CN112485061A

CN112485061A - Sampling control method and sampling control device

Info

Publication number: CN112485061A
Application number: CN202011169198.8A
Authority: CN
Inventors: 杨永昌; 杨建华; 李�浩; 刘远平
Original assignee: Maccura Medical Electronics Co Ltd
Current assignee: Maccura Medical Electronics Co Ltd
Priority date: 2020-10-28
Filing date: 2020-10-28
Publication date: 2021-03-12

Abstract

The invention relates to a sampling control method and a sampling control device, wherein the sampling control method comprises the following steps: controlling a puncture needle of the sampling needle assembly to puncture a tube cap of the sample tube so as to form a sample sucking channel entering the sample tube; controlling a sample sucking needle of the sampling needle assembly to be inserted into the sample tube along a sample sucking channel to suck a sample; controlling the sample sucking needle to move out of the sample tube and move to a sample dripping position; and controlling the sample sucking needle to discharge the sample to the sample dripping position. The sampling control method simplifies the sample suction process and improves the sampling efficiency.

Description

Sampling control method and sampling control device

Technical Field

The invention relates to the technical field of medical diagnosis, in particular to a sampling control method and a sampling control device.

Background

The sample suction flow of the existing slide staining instrument for sucking a sample from a sample tube generally comprises the following steps: the puncture needle punctures the test tube cap and sucks the sample under the drive of the injection device, and then the sample in the puncture needle can be transferred into the sample dripping needle under the combined action of the injection device, the pipeline and the valve, and the sample is dripped out by the sample dripping needle, so that the subsequent operation is completed.

The liquid path in the existing sample sucking method is complex in design, samples need to be transferred back and forth continuously, the characteristics of the samples can be influenced, if the samples can be diluted, the samples are transferred by means of the injection device to move back and forth, and accumulated return errors can be generated in the using process, so that the actual sample sucking and dropping amount and the theoretical sample sucking and dropping amount have deviations respectively. In addition, the sample is transferred for multiple times, so that the required sample size is large, the blood sampling pain of special patients such as infants or anemia patients is aggravated, and the sampling efficiency is low.

Disclosure of Invention

The invention aims to provide a sampling control method and a sampling control device.

In one aspect, the present invention provides a sampling control method, including: controlling a puncture needle of the sampling needle assembly to puncture a tube cap of the sample tube so as to form a sample sucking channel entering the sample tube; controlling a sample sucking needle of the sampling needle assembly to be inserted into the sample tube along a sample sucking channel to suck a sample; controlling the sample sucking needle to move out of the sample tube and move to a sample dripping position; and controlling the sample sucking needle to discharge the sample to the sample dripping position.

According to an aspect of an embodiment of the present invention, controlling a sample aspirating needle of a sampling needle assembly to be inserted into a sample tube along a sample aspirating channel to aspirate a sample comprises: and controlling the sample sucking needle and the puncture needle to keep coaxial to suck the sample.

According to an aspect of the embodiment of the present invention, the sampling control method further includes: detecting the pressure variation of a first pipeline by a pressure sensor arranged on the first pipeline for communicating the sample sucking needle with the injection device; and if the pressure variation is larger than a preset threshold value, judging that the sample sucking needle or the first pipeline is blocked.

According to an aspect of the embodiment of the present invention, the sampling control method further includes: detecting whether a sample sucked by the sample sucking needle reaches a first target amount or not through a sample sensor which is arranged on the first pipeline and is away from the sample sucking needle by a preset distance; and if the detection result is negative, sending an alarm signal.

According to an aspect of an embodiment of the present invention, controlling the removal of the pipette tip from the sample tube further comprises: the control puncture needle and the sample sucking needle are removed from the sample tube together.

According to an aspect of the embodiment of the present invention, before controlling the sample aspirating needle to be removed from the sample tube, the method further comprises: the puncture needle is controlled to move out of the tube cap.

According to an aspect of the embodiment of the present invention, before controlling the sample sucking needle to discharge the sample to the sample dropping position, the method further includes: and controlling the cleaning device to clean the outer wall of the sample sucking needle.

According to an aspect of the embodiment of the present invention, after controlling the washing device to wash the outer wall of the sampling needle, the method further includes: and controlling the sample sucking needle to discharge the sample at the front end of the needle head.

In another aspect, the present invention provides a sampling control apparatus, including: the sampling needle assembly comprises a sample sucking needle and a puncture needle, the puncture needle is sleeved on the periphery of the sample sucking needle, the puncture needle is used for puncturing a tube cap of the sample tube to form a sample sucking channel entering the sample tube, and the sample sucking needle is used for penetrating the sample sucking channel and sucking a sample from the sample tube; and the controller is used for controlling the sampling needle assembly to move so as to enable the sample sucking needle to execute sample sucking action and sample dripping action.

According to an aspect of the embodiment of the invention, the sampling control device further comprises a cleaning swab, the puncture needle is fixedly connected with the cleaning swab, the sample sucking needle is coaxially arranged with the puncture needle, and the cleaning swab is used for cleaning the outer wall of the sample sucking needle.

According to the sampling control method and the sampling control device provided by the invention, the puncture needle of the sampling needle assembly penetrates through the tube cap of the sample tube to form the sample suction channel entering the sample tube, the sample suction needle of the sampling needle assembly is inserted into the sample tube along the sample suction channel to suck a sample, and then the sample is discharged to the sample dripping position through the sample suction needle. Compared with the prior art that the puncture needle penetrates through the tube cap of the sample tube and sucks the sample, and then the sample is transferred to the sample dripping needle for sample dripping after being transferred for multiple times, the sample sucking and sample dripping actions can be completed only by the sample sucking needle, and the puncture needle is only used for penetrating through the tube cap of the sample tube to reserve a sample sucking channel for the sample sucking needle, so that the design difficulty of the puncture needle is reduced, the sample sucking process is simplified, and the sampling efficiency is improved. In addition, the sample suction flow is simplified, so that the sample does not need to be transferred for many times, the risk of sample dilution is reduced, the sample amount required by detection is reduced, and the burden of a patient is favorably reduced.

Drawings

Features, advantages and technical effects of exemplary embodiments of the present invention will be described below with reference to the accompanying drawings. In the drawings, like parts are provided with like reference numerals. The figures are not drawn to scale.

Fig. 1 is a schematic view of an application scenario of a sampling control apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of the sampling needle assembly shown in FIG. 1;

fig. 3 is a flow chart of a sampling control method according to an embodiment of the present invention;

FIG. 4 is a schematic flow diagram of a cleaning device for a sampling needle assembly provided in accordance with an embodiment of the present invention;

FIG. 5 is a schematic view of the assembly of the cleaning tank and the cleaning swab shown in FIG. 4;

FIG. 6 is a block flow diagram of a method of cleaning a sampling needle assembly according to an embodiment of the present invention.

Detailed Description

Features and exemplary embodiments of various aspects of the present invention will be described in detail below. In the following detailed description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. It will be apparent, however, to one skilled in the art that the present invention may be practiced without some of these specific details. The following description of the embodiments is merely intended to provide a better understanding of the present invention by illustrating examples of the present invention. In the drawings and the following description, at least some well-known structures and techniques have not been shown in detail in order to avoid unnecessarily obscuring the present invention; also, the dimensions of some of the structures may be exaggerated for clarity. Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

In the conventional sample sucking method in the slide-push staining instrument, after a special puncture needle is used for puncturing a tube cap of a sample tube, a sample is sucked from the sample tube by the puncture needle, and then the sample needs to be transferred for multiple times and finally transferred into a sample dripping needle, so that the time consumption is long, and the sample can be influenced by the sample transferred back and forth, such as the sample is diluted. The puncture needle and the sample dripping needle are both in direct contact with the sample, and the risk of carrying pollution is higher. Meanwhile, the puncture needle and the sample dropping needle need to be specially cleaned, and the cleaning difficulty is high. The puncture needle needs to be specially designed to complete puncture sampling action, which has high design requirements on the puncture needle.

In addition, the puncture needle and the sample dripping needle are respectively communicated with the injection pump through pipelines, and the sample transfer process depends on the back-and-forth movement of the injection pump, so that an accumulated return error can be generated in the use process, the actual sample sucking and dripping amount and the theoretical sample sucking and dripping amount have deviation, and the sample sucking precision is influenced. Because the sample needs to be transferred for many times, the required sample size is large, and the blood sampling pain of some special patients such as infants or anemia patients is aggravated.

In order to solve the above problem, embodiments of the present invention provide a sampling control apparatus and a sampling control method.

Fig. 1 is a schematic view of an application scenario of a sampling control device according to an embodiment of the present invention for sample suction, and fig. 2 is a schematic view of a sampling needle assembly shown in fig. 1.

Referring to fig. 1 and 2 together, an embodiment of the present invention provides a sampling control device including a sampling needle assembly and a controller (not shown).

The sampling needle assembly comprises a sample sucking needle 1 and a puncture needle 2, wherein the puncture needle 2 is sleeved on the outer peripheral side of the sample sucking needle 1, the puncture needle 2 is used for puncturing a tube cap 41 of a sample tube 4 to form a sample sucking channel entering the sample tube 4, and the sample sucking needle 1 is used for penetrating the sample sucking channel and sucking a sample from the sample tube 4.

In addition, the sampling control device further includes an injection device 5 and a drive motor 6. The pipette needle 1 communicates with the injection device 5 through a first line P1, and the injection device 5 communicates with the liquid supply tank 20 through a second line P2. The injection device 5 comprises an injection tube and a rod piece arranged in the injection tube, and the driving motor 6 controls the rod piece to move back and forth in the injection tube so as to drive the sample suction needle 1 to realize automatic sample suction and sample dropping.

The controller is used for controlling the movement of the sampling needle assembly so as to enable the sample sucking needle 1 to execute sample sucking action and sample dripping action.

The sampling needle assembly is inserted into a sample tube 4, and a liquid sample is stored in the sample tube 4. The fluid sample may be, for example, a blood sample, a C-reactive protein (CRP), a push-piece sample, a glycated sample, a urine sample, a cerebrospinal fluid sample, or a pleural effusion sample, etc. For convenience of description, the embodiment of the present invention is described by taking a sample as a blood sample.

Further, after the sample is sucked from the sample tube 4 by the sample sucking needle 1, the sample remaining on the outer wall of the sample sucking needle 1 may be contaminated or oxidized before the sample sucking needle 1 is moved to the sample dropping position to perform the sample dropping, and in order to prevent the contaminated or oxidized sample remaining from dropping into the sample dropping position to affect the test result, it is necessary to clean the outer wall of the sample sucking needle 1 before the sample dropping.

Optionally, the sampling control device provided by the embodiment of the invention further includes a cleaning swab 3, the puncture needle 2 is fixedly connected with the cleaning swab 3, the sample sucking needle 1 is coaxially arranged with the puncture needle 2, and the cleaning swab 3 is used for cleaning the outer wall of the sample sucking needle 1.

Fig. 3 is a flowchart of a sampling control method according to an embodiment of the present invention. Referring to fig. 3, an embodiment of the present invention provides a sampling control method, including:

step S1: the puncture needle 2 of the control sampling needle assembly pierces the cap 41 of the sample tube 4 to form a sample aspiration path into the sample tube 4.

The puncture needle 2 moves down to puncture the cap 41 of the sample tube 4, releases the negative pressure remaining in the sample tube 4, and leaves a sample suction passage for the sample suction needle 1. The puncture needle is moved downward by a distance that the cutting edge of the needle end of the puncture needle 2 completely pierces the bottom of the cap 41 of the sample tube 4. Alternatively, after the puncture needle 2 punctures the tube cap 41, the puncture distance between the needle end cutting edge of the puncture needle 2 and the tube cap 41 is the first distance h 1. Optionally, the value range of the first distance h1 is: h1 is more than or equal to 3mm and less than or equal to 5 mm.

Step S2: the sample suction needle 1 of the sampling needle assembly is controlled to be inserted into the sample tube 4 along the sample suction channel to suck a sample.

The sample suction needle 1 can be arranged coaxially with the puncture needle 2, so that a sample sucked by the sample suction needle 1 is prevented from contacting the puncture needle 2, and the cleaning difficulty of the puncture needle 2 can be reduced. In addition, because the diameters of the sample suction needle 1 and the puncture needle 2 are smaller, and the length of the sample suction needle 1 is longer, in the process that the sample suction needle 1 is inserted into the sample tube 4 along the sample suction channel, the difficulty of controlling the sample suction needle 1 and the puncture needle 2 to be coaxial all the time is higher, so the sample suction needle 1 and the puncture needle 2 can be arranged non-coaxially, and the control difficulty of the sample suction needle 1 is reduced.

Further, after the pipette tip 1 is inserted into the sample tube 4, the distance between the tip of the pipette tip 1 and the bottom of the sample tube 4 is a second distance h 2. Optionally, the value range of the second distance h2 is: 0< h2 is less than or equal to 3 mm.

Step S3: the pipette needle 1 is controlled to move out of the sample tube 4 and move to the dripping position.

Step S4: the sample suction needle 1 is controlled to discharge the sample to the sample dropping position.

According to the sampling control method provided by the embodiment of the invention, the puncture needle 2 of the sampling needle assembly punctures the tube cap 41 of the sample tube 4 to form a sample sucking channel entering the sample tube 4, the sample sucking needle 1 of the sampling needle assembly is inserted into the sample tube 4 along the sample sucking channel to suck a sample, and then the sample is discharged to a sample dropping position through the sample sucking needle 1. Compared with the prior art that the puncture needle penetrates through the tube cap of the sample tube and sucks the sample, and then the sample is transferred to the sample dripping needle for sample dripping after being transferred for multiple times, the sample sucking and sample dripping actions can be completed only by the sample sucking needle 1, and the puncture needle 2 is only used for penetrating through the tube cap 41 of the sample tube 4 and reserving a sample sucking channel for the sample sucking needle 1, so that the design difficulty of the puncture needle 2 is reduced, the sample sucking flow is simplified, and the sampling efficiency is improved. In addition, the sample suction flow is simplified, so that the sample does not need to be transferred for many times, the risk of sample dilution is reduced, the sample amount required by detection is reduced, and the burden of a patient is favorably reduced.

The following describes in detail specific implementation steps of a sampling control method provided in an embodiment of the present invention.

As an alternative embodiment, in step S2, the method for controlling the sample suction needle 1 of the sampling needle assembly to insert into the sample tube 4 along the sample suction channel to suck the sample includes:

controlling the sample sucking needle 1 and the puncture needle 2 to keep coaxial to suck the sample.

The sample suction needle 1 and the puncture needle 2 are coaxially arranged, so that a sample adhered to the outer wall of the sample suction needle 1 can be prevented from touching the inner wall of the puncture needle 2 when the sample suction needle 1 is withdrawn from the puncture needle 2, and the steps of cleaning the puncture needle 2 can be reduced.

Further, as in the previous step S2, the method for controlling the sample suction needle 1 of the sampling needle assembly to insert into the sample tube 4 along the sample suction channel to suck the sample includes: the injection device 5, which is in communication with the pipette needle 1 through the first line P1, is controlled to aspirate a first predetermined volume of air prior to aspirating a first target amount of sample.

Specifically, the drive motor 6 drives the injection device 5 to suck air or a sample. A section of cleaning liquid supplied by the liquid supply pool 20 and air with a first preset volume are arranged in the first pipeline P1 connected between the injection device 5 and the sample suction needle 1, the cleaning liquid can provide certain pressure, the balance of the internal and external air pressures of the sample tube 4 in the sample suction process is ensured, and the accuracy of the sample suction amount is prevented from being influenced by the vacuum in the sample tube 4. And the air having the first predetermined volume separates the cleaning solution from the drawn first target amount of the sample, preventing the sample from being contaminated by the cleaning solution.

Further, the sampling control method further comprises:

step S23: the amount of change in the pressure of the first line P1 is detected by the pressure sensor 8 provided on the first line P1; if the pressure variation is larger than the predetermined threshold, it is determined that the sample suction needle 1 or the first pipeline P1 is clogged. The predetermined threshold is a predetermined threshold of pressure change to determine that a sample is drawn in the first line P1.

In the sampling control method provided by the embodiment of the invention, whether the sample sucking needle 1 works normally is detected by the pressure sensor 8 arranged on the first pipeline P1. If the pressure sensor 8 does not detect the amount of pressure change of the first line P1 or the amount of pressure change is less than or equal to a predetermined threshold, it is determined that the pipette tip 1 is operating normally.

Further, in step S3, after the control of the pipette tip 1 to move out of the sample tube 4 and before moving to the sample dropping position, the sampling control method further includes: the injection device 5 is controlled to continue to draw in the second predetermined volume of air. The second predetermined volume of air prevents the sample at the forward end of the tip of the pipette tip 1 from being diluted during subsequent cleaning of the pipette tip 1.

Further, the sampling control method provided by the embodiment of the present invention further includes: detecting whether the sample sucked by the sample sucking needle 1 reaches a first target amount by a sample sensor 7 provided on the first pipe P1 at a predetermined distance from the sample sucking needle 1; and if the detection result is negative, sending an alarm signal. If the sample sucked by the suction needle 1 does not reach the first target amount, the reasons need to be eliminated one by one. For example: the depth of insertion of the pipette needle 1 into the sample tube 4 is insufficient, the amount of sample in the sample tube 4 is insufficient, the drive motor 6 or the injection device 5 malfunctions, the first line P1 leaks, and the like.

The sample sucking needle 1 sucks a second preset volume of air to suck the sample at the front end of the needle head of the sample sucking needle 1 into the detection range of the sample sensor 7, and if the sample sensor 7 detects that the sample in the first pipeline P1 does not have enough sample, an alarm signal is sent to take corresponding treatment measures.

As an alternative embodiment, in step S3, the controlling the pipette tip 1 to move out of the sample tube 4 and to the sample dropping position further includes: the control puncture needle 2 is removed from the sample tube 4 together with the aspiration needle 1 and moved to a sample dropping position.

In order to smoothly remove the puncture needle 2 from the cap 41 of the sample tube 4, the cap 41 may be pressed by the stopper mechanism, and the puncture needle 2 may be removed from the cap 41. In this embodiment, the puncture needle 2 may be removed from the cap 41 of the sample tube 4 and the pipette needle 1 may be removed from the sample tube 4 at the same time. Then, the puncture needle 2 and the sample-aspirating needle 1 are simultaneously moved to the sample-dripping position, and the sample-aspirating needle 1 executes the sample-dripping operation.

As an alternative embodiment, before the step S3, before the step of controlling the pipette tip 1 to be removed from the sample tube 4, the method further includes: the puncture needle 2 is controlled to be removed from the cap 41.

In this embodiment, the puncture needle 2 is removed from the sample tube 4, and then the sample-sucking needle 1 is removed from the sample tube 4, so that a part of the sample on the outer wall of the sample-sucking needle 1 is "scraped" by the tube cap 41 of the sample tube 4 in advance in the process of removing the sample-sucking needle 1 from the sample tube 4, and the difficulty in cleaning the sample-sucking needle 1 is reduced.

Further, before step S4, that is, before controlling the pipette needle 1 to discharge the sample to the sample dropping position, the sampling control method provided by the embodiment of the present invention further includes:

step S33: and controlling the cleaning device to clean the outer wall of the sample sucking needle 1.

In an embodiment of the present invention, the cleaning device may include a cleaning swab 3, the puncture needle 2 is fixedly connected to the cleaning swab 3, the sample-sucking needle 1 is coaxially disposed with the puncture needle 2, and the cleaning swab 3 is used to clean an outer wall of the sample-sucking needle 1. The cleaning device can also comprise a cleaning pool for cleaning the outer wall of the sample suction needle 1. In addition, the washing device may also comprise a washing basin and a washing swab 3. When the cleaning pool and the cleaning swab 3 are independent, both can be used for cleaning the outer wall of the sample suction needle 1, or can be used for cleaning the outer wall of the sample suction needle 1 after the cleaning swab 3 and the cleaning pool are assembled into a whole, and a method for cleaning the sampling needle assembly will be described in detail later.

Further, after step S33, that is, after the outer wall of the sample aspirating needle 1 is cleaned, the sampling control method provided by the embodiment of the present invention further includes:

step S34: the sample sucking needle 1 is controlled to discharge the sample at the front end of the needle head.

Specifically, the drive motor 6 drives the injection device 5 to expel a sample at the forward end of the needle. In the process of cleaning the outer wall of the sample sucking needle 1, the sample at the front end of the needle head is possibly polluted by the cleaning liquid in the cleaning pool, so that the sample at the front end of the needle head needs to be removed to avoid influencing the detection result of the sample, and preparation is made for subsequent sample dropping.

Further, in step S4, controlling the pipette tip 1 to discharge the sample to the drop position includes: the injection device 5 is controlled to expel a second target amount of sample. The driving motor 6 drives the injection device 5 to move reversely, so that the sample sucking needle 1 discharges a second target amount of sample at a sample dropping position, wherein the second target amount is smaller than the first target amount, and the sample dropping position can be a glass slide or the like, so as to complete the sample dropping action.

Further, after the pipette tip 1 discharges a second target amount of sample, the inner wall of the pipette tip 1 needs to be cleaned. The second pipeline P2 communicated between the injection device 5 and the liquid supply pool 20 is provided with a liquid supply valve V0, the liquid supply valve V0 is opened, and the cleaning liquid in the liquid supply pool 20 is discharged from the sample suction needle 1 along the first pipeline P1 under the action of positive pressure so as to clean the inner walls of the first pipeline P1 and the sample suction needle 1. After the injection device 5 is reset, a predetermined volume of cleaning fluid is retained in the first line P1 in preparation for the next sampling, and then the fluid supply valve V0 is closed.

In the embodiment of the invention, the puncture needle 2 does not need to perform the sample dropping action, and the puncture needle 2 does not move to the sample dropping position along with the sample sucking needle 1, thereby further simplifying the sampling process.

For example, in the existing push piece dyeing instrument, a scheme that a puncture needle absorbs a sample and a sample dripping needle drips the sample is adopted, and the puncture needle and the sample dripping needle are in direct contact with the sample, so that the two needles must be completely cleaned at the same time to inhibit the generation of cross contamination, and the cleaning difficulty is high. In addition, the puncture needle and the sample dropping needle need to be cleaned separately after sample dropping is finished, the cleaning time is long, and the cleaning liquid consumption is large.

In order to solve the above problems, embodiments of the present invention provide a cleaning device and a cleaning method for a sampling needle assembly, and a detailed description is given below of a specific structure of the cleaning device and a specific step of the cleaning method for the sampling needle assembly with reference to the accompanying drawings.

Fig. 4 is a schematic view of a flow path of a cleaning device of a sampling needle assembly according to an embodiment of the present invention, and fig. 5 is a schematic view of an assembly effect of a cleaning pool and a cleaning swab shown in fig. 4.

Referring to fig. 4 and 5 together, the embodiment of the present invention provides a cleaning device for a sampling needle assembly, comprising: a wash swab 3, a wash basin 10 and a controller (not shown).

As described above, the sampling needle assembly includes the pipette needle 1 and the puncture needle 2 fitted around the outer peripheral side of the pipette needle 1, the pipette needle 1 communicates with the injection device 5 through the first pipe P1, the injection device 5 communicates with the liquid supply reservoir 20 through the second pipe P2, and the liquid supply reservoir 20 stores the cleaning liquid. The drive motor 6 controls the movement of the injection device 5 to aspirate or discharge a sample.

The cleaning swab 3 is fixedly connected with the puncture needle 2 and is used for cleaning the outer wall of the sample sucking needle 1 and the inner wall of the puncture needle 2. The cleaning tank 10 is used for cleaning the sample suction needle 1 and the puncture needle 2. The controller is used to control the cleaning swab 3 and/or the cleaning bath 10 to clean the sampling needle assembly.

Specifically, as shown in fig. 4 and 5, the cleaning tank 10 includes a first chamber 11, and a first opening 12 and a first drain port C1 which are communicated with and opposite to the first chamber 11, and a side wall 13 of the first chamber 11 is provided with a liquid supply port a, an air inlet B, and a second drain port C2 from top to bottom, respectively.

The liquid supply port a is connected to the liquid supply tank 20 through a first liquid supply pipe L1, a first valve V1 is disposed on the first liquid supply pipe L1, and the liquid supply tank 20 is used for supplying the cleaning liquid to the cleaning tank 10.

The first drain port C1 communicates with the waste liquid tank 30 through a first drain pipe W1, and a third valve V3 is provided in the first drain pipe W1. The second drain port C2 is connected to the waste liquid tank 30 via a second drain pipe W2, the second drain pipe W2 is provided with a fourth valve V4, and the waste liquid tank 30 is configured to collect waste liquid discharged from the first drain port C1 and the second drain port C2.

The air inlet B is communicated with the air supply device 40 through an air inlet pipe La, a sixth valve V6 is arranged on the air inlet pipe La, and the air supply device 40 provides compressed air with positive pressure P + for the cleaning pool 10 for drying the outer wall of the cleaned sample sucking needle 1. Meanwhile, the gas supply device 40 is also used for providing positive pressure P + or negative pressure P-for the waste liquid pool 30. A seventh valve V7 is provided on the pipe between the gas supply device 40 and the waste liquid tank 30, and the seventh valve V7 is a three-way valve and can switch between the positive pressure P + and the negative pressure P-in the waste liquid tank 30. The waste liquid tank 30 is provided with an eighth valve V8, under normal working conditions, the interior of the waste liquid tank 30 is negative pressure P-, which is convenient for the cleaning tank 10 to discharge waste liquid to the waste liquid tank 30. When the waste liquid pool 30 needs to discharge waste liquid outwards, the waste liquid pool is switched to positive pressure P + through the seventh valve V7, and the waste liquid can be discharged outwards by opening the eighth valve V8.

Further, a liquid supply valve V0 is provided in a second pipe P2 connecting the injection device 5 and the liquid supply tank 20. When the second pipeline P2 is opened, the driving motor 6 controls the injection device 5 to move, and also can discharge the cleaning liquid for cleaning the inner wall of the sample suction needle 1.

The controller controls the cleaning pool 10 to clean the outer wall of the sample sucking needle 1 and the outer wall of the puncture needle 2 by controlling the first valve V1 and the third valve V3; controlling the injection device 5 to discharge the cleaning liquid by controlling the liquid supply valve V0, and cleaning the inner wall of the sample sucking needle 1; the sixth valve V6 is controlled to control the air supply device 40 to dry the outer wall of the cleaned sample suction needle 1; after the sample sucking needle 1 is controlled to discharge the sample at the front end of the needle head, the sample remained on the needle head is removed by using negative pressure by controlling a fourth valve V4; the seventh valve V7 and the eighth valve V8 are controlled to control the waste liquid tank 30 to discharge waste liquid outwards.

Further, the compressed air provided by the air supply device 40 sweeps the needle head or the residual cleaning solution droplets on the outer wall of the sample suction needle 1 through the air inlet B of the cleaning pool 10, so as to prevent the sample from climbing along the outer wall of the sample suction needle 1 and being diluted by the cleaning solution droplets in the process of outward dripping during the subsequent sample dripping. In order to better blow off the liquid drops, optionally, a predetermined included angle θ is formed between the air inlet B of the cleaning pool 10 and the central axis of the first chamber 11, and the predetermined included angle θ has a value range of: theta is more than or equal to 30 degrees and less than or equal to 60 degrees. In the present embodiment, θ is 45 °.

In addition, the cleaning pool 10 further comprises a second chamber 21 and a second opening 22 communicated with the second chamber 21, the second chamber 21 is arranged in a crossed manner with the first chamber 11, at least part of the first chamber 11 is located in the second chamber 21, the second opening 22 and the first opening 12 are arranged in the same direction, and the second chamber 21 is provided with an overflow port D.

Optionally, the second chamber 21 is disposed at 90 ° to the first chamber 11, so as to facilitate processing and manufacturing of the cleaning tank 10. At least a portion of the first chamber 11 is located in the second chamber 21, so that the cleaning liquid overflowing from the first opening 12 of the first chamber 11 can flow into the second chamber 21 when the sampling needle assembly is cleaned, and the cleaning liquid is prevented from overflowing outwards. In addition, the second chamber 21 is provided with an overflow port D for preventing the cleaning liquid from overflowing outwardly from the second chamber 21.

As shown in FIG. 5, when the washing bath 10 and the washing swab 3 are independent from each other, both can be used for washing the outer wall of the pipette tip 1. When the cleaning tank 10 is assembled with the cleaning swab 3, it can be used for cleaning the outer wall of the pipette needle 1 and the inner wall of the puncture needle 2, and the cleaning tank 10 is also used for cleaning the outer wall of the puncture needle 2.

Referring to fig. 2 and 5, the cleaning device for a sampling needle assembly according to an embodiment of the present invention further includes a sealing member 9, the cleaning swab 3 is disposed with an annular groove 33 around the puncture needle 2, and the sealing member 9 is disposed in the annular groove 33, so that the cleaning swab 3 is hermetically connected to the cleaning pool 10. The purpose of the sealing connection is mainly to prepare the interior wall of the puncture needle 2 for subsequent cleaning of the reservoir 10, as will be described in detail later.

As shown in fig. 4 and 5, the cleaning swab 3 is provided with a through groove H extending along the axial direction thereof, and a liquid supply channel 31 and a liquid discharge channel 32 communicated with the through groove H, the liquid supply channel 31 is communicated with the liquid supply tank 20 through a second liquid supply pipe L2, the second liquid supply pipe L2 is provided with a second valve V2, the liquid discharge channel 32 is communicated with the waste liquid tank 30 through a third liquid discharge pipe W3, the third liquid discharge pipe W3 is provided with a fifth valve V5, and the fifth valve V5 is a two-way valve. The controller controls the washing swab 3 to wash the outer wall of the pipette tip 1 by controlling the second valve V2 and the fifth valve V5.

The through groove H of the cleaning swab 3 comprises a guide section H1, a cleaning section H2 and an installation section H3 which are arranged in sequence, wherein the installation section H3 is used for fixing the puncture needle 2, and as shown in figure 2, the installation section H3 fixes the puncture needle 2 through fixing glue G. The sample suction needle 1 penetrates through the through groove H, the cleaning section H2 is communicated with the liquid supply channel 31 and the liquid discharge channel 32 and used for cleaning the outer wall of the sample suction needle 1, and the guide section H1 is used for guiding the sample suction needle 1.

Since the pipette tip 1 is thin and long, in order to wash all areas where the outer wall of the pipette tip 1 directly contacts the sample, the pipette tip 1 can be moved up and down in the length direction of the through groove H of the wash swab 3, and the guide section H1 can prevent the pipette tip 1 from being displaced during the up and down movement.

In addition, an annular groove 33 is provided on the outer peripheral side of the mounting section H3, and a seal 9 is provided in the annular groove 33 to sealingly connect the cleaning swab 3 with the first opening 12 of the cleaning tank 10. Optionally, the sealing member 9 is a rubber ring, and the thickness dimension of the sealing member 9 is larger than the depth dimension of the annular groove 33, so as to improve the sealing performance between the cleaning swab 3 and the first opening 12 of the cleaning pool 10.

Referring to fig. 4 to 6, a method for cleaning a sampling needle assembly according to an embodiment of the present invention includes:

step R1: and after the sample suction needle 1 is controlled to finish sample suction from the sample tube 4, cleaning the outer wall of the sample suction needle 1.

After the sample is drawn from the sample tube 4 by the sample drawing needle 1, the sample remaining on the outer wall of the sample drawing needle 1 may be contaminated or oxidized before the sample drawing needle 1 is moved to the sample dropping position to perform the sample dropping, and in order to prevent the contaminated or oxidized sample remaining from dropping into the sample dropping position to affect the test result, it is necessary to clean the outer wall of the sample drawing needle 1 before the sample dropping. As described above, the outer wall of the sample-aspirating needle 1 may be cleaned by the cleaning swab 3 fixedly connected to the puncture needle 2, the outer wall of the sample-aspirating needle 1 may be cleaned by the cleaning tank 10, or the cleaning swab 3 and the cleaning tank 10 may be assembled together and used together to clean the outer wall of the sample-aspirating needle 1.

Step R2: after the sample is discharged to the sample dropping position by controlling the sample suction needle 1, the outer wall of the sample suction needle 1 and the inner wall of the sample suction needle 1 are cleaned again.

Taking a sample as a blood sample as an example, after the outer wall of the sample sucking needle 1 is cleaned, the sample sucking needle 1 is controlled to move to a sample dropping position, such as above a slide, and the driving motor 6 drives the injection device 5 to discharge a second target amount of sample onto the slide, so as to complete subsequent operations of smearing, dyeing and the like. At this time, the puncture needle 2 may be separated from the sample suction needle 1 and may be continuously retained in the sample tube 4 or the wash tank 10. The puncture needle 2 and the sample-sucking needle 1 can also be moved together to the dropping position and the washing tank 10, wherein when the puncture needle 2 and the sample-sucking needle 1 are moved together to the dropping position and the washing tank 10, the puncture needle 2 is always sleeved on the outer peripheral side of the sample-sucking needle 1.

After the sample is dropped from the sample suction needle 1, the sample may remain on the outer wall of the sample suction needle 1, and the outer wall of the sample suction needle 1 needs to be cleaned again. Similar to the previous method for cleaning the outer wall of the sample suction needle 1, the cleaning swab 3 fixedly connected with the puncture needle 2 can be used for cleaning the outer wall of the sample suction needle 1, the cleaning pool 10 can be used for cleaning the outer wall of the sample suction needle 1, and the cleaning swab 3 and the cleaning pool 10 can be assembled together and then used for cleaning the outer wall of the sample suction needle 1. After dropping the sample, there is generally a remaining sample in the first pipe P1 communicating with the sample-aspirating needle 1, and in order not to affect the next sampling operation, it is necessary to clean the inner wall of the sample-aspirating needle 1 and clean the remaining sample.

Because the sample sucking needle 1 is in direct contact with the sample to perform sample sucking and dropping actions, and the puncture needle 2 is used for puncturing the tube cap 41 of the sample tube 4, the puncture needle is generally not in direct contact with the sample in the sample tube 4, and only contacts the sample on the tube cap 41, the cleaning requirement on the puncture needle 2 can be reduced; the cleaning process of the sampling needle assembly is mainly used for cleaning the outer wall of the sample suction needle 1 before dropping samples, and cleaning the outer wall and the inner wall of the sample suction needle 1 after dropping samples, so that the cleaning difficulty of the whole sampling needle assembly is reduced.

According to the cleaning method of the sampling needle assembly provided by the embodiment of the invention, the sampling needle assembly comprises a sample suction needle 1 and a puncture needle 2 sleeved on the outer peripheral side of the sample suction needle 1, after sample suction is finished from a sample tube 4 by controlling the sample suction needle 1, the outer wall of the sample suction needle 1 is cleaned, and after the sample suction needle 1 is controlled to discharge a sample to a sample dropping position, the outer wall and the inner wall of the sample suction needle 1 are cleaned. The cleaning method can clean the sample sucking needle 1 independently, and because the puncture needle 2 can not directly contact with the sample in the sample tube 4 and only can contact with the sample on the tube cap 41, the cleaning requirement on the puncture needle 2 can be reduced, and the cleaning difficulty of the whole sampling needle assembly is further reduced.

The specific steps of the method for cleaning a sampling needle assembly provided by the embodiments of the present invention are described in further detail below.

As mentioned above, as an alternative embodiment, the cleaning method may be to clean the outer wall of the pipette needle 1 by using the cleaning swab 3 fixedly connected to the puncture needle 2, and the step R1 of controlling the pipette needle 1 to clean the outer wall of the pipette needle 1 after completing the pipette from the sample tube 4 includes:

step R11: the sample sucking needle 1 is controlled to move upwards, the cleaning swab 3 fixedly connected with the puncture needle 2 is controlled to clean the outer wall of the sample sucking needle 1, and the sample sucking needle 1 and the puncture needle 2 are coaxially arranged.

Alternatively, step R12: after the sample suction needle 1 is reset, the cleaning swab 3 fixedly connected with the puncture needle 2 is controlled to move downwards to clean the outer wall of the sample suction needle 1.

After the aspiration is complete, the sampling needle assembly is removed from the sample tube 4, as shown in FIG. 4. The pipette needle 1 is held in the wash swab 3 and is arranged coaxially with the puncture needle 2. Controlling the sample sucking needle 1 to move upwards or keeping the position of the sample sucking needle 1 fixed, controlling the cleaning swab 3 to drive the puncture needle 2 to move downwards, sequentially opening the fifth valve V5 and the second valve V2, namely sequentially opening the liquid discharging channel 32 and the liquid supplying channel 31 of the cleaning swab 3, spraying a cleaning liquid to the outer wall of the sample sucking needle 1 polluted by a sample at a certain speed from the liquid supplying pool 20 through the liquid supplying channel 31 of the cleaning swab 3 under the action of positive pressure, discharging a waste liquid generated in the cleaning process from the liquid discharging channel 32 to the waste liquid pool 30 to complete the cleaning of the outer wall of the sample sucking needle 1, and sequentially closing the second valve V2 and the fifth valve V5 after the cleaning is completed.

Because the sample suction needle 1 is thin and long, the method for cleaning the outer wall of the sample suction needle 1 by the swab 3 can further comprise controlling the cleaning swab 3 to clean the upper outer wall of the sample suction needle 1 during the upward movement of the sample suction needle 1, and controlling the cleaning swab 3 to move downward to clean the lower outer wall of the sample suction needle 1 after the sample suction needle 1 moves upward and returns to a position, so as to ensure that the whole area of the outer wall of the sample suction needle 1, which directly contacts with the sample, is cleaned.

As an alternative embodiment, the cleaning method may also use the cleaning pool 10 to clean the outer wall of the pipette needle 1, and then in step R1, controlling the pipette needle 1 to clean the outer wall of the pipette needle 1 after completing the pipette from the sample tube 4 includes:

step R11': controlling the sample sucking needle 1 to move to the cleaning pool 10;

step R12': the cleaning pool 10 is controlled to clean the outer wall of the sample suction needle 1.

As shown in FIG. 4, the needle of the pipette needle 1 is moved to the second drain port C2 of the washing well 10, the needle of the pipette needle 1 is at the same level as the second drain port C2, and then the third valve V3 and the first valve V1 are opened in sequence to open the first drain port C1 and the supply port A of the washing well 10, thereby completing the washing of the outer wall of the pipette needle 1.

After the pipette needle 1 finishes pipetting from the sample tube 4 as in the previous step R11 and/or R12, the puncture needle 2 is preferably fixed in position during removal from the sample tube 4, and the pipette needle 1 is moved up to wash the outer wall of the pipette needle 1 by washing the swab 3. If the process of moving up the pipette tip 1 is affected by space or mechanical structure, it is possible to wash only the upper region of the outer wall of the pipette tip 1 which directly contacts the sample. After the pipette tip 1 is moved to the wash tank 10, the lower area of the outer wall of the pipette tip 1 directly contacting the sample can be continuously washed through the previous steps R11 'to R12' to ensure that the entire area of the outer wall of the pipette tip 1 directly contacting the sample can be finally washed.

If the process of moving up the pipette tip 1 is not affected by space or mechanical structure, the entire area of the outer wall of the pipette tip 1 directly contacting the sample can be cleaned by the steps R11 and/or R12 before the pipette tip 1 moves to the cleaning bath 10. After the pipette needle 1 is moved to the washing bath 10, the steps R11 'to R12' may be omitted, and the entire area of the outer wall of the pipette needle 1 directly contacting the sample may be washed again through the steps R11 'to R12' to enhance the washing effect of the sample needle 1.

Further, after the outer wall of the sample suction needle 1 is cleaned, a cleaning liquid droplet remains on the needle head or the outer wall of the sample suction needle 1, and in order to prevent a sample from climbing along the outer wall of the sample suction needle 1 and being diluted by the cleaning liquid droplet in the process of dripping outwards during subsequent sample dripping, the sample suction needle 1 needs to be air-dried.

Therefore, before step R2, that is, before controlling the pipette tip 1 to discharge the sample to the sample dropping position, the cleaning method provided by the embodiment of the present invention further includes:

step R13: the outer wall of the sample-aspirating needle 1 after washing is dried in the washing tank 10.

Specifically, in step R13, the drying process performed on the outer wall of the sample-aspirating needle 1 after the cleaning in the cleaning tank 10 includes:

an air inlet B arranged in the cleaning pool 10 is controlled to blow air out at a preset included angle, and the sample suction needle 1 is controlled to move up and down.

As shown in fig. 4, the needle head of the sample suction needle 1 is moved to the air inlet B to ensure that the needle head of the sample suction needle 1 and the air outlet of the air inlet B are at the same horizontal line, then the sixth valve V6 is opened to allow the compressed air provided by the air supply device 40 to enter the chamber 11, purge the needle head of the sample suction needle 1, and simultaneously control the sample suction needle 1 to move up and down relative to the air inlet B to air dry the sample suction needle 1. The air inlet B and the central axis of the first chamber 11 form a preset included angle theta, so that liquid drops can be blown off better. In the present embodiment, the predetermined angle θ is in a range of 30 ° to 60 °, for example, 45 °. The air drying time can be selected to be 5s, and the sixth valve V6 is closed after the air drying is finished.

Further, after step R13, that is, after the outer wall of the sample collection needle 1 after being cleaned is dried in the cleaning pool 10, the method for cleaning a sampling needle assembly according to the embodiment of the present invention further includes:

step R14: the sample suction needle 1 is controlled to discharge the sample at the front end of the needle head and remove the sample remaining on the needle head.

As shown in fig. 4, after the outer wall of the sample-aspirating needle 1 is dried, the needle of the sample-aspirating needle 1 is moved to the second liquid-discharging port C2 to ensure that the needle of the sample-aspirating needle 1 and the second liquid-discharging port C2 are at the same horizontal line, then the driving motor 6 drives the injection device 5 to discharge the sample at the front end of the sample-aspirating needle 1, and the sample which may be contaminated or diluted at the front end of the needle is discarded to ensure that the sample used for dropping is an undiluted sample.

After the front-end sample is discarded, the sample possibly remains at the needle head of the sample sucking needle 1, the fourth valve V4 is opened at the moment to open the second liquid discharge port C2, the sample remaining at the needle head of the sample sucking needle 1 is removed by using negative pressure, and the repeatability of the subsequent dropping effect is ensured. Optionally, the negative pressure ranges from-50 kPa to-30 kPa. If a positive pressure purge process is used, the sample at the needle head may splash to other places, such as the inner and outer walls of the puncture needle 2 which is cleaned or not cleaned, the outer wall of the suction needle 1 which is cleaned, and the like, thereby affecting the subsequent dripping. The fourth valve V4 is closed after the process is completed.

And after the dropping sample is finished, cleaning the outer wall of the sample sucking needle 1 again, wherein the method for cleaning the outer wall of the sample sucking needle 1 is the same as the steps R11 and/or R12 or R11 'to R12', and the description is omitted. The method of cleaning the inner wall of the pipette tip 1 is described in detail below.

Specifically, in step R2, the step of cleaning the inner wall of the pipette tip 1 includes:

step R24: controlling the sample sucking needle 1 to move to the cleaning pool 10;

step R25: controlling an injection device 5 communicated with the sample suction needle 1 to discharge cleaning liquid so as to clean the inner wall of the sample suction needle 1.

As shown in fig. 4, the needle head of the sample aspirating needle 1 is controlled to move to the second liquid outlet C2, the needle head of the sample aspirating needle 1 and the second liquid outlet C2 are at the same horizontal line, then the fourth valve V4, the third valve V3 and the liquid supply valve V0 are sequentially opened to sequentially open the second liquid outlet C2, the first liquid outlet C1 and the second pipeline P2 between the injection device 5 and the liquid supply reservoir 20, the driving motor 6 drives the injection device 5 to reset and discharge the cleaning liquid, simultaneously the cleaning of the first pipeline P1 and the inner wall of the sample aspirating needle 1 is completed, and the liquid supply valve V0, the third valve V3 and the fourth valve V4 are sequentially closed after the cleaning is completed.

Since the puncture needle 2 does not directly contact the sample, the aforementioned method of cleaning the sampling needle assembly mainly describes a method of cleaning the pipette needle 1. The puncture needle 2 may be cleaned alone during the cleaning of the pipette needle 1 or after the cleaning of the pipette needle 1. For example, in the step R2, during the process of controlling the pipette needle 1 to discharge the sample to the sample dropping position, the puncture needle 2 can be moved to the cleaning pool 10 by the cleaning swab 3, and can also be moved to another cleaning pool to clean the inner wall and the outer wall of the puncture needle 2.

As an alternative embodiment, the puncture needle 2 may be cleaned separately while the pipetting needle 1 performs the pipetting step. Specifically, in step R2, while controlling the pipette tip 1 to discharge the sample to the sample dropping position, the method for cleaning the sampling needle assembly according to the embodiment of the present invention further includes:

step R21: controlling the cleaning swab 3 fixedly connected with the puncture needle 2 to move to the cleaning pool 10, and hermetically connecting the cleaning swab 3 with the cleaning pool 10;

step R22: the cleaning swab 3 is controlled to clean the inner wall of the puncture needle 2.

Step R23: the cleaning tank 10 is controlled to clean the outer wall of the puncture needle 2.

In the step R2, the sample is discharged to the sample dripping position by controlling the sample sucking needle 1, and the cleaning swab 3 drives the puncture needle 2 to move to the cleaning pool 10, and the puncture needle 2 is cleaned, so that the movement of the sample sucking needle 1 and the movement of the puncture needle 2 do not interfere with each other, and the efficiency of cleaning the sampling needle assembly is improved.

In addition, the sealed connection of the cleaning swab 3 and the cleaning pool 10 is mainly prepared for the subsequent cleaning of the inner wall of the puncture needle 2. As shown in fig. 4, the cleaning swab 3 carries the puncture needle 2 to the cleaning pool 10, and the cleaning swab 3 is hermetically connected with the cleaning pool 10, and the third valve V3 and the second valve V2 are sequentially opened, that is, the first drain port C1 of the cleaning pool 10 and the liquid supply channel 31 of the cleaning swab 3 are sequentially opened. The cleaning liquid supplied by the liquid supply channel 31 enters the cleaning pool 10 through the inner wall of the puncture needle 2 and washes the inner wall of the puncture needle 2 downwards under the action of the negative pressure at the bottom, and the cleaning of the inner wall of the puncture needle 2 is completed. The waste liquid generated during the process of cleaning the inner wall of the puncture needle 2 needs to be discharged to the waste liquid tank 30 through the first discharge port C1 at the bottom of the chamber 11, that is, the negative pressure required for the discharge needs to be conducted to the inner wall of the puncture needle 2. If the cleaning swab 3 is not completely sealed or the sealing effect is not good with the cleaning pool 10, the cleaning liquid entering from the liquid supply channel 31 of the cleaning swab 3 will climb up along the cleaning swab 3 to cause leakage. After the completion of the purging, the second valve V2 and the third valve V3 are closed in this order.

As shown in fig. 4, when the outer wall of the puncture needle 2 is cleaned, the cleaning swab 3 drives the puncture needle 2 to move down, so that the cutting edge of the needle end of the puncture needle 2 is lower than the liquid supply port a, and then the third valve V3 and the first valve V1 are sequentially opened to sequentially open the first liquid discharge port C1 and the liquid supply port a of the cleaning tank 10, thereby completing the cleaning of the outer wall of the puncture needle 2. After completion of the purging, the first valve V1 and the third valve V3 are closed in this order.

As an optional embodiment, in the sampling needle assembly, in a moving or static state such as a sampling process, a cleaning process, a sample dripping process and the like, the puncture needle 2 is always sleeved on the outer periphery side of the sample sucking needle 1, the sample sucking needle 1 and the puncture needle 2 are always coaxially arranged, and the sample sucking needle 1 and the puncture needle 2 can be simultaneously moved to the cleaning pool 10 for cleaning. Specifically, in step R1, after controlling the sample aspirating needle 1 to complete the sample aspirating from the sample tube 4, the method for cleaning the sampling needle assembly according to the embodiment of the present invention further includes:

step R01: controlling the puncture needle 2 to move out of the tube cap 41 of the sample tube 4;

step R02: controlling the sample sucking needle 1 to move out of the sample tube 4, wherein the sample sucking needle 1 and the puncture needle 2 are coaxially arranged;

step R03: controlling the cleaning swab 3 fixedly connected with the puncture needle 2 to move to the cleaning pool 10 along with the sample sucking needle 1, wherein the cleaning swab 3 is in sealed connection with the cleaning pool 10;

step R04: controlling the cleaning swab 3 to clean the outer wall of the sample sucking needle 1 and the inner wall of the puncture needle 2;

step R05: the cleaning tank 10 is controlled to clean the outer wall of the puncture needle 2.

In the process of removing the sampling needle assembly from the sample tube 4, the puncture needle 2 is firstly removed from the sample tube 4, and then the sample sucking needle 1 is removed from the sample tube 4, so that in the process of removing the sample sucking needle 1 from the sample tube 4, a part of a sample on the outer wall of the sample sucking needle 1 is scraped by the tube cap 41 of the sample tube 4 in advance, and the cleaning difficulty of the sample sucking needle 1 is reduced.

As shown in FIG. 4, the needle of the pipette needle 1 is moved to the second drain port C2 of the washing well 10, the needle of the pipette needle 1 is at the same level as the second drain port C2, and then the third valve V3 and the second valve V2 are opened in sequence to open the first drain port C1 of the washing well 10 and the liquid supply channel 31 of the washing swab 3.

Because the cleaning swab 3 is hermetically connected with the first opening 12 of the cleaning pool 10, the cleaning liquid supplied by the liquid supply channel 31 enters the cleaning pool 10 through the inner wall of the puncture needle 2 to complete the cleaning of the inner wall of the puncture needle 2, and the waste liquid generated by cleaning enters the bottom of the cleaning pool 10 through the gap between the inner wall of the puncture needle 2 and the outer wall of the sample suction needle 1 and is discharged to the waste liquid pool 30 under the action of negative pressure. After the completion of the purging, the second valve V2 and the third valve V3 are closed in this order.

If the process of moving up the pipette tip 1 is not affected by space or mechanical structure, the entire area of the outer wall of the pipette tip 1 directly contacting the sample can be cleaned by the steps R11 and/or R12 before the pipette tip 1 moves to the cleaning bath 10. After the sample sucking needle 1 moves to the washing tank 10, the washing swab 3 is controlled to wash the inner wall of the puncture needle 2 and simultaneously wash the outer wall of the sample sucking needle 1 again, so as to enhance the washing effect of the sample sucking needle 1.

After the cleaning swab 3 is controlled to clean the outer wall of the sample-aspirating needle 1 and the inner wall of the puncture needle 2 at the same time, the cleaning tank 10 is controlled to clean the outer wall of the puncture needle 2. This step is the same as the step R23 described above and will not be described again.

When the sample suction needle 1 is controlled to discharge the sample to the sample dripping position, the cleaning swab 3 and the puncture needle 2 can be left in the cleaning pool 10, and the sample suction needle 1 is only moved to the sample dripping position to execute the sample dripping action; the sample aspirating needle 1 and the puncture needle 2 may be moved together to the sample dropping position while keeping the same axis, and after the sample dropping operation is completed by the sample aspirating needle 1, the sample aspirating needle 1 and the puncture needle 2 may be moved back to the washing tank 10 while keeping the same axis. The inner wall and the outer wall of the puncture needle 2 are cleaned before the sample is discharged to the sample dropping position by the sample sucking needle 1, so that the sample is prevented from being dried on the inner wall or the outer wall of the puncture needle 2 after the sample dropping is finished, and the cleaning difficulty is increased.

In the embodiment, the outer wall of the sample suction needle 1 and the inner wall of the puncture needle 2 can be cleaned simultaneously, so that the time consumed for cleaning is reduced, the cleaning efficiency is improved, the consumption of cleaning liquid is reduced, and the cleaning cost is saved.

Further, in step R2, the step of cleaning the outer wall of the sampling needle 1 again includes:

step R21': controlling the sample sucking needle 1 to move to the cleaning pool 10 along the cleaning swab 3;

step R22': the cleaning pool 10 is controlled to clean the outer wall of the sample suction needle 1.

After the pipetting of the pipette tip 1 is completed, the method of cleaning the outer wall of the pipette tip 1 by the steps R21 'to R22' as described above is similar to the method of removing the pipette tip 1 from the sample tube 4 and cleaning the outer wall of the pipette tip 1 by the steps R11 'to R12' as described above, except that in this embodiment, the pipette tip 1 is controlled to move down along the cleaning swab 3 to the second liquid discharge port C2 of the cleaning bath 10 instead of the pipette tip 1 being controlled to move up along the cleaning swab 3. Then the fifth valve V5 and the second valve V2 are opened in sequence to open the liquid discharge channel 32 and the liquid supply channel 31 of the cleaning swab 3, so as to clean the outer wall of the sample suction needle 1, and the waste liquid after cleaning is discharged to the waste liquid pool 30 through the liquid discharge channel 32. After the cleaning is completed, the second valve V2 and the fifth valve V5 are closed in this order.

After the inner and outer walls of the sample suction needle 1 and the inner and outer walls of the puncture needle 2 are cleaned as before, the sampling needle assembly is reset, and the next sampling and cleaning operation of the sampling needle assembly is started.

According to the cleaning method of the sampling needle assembly provided by the embodiment of the invention, as the same sample sucking needle 1 is only adopted for sucking and dropping samples, the puncture needle 2 cannot directly contact with the samples in the sample tube 4, and the key point of cleaning is mainly the sample sucking needle 1. Compared with the cleaning method in the prior art, the cleaning difficulty of the puncture needle 2 is reduced, and the risk of carrying pollution is reduced. In addition, the cleaning method supports multiple cleaning modes, for example, the puncture needle 2 and the sample sucking needle 1 can be cleaned simultaneously, so that the cleaning time is reduced, the cleaning efficiency is improved, the consumption of cleaning liquid is reduced, and the cleaning cost is saved. For another example, the sample sucking needle 1 and the puncture needle 2 can be cleaned separately, different cleaning modes can be selected adaptively according to different application scenes, and the flexibility and the diversity of the cleaning method are improved.

While the invention has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the technical features mentioned in the embodiments can be combined in any way as long as there is no structural conflict. It is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims

1. A sampling control method, comprising:

controlling a puncture needle of a sampling needle assembly to puncture a tube cap of a sample tube so as to form a sample sucking channel entering the sample tube;

controlling a sample sucking needle of the sampling needle assembly to be inserted into the sample tube along the sample sucking channel to suck a sample;

controlling the sample sucking needle to move out of the sample tube and move to a sample dropping position;

controlling the sample sucking needle to discharge the sample to the sample dropping position.

2. The sampling control method of claim 1, wherein controlling the pipette tip of the sampling needle assembly to be inserted into the sample tube along the pipette channel to aspirate a sample comprises:

and controlling the sample sucking needle and the puncture needle to keep coaxial to suck the sample.

3. The sampling control method according to claim 1, further comprising:

detecting the pressure variation of a first pipeline of an injection device through a pressure sensor arranged on the first pipeline for communicating the sample sucking needle with the first pipeline; and if the pressure variation is larger than a preset threshold value, judging that the sample sucking needle or the first pipeline is blocked.

4. The sampling control method of claim 3, further comprising:

detecting whether the sample sucked by the sample sucking needle reaches the first target amount or not through a sample sensor which is arranged on the first pipeline and is away from the sample sucking needle by a preset distance; and if the detection result is negative, sending an alarm signal.

5. The sampling control method of claim 1, wherein the controlling the pipetting needle to move out of the sample tube and to a pipetting position further comprises:

and controlling the puncture needle and the sample sucking needle to move out of the sample tube together and move to a sample dropping position.

6. The sampling control method according to claim 1, wherein before controlling the pipette tip to move out of the sample tube, the method further comprises:

and controlling the puncture needle to move out of the tube cap.

7. The sampling control method according to any one of claims 1 to 6, wherein before controlling the pipette needle to discharge the sample to the dripping position, further comprising:

and controlling a cleaning device to clean the outer wall of the sample sucking needle.

8. The sampling control method according to claim 7, wherein after controlling the cleaning device to clean the outer wall of the pipette tip, the method further comprises:

and controlling the sample sucking needle to discharge the sample at the front end of the needle head.

9. A sampling control device, comprising:

the sampling needle assembly comprises a sample sucking needle and a puncture needle, the puncture needle is sleeved on the periphery of the sample sucking needle, the puncture needle is used for puncturing a tube cap of the sample tube to form a sample sucking channel entering the sample tube, and the sample sucking needle is used for penetrating through the sample sucking channel and sucking a sample from the sample tube;

and the controller is used for controlling the sampling needle assembly to move so as to enable the sample sucking needle to execute sample sucking action and sample dripping action.

10. The sampling control device of claim 9, further comprising a cleaning swab, wherein the puncture needle is fixedly connected with the cleaning swab, the sample suction needle is coaxially arranged with the puncture needle, and the cleaning swab is used for cleaning the outer wall of the sample suction needle.