CN107687963B - Method and device for confirming sample sampling reliability and blood cell analyzer - Google Patents
Method and device for confirming sample sampling reliability and blood cell analyzer Download PDFInfo
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- CN107687963B CN107687963B CN201610634882.6A CN201610634882A CN107687963B CN 107687963 B CN107687963 B CN 107687963B CN 201610634882 A CN201610634882 A CN 201610634882A CN 107687963 B CN107687963 B CN 107687963B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
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Abstract
The application discloses a method and a device for confirming sample sampling reliability and a blood cell analyzer, wherein the method comprises the steps of providing a sampling needle, the rear end of the sampling needle is connected with one end of a light transmission pipeline, the other end of the light transmission pipeline is connected with a power source, and a photoelectric sensing assembly is arranged on the light transmission pipeline; under the action of the power source, the sampling needle sucks a sample into the sampling needle from the front end of the sampling needle, and the sample sucking operation is finished; under the action of the power source, the sample in the sampling needle is pulled back, so that the sample is at least pulled to a photoelectric sensing assembly arranged on the light transmission pipeline; the photoelectric sensing assembly feeds back a detection signal; according to the signal of photoelectric sensing subassembly feedback, judge and inhale sample operation whether normal, consequently, this application provides a scheme whether normal of sample operation is inhaled in judgement simple operation convenience, with low costs.
Description
Technical Field
The application relates to a method and a device for confirming sample sampling reliability and a blood cell analyzer.
Background
A blood cell analyzer is an apparatus for analyzing a blood sample taken from a human body. To ensure the correct analysis result, it is first monitored whether the sample sucking and separating device for sucking and separating samples in the blood cell analyzer is normally sucking blood sample from a test tube container containing human blood sample (blood sample). The photoelectric technology with lower use cost is used for monitoring whether the sample sucking and separating device sucks blood samples normally, and two main schemes are provided at present.
Firstly, introduce sample and divide the appearance valve both sides to be provided with opto-coupler device in the sample respectively. The sample sucking and separating device quantifies a sample by using the sampling and separating valve, divides the sample into a plurality of equal parts, and monitors whether the sample is normally sucked or not by detecting whether the sample is detected by the optical coupling devices arranged on two sides of the sample separating valve or not in the process. Such protocols are typically only available on high-end hematology analyzers because of their high cost. In this case, since there is a flow path from the sampling needle to the sampling valve, a large amount of sample (consumption) is not used for analysis.
The second is to make the sampling needle into a shape of alternately being a light-transmitting part and a light-shielding part at regular intervals along the longitudinal direction, and then to confirm the liquid amount by receiving the light transmitted through the sampling needle by the light-emitting device through the light-receiving device. The sampling needle is made of transparent material such as quartz glass and hard transparent glass, and the surface thereof is sprayed with aluminum foil or the like at a predetermined interval in the longitudinal direction. The disadvantage of this solution is that the quartz glass and hard transparent glass lamp material are difficult to be processed precisely, and it is difficult to meet the requirement of the sampling needle processing precision.
For the metal sampling needle which can meet the requirement of processing precision, because the metal material is opaque, the sample-sucking reliability is difficult to be directly monitored by using a cheap photoelectric technology.
Disclosure of Invention
In order to solve the above problems, the present application provides a method and an apparatus for confirming sample sampling reliability, and a blood cell analyzer.
According to a first aspect of the present application, there is provided a method of confirming sample sampling reliability, comprising:
providing a sampling needle, wherein the rear end of the sampling needle is connected with one end of a light transmission pipeline, the other end of the light transmission pipeline is connected with a power source, and a photoelectric sensing assembly is arranged on the light transmission pipeline;
under the action of the power source, the sampling needle sucks a sample into the sampling needle from the front end of the sampling needle, and the sample sucking operation is finished;
under the action of the power source, the sample in the sampling needle is pulled back, so that the sample is at least pulled to a photoelectric sensing assembly arranged on the light transmission pipeline;
the photoelectric sensing assembly feeds back a detection signal;
and judging whether the operation of sucking the sample is normal or not according to the signal fed back by the photoelectric sensing assembly.
Preferably, the judging whether the operation of sucking the sample is normal or not according to the signal fed back by the photoelectric sensing assembly comprises: and when the curve of the fed back signal does not include the signal curve corresponding to the sample or the signal curve corresponding to the sample is abnormal, judging that the operation of sucking the sample is abnormal.
Preferably, before the sampling needle performs the sample sucking operation, an isolation gas column is also established in the sampling needle;
according to the signal of photoelectric sensing subassembly feedback, judge whether inhale sample operation normal, include: and when the curve of the fed back signal does not comprise a signal curve corresponding to the sample, or does not comprise a signal curve corresponding to the isolated gas column, or has an abnormality, judging that the operation of sucking the sample is abnormal.
Preferably, the light transmission pipeline is a transparent pipeline, and/or the photoelectric detection component comprises a light emitter and a light receiver, the light emitter and the light receiver form a light path, and at least one part of the light transmission pipeline passes through the light path.
Preferably, the method further comprises that under the action of the power source, part of the sample in the sampling needle is pushed forwards and discharged from the front-end needle tip so as to perform a sample dividing operation;
the back-pulling of the sample in the sampling needle is performed after the sample sucking operation and before the sample dividing operation, or after the sample dividing operation, or during the sample dividing operation.
According to a second aspect of the present application, there is provided a device for confirming sample sampling reliability, comprising a sampling needle and a power source, further comprising:
one end of the light transmission pipeline is connected with the rear end of the sampling needle, and the other end of the light transmission pipeline is connected with the power source;
the photoelectric sensing assembly is arranged on the light transmitting pipeline and used for feeding back a corresponding detection signal when a sample in the sampling needle is pulled backwards to at least the photoelectric sensing assembly on the light transmitting pipeline under the action of the power source after the sampling needle performs a sample sucking operation, wherein the sample is pulled backwards to at least the photoelectric sensing assembly and is positioned after the sampling needle sucks the sample and before the sample is divided, or is positioned after the sampling needle divides the sample, or is positioned in the sample dividing operation process of the sampling needle;
and the judging unit is used for judging whether the sample sucking operation is normal or not according to the signal fed back by the photoelectric sensing assembly.
Preferably, the judging unit judges that the operation of sucking the sample is abnormal when a signal curve corresponding to the sample is not included in a curve of the signal fed back by the photoelectric sensing assembly or the signal curve corresponding to the sample is abnormal.
Preferably, the judging unit judges that the operation of sucking the sample is abnormal when the curve of the signal fed back by the photoelectric sensing assembly does not include a signal curve corresponding to the sample, does not include a signal curve corresponding to the isolated gas column, or has abnormality; wherein before the sampling needle performs the sample suction operation, an isolation gas column is also established in the sampling needle.
Preferably, the light transmission pipeline is a transparent pipeline, and/or the photoelectric detection component comprises a light emitter and a light receiver, the light emitter and the light receiver form a light path, and at least one part of the light transmission pipeline passes through the light path.
Preferably, the photoelectric detection component is arranged at the rear end of the light transmission pipeline close to the sampling needle.
According to a third aspect of the present application, there is provided a blood cell analyzer comprising a sample sucking and separating device for sucking and separating a sample, a blood sample detecting device, an input device for inputting an instruction, and a display device, wherein the sample sucking and separating device comprises the device for confirming sample sampling reliability according to any one of claims 6 to 10, wherein the input device receives a test instruction, the sample sucking and separating device sucks a sample and supplies the sample to the blood detecting device, the blood detecting device detects the sample to obtain a sample detection result, and the display device displays the sample detection result.
Preferably, when the device for confirming the sampling reliability of the sample judges that the operation of the suction sample is abnormal:
the display device does not display the sample detection result or displays a mark with unreliable results near the sample detection result;
and/or the presence of a gas in the gas,
the device for confirming the sample sampling reliability sends an instruction to the sample sucking and separating device to suck the same sample again so as to provide the same sample for the blood detection device, or sends an instruction to the sample sucking and separating device to stop providing the sample for the blood detection device.
The beneficial effect of this application is:
according to the method, the device and the blood cell analyzer for confirming the sample sampling reliability, the light transmission pipeline is connected to the rear part of the sampling needle, and the photoelectric sensing assembly is arranged on the light transmission pipeline, so that when the sample sucking operation is judged to be normal, the sample in the sampling needle is only required to be pulled back to the photoelectric sensing assembly on the light transmission pipeline at least, and the sample sucking operation can be judged to be normal according to the signal fed back by the photoelectric sensing assembly, therefore, the scheme for judging whether the sample sucking operation is normal or not, which is simple and convenient to operate and low in cost, is provided; in addition, since the application draws the sample to the light-transmitting pipeline for detection, the application can also be applied to a light-tight sampling needle such as metal.
Drawings
FIG. 1 is a flow chart illustrating a method for confirming sample sampling reliability according to an embodiment of the present disclosure;
FIGS. 2(a), (b), and (c) are schematic diagrams of normal, abnormal, and abnormal signal curves of a sample sucking operation in a method for confirming sample sampling reliability according to an embodiment of the present application, respectively, where the ordinate is signal amplitude and the abscissa is time;
FIG. 3 is a flow chart illustrating a method for confirming sample sampling reliability according to another embodiment of the present application;
FIGS. 4(a), (b), (c), (d) are schematic diagrams of normal, abnormal and abnormal signal curves of the operation of the sample according to another embodiment of the present invention, wherein the ordinate is signal amplitude and the abscissa is time;
FIG. 5 is a schematic structural view of a blood cell analyzer according to an embodiment of the present application;
fig. 6 is a schematic structural diagram of an apparatus for confirming sample sampling reliability according to an embodiment of the present application.
Detailed Description
The invention of the application is characterized in that a light transmission pipeline is connected to the rear part of a sampling needle of a blood analyzer, a photoelectric sensing assembly is arranged on the light transmission pipeline, and a sample in the sampling needle is at least pulled back to the photoelectric sensing assembly of the light transmission pipeline, so that whether the sample sucking operation is normal or not is judged according to a signal fed back by the photoelectric sensing assembly. The most common opto-electronic sensing component is a pair of opto-coupler devices, which include an optical transmitter and an optical receiver, which are oppositely disposed on two sides of a light-transmitting pipeline, the optical transmitter and the optical receiver forming an optical path through which at least a portion of the light-transmitting pipeline passes. Other photoelectric sensing means capable of detecting changes in absorbance of the liquid in the conduit may also be suitable.
The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments.
Example one
The embodiment discloses a method for confirming the sample sampling reliability, which can be used for judging whether the sample sucking operation of a sampling needle is normal. In order to match the method of the embodiment, the rear end of the sampling needle in the embodiment is connected with one end of a light transmission pipeline, the other end of the light transmission pipeline is connected with a power source, and a photoelectric sensing assembly is arranged on the light transmission pipeline; wherein the power source is used for providing power for sucking a sample and discharging the sample for the sampling needle, and the most common power source is a syringe. Referring to fig. 1, the method for confirming the sample sampling reliability of the present embodiment specifically includes steps S101 to S111.
And step S101, under the action of a power source, the sampling needle performs sample suction operation from the front-end needle tip.
Step S103, under the action of a power source, the sample in the sampling needle is pushed forward and discharged from the front needle tip, so as to perform sample dividing operation.
And step S105, under the action of a power source, pulling back the sample in the sampling needle, so that the sample is pulled to at least the position of the photoelectric sensing assembly arranged on the light transmission pipeline. In order to make the signal fed back by the photoelectric sensing component clearer, in an embodiment, the light-transmitting pipeline may be a transparent pipeline. This step S105 may be performed after the sample sucking operation is performed in step S101 and before the sample dividing operation is performed in step S103; the sample division operation may be performed in step S103, for example, in step S103, the sucked sample is divided into several portions and respectively discharged into corresponding reaction containers, and after a certain sample is discharged into a corresponding reaction container, step S105 may be performed before the next sample is discharged into a corresponding reaction container; step S105 may also be performed after the full sample operation is performed at step S103. When the operation of pulling back the sample in the sampling needle in step S105 is performed after the sample splitting operation in step S103, this has the advantage of having minimal or no effect on the sample splitting operation. In one embodiment, the photoelectric sensing component is arranged at the rear end of the light transmission pipeline close to the sampling needle, so that the distance for pulling the sample is relatively reduced, and the influence on the sample is small. In one embodiment, the sample may be pulled back past the location of the photo-sensing assembly for better results.
And S107, feeding back a signal by the photoelectric sensing assembly. In one embodiment, the photoelectric sensing component may be in an operating state during the whole sample sucking operation and the sample dividing operation, or may start to operate when the sample in the sampling needle is pulled back in step S105. When the photoelectric sensing assembly works, signals can be continuously fed back.
And step S109, judging whether the sample sucking operation is normal or not according to the signal fed back by the photoelectric sensing assembly. In an embodiment, the step S109 includes determining that the operation of the sample sucking is abnormal when the curve of the signal fed back by the photoelectric sensing element does not include a signal curve corresponding to the sample or the signal curve corresponding to the sample is abnormal, and in an embodiment, after determining that the operation of the sample sucking is abnormal, performing step S111, alarming, and further shielding the measurement result of the sample. The step S109 may further include determining that the operation of sucking the sample is normal when a normal signal curve corresponding to the sample is included in the curve of the signal fed back by the photoelectric sensing assembly, and in an embodiment, after determining that the operation of sucking the sample is normal, performing step S113 to normally output the measurement result of the sample. In this embodiment, whether the sample sucking operation is normal is determined according to the signal fed back by the photoelectric sensing assembly, and through a great deal of research and practice, the inventor finds that, in the normal sample sucking operation, when the sample is pulled to the photoelectric sensing assembly passing through the light transmitting pipe, in the curve of the corresponding signal fed back by the photoelectric sensing assembly, the curve of the signal corresponding to the pulled-back sample is a curve with a length and a height within a certain range, as shown in fig. 2 (a); when a signal curve corresponding to a sample is not included, as shown in fig. 2(b), or an abnormality (e.g., abnormal jitter) exists in the signal curve corresponding to the sample, as shown in fig. 2(c), it is highly likely that an abnormal sample sucking operation occurs, where fig. 2(b) shows a case where the signal curve corresponding to the sample is not included in the corresponding signal curve, which indicates that the sample is likely not to be sucked by the sampling needle, and fig. 2(c) shows a case where the signal curve corresponding to the sample is abnormally jittered, which indicates that an abnormal condition exists in the sample sucking operation, for example, air is sucked while the sample is likely to be sucked by the sampling needle. Therefore, the case of FIG. 2(a) is judged that the suction sample operation is normal, and the cases of FIGS. 2(b) and (c) are judged that the suction sample operation is abnormal.
Example two
The embodiment also discloses a method for confirming the sampling reliability of a sample, which is different from the first embodiment in that when the sample sucking operation is judged to be normal according to a signal fed back by the photoelectric sensing assembly, not only the signal curve characteristic corresponding to the sample but also the signal curve characteristic corresponding to the isolation gas column are considered, and the following description specifically explains the sample sucking operation.
Referring to fig. 3, the method for confirming the sample sampling reliability of the present embodiment specifically includes steps S200 to S211.
And step S200, establishing an isolation gas column in the sampling needle under the action of a power source.
Step S201, under the action of a power source, the sampling needle performs sample suction operation from the front end needle point.
Step S203, under the action of the power source, the sample in the sampling needle is pushed forward and discharged from the front needle tip, so as to perform the sample dividing operation.
Step S205, under the action of the power source, the sample in the sampling needle is pulled back, so that the sample is at least pulled to the photoelectric sensing component arranged on the light transmission pipeline. In order to make the signal fed back by the photoelectric sensing component clearer, in an embodiment, the light-transmitting pipeline may be a transparent pipeline. Similar to the embodiment, the step S205 can be performed after the sample sucking operation is performed in the step S201 and before the sample splitting operation is performed in the step S203; or in the process of performing the sample dividing operation in step S203; step S205 may also be performed after the full sample operation is performed at step S203. When the operation of pulling back the sample in the sampling needle in step S205 is performed after the sample splitting operation in step S203, the advantage of this operation is that the influence on the sampling operation is minimal or even no influence. In one embodiment, the photoelectric sensing component is arranged at the rear end of the light transmission pipeline close to the sampling needle, so that the distance for pulling the sample is relatively reduced, and the influence on the sample is small. In one embodiment, the sample may be pulled back past the location of the photo-sensing assembly for better results.
And step S207, feeding back a signal by the photoelectric sensing assembly. In an embodiment, the photoelectric sensing component may be in an operating state during the whole sample sucking operation and the sample splitting operation, or may start to operate when the sample in the sampling needle is pulled back in step S205. When the photoelectric sensing assembly works, signals can be continuously fed back.
And S209, judging whether the sample sucking operation is normal or not according to the signal fed back by the photoelectric sensing assembly. In an embodiment, the step S207 includes determining that the operation of sucking the sample is abnormal when the curve of the signal fed back by the photoelectric sensing assembly does not include a signal curve corresponding to the sample, or does not include a signal curve corresponding to the isolated gas column, or the signal curve corresponding to the sample is abnormal, and in an embodiment, after determining that the operation of sucking the sample is abnormal, performing step S211, alarming, and further shielding the measurement result of the sample. The step S209 may further include determining that the operation of sucking the sample is normal when the curves of the signals fed back by the photoelectric sensing assembly include a normal signal curve corresponding to the sample and a signal curve corresponding to the isolated gas column, and in an embodiment, after determining that the operation of sucking the sample is normal, performing step S213 to normally output the measurement result of the sample. In this embodiment, whether the sample sucking operation is normal is determined according to the signal fed back by the photoelectric sensing assembly, and through a great deal of research and practice, the inventor finds that, in the normal sample sucking operation, when the sample is pulled to the photoelectric sensing assembly passing through the light transmitting pipe, in the corresponding signal curve fed back by the photoelectric sensing assembly, the signal curve corresponding to the sample pulled back is a curve with the length and the height within a certain range, and the signal curve corresponding to the isolated gas column is also a curve with the length and the height within a certain range, as shown in fig. 4 (a); when a signal curve corresponding to the sample is not included as shown in fig. 4(b), or a signal curve corresponding to the column of isolated gas is not included as shown in fig. 4(c), or when there is an abnormality in the signal curve corresponding to the sample as shown in fig. 4(d), it is highly likely that an abnormal sample sucking operation has occurred, in which fig. 4(b) shows a case where the signal curve corresponding to the sample is not included in the corresponding signal curve, which indicates that the sample is likely not sucked by the sampling needle, fig. 4(c) shows a case where the signal curve corresponding to the column of isolated gas is not included in the corresponding signal curve, which indicates that the column of isolated gas that the sampling needle should establish has not been established during the sample sucking operation, and there is an abnormality in the sample sucking operation, fig. 4(d) shows a case where the signal curve corresponding to the sample has an abnormality, which indicates that there is an abnormality in the sample sucking operation, for example, the sampling needle is likely to draw in air while drawing in the sample. Therefore, the case of FIG. 4(a) is judged that the pipette sample operation is normal, and the cases of FIGS. 4(b), (c) and (d) are judged that the pipette sample operation is abnormal.
EXAMPLE III
The present embodiment discloses a blood cell analyzer, as shown in fig. 5, which includes a sample sucking and separating device 100 for sucking and separating a sample, a blood sample detecting device 300, an input device 500 for inputting instructions, and a display device 700.
The sample aspirating and dispensing apparatus 100 includes a means for confirming the reliability of the sampling of the sample. Referring to fig. 6, the apparatus for confirming the reliability of sample sampling includes a sampling probe 10, a light transmission pipeline 20, a photoelectric detection assembly 30, a power source and a determination unit 50. The power source is used to provide the sampling needle 10 with power for sucking and discharging a sample, and the most common power source is a syringe, and the following description will be made by taking the syringe 40 as an example.
One end of the light transmission pipeline 20 is connected with the rear end of the sampling needle 10, and the other end is connected with the needle cylinder of the injector 40. In one embodiment, the transparent pipe 20 is a transparent pipe.
The photoelectric detection assembly 30 is disposed on the light transmission pipeline 20, and is used for feeding back a corresponding signal when the plunger core rod of the injector 40 is pulled backwards to pull the sample in the sampling needle 10 backwards to at least the photoelectric detection assembly 30 on the light transmission pipeline 20 after the sampling needle 10 performs the sample sucking operation, wherein the sample is pulled backwards to at least the photoelectric detection assembly 30, and the photoelectric detection assembly is located after the sampling needle 10 sucks the sample and before the sample is divided, or is located after the sampling needle 10 divides the sample, or is located in the sampling needle 10 divides the sample operation process. In one embodiment, the photo detection assembly 30 is disposed at the rear end of the transparent conduit 20 close to the sampling needle, so that the distance that the sample is pulled is relatively reduced, and the sample itself is not affected. In one embodiment, the sample may be pulled back past the position of the photodetecting assembly 30 for better results.
The judging unit 50 is used for judging whether the sample sucking operation is normal or not according to the signal fed back by the photoelectric detection assembly 30. In an embodiment, when the curve of the signal fed back by the photoelectric detection element 30 does not include a signal curve corresponding to the sample, or the signal curve corresponding to the sample is abnormal, the determining unit 50 determines that the operation of sucking the sample is abnormal, and the specific determining process may refer to embodiment one and fig. 2, which is not described herein again; accordingly, in an embodiment, the determining unit 50 further determines that the operation of the sample sucking is normal when the signal curve fed back by the photoelectric sensing assembly 30 includes a normal signal curve corresponding to the sample. In a preferred embodiment, considering the problem of the isolated gas column, when the curve of the signal fed back by the photoelectric detection assembly 30 does not include a signal curve corresponding to the sample, or does not include a signal curve corresponding to the isolated gas column, or the signal curve corresponding to the sample is abnormal, the determining unit 50 determines that the operation of sucking the sample is abnormal, wherein the isolated gas column is established in the sampling needle 10 before the sampling needle 10 sucks the sample; for a specific determination process, reference may be made to embodiment two and fig. 4, which is not described herein again; accordingly, in an embodiment, the determining unit 50 further determines that the sample sucking operation is normal when the curves of the signals fed back by the photoelectric sensing assembly 30 include a normal signal curve corresponding to the sample and a signal curve corresponding to the isolated gas column.
The input device 500 is used for receiving a test instruction, the sample sucking and separating device 100 sucks a sample and provides the sample to the blood detecting device 300, the blood detecting device 300 detects the sample to obtain a sample detection result, and the display device 700 displays the sample detection result. In one embodiment, when the judging unit 50 in the apparatus for confirming the reliability of sampling the sample judges that the operation of sucking the sample is abnormal, the display apparatus 700 does not display the result of the detection of the sample or displays a mark whose result is unreliable near the result of the detection of the sample. And/or, when the judging unit 50 in the device for confirming the sample sampling reliability judges that the sample sucking operation is abnormal, the device for confirming the sample sampling reliability sends an instruction to the sample sucking and separating device 100 to suck the same sample again to be provided for the blood detecting device 300, or sends an instruction to the sample sucking and separating device 100 to stop providing the sample to the blood detecting device 300.
The following describes the operation of the blood cell analyzer and the device for confirming the sample sampling reliability in this embodiment, by taking the example that the operation of pulling back the sample is performed after the sample dividing operation is completed, and whether the operation of sucking the sample is normal is determined according to the isolated gas column and the sample curve.
Before the sampling needle 10 of the blood cell analyzer is used, the sampling needle 10 is cleaned to ensure that the sample is clean or the carrying pollution of the previous sample after sampling is eliminated, before the sampling, diluent is arranged in the sampling needle 10, in order to reduce the possible dilution of the diluent to the sample, a section of air is preferably sucked before the sample is sucked, then the sampling needle 10 is moved to the sample sucking position, the plunger rod of the injector 40 is pulled backwards, the front end needle point of the sampling needle 10 is enabled to suck the sample with a preset amount, and at the moment, the air isolation between the diluent and the sample forms an isolation air column. The needle 10 is then moved to the respective partial sample positions, in which the plunger core of the syringe 40 is pushed forward in each partial sample position, so that the sample in the needle 10 is expelled from the front tip of the needle 10. When the sample dispensing operation is completed, the plunger rod of the syringe 40 is pulled back, so that the remaining sample in the sampling needle 10 is pulled back into the light transmission pipeline 20 until reaching or passing through the position of the photoelectric detection assembly 30 on the light transmission pipeline 20. The photodetecting unit 30 may be in operation during the whole of the sample sucking operation and the sample dividing operation, or may start to operate when the sample dividing operation is completed. When the photodetection module 30 is in the working state, a signal is always fed back, and the determining unit 50 is used for determining whether the sample sucking operation is normal according to the signal fed back by the photodetection module 30. When the curve of the signal fed back by the photoelectric detection assembly 30 includes a normal curve corresponding to the isolated gas column and a normal curve corresponding to the sample, as shown in fig. 4(a), it is determined that the operation of sucking the sample is normal; when the curve of the signal fed back by the photoelectric detection assembly 30 does not include a signal curve corresponding to the sample, as shown in fig. 4(b), it is determined that the operation of sucking the sample is abnormal; when the curve of the signal fed back by the photoelectric detection assembly 30 does not include the signal curve corresponding to the isolated gas column, as shown in fig. 4(c), it is also determined that the operation of sucking the sample is abnormal; when there is an abnormality in the signal curve corresponding to the sample among the curves of the signals fed back by the photodetection module 30, as shown in fig. 4(d), it is also determined that the operation of aspirating the sample is abnormal.
The application discloses a method and a device for confirming sample sampling reliability and a blood cell analyzer. The sampling needle can be applied to a sampling needle made of transparent materials such as quartz glass and hard transparent glass, can also be applied to a metal sampling needle capable of meeting the requirement of processing precision, and can judge whether the sampling needle is normal in sample sucking operation or not through a light-transmitting conduit connected with the rear end of the sampling needle and a photoelectric detection assembly arranged on the light-transmitting conduit under the condition that a sampling sample distributing valve is not needed.
The foregoing is a more detailed description of the present application in connection with specific embodiments thereof, and it is not intended that the present application be limited to the specific embodiments thereof. It will be apparent to those skilled in the art from this disclosure that many more simple derivations or substitutions can be made without departing from the inventive concepts herein.
Claims (10)
1. A method for confirming sample sampling reliability, comprising:
providing a sampling needle, wherein the rear end of the sampling needle is connected with one end of a light transmission pipeline, the other end of the light transmission pipeline is connected with a power source, and a photoelectric sensing assembly is arranged on the light transmission pipeline;
under the action of the power source, the sampling needle sucks a sample into the sampling needle from the front end of the sampling needle, and the sample sucking operation is finished;
under the action of the power source, the sample in the sampling needle is pulled back, so that the sample is at least pulled to a photoelectric sensing assembly arranged on the light transmission pipeline;
the photoelectric sensing assembly feeds back a signal;
judging whether the operation of sucking the sample is normal or not according to a signal fed back by the photoelectric sensing assembly; wherein according to the signal of photoelectric sensing subassembly feedback, judge whether inhale sample operation normal, include: and when the curve of the fed back signal does not include the signal curve corresponding to the sample or the signal curve corresponding to the sample is abnormal, judging that the operation of sucking the sample is abnormal.
2. The method of validating sample sampling reliability as claimed in claim 1, wherein:
before the sampling needle performs the sample sucking operation, an isolation gas column is also established in the sampling needle;
according to the signal of photoelectric sensing subassembly feedback, judge whether inhale sample operation normal, include: and when the curve of the fed back signal does not comprise a signal curve corresponding to the sample, or does not comprise a signal curve corresponding to the isolated gas column, or has an abnormality, judging that the operation of sucking the sample is abnormal.
3. The method of confirming sample sampling reliability of claim 1, wherein the light transmission line is a transparent line, and/or wherein the opto-electronic sensing assembly comprises a light emitter and a light receiver, the light emitter and the light receiver forming a light path through which at least a portion of the light transmission line passes.
4. The method of validating sample sampling reliability as claimed in any one of claims 1 to 3, further comprising:
under the action of the power source, part of the sample in the sampling needle is pushed forwards and discharged from the front needle tip so as to perform sample dividing operation;
the back-pulling of the sample in the sampling needle is performed after the sample sucking operation and before the sample dividing operation, or after the sample dividing operation, or during the sample dividing operation.
5. A device for confirming sample sampling reliability comprises a sampling needle and a power source, and is characterized by further comprising:
one end of the light transmission pipeline is connected with the rear end of the sampling needle, and the other end of the light transmission pipeline is connected with the power source;
the photoelectric sensing assembly is arranged on the light transmitting pipeline and used for feeding back a corresponding signal when a sample in the sampling needle is pulled backwards to at least the photoelectric sensing assembly on the light transmitting pipeline under the action of the power source after the sampling needle performs sample sucking operation, wherein the sample is pulled backwards to at least the photoelectric sensing assembly on the light transmitting pipeline and is positioned after the sampling needle sucks the sample and before the sample is divided, or is positioned after the sampling needle divides the sample, or is positioned in the sample dividing operation process of the sampling needle;
the judging unit is used for judging whether the sample sucking operation is normal or not according to the signal fed back by the photoelectric sensing assembly; and the judging unit judges that the operation of sucking the sample is abnormal when the curve of the signal fed back by the photoelectric sensing assembly does not comprise a signal curve corresponding to the sample or the signal curve corresponding to the sample is abnormal.
6. The apparatus for confirming sampling reliability of a sample according to claim 5, wherein the judging unit judges that the operation of sucking the sample is abnormal when the curve of the signal fed back from the photoelectric sensing element does not include a signal curve corresponding to the sample, does not include a signal curve corresponding to the isolated gas column, or has an abnormality in the signal curve corresponding to the sample; wherein before the sampling needle performs the sample suction operation, an isolation gas column is also established in the sampling needle.
7. The apparatus for confirming sample sampling reliability as claimed in claim 5, wherein the light transmission pipe is a transparent pipe, and/or the photoelectric sensing assembly comprises a light emitter and a light receiver, the light emitter and the light receiver form a light path, and at least one part of the light transmission pipe passes through the light path.
8. The apparatus for confirming sample sampling reliability as claimed in any one of claims 5 to 7, wherein the photoelectric sensing component is disposed at a rear end of the light transmission pipeline close to the sampling needle.
9. A blood cell analyzer, comprising a sample sucking and separating device for sucking and separating a sample, a blood sample detecting device, an input device for inputting instructions and a display device, wherein the sample sucking and separating device comprises the device for confirming the sample sampling reliability of any one of claims 5 to 8, the input device receives a test instruction, the sample sucking and separating device sucks the sample to provide for the blood detecting device, the blood detecting device detects the sample to obtain a sample detection result, and the display device displays the sample detection result.
10. The blood cell analyzer of claim 9, wherein when the means for confirming the reliability of the sampling of the sample judges that the operation of aspirating the sample is abnormal:
the display device does not display the sample detection result or displays a mark with unreliable results near the sample detection result;
and/or the presence of a gas in the gas,
the device for confirming the sample sampling reliability sends an instruction to the sample sucking and separating device to suck the same sample again so as to provide the same sample for the blood detection device, or sends an instruction to the sample sucking and separating device to stop providing the sample for the blood detection device.
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| CN110672869B (en) * | 2019-09-18 | 2023-05-02 | 东软威特曼生物科技（沈阳）有限公司 | Device and method for detecting blocking of sample needle |
| CN112485061A (en) * | 2020-10-28 | 2021-03-12 | 迈克医疗电子有限公司 | Sampling control method and sampling control device |
| CN112557685A (en) * | 2020-11-20 | 2021-03-26 | 深圳市科曼医疗设备有限公司 | Method and device for detecting sample suction of sample analyzer |
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