CN111239027A - Blood particle detection method and blood analyzer - Google Patents
Blood particle detection method and blood analyzer Download PDFInfo
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Abstract
The embodiment of the invention discloses a blood particle detection method and a blood analyzer thereof. The method comprises the following steps: determining whether the collected blood sample belongs to a normal sample or a low-value sample; when the blood sample belongs to a low-value sample, reacting the blood sample with a corresponding reagent to form a first low-value sample solution and a second low-value sample solution; when the blood sample belongs to a normal sample, reacting the blood sample with a corresponding reagent to form a first normal sample solution and a second normal sample solution; detecting leukocytes of said blood sample by said first low value sample fluid or first normal sample fluid, respectively, and erythrocytes and platelets of said blood sample by said second low value sample fluid or said second normal sample fluid, respectively, using electrical impedance methods. The method can well overcome the defects of the existing blood detection system. The detection device does not need to be additionally provided with an optical system and use a sectional type detection mode, can improve the detection accuracy of low-value samples and simplifies the structure of the detector.
Description
Technical Field
The invention relates to the technical field of blood analysis, in particular to a blood particle detection method and a blood analyzer thereof.
Background
In clinical medicine, a biological sample such as blood is usually collected from a human body and used for detecting the distribution of a part of cell particles (such as red blood cells, white blood cells, platelets, and the like) in the human body so as to help a doctor diagnose the health condition of the human body.
Among them, leukocytes and platelets have important reference values in clinical medicine, and therefore, the detection accuracy of leukocytes and platelets is highly required. Existing blood particle detection is typically implemented in conjunction with automated or semi-automated blood analyzers or related blood analysis systems.
In the conventional blood cell detecting system or blood analyzer, the detection of the blood particle components is mainly accomplished by using an optical system and an electrical impedance system in combination. Among them, the optical system is mainly used for detecting leukocytes or platelets in blood samples. While electrical impedance systems are used to detect platelets and the like. This allows the detection of a specific component in blood, but many of these devices are bulky and costly.
In the process of implementing the present application, the inventor finds that the following problems exist in the prior art: when the existing blood detection instrument detects white blood cells, platelets and the like, an optical system is needed to realize the detection. The optical system has high implementation cost, needs to perform leukocyte detection and platelet detection in a segmented manner, has high requirements on an instrument system, and is not beneficial to reducing the manufacturing cost and the volume of instrument equipment.
Disclosure of Invention
The embodiment of the invention provides a blood particle detection method and a blood analyzer thereof, which are used for solving the problems of higher cost and larger volume of an optical system adopted by the conventional blood analyzer.
A first aspect of an embodiment of the present invention provides a blood particle detection method. Wherein the method comprises the following steps:
determining whether the collected blood sample belongs to a normal sample or a low-value sample;
when the blood sample belongs to a low-value sample, reacting the blood sample with a corresponding reagent to form a first low-value sample solution and a second low-value sample solution;
when the blood sample belongs to a normal sample, reacting the blood sample with a corresponding reagent to form a first normal sample solution and a second normal sample solution;
detecting leukocytes of said blood sample by said first low value sample fluid or first normal sample fluid, respectively, and erythrocytes and platelets of said blood sample by said second low value sample fluid or said second normal sample fluid, respectively, using electrical impedance methods.
Optionally, reacting the blood sample with a corresponding reagent to form a first low-value sample solution and a second low-value sample solution, specifically including:
collecting blood samples and diluting the blood samples in a first counting cell;
moving the diluted blood sample removal portion into a second counting well;
and adding corresponding reagents into the first counting cell and the second counting cell for reaction to respectively form a first low-value sample liquid and a second low-value sample liquid.
Optionally, when the blood sample belongs to a low value sample, detecting white blood cells of the blood sample through the first low value sample solution and detecting red blood cell and platelet counts of the blood sample through the second low value sample solution by using an electrical impedance method, specifically including:
establishing a preset first negative pressure through a negative pressure injector;
leading the first low-value sample liquid and the second low-value sample liquid to flow through a first impedance detection area and a second impedance detection area respectively at a first speed at a set first time by the first negative pressure;
collecting a first electrical signal generated when the first low-value sample liquid flows through the first impedance detection area and a second electrical signal generated when the second low-value sample liquid flows through the second impedance detection area during the first time;
statistically analyzing said first electrical signal to obtain a white blood cell count of said blood sample;
and is
Statistically analyzing the second electrical signal to obtain a red blood cell and platelet count of the blood sample.
Optionally, when the blood sample belongs to a normal sample, detecting white blood cells of the blood sample through the first normal sample fluid and detecting red blood cell and platelet counts of the blood sample through the second normal sample fluid by using an electrical impedance method, specifically including:
establishing a preset second negative pressure through the negative pressure injector;
leading the first normal sample liquid and the second normal sample liquid to flow through the first impedance detection area and the second impedance detection area respectively at a second speed at a set second time by the second negative pressure;
collecting a first electric signal generated when the first normal sample liquid flows through the first impedance detection area and a second electric signal generated when the second normal sample liquid flows through the second impedance detection area in the second time;
statistically analyzing said first electrical signal to obtain a white blood cell count of said blood sample;
and is
Statistically analyzing the second electrical signal to obtain a red blood cell and platelet count of the blood sample.
Optionally, the first time is longer than the second time so that the blood sample belonging to the low value sample is counted more in the impedance detection zone than the blood sample belonging to the normal sample.
Optionally, determining whether the collected blood sample belongs to a normal sample or a low-value sample specifically includes:
under the condition that the blood sample is defaulted to be a normal sample, carrying out particle detection on the blood sample;
judging whether the particle detection result is lower than a normal range;
if so, determining that the collected blood sample belongs to a low-value sample;
if not, determining that the blood sample belongs to a normal sample.
Optionally, the method further comprises:
detecting the hemoglobin concentration of said blood sample by said first low value sample fluid or said first normal sample fluid using a colorimetric method.
A second aspect of embodiments of the present invention provides a blood analyzer. Wherein the blood analyzer comprises:
the sample preparation mechanism is used for collecting blood samples and obtaining corresponding reagents;
the reaction mechanism is used for providing reaction sites of the blood sample and the corresponding reagent so as to form corresponding first low-value sample liquid and second low-value sample liquid or first normal sample liquid and second normal sample liquid;
the detection mechanism comprises a first impedance detection area and a second impedance detection area, and is used for detecting the sample liquid flowing through the first impedance detection area and the second impedance detection area respectively by an electrical impedance method;
the mode setting mechanism is used for setting a corresponding measurement mode according to whether the current blood sample belongs to a normal sample or a low-value sample;
the information processing mechanism is used for receiving and processing the electric signal detected and obtained by the detection mechanism;
and the control mechanism is used for controlling the sample preparation mechanism, the detection mechanism, the information processing mechanism and the mode setting mechanism.
Optionally, the sample preparation mechanism comprises a horizontal movement motor, a vertical movement motor, a liquid transfer needle and an injector;
the horizontal movement motor and the vertical movement motor are driving mechanisms and are used for driving the liquid-transferring needle and the injector to move along corresponding directions;
the pipetting needle and the syringe are used for collecting blood samples and corresponding reagents.
Optionally, the detection mechanism comprises: the device comprises a negative pressure pump, a first counting pool, a second counting pool and a counting liquid path device;
the negative pressure pump is used for establishing negative pressure to guide the first low-value sample liquid and the second low-value sample liquid to respectively flow through the first counting pool and the second counting pool at set time and speed;
the counting liquid path device is used for collecting electric signals generated when the counting liquid flows through the first counting cell and the second counting cell; wherein the electrical signal generated by the first counting cell is used for detecting the white blood cells of the blood sample, and the electrical signal generated by the second counting cell is used for detecting the red blood cell and platelet number of the blood sample; when the current blood sample belongs to a low-value sample, the time for flowing through the first counting cell and the second counting cell is longer than that when the current sample belongs to a normal sample, the time for flowing through the first counting cell and the second counting cell is longer;
the detection mechanism further comprises a colorimetric detection device; the colorimetric detection device is used for detecting the hemoglobin concentration of the first low-value sample liquid or the first normal sample liquid in the first counting cell by using a colorimetric method.
The blood particle detection method and the blood analyzer thereof provided by the embodiment of the invention can well overcome the defects of the existing blood detection system by setting a plurality of detection modes and a plurality of impedance detection areas. The method does not need to add an optical system and use a sectional type detection mode, can well simplify the requirements of a blood detection system, can improve the detection accuracy of low-value samples, and has good application prospect.
The foregoing description is only an overview of the technical solutions of the embodiments of the present invention, and the embodiments of the present invention can be implemented according to the content of the description in order to make the technical means of the embodiments of the present invention more clearly understood, and the detailed description of the present invention is provided below in order to make the foregoing and other objects, features, and advantages of the embodiments of the present invention more clearly understandable.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic diagram of a blood particle detection method according to an embodiment of the present invention;
FIG. 2 is a block diagram of a blood analyzer according to an embodiment of the present invention;
FIG. 3a is a schematic diagram of a detection process for low-value samples according to an embodiment of the present invention;
FIG. 3b is a schematic diagram of a detection process of a normal sample according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It will be understood that the terms "comprises" and/or "comprising," when used in this specification and the appended claims, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.
Fig. 1 shows a flow chart of a blood particle detection method provided by an embodiment of the invention. The blood particle detection method can realize the simultaneous detection of white blood cells and platelets in a blood sample, and has good detection accuracy for low-value samples. As shown in fig. 1, the method may include the steps of:
step 110: it is determined whether the collected blood sample is a normal sample or a low value sample. If the low value sample belongs to, step 120 is executed, and if the normal value sample belongs to, step 130 is executed.
"Low value sample" and "Normal sample" are concepts of relativity. A low value sample is a blood sample that has a far lower blood cell or platelet count than a normal value range relative to a normal sample. Which may be present in various possible types of patients.
The range of counts for blood samples from low value samples is very different from that of normal samples. Therefore, it is necessary to perform a targeted process so as not to cause a problem of lowering the detection accuracy.
In some embodiments, the type of blood sample specifically collected may be determined by:
first, a particle test is performed on the blood sample in a case where the blood sample is a normal sample by default. Before the determination, the blood sample is detected in a default mode (normal sample) in advance, and the approximate blood component condition can be obtained.
Then, it is judged whether the particle detection result is lower than a normal range. If yes, the collected blood sample is determined to belong to a low value sample. If not, determining that the blood sample belongs to a normal sample. Blood samples belonging to low-value samples can be identified by using special identification so as to be convenient for subsequent detection by adopting a correct detection mode.
The normal range is a preset empirical value, and can be set by comprehensively considering various factors such as actual conditions.
Step 120: and reacting the blood sample with a corresponding reagent to form a first low-value sample solution and a second low-value sample solution.
The collected blood sample is subjected to a number of pretreatment steps before being subjected to a component count analysis, and is reacted with a diluent, a hemolytic agent, and the like to obtain a sample solution that can be tested. The specific selection of the type, concentration, reaction time, reaction mode and the like of the reagents can be selected or set by the skilled person according to the needs of the actual situation, which is well known by the person skilled in the art and will not be described herein.
In this embodiment, after the same blood sample is processed, the blood sample is divided into a first low value sample solution and a second low value sample solution for detecting and counting different blood components.
Specifically, a certain amount of blood may be collected from the blood sample by a lancet or the like, added to the first counting chamber, and diluted. The blood sample diluted in the first counting chamber can be partially taken out and transferred to the second counting chamber by a blood separating needle or the like. And finally, adding corresponding reagents into the blood samples diluted in the first counting cell and the second counting cell, reacting and uniformly mixing to form the first low-value sample liquid and the second low-value sample liquid.
Step 130: and reacting the blood sample with a corresponding reagent to form a first normal sample solution and a second normal sample solution.
When the blood sample is determined to be a normal sample, the required sample liquid is obtained by using a reaction mode corresponding to the normal sample. In the present embodiment, in order to distinguish from a sample liquid formed by a blood sample belonging to a low-value sample, a first normal sample liquid and a second normal sample liquid are used for designation.
It should be noted that the apparatus or mechanism required for forming the first normal sample solution and the second normal sample solution is the same as that in step 120, and the difference between them is only the ratio of the added blood sample, the reagent, and the dilution.
Step 140: detecting leukocytes of said blood sample by said first low value sample fluid and detecting red blood cell and platelet counts of said blood sample by said second low value sample fluid using electrical impedance.
Electrical impedance is a commonly used cell technology method. This is achieved by the principle of electrical signal pulses generated by cells suspended in an electrolyte solution as they pass through a counting zone.
In this embodiment, based on the two independent sample solutions formed in step 130, leukocytes and platelets of the blood sample can be detected in different impedance detection regions, respectively.
Step 150: detecting leukocytes of said blood sample by a first normal sample fluid and detecting red blood cell and platelet counts of said blood sample by said second normal sample fluid using electrical impedance.
For blood samples belonging to normal samples, electrical impedance methods are also used to achieve white blood cell and platelet counts. Due to the difference in the number of detections between the normal and low value samples. Therefore, the specific parameters of the electrical impedance method used in steps 140 and 150 need to be adjusted accordingly.
According to the blood particle detection method provided by the embodiment of the invention, the special detection mode of the low-value sample is additionally arranged, and when the blood sample is determined to be the low-value sample, the detection process can be adaptively adjusted, so that the detection accuracy of the low-value sample is effectively improved.
In addition, the first sample liquid and the second sample liquid are respectively split for detection during detection, an optical system is not needed, the simultaneous detection of white blood cells and platelets can be realized, a hardware system is effectively simplified, and the equipment cost is reduced.
Step 160: the hemoglobin concentration of the first sample liquid (i.e., the first low-value sample liquid or the first normal sample liquid) is detected by colorimetry.
The first sample solution can be used for detecting the hemoglobin concentration of a blood sample, in addition to the white blood cell detection. In this embodiment, the hemoglobin concentration can be detected continuously with the electrical impedance method. That is, the hemoglobin concentration and the white blood cells of the first sample solution are sequentially detected.
In some embodiments, the specific steps of the electrical impedance method for detecting blood particles include:
when the blood sample belongs to a low-value sample, a preset first negative pressure is firstly established through the negative pressure injector. Then, the first low-value sample liquid and the second low-value sample liquid are guided by the first negative pressure to flow through the first impedance detection area and the second impedance detection area respectively at a first speed at a set first time so as to enable the corresponding detection circuit to collect the first time, wherein the first low-value sample liquid flows through a first electrical signal generated when the first low-value sample liquid flows through the first impedance detection area and the second low-value sample liquid flows through a second electrical signal generated when the second low-value sample liquid flows through the second impedance detection area.
Finally, the first electrical signal is statistically analyzed to obtain a white blood cell count of the blood sample, and the second electrical signal is statistically analyzed to obtain a red blood cell and platelet count of the blood sample.
In particular, any suitable type of statistical analysis may be used. Which are well known to those skilled in the art and will not be described in detail herein.
When the blood sample belongs to a normal sample, a preset second negative pressure is firstly established through the negative pressure injector. Then, the first normal sample liquid and the second normal sample liquid are guided by the second negative pressure to flow through the first impedance detection area and the second impedance detection area respectively at a second speed at a set second time so that the corresponding detection circuit collects a first electric signal generated when the first normal sample liquid flows through the first impedance detection area and a second electric signal generated when the second normal sample liquid flows through the second impedance detection area during the second time.
Finally, the first electrical signal is statistically analyzed to obtain a white blood cell count of the blood sample, and the second electrical signal is statistically analyzed to obtain a red blood cell and platelet count of the blood sample.
Based on the difference between the data ranges of the low-value sample and the normal sample, one or more parameters involved in the electrical impedance method detection process can be adjusted correspondingly so as to enable the low-value sample to have better detection accuracy.
In some embodiments, the first time may be set longer than the second time to allow the blood sample belonging to the low value sample to count more at the impedance detection zone than the blood sample belonging to the normal sample, thereby ensuring that the number of cellular particles in the low value sample is not underestimated.
In other embodiments, the same effect can be obtained by adjusting the flow speed of the sample solution (i.e. adjusting the first speed and the second speed) or the concentration of the cell particles in the sample solution (i.e. adjusting the dilution of the blood sample with a reaction reagent such as a diluent or a hemolytic agent), so that the blood sample belonging to the low value sample is counted more in the impedance detection area than the blood sample belonging to the normal sample, and it is ensured that the number of the cell particles in the low value sample is not underestimated.
Fig. 2 is a block diagram of a blood analyzer according to an embodiment of the present invention. By applying the blood particle detection method provided by the embodiment of the invention, an optical system and sectional detection are not needed, so that the cost of instrument equipment can be effectively reduced and the instrument can be simplified. As shown in fig. 2, the blood analyzer includes: a sample preparation means 210, a reaction means 220, a detection means 230, a mode setting means 240, an information processing means 250, and a control means 260.
The sample preparation mechanism 210 is a collection device for collecting blood samples and obtaining corresponding reagents. It may specifically include a horizontal motion motor, a vertical motion motor, a pipetting needle, a syringe, and the like. In practical use, the horizontal movement motor and the vertical movement motor are used as driving mechanisms and used for driving the liquid transferring needle and the syringe to move along corresponding directions so as to finish the collection of blood samples and the acquisition of reagents.
The specific arrangement of the sample preparation mechanism can be adjusted or added or deleted according to the needs of actual conditions, which is well known to those skilled in the art.
The reaction mechanism 220 is a device for providing a reaction site between a blood sample and a corresponding reagent. The reaction mechanism 220 may provide at least two independent places for mixing the blood sample with the diluent and the hemolytic agent and performing a reaction, etc. to form the corresponding first sample solution and second sample solution.
When the sample is a low value sample, the first sample liquid and the second sample liquid may be referred to as a first low value sample liquid and a second low value sample liquid, respectively. When the sample is a normal sample, the first sample liquid and the second sample liquid may be referred to as a first normal sample and a second normal sample liquid, respectively.
The detecting unit 230 is a device for performing cell counting by an electrical impedance method. In order to meet the requirement of simultaneous detection of leukocytes and platelets, the detection mechanism 230 may have first and second impedance detection regions for detecting the sample liquid flowing through the first and second impedance detection regions, respectively.
Specifically, the detection mechanism 230 may include a negative pressure pump, a first counting cell, a second counting cell, and a counting solution path device.
Establishing negative pressure by the negative pressure pump to guide the first sample liquid and the second sample liquid to flow through the first counting cell and the second counting cell respectively at set time and speed. And pulse signals generated when the liquid flows through the first counting cell and the second counting cell are acquired by the counting liquid path device.
In particular, the electrical signal generated by the flow through the first counting cell can be used to detect the white blood cells of the blood sample. The electrical signal generated by the flow through the second cell is then used to detect the red blood cell and platelet count of the blood sample.
Preferably, the detection mechanism 230 may further comprise a colorimetric detection device for detecting the hemoglobin concentration of the blood sample. The colorimetric detection device can detect the first low-value sample liquid or the first normal sample liquid in the first counting cell by using a colorimetric method so as to obtain the hemoglobin concentration of a blood sample.
The mode setting mechanism 240 is a device for setting a corresponding measurement mode according to whether the current blood sample is a normal sample or a low-value sample. The mode setting mechanism 240 may be a manual or automatic setting that allows the blood analyzer to operate in the correct manner.
The information processing means 250 is a functional module for receiving and processing the electric signal detected by the detecting means. The method can be realized by combining software and hardware, executes a proper statistical analysis algorithm to realize the processing of the electric signals, and outputs corresponding data information to be provided for the reference of medical staff.
The control mechanism 260 is the control core of the entire blood analyzer. The system can be used for controlling the sample preparation mechanism, the detection mechanism, the information processing mechanism and the mode setting mechanism and coordinating the operation among all the functional modules. The control mechanism 260 may be implemented by any suitable processor or processing chip, and may be adjusted and configured by those skilled in the art according to the actual requirements, in combination with corresponding computer program instructions.
The blood analyzer provided by the embodiment of the invention can simplify the structure of the detection system by applying the blood detection method provided by the embodiment of the invention, and effectively improves the accuracy of the electrical impedance detection system in measuring the low-value sample of the white blood cells/platelets by increasing the number of the cytometers on the premise of not adopting an optical system. Moreover, the measurement of the two is carried out simultaneously without interference.
In addition, the blood analyzer has the advantages that the optical system is removed, the whole mechanical structure can be simplified, the manufacturing cost and the use cost are reduced, and the blood analyzer is beneficial to miniaturization and convenient to transport and carry.
Fig. 3a and 3b are schematic diagrams of a blood analyzer for detecting low-value samples and normal samples, respectively. As shown in fig. 3a and 3b, when the type of blood sample is determined, the blood analyzer may be set to a corresponding low value sample test mode or normal sample test mode.
In the low value sample testing mode, two blood samples (blood sample one and blood sample two) are collected for white blood cell count and platelet (and other cell) count, respectively, as shown in fig. 3 a. The two blood samples are added with corresponding reagents (such as hemolytic agent and diluent), mixed and reacted for a corresponding time T1 to form a first sample solution and a second sample solution.
The first and second sample liquids are detected in the cell 1 and the cell 2, respectively, by means of an electrical impedance detection system (flow-through times indicated as T2+ T1 and T2+ T2, respectively, having a longer flow-through time than the normal sample). That is, when detecting leukocytes and platelets, the flow-through time (t1 and t2 may have different values) can be adjusted in a targeted manner to improve the detection accuracy of different components in the blood sample.
In the normal sample testing mode, two first and second blood samples are taken for white blood cell count and platelet (and other cell) count, respectively, as shown in fig. 3 b. The first and second blood samples are filled with corresponding reagents (e.g., hemolytic agent, diluent), mixed and reacted for a corresponding time T1 to form a first and second sample solutions.
The first sample solution and the second sample solution are respectively detected in the counting cell 1 and the counting cell 2 by an electrical impedance method (the flowing time is respectively represented as T2), and the detection results of white blood cells and platelets are obtained. In addition, the first sample solution in the counting chamber 1 can be detected by colorimetry to obtain the hemoglobin concentration of the blood sample.
In the description provided herein, numerous specific details are set forth. It is understood, however, that embodiments of the invention may be practiced without these specific details. In some instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description.
Similarly, it should be appreciated that in the foregoing description of exemplary embodiments of the invention, various features of the embodiments of the invention are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the invention and aiding in the understanding of one or more of the various inventive aspects. However, the disclosed method should not be interpreted as reflecting an intention that: that the invention as claimed requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this invention.
Those skilled in the art will appreciate that the modules in the device in an embodiment may be adaptively changed and disposed in one or more devices different from the embodiment. The modules or units or components of the embodiments may be combined into one module or unit or component, and furthermore they may be divided into a plurality of sub-modules or sub-units or sub-components. All of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and all of the processes or elements of any method or apparatus so disclosed, may be combined in any combination, except combinations where at least some of such features and/or processes or elements are mutually exclusive. Each feature disclosed in this specification (including any accompanying claims, abstract and drawings) may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise.
While the invention has been described with reference to specific embodiments, the invention is not limited thereto, and various equivalent modifications and substitutions can be easily made by those skilled in the art within the technical scope of the invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A method for detecting blood particles, comprising:
determining whether the collected blood sample belongs to a normal sample or a low-value sample;
when the blood sample belongs to a low-value sample, reacting the blood sample with a corresponding reagent to form a first low-value sample solution and a second low-value sample solution;
when the blood sample belongs to a normal sample, reacting the blood sample with a corresponding reagent to form a first normal sample solution and a second normal sample solution;
detecting leukocytes of said blood sample by said first low value sample fluid or first normal sample fluid, respectively, and erythrocytes and platelets of said blood sample by said second low value sample fluid or said second normal sample fluid, respectively, using electrical impedance methods.
2. The method according to claim 1, wherein reacting the blood sample with a corresponding reagent to form a first low value sample fluid and a second low value sample fluid comprises:
collecting blood samples and diluting the blood samples in a first counting cell;
moving the diluted blood sample removal portion into a second counting well;
adding corresponding reagents into the first counting cell and the second counting cell for reaction to respectively form
The first low value sample fluid and the second low value sample fluid.
3. The method according to claim 2, wherein, when said blood sample belongs to a low value sample, detecting white blood cells of said blood sample by said first low value sample fluid and detecting red blood cell and platelet counts of said blood sample by said second low value sample fluid using electrical impedance method, in particular comprising:
establishing a preset first negative pressure through a negative pressure injector;
leading the first low-value sample liquid and the second low-value sample liquid to flow through a first impedance detection area and a second impedance detection area respectively at a first speed at a set first time by the first negative pressure;
collecting a first electrical signal generated when the first low-value sample liquid flows through the first impedance detection area and a second electrical signal generated when the second low-value sample liquid flows through the second impedance detection area during the first time;
statistically analyzing said first electrical signal to obtain a white blood cell count of said blood sample;
and is
Statistically analyzing the second electrical signal to obtain a red blood cell and platelet count of the blood sample.
4. The method according to claim 3, wherein the detecting the white blood cells of the blood sample by the first normal sample fluid and the detecting the red blood cell and platelet count of the blood sample by the second normal sample fluid by electrical impedance method when the blood sample belongs to a normal sample, comprises:
establishing a preset second negative pressure through the negative pressure injector;
leading the first normal sample liquid and the second normal sample liquid to flow through the first impedance detection area and the second impedance detection area respectively at a second speed at a set second time by the second negative pressure;
collecting a first electric signal generated when the first normal sample liquid flows through the first impedance detection area and a second electric signal generated when the second normal sample liquid flows through the second impedance detection area in the second time;
statistically analyzing said first electrical signal to obtain a white blood cell count of said blood sample;
and is
Statistically analyzing the second electrical signal to obtain a red blood cell and platelet count of the blood sample.
5. The method of claim 4, wherein said first time is longer than said second time so that said blood sample belonging to the low value sample counts more in the impedance detection zone than said blood sample belonging to the normal sample.
6. The method of claim 1, wherein determining whether the collected blood sample is a normal sample or a low value sample comprises:
under the condition that the blood sample is defaulted to be a normal sample, carrying out particle detection on the blood sample;
judging whether the particle detection result is lower than a normal range;
if so, determining that the collected blood sample belongs to a low-value sample;
if not, determining that the blood sample belongs to a normal sample.
7. The method of claim 1, further comprising:
detecting the hemoglobin concentration of said blood sample by said first low value sample fluid or said first normal sample fluid using a colorimetric method.
8. A blood analyzer, comprising:
the sample preparation mechanism is used for collecting blood samples and obtaining corresponding reagents;
the reaction mechanism is used for providing reaction sites of the blood sample and the corresponding reagent so as to form corresponding first low-value sample liquid and second low-value sample liquid or first normal sample liquid and second normal sample liquid;
the detection mechanism comprises a first impedance detection area and a second impedance detection area, and is used for detecting the sample liquid flowing through the first impedance detection area and the second impedance detection area respectively by an electrical impedance method;
the mode setting mechanism is used for setting a corresponding measurement mode according to whether the current blood sample belongs to a normal sample or a low-value sample;
the information processing mechanism is used for receiving and processing the electric signal detected and obtained by the detection mechanism;
and the control mechanism is used for controlling the sample preparation mechanism, the detection mechanism, the information processing mechanism and the mode setting mechanism.
9. The blood analyzer of claim 8, wherein the sample preparation mechanism comprises a horizontal motion motor, a vertical motion motor, a pipetting needle, and a syringe;
the horizontal movement motor and the vertical movement motor are driving mechanisms and are used for driving the liquid-transferring needle and the injector to move along corresponding directions;
the pipetting needle and the syringe are used for collecting blood samples and corresponding reagents.
10. The blood analyzer of claim 8, wherein the detection mechanism comprises: the device comprises a negative pressure pump, a first counting pool, a second counting pool and a counting liquid path device;
the negative pressure pump is used for establishing negative pressure to guide the first low-value sample liquid and the second low-value sample liquid to respectively flow through the first counting pool and the second counting pool at set time and speed;
the counting liquid path device is used for collecting electric signals generated when the counting liquid flows through the first counting cell and the second counting cell; wherein the electrical signal generated by the first counting cell is used for detecting the white blood cells of the blood sample, and the electrical signal generated by the second counting cell is used for detecting the red blood cell and platelet number of the blood sample; when the current blood sample belongs to a low-value sample, the time for flowing through the first counting cell and the second counting cell is longer than that when the current sample belongs to a normal sample, the time for flowing through the first counting cell and the second counting cell is longer;
the detection mechanism further comprises a colorimetric detection device; the colorimetric detection device is used for detecting the hemoglobin concentration of the first low-value sample liquid or the first normal sample liquid in the first counting cell by using a colorimetric method.
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| CN202010074467.6A CN111239027A (en) | 2020-01-22 | 2020-01-22 | Blood particle detection method and blood analyzer |
| PCT/CN2020/084259 WO2021147179A1 (en) | 2020-01-22 | 2020-04-10 | Blood particle detection method and blood analyzer thereof |
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| CN202010074467.6A CN111239027A (en) | 2020-01-22 | 2020-01-22 | Blood particle detection method and blood analyzer |
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| CN113777009B (en) * | 2021-08-31 | 2024-04-16 | 深圳迈瑞动物医疗科技股份有限公司 | Cell analyzer and cell detection method suitable for sheep, goats and cows |
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| WO2021147179A1 (en) | 2021-07-29 |
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