WO2018032718A1

WO2018032718A1 - Blood cell and biochemical assay device, and assay method thereof

Info

Publication number: WO2018032718A1
Application number: PCT/CN2017/070574
Authority: WO
Inventors: 徐新; 曹宁; 董自权; 宋成桥; 周强; 郭敏
Original assignee: 江苏英诺华医疗技术有限公司
Priority date: 2016-08-17
Filing date: 2017-01-08
Publication date: 2018-02-22
Also published as: CN106370584A

Abstract

A blood cell and biochemical assay device, and assay method thereof. The assay device comprises: a transfer module, a diluting fluid adding module, a hemolytic agent adding module, a detergent adding module, a first assay container (C1), a second assay container (C2), a cleaning module, a mixing module, and a control module. The first assay container (C1) is used for performing, simultaneously, a first dilution on a blood sample, measurement of indicators such as white blood cell count, hemoglobin, and the like of the blood sample, and another biochemical content assay of the blood sample. The second assay container (C2) is used for performing a second dilution on the blood sample and performing red blood cell and blood platelet assays on the diluted blood sample. The invention realizes rapid completion of a blood routine examination and another biochemical assay, provides improved functionality, and can quickly complete the blood routine examination and the biochemical assay in the event of a clinical emergency. The invention has the advantages of reduced sample waste, ease of operations, and reduced waste generation.

Description

Blood cell and biochemical detector and detection method thereof

Technical field

The invention belongs to the technical field of medical test and detection, in particular to a blood cell and biochemical detector and a detection method thereof.

Background technique

In medical clinical diagnosis, it is often necessary to obtain blood blood cell detection information and biochemical detection information of the patient at the same time. The comprehensive examination information provided by the laboratory can provide more complete guidance information for clinical disease diagnosis and treatment.

The existing blood cell detection and biochemical detection are generally performed separately on the blood analyzer and the biochemical analyzer, so two blood samples and two different instruments must be prepared for the detection to perform two types of detection, which is cumbersome and It is often necessary to manually transfer samples, and the sample coding information is prone to errors in manual transmission. This detection mode cannot meet the needs of rapid detection such as outpatient and emergency. Moreover, the existing instruments for performing routine blood tests and biochemical tests have a single detection project, the structure is complicated, and the process is cumbersome.

Summary of the invention

It is an object of the present invention to provide a blood cell and biochemical detector and a method of detecting the same.

A technical solution for achieving the object of the present invention is: a blood cell and a biochemical detector, the detector comprising a transfer module, a hemolytic agent addition module, a diluent addition module, a cleaning agent addition module, a first detection cup, a second detection cup, and a cleaning Module, mixing module and control module;

The transfer module is configured to transfer a biochemical reagent and a sample, including a biochemical reagent bit, a sample bit, a sample aspirating needle, a first liquid extracting device, and a moving device; the mobile device is used to drive the aspirating needle in the sample position, biochemical Moving between the reagent position, the first detection cup and the second detection cup;

The hemolytic agent addition module includes a hemolytic agent site and a second liquid extraction device for adding a hemolytic agent to the first test cup;

The diluent addition module includes a dilution liquid level and a third liquid extraction device for adding a diluent to the first detection cup and the second detection cup;

The cleaning agent adding module includes a cleaning agent position and a fourth liquid extracting device for adding a cleaning agent to the first detecting cup;

The first detecting cup is used for first dilution of the blood sample, and the first detecting cup wall is provided with a first particle counting detecting device and a transmitted light detecting device, and the first particle counting detecting device is used for the first Check The white blood cell particles in the measuring cup are used for detecting the hemoglobin in the first detecting cup and the biochemical test on the blood sample after adding the biochemical reagent.

The second detecting cup is configured to receive the diluted blood sample transferred from the first detecting cup and perform a second dilution, and the second detecting cup wall is provided with a second particle counting detecting device for the second dilution Red blood cells and platelets in the sample in the back cup are tested;

The cleaning module comprises a sample aspirating washer, a waste liquid discharge pipe and a waste liquid pump, wherein the sample suction needle cleaner is used for cleaning the outer wall of the sample suction needle, and the waste liquid discharge pipe is for discharging the waste liquid in the test cup, and sucking The waste liquid generated after the sample needle cleaner is cleaned, and the waste liquid pump provides power for discharging the waste liquid;

The mixing module agitates and mixes the liquid in the detecting cup by blowing the bottom of the first and second detecting cups;

The control module is configured to control the operation of the transfer module, the diluent addition module, the hemolytic agent addition module, the cleaning agent addition module, the cleaning module, the mixing module, the first detection cup and the second detection cup, and perform detection data Analyze processing, display, print, and output test results.

A method for detecting a blood cell and a biochemical detector includes the following steps:

Step 1. Quantitatively draw the whole blood sample from the sample cup through the aspirating needle, transfer it to the first detection cup, and add the quantitative dilution solution to the first detection cup through the diluent addition module, and pass the mixing module to the first detection. The liquid in the cup is mixed; the outer wall of the suction needle is cleaned by a suction needle cleaner;

Step 2: Aspirating the quantitatively diluted and mixed blood sample from the first test cup by the aspirating needle to the second test cup, and the diluent addition module adds a quantitative dilution solution to the second test cup, and the sample is aspirated The blood sample transferred by the needle is diluted, the liquid in the second test cup is mixed by the mixing module, and the second particle counting detecting device performs the red blood cell and platelet detection on the blood sample mixed in the second detecting cup; through the suction needle cleaner Cleaning the outer wall of the sample needle;

Step 3: adding a quantitative hemolytic agent to the first detection cup through the hemolytic agent adding module, and mixing the liquid in the first detection cup through the mixing module, and the first particle counting detecting device and the transmitted light detecting device respectively perform the first detecting The blood sample after mixing in the cup is subjected to white blood cell detection and hemoglobin detection;

Step 4: Move the aspirating needle to the biochemical reagent position, absorb the quantitative biochemical reagent and transfer it to the first detection cup, mix the liquid in the first detection cup again, and use the transmitted light detecting device to mix and dilute after adding the biochemical reagent. The sample is tested and the test results of the corresponding biochemical items are obtained.

Compared with the prior art and products, the significant effects of the present invention are: (1) the present invention uses a whole blood sample The product quickly completes the routine and biochemical tests of blood on one instrument, and combines the original independent detection processes once, avoiding the repetition of some processes, reducing the sample, saving the detection time and simplifying the operation process; (2) the invention Two different types of blood routine and biochemical tests are performed in one system, and the obtained test results can be very efficiently and accurately unified with the original coded information of the sample; (3) The present invention can be modified by a new design. Better and more accurate to meet the different distribution of different biochemical components in plasma and cells may lead to differences in the detection results of whole blood samples and plasma / serum samples, the accuracy of the test results can meet the needs of clinical testing; (4) the use of the present invention Only one biochemical reagent position and one transmitted light/scattered light detection device can be completed on the basis of a three-class blood cell analyzer. The blood cell and biochemical project tests can be completed at one time. The overall structure is simple, and the detection produces less waste liquid. Conducive to environmental protection.

DRAWINGS

Fig. 1‐1 is a schematic diagram showing the mutual positional relationship between the sample position Cs, the first biochemical reagent position R1, the first detection cup C1 and the second detection cup C2, and a schematic diagram of the movement path of the suction needle.

Fig. 1‐2 is a schematic diagram showing the mutual positional relationship between the sample position Cs, the first biochemical reagent position R1, the second biochemical reagent position R1', the first detection cup C1 and the second detection cup C2, and a schematic diagram of the movement path of the suction needle.

Fig. 1‐3 is a schematic diagram showing the mutual positional relationship between the sample position Cs, the first detection cup C1, the second detection cup C2 and the first biochemical reagent position R1, and a schematic diagram of the movement path of the suction needle.

Fig. 1‐4 is a schematic diagram showing the mutual positional relationship between the sample position Cs, the first detection cup C1, the second detection cup C2, the first biochemical reagent position R1 and the second biochemical reagent position R1', and a schematic diagram of the movement path of the suction needle.

Fig. 1‐5 is a schematic diagram showing the mutual positional relationship between the sample position Cs, the first detection cup C1, the second detection cup C2, the first biochemical reagent position R1 and the second biochemical reagent position R1′, and the arc motion path of the suction needle schematic diagram.

Figure 2‐1 is a schematic view of two test cups of the present invention, in which the first test cup is provided with two light sources and two optical detecting devices.

Figures 2‐2 to 2‐5 show top view of the first test cup when the filters F1, F2 and F3 of three different wavelengths are arranged at different positions.

Figure 3‐1 is a schematic view of two test cups of the present invention, wherein the first test cup is provided with only two light sources and one optical detecting device; and Figures 3‐2 and 3‐3 respectively set three filters in the first A top view of the test cup at different positions.

Figure 4 is a schematic view showing the structure of the biochemical detector of the present invention.

Figure 5 is a schematic view of the syringe cleaner of the present invention.

Fig. 6 is a graph showing the correlation analysis between the existing biochemical analyzer and the analyzer of the present invention for detecting CRP results.

Fig. 7 is a graph showing the correlation analysis between the existing biochemical analyzer and the analyzer of the present invention for detecting HbA1c results.

detailed description

A blood cell and biochemical detector and detection method thereof, comprising a transfer module, a hemolytic agent addition module, a diluent addition module, a cleaning agent addition module, a first detection cup, a second detection cup, a cleaning module, a mixing module And control modules.

The transfer module is configured to transfer a reagent and a sample, including a biochemical reagent bit, a sample bit, a sample aspirating needle, a first liquid extracting device, a connecting line, and a moving device; the moving device is configured to drive the aspirating sample in the sample Position, biochemical reagent position, movement between the first detection cup and the second detection cup;

The hemolytic agent addition module includes a hemolytic agent site and a second liquid extraction device for adding a hemolytic agent to the first test cup; the diluent addition module includes a dilution liquid level and a third liquid extraction device for Adding a diluent to the first detection cup and the second detection cup; the cleaning agent adding module includes a cleaning agent position and a fourth liquid extraction device for adding a cleaning agent to the first detection cup;

The first detecting cup is used for first dilution of the blood sample, and the first detecting cup wall is provided with a first particle counting detecting device and a transmitted light detecting device, and the first particle counting detecting device is used for the white blood cell particles in the cup Performing detection, the transmitted light detecting device is used for detecting hemoglobin and performing biochemical test on the blood sample after adding the biochemical reagent;

The second detecting cup is configured to receive the diluted blood sample transferred from the first detecting cup, and perform a second dilution on the blood sample, and the second detecting cup wall is provided with a second particle counting detecting device for the second Red blood cells and platelets in the cup after the second dilution;

The mixing module realizes mixing the liquid in the detecting cup by agitating the bottom of the first and second detecting cups to agitate the liquid in the detecting cup;

The cleaning module comprises a suction needle cleaner, a waste liquid discharge tube and a waste liquid pump, and the sample suction needle cleaner is used for cleaning the outer wall of the suction needle to reduce cross-contamination, and the waste liquid is discharged as shown in FIG. 5 . The tube is used for removing the waste liquid in the test cup and the waste liquid generated after the sample needle cleaner is cleaned, and the waste liquid pump provides power for discharging the waste liquid;

The control module is configured to control a transfer module, a diluent addition module, a hemolytic agent addition module, and a cleaning The detection device of the agent addition module, the cleaning module, the mixing module, the first detection cup and the second detection cup work, and performs detection data analysis processing, display, printing, and output detection results.

Further, the first detection cup wall is further provided with a scattered light detecting device for performing biochemical detection on the blood sample after adding the biochemical reagent.

Further, the scattered light detecting device and the transmitted light detecting device are single wavelength detecting devices or multi-wavelength detecting devices. The scattered light detecting means and the transmitted light detecting means switch the wavelength according to the detection, and the switching of the detected wavelength is realized by switching the light source, switching the voltage, or installing a monochromator device capable of changing the wavelength before the detector.

Further, the transfer module includes more than one biochemical reagent position.

Further, the biochemical detector is further provided with a washer for cleaning the outer wall of the sample needle.

Further, a shielding cover is disposed around the first detecting cup and the second detecting cup, and an opening is arranged above the shielding cover, and a cover that can be automatically opened and closed is provided at the opening, and the cover is closed when detecting, and the cover is added when the sample is loaded. Open.

Further, the first test cup also has a constant temperature and heating device to ensure accurate biochemical test results.

The invention also provides a method for detecting a blood cell and a biochemical detector, comprising the following steps:

Step 4: Move the aspirating needle to the biochemical reagent position, absorb the quantitative biochemical reagent and transfer it to the first detection cup, mix the liquid in the first detection cup again, and mix the biochemical reagent by using the transmitted light detecting device. The diluted sample is tested and the test results of the corresponding biochemical items are obtained.

Further, in step 4, the scattered light detecting device is used to replace the transmitted light detecting device to detect the biochemical component in the sample, and the corresponding biochemical item detecting result is obtained.

Further, after the first particle counting detecting device and the transmitted light detecting device respectively perform the detection of white blood cells and hemoglobin, the glycated hemoglobin reagent is added to the first detecting cup and mixed, and the scattered light detecting device performs the glycated hemoglobin detection on the mixed liquid. The ratio of glycated hemoglobin to hemoglobin was calculated based on the hemoglobin test result and the glycated hemoglobin test result.

For the detection results of biochemical components mainly distributed in serum and plasma, since the results obtained by using whole blood test are significantly different from those of using serum or plasma samples, the instrument automatically performs the biochemical test results of this part of the project. Amend to ensure that the results of biochemical tests detected by this instrument are consistent with the results of serum and plasma tests. The specific correction method is:

The test result is the concentration of a certain biochemical component per unit volume of plasma/serum, wherein serum/plasma volume = whole blood volume - blood cell volume, wherein "/" means or; cell volume = total platelet count x whole platelet volume + Number of red blood cells in whole blood × average volume of red blood cells + number of white blood cells in whole blood × total white blood cell volume × correction factor b, correction factors a and b range from 0.3 to 4.0, respectively.

The volume of white blood cells can also be calculated according to the following formula:

White blood cell volume = average white blood cell volume × large white blood cell number × correction factor c + small white blood cell volume × small white blood cell number × correction factor d, the correction factors c and d range from 0.3 to 4.0, respectively;

Or the volume of white blood cells can also be calculated according to the following formula:

Leukocyte volume = average lymphocyte volume × number of lymphocytes × correction factor e + neutrophil volume × number of neutrophils × correction factor f + intermediate cell volume × number of intermediate cells × correction factor g, correction factors e, f and g The range is 0.3‐‐4.0.

However, if the test result is required to be a certain biochemical component in whole blood, rather than a certain biochemical component in serum or plasma, the above correction is not required.

Combined with Figure 1‐1~1‐5, the sample position, the first test cup, the second test cup, the first biochemical reagent position and the second biochemical reagent position of the detector are designed in the movement track of the suction needle. The specific layout is as follows: :

As shown in Figure 1-1: sample position Cs → first biochemical reagent position R1 → first detection cup C1 → second detection Cup C2;

As shown in Figure 1-2: sample position Cs → first biochemical reagent position R1 → second biochemical reagent position R1 ' → first detection cup C1 → second detection cup C2;

As shown in Figure 1-3: sample position Cs → first test cup C1 → second test cup C2 → first biochemical reagent position R1;

As shown in Figure 1-4 and 1-5: sample position Cs → first test cup C1 → second test cup C2 → first biochemical reagent position R1 → second biochemical reagent position R1';

The invention will now be further described with reference to the accompanying drawings and embodiments.

Example 1

In combination with FIG. 1 -1, FIG. 2 -1 and FIG. 4, blood routine and single-reagent biochemical items are detected in this embodiment.

The detector comprises a transfer module, a hemolytic agent addition module, a diluent addition module, a cleaning agent addition module, a first detection cup, a second detection cup, a cleaning module, a mixing module and a control module;

During the detection, the suction needle N1 moves into the sample cup, the second valve V2 is closed, the first valve V1 is opened, the first liquid extraction device S1 is pulled back, the quantitative sample is sucked into the suction needle N1, and the mobile device drives the suction needle. N1 moves to the first detecting cup C1, the first valve V1 is closed, the second valve V2 is opened, and the first liquid extracting device S1 pushes the blood sample of the sucking needle N1 into the first detecting cup C1; the diluent adding module The sixth valve V6 is opened, the second valve V2, the thirteenth valve V13 and the fifth valve V5 are closed, and the third liquid extracting device S3 is pulled back to draw the diluent R3 into the third liquid extracting device S3, and the sixth valve V6 is closed. The thirteenth valve V13 is opened, and the third liquid extracting device S3 is pushed by the piston, and the diluent in the original third liquid extracting device S3 enters the first detecting cup C1 along N3; the tenth valve V10 and the tenth in the mixing module The second valve V12 is closed, the ninth valve V9 and the eleventh valve V11 are opened, the air pump P1 is blown, and the driving gas enters the bottom of the first detecting cup C1 along the pipeline to mix the liquid in the cup.

The aspirating needle N1 sucks a partially diluted diluted sample from the first detecting cup C1, and transfers to the second detecting cup C2, and the sixth valve V6 in the diluent adding module is opened, the fifth valve V5, the second valve V2 and the first The thirteenth valve V13 is closed, the third liquid extracting device S3 pulls back the suction to draw the quantitative dilution liquid R3, the sixth valve V6 and the thirteenth valve V13 are closed, and the fifth valve V5, the first valve V1 and the second valve V2 are opened, The third liquid extracting device S3 pushes the quantitative diluent in the third liquid extracting device S3 into the second detecting cup C2 through the second diluent needle N3', and enters the sucking needle N1 through the pipe, and the inside of the sucking needle N1 Wash it. The ninth valve V9 and the twelfth valve V12 in the mixing module are closed, and the tenth valve V10 and the eleventh valve V11 are opened, the air pump P1 is blown, and the driving gas enters the bottom of the second detecting cup C2 along the pipeline to mix the liquid in the cup. The second particle count detecting means D5 detects the number and volume of sample platelets and red blood cells in the second detecting cup C2.

The external needle cleaner cleans the outside of the suction needle N1. After the fourth valve V4 is opened, the third valve V3 is closed, the second liquid extracting device S2 is pulled back, the hemolytic agent R2 is sucked into the second liquid extracting device S2, the fourth valve V4 is closed, the third valve V3 is opened, and the second liquid is drawn. The device S2 is advanced by the piston to cause the quantitative hemolytic agent to enter the first test cup C1. In the mixing module, the eleventh valve V11 and the ninth valve V9 are opened, the tenth valve V10 and the twelfth valve V12 are closed, the air pump P1 is blown, and the driving gas enters the bottom of the first detecting cup C1 along the pipeline, and the mixing cup is mixed. Medium liquid. The first particle counting device D4 in the first detecting cup C1 detects the number and volume of white blood cells in the cup, and the first transmitted light source L1 or the second transmitted light source L1' is turned on, the first transmitted light detecting device D1 or the second transmitted light detecting device D1' detects hemoglobin content.

The aspirating needle N1 moves to the biochemical reagent position R1, and the aspirating needle N1 penetrates into the biochemical reagent position R1, and is sucked by the biochemical reagent position R1 under the cooperation of the first liquid extracting device S1, the first valve V1 and the second valve V2. Quantitative biochemical reagent, then the sample needle N1 moves to the first test cup C1, the biochemical reagent is added to the first test cup C1, the tenth valve V10 and the twelfth valve V12 of the mixing module are closed, the ninth valve V9 and the The eleventh valve V11 is opened, the air pump P1 is blown, and the driving gas enters the bottom of the first detecting cup C1, and the liquid in the cup is mixed. As shown in FIGS. 2-2, 2-3, 2-4, and 2-5, the first transmitted light source L1 is turned on, and the first transmitted light detecting device D1 detects the transmitted light signal to obtain a single reagent biochemical item detection result.

After the test is finished, the ninth valve V9, the tenth valve V10, the twelfth valve V12, the waste liquid pump P2 are pumped, and the detection waste liquid in each test cup, the needle outer cleaner and the pipeline is discharged into the waste liquid collector. After the eighth valve V8 in the cleaning agent addition module is opened, the seventh valve V7 is closed, the fourth liquid extraction device S4 is pulled back, the cleaning agent R4 is sucked into the fourth liquid extraction device S4, and then the eighth valve V8 is closed, and the seventh When the valve V7 is opened, the fourth liquid extracting device S4 is advanced, and the cleaning agent R4 is injected into the first detecting cup C1, and then the ninth valve V9 and the twelfth valve V12 are opened, the waste liquid pump P2 is evacuated, and the cleaning waste liquid is discharged into the waste. Liquid collector. The sixth valve V6 in the diluent addition module is opened, the fifth valve V5, the second valve V2 and the thirteenth valve V13 are closed, the third liquid extraction device S3 piston pulls back to draw the quantitative dilution liquid R3, and then the sixth valve V6 Closing, the fifth valve V5, the thirteenth valve V13, the first valve V1 and the second valve V2 are opened, and the third liquid extracting device S3 pushes the piston to pass the quantitative diluent in the third liquid extracting device S3 through the first diluent needle N3 and the second diluent needle N3' are injected into the first detecting cup C1 and the second detecting cup C2, At the same time, the suction needle N1 is entered through the pipeline, and the inside of each test cup and the suction needle N1 is cleaned. The suction needle N1 also performs external cleaning of the needle at this time. After the cleaning is completed, the ninth valve V9, the tenth valve V10, the twelfth valve V12, the waste liquid pump P2 are pumped, and the cleaning waste liquid is discharged into the waste liquid collector.

In Fig. 4, the suction needle N1, the first liquid extraction device S1, the first valve V1 and the second valve V2 constitute a transfer module; the hemolytic agent needle N2, the second liquid extraction device S2, the third valve V3 and the fourth valve V4 constitute Hemolysis agent addition module; diluent needles N3' and N3, third liquid extraction device S3, fifth valve V5, sixth valve V6, thirteenth valve V13 constitute a diluent addition module; cleaning agent needle N4, fourth liquid extraction The device S4, the seventh valve V7 and the eighth valve V8 constitute a cleaning agent adding module; the air pump P1, the ninth valve V9, the tenth valve V10, the eleventh valve V11 and the twelfth valve V12 constitute a mixing module.

Example 2

Referring to FIG. 1 - 2, FIG. 2 - 1 and FIG. 4, the biochemical detector of the present embodiment is different from the embodiment 1 in that the blood routine and the two-reagent biochemical project are detected in the embodiment, that is, the embodiment includes two biochemicals. Reagent position.

The suction needle N1 moves to the first biochemical reagent position R1, and the suction needle N1 penetrates into the first biochemical reagent position R1, and is matched by the first liquid extraction device S1, the first valve V1 and the second valve V2, by the first The first biochemical reagent is taken up in the biochemical reagent position R1, and then the aspirating needle N1 is moved to the first detecting cup C1, the first biochemical reagent is added to the first detecting cup C1, and the tenth valve V10 and the tenth in the mixing module are The second valve V12 is closed, the ninth valve V9 and the eleventh valve V11 are opened, the air pump P1 is blown, and the driving gas enters the bottom of the first detecting cup C1 to mix the liquid in the cup. Then, the sample needle N1 is moved to the second biochemical reagent position R1', and the second biochemical reagent is aspirated in the same manner and transferred to the first test cup C1 to turn on the first transmitted light source L1, and the first transmitted light detecting device D1 detects The light signal is transmitted to obtain the detection result of the two-reagent biochemical project.

Example 3

The biochemical detector of the present embodiment is different from the embodiment 1 in that the first detecting cup of the present embodiment is provided with a first transmitted light source L1 and a scattered light source L2, and the first transmitted light source L1 and the scattering are combined with FIG. The light source L2 shares a detection device D3. The first transmitted light source L1 is disposed opposite the detecting device D3, and the scattered light source L2 and the detecting device D3 are at an angle of 30-120.

The first transmitted light source L1 is turned on, the scattered light source L2 is turned off, and the detecting device D3 detects the transmitted light signal to obtain a biochemical item detection result.

Example 4

The difference between this embodiment and the third embodiment is that the scattered light source L2 is turned on, the first transmitted light source L1 is turned off, and the detecting device D3 detects the scattered light signal to obtain the biochemical item detection result.

Example 5

The biochemical detector of the present embodiment is different from

Embodiments

1 and 2 in that the present embodiment obtains the biochemical item detection result by the second transmission light source L1' and the second transmitted light detecting device D1'.

Example 6

2 to 2 to 2 to 5 and FIGS. 3 to 2 to 3 to 3, the present embodiment detects by using the first filter F1, the second filter F2, and the third filter F3 of different wavelengths. Select a specific wavelength based on the detection needs.

Taking the dual-wavelength method for measuring glycated hemoglobin as an example, the first filter F1 is rotated to the light-emitting portion of the light source and before the receiving portion of the detecting device, the dominant wavelength is set to 510 nm, the absorbance of the detected sample is A1; and the second filter F2 is rotated to the light source. In the middle of the detection device, the sub-wavelength is set to 600 nm, the absorbance of the sample is measured as A2, and the sample absorbance A _sample = A1‐A2. By using the dual-wave method, the influence of the interfering substances in the sample on the detection result can be effectively eliminated.

Example 7

In this embodiment, 20 different samples are detected by using the existing biochemical analyzer and the analyzer of the present invention, and the correlation of the results is compared. The statistical data is shown in Table 1.

Table 1 Comparison of the results of two biochemical components detected by different biochemical analyzers and analyzers of the present invention

20 different samples were detected by the existing biochemical analyzer and the analyzer of the present invention, and the correlation analysis was performed, as shown in FIG. 6 and FIG. 7, FIG. 6 is the CRP detection result, and FIG. 7 is the HbA1c detection result, two types. Correlation of test results of different methods: R _CRP =0.9974≥0.95, R _HbA1 c=0.9783≥0.95, indicating that the correlation between the existing biochemical analyzer and the analyzer of the present invention is good.

Claims

A blood cell and biochemical detector, characterized in that the detector comprises a transfer module, a hemolytic agent addition module, a diluent addition module, a cleaning agent addition module, a first detection cup, a second detection cup, a cleaning module, a mixing module And control module;

The transfer module is configured to transfer a biochemical reagent and a sample, including a biochemical reagent bit, a sample bit, a sample aspirating needle, a first liquid extracting device, and a moving device; the mobile device is used to drive the aspirating needle in the sample position, biochemical Moving between the reagent position, the first detection cup and the second detection cup;

The hemolytic agent addition module includes a hemolytic agent site and a second liquid extraction device for adding a hemolytic agent to the first test cup;

The diluent addition module includes a dilution liquid level and a third liquid extraction device for adding a diluent to the first detection cup and the second detection cup;

The cleaning agent adding module includes a cleaning agent position and a fourth liquid extracting device for adding a cleaning agent to the first detecting cup;

The first detecting cup is used for first dilution of the blood sample, and the first detecting cup wall is provided with a first particle counting detecting device and a transmitted light detecting device, and the first particle counting detecting device is used for the first The detection of white blood cell particles in the test cup is used for detecting hemoglobin in the first test cup and performing biochemical test on the blood sample after adding the biochemical reagent.

The second detecting cup is configured to receive the diluted blood sample transferred from the first detecting cup and perform a second dilution, and the second detecting cup wall is provided with a second particle counting detecting device for the second dilution Red blood cells and platelets in the sample in the back cup are tested;

The cleaning module comprises a sample aspirating washer, a waste liquid discharge pipe and a waste liquid pump, wherein the sample suction needle cleaner is used for cleaning the outer wall of the sample suction needle, and the waste liquid discharge pipe is used for discharging the waste liquid and the sample sample in the test cup. The waste liquid generated after the needle cleaner is cleaned, and the waste liquid pump provides power for discharging the waste liquid;

The mixing module agitates and mixes the liquid in the detecting cup by blowing the bottom of the first and second detecting cups;

The control module is configured to control the operation of the transfer module, the diluent addition module, the hemolytic agent addition module, the cleaning agent addition module, the cleaning module, the mixing module, the first detection cup and the second detection cup, and perform detection data Analyze processing, display, print, and output test results.
The blood cell and biochemical detector according to claim 1, wherein a scattering light detecting device is further disposed on the wall of the first detecting cup for performing biochemical inspection on the blood sample after adding the biochemical reagent Measurement.
The blood cell and biochemical detector according to claim 2, wherein said scattered light detecting means and transmitted light detecting means are multi-wavelength detecting means.
The blood cell and biochemical detector according to claim 1, wherein the biochemical detector further comprises a washer for cleaning the outer wall of the sample needle.
The blood cell and biochemical detector of claim 1 wherein said transfer module comprises more than one biochemical reagent site.
The blood cell and biochemical detector according to claim 1, wherein the first detecting cup is provided with a constant temperature and heating means on the outer circumference.
A method for detecting a blood cell and a biochemical detector according to claim 1, wherein the method comprises the following steps:

Step 1. Quantitatively draw the whole blood sample from the sample cup through the aspirating needle, transfer it to the first detection cup, and add the quantitative dilution solution to the first detection cup through the diluent addition module, and pass the mixing module to the first detection. The liquid in the cup is mixed; the outer wall of the suction needle is cleaned by a suction needle cleaner;

Step 2: Aspirating the quantitatively diluted and mixed blood sample from the first test cup by the aspirating needle to the second test cup, and the diluent addition module adds a quantitative dilution solution to the second test cup, and the sample is aspirated The blood sample transferred by the needle is diluted, the liquid in the second test cup is mixed by the mixing module, and the second particle counting detecting device performs the red blood cell and platelet detection on the blood sample mixed in the second detecting cup; through the suction needle cleaner Cleaning the outer wall of the sample needle;

Step 3: adding a quantitative hemolytic agent to the first detection cup through the hemolytic agent adding module, and mixing the liquid in the first detection cup through the mixing module, and the first particle counting detecting device and the transmitted light detecting device respectively perform the first detecting The blood sample after mixing in the cup is subjected to white blood cell detection and hemoglobin detection;

Step 4: Move the aspirating needle to the biochemical reagent position, absorb the quantitative biochemical reagent and transfer it to the first detection cup, mix the liquid in the first detection cup again, and use the transmitted light detecting device to mix and dilute after adding the biochemical reagent. The sample is tested and the test results of the corresponding biochemical items are obtained.
The method for detecting a blood cell and a biochemical detector according to claim 7, wherein in step 4, the scattered light detecting means is used to replace the transmitted light detecting means to detect the biochemical component in the sample, and the corresponding biochemical item detecting result is obtained. .
The method for detecting a blood cell and a biochemical detector according to claim 7, wherein When the instrument is tested, the biochemical test results are automatically corrected. The test result correction formula is as follows:

The correction factor a is used to correct the error caused by other influencing factors and its range is 0.3‐4.0.
The method for detecting a blood cell and a biochemical detector according to claim 7, wherein the first particle counting detecting device and the transmitted light detecting device respectively perform the detection of white blood cells and hemoglobin, and then add the glycated hemoglobin reagent to the first detecting cup. The mixed light and scattered light detecting device performs glycated hemoglobin detection on the mixed liquid, and calculates the ratio of glycated hemoglobin to hemoglobin based on the hemoglobin test result and the glycated hemoglobin test result.