CN107152953B - Liquid level detection method and device suitable for sample adding of glycosylated hemoglobin - Google Patents
Liquid level detection method and device suitable for sample adding of glycosylated hemoglobin Download PDFInfo
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- CN107152953B CN107152953B CN201710216701.2A CN201710216701A CN107152953B CN 107152953 B CN107152953 B CN 107152953B CN 201710216701 A CN201710216701 A CN 201710216701A CN 107152953 B CN107152953 B CN 107152953B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
- G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
- G01F23/26—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields
- G01F23/263—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1011—Control of the position or alignment of the transfer device
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N2035/1025—Fluid level sensing
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Abstract
The invention discloses a liquid level detection method and a device suitable for glycated hemoglobin sample adding, belonging to the field of liquid level detection; the method comprises the following steps: the state of synchronously acquiring optical signals, capacitance signals and resistance signals is kept while the liquid suction probe component performs downward probe movement; receiving the optical signal, the capacitance signal and the resistance signal, and instructing the imbibition probe component to stop the downward probing movement and complete imbibition according to the change condition of the signals; the change condition of the signal specifically comprises: the intensity of the optical signal is matched with a preset photoelectric signal intensity threshold value, the change value of the capacitance signal is greater than a preset capacitance detection threshold value, and an impedance loop exists; wherein, the liquid absorption probe component can be instructed to stop the liquid level downward detection movement and complete liquid absorption according to one of the obtained change conditions; the device comprises a liquid suction probe component, a sample adding arm, a motion control mechanism and a control circuit module. The invention solves the defect of low reliability of a single liquid level detection mode.
Description
Technical Field
The invention relates to the field of liquid level detection used in medical inspection analyzers, in particular to a liquid level detection method and a liquid level detection device suitable for glycated hemoglobin sample injection.
Background
At present, the liquid level detection applied to medical inspection equipment is quite wide in application, and hospitals, disease control centers, blood stations and laboratories of various grades must be equipped with various detection equipment, such as a full-automatic biochemical analyzer, a full-automatic blood coagulation analyzer, a full-automatic specific protein analyzer, a full-automatic blood cell analyzer and the like. These devices are used to suck a sample (whole blood, serum, urine, etc.) and a reagent into a reaction cell through a sample feeding needle for reaction. Because the same sample sucking needle is shared, the sample and the reagent can be subjected to cross contamination, and in order to reduce the cross contamination, the sample sucking needle is contacted with the liquid level to automatically stop downward detection and start to suck the sample or the reagent. Therefore, the accurate judgment is made through a corresponding high-sensitivity detection system at the moment when the liquid level contacts the sample sucking needle, the liquid carrying amount of the outer surface of the liquid transferring needle is reduced to the maximum extent, and the cross contamination is avoided.
the liquid level detection technology on the market at present is realized by a resistance method, a pressure method, a capacitance method and the like, and different methods have self defects: the resistance method is a traditional contact detection method used by an earlier instrument, and the method adopts two metal pole pieces to contact the liquid level to form an impedance loop so as to judge whether a sample is contacted or not, has the advantages of simplicity and easiness in implementation, but has high misjudgment rate, cannot be used when the sample with high impedance, the sample hanging drop and the temperature are lower than 0 ℃, and has no method for being suitable for detection of different types of samples; the pressure method can be seen from a small number of detection instruments, and the principle is that a fixed pressure is formed in a sample needle loop through a trace air pump string and serves as a reference pressure source, when the pressure is instantly increased after the pressure is contacted with a liquid level, the pressure is transmitted to a corresponding amplification processing circuit through a pressure sensor, so that the movement of a sample adding needle is controlled, and the pressure method has the defects of complex realization, high influence caused by pipeline sealing and foreign matters in an inner arm of a needle hole, high failure rate and the like; the capacitance method is the most popular method at present, the ratio of the method used by the instruments on the market is more than 90%, the detection principle is that a fixed reference field capacitance is arranged between a sample sucking needle and a sample (or a reagent), a sudden change capacitance is generated at the moment when the sample sucking needle contacts the sample, the formation of a liquid level detection signal is finally triggered through a series of circuit conversion and signal processing, the downward detection of the sample adding needle is stopped through controlling a movement mechanism, the method is relatively simple to realize, the failure rate is lower than that of other two methods, but the capacitance method can cause misjudgment under the influences of distributed capacitance of the detected sample environment, the self capacitance of the sample, electromagnetic interference, even environment temperature and the like, so that a liquid level detection system transmits wrong instructions to the main control circuit, the sample sucking error is caused, the cross contamination of the sample and the reagent is caused, and the sample testing error can be caused when the method is serious, misleading a doctor to take a medication may have serious consequences for the patient.
disclosure of Invention
in view of the above-mentioned drawbacks of the prior art, an object of the present invention is to provide a method and an apparatus for detecting a liquid level suitable for loading glycated hemoglobin, which have multiple liquid level detection modes, so as to solve the problem of low reliability of a single liquid level detection mode.
The purpose of the invention is realized by the following technical scheme:
In a first aspect, the present invention provides a liquid level detecting device suitable for loading glycated hemoglobin, comprising: the device comprises a liquid suction probe component, a sample adding arm, a motion control mechanism and a control circuit module; wherein the content of the first and second substances,
The liquid suction probe component is used for collecting and outputting capacitance, resistance and optical signals and sucking a sample;
The sample adding arm is connected with the imbibing probe component and is used for driving the imbibing probe component to complete sample absorption;
The motion control mechanism is connected with the sample adding arm and is used for controlling the motion of the sample adding arm in the horizontal direction and the vertical direction;
And the control circuit module is used for receiving the capacitance, the resistance and the optical signal output by the imbibing probe component and controlling the motion control mechanism to drive the sample adding arm to move in the horizontal direction and the vertical direction.
In one specific form of the embodiment, the liquid suction probe component includes a liquid suction needle tube, and a capacitance detection part, an impedance part and a photoelectric detection part which are arranged on the liquid suction needle tube, and the capacitance detection part, the impedance part and the photoelectric detection part are sequentially away from the liquid suction port end of the liquid suction needle tube along the longitudinal axis direction of the liquid suction needle tube.
in a specific form of the embodiment, the capacitance detection part is a capacitive sample suction nozzle of a liquid suction needle tube.
In a specific form of the embodiment, the impedance portion is disposed between the capacitance detecting portion and the photoelectric detecting portion, and is a metal sleeve surrounding the liquid suction needle tube.
In one specific form of the embodiment, the photoelectric detection part includes a cylindrical photoelectric shielding cover and a photoelectric sensor arranged at the bottom of the photoelectric shielding cover, and the liquid suction needle tube penetrates through the photoelectric shielding cover along the longitudinal axis direction and the liquid suction port end part is exposed.
As a particular form of embodiment, the motion control mechanism comprises a horizontal motion control and a vertical motion control.
In a specific form of the embodiment, the control circuit module includes a current-to-voltage conversion circuit, an operational amplifier, a voltage comparator, a microprocessor, and a rc receiving oscillation finishing circuit.
In a second aspect, the present invention provides a liquid level detection method suitable for glycated hemoglobin sample application, which is applied to a liquid level detection device of a full-automatic specific protein analyzer, and comprises the following steps:
S101, keeping the state of synchronously acquiring optical signals, capacitance signals and resistance signals while the liquid suction probe component performs downward probe movement;
S102, receiving the optical signal, the capacitance signal and the resistance signal, and instructing the liquid suction probe component to stop the downward detection movement according to the change condition of the signals to finish liquid suction; the change condition of the signal specifically comprises: the intensity of the optical signal is matched with a preset photoelectric signal intensity threshold value, the change value of the capacitance signal is greater than a preset capacitance detection threshold value, and an impedance loop exists;
and the liquid absorption probe component can be instructed to stop the liquid level downward detection movement and complete liquid absorption according to one of the change conditions acquired firstly.
As a specific form of the embodiment, in S102, the setting of the threshold of the intensity of the optical signal is specifically as follows: the height of the imbibition probe component from the liquid level is in corresponding relation with the optical signal intensity, if the imbibition probe component stops at a preset height from the liquid level, the imbibition is stopped, and the imbibition is completed, so that the optical signal intensity corresponding to the preset height is the optical signal intensity threshold value.
as a specific form of the embodiment, in S102, the setting of the capacitance detection threshold specifically is: the capacitance values to be absorbed in different volumes are different, the superposed capacitance values generated instantly after the sample absorbing nozzle of the liquid absorbing probe component is contacted with the sample absorbing nozzle are different, and correspondingly, the oscillation frequencies corresponding to the superposed capacitance values are different; and if the liquid absorption probe part stops detecting at the moment that the sample absorption nozzle of the liquid absorption probe part is contacted with the sample to be absorbed to finish liquid absorption, the change value of the oscillation frequency corresponding to the detected superposition capacitance value and the blank reference frequency is the capacitance detection threshold value.
Compared with the prior art, the embodiment of the invention has the following beneficial effects:
the liquid level detection method adopts a non-single liquid level detection mode, can ensure that the phenomena of cross contamination, sample leakage, aspiration and the like caused when a full-automatic specific protein analyzer sucks a sample are avoided, ensures the accuracy of sample addition, and provides guarantee for the sample addition detection of the analyzer.
drawings
FIG. 1 is a schematic structural diagram of a liquid level detection device provided in an embodiment of the present invention;
FIG. 2 is a schematic diagram of the overall structure of a fully automatic specific protein analyzer using a liquid level detection device provided by an embodiment of the present invention;
FIG. 3 is an enlarged partial schematic view of a pipetting probe assembly of the fluid level sensing apparatus provided in accordance with an embodiment of the invention;
The device comprises a liquid absorption probe component 10, a liquid absorption needle tube 11, a capacitance detection part 12, an impedance part 13, a photoelectric detection part 14, a photoelectric sensor 141, a photoelectric emission end 1411, a photoelectric receiving end 1412, a photoelectric shielding cover 142, a sample adding arm 20, a motion control mechanism 30, a horizontal motion control part 31, a vertical motion control part 32, a control circuit module 40, a sample reagent disk 50 and a sample reagent bottle 60.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
In order to facilitate an understanding of the invention, the invention is described in more detail below with reference to the accompanying drawings and specific examples. It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for descriptive purposes only.
unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 and 3, an embodiment of the invention provides a liquid level detection device 100 suitable for glycated hemoglobin sample application, including: a pipette probe member 10, a sample application arm 20, a motion control mechanism 30, and a control circuit module 40; wherein the content of the first and second substances,
The liquid suction probe component 10 is used for collecting and outputting capacitance signals, resistance signals and optical signals and sucking a sample;
The sample adding arm 20 is connected with the imbibing probe component 10 and is used for driving the imbibing probe component 10 to complete sample absorption;
The motion control mechanism 30 is connected to the sample-adding arm 20, and is configured to control the motion of the sample-adding arm 20 in the horizontal direction and the vertical direction;
the control circuit module 40 is configured to receive the capacitance signal, the resistance signal, and the optical signal output by the pipetting probe assembly 10, and accordingly control the motion control mechanism 30 to move in the horizontal direction and the vertical direction.
the liquid suction probe component 10 comprises a liquid suction needle tube 11, and a capacitance detection part 12, an impedance part 13 and a photoelectric detection part 14 which are arranged on the liquid suction needle tube 11, wherein the capacitance detection part 12, the impedance part 13 and the photoelectric detection part 14 are sequentially away from the liquid suction end of the liquid suction needle tube 11 along the longitudinal axis direction of the liquid suction needle tube 11.
in some embodiments, the capacitance detecting unit 12 is a capacitive sample nozzle of the pipette needle 11.
in some embodiments, the sample suction nozzle is made of an insulating material, such as rubber, plastic, glass, ceramic, and the like.
In some embodiments, the impedance portion 13 is disposed between the capacitance detecting portion 12 and the photoelectric detecting portion 14, and is a metal sheath surrounding the pipette needle 11.
In some embodiments, the photodetection part 14 includes a cylindrical photoelectric shielding case 142 and a photosensor 141 disposed at the bottom of the photoelectric shielding case 142, and the pipette needle 11 penetrates the photoelectric shielding case 142 along the longitudinal axis direction and the end part of the pipette port is exposed.
In some embodiments, the optoelectronic shielding can 142 is made of an insulating material, such as rubber, plastic, glass, ceramic, or the like.
In some embodiments, the photosensor 141 is located at a distance of 10-20 mm from the pipette tip of the pipetting probe component 10.
In some embodiments, the photosensor 141 includes a photo emitter 1411 and a photo receiver 1412.
in some embodiments, the motion control mechanism 30 includes a horizontal motion control 31 and a vertical motion control 32.
the control circuit module 40 includes a current-to-voltage conversion circuit, an operational amplifier, a voltage comparator, a microprocessor, and the like.
The fully automatic specific protein analyzer equipped with the liquid level detection device 100 suitable for glycated hemoglobin sample application according to the embodiment of the present invention is shown in fig. 2. In order to facilitate the technical solution of the present invention to be understood by those skilled in the art, the inventor explains the operation mode of the present invention as follows:
the control circuit module 40 includes a current-voltage conversion circuit, an operational amplifier, a voltage comparator, a microprocessor, etc. for controlling the motion control mechanism 30 according to the capacitance signal, the resistance signal, and the optical signal command outputted from the pipetting probe unit 10, and driving the pipetting probe unit 10 to the sample reagent tray 50 to aspirate the sample from the sample reagent bottle 60. When the vertical motion control part 32 drives the liquid suction probe part 10 to move downwards, the photoelectric emission end 1411 of the photoelectric sensor 141 continuously detects the distance between the sample suction nozzle of the liquid suction needle tube 11 and the liquid level to be sucked by emitting infrared light, the photoelectric receiving end 1412 receives different light source signal intensities by detecting the distance between the liquid level to be sucked and the sample suction nozzle, judges the height of the liquid level to be sucked by converting current and voltage from light to electricity, amplifying the signal of an operational amplifier, comparing a voltage comparator and comprehensively analyzing a microprocessor, controls the motion control mechanism 30 by the control circuit module 40, and immediately stops the downward detection action of the liquid suction probe part 10 after the sample suction nozzle contacts the liquid level to complete liquid level detection.
the principle of action of the photosensor 141 is: the photoelectric sensor 141 is a conversion device from light to electric signals, the photoelectric emitting end 1411 continuously emits infrared light during working, when the photoelectric sensor 141 approaches the liquid surface and the intensities of the light reflected to the photoelectric receiving end 1412 are different, the photoelectric signals are amplified by the circuit and input to the A/D conversion control IC, and the photoelectric signal thresholds of different distances and different liquids are set in a program, so that the pairing relation is formed by the strength thresholds of the electric signals. This is generally achievable by a person skilled in the art.
At the moment that the liquid suction probe component 10 contacts the liquid level, the liquid suction probe component 10 and the liquid level to be sucked form a superposed capacitance to generate a capacitance signal. The capacitance signal is instantaneously transmitted to the rc receiving oscillating and sorting circuit of the control circuit module 40, the oscillation frequency is greatly changed after contacting the liquid level, whether to contact the liquid level is determined according to the variation of the oscillation frequency by comparing with the oscillation frequency of the empty base value, and the capacitance formed by different amounts of the liquid to be absorbed is also greatly different, so that different thresholds are required to be set, for example, the minimum liquid amount allowed for full-automatic specific protein liquid level detection is 100uL, the oscillation frequency generated when the liquid absorption probe component 10 contacts a sample at the corresponding position of 100uL is compared with the empty base frequency, and the variation value is calculated, that is, the minimum sample absorption amount detection threshold is obtained.
when the photoelectric detection part 14 and the capacitance detection part 12 lose operation ability, the liquid absorption probe component 10 continues to probe under the driving of the sample adding arm 20, and forms an impedance loop with the impedance part 13 after the liquid surface contacts the impedance part 13, so as to excite the resistance-capacitance detection circuit, generate a resistance signal, amplify and compare the resistance signal, input the resistance signal to the micro-processing chip for judgment, and control the motion control mechanism 30 to stop running by the control circuit module 40 according to the resistance signal.
The embodiment of the invention also provides a liquid level detection method suitable for glycated hemoglobin sample adding, which is applied to a liquid level detection device of a full-automatic specific protein analyzer and comprises the following steps:
S101, keeping the state of synchronously acquiring optical signals, capacitance signals and resistance signals while the liquid suction probe component performs downward probe movement;
S102, receiving the optical signal, the capacitance signal and the resistance signal, and instructing the liquid suction probe component to stop the downward detection movement according to the change condition of the signals to finish liquid suction; the change condition of the signal specifically comprises: the intensity of the optical signal is matched with a preset photoelectric signal intensity threshold value, the change value of the capacitance signal is greater than a preset capacitance detection threshold value, and an impedance loop exists;
and the liquid absorption probe component can be instructed to stop the liquid level downward detection movement and complete liquid absorption according to one of the change conditions acquired firstly.
In the above S102, the setting of the threshold of the intensity of the optical signal is specifically: the height of the imbibition probe component from the liquid level is in corresponding relation with the optical signal intensity, if the imbibition probe component stops at a preset height from the liquid level, the imbibition is stopped, and the imbibition is completed, so that the optical signal intensity corresponding to the preset height is the optical signal intensity threshold value.
in the above S102, the setting of the capacitance detection threshold specifically includes: the capacitance values to be absorbed in different volumes are different, the superposed capacitance values generated instantly after the sample absorbing nozzle of the liquid absorbing probe component is contacted with the sample absorbing nozzle are different, and correspondingly, the oscillation frequencies corresponding to the superposed capacitance values are different; and if the liquid absorption probe part stops detecting at the moment that the sample absorption nozzle of the liquid absorption probe part is contacted with the sample to be absorbed to finish liquid absorption, the change value of the oscillation frequency corresponding to the detected superposition capacitance value and the blank reference frequency is the capacitance detection threshold value.
The embodiment combines the resistance method mode, the capacitance method mode and the photoelectric method mode, and is applied to one set of liquid level detection device, so that the defect of low reliability of a single detection mode is overcome. Although the prior art only uses the resistive method mode, the capacitive method mode and the photoelectric method mode, the technical scheme of the embodiment of the invention combining the modes still has creativity, and the reason is that:
1. Although photoelectric detection already exists, the photoelectric sensor has a large volume, cannot be embedded into the sample adding needle and cannot be realized in the production process, so that the technology is not used for liquid level detection all the time; in the embodiment of the invention, the photoelectric sensor 141 is successfully combined with the liquid suction needle tube 11 by using the structures such as the photoelectric shielding cover 142, and the like, so that the application of the photoelectric sensor in liquid level detection is finally realized;
2. The photoelectric sensor is easily interfered by peripheral external stray light, even the operator walks and brings great influence, the technologies cannot overcome, the photoelectric transmitting end 1411 and the photoelectric receiving end 1412 are controlled by the micro-control chip in the embodiment, pulse width square waves conforming to pulses and different duty ratios are output to drive the photoelectric transmitting end 1411, and when a received pulse signal is returned to conform to the transmitted pulses and pulse width signals, the control system confirms that the liquid detection range is not the external interference, namely the liquid level detection device in the embodiment of the invention has the functions of judging and discharging the external interference;
3. because different samples and reagents have different colors and different reflection efficiencies, the judgment is difficult; therefore, in the implementation process of the detection method in the embodiment, different feedback signal intensity thresholds are set in the full-automatic specific protein analyzer according to different samples, and the distance of the sample is judged according to technical parameters such as pulse counting and pulse width, so that the technical difficulties are overcome.
It should be noted that the description of the present invention and the accompanying drawings illustrate preferred embodiments of the present invention, but the present invention may be embodied in many different forms and is not limited to the embodiments described in the present specification, which are provided as additional limitations to the present invention and to provide a more thorough understanding of the present disclosure. Moreover, the above technical features are combined with each other to form various embodiments which are not listed above, and all the embodiments are regarded as the scope of the present invention described in the specification; further, modifications and variations will occur to those skilled in the art in light of the foregoing description, and it is intended to cover all such modifications and variations as fall within the true spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. A liquid level detection device (100) suitable for loading glycated hemoglobin, comprising: a liquid suction probe member (10), a sample addition arm (20), a motion control mechanism (30), and a control circuit module (40); wherein the content of the first and second substances,
The imbibition probe component (10) comprises an imbibition needle tube (11), and a capacitance detection part (12), an impedance part (13) and a photoelectric detection part (14) which are arranged on the imbibition needle tube (11), wherein the capacitance detection part (12), the impedance part (13) and the photoelectric detection part (14) are sequentially far away from the imbibition end of the imbibition needle tube (11) along the longitudinal axis direction of the imbibition needle tube (11);
the liquid suction probe component (10) is used for collecting and outputting capacitance, resistance and optical signals and sucking a sample;
The sample adding arm (20) is connected with the imbibing probe component (10) and is used for driving the imbibing probe component (10) to finish sample absorption;
the motion control mechanism (30) is connected with the sample adding arm (20) and is used for controlling the motion of the sample adding arm (20) in the horizontal direction and the vertical direction;
the control circuit module (40) is used for receiving a capacitance signal output by the capacitance detection part (12), a resistance signal output by the impedance part (13) and an optical signal output by the photoelectric detection part (14), and controlling the motion control mechanism (30) to drive the sample adding arm (20) to move in the horizontal direction and the vertical direction according to the capacitance signal, the control circuit module is used for keeping the state of synchronously acquiring the optical signal, the capacitance signal and the resistance signal while the liquid suction probe component does downward movement, and stopping downward movement and completing liquid suction according to one of the signal change conditions acquired firstly;
The change condition of the signal specifically comprises: the intensity of the optical signal is matched with a preset threshold value of the intensity of the optical signal, the change value of the capacitance signal is larger than a preset threshold value of a capacitance detection threshold value, and an impedance loop exists.
2. The liquid level detecting device (100) according to claim 1, wherein the capacitance detecting unit (12) is a capacitive sample nozzle of a pipette needle (11).
3. The apparatus (100) for detecting the liquid level according to claim 1, wherein the impedance unit (13) is a metal sheath surrounding the pipette needle (11) and disposed between the capacitance detecting unit (12) and the photodetection unit (14).
4. The liquid level detecting device (100) according to claim 1, wherein the photodetector (14) comprises a cylindrical photodetector shield (142) and a photosensor (141) disposed at a bottom of the photodetector shield (142), and the pipette needle (11) penetrates the photodetector shield (142) in a longitudinal direction with a pipette tip portion exposed.
5. the liquid level detecting device (100) for loading of glycated hemoglobin according to claim 1, wherein the motion control mechanism (30) comprises a horizontal motion control member (31) and a vertical motion control member (32).
6. The liquid level detecting device (100) for loading glycated hemoglobin according to claim 1, wherein the control circuit module (40) comprises a current-to-voltage conversion circuit, an operational amplifier, a voltage comparator, a microprocessor, and a resistance-capacitance receiving oscillation-trimming circuit.
7. a method for detecting a liquid level suitable for loading glycated hemoglobin, which is applied to the liquid level detecting device (100) suitable for loading glycated hemoglobin according to any one of claims 1 to 6, the method comprising the steps of:
s101, keeping the state of synchronously acquiring optical signals, capacitance signals and resistance signals while the liquid suction probe component performs downward probe movement;
S102, receiving the optical signal, the capacitance signal and the resistance signal, and instructing the liquid suction probe component to stop the downward detection movement according to the change condition of the signals to finish liquid suction; the change condition of the signal specifically comprises: the intensity of the optical signal is matched with a preset intensity threshold of the optical signal, the change value of the capacitance signal is greater than a preset capacitance detection threshold, and an impedance loop exists;
And the liquid absorption probe component can be instructed to stop the liquid level downward detection movement and complete liquid absorption according to one of the change conditions acquired firstly.
8. the method for detecting the liquid level according to claim 7, wherein in step S102, the threshold of the intensity of the optical signal is specifically set as follows: the height of the imbibition probe component from the liquid level has a corresponding relation with the optical signal intensity, if the imbibition probe component stops at a preset height from the liquid level, the imbibition is stopped, and the imbibition is completed, so that the optical signal intensity corresponding to the preset height is the optical signal intensity threshold.
9. The method for detecting the liquid level according to claim 7, wherein in step S102, the capacitance detection threshold is specifically set as follows: the capacitance values to be absorbed are different in different volumes, the superposed capacitance values generated at the moment when the sample absorbing nozzle of the liquid absorbing probe component is contacted with the sample absorbing nozzle are different, and correspondingly, the oscillation frequencies corresponding to the superposed capacitance values are different; and if the liquid absorption probe part stops detecting at the moment that the sample absorption nozzle of the liquid absorption probe part is contacted with the sample to be absorbed to finish liquid absorption, the change value of the oscillation frequency corresponding to the detected superposition capacitance value and the blank reference frequency is the capacitance detection threshold value.
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| CN1169779A (en) * | 1995-04-11 | 1998-01-07 | 准确系统科学株式会社 | Liquid suction examination method and dispensation apparatus drive-controlled by the same |
| CN102478417A (en) * | 2010-11-25 | 2012-05-30 | 上海安彩智能技术有限公司 | Liquid level detection device for sample addition system |
| CN203274849U (en) * | 2013-05-28 | 2013-11-06 | 深圳市希莱恒医用电子有限公司 | Liquid level detection device applied to medical detection instrument |
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| JPS62163968A (en) * | 1986-01-16 | 1987-07-20 | Nitsuteku:Kk | Pipet for confirming liquid amount and liquid amount confirming method and apparatus using said pipet |
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2017
- 2017-04-01 CN CN201710216701.2A patent/CN107152953B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1169779A (en) * | 1995-04-11 | 1998-01-07 | 准确系统科学株式会社 | Liquid suction examination method and dispensation apparatus drive-controlled by the same |
| CN102478417A (en) * | 2010-11-25 | 2012-05-30 | 上海安彩智能技术有限公司 | Liquid level detection device for sample addition system |
| CN203274849U (en) * | 2013-05-28 | 2013-11-06 | 深圳市希莱恒医用电子有限公司 | Liquid level detection device applied to medical detection instrument |
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| CN107152953A (en) | 2017-09-12 |
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