CN205538976U - Simple reliable five categorised analytical equipment of blood cell - Google Patents
Simple reliable five categorised analytical equipment of blood cell Download PDFInfo
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- CN205538976U CN205538976U CN201620085594.5U CN201620085594U CN205538976U CN 205538976 U CN205538976 U CN 205538976U CN 201620085594 U CN201620085594 U CN 201620085594U CN 205538976 U CN205538976 U CN 205538976U
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Abstract
The utility model discloses a simple reliable five categorised analytical equipment of blood cell, including sampling needle, white blood cell count(WBC) pond, weak solution, first hemolytic agent, second hemolytic agent, five categorised detection device, discharging equipment and transport modules, the transport module is connected to the sampling needle, through the distribution of transport module cooperation completion sample sample circuit, the weak solution is connected the white blood cell count(WBC) pond and is carried the module, first hemolytic agent is connected the white blood cell count(WBC) pond and is carried the module, the second hemolytic agent is connected the white blood cell count(WBC) pond and is carried the module, five categorised detection device connect the transport module, discharging equipment connects the white blood cell count(WBC) pond. Adopt the utility model discloses can realize with low costsly, small to basophil's accuracy can reach high -end five categorised hematology analyzer levels, more is applicable to basic -level hospital and uses.
Description
Technical field
The utility model relates to blood cell analysis field, a kind of simple and reliable haemocyte five
Classification analysis device.
Background technology
Haemocyte in blood of human body includes the compositions such as leucocyte, red blood cell, blood platelet, wherein leucocyte
It is divided into again this five class of lymphocyte, monocyte, basicyte, neutrophil leucocyte, eosinophil
Leucocyte, in hospital, blood routine examination is the inspection project counting haemocyte in blood.Before by
Limited in technology, cellanalyzer fails five class leucocytes are carried out Accurate classification counting, can only be by the thinnest
Born of the same parents are divided into three groups: lymphocyte populations, intermediate group cell, neutrophil leucocyte group, fail to be supplied to doctor complete
The diagnostic message in face and inconvenient doctor carries out clinical diagnosis.Therefore the haemocyte of leucocyte five classification can be carried out
Analyzer is the most aobvious important.
The analytical plan that current five classification cellanalyzers use has:
Scheme one: see Fig. 1, instrument contains four differential counting ponds 4, basicyte counting chamber 5, blood
Lactoferrin measuring cell 6, red blood cell and platelet count pond 7, divided blood sample respectively by a point blood device after blood sampling
Enter each counting chamber and measuring cell, be subsequently adding corresponding reaction reagent and react, after having reacted, four classification
In counting chamber, sample feeding detection device 1 is counted by laser scattering method, in basicyte counting chamber
Sample is also fed into detecting device 1 and is counted by laser scattering method, and in hemoglobinometry pond, sample passes through
Detection device 2 measures, and red blood cell and sample in platelet count pond are sent into detection device 3 and passed through impedance
Method carries out red blood cell and platelet count.Owing to four differential countings count common detection device with basicyte
1, need staggering time section to count, total white blood cells is from four differential counting ponds and basicyte counting chamber
Interior sample standard deviation can obtain.In four differential counting ponds, added hemolytic agent is the four independent special hemolytic agents 22 of differential counting,
In basicyte counting chamber, added hemolytic agent is that basicyte counts independent special hemolytic agent 23, blood red egg
In white measuring cell, added hemolytic agent is the independent special hemolytic agent 24 of hemoglobinometry.
The shortcoming of the program is to have employed four counting chambers, and machine system is complicated, and cost is high, and volume is big
Take up room big, it is difficult to cellanalyzer of the type five being classified is popularized for township hospital Ji Shekang center.
Scheme two: see Fig. 2, instrument contain four differential counting ponds 12, white blood cell count(WBC) pond 13, red carefully
Born of the same parents and platelet count pond 14, add blood sample to each counting chamber respectively by point blood device and reagent dispensing apparatus
React with corresponding reagent, in four differential counting ponds sample be transported to detect device 8 pass through laser scattering method
Carrying out four differential countings, in white blood cell count(WBC) pond, sample is used for carrying out total white blood cells counting, hemoglobin is surveyed
Amount and basicyte count, and wherein hemoglobinometry measures in detection device 9, and leucocyte is total
Counting number and basicyte counting are sent into detection device 10 and are counted by impedance method.Red blood cell is little with blood
In plate counting chamber, sample input carries out red blood cell and platelet count to detection device 11 by impedance method.Four points
In class counting chamber, added hemolytic agent is the four independent special hemolytic agents 25 of differential counting, adds another in white blood cell count(WBC) pond
Total white blood cells, hemoglobinometry and basicyte can be counted by a kind of hemolytic agent 26 simultaneously.
Though the shortcoming of the program is that system complexity is medium, and volume is bigger than normal only with three counting chambers, because of
This cost is the highest, and needs to take bigger working top space, it is also difficult to blood of the type five being classified
Cytoanalyze is popularized for township hospital Ji Shekang center.Additionally basicyte is counted by impedance method, phase
For contrast and laser scattering method, accuracy is poor.
Scheme three: see Fig. 3, instrument contains white blood cell count(WBC) pond 20, red blood cell and platelet count pond
21, by point blood device with reagent dispensing apparatus is divided into blood sample to two counting chambers respectively and corresponding reagent is carried out instead
Should, in white blood cell count(WBC) pond, part sample is transported to detect device 17 and counts by laser scattering method, together
Shi Jinhang total white blood cells counting, four differential countings and basicyte counting, part in white blood cell count(WBC) pond
Sample carries out hemoglobinometry at detection device 18, and red blood cell and sample input in platelet count pond are to inspection
Survey device 19 and carry out red blood cell and platelet count.It is real that white blood cell count(WBC) pond only adds a kind of hemolytic agent 27
Existing total white blood cells counting, four differential countings, basicyte counting, hemoglobinometry.
Program system is the simplest, and volume is the most minimum, it is adaptable to township hospital Ji Shekang center is suitable for, but
Due to only add a kind of hemolytic agent to realize total white blood cells counting, four differential countings, basicyte count,
Hemoglobinometry, can only get a kind of scatter diagram by laser scattering method, four classification in this scatter diagram
Leucocyte and basicyte boundary the most clearly, basicyte can only incorporate experience into Preliminary Determination counting
As a result, therefore basicyte poor accuracy, especially special sample, its accuracy is relatively difficult to ensure card.
Therefore, prior art has yet to be improved and developed.
Utility model content
The purpose of this utility model is to provide a kind of simple and reliable haemocyte five classification analysis device, it is intended to
Solve existing system complexity, volume is big, cost is high, and the problem of the poor accuracy of basicyte.
The technical solution of the utility model is as follows:
A kind of simple and reliable haemocyte five classification analysis device, it include sampling needle, white blood cell count(WBC) pond,
Dilution, the first hemolytic agent, the second hemolytic agent, five classification and Detection devices, tapping equipment and conveyor module,
Described sampling needle connects conveyor module, has coordinated sample to sample and distribution, described dilution by conveyor module
Liquid connects white blood cell count(WBC) pond and conveyor module, and described first hemolytic agent connects white blood cell count(WBC) pond and conveying mould
Block, described second hemolytic agent connects white blood cell count(WBC) pond and conveyor module, and described five classification and Detection devices connect
Conveyor module, described tapping equipment connects white blood cell count(WBC) pond.
Described device, wherein, also includes hemoglobin detection device, and described hemoglobin detection device is even
Connect white blood cell count(WBC) pond.
Described device, wherein, also includes red blood cell and platelet count pond and red blood cell detection device, institute
State red blood cell and be connected red blood cell detection device, dilution and tapping equipment with platelet count pond.
Described device, wherein, described conveyor module is distributed by syringe or constant displacement pump or carries corresponding
Sample.
Described device, wherein, described conveyor module carries out group by different number of syringe and constant displacement pump
The mode closed realizes.
The beneficial effects of the utility model: the utility model, by using leucocyte Double channel scheme, has individually
Leucocyte is processed by hemolytic agent, basophil difference can widen, better assures that basophilic
The count results of property leucocyte；Leucocyte four classify hemolytic agent that passage uses and basophil classification logical
The hemolytic agent that road uses the most not cross-mixing, independent reaction, each point will not be had influence on because mixing intersects
The classification stability of class passage；Leucocyte four classify passage, basophil classification passage share one white
Cell count pond, and common detection device, simultaneously counting chamber used by hemoglobinometry still with upper two passages
Sharing same counting chamber, whole system is simple and reliable, low cost, and volume is little, and the standard of basicyte
Really property can reach high-end five classification cellanalyzer levels, is more suitable for basic hospital and is used.
Accompanying drawing explanation
Fig. 1 is existing haemocyte five classification analysis system block diagram；
Fig. 2 is existing haemocyte five classification analysis system block diagram；
Fig. 3 is existing haemocyte five classification analysis system block diagram；
Fig. 4 is the haemocyte five classification analysis system block diagram that the utility model provides；
Fig. 5 is the haemocyte five classifying and analyzing method flow chart that the utility model provides；
Fig. 6 is the haemocyte five classification analysis apparatus structure schematic diagram that the utility model provides.
Detailed description of the invention
For making the purpose of this utility model, technical scheme and advantage clearer, clear and definite, referring to the drawings
The utility model is further described by the embodiment that develops simultaneously.
See Fig. 4, system of the present utility model contain white blood cell count(WBC) pond, red blood cell and platelet count pond,
Five classification and Detection devices, hemoglobin detection device, red blood cell detection device, by point blood device (in figure
Not shown) it is divided into blood sample and takes an entrance examination mutually in two counting chambers with reagent dispensing apparatus (not shown) respectively
Agent is reacted.This system is to be provided with two kinds of hemolytic agents with the difference of existing system, and respectively first is molten
Blood agent and the second hemolytic agent, and leucocyte four differential counting and basicyte counting are respectively by five classification
Detection device is carried out at twice；Total white blood cells counting can be with four differential countings or basicyte count synchronization.
Mixed with blood sample by the first hemolytic agent and realize leucocyte four differential counting and total white blood cells counts；Logical
The blood sample crossing the second hemolytic agent and be added with dilution mix realize basicyte counting；Use directly
The blood sample of mixed diluting liquid realizes red blood cell and platelet count by red blood cell detection device；In basophilic
Use hemoglobin detection device that the sample in white blood cell count(WBC) pond is carried out blood red egg while sexual cell counting
White measurement.
Seeing Fig. 5, the flow process of the method that the utility model provides is as follows:
S1: draw sample from machine by a point blood device；
S2: emptying white blood cell count(WBC) pond, adds the first haemolysis by reagent dispensing apparatus toward white blood cell count(WBC) pond
Agent；
S3: by point blood device, the part sample of absorption is added white blood cell count(WBC) pond and carry out with the first hemolytic agent
Reaction；
S4: reacted sample mixed liquor is sent into five classification and Detection devices by conveying device and carries out leucocyte
Four differential countings, can count total white blood cells simultaneously；
S5: emptying white blood cell count(WBC) pond again, is then carried out white blood cell count(WBC) pond by cleaning fluid；
S6: add dilution toward white blood cell count(WBC) pond by reagent dispensing apparatus, the most again will by point blood device
The part sample drawn adds white blood cell count(WBC) pond, forms mixed liquor；
S7: draw part mixed liquor from white blood cell count(WBC) pond by point blood device, then mixed liquor is added red carefully
Born of the same parents and platelet count pond；
S8: after having drawn part mixed liquor, adds second by reagent dispensing apparatus toward white blood cell count(WBC) pond molten
Blood agent is reacted；Reacted solution is sent into hemoglobin detection device and five classification by conveying device
Detection device；
S9: carry out three countings parallel: carry out red blood cell and platelet count by red blood cell detection device, lead to
Cross hemoglobin detection device and carry out hemoglobinometry in white blood cell count(WBC) pond and by five classification and Detection devices
Carry out basicyte counting, total white blood cells can be counted simultaneously；
S10: finally all results are calculated, and export each blood count result.
Wherein, if only completing five classification and Detection, method step is as follows:
S1: draw sample from machine by a point blood device；
S2: emptying white blood cell count(WBC) pond, adds the first haemolysis by reagent dispensing apparatus toward white blood cell count(WBC) pond
Agent；
S3: by point blood device, the part sample of absorption is added white blood cell count(WBC) pond and carry out with the first hemolytic agent
Reaction；
S4: reacted sample mixed liquor is sent into five classification and Detection devices by conveying device and carries out leucocyte
Four differential countings；
S5: emptying white blood cell count(WBC) pond again, is then carried out white blood cell count(WBC) pond by cleaning fluid；
S6: add dilution toward white blood cell count(WBC) pond by reagent dispensing apparatus, the most again will by point blood device
The part sample drawn adds white blood cell count(WBC) pond, forms mixed liquor；
S8: after having drawn part mixed liquor, adds second by reagent dispensing apparatus toward white blood cell count(WBC) pond molten
Blood agent is reacted；By reacted solution by conveying device send into five classification and Detection devices can carry out addicted to
Alkaline cell counts, and completes five classification and Detection.
Further:
Total white blood cells can be counted while carrying out four differential countings in step s 4；Also can be in step s 8
While carrying out basicyte counting, total white blood cells is counted.
After carrying out step S6, can will carry out step S7 flow process, it may be assumed that by point blood device from white blood cell count(WBC)
Part mixed liquor is drawn in pond, then mixed liquor adds red blood cell and platelet count pond, is examined by red blood cell
Survey device and carry out red blood cell and platelet count.
Only reacted solution in step S8 need to be added hemoglobin detection device when needing to measure hemoglobin
In.
By point blood device by after the part sample drawn and the first hemolytic agent hybrid reaction, red blood cell is dissolved,
Lymphocyte in leucocyte, monocyte, neutrophil leucocyte, eosinophil have obvious difference
Feature, by laser scattering method, can classify leucocyte four, and leucocyte also can carry out sum meter simultaneously
Number.
React after white blood cell count(WBC) pond adds dilution, part blood sample and the second hemolytic agent, reaction
After, red blood cell is dissolved, and leucocyte medium size lymphocyte, monocyte, neutrophil leucocyte, acidophil granules are thin
Born of the same parents are processed into same feature, and basicyte and this four classes leucocyte have an obvious difference characteristic and energy
Accurately make a distinction counting, therefore basicyte can be carried out accurate counting by laser scattering method, with
Time also leucocyte can be carried out total counting number.
Add hemolytic agent reaction treatment by twice, carry out leucocyte four differential counting and basicyte meter respectively
Number, thus exactly to leucocyte five differential counting.
As shown in Figure 6, the utility model provides a kind of preferably device to implement said method, and one simply may be used
Haemocyte five sorter leaned on includes sampling needle 41, red blood cell detection device 42, red blood cell and blood platelet meter
Number ponds 43, hemoglobin detection device 44, white blood cell count(WBC) pond 45, dilution the 46, first hemolytic agent 47,
Second hemolytic agent 48, five classification and Detection device 49, tapping equipment 50 and conveyor module 51.
Described sampling needle 41 is used for taking sample and distribution sample, connects conveyor module 51, passes through conveyor module
51 have coordinated sample sampling and distribution.
Described red blood cell detection device 42 connects red blood cell and platelet count pond 43, is used for red blood cell and blood
Platelet counts.Described red blood cell is used for providing red blood cell and blood platelet and dilution with platelet count pond 43
The place of liquid mixing.
Described hemoglobin detection device 44 connects white blood cell count(WBC) pond 45, is used for measuring hemoglobin.Described
White blood cell count(WBC) pond 45 is used for providing leucocyte four classification reaction, basophil classification to react and blood red
Reaction tank needed for protein measurement.
Described dilution 46 connects white blood cell count(WBC) pond 45, red blood cell and platelet count pond 43 and conveying mould
Block 51, for diluted sample and washer parts.
Described first hemolytic agent 47 connects white blood cell count(WBC) pond 45 and conveyor module 51, for lysed erythrocyte,
Lymphocyte in leucocyte, monocyte, neutrophil leucocyte, eosinophil four class cell are pulled open
Difference and be conveniently used for four differential countings.
Described second hemolytic agent 48 connects white blood cell count(WBC) pond 45 and conveyor module 51, for lysed erythrocyte
(new blend of generation can be utilized for hemoglobinometry), carries out spy by the basicyte in leucocyte
Different process, other four classes leucocytes and basophil and notable difference, thus realize basophil
Differential counting.
Described five classification and Detection devices 49 connect conveyor module 51, are to be carried out leucocyte by laser scattering method
The device of classification (including that leucocyte four classification and basophil are classified).
Described tapping equipment 50 connects white blood cell count(WBC) pond 45 and red blood cell and platelet count pond 43, is used for
The waste liquid that discharge counting process produces；Described conveyor module 51 be used for drawing sample, distribute sample, distribute dilute
Release liquid, distribution hemolytic agent, conveying detection sample enter five classification and Detection devices 49 and detect.
Wherein, described conveyor module 51 can be distributed by syringe or constant displacement pump or carry respective sample, also
Can realize by the way of different number of syringe and constant displacement pump are combined.
The detection process of this device is as follows:
1) quantitative sample is drawn by conveyor module 50 and sampling needle 41；
2) by tapping equipment 50, liquid in white blood cell count(WBC) pond 45 is drained, pass through conveyor module the most again
Hemolytic agent 47 is added white blood cell count(WBC) pond 50 by 51；
3) sample is divided to enter white blood cell count(WBC) pond 45 and first by conveyor module 51 and sampling needle 41 branch
Hemolytic agent 47 carries out hybrid reaction；
4), after reaction certain time, by conveyor module 51, mixed liquor is delivered to detect device 49 and carries out white
Cell four differential counting, thus lymphocyte, monocyte, neutrophil leucocyte, eosinophil are entered
Row accurate counting；
5) by tapping equipment 50, remaining mixed liquor in white blood cell count(WBC) pond 45 is emptied；
6) by conveyor module 51, dilution 46 is added white blood cell count(WBC) pond 45, the most again by conveying mould
Sample is divided into white blood cell count(WBC) pond and mixes by block 51 and sampling needle 41；
7) mixed from white blood cell count(WBC) pond 45 extraction part by conveyor module 51 and sampling needle 41 after having mixed
Close sample, then this part mixing sample is divided into red blood cell and platelet count pond 43；
9) react toward addition the second hemolytic agent 48 in remaining mixing sample in white blood cell count(WBC) pond 45；
10), after reaction, hemoglobinometry is carried out by detection device 44, and will by conveyor module 51
Reacted mixing sample is delivered to detect device 49 and carries out basophil differential counting；Meanwhile lead to
Cross detection device 42 red blood cell and blood platelet are counted；
11) complete all countings, export result.
Total white blood cells counting can pass through step 4 or step 10, and it is total that these two steps all can obtain leucocyte
Number result.
The utility model, by using leucocyte Double channel scheme, has independent hemolytic agent to process leucocyte,
Basophil difference can widen, better assure that the count results of basophil；White thin
Born of the same parents four classify passage use hemolytic agent and basophil classification passage use hemolytic agent do not hand over
Fork mixing, independent reaction, the classification stability of each classification passage will not be had influence on because mixing intersects；White thin
Classify passage, basophil classification passage of born of the same parents four shares a white blood cell count(WBC) pond, and shares detection
Device, counting chamber used by hemoglobinometry still shares same counting chamber, whole system with upper two passages simultaneously
Simple and reliable, low cost, volume is little, and the accuracy of basicyte can reach high-end five classification blood
Cytoanalyze level, is more suitable for basic hospital and is used.
It should be appreciated that application of the present utility model is not limited to above-mentioned citing, to ordinary skill
For personnel, can be improved according to the above description or convert, all these modifications and variations all should belong to
The protection domain of the utility model claims.
Claims (5)
1. a simple and reliable haemocyte five classification analysis device, it is characterised in that include sampling needle, white
Cell count pond, dilution, the first hemolytic agent, the second hemolytic agent, five classification and Detection devices, tapping equipment
And conveyor module, described sampling needle connects conveyor module, has coordinated sample to sample by conveyor module and has divided
Joining, described dilution connects white blood cell count(WBC) pond and conveyor module, and described first hemolytic agent connects leucocyte meter
Number pond and conveyor module, described second hemolytic agent connects white blood cell count(WBC) pond and conveyor module, described five classification
Detection device connects conveyor module, and described tapping equipment connects white blood cell count(WBC) pond.
Device the most according to claim 1, it is characterised in that also include hemoglobin detection device,
Described hemoglobin detection device connects white blood cell count(WBC) pond.
Device the most according to claim 1, it is characterised in that also include red blood cell and platelet count
Pond and red blood cell detection device, described red blood cell is connected red blood cell detection device, dilution with platelet count pond
Liquid and tapping equipment.
Device the most according to claim 1, it is characterised in that described conveyor module by syringe or
Constant displacement pump distributes or carries respective sample.
Device the most according to claim 1, it is characterised in that described conveyor module is by different numbers
Syringe and the mode that is combined of constant displacement pump realize.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620085594.5U CN205538976U (en) | 2016-01-28 | 2016-01-28 | Simple reliable five categorised analytical equipment of blood cell |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620085594.5U CN205538976U (en) | 2016-01-28 | 2016-01-28 | Simple reliable five categorised analytical equipment of blood cell |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108303363A (en) * | 2017-01-12 | 2018-07-20 | 长春市布拉泽医疗科技有限公司 | A kind of blood cell analysis method and the cellanalyzer using this method |
| CN109270281A (en) * | 2017-07-18 | 2019-01-25 | 深圳市帝迈生物技术有限公司 | Improve the method and apparatus of leukocyte differential count result accuracy and count results repeatability |
| CN111849736A (en) * | 2019-04-25 | 2020-10-30 | 深圳市帝迈生物技术有限公司 | Animal blood cell measuring method and animal blood analyzing apparatus |
| CN113008653A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Diluent, hemocyte analyzer, reagent for hemocyte analyzer, and kit |
| CN113777332A (en) * | 2020-06-09 | 2021-12-10 | 深圳迈瑞生物医疗电子股份有限公司 | Immunoassay instrument and autoimmune analysis method |
-
2016
- 2016-01-28 CN CN201620085594.5U patent/CN205538976U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108303363A (en) * | 2017-01-12 | 2018-07-20 | 长春市布拉泽医疗科技有限公司 | A kind of blood cell analysis method and the cellanalyzer using this method |
| CN108303363B (en) * | 2017-01-12 | 2023-08-22 | 长春市布拉泽医疗科技有限公司 | Blood cell analysis method and blood cell analyzer using same |
| CN109270281A (en) * | 2017-07-18 | 2019-01-25 | 深圳市帝迈生物技术有限公司 | Improve the method and apparatus of leukocyte differential count result accuracy and count results repeatability |
| CN111849736A (en) * | 2019-04-25 | 2020-10-30 | 深圳市帝迈生物技术有限公司 | Animal blood cell measuring method and animal blood analyzing apparatus |
| CN113008653A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Diluent, hemocyte analyzer, reagent for hemocyte analyzer, and kit |
| CN113777332A (en) * | 2020-06-09 | 2021-12-10 | 深圳迈瑞生物医疗电子股份有限公司 | Immunoassay instrument and autoimmune analysis method |
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