CN106124751A - Blood cell and analysis of biochemical instrument and method - Google Patents
Blood cell and analysis of biochemical instrument and method Download PDFInfo
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- CN106124751A CN106124751A CN201610681443.0A CN201610681443A CN106124751A CN 106124751 A CN106124751 A CN 106124751A CN 201610681443 A CN201610681443 A CN 201610681443A CN 106124751 A CN106124751 A CN 106124751A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Abstract
The invention discloses a kind of blood cell and analysis of biochemical instrument and method, this analyser includes that shift module, the first hemolytic agent add module, the second hemolytic agent adds module, the 3rd hemolytic agent adds module, diluent interpolation module, abluent add module, cleaning module, mixing module, control module, the first detection cup, the second detection cup, the 3rd detection cup and the 4th detect cup.Present invention can be implemented in and be rapidly completed blood cell routine on same analyser and plant the detection of above biochemical project so that the detection of instrument is with better function, sample consumption less, detection efficiency is higher, for clinical disease diagnosis offer more comprehensively information.
Description
Technical field
The invention belongs to medical test detection technique field, particularly a kind of blood cell and analysis of biochemical instrument and side
Method.
Background technology
Often need laboratory to obtain blood samples of patients hemocyte detection information and biochemistry detection in medical clinic applications simultaneously
Information, the Integrated Checkout information that laboratory provides can provide more perfect tutorial message to clinical disease diagnosis and treatment.
The detection of existing blood cell and biochemistry detection are usually and carry out on blood analyser and biochemistry analyzer respectively, because of
Two parts of blood samples and two different instruments must be prepared during this detection, operate relatively complicated, and sample encoded information is in manual delivery
In easily make a mistake, this detection pattern is not particularly suitable for the needs of the quickly detection such as outpatient service, emergency treatment.And existing perform blood
Conventional and biochemistry detection the instrument detection project of liquid is single, and structure is more complicated, and flow process is cumbersome.
Summary of the invention
The purpose of invention is to provide a kind of blood cell and analysis of biochemical instrument and method.
The technical scheme realizing the object of the invention is: a kind of blood cell and analysis of biochemical instrument, including shift module,
First hemolytic agent add module, second hemolytic agent add module, diluent add module, abluent add module, cleaning module,
Mixing module, control module, the first detection cup, the second detection cup and the 3rd detection cup；
Described shift module is used for shifting biochemical reagents and sample, including biochemical reagents position, sample position, suction needle, first
Liquid extracting device, connecting line and mobile device；Described mobile device is used for driving suction needle at sample position, biochemical reagents
Move between position, the first detection cup, the second detection cup and the 3rd detection cup；
Described first hemolytic agent adds module and includes the first hemolytic agent position and second liquid draw-out device, for the first inspection
Survey in cup and add the first hemolytic agent；
Described second hemolytic agent adds module and includes the second hemolytic agent position and the 5th liquid extracting device, for the second inspection
Survey in cup and add the second hemolytic agent；
Described diluent adds module and includes a dilution liquid level and the 3rd liquid extracting device, for the first detection
Cup, the second detection cup, the 3rd detection cup, suction needle and suction needle washer inject diluent；
Described abluent adds module and includes an abluent position and the 4th liquid extracting device, for the first detection cup
Abluent is added with in the second detection cup；
Described cleaning module includes suction needle washer, connecting line, waste liquid discharge pipe and waste drains pump, described suction needle
Washer is used for cleaning suction needle outer wall, and it is clear that described waste liquid discharge pipe is used for discharging waste liquid and suction needle washer in detection cup
The waste liquid produced after washing, described waste drains pump is discharged for waste liquid and is provided power；
Described mixing module is by air-blowing bottom first, second, and third detection cup, to the aqueous agitation in detection cup
Mixing；
It is respectively arranged with on the wall of cup of the first detection cup, the second detection cup and the 3rd detection cup and is provided with the first grain count
Detection device, the second grain count detection device and the 3rd grain count detection device, the first grain count detection device and the
Two grain count detection devices for carrying out nucleated cell detection to liquid in cup, and the 3rd grain count detection device is for red
Cell and hematoblastic quantity and volume detect；One of them detection cup of first detection cup and the second detection cup is arranged
Having transmission optical detection device, detect for hemoglobin, in the first detection cup and the second detection cup, at least one detection cup is arranged
There is biochemical detection device, after being used for adding biochemical reagents, carry out biochemical project detection；
Described control module in order to control shift module, diluent adds module, the first hemolytic agent adds module, second molten
Module, abluent interpolation module, cleaning module, mixing module, the first detection cup, the second detection cup and the 3rd detection are added in blood agent
Cup work, and perform to detect Data Analysis Services, show, print, output detections result and and external communication.
The present invention also provides for a kind of blood cell and analysis of biochemical instrument, adds including shift module, the first hemolytic agent
Module, the second hemolytic agent add module, the 3rd hemolytic agent adds module, diluent adds module, abluent adds module, cleaning
Module, mixing module, control module, the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup；
Described shift module is used for shifting biochemical reagents and sample, including biochemical reagents position, sample position, suction needle, first
Liquid extracting device, connecting line and mobile device；Described mobile device is used for driving suction needle at sample position, biochemical reagents
Move between position, the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup；
Described first hemolytic agent adds module and includes the first hemolytic agent position and second liquid draw-out device, for the first inspection
Survey in cup and add the first hemolytic agent；
Described second hemolytic agent adds module and includes the second hemolytic agent position and the 5th liquid extracting device, for the second inspection
Survey in cup and add the second hemolytic agent；
Described 3rd hemolytic agent adds module and includes the 3rd hemolytic agent position and the 6th liquid extracting device, for the 4th inspection
Survey and cup adds the 3rd hemolytic agent；
Described diluent adds module and includes a dilution liquid level and the 3rd liquid extracting device, for the first detection
Cup, the second detection cup, the 3rd detection cup, the 4th detection cup, suction needle and suction needle washer inject diluent；
Described abluent adds module and includes an abluent position and the 4th liquid extracting device, for the first detection
Cup, the second detection cup and the 4th detection cup add abluent；
Described cleaning module includes suction needle washer, connecting line, waste liquid discharge pipe and waste drains pump, described suction needle
Washer is used for cleaning suction needle outer wall, and it is clear that described waste liquid discharge pipe is used for discharging waste liquid and suction needle washer in detection cup
The waste liquid produced after washing, described waste drains pump is discharged for waste liquid and is provided power；
Described mixing module is by air-blowing bottom first, second, third and fourth detection cup, to the liquid in detection cup
Agitation mixing；
It is respectively arranged with on the wall of cup of the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup and is provided with
First grain count detection device, the second grain count detection device, the 3rd grain count detection device and the 4th grain count
Detection device, the first grain count detection device, the second grain count detection device and the 4th grain count detection device are used for
Liquid in cup carries out nucleated cell detection, and the 3rd grain count detection device is for erythrocyte and hematoblastic quantity and body
Amass and detect；One of them detection cup of first detection cup, the second detection cup and the 4th detection cup is provided with the inspection of transmission light
Surveying device, detect for hemoglobin, in the first detection cup, the second detection cup and the 4th detection cup, at least one detection cup is arranged
There is biochemical detection device, after being used for adding biochemical reagents, carry out biochemical project detection；
Described control module in order to control shift module, diluent adds module, the first hemolytic agent adds module, second molten
Blood agent add module, the 3rd hemolytic agent add module, abluent add module, cleaning module, mixing module, first detection cup,
Second detection cup, the 3rd detection cup and the 4th detection cup work, and perform to detect Data Analysis Services, show, print, export inspection
Survey result and and external communication.
The present invention also provides for a kind of analysis method of blood cell and analysis of biochemical instrument, and the method includes following step
Rapid:
Step 1, in specimen cup, quantitatively draw whole blood sample by suction needle, quantitatively transfer to the first detection cup, second
Detection cup and the 4th detects in cup, and diluent adds module difference in the first detection cup, the second detection cup and the 4th detection cup
Add quantitative diluent, and by mixing module mixing the first detection cup, the second detection cup and the 4th detection cup；By inhaling sample
Suction needle outer wall is carried out by pin washer；
Step 2, in the first, second, the 4th detection any one cup of cup, draw quantitative dilution, mixing by suction needle after
Blood sample be transferred to the 3rd detection cup, mix with the diluent in cup, mixing module detects liquid blending in cup to the 3rd, the
Three grain count detection devices carry out erythrocyte and platelets analysis to blood sample after mixing in the 3rd detection cup；Clear by suction needle
Wash device suction needle outer wall is carried out；
Step 3, module of being added by the first hemolytic agent add the first quantitative hemolytic agent and through mixed in the first detection cup
Even module is to liquid blending in the first detection cup, and the first grain count detection device and transmission optical detection device are respectively to the first inspection
After surveying cup mixing, the nucleated cell in blood sample and hemoglobin detect；
Step 4, by the second hemolytic agent adding set to second detection cup in add the second hemolytic agent, the second grain count
Nucleated cell is detected by detection device；
Step 5, by the 3rd hemolytic agent adding set to the 4th detection cup in add the 3rd hemolytic agent, the 4th grain count
Nucleated cell is detected by detection device；
Suction needle after step 6, cleaning moves to biochemical reagents position, draws the first quantitative biochemical reagents and is transferred to
First detection cup, liquid in mixing the first detection cup, is mixed after adding biochemical reagents by the first biochemical detection device again
Dilute sample detect, it is thus achieved that the testing result of corresponding biochemical project；
Suction needle after step 7, cleaning moves to the second biochemical reagents position again, draws quantitative the second biochemical reagents and adds
To the second detection cup, perform the second biochemical project by the second biochemical detection device and detect；Suction needle after cleaning is quantitatively inhaled
Take the third biochemical reagents, add the 4th detection cup, perform the third biochemical project by the 3rd biochemical detection device and detect.
Compared with prior art and product, the remarkable result of the present invention is: (1) present invention uses a whole blood sample one
Blood routine and multinomial biochemistry detection it is rapidly completed, by effective for the part flow process of the most independent detection process on platform instrument
Integrate, it is to avoid the repetition of part flow process, enormously simplify operation sequence, save the detection time；(2) present invention simply uses
Whole blood just can complete biochemical project detection, and eliminates whole blood sample detection biochemical component by the modification method of the present invention
Difference with the biochemistry detection result of serum/plasma sample, it is ensured that testing result is accurately and can be with the biochemistry detection side of classics
Method compares；(3) the analyser population structure that the present invention uses is simple, only one suction needle just can complete whole sample and
The transfer of biochemical reagents or distribution；(4) sample required for the detection of this instrument is less, cell detection and biochemical inspection during detection
Surveying and share sample, dilution and cleaning reagent, the waste liquid therefore produced is less, more environmentally friendly.
Accompanying drawing explanation
Fig. 1-1 is sample position Cs, the first biochemical reagents position R1, first detection cup C1, second detection cup C2 and the 3rd detection
Mutual alignment relation schematic diagram between cup C3 and suction needle movement locus schematic diagram.
Fig. 1-2 is sample position Cs, the first biochemical reagents position R1, the second biochemical reagents position R1 ', first detection cup C1, second
Mutual alignment relation schematic diagram and suction needle movement locus between detection cup C2, the 4th detection cup C4 and the 3rd detection cup C3 show
It is intended to.
Fig. 1-3 is sample position Cs, the first biochemical reagents position R1, the second biochemical reagents position R1 ', the 3rd biochemical reagents position R1 ",
Mutual alignment relation schematic diagram between first detection cup C1, the second detection cup C2, the 4th detection cup C4 and the 3rd detection cup C3
And suction needle movement locus schematic diagram.
Fig. 1-4 be sample position Cs, first detection cup C1, second detection cup C2, the 4th detection cup C4, the 3rd detection cup C3,
First biochemical reagents position R1, the second biochemical reagents position R1 ' and the 3rd biochemical reagents position R1 " between mutual alignment relation schematic diagram
And suction needle movement locus schematic diagram.
Fig. 1-5 be sample position Cs, first detection cup C1, second detection cup C2, the 4th detection cup C4, the 3rd detection cup C3,
First biochemical reagents position R1, the second biochemical reagents position R1 ' and the 3rd biochemical reagents position R1 " between mutual alignment relation schematic diagram
And suction needle movement in a curve track schematic diagram.
Fig. 2 is that the present invention four detects cup schematic diagram.
Fig. 3-1～Fig. 3-3 detects cup top view for the present invention first.
Fig. 4 is analyser structural representation of the present invention.
Fig. 5 is that existing biochemical instruments detects CRP results relevance analysis chart with analyser of the present invention.
Fig. 6 is that existing biochemical instruments detects HbA1c results relevance analysis chart with analyser of the present invention.
Detailed description of the invention
The invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
A kind of blood cell of the present invention and analysis of biochemical instrument, including shift module, first hemolytic agent add module,
Second hemolytic agent add module, diluent add module, abluent add module, cleaning module, mixing module, control module,
First detection cup, the second detection cup and the 3rd detection cup；
Described shift module is used for shifting biochemical reagents and sample, including biochemical reagents position, sample position, suction needle, first
Liquid extracting device, connecting line and mobile device；Described mobile device is used for driving suction needle at sample position, biochemical reagents
Move between position, the first detection cup, the second detection cup and the 3rd detection cup；
Described first hemolytic agent adds module and includes the first hemolytic agent position and second liquid draw-out device, for the first inspection
Survey in cup and add the first hemolytic agent；
Described second hemolytic agent adds module and includes the second hemolytic agent position and the 5th liquid extracting device, for the second inspection
Survey in cup and add the second hemolytic agent；
Described diluent adds module and includes a dilution liquid level and the 3rd liquid extracting device, for the first detection
Cup, the second detection cup, the 3rd detection cup, suction needle and suction needle washer inject diluent；
Described abluent adds module and includes an abluent position and the 4th liquid extracting device, for the first detection cup
Abluent is added with in the second detection cup；
Described cleaning module includes suction needle washer, connecting line, waste liquid discharge pipe and waste drains pump, described suction needle
Washer is used for cleaning suction needle outer wall, and it is clear that described waste liquid discharge pipe is used for discharging waste liquid and suction needle washer in detection cup
The waste liquid produced after washing, described waste drains pump is discharged for waste liquid and is provided power；
Described mixing module is by air-blowing bottom first, second, and third detection cup, to the aqueous agitation in detection cup
Mixing；
It is respectively arranged with on the wall of cup of the first detection cup, the second detection cup and the 3rd detection cup and is provided with the first grain count
Detection device, the second grain count detection device and the 3rd grain count detection device, the first grain count detection device and the
Two grain count detection devices for carrying out nucleated cell detection to liquid in cup, and the 3rd grain count detection device is for red
Cell and hematoblastic quantity and volume detect；One of them detection cup of first detection cup and the second detection cup is arranged
Having transmission optical detection device, detect for hemoglobin, in the first detection cup and the second detection cup, at least one detection cup is arranged
There is biochemical detection device, after being used for adding biochemical reagents, carry out biochemical project detection；
Described control module in order to control shift module, diluent adds module, the first hemolytic agent adds module, second molten
Module, abluent interpolation module, cleaning module, mixing module, the first detection cup, the second detection cup and the 3rd detection are added in blood agent
Cup work, and perform to detect Data Analysis Services, show, print, output detections result and and external communication.
The present invention also provides for a kind of blood cell and analysis of biochemical instrument, adds including shift module, the first hemolytic agent
Module, the second hemolytic agent add module, the 3rd hemolytic agent adds module, diluent adds module, abluent adds module, cleaning
Module, mixing module, control module, the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup；
Described shift module is used for shifting biochemical reagents and sample, including biochemical reagents position, sample position, suction needle, first
Liquid extracting device, connecting line and mobile device；Described mobile device is used for driving suction needle at sample position, biochemical reagents
Move between position, the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup；
Described first hemolytic agent adds module and includes the first hemolytic agent position and second liquid draw-out device, for the first inspection
Survey in cup and add the first hemolytic agent；
Described second hemolytic agent adds module and includes the second hemolytic agent position and the 5th liquid extracting device, for the second inspection
Survey in cup and add the second hemolytic agent；
Described 3rd hemolytic agent adds module and includes the 3rd hemolytic agent position and the 6th liquid extracting device, for the 4th inspection
Survey and cup adds the 3rd hemolytic agent；
Described diluent adds module and includes a dilution liquid level and the 3rd liquid extracting device, for the first detection
Cup, the second detection cup, the 3rd detection cup, the 4th detection cup, suction needle and suction needle washer inject diluent；
Described abluent adds module and includes an abluent position and the 4th liquid extracting device, for the first detection
Cup, the second detection cup and the 4th detection cup add abluent；
Described cleaning module includes suction needle washer, connecting line, waste liquid discharge pipe and waste drains pump, described suction needle
Washer is used for cleaning suction needle outer wall, and it is clear that described waste liquid discharge pipe is used for discharging waste liquid and suction needle washer in detection cup
The waste liquid produced after washing, described waste drains pump is discharged for waste liquid and is provided power；
Described mixing module is by air-blowing bottom first, second, third and fourth detection cup, to the liquid in detection cup
Agitation mixing；
It is respectively arranged with on the wall of cup of the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup and is provided with
First grain count detection device, the second grain count detection device, the 3rd grain count detection device and the 4th grain count
Detection device, the first grain count detection device, the second grain count detection device and the 4th grain count detection device are used for
Liquid in cup carries out nucleated cell detection, and the 3rd grain count detection device is for erythrocyte and hematoblastic quantity and body
Amass and detect；One of them detection cup of first detection cup, the second detection cup and the 4th detection cup is provided with the inspection of transmission light
Surveying device, detect for hemoglobin, in the first detection cup, the second detection cup and the 4th detection cup, at least one detection cup is arranged
There is biochemical detection device, after being used for adding biochemical reagents, carry out biochemical project detection；
Described control module in order to control shift module, diluent adds module, the first hemolytic agent adds module, second molten
Blood agent add module, the 3rd hemolytic agent add module, abluent add module, cleaning module, mixing module, first detection cup,
Second detection cup, the 3rd detection cup and the 4th detection cup work, and perform to detect Data Analysis Services, show, print, export inspection
Survey result and and external communication.
Further, the first detection cup wall of cup is provided with transmission optical detection device and the first biochemical detection device, second
It is provided with the second biochemical detection device on detection cup wall of cup, the 4th detection cup wall of cup is provided with the 3rd biochemical detection device.
Further, described biochemical detection device include transmission optical detection device and scattering optical detection device in one or
Two kinds.
Further, analyser also includes the washer for cleaning suction needle outer wall.
Further, described scattering optical detection device and transmission optical detection device are multi-wavelength detection device.
Instrument needs wavelength switching according to detection, and the switching of detection wavelength is by toggle lights, switching voltage or detector
Front installation can change the monochromator device of wavelength and realize.
Further, analyser also includes a Washing cup, for being carried out suction needle inside and outside.
The present invention also provides for a kind of analysis method based on blood cell and analysis of biochemical instrument, and the method includes following
Step:
Step 1, in specimen cup, quantitatively draw whole blood sample by suction needle, quantitatively transfer to the first detection cup, second
Detection cup and the 4th detects in cup, and diluent adds module difference in the first detection cup, the second detection cup and the 4th detection cup
Add quantitative diluent, and by mixing module mixing the first detection cup, the second detection cup and the 4th detection cup；By inhaling sample
Suction needle outer wall is carried out by pin washer；
Step 2, in the first, second, the 4th detection any one cup of cup, draw quantitative dilution, mixing by suction needle after
Blood sample be transferred to the 3rd detection cup, mix with the diluent in cup, mixing module detects liquid blending in cup to the 3rd, the
Three grain count detection devices carry out erythrocyte and platelets analysis to blood sample after mixing in the 3rd detection cup；Clear by suction needle
Wash device suction needle outer wall is carried out；
Step 3, module of being added by the first hemolytic agent add the first quantitative hemolytic agent and through mixed in the first detection cup
Even module is to liquid blending in the first detection cup, and the first grain count detection device and transmission optical detection device are respectively to the first inspection
After surveying cup mixing, the nucleated cell in blood sample and hemoglobin detect；
Step 4, by the second hemolytic agent adding set to second detection cup in add the second hemolytic agent, the second grain count
Nucleated cell is detected by detection device；
Step 5, by the 3rd hemolytic agent adding set to the 4th detection cup in add the 3rd hemolytic agent, the 4th grain count
Nucleated cell is detected by detection device；
Suction needle after step 6, cleaning moves to biochemical reagents position, draws the first quantitative biochemical reagents and is transferred to
First detection cup, liquid in mixing the first detection cup, is mixed after adding biochemical reagents by the first biochemical detection device again
Dilute sample detect, it is thus achieved that the testing result of corresponding biochemical project；
Suction needle after step 7, cleaning moves to the second biochemical reagents position again, draws quantitative the second biochemical reagents and adds
To the second detection cup, perform the second biochemical project by the second biochemical detection device and detect；Suction needle after cleaning is quantitatively inhaled
Take the third biochemical reagents, add the 4th detection cup, perform the third biochemical project by the 3rd biochemical detection device and detect.
Instrument is added module carried out each detection cup, suction needle clearly by cleaning module, diluent after completing all to detect
Washing, instrument completes this testing process.
In the present invention, nucleated cell includes basophilic granulocyte, eosinophilic granulocyte, lymphocyte, mononuclear cell, neutrality
Granulocyte detection and reticulocyte.The determination method of nucleated cell can be that electrical impedance method, electrical impedance combine sheath streamer
Learn detection method or simple cell dyeing sheath Flow Technique detection method.
Detecting discrepant biochemistry detecting item for whole blood sample with serum/plasma, instrument can be automatically to each blood sample
Biochemical component testing result is modified, it is ensured that this instrument each biochemistry detection result and real biochemical component or the life of standard
Changing testing result consistent, do not have discrepant biochemical project for serum/plasma detection with whole blood test, instrument is without revising detection
Result.The principle revised is owing to the biochemistry detection of standard is the biochemistry ingredient content in detection plasma/serum, and contains in whole blood
There is a large amount of Cell Component, and part biochemical component there are differences at intraor extracellular；Part biochemical component only/be primarily present in blood
Liquid is intracellular；Part biochemical component is primarily present in outside blood cell, therefore whole blood sample biochemistry ingredient content and plasma/serum
There may be difference；In addition, when in whole blood, erythrocyte, leukocyte, platelet composition also assist in biochemical project detection, this part becomes
The detection of corresponding biochemical project also can be caused certain impact by part.Therefore whole blood sample biochemistry detection result and the life of plasma/serum
Change testing result and there is error.
For eliminating these errors, instrument of the present invention to testing result according to equation below correction:
Wherein, serum/plasma volume=volume of whole blood-cell volume, platelet counts × blood in cell volume=whole blood
In platelet average external volume+whole blood, in erythrocyte number × mean corpuscular volume+whole blood, quantity of leucocyte × whole leukocyte are put down
All volume × modifying factors b, the scope of modifying factor a and b is respectively 0.3-4.0.In the present invention, "/" represents the implication of "or".
The volume of leukocyte can calculate according to equation below:
(1) leukocyte volume=big leukocyte average external volume × big quantity of leucocyte × modifying factor c+ SL volume
× SL quantity × modifying factor d, modifying factor c and d are in the range of respectively 0.3-4.0.
(2) leukocyte volume=lymphocyte average external volume × lymphocyte quantity × modifying factor e+ neutrophilic granulocyte body
Long-pending × neutrophilic granulocyte quantity × modifying factor f+ intermediate cell volume × intermediate cell quantity × modifying factor g, modifying factor
E-g is in the range of respectively 0.3-4.0.
The sample position of blood cell and analysis of biochemical instrument and method, respectively detect cup, biochemical reagents position is all designed and is being inhaled
In sample pin motion track, Fig. 1-1 to Fig. 1-5 is several concrete layout type:
Fig. 1-1: sample needle motion track is that Cs → the first biochemical reagents position, sample position R1 → the first detects cup C1 → the second
Detection cup C2 → 3rd detects cup C3；
Fig. 1-2: sample needle motion track be Cs → the first biochemical reagents position, sample position R1 → the second biochemical reagents position R1 ' →
First detection cup C1 → the second detection cup C2 → 4th detection cup C4 → 3 detects cup C3；
Fig. 1-3: sample needle motion track be Cs → the first biochemical reagents position, sample position R1 → the second biochemical reagents position R1 ' →
3rd biochemical reagents position R1 " → the first detection cup C1 → the second detection cup C2 → 4th detection cup C4 → 3rd detection cup C3；
Fig. 1-4 and Fig. 1-5: sample needle motion track is sample position Cs → the first detection cup C1 → the second detection cup C2 → the
Four detection cup C4 → three detect cup C3 → the first biochemical reagents position R1 → the second biochemical reagents position R1 ' → the 3rd biochemical reagents position
R1”。
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
The present embodiment, as a example by four detection cups, detects routine blood test and single reagent biochemical project.
As in figure 2 it is shown, the first detection cup C1 is provided with the first grain count detection device D4, the first transmission optical detection device
With the first biochemical detection device, the first biochemical detection device includes the first scattering optical detection device；First transmission optical detection device
Including the first transmission radiant L1；First scattering optical detection device includes the first scattered light light source L4, the first scattered light detection dress
Put and share the first detector D1 with the first transmission optical detection device.
Second detection cup C2 is provided with the second grain count detection device D5 and the second biochemical detection device, the second biochemical inspection
Survey device and include the second transmission optical detection device and the second scattering optical detection device.Second transmission optical detection device and the second scattering
Optical detection device selects one of them unlatching to carry out biochemical project detection；Second transmission optical detection device includes the second transmission light light
Source L2, the second scattering optical detection device includes the second scattered light light source L5, the second scattering optical detection device and the inspection of the second transmission light
Survey device and share the second detector D2；
4th detection cup C4 is provided with the 4th grain count detection device D6 and the 3rd biochemical detection device, and the described 3rd is biochemical
Detection device includes the 3rd transmission optical detection device and the 3rd scattering optical detection device, and the 3rd transmission optical detection device and the 3rd dissipates
Penetrating optical detection device selects one of them unlatching to carry out biochemical project detection；3rd transmission optical detection device includes the 3rd transmission light
Light source L3, the 3rd scattering optical detection device includes the 3rd scattered light light source L6, the 3rd transmission optical detection device and the 3rd scattered light
Detection device shares the 3rd detector D3；
3rd detection cup C3 is provided with the 3rd grain count detection device D7.
As shown in Figure 4, suction needle N1 moves in specimen cup, and the second valve V2 closes, the first valve V1 opens, the first liquid
Body draw-out device S1 piston is pulled back, and quantitative sample sucks in suction needle N1, and mobile device drives N1 to move to the first detection cup C1,
Second valve V2 closes, and the first valve V1 opens, and first liquid draw-out device S1 piston advances and added by blood sample in suction needle N1
In the first detection cup C1；According to same method, suction needle N1 moves to the second detection cup C2 and the 4th detection cup C4, will be fixed
Amount blood sample is separately added into the second detection cup C2 and the 4th detection cup C4；Diluent adds the 6th valve V6 in module and opens, and second
Valve V2, the 24th valve V24, the 20th valve V20, the 21st valve V21 and the 5th valve V5 close, the 3rd liquid
Draw-out device S3 piston is pulled back and is sucked in the 3rd liquid extracting device S3 by diluent R3, and the 6th valve V6 closes, the 20th valve
Door V20, the 21st valve V21, the 24th valve V24 open, and the 3rd liquid extracting device S3 piston advances, the most former suction
Diluent in 3rd liquid extracting device S3 is along the first diluent pin N31Enter the first detection cup C1, along the second diluent pin
N32Enter the second detection cup C2, along the 3rd diluent pin N33Enter the 4th detection cup C4；Mixing module in the tenth valve V10 and
12nd valve V12 closes, and the 9th valve V9, the 22nd valve V22, the 23rd valve V23 and the 11st valve V11 open
Open, air pump P1 air-blowing, drive gas to enter at the bottom of the first detection cup C1, the second detection cup C2 and the 4th detection cup C4 along pipeline
Portion, liquid in mixing cup.
Suction needle N1 according to previous mode in the first detection cup C1, draw the diluted sample after part mixing, be transferred to the
Three detection cup C3, diluent adds the 6th valve V6 in module and opens, the second valve V2, the 24th valve V24, the 20th valve
Door V20, the 21st valve V21 and the 5th valve V5 closes, the 3rd liquid extracting device S3 piston pull back draw quantitative dilute
Release liquid R3, the 6th valve V6, the 24th valve V24, the 20th valve V20 and the 21st valve V21 to close, the 5th valve
V5, the first valve V1 and the second valve V2 open, and the 3rd liquid extracting device S3 piston advances the 3rd liquid extracting device S3
In quantitative diluent by the 4th diluent pin pin N3 inject the 3rd detection cup C3, simultaneously by pipeline enter suction needle N1,
It is carried out inside suction needle N1.Mixing module in the 9th valve V9, the 22nd valve V22, the 23rd valve V23 and
12nd valve V12 closes, and the tenth valve V10 and the 11st valve V11 opens, air pump P1 air-blowing, drives gas along pipeline
Enter bottom the 3rd detection cup C3, liquid in mixing cup.3rd grain count detection device D7 is to sample in the 3rd detection cup C3
Platelet and erythrocytic quantity and volume detect.
The outer washer of pin is carried out outside suction needle N1.4th valve V4 opens afterwards, and the 3rd valve V3 closes, the
Two liquid extracting device S2 pull back, and the first hemolytic agent R2 sucks in second liquid draw-out device S2, and the 4th valve V4 closes, and the 3rd
Valve V3 opens, and second liquid draw-out device S2 piston advances, and makes quantitative first hemolytic agent enter the first detection cup C1.Mixing mould
In block, the 11st valve V11 and the 9th valve V9 opens, the tenth valve V10, the 22nd valve V22, the 23rd valve V23
Close with the 12nd valve V12, air pump P1 air-blowing, drive gas to enter bottom the first detection cup C1 along pipeline, in mixing cup
Liquid.In first detection cup C1, nucleated cell in cup is detected by the first grain count detection device D4, the first transmission light light
Source L1 opens, and content of hemoglobin is detected by the first detector D1.
Suction needle N1 moves to the first biochemical reagents position R1, and suction needle N1 gos deep in the first biochemical reagents, at the first liquid
Under body draw-out device S1, the first valve V1 and the second valve V2 coordinate, raw by the first biochemical reagents position R1 draws quantitative first
Changing reagent, suction needle N1 moves to the first detection cup C1 afterwards, the first biochemical reagents adds the first detection cup C1, mixes mould
In block, the 11st valve V11 and the 9th valve V9 opens, the tenth valve V10, the 22nd valve V22, the 23rd valve V23
Close with the 12nd valve V12, air pump P1 air-blowing, drive gas to enter bottom the first detection cup C1 along pipeline, in mixing cup
Liquid.As Fig. 2, the first scattered light light source L4 open, the first transmission radiant L1 closes, the first detector D1 detection scattered light letter
Number, it is thus achieved that single reagent biochemical project testing result.
The outer washer of pin is carried out outside suction needle N1.13rd valve V13 opens afterwards, the 14th valve V14
Closing, the 5th liquid extracting device S5 pulls back, and the second hemolytic agent R5 sucks in the 5th liquid extracting device S5, the 13rd valve
V13 closes, and the 14th valve V14 opens, and the 5th liquid extracting device S5 piston advances, and makes quantitative second hemolytic agent enter second
Detection cup C2.In mixing module, the 11st valve V11 and the 22nd valve V22 opens, the 9th valve V9, the 23rd valve
V23, the tenth valve V10 and the 12nd valve V12 close, air pump P1 air-blowing, drive gas to enter the second detection cup along pipeline
Bottom C2, liquid in mixing cup.Part nucleated cell in cup is detected by the second grain count detection device D5.
15th valve V15 opens afterwards, and the 16th valve V16 closes, and the 6th liquid extracting device S6 pulls back, and the 3rd is molten
Blood agent R6 sucks in the 6th liquid extracting device S6, and the 15th valve V15 closes, and the 16th valve V16 opens, and the 6th liquid is taken out
Fetching is put S6 piston and is advanced, and makes quantitative 3rd hemolytic agent enter the 4th detection cup C4.11st valve V11 and the in mixing module
23 valve V23 open, and the 9th valve V9, the 22nd valve V22, the tenth valve V10 and the 12nd valve V12 close,
Air pump P1 air-blowing, drives gas to enter bottom the 4th detection cup C4 along pipeline, liquid in mixing cup.4th grain count detection
Part nucleated cell in cup is detected by device D6.
After detection terminates, open the 9th valve V9, the 22nd valve V22, the 23rd valve V23, the tenth valve V10
Bleed with the 12nd valve V12, waste drains pump P2, enter waste liquid receipts by each detection cup, the outer washer of pin and pipeline detect waste liquid
In storage；The 8th valve V8 during abluent adds module afterwards opens, and the 7th valve V7 closes, the 4th liquid extracting device S4
Pulling back, abluent R4 sucks the 4th liquid extracting device S4, and the 8th valve V8 closes afterwards, the 7th valve V7, the 17th valve
V17, the 18th valve V18 and the 19th valve V19 open, and the 4th liquid extracting device S4 advances, and abluent R4 passes through first
Abluent pin N4 enters the first detection cup C1, by the second abluent pin N41Enter the second detection cup C2, by the 3rd abluent
Pin N42Enter the 4th detection cup C4, open the 9th valve V9 afterwards, the 22nd valve V22, the 23rd valve V23 and the
12 valve V12, waste drains pump P2 bleed, and clean waste liquid and enter liquid waste collector.
Diluent adds the 6th valve V6 of module and opens, the second valve V2, the 24th valve V24, the 20th valve
V20, the 21st valve V21 and the 5th valve V5 close, and the 3rd liquid extracting device S3 piston is pulled back and drawn quantitative dilution
Liquid R3, the 6th valve V6 closes afterwards, the 5th valve V5, the 20th valve V20, the 21st valve V21, the 24th valve
V24, the first valve V1 and the second valve V2 open, and the 3rd liquid extracting device S3 piston advances the 3rd liquid extracting device S3
In quantitative diluent by the 4th diluent pin N3 enter the 3rd detection cup C3, by the first diluent pin N31Enter first
Detection cup C1, by the second diluent pin N32Enter the second detection cup C2, by the 3rd diluent pin N33Enter the 4th detection cup
In C4, enter suction needle N1 by pipeline simultaneously, be carried out inside each detection cup and suction needle N1.Suction needle N1 is the most also
Perform pin exterior washings, clean and after terminating, open the 9th valve V9, the 22nd valve V22, the 23rd valve V23, the tenth
Valve V10 and the 12nd valve V12, waste drains pump P2 bleed, and clean waste liquid and enter liquid waste collector.
Embodiment 2
In conjunction with Fig. 2 and Fig. 4, the analyser of the present embodiment is with the difference of embodiment 1, detects routine blood test in the present embodiment
With double reagent biochemical project.
Suction needle N1 moves to the first biochemical reagents position R1, and suction needle N1 gos deep in the first biochemical reagents, at the first liquid
Under body draw-out device S1, the first valve V1 and the second valve V2 coordinate, drawing quantitative first biochemical reagents, suction needle N1 moves afterwards
Move to the first detection cup C1 place, the first biochemical reagents are added the first detection cup C1, mix in module the 11st valve V11 and the
Nine valve V9 open, and the tenth valve V10, the 22nd valve V22, the 23rd valve V23 and the 12nd valve V12 close,
Air pump P1 air-blowing, drives gas to enter bottom the first detection cup C1 along pipeline, liquid in mixing cup.Suction needle N1 moves afterwards
To the second R1 ' place, biochemical reagents position, draw quantitative second biochemical reagents according to the same manner and be transferred to the first detection cup C1, pressing
After liquid in aforesaid way mixing cup, the first scattered light light source L4 opens, and the first transmission radiant L1 closes, the first detector
D1 detects scattered light signal, it is thus achieved that the testing result of double reagent biochemical project.
Embodiment 3
The present embodiment is with the difference of embodiment 1 and embodiment 2, and the first transmission radiant L1 opens, the first scattered light
Light source L4 closes, and the first detector D1 detects optical signal transmissive, it is thus achieved that biochemical project testing result.
Embodiment 4
In conjunction with Fig. 3-1, Fig. 3-2 and Fig. 3-3, the analyser of the present embodiment is with the difference of embodiment 1, the present embodiment
First transmitted light source L1, the second transmitted light source L2 and the 3rd transmitted light source L3 arrange multiple wavelength, by changing the first optical filter
F1, the second optical filter F2 and the 3rd optical filter F3 obtain the transmission light of different wave length, for different biochemical project detections.Described
One optical filter F1, the wavelength difference of the second optical filter F2 and the 3rd optical filter F3.
Embodiment 5
In conjunction with Fig. 3-1, Fig. 3-2 and Fig. 3-3, the analyser of the present embodiment is with the difference of embodiment 1, the present embodiment
First scattering light source L4, the second scattering light source L5 and the 3rd scattering light source L6 arrange multiple wavelength, by changing the first optical filter
F1, the second optical filter F2 and the 3rd optical filter F3 obtain the scattered light of different wave length, for different biochemical project detections.
Embodiment 6
The present embodiment uses existing biochemical instruments to detect CRP and the HbAle of 20 different samples from analyser of the present invention
Albumen result carries out dependency and compares, statistical data such as table 1.
The different sample of table 1 compares with analyser testing result of the present invention by existing biochemical instruments respectively
It is respectively adopted existing biochemical instruments from analyser of the present invention, 20 different samples to be detected, and carries out dependency and divide
Analysis, as shown in Figure 5 and Figure 6, two kinds of distinct methods testing result dependency: RCRP=0.9974 >=0.95, RHbA1C=0.9783
>=0.95, illustrate that the testing result dependency of existing biochemical instruments and analyser of the present invention is good.
Claims (10)
1. a blood cell and analysis of biochemical instrument, it is characterised in that include that shift module, the first hemolytic agent add mould
Block, the second hemolytic agent add module, diluent adds module, abluent interpolation module, cleaning module, mixing module, control mould
Block, the first detection cup, the second detection cup and the 3rd detection cup；
Described shift module is used for shifting biochemical reagents and sample, including biochemical reagents position, sample position, suction needle, first liquid
Draw-out device, connecting line and mobile device；Described mobile device is used for driving suction needle in sample position, biochemical reagents position,
Move between one detection cup, the second detection cup and the 3rd detection cup；
Described first hemolytic agent adds module and includes the first hemolytic agent position and second liquid draw-out device, for the first detection cup
Middle interpolation the first hemolytic agent；
Described second hemolytic agent adds module and includes the second hemolytic agent position and the 5th liquid extracting device, for the second detection cup
Middle interpolation the second hemolytic agent；
Described diluent adds module and includes a dilution liquid level and the 3rd liquid extracting device, for the first detection cup, the
Two detection cups, the 3rd detection cup, suction needle and suction needle washer inject diluent；
Described abluent adds module and includes an abluent position and the 4th liquid extracting device, for the first detection cup and the
Two detection cups add abluent；
Described cleaning module includes suction needle washer, connecting line, waste liquid discharge pipe and waste drains pump, and described suction needle cleans
Device is used for cleaning suction needle outer wall, and described waste liquid discharge pipe is used for discharging after in detection cup, waste liquid and suction needle washer clean
The waste liquid produced, described waste drains pump is discharged for waste liquid and is provided power；
Described mixing module is by air-blowing bottom first, second, and third detection cup, to the aqueous agitation mixing in detection cup；
First detection cup, second detection cup and the 3rd detection cup wall of cup on be respectively arranged with first grain count detection device,
Second grain count detection device and the 3rd grain count detection device, the first grain count detection device and the second grain count
Detection device for carrying out nucleated cell detection to liquid in cup, and the 3rd grain count detection device is for little to erythrocyte and blood
Quantity and the volume of plate detect；One of them detection cup of first detection cup and the second detection cup is provided with the inspection of transmission light
Surveying device, detect for hemoglobin, in the first detection cup and the second detection cup, at least one detection cup is provided with biochemistry detection
Device, carries out biochemical project detection after being used for adding biochemical reagents；
Described control module is in order to control shift module, diluent interpolation module, the first hemolytic agent interpolation module, the second hemolytic agent
Add module, abluent interpolation module, cleaning module, mixing module, the first detection cup, the second detection cup and the 3rd detection cup work
Make, and perform to detect Data Analysis Services, show, print, output detections result and and external communication.
2. a blood cell and analysis of biochemical instrument, it is characterised in that include that shift module, the first hemolytic agent add mould
Block, the second hemolytic agent add module, the 3rd hemolytic agent adds module, diluent adds module, abluent adds module, clean mould
Block, mixing module, control module, the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup；
Described shift module is used for shifting biochemical reagents and sample, including biochemical reagents position, sample position, suction needle, first liquid
Draw-out device, connecting line and mobile device；Described mobile device is used for driving suction needle in sample position, biochemical reagents position,
Move between one detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup；
Described first hemolytic agent adds module and includes the first hemolytic agent position and second liquid draw-out device, for the first detection cup
Middle interpolation the first hemolytic agent；
Described second hemolytic agent adds module and includes the second hemolytic agent position and the 5th liquid extracting device, for the second detection cup
Middle interpolation the second hemolytic agent；
Described 3rd hemolytic agent adds module and includes the 3rd hemolytic agent position and the 6th liquid extracting device, for the 4th detection cup
Middle interpolation the 3rd hemolytic agent；
Described diluent adds module and includes a dilution liquid level and the 3rd liquid extracting device, for the first detection cup, the
Two detection cups, the 3rd detection cup, the 4th detection cup, suction needle and suction needle washer inject diluent；
Described abluent adds module and includes an abluent position and the 4th liquid extracting device, for the first detection cup, the
Two detection cups and the 4th detection cup add abluent；
Described cleaning module includes suction needle washer, connecting line, waste liquid discharge pipe and waste drains pump, and described suction needle cleans
Device is used for cleaning suction needle outer wall, and described waste liquid discharge pipe is used for discharging after in detection cup, waste liquid and suction needle washer clean
The waste liquid produced, described waste drains pump is discharged for waste liquid and is provided power；
Described mixing module is by air-blowing bottom first, second, third and fourth detection cup, to the aqueous agitation in detection cup
Mixing；
It is respectively arranged with on the wall of cup of the first detection cup, the second detection cup, the 3rd detection cup and the 4th detection cup and is provided with first
Grain count detection device, the second grain count detection device, the 3rd grain count detection device and the 4th grain count detection
Device, the first grain count detection device, the second grain count detection device and the 4th grain count detection device are for cup
Middle liquid carries out nucleated cell detection, and the 3rd grain count detection device is for entering erythrocyte and hematoblastic quantity and volume
Row detection；One of them detection cup of first detection cup, the second detection cup and the 4th detection cup is provided with transmission light detection dress
Putting, detect for hemoglobin, in the first detection cup, the second detection cup and the 4th detection cup, at least one detection cup is provided with life
Change detection device, after being used for adding biochemical reagents, carry out biochemical project detection；
Described control module is in order to control shift module, diluent interpolation module, the first hemolytic agent interpolation module, the second hemolytic agent
Add module, the 3rd hemolytic agent add module, abluent add module, cleaning module, mixing module, first detection cup, second
Detection cup, the 3rd detection cup and the 4th detection cup work, and perform to detect Data Analysis Services, show, print, output detections is tied
Fruit and and external communication.
Blood cell the most according to claim 2 and analysis of biochemical instrument, it is characterised in that on the first detection cup wall of cup
It is provided with transmission optical detection device and the first biochemical detection device, the second detection cup wall of cup is provided with the second biochemistry detection dress
Put, the 4th detection cup wall of cup is provided with the 3rd biochemical detection device.
4. according to the blood cell described in claim 1,2 or 3 and analysis of biochemical instrument, it is characterised in that described biochemical inspection
Survey device includes one or both in transmission optical detection device and scattering optical detection device.
Blood cell the most according to claim 1 and 2 and analysis of biochemical instrument, it is characterised in that analyser also includes
For cleaning the washer of suction needle outer wall.
Blood cell the most according to claim 1 and 2 and analysis of biochemical instrument, it is characterised in that described scattered light is examined
Survey device and transmission optical detection device is multi-wavelength detection device.
Blood cell the most according to claim 1 and 2 and analysis of biochemical instrument, it is characterised in that analyser also includes
One Washing cup, for being carried out suction needle inside and outside.
8. an analysis method based on blood cell described in claim 3 and analysis of biochemical instrument, it is characterised in that the party
Method comprises the following steps:
Step 1, in specimen cup, quantitatively draw whole blood sample by suction needle, quantitatively transfer to the first detection cup, the second detection
In cup and the 4th detection cup, diluent adds module and is separately added in the first detection cup, the second detection cup and the 4th detection cup
Quantitative diluent, and by mixing module mixing the first detection cup, the second detection cup and the 4th detection cup；Clear by suction needle
Wash device suction needle outer wall is carried out；
Step 2, in the first, second, the 4th detection any one cup of cup, drawn the blood after quantitative dilution, mixing by suction needle
Sample is transferred to the 3rd detection cup, mix with the diluent in cup, mixing module detect liquid blending in cup to the 3rd, the 3rd
Grain counting and detecting device carries out erythrocyte and platelets analysis to blood sample after mixing in the 3rd detection cup；By suction needle washer
Suction needle outer wall is carried out；
Step 3, module of being added by the first hemolytic agent add the first quantitative hemolytic agent and through mixing mould in the first detection cup
Block is to liquid blending in the first detection cup, and the first grain count detection device and transmission optical detection device are respectively to the first detection cup
After mixing, the nucleated cell in blood sample and hemoglobin detect；
Step 4, in the second detection cup, add the second hemolytic agent by the second hemolytic agent adding set, the second grain count detection
Nucleated cell is detected by device；
Step 5, by the 3rd hemolytic agent adding set to the 4th detection cup in add the 3rd hemolytic agent, the 4th grain count detection
Nucleated cell is detected by device；
Suction needle after step 6, cleaning moves to biochemical reagents position, draws the first quantitative biochemical reagents and is transferred to first
Detection cup, liquid in mixing the first detection cup, dilute to mix after adding biochemical reagents by the first biochemical detection device again
Release sample to detect, it is thus achieved that the testing result of corresponding biochemical project；
Step 7, clean after suction needle move to the second biochemical reagents position again, draw quantitative the second biochemical reagents and add to the
Two detection cups, perform the second biochemical project by the second biochemical detection device and detect；Suction needle after cleaning quantitatively draws
Three kinds of biochemical reagents, add the 4th detection cup, perform the third biochemical project by the 3rd biochemical detection device and detect.
The analysis method of blood cell the most according to claim 8 and analysis of biochemical instrument, it is characterised in that described life
Change detection device includes one or both in transmission optical detection device and scattering optical detection device.
The analysis method of blood cell the most according to claim 8 and analysis of biochemical instrument, it is characterised in that instrument
Automatically being modified biochemistry detection result during detection, modified computing formulae is as follows:
Wherein modifying factor a is for revising the error that other influence factor causes, in the range from 0.3-4.0.
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| CN201610681443.0A CN106124751B (en) | 2016-08-17 | 2016-08-17 | Blood cell and analysis of biochemical instrument and method |
| PCT/CN2017/070576 WO2018032720A1 (en) | 2016-08-17 | 2017-01-08 | Blood cell and biochemical content analyzer and method |
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| WO2018032718A1 (en) * | 2016-08-17 | 2018-02-22 | 江苏英诺华医疗技术有限公司 | Blood cell and biochemical assay device, and assay method thereof |
| WO2018032720A1 (en) * | 2016-08-17 | 2018-02-22 | 江苏英诺华医疗技术有限公司 | Blood cell and biochemical content analyzer and method |
| CN107966579A (en) * | 2017-12-26 | 2018-04-27 | 江苏柯伦迪医疗技术有限公司 | One kind has biochemistry, enzyme is exempted from and chemiluminescence rapid detection system and method |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101169450A (en) * | 2006-10-27 | 2008-04-30 | 深圳迈瑞生物医疗电子股份有限公司 | Full-automatic biochemical analysis method and device |
| US20100105074A1 (en) * | 2008-10-27 | 2010-04-29 | Nodality, Inc. A Delaware Corporation | High throughput flow cytometry system and method |
| CN103543256A (en) * | 2012-07-12 | 2014-01-29 | 三星电子株式会社 | Inspection unit and fluid analysis cartridge |
| CN103792376A (en) * | 2012-10-30 | 2014-05-14 | 希森美康株式会社 | Sample analyzer |
| CN104122404A (en) * | 2014-08-01 | 2014-10-29 | 江苏英诺华医疗技术有限公司 | Novel rapid multi-parameter platelet functional analyzer and detection method thereof |
| WO2015054695A2 (en) * | 2013-10-11 | 2015-04-16 | Immunetics, Inc. | Led assay reader with touchscreen control and barcode sample id |
| CN104833813A (en) * | 2015-05-12 | 2015-08-12 | 江苏英诺华医疗技术有限公司 | Analyzer with blood routine and biochemical detection functions |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0905514B1 (en) * | 1997-09-27 | 2003-11-26 | Horiba, Ltd. | Blood cell count/immunoassay apparatus using whole blood |
| CN103336130B (en) * | 2013-06-21 | 2016-03-16 | 嘉善加斯戴克医疗器械有限公司 | A kind of whole blood immunity analytical equipment and use the blood analyser of this device |
| CN105334333B (en) * | 2014-07-01 | 2019-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of whole blood measuring instrument and automatic sampling apparatus being easily changed latex reagent |
| CN105699380A (en) * | 2016-03-23 | 2016-06-22 | 深圳市帝迈生物技术有限公司 | Analysis equipment and method for simultaneously measuring CRP (C-Reactive Protein) and blood routine |
| CN106124751B (en) * | 2016-08-17 | 2018-05-18 | 江苏英诺华医疗技术有限公司 | Blood cell and analysis of biochemical instrument and method |
-
2016
- 2016-08-17 CN CN201610681443.0A patent/CN106124751B/en active Active
-
2017
- 2017-01-08 WO PCT/CN2017/070576 patent/WO2018032720A1/en active Application Filing
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101169450A (en) * | 2006-10-27 | 2008-04-30 | 深圳迈瑞生物医疗电子股份有限公司 | Full-automatic biochemical analysis method and device |
| US20100105074A1 (en) * | 2008-10-27 | 2010-04-29 | Nodality, Inc. A Delaware Corporation | High throughput flow cytometry system and method |
| CN103543256A (en) * | 2012-07-12 | 2014-01-29 | 三星电子株式会社 | Inspection unit and fluid analysis cartridge |
| CN103792376A (en) * | 2012-10-30 | 2014-05-14 | 希森美康株式会社 | Sample analyzer |
| WO2015054695A2 (en) * | 2013-10-11 | 2015-04-16 | Immunetics, Inc. | Led assay reader with touchscreen control and barcode sample id |
| CN104122404A (en) * | 2014-08-01 | 2014-10-29 | 江苏英诺华医疗技术有限公司 | Novel rapid multi-parameter platelet functional analyzer and detection method thereof |
| CN104833813A (en) * | 2015-05-12 | 2015-08-12 | 江苏英诺华医疗技术有限公司 | Analyzer with blood routine and biochemical detection functions |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018032718A1 (en) * | 2016-08-17 | 2018-02-22 | 江苏英诺华医疗技术有限公司 | Blood cell and biochemical assay device, and assay method thereof |
| WO2018032720A1 (en) * | 2016-08-17 | 2018-02-22 | 江苏英诺华医疗技术有限公司 | Blood cell and biochemical content analyzer and method |
| CN106896049A (en) * | 2017-03-16 | 2017-06-27 | 江苏柯伦迪医疗技术有限公司 | A kind of multi-parameter blood analyser and method |
| CN107966579A (en) * | 2017-12-26 | 2018-04-27 | 江苏柯伦迪医疗技术有限公司 | One kind has biochemistry, enzyme is exempted from and chemiluminescence rapid detection system and method |
| WO2019127959A1 (en) * | 2017-12-26 | 2019-07-04 | 江苏英诺华医疗技术有限公司 | Rapid detection system and method involving biochemical detection, enzyme immunoassay and chemiluminescence detection |
| WO2019127958A1 (en) * | 2017-12-26 | 2019-07-04 | 江苏英诺华医疗技术有限公司 | Medical rapid biochemical detection system and detection method |
| CN110346573A (en) * | 2018-04-08 | 2019-10-18 | 深圳市帝迈生物技术有限公司 | Blood analyzing apparatus |
| CN113311163A (en) * | 2020-02-26 | 2021-08-27 | 深圳迈瑞生物医疗电子股份有限公司 | Cleaning method of detection pool |
| CN113311163B (en) * | 2020-02-26 | 2024-04-12 | 深圳迈瑞生物医疗电子股份有限公司 | Cleaning method of detection pool |
| CN113959992A (en) * | 2021-10-20 | 2022-01-21 | 深圳市科曼医疗设备有限公司 | Whole blood detection and analysis system and whole blood sample detection method |
| CN113959992B (en) * | 2021-10-20 | 2023-07-18 | 深圳市科曼医疗设备有限公司 | Whole blood detection analysis system and whole blood sample detection method |
| CN118067590A (en) * | 2024-04-17 | 2024-05-24 | 南京佰抗生物科技有限公司 | Instrument for blood analysis |
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