CN105784684A - Electrochemiluminescence detection method and device through paper detection cell - Google Patents
Electrochemiluminescence detection method and device through paper detection cell Download PDFInfo
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- CN105784684A CN105784684A CN201610124405.5A CN201610124405A CN105784684A CN 105784684 A CN105784684 A CN 105784684A CN 201610124405 A CN201610124405 A CN 201610124405A CN 105784684 A CN105784684 A CN 105784684A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Abstract
The invention discloses an electrochemiluminescence detection method and device through a paper detection cell.The method comprises the steps that a reaction mixture containing a substance to be detected, an electrochemiluminescence active substance and a co-reactant is prepared; a printing three-electrode chip and filter paper are sequentially placed on a tray of a paper chip electrochemiluminescence detector, and the reaction mixture is placed on the filter paper; a photoelectric multiplier tube of the paper chip electrochemiluminescence detector is adopted for carrying out electrochemiluminescence detecting, and the electrochemiluminescence intensity of the reaction mixture is recorded in real time; according to the recorded electrochemiluminescence intensity, a standard curve method is adopted for calculating the concentration of the substance to be detected.The device does not need to be used in cooperation with a special reagent, and has universality; the filter paper detection cell is adopted to form a paper lab, and the cost is low; the paper lab based on the paper detection cell is adopted for carrying out electrochemiluminescence detecting, the reaction efficiency is higher, and the detection cell does not need to be cleaned.The device can be widely applied to the field of scientific instruments.
Description
Technical field
The present invention relates to scientific instrument field, especially one paper detection cell electrochemical luminous detection method and device.
Background technology
Electrochemiluminescence (Electrochemiluminescence, ECL) is a kind of chemiluminescence phenomenon caused by electrochemical reaction at electrode surface.Electrochemiluminescence immune detection is that the shiner being marked on antibody by detection realizes.
Electrochemiluminescence detection does not need external light source, eliminate the veiling glare in common fluorescent analysis and cause the feature of fluctuation because light source luminescent is unstable, thus reducing noise, improve signal to noise ratio, add sensitive photoelectric detecting technology, this method has the advantages such as highly sensitive, range of linearity width, instrument are simple, is relatively more suitable for the quick detection of the complex component such as biomedicine, environmental science.The analyte (such as antibody, antigen, hormone etc.) comprised in body fluid (such as blood, urine, saliva etc.) in clinical medicine domain especially, its concentration shows the index of body somewhere health or pathological changes often, and it is very important for carrying out analyzing accurately to it.And electrochemiluminescence detection technique, exactly it is especially suitable for the trace detection to material (analyte) concentration comprised in fluid sample.
Present hospitals adopts Roche fully automatic electric chemical illumination immunity analysis instrument to carry out electrochemiluminescence detection mostly, but Roche fully automatic electric chemical illumination immunity analysis instrument also exists following defect or deficiency:
A. it is the special inspecting equipment of immune detection, it is necessary to support the use with special reagent, do not possess universality, it is difficult to be applied to the fields such as gene test.
B. have employed rustless steel detection cell, it is necessary to by working electrode, construct in rustless steel detection cell to electrode and these 3 electrodes of reference electrode, the consumption of sample and reagent is big, relatively costly.
C. have employed flow injection detection technique, luminescent substance and interelectrode reaction to be undertaken by Brownian movement, reaction efficiency is low, and just can react after needing rustless steel detection cell is carried out next time, easy not.
Summary of the invention
For solving above-mentioned technical problem, it is an object of the invention to: provide a kind of have universality, cost is low, reaction efficiency is high and without clean, paper detection cell electrochemical luminous detection method.
Another object of the present invention is to: provide a kind of have universality, cost is low, reaction efficiency is high and without clean, paper detection cell electrochemical light-emitting detector.
The technical solution used in the present invention is:
A kind of paper detection cell electrochemical luminous detection method, comprises the following steps:
S1, the preparation reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant；
S2, on the pallet of paper chip Electrochemial luminescence detecting instrument, it is sequentially placed printing three electrode chips and filter paper, and reactant mixture is placed on filter paper；
S3, adopt paper chip Electrochemial luminescence detecting instrument photomultiplier tube reactant mixture is carried out electrochemiluminescence detection the electrochemiluminescence intensity of real time record reactant mixture；
S4, according to record electrochemiluminescence intensity adopt standard curve method, calculate the concentration of determinand.
Further, described step S1 particularly as follows:
Adopt double-antibody method to obtain the reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant, and removed the material failing to combine by magnetic separation method.
Further, described step S2 includes:
S21, press the button that opens a position of paper chip Electrochemial luminescence detecting instrument, make pallet stretch out from Hatch Opening；
S22, successively placement printing three electrode chips and filter paper on the pallet of paper chip Electrochemial luminescence detecting instrument, and reactant mixture is placed on filter paper；
S23, press storehouse, the pass button of paper chip Electrochemial luminescence detecting instrument, make in tray retracted Hatch Opening and close storehouse.
Further, described step S3 includes:
S31, the electrode printing three electrode chips is applied voltage, make reactant mixture luminous；
The luminous intensity of reactant mixture is detected in real time by S32, employing cyclic voltammetry by photomultiplier tube；
S33, real time record reactant mixture luminous intensity.
Further, described step S32, when the luminous intensity of reactant mixture being detected in real time by photomultiplier tube, by being placed in the light-receiving window of photomultiplier tube by the intermediate reaction region printing three electrode chips on pallet, is taked lucifuge measure and makes the mode of photomultiplier tube and the intermediate reaction region accurate contraposition of printing three electrode chips catch faint optical signal.
Further, the scanning speed of described cyclic voltammetry is 0.1 volt/second, and the high pressure of described photomultiplier tube is 1000 volts.
Further, described filter paper is the filter paper of 6 mm dias.
What the present invention taked another solution is that
A kind of paper detection cell electrochemical light-emitting detector, including with lower module:
Preparation module, for preparing the reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant；
Placement module, for being sequentially placed printing three electrode chips and filter paper on the pallet of paper chip Electrochemial luminescence detecting instrument, and is placed in reactant mixture on filter paper；
Electrochemiluminescence detection and logging modle, for adopting the photomultiplier tube of paper chip Electrochemial luminescence detecting instrument that reactant mixture carries out electrochemiluminescence detection the electrochemiluminescence intensity of real time record reactant mixture；
Concentration calculation module, adopts standard curve method for the electrochemiluminescence intensity according to record, calculates the concentration of determinand；
Described preparation module passes sequentially through placement module and electrochemiluminescence detects with logging modle and then is connected with the input of concentration calculation module.
Further, described placement module includes:
Open a position unit, for pressing the button that opens a position of paper chip Electrochemial luminescence detecting instrument, makes pallet stretch out from Hatch Opening；
Placement unit, for placing printing three electrode chips and filter paper successively on the pallet of paper chip Electrochemial luminescence detecting instrument, and is placed in reactant mixture on filter paper；
Pass warehouse receipt unit, for pressing storehouse, the pass button of paper chip Electrochemial luminescence detecting instrument, makes in tray retracted Hatch Opening and closes storehouse；
The outfan of described preparation module pass sequentially through the unit that opens a position, placement unit, pass warehouse receipt unit and then and electrochemiluminescence detection be connected with the input of logging modle.
Further, the detection of described electrochemiluminescence includes with logging modle:
Pressure luminescence unit, for the electrode printing three electrode chips is applied voltage, makes reactant mixture luminous；
Detect unit in real time, for adopting cyclic voltammetry by photomultiplier tube, the luminous intensity of reactant mixture to be detected in real time；
Record unit, for the luminous intensity of real time record reactant mixture；
The outfan of described placement module passes sequentially through pressure luminescence unit, in real time detection unit and record unit and then is connected with the input of concentration calculation module.
The beneficial effects of the method for the present invention is: without supporting the use with special reagent, have universality, it is possible to be applied to the fields such as gene test；The pallet of paper chip Electrochemial luminescence detecting instrument is sequentially placed printing three electrode chips and filter paper, and reactant mixture is placed on filter paper, have employed this paper detection cell of filter paper to constitute laboratory on paper, compared with rustless steel detection cell, the consumption of sample and reagent is little, less costly；Have employed laboratory on the paper based on paper detection cell and carry out electrochemiluminescence detection, luminescent substance and interelectrode reaction depend on penetration, compared with Brownian movement, there is higher reaction efficiency, and directly replacing filter paper just can react after the completion of reaction next time, without paper detection cell is carried out, easier.Further, when the luminous intensity of reactant mixture being detected in real time by photomultiplier tube, by the intermediate reaction region printing three electrode chips on pallet is placed in the light-receiving window of photomultiplier tube, takes lucifuge measure and make the mode of photomultiplier tube and the intermediate reaction region accurate contraposition of printing three electrode chips catch faint optical signal, being greatly improved accuracy in detection and detection performance.
Assembly of the invention provides the benefit that: without supporting the use with special reagent, have universality, it is possible to be applied to the fields such as gene test；The pallet of paper chip Electrochemial luminescence detecting instrument is sequentially placed printing three electrode chips and filter paper, and reactant mixture is placed on filter paper, have employed this paper detection cell of filter paper to constitute laboratory on paper, compared with rustless steel detection cell, the consumption of sample and reagent is little, less costly；Have employed laboratory on the paper based on paper detection cell and carry out electrochemiluminescence detection, luminescent substance and interelectrode reaction depend on penetration, compared with Brownian movement, there is higher reaction efficiency, and directly replacing filter paper just can react after the completion of reaction next time, without paper detection cell is carried out, easier.Accompanying drawing explanation
Fig. 1 is the overall flow figure of a kind of paper detection cell electrochemical luminous detection method of the present invention；
Fig. 2 is the structured flowchart of a kind of paper detection cell electrochemical light-emitting detector of the present invention；
Fig. 3 is that the paper detection cell electrochemical luminous detection method adopting the present invention is to detect the standard curve schematic diagram that the serum hepatitis B surface antigen range of linearity is obtained.
Detailed description of the invention
With reference to Fig. 1, a kind of paper detection cell electrochemical luminous detection method, comprise the following steps:
S1, the preparation reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant；
S2, on the pallet of paper chip Electrochemial luminescence detecting instrument, it is sequentially placed printing three electrode chips and filter paper, and reactant mixture is placed on filter paper；
S3, adopt paper chip Electrochemial luminescence detecting instrument photomultiplier tube reactant mixture is carried out electrochemiluminescence detection the electrochemiluminescence intensity of real time record reactant mixture；
S4, according to record electrochemiluminescence intensity adopt standard curve method, calculate the concentration of determinand.
Be further used as preferred embodiment, described step S1 particularly as follows:
Adopt double-antibody method to obtain the reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant, and removed the material failing to combine by magnetic separation method.
Being further used as preferred embodiment, described step S2 includes:
S21, press the button that opens a position of paper chip Electrochemial luminescence detecting instrument, make pallet stretch out from Hatch Opening；
S22, successively placement printing three electrode chips and filter paper on the pallet of paper chip Electrochemial luminescence detecting instrument, and reactant mixture is placed on filter paper；
S23, press storehouse, the pass button of paper chip Electrochemial luminescence detecting instrument, make in tray retracted Hatch Opening and close storehouse.
Being further used as preferred embodiment, described step S3 includes:
S31, the electrode printing three electrode chips is applied voltage, make reactant mixture luminous；
The luminous intensity of reactant mixture is detected in real time by S32, employing cyclic voltammetry by photomultiplier tube；
S33, real time record reactant mixture luminous intensity.
It is further used as preferred embodiment, described step S32, when the luminous intensity of reactant mixture being detected in real time by photomultiplier tube, by being placed in the light-receiving window of photomultiplier tube by the intermediate reaction region printing three electrode chips on pallet, is taked lucifuge measure and makes the mode of photomultiplier tube and the intermediate reaction region accurate contraposition of printing three electrode chips catch faint optical signal.
Wherein, pallet prints the intermediate reaction region of three electrode chips, is the conversion zone of reactant mixture.The intermediate reaction region printing three electrode chips on pallet is placed in the light-receiving window of photomultiplier tube, is to receive all light sent of reactant mixture, to avoid causing part light not collected by photomultiplier tube.
Take lucifuge measure, as long as the light shielding outside, it is to avoid the light sent with reactant mixture is obscured, and interferes.
Make the intermediate reaction region accurate contraposition of photomultiplier tube and printing three electrode chips, be to have correctly received all light sent of reactant mixture, to avoid causing because para-position is inaccurate part light not collected by photomultiplier tube.
Being further used as preferred embodiment, the scanning speed of described cyclic voltammetry is 0.1 volt/second, and the high pressure of described photomultiplier tube is 1000 volts.
Being further used as preferred embodiment, described filter paper is the filter paper of 6 mm dias.
With reference to Fig. 2, a kind of paper detection cell electrochemical light-emitting detector, including with lower module:
Preparation module, for preparing the reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant；
Placement module, for being sequentially placed printing three electrode chips and filter paper on the pallet of paper chip Electrochemial luminescence detecting instrument, and is placed in reactant mixture on filter paper；
Electrochemiluminescence detection and logging modle, for adopting the photomultiplier tube of paper chip Electrochemial luminescence detecting instrument that reactant mixture carries out electrochemiluminescence detection the electrochemiluminescence intensity of real time record reactant mixture；
Concentration calculation module, adopts standard curve method for the electrochemiluminescence intensity according to record, calculates the concentration of determinand；
Described preparation module passes sequentially through placement module and electrochemiluminescence detects with logging modle and then is connected with the input of concentration calculation module.
With reference to Fig. 2, being further used as preferred embodiment, described placement module includes:
Open a position unit, for pressing the button that opens a position of paper chip Electrochemial luminescence detecting instrument, makes pallet stretch out from Hatch Opening；
Placement unit, for placing printing three electrode chips and filter paper successively on the pallet of paper chip Electrochemial luminescence detecting instrument, and is placed in reactant mixture on filter paper；
Pass warehouse receipt unit, for pressing storehouse, the pass button of paper chip Electrochemial luminescence detecting instrument, makes in tray retracted Hatch Opening and closes storehouse；
The outfan of described preparation module pass sequentially through the unit that opens a position, placement unit, pass warehouse receipt unit and then and electrochemiluminescence detection be connected with the input of logging modle.
With reference to Fig. 2, being further used as preferred embodiment, the detection of described electrochemiluminescence includes with logging modle:
Pressure luminescence unit, for the electrode printing three electrode chips is applied voltage, makes reactant mixture luminous；
Detect unit in real time, for adopting cyclic voltammetry by photomultiplier tube, the luminous intensity of reactant mixture to be detected in real time；
Record unit, for the luminous intensity of real time record reactant mixture；
The outfan of described placement module passes sequentially through pressure luminescence unit, in real time detection unit and record unit and then is connected with the input of concentration calculation module.
Below in conjunction with Figure of description and specific embodiment, the present invention is described in further detail.
Embodiment one
The consumption not possessing universality, sample and reagent for prior art is big, relatively costly, reaction rate is low and the problem that needs to clean detection cell after the completion of reaction, the present invention proposes a new technique being realized electrochemiluminescence detection by paper detection cell, and according to electrochemiluminescence Cleaning Principle, design is prepared for being applied to the paper chip Electrochemial luminescence detecting instrument of this new technique, immune detection and gene test for pathogen provide new method, new technique, provide guarantee for researching and developing new pathogen diagnosis reagent.
The paper chip Electrochemial luminescence detecting instrument of the present embodiment mainly includes panel, pallet, photomultiplier tube and control module.Wherein, panel is provided with HT pilot, the button that opens a position, closes storehouse button and Hatch Opening.HT pilot, for indicating by display lamp when photomultiplier tube is high pressure.Hatch Opening, passes in and out for pallet.Photomultiplier tube, for detecting the luminous intensity of reactant mixture.Control module, for the signal that opens a position according to the button that opens a position, close the signal that opens a position of storehouse button and control pallet and calculate the concentration of determinand from Hatch Opening turnover or the electrochemiluminescence intensitometer according to reactant mixture.The present invention first by laboratory applications on paper in scientific instrument, it is proposed that a kind of novel and source property scientific instrument.The paper chip Electrochemial luminescence detecting instrument volume of the present embodiment is little, lightweight, is applicable not only to field quick detection, also provides technical guarantee for the new diagnostic reagent of research.
As it is shown in figure 1, the paper detection cell electrochemical luminous detection method of the present embodiment comprises the following steps:
(1) sandwiching immunity detection method preparation contains the reactant mixture of determinand, electrochemiluminescence active matter and co-reactant routinely.
(2) press the button that opens a position in board, make the pallet that instrument loads sample stretch out Hatch Opening.
(3) on pallet, place the filter paper of printing three electrode chips, diameter 6mm successively, and reactant mixture is placed on filter paper.
(4) press storehouse, the pass button in board, make instrument load in the tray retracted Hatch Opening of sample and close Hatch Opening.
(5) adopt cyclic voltammetry by photomultiplier tube, the luminous intensity of reactant mixture to be detected in real time, and record the luminous intensity of reactant mixture.
(6) by standard curve method, the concentration of determinand is calculated.
Wherein, immunodetection includes but not limited to double-antibody method, direct method and indirect method.
For double-antibody method, the process that realizes of the detection method of the present invention is: first, is obtained charged chemiluminescent labeling and the immune complex of coreaction reagent by double-antibody method, and is removed the material failing to combine by magnetic separation method.Then on electrode, apply certain voltage, make immune complex chemiluminescence.Followed by, adopt the electrochemiluminescence intensity (i.e. the peak area of signal peak) of photomultiplier tube detection reactant mixture, and record the electrochemiluminescence intensity of reactant mixture.Finally, adopt standard curve method, calculate the concentration of determinand.Wherein, what adopt during photomultiplier tube detection luminous intensity is cyclic voltammetry, its sweep speed 0.1V/s, and the high pressure of photomultiplier tube is 1000V.
Fig. 3 then gives the paper detection cell electrochemical luminous detection method adopting the present invention to detect the standard curve schematic diagram that the serum hepatitis B surface antigen range of linearity is obtained.
Compared with prior art, the invention have the advantages that
(1) sandwich assay, direct method, indirect method can be used to carry out immune detection, both the electrochemiluminescence diagnostic kit of domestic and international any producer can have been used, the Novel diagnosis reagent of independent research can be adopted again to detect, without supporting the use with special reagent, there is universality, can be widely applied to the fields such as reducing substances detection, immune detection, gene test, be the couveuse of multiple electrochemiluminescence detection technique.
(2) on the pallet of paper chip Electrochemial luminescence detecting instrument, printing three electrode chips and filter paper it are sequentially placed, and reactant mixture is placed on filter paper, have employed this paper detection cell of filter paper to constitute laboratory on paper, compared with rustless steel detection cell, the consumption of sample and reagent is little, less costly, condition is created, thus providing powerful technical support for creating medical treatment Internet of Things, epidemic monitoring Internet of Things, food safety monitoring Internet of Things, Environmental safety supervision Internet of Things etc. for developing various types of micro-fluidic diagnosing chip.
(3) have employed paper detection cell, compared with rustless steel detection cell, change simpler and convenient.
(4) have employed laboratory on the paper based on paper detection cell and carry out electrochemiluminescence detection, luminescent substance and interelectrode reaction depend on penetration, compared with Brownian movement, there is higher reaction efficiency, and directly replacing filter paper just can react after the completion of reaction next time, without paper detection cell is carried out, easier.
(5) when the luminous intensity of reactant mixture being detected in real time by photomultiplier tube, by the intermediate reaction region printing three electrode chips on pallet is placed in the light-receiving window of photomultiplier tube, takes lucifuge measure and make the mode of photomultiplier tube and the intermediate reaction region accurate contraposition of printing three electrode chips catch faint optical signal, it is greatly improved accuracy in detection and detection performance, it is achieved that caught the optimum performance of faint optical signal by photomultiplier tube.
It is above the preferably enforcement of the present invention has been illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent variations or replacement under the premise without prejudice to spirit of the present invention, and these equivalent deformation or replacement are all contained in the application claim limited range.
Claims (10)
1. a paper detection cell electrochemical luminous detection method, it is characterised in that: comprise the following steps:
S1, the preparation reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant；
S2, on the pallet of paper chip Electrochemial luminescence detecting instrument, it is sequentially placed printing three electrode chips and filter paper, and reactant mixture is placed on filter paper；
S3, adopt paper chip Electrochemial luminescence detecting instrument photomultiplier tube reactant mixture is carried out electrochemiluminescence detection the electrochemiluminescence intensity of real time record reactant mixture；
S4, according to record electrochemiluminescence intensity adopt standard curve method, calculate the concentration of determinand.
2. a kind of paper detection cell electrochemical luminous detection method according to claim 1, it is characterised in that: described step S1 particularly as follows:
Adopt double-antibody method to obtain the reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant, and removed the material failing to combine by magnetic separation method.
3. a kind of paper detection cell electrochemical luminous detection method according to claim 1, it is characterised in that: described step S2 includes:
S21, press the button that opens a position of paper chip Electrochemial luminescence detecting instrument, make pallet stretch out from Hatch Opening；
S22, successively placement printing three electrode chips and filter paper on the pallet of paper chip Electrochemial luminescence detecting instrument, and reactant mixture is placed on filter paper；
S23, press storehouse, the pass button of paper chip Electrochemial luminescence detecting instrument, make in tray retracted Hatch Opening and close storehouse.
4. a kind of paper detection cell electrochemical luminous detection method according to claim 1, it is characterised in that: described step S3 includes:
S31, the electrode printing three electrode chips is applied voltage, make reactant mixture luminous；
The luminous intensity of reactant mixture is detected in real time by S32, employing cyclic voltammetry by photomultiplier tube；
S33, real time record reactant mixture luminous intensity.
5. a kind of paper detection cell electrochemical luminous detection method according to claim 4, it is characterized in that: described step S32, when the luminous intensity of reactant mixture being detected in real time by photomultiplier tube, by being placed in the light-receiving window of photomultiplier tube by the intermediate reaction region printing three electrode chips on pallet, is taked lucifuge measure and makes the mode of photomultiplier tube and the intermediate reaction region accurate contraposition of printing three electrode chips catch faint optical signal.
6. a kind of paper detection cell electrochemical luminous detection method according to claim 4 or 5, it is characterised in that: the scanning speed of described cyclic voltammetry is 0.1 volt/second, and the high pressure of described photomultiplier tube is 1000 volts.
7. a kind of paper detection cell electrochemical luminous detection method according to any one of claim 1-5, it is characterised in that: described filter paper is the filter paper of 6 mm dias.
8. a paper detection cell electrochemical light-emitting detector, it is characterised in that: include with lower module:
Preparation module, for preparing the reactant mixture containing determinand, electrochemiluminescence active matter and co-reactant；
Placement module, for being sequentially placed printing three electrode chips and filter paper on the pallet of paper chip Electrochemial luminescence detecting instrument, and is placed in reactant mixture on filter paper；
Electrochemiluminescence detection and logging modle, for adopting the photomultiplier tube of paper chip Electrochemial luminescence detecting instrument that reactant mixture carries out electrochemiluminescence detection the electrochemiluminescence intensity of real time record reactant mixture；
Concentration calculation module, adopts standard curve method for the electrochemiluminescence intensity according to record, calculates the concentration of determinand；
Described preparation module passes sequentially through placement module and electrochemiluminescence detects with logging modle and then is connected with the input of concentration calculation module.
9. a kind of paper detection cell electrochemical light-emitting detector according to claim 8, it is characterised in that: described placement module includes:
Open a position unit, for pressing the button that opens a position of paper chip Electrochemial luminescence detecting instrument, makes pallet stretch out from Hatch Opening；
Placement unit, for placing printing three electrode chips and filter paper successively on the pallet of paper chip Electrochemial luminescence detecting instrument, and is placed in reactant mixture on filter paper；
Pass warehouse receipt unit, for pressing storehouse, the pass button of paper chip Electrochemial luminescence detecting instrument, makes in tray retracted Hatch Opening and closes storehouse；
The outfan of described preparation module pass sequentially through the unit that opens a position, placement unit, pass warehouse receipt unit and then and electrochemiluminescence detection be connected with the input of logging modle.
10. a kind of paper detection cell electrochemical light-emitting detector according to claim 8, it is characterised in that: the detection of described electrochemiluminescence includes with logging modle:
Pressure luminescence unit, for the electrode printing three electrode chips is applied voltage, makes reactant mixture luminous；
Detect unit in real time, for adopting cyclic voltammetry by photomultiplier tube, the luminous intensity of reactant mixture to be detected in real time；
Record unit, for the luminous intensity of real time record reactant mixture；
The outfan of described placement module passes sequentially through pressure luminescence unit, in real time detection unit and record unit and then is connected with the input of concentration calculation module.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323950A (en) * | 2016-08-10 | 2017-01-11 | 济南大学 | Preparing method for sensor for detecting organophosphorus pesticide residues |
| CN110455886A (en) * | 2019-09-20 | 2019-11-15 | 常熟理工学院 | A kind of photoelectricity COD fast detector |
| CN111198181A (en) * | 2020-01-10 | 2020-05-26 | 苏州易莱生物技术有限公司 | Method and apparatus for electrochemiluminescence detection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070292897A1 (en) * | 2006-06-19 | 2007-12-20 | Hitachi, Ltd. | Analysis kit for living organism and chemical reaction |
| CN204228613U (en) * | 2014-11-13 | 2015-03-25 | 广州军区广州总医院 | A kind of hyperchannel paper electrochemiluminescence immune sensing chip |
| CN104819976A (en) * | 2015-05-15 | 2015-08-05 | 济南大学 | Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection |
-
2016
- 2016-03-04 CN CN201610124405.5A patent/CN105784684B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070292897A1 (en) * | 2006-06-19 | 2007-12-20 | Hitachi, Ltd. | Analysis kit for living organism and chemical reaction |
| CN204228613U (en) * | 2014-11-13 | 2015-03-25 | 广州军区广州总医院 | A kind of hyperchannel paper electrochemiluminescence immune sensing chip |
| CN104819976A (en) * | 2015-05-15 | 2015-08-05 | 济南大学 | Preparation of electrochemical luminescence paper chip and application of chip in hydrogen sulfide detection |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323950A (en) * | 2016-08-10 | 2017-01-11 | 济南大学 | Preparing method for sensor for detecting organophosphorus pesticide residues |
| CN106323950B (en) * | 2016-08-10 | 2018-09-07 | 济南大学 | A kind of preparation method of the sensor of detection organophosphorus pesticide object |
| CN110455886A (en) * | 2019-09-20 | 2019-11-15 | 常熟理工学院 | A kind of photoelectricity COD fast detector |
| CN111198181A (en) * | 2020-01-10 | 2020-05-26 | 苏州易莱生物技术有限公司 | Method and apparatus for electrochemiluminescence detection |
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