CN102901835B - Method and device for collecting sample by full-automatic blood cell analyzer - Google Patents

Method and device for collecting sample by full-automatic blood cell analyzer Download PDF

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Publication number
CN102901835B
CN102901835B CN201210389633.7A CN201210389633A CN102901835B CN 102901835 B CN102901835 B CN 102901835B CN 201210389633 A CN201210389633 A CN 201210389633A CN 102901835 B CN102901835 B CN 102901835B
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China
Prior art keywords
sampling probe
dilution
wall washing
whole blood
blood sample
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Expired - Fee Related
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CN201210389633.7A
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Chinese (zh)
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CN102901835A (en
Inventor
王振宇
李传龙
荆全振
刘拯
陈忠武
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SHANDONG MEIYILIN ELECTRONIC INSTRUMENT CO Ltd
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SHANDONG MEIYILIN ELECTRONIC INSTRUMENT CO Ltd
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Publication of CN102901835A publication Critical patent/CN102901835A/en
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Abstract

The invention discloses a method and a device for collecting a sample by a full-automatic blood cell analyzer, belongs to a blood detection device, and particularly relates to a method and a device for collecting the sample by the full-automatic blood cell analyzer used in a blood routine test. The device comprises a sampling needle, and an inner hole of the sampling needle is connected with a diluent injection device through a pipeline. The method is characterized by comprising the steps that a sampling needle outer wall cleaning element is sleeved outside the sampling needle; a waste fluid outlet and a diluent inlet for cleaning in up and down arrangement are arranged at the lateral wall of a housing of the sampling needle outer wall cleaning element; the waste liquid outlet is connected with a waste liquid collecting device, and the diluent inlet for cleaning is connected with a diluent supply device. The sampling needle is full of diluent; the diluent and a whole blood sample are isolated by an air column; the outer wall of the sampling needle is cleaned by the sampling needle outer wall cleaning element; the front end of the whole blood sample is removed before the whole blood sample is injected; and the inner wall of the sampling needle is cleaned after the whole blood sample is injected, so that the metering accuracy for sample collection is ensured.

Description

A kind of method and device that is applicable to the sample collection of Automatic Blood Cell Analyzer
Technical field
The invention belongs to blood testing equipment, method and the device of the sample collection of the Automatic Blood Cell Analyzer particularly using in clinical examination routine blood test project.
Background technology
The Automatic Blood Cell Analyzer using in clinical examination routine blood test project requires trace blood, three classification or five classification cellanalyzers approximately need to be used 10-60 microlitre whole blood sample to measure, so the sample collection of trace is easy to occur following problem: be 1. present in the air in sampling probe pipeline, in syringe pump sample drawn, existence due to pressure reduction, the cumulative volume of air can change, the quantity that affects sample collection is inaccurate, when 2. extracting at every turn and annotating sample, the amount of sampling probe inwall or the residual sample of outer wall is inconsistent, can cause the inaccurate problem of application of sample, if 3. sampling probe inwall is full of dilution, after dilution liquid level contacts with sample liquid level, can mutually permeate, change the concentration of whole blood sample, when whole blood sample need to be filled into respectively to different measuring mechanisms, whole blood sample can lengthen in the sampling probe internal stops time, and along with each filling whole blood sample, it is also just more serious mutually to there is infiltration in cleaning fluid and whole blood sample, caused the concentration of whole blood sample of each filling inconsistent.
Summary of the invention
In order to overcome in existing three classification or five classification cellanalyzer sample collections owing to there being air in sampling probe, the residual sample of sampling probe inside and outside wall causes metering inaccurate, though solved measurement problem while using dilution in sampling probe but dilution can occur can interpenetrate with sample intersection the deficiency that causes change whole blood sample concentration, the object of this invention is to provide a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, another object of the present invention is to provide a kind of method that is applicable to the sample collection of Automatic Blood Cell Analyzer of using this device.
Technical scheme of the present invention is, a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe, sampling probe endoporus is connected with dilution filing provision by pipeline, it is characterized in that: sampling probe overcoat has sampling probe outer wall washing part, the waste liquid outlet being arranged above and below is housed in sampling probe outer wall washing part housing sidewall and cleans and use dilution import, waste liquid outlet is connected with waste collecting device, cleans with dilution import and is connected with dilution feedway.
Described waste collecting device comprises waste drains pump and the waste liquid bottle being connected with waste liquid outlet.
Described sampling probe is equipped with sampling probe O-ring seal outward.
Between the syringe pump push rod of described syringe pump and the injection pump housing, sealing syringe pump O-ring seal is housed.
Described dilution filing provision comprises syringe pump, and the end of syringe pump has dilution inlet pipe road to be connected with diluting pump, and inner wall washing solenoid valve is housed between dilution inlet pipe road and diluting pump, and diluting pump is connected with dilution bottle.
The cleaning of described dilution feedway is connected to and cleans with in dilution import with dilution pipeline one end, and the other end is connected with dilution bottle with diluting pump through outer wall washing solenoid valve.
Use the sample collection method of the device of aforesaid a kind of sample collection that is applicable to Automatic Blood Cell Analyzer, it is characterized in that: while drawing whole blood sample, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing part, syringe pump push rod is descending, make the upper end of syringe pump push rod lower than dilution inlet pipe road, waste drains pump operation produces negative pressure, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing part, inner wall washing solenoid valve powers on and opens, diluting pump operation, dilution flows out from sampling probe is inner, waste liquid outlet at sampling probe outer wall washing part is pumped, inner wall washing is complete, diluting pump is out of service, the power-off of inner wall washing solenoid valve is closed, syringe pump push rod is up to topmost, descending a bit of again, draw micro-air, to form air barrier, it is inner that sampling probe goes downwards to whole blood sample test tube, the whole blood sample that the descending absorption of syringe pump push rod is quantitative, sampling probe is up to the centre position of sampling probe outer wall washing part, in the up process of sampling probe, outer wall washing solenoid valve powers on and opens, diluting pump operation, clean the whole blood sample adhering on sampling probe outer wall, after cleaning, diluting pump stops, the power-off of outer wall washing solenoid valve is closed, after carrying on sampling probe and stopping, syringe pump push rod is operation upwards, the whole blood sample front end of sampling probe inside is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet of sampling probe outer wall washing part, waste drains pump is closed, stop producing negative pressure, sampling probe translation, whole blood sample layer is filled into respectively in each different measuring mechanism, after the filling of whole blood sample layer, sampling probe is up to the centre position of sampling probe outer wall washing part, waste drains pump operation produces negative pressure, syringe pump push rod is descending, make the upper end of syringe pump push rod lower than dilution inlet pipe road, on inner wall washing solenoid valve, conduct, diluting pump operation, the inner new cleaning fluid injecting of sampling probe is pushed away whole blood sample end, and the waste liquid outlet of sampled pin outer wall washing part is taken out, complete a sample collection filling and sampling probe cleaning process.
The present invention adopts sampling probe inner space to be full of dilution, before sample drawn, syringe pump first extracts micro-air, then extracts whole blood sample, the air column forming is used for isolating dilution and whole blood sample, prevents dilution and whole blood sample permeates mutually.
In order to solve the residual inconsistent problem of whole blood sample, the whole blood sample that the present invention uses sampling probe outer wall washing part and dilution to adhere to sampling probe outer wall cleans, because the front end of the whole blood sample of sampling probe inside and end sample exist and contact on a small quantity with dilution, so before filling whole blood sample, first the front-end samples of the whole blood sample of sampling probe inside is taken out in sampling probe outer wall washing part, after whole blood sample filling, the end sample of the whole blood sample of sampling probe inside is taken out in the process of dilution cleaning sampling probe inwall.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention,
Fig. 2 is inner each liquid layer distribution plan of sampling probe.
In figure, 1. sampling probe, 2. sampling probe O-ring seal, 3. sampling probe outer wall washing part, 4. waste drains pump, 5. waste liquid bottle, 6. syringe pump push rod, 7. syringe pump O-ring seal, 8. syringe pump dilution liquid layer, 9. dilution inlet pipe road, 10. diluting pump, 11. dilution bottles, 12. inner wall washing solenoid valves, 13. outer wall washing solenoid valves, 14. sampling probe dilution liquid layers, 15. air barrier, 16. whole blood sample layers, 17. whole blood sample front ends, 18. whole blood sample ends, 19. waste liquid outlets, 20. cleaning dilution imports.
Embodiment
The specific embodiment of the present invention is, as shown in the figure:
Embodiment 1, a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe 1, sampling probe endoporus is connected with dilution filing provision by pipeline, it is characterized in that: sampling probe overcoat has sampling probe outer wall washing part 3, the waste liquid outlet 19 being arranged above and below is housed in sampling probe outer wall washing part housing sidewall and cleans with dilution import 20, waste liquid outlet is connected with waste collecting device, cleans with dilution import and is connected with dilution feedway.
Embodiment 2, a kind of device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe 1, sampling probe endoporus is connected with dilution filing provision by pipeline, it is characterized in that: sampling probe overcoat has sampling probe outer wall washing part 3, the waste liquid outlet 19 being arranged above and below is housed in sampling probe outer wall washing part housing sidewall and cleans with dilution import 20, waste liquid outlet is connected with waste collecting device, cleans with dilution import and is connected with dilution feedway.Described waste collecting device comprises waste drains pump 4 and the waste liquid bottle 5 being connected with waste liquid outlet.Described sampling probe is equipped with sampling probe O-ring seal 2 outward.Between the syringe pump push rod 6 of described syringe pump and the injection pump housing, syringe pump O-ring seal 7 for sealing is housed.Described dilution filing provision comprises syringe pump, and the end of syringe pump has dilution inlet pipe road 9 to be connected with diluting pump 10, and inner wall washing solenoid valve 12 is housed between dilution inlet pipe road and diluting pump, and diluting pump is connected with dilution bottle 11.The cleaning of described dilution feedway is connected to and cleans with in dilution import with dilution pipeline one end, and the other end is connected with dilution bottle with diluting pump through outer wall washing solenoid valve.
Embodiment 3, use the sample collection method of the device of aforesaid a kind of sample collection that is applicable to Automatic Blood Cell Analyzer, it is characterized in that: while drawing whole blood sample, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing part, syringe pump push rod is descending, make the upper end of syringe pump push rod lower than dilution inlet pipe road, waste drains pump operation produces negative pressure, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing part, inner wall washing solenoid valve powers on and opens, diluting pump operation, dilution flows out from sampling probe is inner, waste liquid outlet at sampling probe outer wall washing part is pumped, inner wall washing is complete, diluting pump is out of service, the power-off of inner wall washing solenoid valve is closed, syringe pump push rod is up to topmost, descending a bit of again, draw micro-air, to form air barrier, it is inner that sampling probe goes downwards to whole blood sample test tube, the whole blood sample that the descending absorption of syringe pump push rod is quantitative, sampling probe is up to the centre position of sampling probe outer wall washing part, in the up process of sampling probe, outer wall washing solenoid valve powers on and opens, diluting pump operation, clean the whole blood sample adhering on sampling probe outer wall, after cleaning, diluting pump stops, the power-off of outer wall washing solenoid valve is closed, after carrying on sampling probe and stopping, syringe pump push rod is operation upwards, the whole blood sample front end of sampling probe inside is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet of sampling probe outer wall washing part, waste drains pump is closed, stop producing negative pressure, sampling probe translation, whole blood sample layer is filled into respectively in each different measuring mechanism, after the filling of whole blood sample layer, sampling probe is up to the centre position of sampling probe outer wall washing part, waste drains pump operation produces negative pressure, syringe pump push rod is descending, make the upper end of syringe pump push rod lower than dilution inlet pipe road, on inner wall washing solenoid valve, conduct, diluting pump operation, the inner new cleaning fluid injecting of sampling probe is pushed away whole blood sample end, and the waste liquid outlet of sampled pin outer wall washing part is taken out, complete a sample collection filling and sampling probe cleaning process.
Specific operation process is as shown in Figure 1: inner wall washing solenoid valve 12 and outer wall washing solenoid valve 13 are normally close valve, on conduct, the end of syringe pump has dilution inlet pipe road 9 to be connected with diluting pump 10, between syringe pump push rod 6 and the injection pump housing, use syringe pump O-ring seal 7 to connect sealing, when syringe pump push rod 6 descending, the upper end that makes syringe pump push rod 6 is during lower than dilution inlet pipe road 9, on inner wall washing solenoid valve 12, conduct, diluting pump 10 operations, dilution in dilution bottle 11 can be filled in sampling probe 1 by syringe pump inner chamber and associated pipe, between sampling probe 1 and sampling probe outer wall washing part 3, use sampling probe O-ring seal 2 to connect sealing, the lower end of sampling probe 1 is retracted to the centre position of sampling probe outer wall washing part 3, waste drains pump 4 operations produce negative pressure, the inner cleaning fluid flowing out of sampling probe 1 can be extracted in waste liquid bottle 5 by waste liquid outlet 19.
When needs are drawn whole blood sample, the lower end of sampling probe 1 is retracted to the centre position of sampling probe outer wall washing part 3, syringe pump push rod 6 is descending, make the upper end of syringe pump push rod 6 lower than dilution inlet pipe road 9, waste drains pump 4 operations produce negative pressure, inner wall washing solenoid valve 12 powers on and opens, diluting pump 10 operations, dilution flows out from sampling probe is inner, waste liquid outlet 19 at sampling probe outer wall washing part 3 is pumped, inner wall washing is complete, diluting pump 10 is out of service, 12 power-off of inner wall washing solenoid valve are closed, syringe pump push rod 6 is up to topmost, descending a bit of again, draw micro-air, to form air barrier 15 as shown in Figure 2, due to sampling probe 1 inside instinct dilution almost, only there is a bit of air barrier 15, so when drawing whole blood sample, the variation of this fraction of volume of air affects very little on gathering the volume of whole blood sample, it is inner that sampling probe 1 goes downwards to whole blood sample test tube, the whole blood sample that the descending absorption of syringe pump push rod 6 is quantitative, sampling probe 1 is up to the centre position of sampling probe outer wall washing part 3, in the up process of sampling probe 1, outer wall washing solenoid valve 13 powers on and opens, diluting pump 10 operations, clean the whole blood sample adhering on sampling probe 1 outer wall, after cleaning, diluting pump 10 stops, 13 power-off of outer wall washing solenoid valve are closed.
As shown in Figure 2, between sampling probe dilution liquid layer 14 and whole blood sample end 18, there is air barrier 15, can well dilution and whole blood sample be separated, avoid interpenetrating, whole blood sample front end 17 is carried to the centre position of outer wall washing part 3 and is cleaned in outer wall process simultaneously and contacts with cleaning fluid on sampling probe 1, cause interpenetrating with cleaning fluid, so after carrying on sampling probe 1 and stopping, syringe pump push rod 6 upwards moves a bit of, the whole blood sample front end of sampling probe 1 inside 17 is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet 19 of sampling probe outer wall washing part 3, waste drains pump is closed, stop producing negative pressure, sampling probe 1 translation, whole blood sample layer 16 is filled into respectively in each different measuring mechanism, after 16 filling of whole blood sample layer, whole blood sample end 18 is also left in sampling probe 1 inside, sampling probe 1 is up to the centre position of sampling probe outer wall washing part 3, waste drains pump operation produces negative pressure, syringe pump push rod 6 is descending, make the upper end of syringe pump push rod 6 lower than dilution inlet pipe road 9, on inner wall washing solenoid valve 12, conduct, diluting pump 10 operations, the inner new cleaning fluid injecting of sampling probe 1 is pushed away whole blood sample end 18, and the waste liquid outlet 19 of sampled pin outer wall washing part 3 is taken out.

Claims (2)

1. a device that is applicable to the sample collection of Automatic Blood Cell Analyzer, comprise sampling probe, sampling probe endoporus is connected with dilution filing provision by pipeline, it is characterized in that: sampling probe overcoat has sampling probe outer wall washing part, the waste liquid outlet being arranged above and below is housed in sampling probe outer wall washing part housing sidewall and cleans and use dilution import, waste liquid outlet is connected with waste collecting device, cleans with dilution import and is connected with dilution feedway;
Described waste collecting device comprises waste drains pump and the waste liquid bottle being connected with waste liquid outlet;
Described sampling probe is equipped with sampling probe O-ring seal outward;
Described dilution filing provision comprises syringe pump, and the end of syringe pump has dilution inlet pipe road to be connected with diluting pump, and inner wall washing solenoid valve is housed between dilution inlet pipe road and diluting pump, and diluting pump is connected with dilution bottle;
Between the syringe pump push rod of described syringe pump and the injection pump housing, sealing syringe pump O-ring seal is housed;
The cleaning of described dilution feedway is connected to and cleans with in dilution import through outer wall washing solenoid valve with dilution pipeline one end, and the other end is connected on dilution inlet pipe road through inner wall washing solenoid valve.
2. right to use requires a kind of described in 1 to be applicable to the sample collection method of device of the sample collection of Automatic Blood Cell Analyzer, it is characterized in that: while drawing whole blood sample, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing part, syringe pump push rod is descending, make the upper end of syringe pump push rod lower than dilution inlet pipe road, waste drains pump operation produces negative pressure, the lower end of sampling probe is retracted to the centre position of sampling probe outer wall washing part, inner wall washing solenoid valve powers on and opens, diluting pump operation, dilution flows out from sampling probe is inner, waste liquid outlet at sampling probe outer wall washing part is pumped, inner wall washing is complete, diluting pump is out of service, the power-off of inner wall washing solenoid valve is closed, syringe pump push rod is up to topmost, descending a bit of again, draw micro-air, to form air barrier, it is inner that sampling probe goes downwards to whole blood sample test tube, the whole blood sample that the descending absorption of syringe pump push rod is quantitative, sampling probe is up to the centre position of sampling probe outer wall washing part, in the up process of sampling probe, outer wall washing solenoid valve powers on and opens, diluting pump operation, clean the whole blood sample adhering on sampling probe outer wall, after cleaning, diluting pump stops, the power-off of outer wall washing solenoid valve is closed, after carrying on sampling probe and stopping, syringe pump push rod is operation upwards, the whole blood sample front end of sampling probe inside is discharged, the whole blood sample of discharging is taken out by the waste liquid outlet of sampling probe outer wall washing part, waste drains pump is closed, stop producing negative pressure, sampling probe translation, whole blood sample layer is filled into respectively in each different measuring mechanism, after the filling of whole blood sample layer, sampling probe is up to the centre position of sampling probe outer wall washing part, waste drains pump operation produces negative pressure, syringe pump push rod is descending, make the upper end of syringe pump push rod lower than dilution inlet pipe road, on inner wall washing solenoid valve, conduct, diluting pump operation, the inner new cleaning fluid injecting of sampling probe is pushed away whole blood sample end, and the waste liquid outlet of sampled pin outer wall washing part is taken out, complete a sample collection filling and sampling probe cleaning process.
CN201210389633.7A 2012-10-15 2012-10-15 Method and device for collecting sample by full-automatic blood cell analyzer Expired - Fee Related CN102901835B (en)

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