectric detection unit is 20mm, the effective measurement ranges of the ammonium nitrogen and the available phosphorus in the soil are 0-2.6 mg/L and 0-2.6 mg/L, respectively, and the optimal linear range test charts of the ammonium nitrogen and the available phosphorus in the soil are shown in fig. 3 and 4.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (2)
1. The system is characterized by comprising a chemical reaction pipeline, a sample detection pipeline and an automatic cleaning pipeline, wherein the chemical reaction pipeline comprises a chemical reaction cavity, a ninth tee joint, a uniform spiral ring, a tenth tee joint, a second pinch valve, a second tee joint, a first peristaltic pump, a first tee joint and a first pinch valve which are sequentially connected, one end of the ninth tee joint is connected with one end of the uniform spiral ring, the other end of the ninth tee joint is connected with a micro sample introduction branch, and the micro sample introduction branch is used for injecting a leached soil sample and a color reagent into the chemical reaction cavity; the other end of the uniform spiral ring is connected to a first peristaltic pump through the tenth three-way interface, a second pinch valve and a second three-way interface, and the first peristaltic pump is connected with a first waste liquid bottle through a first three-way interface and a first pinch valve in sequence; the sample detection pipeline comprises a first photoelectric liquid level switch, a third pinch valve, a third tee joint, a second photoelectric liquid level switch, a fourth pinch valve, a fourth tee joint, a second peristaltic pump, a water bath heating unit and a photoelectric detection unit which are connected in sequence, and the other end of the fourth tee joint is connected with an air channel; the first photoelectric liquid level switch is connected between the tenth tee joint and the third pinch valve, and the other end of the third pinch valve is connected with the cleaning liquid through a fifth tee joint and a fifth pinch valve in sequence; the automatic cleaning pipeline is used for cleaning the chemical reaction pipeline and comprises a sixth tee joint and a sixth pinch valve which are sequentially connected with the first peristaltic pump; one end of the sixth pinch valve is connected with the acidic solution, and the other end of the sixth pinch valve is connected with a seventh pinch valve through a seventh tee joint; one end of the seventh pinch valve is connected with the alkaline solution, and the other end of the seventh pinch valve is connected with the eighth pinch valve through an eighth tee joint; one end of the eighth pinch valve is connected with the deionized water, and the other end of the eighth pinch valve is connected with the air channel;
the micro sample injection branch comprises a micro injection pump, an electromagnetic switching valve, a liquid storage ring, a multi-channel reagent switching valve, a multi-channel sample switching valve and deionized water connected with the other channel of the electromagnetic switching valve, wherein the micro sample injection branch is sequentially connected with the micro injection pump, the electromagnetic switching valve, the liquid storage ring, the multi-channel reagent switching valve and the multi-channel sample switching valve;
the method for applying the soil bio-available ammonia phosphorus flow path control system comprises the following steps:
closing a sample detection pipeline by using a third pinch valve, switching a second pinch valve which is connected into a chemical reaction pipeline in series to be connected with a thirteenth connection port and switching a first pinch valve which is connected with a first peristaltic pump in the chemical reaction pipeline to be a waste liquid pipeline, injecting the extracted soil sample and a chromogenic reagent into a chemical reaction cavity in the chemical reaction pipeline through a micro sample injection branch, wherein each chromogenic reagent is injected, the first peristaltic pump reversely rotates to enable a solution in the chemical reaction cavity to flow into a uniform spiral ring, then the first pinch valve is switched to an air pipeline, the first peristaltic pump rotates forwards, and the solution in the uniform spiral ring is pumped into the chemical reaction cavity again; in the process of positive and negative rotation of the first peristaltic pump, a solution flows between the chemical reaction cavity and the uniform spiral ring, so that the extracted soil sample and the color developing reagent are fully reacted, and finally a test sample solution is obtained;
opening a sample detection pipeline by using the third pinch valve, enabling the test sample solution to enter the sample detection pipeline through a second peristaltic pump, and switching a fourth pinch valve connected in series with the sample detection pipeline to an air channel at intervals, so that the test sample solution is uniformly divided into a plurality of sections by an air section; the test sample solution passes through the water bath heating unit and enters the photoelectric detection unit for detection under the action of the second peristaltic pump;
a first photoelectric liquid level switch is connected between the tenth three-way connector and the third pinch valve, and a second photoelectric liquid level switch is connected between the third three-way connector and the fourth pinch valve; when the test sample solution is detected, the first photoelectric liquid level switch is turned on, and the test sample solution enters the sample detection pipeline; when the test sample solution completely enters the sample detection pipeline, the second photoelectric liquid level switch is changed from bright to dark;
after the second photoelectric liquid level switch is dimmed by light, the control method further comprises the following steps:
the third pinch valve is switched to be connected with a fifth three-way connector, and the fifth pinch valve connected with the fifth three-way connector is switched to a corresponding cleaning liquid pipeline; and the second peristaltic pump pumps the cleaning solution to clean the sample detection pipeline.
2. The soil bio-available nitrogen phosphorus flow path control system of claim 1, wherein the detection method further comprises a step of cleaning the chemical reaction line:
according to the acid-base property of the test sample solution, the sample detection flow path is closed through the third pinch valve, the first pinch valve is switched to the waste liquid pipeline, and the chemical reaction cavity and the uniform spiral ring are cleaned by using an alkaline solution or an acidic solution connected with the first peristaltic pump; then, the deionized water connected with the first peristaltic pump is switched to be cleaned, and the second pinch valve is switched to an air channel to fill the chemical reaction cavity with the deionized water; and then the second pinch valve is switched to a pipeline connected with the thirteenth through joint, and the first peristaltic pump rotates at a low speed, so that all the solution in the chemical reaction cavity is pumped into the waste liquid pipeline.
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| CN114397172A (en) * | 2021-12-17 | 2022-04-26 | 北京市农林科学院信息技术研究中心 | Portable soil available phosphorus content detection device and detection method |
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| DE29517698U1 (en) * | 1995-07-29 | 1996-01-18 | Gottfried Bischoff Gmbh & Co | Flue gas desulfurization plant |
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