CN203786079U - Full-automatic high-precision capillary tube electrophoresis apparatus - Google Patents

Full-automatic high-precision capillary tube electrophoresis apparatus Download PDF

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Publication number
CN203786079U
CN203786079U CN201420131764.XU CN201420131764U CN203786079U CN 203786079 U CN203786079 U CN 203786079U CN 201420131764 U CN201420131764 U CN 201420131764U CN 203786079 U CN203786079 U CN 203786079U
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automatic
port
sampling
bottle
electrophoresis
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阎超
姚冬
张琳
李静
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TONGWEI ANALYSIS TECHNOLOGY Co Ltd SHANGHAI
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TONGWEI ANALYSIS TECHNOLOGY Co Ltd SHANGHAI
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Abstract

The utility model relates to a full-automatic high-precision capillary tube electrophoresis apparatus comprising an electrophoresis system, a sample introduction flow path and an automatic sampling flow path, wherein the sample introduction flow path comprises a shunt waste fluid bottle, a four-way micro-sample introduction valve and a buffer fluid injection pump connected with a four-way connector; the four-way micro-sample introduction valve is connected with an electrophoresis separation capillary tube and carries out quantitative sample introduction on the electrophoresis separation capillary tube of the electrophoresis system; the automatic sampling flow path comprises a sampling probe, a sample disc, a rinsing tank, reagent bottles, a buffer pipe, a six-end fluid distributor and an injector pump; a three-end distribution valve of the injector pump is respectively connected with a rinsing fluid bottle, an automatic sampler waste fluid bottle and the buffer pipe; a fixed opening of the six-end fluid distributor is connected with the buffer pipe; six distribution openings alternately connected with the fixed opening are respectively connected with the sampling probe, the four-way connector, the four-way micro-sample introduction valve and three reagent bottles; the sampling probe is switched to be inserted into the rinsing tank or the sample disc; the automatic sampler waste fluid bottle is connected with the four-way micro-sample introduction valve. The full-automatic high-precision capillary tube electrophoresis apparatus is high in sample introduction speed, high in accuracy, good in reproducibility and can be widely used in automatic capillary tube electrophoresis analysis of different matters.

Description

Full-automatic high precision capillary electrophoresis apparatus
Technical field
The utility model relates to the Capillary Electrophoresis of material, is specifically related to a kind of full-automatic high precision capillary electrophoresis apparatus, belongs to analysis and testing technology field.
Background technology
Capillary Electrophoresis starts from the eighties in 20th century.When analyzing, buffer solution will be full of in kapillary, kapillary two ends apply high-tension electricity (general 0 arrive+30kV or arrive-30kV), electroosmotic flow in kapillary makes solution flow to detector one end, also drives all substances in kapillary to flow to detector one end.Material in solution is because its electrophoretic mobility is different, and institute is electrically charged different from the ratio (specific charge) of its molecular weight, so arrives the speed difference of kapillary terminal detector, thereby obtains separation and detection.
Capillary electrophoresis system is exactly to utilize charge species analyzed in kapillary different principle of rate travel under electric field action, reach separated object, its critical piece comprises liquid container, electrode, high-voltage power supply supply, detecting device and data output and the treating apparatus at an elongate tubular kapillary, kapillary two ends.
Conventional input mode capillaceous has two kinds: fluid mechanics and electromigration injection.Fluid mechanics sample introduction can be by siphon, in sample introduction end pressurization or the detector end method such as find time, realize, and electromigration injection is to apply voltage, by electric field action, relies on electromigration and (or) the electroosmotic flow of sample ions that sample is injected to kapillary.There is following shortcoming in above-mentioned two kinds of input modes: because two kinds of methods all adopt the mode of " dipping ", complete sample introduction 1., both kapillary was dipped in sample bottle, exert pressure or voltage sample introduction, be moved back into again enforcement separation in solution bottle, enter sample size capillaceous and can only lean on rough estimation, therefore two kinds of input modes all lack accuracy; 2., while " dipping " sample introduction, in moving process, great changes will take place for the meeting affected by environment of the sample drop of kapillary port size, makes this sample introduction degree of accuracy (repeatability) very poor; 3. must cut off the high-voltage electric field that puts on kapillary two ends during sample introduction, make the electric field having established be interrupted again and re-establish every now and then, cause the precision of analysis result undesirable; 3. " dip " formula sample introduction and easily cause the mutual pollution between sample and damping fluid and different sample; 4. electromigration injection mode can produce electric Discrimination Phenomenon, thereby further reduces accuracy and the reliability of analyzing.
In a word, the sample size of capillary electrophoresis apparatus is very little, is all to receive liter (nl) level, traditional input mode that existing capillary electrophoresis apparatus adopts cannot complete the accurate quantitative analysis of small size like this, Capillary dips the mode of sample introduction, and its sample introduction reproducibility error is large, is difficult to quantitative measurment.
Utility model content
Technical problem to be solved in the utility model is to overcome the deficiencies in the prior art, and a kind of full-automatic high precision capillary electrophoresis apparatus is provided, and realizes automatic quantitative sample, improves accuracy, reappearance and the reliability of sample introduction; Eliminate the mutual pollution between electric Discrimination Phenomenon and sample; Improve sample introduction speed, shorten sample injection time, avoid to greatest extent outside air to enter in sample introduction stream; Realize automatic cleaning and the balance of different reagent to capillary separation column simultaneously.
The scheme that the utility model solves its technical matters is as follows:
A kind of full-automatic high precision capillary electrophoresis apparatus, include electrophoresis system, this electrophoresis system comprises that outlet inserts the electrophoretic separation kapillary of damping fluid bottle with liquid-out, is connected to column oven and detecting device on this electrophoretic separation kapillary in turn, one utmost point of one high-voltage power supply inserts in described damping fluid bottle with liquid-out, another utmost point is connected described electrophoretic separation kapillary and formed high-voltage electric field in this electrophoretic separation kapillary by electric spacer assembly, it is characterized in that:
Described capillary electrophoresis apparatus also includes the sample introduction stream being connected with described electrophoresis system and the automatic sampling stream being connected with this sample introduction stream;
Described sample introduction stream comprises shunting waste liquid bottle, four-way micro-sampling valve and the damping fluid syringe pump being connected respectively with three interfaces of a four-way connector;
Described four-way micro-sampling valve can accurate quantitative analysis, be used for to described electrophoretic separation kapillary quantitative sample injection, include S port, W port, P port and C port be totally four stationary pipes road ports and rotatable built-in quantitative ring, this C port is connected with described electrophoretic separation kapillary, this P port is connected with described four-way connector, this built-in quantitative ring is provided with the quantitative path that a bypass path and has fixed volume, rotation with described built-in quantitative ring, described bypass path and quantitatively path be communicated with described S port and W port and be communicated with P port and C port between switch,
Described automatic sampling stream comprises sampling probe, cleaning fluid bottle, reagent bottle and six end liquid distributor and the syringe pumps that coupled together by the two ends with the separator tube of quantitative function;
Described syringe pump is furnished with the three end distribution valve that can switch exhaust and cleaning function, and three valve interfaces of this three ends distribution valve connect respectively described cleaning fluid bottle, automatic sampler waste liquid bottle and separator tube;
Described six end liquid distributors have a fixed interface and six distribution interface that can be connected with this fixed interface wheel stream translation, this fixed interface connects described separator tube, and these six distribution interface connect respectively an interface of described sampling probe, four-way connector, the S port of four-way micro-sampling valve and three reagent bottles that are equipped with in order to electrophoretic separation different reagent capillaceous described in cleaning and balance;
Between described cleaning fluid bottle and automatic sampler waste liquid bottle, be connected with to clean the rinse bath of described sampling probe, described sampling probe can switch the sample disc of inserting this rinse bath or different samples being housed;
Described automatic sampler waste liquid bottle is connected with the W port of described four-way micro-sampling valve.
As further improvement, described electrophoresis system also comprises balance waste liquid bottle, and the outlet capillaceous of described electrophoretic separation can be switched this balance waste liquid bottle of connection or damping fluid bottle with liquid-out.
As further improvement, the volume of the quantitative path of described four-way micro-sampling valve is that 1nL is to any volume between 20nL.
As further improvement, between described damping fluid syringe pump and four-way connector, be connected with the capillary pressure sensor for detection of working pressure of pipeline, between three end distribution valve of described syringe pump and separator tube, be connected with the sampling stream pressure transducer that detects sampling stream working pressure.
As further improvement, described sample disc has constant temperature and refrigeration structure.
As further improvement, described cleaning fluid is bottled cleaning fluid, and this cleaning fluid is ethanol or deionized water.
As further improvement, the function of the described all members of capillary electrophoresis apparatus is realized automatically and being controlled by computer program.
As further improvement, between described four-way connector and shunting waste liquid bottle, be connected with the isocon in order to electrophoretic separation kapillary internal pressure described in balance.
As further improvement, described detecting device is detecting device on UV-detector or other capillary column.
Compare with traditional capillary electrophoresis apparatus, automatic sampling stream of the present utility model is combined use by a syringe pump with three end distribution valve and six end liquid distributors, by sample first in the separator tube between the two of the quick suction of pipeline by large latus rectum, then switch the path of six end liquid distributors, sample is squeezed in four-way micro-sampling valve by malleation, thereby the shortcomings such as sample introduction speed that Ngatively pressurized sampling causes is slow have been overcome, greatly shorten sample injection time, avoid to greatest extent outside air to enter in sample introduction stream simultaneously, increased substantially the reliability of sample introduction, realized automatic cleaning and the balance of different reagent to capillary separation column, eliminated the mutual pollution between electric Discrimination Phenomenon and sample.In addition, the utility model has adopted the four-way micro-sampling valve of upgrading of receiving of a built-in quantitative ring, its quantitative volume is that 1nL is to the arbitrary definite volume between 20nL, by its switching, completed accurate quantitative sample injection, input mode of the present utility model has overcome that traditional Capillary dips or gaseous tension input mode can not complete the shortcoming of the accurate sample introduction of small size like this, realized automatic quantitative sample, improved the reappearance of sample introduction, sample introduction repeatability relative standard deviation has reached below 3%.
Full-automatic high precision capillary electrophoresis apparatus described in the utility model has advantages of that sample introduction speed is fast, quantitative precision good, degree of accuracy is high, favorable reproducibility and be easy to industrialization, realize the robotization of sample feeding, kapillary cleaning and balance, can be widely used in the capillary electrophoresis analysis of different material.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is one of course of work schematic diagram of the present utility model.
Fig. 3 is two of course of work schematic diagram of the present utility model.
Fig. 4 is three of course of work schematic diagram of the present utility model.
Fig. 5 is four of course of work schematic diagram of the present utility model.
Fig. 6 is five of course of work schematic diagram of the present utility model.
Fig. 7 is six of course of work schematic diagram of the present utility model.
Fig. 8 is seven of course of work schematic diagram of the present utility model.
In figure,
1 damping fluid syringe pump, 2 capillary pressure sensors, 3 four-way connectors, 4 shunting waste liquid bottles,
5 four-way micro-sampling valves, 51 quantitative paths, 52 bypass paths, 6 electric spacer assemblys,
7 electrophoretic separation kapillaries, 8 column ovens, 9 high-voltage power supplies, 10 detecting devices,
11 damping fluid bottle with liquid-out, 12 balance waste liquid bottles, 13 6 end liquid distributors, 14 separator tubes,
15 sampling probes, 16 sample disc, 17 rinse baths, 18 sampling stream pressure transducers,
19 syringe pumps, 20 reagent bottles, 21 cleaning fluid bottles, 22 automatic sampler waste liquid bottles,
23 isocons.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in detail; but following embodiment is not used for limiting scope of the present utility model; all equivalences of making according to the content of this instructions change and revise, and all should belong to the claimed scope of the utility model patented claim.
Full-automatic high precision capillary electrophoresis apparatus described in the utility model is for the capillary electrophoresis analysis of different material.Refer to Fig. 1, diagram full-automatic high precision capillary electrophoresis apparatus includes electrophoresis system, sample introduction stream and automatic sampling stream, and this sample introduction stream is connected with described electrophoresis system, and this automatic sampling stream is connected with described sample introduction stream.
Described electrophoresis system comprises electrophoretic separation kapillary 7, column oven 8, detecting device 10, high-voltage power supply 9, electric spacer assembly 6, damping fluid bottle with liquid-out 11 and balance waste liquid bottle 12.Described electrophoretic separation kapillary 7 is places of material capillary electrophoresis separation, is also the core original paper that native system carries out compartment analysis, and described damping fluid bottle with liquid-out 11 is inserted in its outlet; 8 pairs of electrophoretic separation kapillaries of described column oven 7 carry out temperature control, are mainly the Joule heats producing in order to shed when some electrophoretic separation, and the low temperature crystallization of heating kapillary inner material while preventing capillary electrophoresis separation experiment; Described detecting device 10, for the detection to signal after separation, can be detecting device on UV-detector or other capillary column, and this column oven 8 and detecting device 10 are connected in turn on described electrophoretic separation kapillary 7; The outlet of electrophoretic separation kapillary 7 when described damping fluid bottle with liquid-out 11 is analyzed for electrophoretic separation; One utmost point of described high-voltage power supply 9 inserts in described damping fluid bottle with liquid-out 11, and another utmost point connects described electrophoretic separation kapillary 7 by electric spacer assembly 6, to form high-voltage electric field in this 7 electrophoretic separation kapillary; The outlet of described electrophoretic separation kapillary 7 can be switched the described balance waste liquid bottle 12 of connection or damping fluid bottle with liquid-out 11, when cleaning or balance electrophoretic separation kapillary 7, the outlet of electrophoretic separation kapillary 7 need to be switched in balance waste liquid bottle 12, otherwise can affect the pH value of damping fluid in damping fluid bottle with liquid-out 11, thereby affect measurement result.
Described sample introduction stream comprises damping fluid syringe pump 1, capillary pressure sensor 2, four-way micro-sampling valve 5, four-way connector 3 and shunting waste liquid bottle 4.Described damping fluid syringe pump 1 supplements damping fluid and certain pressure for providing to described electrophoretic separation kapillary 7; Described capillary pressure sensor 2 is for detection of the working pressure of described electrophoretic separation kapillary 7 and distribution pipeline; Described shunting waste liquid bottle 4 is for holding by the damping fluid after described four-way connector 3 shuntings.
Described four-way micro-sampling valve 5 has accurate quantitative analysis function, include S port, W port, P port and C port totally four stationary pipes road ports and rotatable built-in quantitative ring, this built-in quantitative ring is provided with a bypass path 52 and a certain amount of path 51, this quantitative path 51 has 1nL to the volume of the arbitrary fixed amount between 20nL, as 4nL, 10nL or 20nL, with realize receive upgrading quantitative sampling; Rotation with described built-in quantitative ring, described bypass path 52 and quantitatively path 51 be communicated with described S port and W port and be communicated with P port and C port between switch, specifically, when position at topping up, described quantitative path 51 is communicated with described S port and W port, simultaneously described bypass path 52 is communicated with described P port and C port, when the position of built-in quantitative ring Rotate 180 ° in fluid injection, described bypass path 52 is communicated with described S port and W port, and described quantitative path 51 is communicated with described P port and C port; The C port of described four-way micro-sampling valve 5 is connected with described electrophoretic separation kapillary 7, for carrying out quantitative sample injection to described electrophoretic separation kapillary 7.
Three interfaces of described four-way connector 3 connect respectively the P port of described damping fluid syringe pump 1, shunting waste liquid bottle 4 and four-way micro-sampling valve 5; Described capillary pressure sensor 2 is connected between described damping fluid syringe pump 1 and four-way connector 3; between the P port of described four-way connector 3 and four-way micro-sampling valve 5, be connected with a filtrator; in order to the large granular impurity in filtered sample neutralization reagent; protection four-way micro-sampling valve 5 and electrophoretic separation kapillary 7; between described four-way connector 3 and shunting waste liquid bottle 4, be connected with isocon 23, this isocon 23 is by changing bore and length with the internal pressure of electrophoretic separation kapillary 7 described in balance.
Described automatic sampling stream comprises six end liquid distributors 13, sampling probe 15, sample disc 16, rinse bath 17, separator tube 14, reagent bottle 20, sampling stream pressure transducer 18, syringe pump 19, cleaning fluid bottle 21 and automatic sampler waste liquid bottle 22.
Described six end liquid distributors 13 have a fixed interface and six distribution interface that can be connected with this fixed interface wheel stream translation; This fixed interface connects described separator tube 14, and these six distribution interface connect respectively an interface of described sampling probe 15, four-way connector 3, the S port of four-way micro-sampling valve 5 and three reagent bottles 20 that are equipped with in order to the different reagent of electrophoretic separation kapillary 7 described in cleaning and balance.
Described syringe pump 19 is the source of whole system stream power, it is furnished with the three end distribution valve that can switch completion system exhaust and cleaning function, and three valve interfaces of this three ends distribution valve connect respectively described cleaning fluid bottle 21, automatic sampler waste liquid bottle 22 and separator tube 14.Described sampling stream pressure transducer 18 is for detection of the working pressure of sampling stream, and it is connected between the three end distribution valve and separator tube 14 of described syringe pump 19.
Described cleaning fluid bottle 21 is equipped with cleaning fluid, and this cleaning fluid is also the mobile phase of sample introduction stream, generally selects ethanol or deionized water.Waste liquid after described automatic sampler waste liquid bottle 22 cleans for containment, it is connected with the W port of described four-way micro-sampling valve 5.Described rinse bath 17 is in order to complete the cleaning of outer wall and the inwall of described sampling probe 15 simultaneously, and it is connected between described cleaning fluid bottle 21 and automatic sampler waste liquid bottle 22.
Described separator tube 14 has certain quantitative function, for deposit the place of liquid such as comprising sample, sample introduction mobile phase, reagent, cleaning fluid in system temporarily; The two ends of this separator tube 14 couple together described six end liquid distributors 13 and syringe pump 19, by the collaborative work of this combined member, and can completion system exhaust, the function such as cleaning (balance), automatic quantitative sample.
Described sample disc 16 is equipped with different samples, and it has constant temperature and refrigeration structure, can make sample remain on very low temperature, keeps the activity of biology enzyme, reduces the volatilization of sample; Described sampling probe 15 has puncture function, can be deep into through the rubber bottle cover of sample bottle the inner sampling of sample bottle, and this sampling probe 15 can switch the described rinse bath 17 of insertion or sample disc 16.
The function of all members of described capillary electrophoresis apparatus is all realized automatically and being controlled by computer program, thereby reaches fully automatic operation of the present utility model.
The principle of work of full-automatic high precision capillary electrophoresis apparatus described in the utility model is as follows:
The first step, referring to Fig. 2, the endpiece of described electrophoretic separation kapillary 7 switches in damping fluid bottle with liquid-out 11, and described four-way micro-sampling valve 5 is positioned at the position of topping up, and the quantitative path in described four-way micro-sampling valve 5 51 is communicated with described S port and W port; Described six end liquid distributor 13 conversion connection sampling probes 15, this sampling probe 15 inserts in sample disc 16, described syringe pump 19 sucks the sample (being generally not less than the volume of 1.5 times of sampling probes 15) of " displaced volume ", make it to be full of sampling probe 15, and then continue a certain amount of air of suction, sample is entered in described separator tube 14 completely; Thereby with the inwall of sample wash sampling probe 15, displace cleaning fluid and interfering component in pipeline, make it can not be injected in separated stream.
Second step, referring to Fig. 3, the S end of described six end liquid distributor 13 conversion connection four-way micro-sampling valves 5, the sample that syringe pump 19 is released " displaced volume " enters in the built-in quantitative ring of four-way micro-sampling valve 5, make sample be full of described quantitative path 51 and reach quantitative, the inwall of sample wash four-way micro-sampling valve 5, releases the described automatic sampler waste liquid bottle 22 of W end inflow of four-way micro-sampling valve 5 by the cleaning fluid in pipeline and interfering component, and can not be injected in separated stream simultaneously.
The 3rd step, referring to Fig. 4, described built-in quantitative ring Rotate 180 °, four-way micro-sampling valve 5 is switched to the position of fluid injection, and the quantitative path 51 that is full of sample in four-way micro-sampling valve 5 is communicated with described P port and C port, with described electrophoretic separation kapillary 7 UNICOMs; The pressure of described damping fluid syringe pump 1 makes the sample in quantitative path 51 enter electrophoretic separation kapillary 7, starts to carry out the quantitative electrophoresis analysis of sample; Meanwhile, described sampling probe 15 moves in rinse bath 17.
The 4th step, referring to Fig. 5, after the sample in quantitative path 51 is all released, described built-in quantitative ring Rotate 180 °, four-way micro-sampling valve 5 switches the position of getting back to topping up, and described quantitative path 51 is communicated with described S port and W port; When carrying out capillary electrophoresis analysis, described six end liquid distributors 13 keep being communicated with the S end of four-way micro-sampling valve 5, syringe pump 19 injects four-way micro-sampling valve 5 by the cleaning fluid in cleaning fluid bottle 21, so that its built-in quantitative ring is carried out to low discharge cleaning.
The 5th step, referring to Fig. 6, when carrying out capillary electrophoresis analysis, described six end liquid distributor 13 conversion connections insert the sampling probe 15 of described rinse bath 17, and described syringe pump 19 injects sampling probe 15 so that sampling probe 15 and sampling stream are cleaned by cleaning fluid.
The 6th step, referring to Fig. 7, after capillary electrophoresis analysis completes, enters reagent and cleans and 7 stages of balance electrophoretic separation kapillary; The needed reagent bottle 20 of described six end liquid distributor 13 conversion connection, sucks the reagent of cleaning and balance electrophoretic separation kapillary 7 use in separator tube 14 with syringe pump 19.
The 7th step, referring to Fig. 8, the endpiece of described electrophoretic separation kapillary 7 switches to balance waste liquid bottle 12, described six end liquid distributor 13 conversion connection four-way connectors 3, with described syringe pump 19, with specific speed, by the reagent in separator tube 14, the bypass path 52 by described four-way micro-sampling valve 5 pushes electrophoretic separation kapillary 7, electrophoretic separation kapillary 7 is cleaned and balance, and the waste liquid after cleaning enters described balance waste liquid bottle 12.
The 8th step, still see Fig. 8, described six end liquid distributors 13 keep communicating with four-way connector 3, with syringe pump 19, the cleaning fluid in described cleaning fluid bottle 21 are injected to separator tube 14, and then inject electrophoretic separation kapillary 7, thereby whole separator tube 14 and electrophoretic separation kapillary 7 are cleaned.
Complete after cleaning, described full-automatic high precision capillary electrophoresis apparatus reduction original state, can start from the first step electrophoretic analysis of a new round.
In the above-mentioned course of work, the function of all members of described capillary electrophoresis apparatus is all realized automatically and being controlled by computer program, thereby reaches fully automatic operation.
The protection domain that the utility model requires is not limited only to above-described embodiment, also should comprise other apparent conversion and replacement schemes.

Claims (9)

1. a full-automatic high precision capillary electrophoresis apparatus, include electrophoresis system, this electrophoresis system comprises that outlet inserts the electrophoretic separation kapillary of damping fluid bottle with liquid-out, is connected to column oven and detecting device on this electrophoretic separation kapillary in turn, one utmost point of one high-voltage power supply inserts in described damping fluid bottle with liquid-out, another utmost point is connected described electrophoretic separation kapillary and formed high-voltage electric field in this electrophoretic separation kapillary by electric spacer assembly, it is characterized in that:
Described capillary electrophoresis apparatus also includes the sample introduction stream being connected with described electrophoresis system and the automatic sampling stream being connected with this sample introduction stream;
Described sample introduction stream comprises shunting waste liquid bottle, four-way micro-sampling valve and the damping fluid syringe pump being connected respectively with three interfaces of a four-way connector;
Described four-way micro-sampling valve can accurate quantitative analysis, be used for to described electrophoretic separation kapillary quantitative sample injection, include S port, W port, P port and C port be totally four stationary pipes road ports and rotatable built-in quantitative ring, this C port is connected with described electrophoretic separation kapillary, this P port is connected with described four-way connector, this built-in quantitative ring is provided with the quantitative path that a bypass path and has fixed volume, rotation with described built-in quantitative ring, described bypass path and quantitatively path be communicated with described S port and W port and be communicated with P port and C port between switch,
Described automatic sampling stream comprises sampling probe, cleaning fluid bottle, reagent bottle and six end liquid distributor and the syringe pumps that coupled together by the two ends with the separator tube of quantitative function;
Described syringe pump is furnished with the three end distribution valve that can switch exhaust and cleaning function, and three valve interfaces of this three ends distribution valve connect respectively described cleaning fluid bottle, automatic sampler waste liquid bottle and separator tube;
Described six end liquid distributors have a fixed interface and six distribution interface that can be connected with this fixed interface wheel stream translation, this fixed interface connects described separator tube, and these six distribution interface connect respectively an interface of described sampling probe, four-way connector, the S port of four-way micro-sampling valve and three reagent bottles that are equipped with in order to electrophoretic separation different reagent capillaceous described in cleaning and balance;
Between described cleaning fluid bottle and automatic sampler waste liquid bottle, be connected with to clean the rinse bath of described sampling probe, described sampling probe can switch the sample disc of inserting this rinse bath or different samples being housed;
Described automatic sampler waste liquid bottle is connected with the W port of described four-way micro-sampling valve.
2. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, is characterized in that: described electrophoresis system also comprises balance waste liquid bottle, and the outlet capillaceous of described electrophoretic separation can be switched this balance waste liquid bottle of connection or damping fluid bottle with liquid-out.
3. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1 or 2, is characterized in that: the volume of the quantitative path of described four-way micro-sampling valve is that 1nL is to any volume between 20nL.
4. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, it is characterized in that: between described damping fluid syringe pump and four-way connector, be connected with the capillary pressure sensor for detection of working pressure of pipeline, between three end distribution valve of described syringe pump and separator tube, be connected with the sampling stream pressure transducer that detects sampling stream working pressure.
5. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, is characterized in that: described sample disc has constant temperature and refrigeration structure.
6. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, is characterized in that: described cleaning fluid is bottled cleaning fluid, and this cleaning fluid is ethanol or deionized water.
7. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, is characterized in that: the function of the described all members of capillary electrophoresis apparatus is realized automatically and being controlled by computer program.
8. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, is characterized in that: between described four-way connector and shunting waste liquid bottle, be connected with the isocon in order to electrophoretic separation kapillary internal pressure described in balance.
9. full-automatic high precision capillary electrophoresis apparatus as claimed in claim 1, is characterized in that: described detecting device is detecting device on UV-detector or other capillary column.
CN201420131764.XU 2014-03-21 2014-03-21 Full-automatic high-precision capillary tube electrophoresis apparatus Active CN203786079U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103868970A (en) * 2014-03-21 2014-06-18 上海通微分析技术有限公司 Full-automatic high-precision capillary electrophoresis apparatus
CN104237359A (en) * 2014-09-18 2014-12-24 上海交通大学 Exhaust device for anti-leakage electrophoresis titration electrode liquid flow and use method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103868970A (en) * 2014-03-21 2014-06-18 上海通微分析技术有限公司 Full-automatic high-precision capillary electrophoresis apparatus
WO2015139156A1 (en) * 2014-03-21 2015-09-24 阎超 Fully automated high-precision capillary electrophoresis instrument
CN103868970B (en) * 2014-03-21 2016-01-06 上海通微分析技术有限公司 Full-automatic high precision capillary electrophoresis apparatus
US9903835B2 (en) 2014-03-21 2018-02-27 Chao Yan Fully automated high-precision capillary electrophoresis instrument
CN104237359A (en) * 2014-09-18 2014-12-24 上海交通大学 Exhaust device for anti-leakage electrophoresis titration electrode liquid flow and use method thereof

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