CN113777009B - Cell analyzer and cell detection method suitable for sheep, goats and cows - Google Patents
Cell analyzer and cell detection method suitable for sheep, goats and cows Download PDFInfo
- Publication number
- CN113777009B CN113777009B CN202111017316.8A CN202111017316A CN113777009B CN 113777009 B CN113777009 B CN 113777009B CN 202111017316 A CN202111017316 A CN 202111017316A CN 113777009 B CN113777009 B CN 113777009B
- Authority
- CN
- China
- Prior art keywords
- detection
- white blood
- cell
- sample liquid
- blood cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 572
- 241001494479 Pecora Species 0.000 title claims abstract description 83
- 241000283690 Bos taurus Species 0.000 title claims abstract description 80
- 241000283707 Capra Species 0.000 title claims abstract description 80
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 365
- 239000007788 liquid Substances 0.000 claims abstract description 268
- 210000004027 cell Anatomy 0.000 claims abstract description 228
- 238000004820 blood count Methods 0.000 claims abstract description 206
- 210000004369 blood Anatomy 0.000 claims abstract description 171
- 239000008280 blood Substances 0.000 claims abstract description 171
- 239000003085 diluting agent Substances 0.000 claims abstract description 94
- 241001465754 Metazoa Species 0.000 claims abstract description 81
- 238000000034 method Methods 0.000 claims abstract description 76
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 60
- 230000003993 interaction Effects 0.000 claims abstract description 44
- 238000012360 testing method Methods 0.000 claims description 121
- 210000003743 erythrocyte Anatomy 0.000 claims description 82
- 238000010790 dilution Methods 0.000 claims description 81
- 239000012895 dilution Substances 0.000 claims description 81
- 238000005070 sampling Methods 0.000 claims description 78
- 238000002360 preparation method Methods 0.000 claims description 73
- 238000007865 diluting Methods 0.000 claims description 35
- 239000012634 fragment Substances 0.000 claims description 32
- 206010018910 Haemolysis Diseases 0.000 claims description 25
- 230000008588 hemolysis Effects 0.000 claims description 25
- 239000003795 chemical substances by application Substances 0.000 claims description 22
- 241000282326 Felis catus Species 0.000 claims description 18
- 230000009471 action Effects 0.000 claims description 17
- 239000012530 fluid Substances 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 14
- 241000283073 Equus caballus Species 0.000 claims description 11
- 101100377801 Arabidopsis thaliana ABCG1 gene Proteins 0.000 claims description 8
- 101100433753 Arabidopsis thaliana ABCG2 gene Proteins 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 238000007405 data analysis Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 349
- 239000012488 sample solution Substances 0.000 description 27
- 241000282472 Canis lupus familiaris Species 0.000 description 18
- 238000002156 mixing Methods 0.000 description 17
- 230000003287 optical effect Effects 0.000 description 14
- 210000004698 lymphocyte Anatomy 0.000 description 13
- 241000283086 Equidae Species 0.000 description 11
- 230000009286 beneficial effect Effects 0.000 description 8
- 210000003714 granulocyte Anatomy 0.000 description 8
- 210000001616 monocyte Anatomy 0.000 description 8
- 238000007664 blowing Methods 0.000 description 5
- 230000001413 cellular effect Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 238000007599 discharging Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 230000002949 hemolytic effect Effects 0.000 description 3
- 230000004931 aggregating effect Effects 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000010808 liquid waste Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention is suitable for the technical field of sample analysis, and discloses a cell analyzer and a cell detection method suitable for sheep, goats and cows. The cell analyzer comprises an analyzer execution main body, a man-machine interaction device and a controller; the controller is configured to: when the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer to execute the main body to dilute the blood sample to a first preset proportion value through the diluent and the hemolytic agent to prepare a first detection sample liquid, controlling the analyzer to execute the main body to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method, wherein the first type animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode, and the first preset proportion value is larger than or equal to 1200:1 and less than or equal to 3500:1. the invention realizes accurate detection of the white blood cell parameters of sheep, goat or cow blood samples by adopting an impedance method.
Description
Technical Field
The invention relates to the technical field of sample analysis, in particular to a cell analyzer and a cell detection method suitable for sheep, goats and cows.
Background
The cell analyzer provided by the traditional technology adopts the expensive optical detection device to detect the white blood cell parameters of the blood sample of the animal, can be suitable for detecting the white blood cell parameters of the blood sample of dogs, cats, horses, sheep, goats and cows, and has higher accuracy of detection results. However, the cell analyzer is not suitable for popularization and application in small and medium medical places and small laboratories because of the optical detection device with a complex structure and high cost.
In order to solve the above technical problems, a related art proposes a scheme for detecting leukocyte parameters of an animal blood sample by using an impedance detection channel, and the specific implementation manner of the scheme is as follows: diluting a blood sample to 250-500 by adopting a diluent and a hemolytic agent in a white blood cell counting pool to prepare a detection sample liquid, and then performing white blood cell counting detection on the detection sample liquid in the white blood cell counting pool by adopting an impedance method through a detection device. When the testing method is used for testing the leukocyte parameters of blood samples of dogs, cats and horses, relatively accurate testing results can be obtained, and the cost of a cell analyzer for testing dogs, cats and horses can be greatly reduced. However, when the testing method is used for detecting the white blood cell parameters of the blood samples of sheep, goats and cows, the accuracy of the detection results is extremely poor, and the requirements of the white blood cell detection of the blood samples of sheep, goats and cows cannot be met. Therefore, in the prior art, basically, a cell analyzer with a costly optical detection device is used to detect the leukocyte parameters of blood samples of sheep, goats and cows.
Disclosure of Invention
The invention aims to provide a cell analyzer suitable for sheep, goats and cows, which aims to solve the technical problem of high cost of the cell analyzer for detecting sheep, goats and cows white blood cells in the prior art.
In order to achieve the above purpose, the invention provides the following scheme: a cell analyzer suitable for sheep, goats and cows comprises an analyzer execution body, a man-machine interaction device and a controller;
the analyzer execution body is configured to be capable of executing a white blood cell count detection item on a blood sample using an impedance method;
the human-machine interaction device is configured to be able to select an animal detection mode as follows: a first type of animal detection mode and a second type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
according to the animal detection mode selected by the man-machine interaction device, controlling the analyzer to execute the following actions on the blood sample by the execution main body:
when the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute a blood sample to a first preset proportion value through a diluent and a hemolytic agent to prepare a first detection sample liquid, and controlling the analyzer execution main body to perform white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of the white blood cells;
When the animal detection mode selected by the man-machine interaction device is a second-class animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a second preset proportion value through diluent and hemolytic agent to prepare a second detection sample liquid, and controlling the analyzer execution main body to perform white blood cell count detection on the second detection sample liquid by adopting an impedance method;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1, wherein the second preset ratio value is greater than or equal to 100:1 and less than or equal to 500:1.
a second object of the present invention is to provide a cell analyzer suitable for sheep, goats and cows, comprising an analyzer execution body, a man-machine interaction device and a controller;
the analyzer execution body is configured to be capable of executing a white blood cell count detection item on a blood sample using an impedance method;
the human-computer interaction device is configured to be capable of selecting a first type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
according to the animal detection mode selected by the man-machine interaction device, controlling the analyzer to execute the following actions of the main body on the blood sample:
When the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample liquid, and controlling the analyzer execution main body to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1.
a third object of the present invention is to provide a cell detection method suitable for sheep, goats and cows, comprising the steps of:
a sampling step of controlling a sampling device to collect a blood sample in a blood sample container;
a first sampling step of controlling the sampling device to convey at least part of the collected blood sample into the white blood cell counting cell;
a first detection sample liquid preparation step, namely controlling a diluent supply device to convey diluent into the white blood cell counting tank, controlling a hemolytic agent supply device to convey a hemolytic agent into the white blood cell counting tank so as to dissolve red blood cells in the blood sample, and diluting the blood sample to a first preset proportion value to prepare a first detection sample liquid;
A first detection step of controlling a first detection device to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of the white blood cells;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1.
the invention provides a cell analyzer and a cell detection method suitable for sheep, goats and cows, which are characterized in that a blood sample of sheep, goats or cows is diluted to be more than or equal to 1200 by diluent and hemolytic agent: 1 and less than or equal to 3500: in the range of 1 (the dilution ratio is far greater than that when the impedance method is adopted to detect cats, dogs and Ma Xieyang), preparing a first detection sample liquid, and then adopting the impedance method to carry out white cell count detection on the first detection sample liquid, so as to obtain relatively accurate white cell count values of sheep, goats or cows, thereby realizing the effect of accurately detecting the white cell count values of sheep, goats and cows by adopting the impedance method. Because the cost of the impedance detection device for impedance counting detection is far lower than that of the optical detection device for optical counting detection, the invention can greatly reduce the cost of the cell analyzer for detecting sheep, goat and cow leucocytes, and is favorable for large popularization and application in middle and small medical places and middle and small laboratories.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the composition of a cell analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the components of an analyzer execution body according to an embodiment of the present invention;
FIG. 3 is a schematic diagram showing the pulse change of a cell passing through a detection well according to an embodiment of the present invention;
FIG. 4 is a second histogram provided by an embodiment of the present invention;
FIG. 5 is a first histogram provided by an embodiment of the present invention;
FIG. 6 is a third histogram provided by an embodiment of the present invention;
fig. 7 is a histogram of sheep white blood cells detected using a horse detection mode, according to an embodiment of the present invention.
Reference numerals illustrate:
100. an analyzer execution body; 110. a white blood cell count cell; 111. a detection hole; 112. a first chamber; 113. a second chamber; 120. a first detection device; 121. a constant current source; 122. an electrode; 130. a negative pressure device; 140. a sampling device; 200. a man-machine interaction device; 300. and a controller.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indications (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship between the components, the movement condition, etc. in a specific posture, and if the specific posture is changed, the directional indication is changed accordingly.
It will also be understood that when an element is referred to as being "mounted" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or be indirectly connected to the other element through intervening elements.
Furthermore, the description of "first," "second," etc. in this disclosure is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
Prior to the present application, a cell analyzer for measuring white blood cell count parameters of animals by impedance method was basically used only for detecting animals such as cats, dogs and horses; when used for white blood cell count detection of sheep, goats and cows, the detection value is quite different from the actual value. Thus, prior to the present application, white blood cell count detection of sheep, goats, cows was essentially performed by a cell analyzer with a costly optical detection device.
In research of implementing sheep, goat and cow white blood cell count detection based on impedance method, those skilled in the art focus on the technical route of improving hemolysis agent, but no remarkable effect is obtained. In the research of realizing the white blood cell count detection of sheep, goats and cows based on an impedance method, the inventor breaks the conventional thinking, focuses on the technical route of greatly changing the dilution ratio of blood samples, and provides the effect of accurately detecting the white blood cell parameters of sheep, goats and cows by adopting the impedance count detection method with high dilution ratio (far higher than that of cats, dogs and horses) to perform the white blood cell count detection on the blood samples of sheep, goats and cows.
Whereas prior to the present application, the person skilled in the art would not have substantially thought of a substantial change in the dilution ratio of blood samples, which would enable the improvement of the white blood cell count detection values for sheep, goats and cows, because:
1) Before the application, in a cell analyzer for detecting animal white blood cell counting parameters by adopting an impedance method, the dilution ratio of blood samples of all animals is basically the same and is set between 250 and 500, and if the dilution ratio is lower than 250, the linearity of white blood cells is affected, so that the accuracy of a high-value white blood cell (white blood cell with a large number) test result is affected; on the other hand, if the dilution ratio is more than 500, the accuracy of the test result of the low-value white blood cells (white blood cells of a small number) is affected, and the reproducibility of the test result is affected (the result of the multiple tests is very different).
2) Furthermore, the applicant found during the course of the study that: in the white blood cell impedance counting detection of animals such as cats, dogs, horses and the like, a relatively accurate white blood cell count value can be obtained by setting the dilution ratio between 250 and 500, and if the dilution ratio is increased to 800, 1200, 2400 or even 3000, the count value is not changed obviously.
3) While the applicant also found during the course of the study that: in the white blood cell impedance counting detection of animals such as sheep, goats, cows and the like, if the dilution ratio is selected in the same or close (between 250 and 500) range as that of animals such as cats, dogs, horses and the like, after a hemolytic agent is added, the volume of red blood cell fragments formed after the rupture of red blood cells is obviously larger and is close to that of lymphocytes, so that the volumes of the red blood cell fragments and the lymphocytes coincide, the accurate counting of white blood cells cannot be realized, and the measured white blood cell count value and the actual value (which can be obtained by adopting an optical detection device) are greatly different; whereas if the dilution ratio is only increased to a small extent, e.g. to 550, 600, 650, 700, 800, etc., the measured white blood cell count value still differs significantly from the actual value.
The impedance method counting detection principle of the cells is as follows: a detection hole (i.e. a micropore) is arranged at the side part of the white cell counting cell, and an electrode is respectively arranged at the channels at the two sides of the detection hole to form a positive electrode and a negative electrode, and the two electrodes are connected to a constant current source. When the detection sample liquid to be detected flows through the detection hole under the action of constant negative pressure, the resistance between the positive electrode and the negative electrode changes, the resistance can form a pulse change proportional to the cell volume of the blood sample, and a particle distribution curve reflecting the cell size of the blood sample can be drawn by collecting the data of the pulse change, so that the detection data of the cells can be obtained. The impedance method is adopted for white blood cell count detection, and compared with the optical detection method, the impedance method has the advantages of low cost and simple structure.
Embodiment one:
as shown in fig. 1 to 7, a cell analyzer suitable for sheep, goats and cows according to an embodiment of the present invention includes an analyzer executing body 100, a man-machine interaction device 200 and a controller 300. The analyzer execution body 100 is configured to be able to execute at least one test item on a blood sample. The human-computer interaction device 200 is configured to have an animal detection mode selection function, so that a user can select a corresponding detection mode according to actual situations. The controller 300 is configured to control the analyzer execution body 100 to perform a corresponding test item on the blood sample according to the animal test mode selected by the human-computer interaction device 200. In a specific application, when the user selects a corresponding animal detection mode on the human-computer interaction device 200, the human-computer interaction device 200 feeds back a corresponding mode selection signal to the controller 300, and the controller 300 controls the analyzer execution body 100 to detect the blood sample according to the corresponding detection mode according to the received mode selection signal.
As one embodiment, the analyzer execution body 100 is configured to be able to perform a white blood cell count detection item on a blood sample using an impedance method, i.e., the cell analyzer is able to perform white blood cell impedance count detection on the blood sample.
The human-machine interaction device 200 is configured to be able to select the following animal detection mode: a first type of animal detection mode and a second type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode. In this embodiment, the cell analyzer can be used for performing white blood cell impedance count detection on blood samples of at least one animal selected from sheep, goats, and cows.
The controller 300 is configured to: according to the animal detection mode selected by the human-computer interaction device 200, the analyzer execution body 100 is controlled to execute the following actions on the blood sample:
when the animal detection mode selected by the man-machine interaction device 200 is the first type animal detection mode, controlling the analyzer execution body 100 to dilute the blood sample to a first preset proportion value through the diluent and the hemolytic agent to prepare a first detection sample liquid, and controlling the analyzer execution body 100 to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of white blood cells;
When the animal detection mode selected by the man-machine interaction device 200 is the second type animal detection mode, controlling the analyzer execution main body 100 to dilute the blood sample to a second preset ratio value through the diluent and the hemolytic agent to prepare a second detection sample liquid, and controlling the analyzer execution main body 100 to perform white blood cell count detection on the second detection sample liquid by adopting an impedance method;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1, the second preset ratio value is greater than or equal to 100:1 and less than or equal to 500:1.
the hemolytic agent is mainly used for dissolving red blood cells in a blood sample so as to avoid interference of the red blood cells on the white blood cell count, thereby being beneficial to improving the accuracy of the white blood cell count; in addition, the hemolysis agent may also act to dilute the blood sample. The diluent is mainly used for diluting blood sample cells so as to prevent blood sample cells from aggregating and adhering.
According to the cell analyzer provided by the embodiment, the blood sample of sheep or goat or cow is made into the first detection sample liquid with high dilution ratio (far higher than that of cat, dog and horse) through the diluent and the hemolytic agent, and then the white blood cell count detection is carried out on the first detection sample liquid by adopting the impedance method, so that the relatively accurate white blood cell count value of the blood sample of sheep or goat or cow can be obtained, and the effect of accurately detecting the white blood cell count value of sheep, goat and cow by adopting the impedance method is realized. Since the first detection device 120 for impedance counting detection has a cost far lower than that of the optical detection device for optical counting detection, the present embodiment can greatly reduce the cost of the cell analyzer for detecting white blood cells of sheep, goats and cows, which is beneficial for mass popularization and application in small and medium medical facilities and small and medium laboratories.
As an embodiment, the second type of animal detection mode includes at least one of a cat detection mode, a dog detection mode, and a horse detection mode, i.e., a dog, cat, and horse leukocyte detection mode, and the second type of animal detection mode may be employed.
In one embodiment, the second preset ratio is 250 or more and 500 or less, and in this range, the accuracy of the detection of the white blood cells in dogs, cats and horses is high, and the repeatability of the detection results is good (i.e., the difference between the results of multiple detections is small). If the dilution ratio is less than 250, the linearity of the white blood cells is affected, so that the accuracy of the test result of the high-value white blood cells (the white blood cells with a large number) is affected; if the dilution ratio is more than 500, the accuracy of the test result of the low-value white blood cells (small-number white blood cells) is affected, and the reproducibility of the test result is affected.
As a preferred implementation of this example, the cell analyzer is configured to detect white blood cells of dogs, cats, horses, sheep, goats, cows, and the human interaction device 200 is configured to select the following animal detection mode: the controller 300 is configured to control the analyzer execution body 100 to perform white blood cell parameter detection on a blood sample of sheep, goat, cow, dog, cat, and horse by impedance method, respectively. In the sheep detection mode, the goat detection mode and the cow detection mode, when white blood cell count detection is carried out on the blood sample, the blood sample is diluted to be more than or equal to 1200 by adopting diluent and hemolytic agent: 1 and less than or equal to 3500: 1. In the cat detection mode, the dog detection mode and the horse detection mode, when the white blood cell count detection is carried out on the blood sample, the blood sample is diluted to be more than 250 by adopting diluent and hemolysis agent: 1 and equal to or greater than 500: 1. Of course, in a specific application, the human-computer interaction device 200 is configured to select an animal detection mode, and the animal detection mode is not limited thereto, and may include only two or three or four or five animal detection modes, or the first type of animal detection mode and the second type of animal detection mode may also include only other animal detection modes.
As one embodiment, the first preset ratio value is greater than or equal to 1500:1 and less than or equal to 3000:1. the blood sample of sheep or goat or cow is diluted to the range, so that when the white blood cell count value of the blood sample of sheep, goat or cow is detected by adopting an impedance method, the detection result is more accurate, and the error is in a controllable range.
As a preferred implementation of this example, the first preset ratio value is (2400±200): 1, in the dilution range, the white blood cell count value of the blood sample of sheep, goats and cows is detected by adopting an impedance method, and the accuracy of the detection result value is very high.
As one embodiment, the analyzer execution body 100 includes a sampling device 140, a diluent supply device, a hemolysis agent supply device, a white blood cell count cell 110, and a first detection device 120, the first detection device 120 being for performing white blood cell count detection. The first detection device 120 is a resistive detector, the first detection device 120 comprises a constant current source 121, an analysis circuit and two electrodes 122, the white cell counting cell 110 is provided with a first chamber 112, a second chamber 113 and a detection hole 111, the first chamber 112 is a main chamber of the white cell counting cell 110, the dilution treatment of the blood sample is carried out in the first chamber 112, the second chamber 113 is connected with a negative pressure device 130, the detection hole 111 is communicated with the first chamber 112 and the second chamber 113, and the detection hole 111 is a micropore with smaller pore diameter. Two electrodes 122 are provided in the first chamber 112 and the second chamber 113, respectively, and a constant current source 121 is connected to the two electrodes 122. When the cells pass through the detection hole 111 under the action of the negative pressure device 130, corresponding pulses are generated, the larger the cell volume is, the larger the resistance is increased when the cells pass through the detection hole 111, so that the generated pulses are larger, namely the pulse amplitude is proportional to the cell volume, the pulse frequency is proportional to the cell number, and an analysis circuit can draw a particle distribution curve reflecting the cell size of the blood sample by collecting the pulse change data, so that the detection data of the cells are obtained. The white blood cell counting cell 110 is used for preparing the blood sample into a first detection sample liquid, and white blood cell counting detection is directly performed in the white blood cell counting cell 110, namely, the white blood cell counting cell 110 is used for providing a reaction (dilution, hemolysis, uniform mixing and the like) place for the blood sample and a detection place for white blood cell counting detection of the blood sample, so that the number, cost and volume of components of the cell analyzer are reduced.
As one embodiment, the sampling device 140 includes a sampling needle, a sample suction line, a suction and discharge driving device, and a movement driving device. The sampling pipe is respectively communicated with the sampling needle and the liquid sucking and discharging driving device. The aspiration and drainage drive device may provide a driving force for aspirating fluid for the sampling needle and may provide a driving force for discharging fluid for the sampling needle. The sampling needle is arranged on the motion driving device, and the motion driving device can drive the sampling needle to perform space motion so as to enable the sampling needle to move to different stations, such as waiting machine positions, sampling positions, sample dividing positions and the like.
As an embodiment, the analyzer execution body 100 is further configured to be able to execute a leukocyte classification detection item on a blood sample, that is, in this embodiment, the cell analyzer can accurately detect leukocyte classification data of sheep or goats or cows in addition to the leukocyte count value of sheep or goats or cows. Specifically, the controller 300 is configured to: when the animal detection mode selected by the human-computer interaction device 200 is the first type of animal detection mode, the analyzer is controlled to execute the following actions by the main body 100: diluting the blood sample to a third preset proportion value through diluent and hemolysis agent to prepare a third detection sample liquid; performing white blood cell count detection on at least part of the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells; diluting at least part of the third detection sample liquid to a first preset proportion value through a hemolysis agent and a diluent to prepare a first detection sample liquid; performing white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data; wherein the third preset ratio value is greater than or equal to 1200:1 and less than a first predetermined ratio value. In this embodiment, the third detection sample solution is mainly used for classifying and detecting white blood cells, and the hemolytic agent added in the preparation process can dissolve red blood cells, and can divide white blood cells into three groups, namely a lymphocyte group, a monocyte group and a granulocyte group, under the action of the hemolytic agent, so as to be beneficial to realizing the classification of white blood cells. The first detection sample liquid is mainly used for counting and detecting white blood cells. Wherein the first hemolytic agent added in preparing the third detection sample liquid is preferably not influencing the classification of white blood cells; the hemolytic agent added for the second time in preparing the first test sample liquid is preferably such that no red blood cell fragments are visible in the histogram. According to the embodiment, the blood sample is diluted, hemolyzed and detected twice, so that the classification and counting detection of the white blood cells can be realized, and the accuracy of the classification, counting and detection of the white blood cells is improved. Of course, in specific applications, as an alternative embodiment, it is not necessary to prepare a third test sample solution and perform white blood cell count test on the third test sample solution, but only the first test sample solution obtained by diluting a blood sample is performed on the cell analyzer suitable for sheep, goats and cows.
In this embodiment, the first test sample liquid is obtained by further lysing red blood cells and diluting the third test sample liquid, which is advantageous in reducing the consumption of blood sample, diluent and hemolysis agent. In addition, the first detection sample liquid and the third detection sample liquid are prepared in the same white blood cell counting cell 110, and a preparation place for the third detection sample liquid is not needed to be provided independently, so that the low-cost and small-volume design of the cell analyzer is facilitated. Of course, in a specific application, as an alternative embodiment, the preparation of the first test sample solution and the preparation of the third test sample solution may be performed independently of each other, that is, the preparation process of the first test sample solution is to perform the process of dissolving red blood cells and diluting one blood sample to prepare the first test sample solution, and the preparation process of the third test sample solution is to perform the process of dissolving red blood cells and diluting another blood sample to prepare the third test sample solution.
As one embodiment, the controller 300 is further configured to: and analyzing and processing the first detection data and the second detection data to obtain the classifying and counting data of the white blood cells. In this embodiment, the first detection data can obtain an accurate white blood cell count value, and the first detection data and the second detection data can be analyzed to obtain an accurate white blood cell classification count value.
Referring to fig. 4 to 6, the first detection data includes a first histogram (i.e., fig. 5), which is a histogram of the number and volume distribution of white blood cells, and a first count value of white blood cells; the second detection data comprises a second histogram (i.e. fig. 4) and a second count value of white blood cells, wherein the second histogram is a histogram of the number and volume distribution of red blood cell fragments and white blood cells; the controller 300 performs analysis processing on the first detection data and the second detection data, and embodiments for obtaining the differential count data of the white blood cells include: calculating S1 and S2 (i.e. S1 and S2 in fig. 4) according to the formula S1/(s1+s2) =wbc1/wbc2, determining the boundary between the red cell debris and the white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram through a dividing line to obtain a third histogram (i.e. fig. 6); classifying and counting the white blood cells in the third histogram according to the volume size to obtain classifying and counting data of the white blood cells; wherein S1 is the area of the distribution area of the white blood cells in the first histogram, S2 is the area of the distribution area of the red blood cell fragments in the second histogram, WBC1 is the first count value, and WBC2 is the second count value. The first histogram obtains an accurate white blood cell count value, but it cannot obtain a classification value of white blood cells; the cells in the second histogram may be classified into lymphocyte populations, monocyte populations and granulocyte populations according to the volume size of the cells, but the lymphocyte populations contain red blood cell fragments, so the count value is higher than the actual number of white blood cells. In this embodiment, according to the formula S1/(s1+s2) =wbc1/wbc2, the red blood cell fragments in the second histogram are removed to obtain a third histogram, and according to the volume size of the cells, lymphocytes, monocytes and granulocytes can be classified, so that an accurate white blood cell classification count value can be obtained.
In this example, the classification of white blood cells can be obtained by diluting the blood sample with a first hemolyzing agent and a diluent, but since it contains red blood cell fragments, its count is less accurate; further dilution of the blood sample by the second hemolysis agent and diluent can further reduce the red blood cell fragments so that the red blood cell fragments are not visible in the histogram, thereby obtaining an accurate white blood cell classification count value.
As one embodiment, the third preset ratio value is greater than or equal to 1200:1 and less than or equal to 2000:1, in the range, the more accurate leucocyte classification detection data can be obtained.
As a preferred implementation of this example, the third preset ratio value is (1800±200): 1.
as one embodiment, the analyzer execution body 100 is further configured to be able to execute a red blood cell count detection item on a blood sample, the analyzer execution body 100 further comprising a red blood cell count cell and a second detection means for performing red blood cell count detection;
the controller 300 is configured to: when the animal detection mode selected by the human-computer interaction device 200 is the first type of animal detection mode, the analyzer is controlled to execute the following actions by the main body 100:
Controlling the sampling device 140 to collect a blood sample and adding the collected blood sample to the white blood cell count cell 110;
controlling a diluent supply device to add diluent into the white blood cell counting cell 110 so as to dilute the blood sample to a fourth preset proportion value, thereby preparing a fourth detection sample liquid;
the sampling device 140 is controlled to collect a first part of fourth detection sample liquid from the white blood cell counting cell 110, and the sampling device 140 is controlled to add the collected first part of fourth detection sample liquid into the red blood cell counting cell for dilution and red blood cell counting detection;
controlling the sampling device 140 to collect a second portion of the fourth test sample fluid from the white blood cell count cell 110;
the analyzer execution body 100 may further include a liquid discharge pipeline, a liquid discharge valve and a liquid waste tank, wherein the liquid discharge pipeline is connected between the white blood cell counting tank 110 and the liquid waste tank, the liquid discharge valve is arranged on the liquid discharge pipeline for controlling the on-off of the liquid discharge pipeline, and the white blood cell counting tank 110 can be emptied by controlling the opening of the liquid discharge valve;
controlling the sampling device 140 to add at least part of the fourth detection sample liquid in the collected second part of the fourth detection sample liquid into the white blood cell count cell 110;
Controlling a diluent supply device to convey diluent into the white blood cell counting cell 110, and controlling a hemolytic agent supply device to convey a hemolytic agent into the white blood cell counting cell 110 so as to dissolve red blood cells in the fourth detection sample liquid, and diluting the blood sample to a first preset proportion value to prepare a third detection sample liquid;
controlling the first detection device 120 to perform white blood cell count detection on the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells;
controlling the diluent supply device to convey diluent into the white blood cell counting cell 110, and controlling the hemolytic agent supply device to convey hemolytic agent into the white blood cell counting cell 110 so as to dilute the third detection sample liquid to a first preset proportion value, so as to prepare a first detection sample liquid;
controlling the first detection device 120 to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data;
the fourth preset proportion value is smaller than the third preset proportion value.
In this embodiment, the cell analyzer can accurately detect the red blood cell count value of sheep, goats or cows in addition to the white blood cell classification and count value of sheep, goats or cows. In addition, in this embodiment, the blood sample is diluted into the fourth test sample liquid by the dilution liquid in the white blood cell counting cell 110, then the first part of the fourth test sample liquid is collected by the sampling device 140 and distributed to the red blood cell counting cell, then the second part of the fourth test sample liquid is collected, then the remaining fourth test sample liquid in the white blood cell counting cell 110 is emptied, and at least part of the fourth test sample liquid in the sampling device 140 is added into the white blood cell counting cell 110 for dilution and hemolysis to prepare the test sample liquid for white blood cell detection.
In this embodiment, a part of the fourth test sample liquid is used for performing red blood cell count test, and a part of the fourth test sample liquid is used for preparing a third test sample liquid, that is, the third test sample liquid is obtained by further processing the fourth test sample liquid, so that the consumption of blood samples and diluent is reduced. In this embodiment, the first detection sample liquid, the third detection sample liquid, and the fourth detection sample liquid are prepared in the same white blood cell counting cell 110, and the preparation sites of the third detection sample liquid and the fourth detection sample liquid do not need to be provided separately, which is beneficial to realizing the low cost and small volume design of the cell analyzer. Of course, in a specific application, as an alternative embodiment, the preparation of the third test sample solution and the preparation of the fourth test sample solution may be performed independently of each other, that is, the preparation process of the third test sample solution is to perform hemolysis and dilution on one blood sample to obtain the third test sample solution, and the preparation process of the fourth test sample solution is to perform hemolysis and dilution on another blood sample to obtain the fourth test sample solution; as another alternative, the first test sample fluid may also be obtained by direct treatment of the fourth test sample fluid.
As one embodiment, the strand control is configured to: the sampling device 140 is controlled to add a part (i.e., the third part) of the collected second part of the fourth test sample liquid to the white blood cell count cell 110 for preparing the third test sample liquid and the first test sample liquid, instead of adding all of the collected second part of the fourth test sample liquid to the white blood cell count cell 110, so that it is advantageous to precisely control the volume of the fourth test sample liquid added to the white blood cell count cell 110. Of course, in a specific application, as an alternative embodiment, the sampling device 140 may also be controlled to completely add the collected second portion of the fourth detection sample liquid to the white blood cell count cell 110 for preparing the third detection sample liquid and the first detection sample liquid.
As one embodiment, the controller 300 is further configured to: the diluent supply means is controlled to add diluent to the white blood cell count cell 110 to form a base fluid before controlling the sampling means 140 to collect a blood sample and adding the collected blood sample to the white blood cell count cell 110.
In one embodiment, in the preparation process of the first test sample solution, after the hemolysis agent, the diluent and the blood sample are added to the white blood cell counting cell 110 in proportion, the hemolysis agent, the diluent and the blood sample are uniformly mixed. In a preferred embodiment, the bottom of the white blood cell counting cell 110 is provided with a vent connected with a gas path system, and the mixing operation is to blow gas from the bottom of the white blood cell counting cell 110; of course, in particular applications, other means of mixing, such as stirring with a stirring rod, may be used as an alternative embodiment.
In one embodiment, the preparation process of the third test sample solution and the preparation process of the fourth test sample solution also include a mixing operation, and a mixing manner may refer to a mixing manner in the preparation of the first test sample solution, which is not described in detail herein.
The embodiment also provides a cell detection method suitable for sheep, goats and cows, which comprises a first detection sample liquid preparation step and a first detection step. The first detection sample liquid preparation step comprises the following steps: diluting the blood sample to a first preset proportion value by adopting a hemolysis agent and a diluent to prepare a first detection sample liquid, wherein the first preset proportion value is larger than or equal to 1200:1 and less than or equal to 3500:1. the first detection step includes: and performing white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain count detection data of white blood cells. The hemolytic agent is mainly used for dissolving red blood cells in a blood sample so as to avoid interference of the red blood cells on the white blood cell count, thereby being beneficial to improving the accuracy of the white blood cell count; in addition, the hemolysis agent may also act to dilute the blood sample. The diluent is mainly used for diluting blood sample cells so as to prevent blood sample cells from aggregating and adhering. In the embodiment, the detection sample liquid with high dilution ratio is detected by adopting the impedance method, so that the accuracy of the white blood cell count value of the blood sample of sheep, goats and cows can be effectively improved on the premise of low cost.
Referring to fig. 1 to 3, as an embodiment, the first detecting step includes: the first detection device 120 is controlled to perform white blood cell count detection on the first detection sample liquid, so as to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of white blood cells. The principle of detecting the white blood cell count value by the impedance method by the first detecting means 120 may be referred to the above description in the cell analyzer, and will not be described in detail herein.
The preferred setting range of the first preset ratio value may be referred to the description in the above cellular analyzer, and will not be described in detail herein.
As an embodiment, the cell detection method suitable for sheep, goats and cows further comprises a sampling step and a first sample separation step. The sampling step is used to collect a blood sample. The first sampling step is used for conveying at least part of the blood sample collected in the sampling step to the white blood cell counting cell 110, namely distributing the blood sample to the white blood cell counting cell 110. The first test sample liquid preparing step is for diluting at least a part of the blood sample dispensed to the white blood cell count cell 110 in the first sample separating step to a first preset ratio value to prepare a first test sample liquid.
As an embodiment, the first detection step is used for performing white blood cell count detection on the first detection sample liquid in the white blood cell count cell 110. In this embodiment, the white blood cell count cell 110 is used to prepare a blood sample into a first test sample solution, and white blood cell count test is directly performed in the white blood cell count cell 110, that is, the white blood cell count cell 110 is used to provide a treatment site for dilution and hemolysis of the blood sample and a test site for white blood cell count test of the blood sample, which is beneficial to reducing the number of components, cost and volume of the cell analyzer.
As one embodiment, the cell detection method applicable to sheep, goats and cows further comprises a third detection sample liquid preparation step and a second detection step. The third detection sample liquid preparation step comprises the following steps: diluting at least part of the blood sample to a third preset proportion value by adopting a hemolysis agent and a diluent to prepare a third detection sample liquid, wherein the third preset proportion value is larger than or equal to 1200:1 and less than a first predetermined ratio value. The second detection step comprises: the first detection device 120 is controlled to perform white blood cell count detection on at least part of the third detection sample liquid by adopting an impedance method, so as to obtain second detection data. The third detection sample liquid is mainly used for classifying and detecting white blood cells, and besides the hemolytic agent added in the preparation process can dissolve red blood cells, white blood cells can be divided into three groups of lymphocyte groups, monocyte groups and granulocyte groups under the action of the hemolytic agent, so that the classification of white blood cells is facilitated. The first detection sample liquid is mainly used for counting and detecting white blood cells. According to the embodiment, the blood sample is diluted and detected twice, so that the classification and counting detection of the white blood cells can be realized, and the accuracy of the classification and counting detection of the white blood cells is improved. Of course, in specific applications, as an alternative embodiment, the cell detection method suitable for sheep, goats and cows does not necessarily require the third detection sample liquid preparation step and the second detection step, but only performs counting detection on the blood sample.
As an embodiment, the first detection sample liquid preparation step is performed by: and diluting at least part of the third detection sample liquid to a first preset proportion value by adopting a diluent and a hemolytic agent to prepare a first detection sample liquid, namely in the embodiment, the first detection sample liquid is obtained by further dissolving red blood cells and diluting the third detection sample liquid, so that the consumption of blood samples, the diluent and the hemolytic agent is reduced. Of course, in a specific application, as an alternative embodiment, the first test sample liquid preparation step and the third test sample liquid preparation step may be performed independently of each other, that is, the first test sample liquid preparation step performs red blood cell lysis and dilution on one blood sample to obtain the first test sample liquid, and the third test sample liquid preparation step performs red blood cell lysis and dilution on another blood sample to obtain the third test sample liquid.
The preferred setting range of the third preset ratio value may be referred to the description in the above-mentioned cellular analyzer, and will not be described in detail herein.
As one embodiment, the cell detection method further comprises a data analysis step including: and analyzing and processing the first detection data and the second detection data to obtain the classifying and counting data of the white blood cells. In this embodiment, the first detection data can obtain an accurate white blood cell count value, and the first detection data and the second detection data can be analyzed to obtain an accurate white blood cell classification count value.
Referring to fig. 4 to 6, as an embodiment, the first detection data includes a first histogram (i.e., fig. 5), which is a histogram of the number and volume distribution of white blood cells, and a first count value of white blood cells; the second detection data comprises a second histogram (i.e. fig. 4) and a second count value of white blood cells, wherein the second histogram is a histogram of the number and volume distribution of red blood cell fragments and white blood cells; the data analysis step includes: calculating S1 and S2 (i.e. S1 and S2 in fig. 4) according to the formula S1/(s1+s2) =wbc1/wbc2, determining the boundary between the red cell debris and the white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram through a dividing line to obtain a third histogram (i.e. fig. 6); classifying and counting the white blood cells in the third histogram according to the volume size to obtain classifying and counting data of the white blood cells; wherein S1 is the area of the distribution area of the white blood cells in the first histogram, S2 is the area of the distribution area of the red blood cell fragments in the second histogram, WBC1 is the first count value, and WBC2 is the second count value. The first histogram obtains an accurate white blood cell count value, but it cannot obtain a classification value of white blood cells; the cells in the second histogram may be classified into lymphocyte populations, monocyte populations and granulocyte populations according to the volume size of the cells, but the lymphocyte populations contain red blood cell fragments, so the count value is higher than the actual number of white blood cells. In this embodiment, according to the formula S1/(s1+s2) =wbc1/wbc2, the red blood cell fragments in the second histogram are removed to obtain a third histogram, and according to the volume size of the cells, lymphocytes, monocytes and granulocytes can be classified, so that an accurate white blood cell classification count value can be obtained.
As one embodiment, the cell detection method further includes a fourth detection sample liquid preparation step and a third detection step, the fourth detection sample liquid preparation step including: and diluting the blood sample to a fourth preset proportion value by adopting a diluent to prepare a fourth detection sample liquid. The third detection step includes: and diluting at least a part of the fourth detection sample liquid to prepare a fifth detection sample liquid, and controlling the second detection device to perform red blood cell counting detection on the fifth detection sample liquid. In this embodiment, the cell detection method can realize both the differential count detection of white blood cells and the count detection of red blood cells.
As an embodiment, the fourth test sample liquid preparation step is performed after the first sample separation step and before the third test sample liquid preparation step. The cell detection method further comprises a second sample separation step after the fourth detection sample liquid preparation step and before the third detection step, the second sample separation step comprising: and delivering the fourth detection sample liquid of the third part to a red blood cell counting cell. The third detection sample liquid preparation step is implemented as follows: and dissolving the fourth detection sample liquid of the first part by adopting a diluent and a hemolytic agent, and diluting the fourth detection sample liquid of the first part to a third preset proportion value to prepare a third detection sample liquid, wherein the fourth preset proportion value is smaller than the third preset proportion value. In this embodiment, a part of the fourth test sample liquid is used for performing the red blood cell count test, and a part of the fourth test sample liquid is used for preparing the third test sample liquid, that is, the third test sample liquid is obtained by further processing the fourth test sample liquid, so that the consumption of the blood sample and the dilution liquid is reduced. Of course, in a specific application, as an alternative embodiment, the third test sample liquid preparation step and the fourth test sample liquid preparation step may be performed independently of each other, that is, the third test sample liquid preparation step hemolyzes and dilutes one blood sample to obtain a third test sample liquid, and the fourth test sample liquid preparation step hemolyzes and dilutes another blood sample to obtain a fourth test sample liquid; as another alternative, the first test sample fluid may also be obtained by direct treatment of the fourth test sample fluid.
In one embodiment, the second sampling step further comprises, after transferring the fourth test sample liquid of the third portion to the red blood cell counting cell: sucking a first portion of the fourth test sample liquid from the white blood cell count cell 110 by the sampling device 140; discharging the blank cell counting cell 110, and adding a diluent into the blank cell counting cell 110 to form a base solution; the fourth test sample fluid of the first part of the sampling device 140 is transferred to the white blood cell test cell. The method of re-diluting part of the sample liquid after emptying is adopted instead of diluting all the detection sample liquid, which is beneficial to reducing the dosage of the diluent and reducing the volume of the white cell counting cell 110.
Referring to fig. 1 to 3, as an embodiment, the sampling step includes: the sampling device 140 is controlled to collect a blood sample in a blood sample container. The specific structure of the sampling device 140 may be described in the above-described cellular analyzer, and will not be described in detail herein. When the sampling step is implemented, the movement driving device drives the sampling needle to move to the upper part of the blood sample container, then drives the sampling needle to extend into the blood sample container and be inserted into the blood sample in the blood sample container, the liquid sucking and discharging driving device drives the sampling needle to suck the blood sample, and then the movement driving device drives the sampling needle to move away from the blood sample container, so that the sampling step is completed.
As one embodiment, the first decimating step includes: the sampling device 140 is controlled to deliver at least a portion of the collected blood sample to the white blood cell count cell 110. When the first sample separation step is implemented, the motion driving device drives the sampling needle to move to the upper part of the white cell counting pool 110, then drives the sampling needle to extend into the white cell counting pool 110, the liquid sucking and discharging driving device drives the sampling needle to discharge a blood sample, and then the motion driving device drives the sampling needle to move away from the white cell counting pool 110, so that the first sample separation step is completed.
As an embodiment, between the sampling step and the first decimating step, further comprising the steps of: a diluent is added to the white blood cell count cell 110 to form a base fluid.
As an embodiment, the fourth detection sample liquid preparation step is performed by: the diluent supply device is controlled to deliver diluent to the white blood cell counting cell 110 so as to dilute the blood sample to a fourth preset proportion value, so that a fourth detection sample liquid is prepared. In this embodiment, the fourth test sample solution is prepared in the white blood cell counting cell 110, and the preparation site of the fourth test sample solution is not required to be separately provided, which is advantageous in realizing low cost and small volume design of the cell analyzer.
As one embodiment, the third test sample liquid preparation step includes: the diluent supply device is controlled to deliver diluent to the white blood cell counting cell 110, and the hemolytic agent supply device is controlled to deliver hemolytic agent to the white blood cell counting cell 110 so as to dissolve red blood cells in the blood sample, and the blood sample is diluted to a third preset proportion value, so that a third detection sample liquid is prepared.
As one embodiment, the third test sample liquid preparation step is performed after the fourth test sample liquid preparation step and before the first test sample liquid preparation step, and includes: and dissolving red blood cells of the fourth detection sample liquid of the third part by adopting a diluent and a hemolytic agent, and diluting the fourth detection sample liquid of the third part to a third preset proportion value to prepare a third detection sample liquid. In this embodiment, the third detection sample liquid is prepared in the white blood cell counting cell 110, and the preparation site of the third detection sample liquid is not required to be separately provided, which is beneficial to realizing low cost and small volume design of the cell analyzer.
As a preferred implementation of this example, the second sampling step includes: controlling the sampling device 140 to collect a first portion of the third detection sample from the white blood cell counting cell 110, and controlling the sampling device 140 to add a collected first portion of the fourth detection sample liquid into the red blood cell counting cell; the third detection sample liquid preparation step comprises the following steps: the sampling device 140 is controlled to collect a second part of the third detection sample from the white blood cell counting cell 110, and the fourth detection sample liquid remained in the blank cell counting cell 110 is controlled; the diluent supply device is controlled to deliver diluent to the white blood cell counting chamber 110 to form a base solution, the sampling device 140 is controlled to add a third portion of a fourth detection sample solution (the third portion of the fourth detection sample solution belongs to at least a part of the second portion of the fourth detection sample solution collected by the sampling device 140) to the white blood cell counting chamber 110, the diluent supply device is controlled to deliver diluent to the white blood cell counting chamber 110, and the hemolysis agent supply device is controlled to deliver hemolysis agent to the white blood cell counting chamber 110 to dissolve red blood cells in a blood sample and dilute the blood sample to a third preset ratio value, so as to prepare a third detection sample solution. In this embodiment, the third detection sample volume of the second portion is greater than the third detection sample volume of the third portion; of course, in a specific application, the third detection sample volume of the second portion may also be set equal to the third detection sample volume of the third portion as an alternative embodiment.
As one embodiment, the second detecting step includes: the first detection device 120 is controlled to perform white blood cell count detection on the third detection sample liquid, so as to obtain second detection data. In a preferred embodiment of the present embodiment, the third test sample liquid preparation step, the second test step, and the first test sample liquid preparation step are sequentially performed in the same white blood cell count cell 110, that is, the second test step is performed after the third test sample liquid preparation step and before the first test sample liquid preparation step, so that the hemolytic agent added in the first test sample liquid preparation step is advantageously prevented from affecting the second test data detected by the second test step, thereby achieving the effect of improving the white blood cell classification count accuracy of sheep, goats, and cows on the premise of low cost.
As one embodiment, the first test sample liquid preparation step includes: the diluent supply device is controlled to deliver diluent to the white blood cell counting cell 110, and the hemolytic agent supply device is controlled to deliver hemolytic agent to the white blood cell counting cell 110 so as to dissolve red blood cells in the blood sample, and the blood sample is diluted to a first preset proportion value, so that a first detection sample liquid is prepared. In a preferred embodiment of this embodiment, the first test sample liquid preparation step is performed by: and releasing the third detection sample liquid after the second detection step to a third preset proportion value by adopting diluent and hemolytic agent to prepare the first detection sample liquid.
As an embodiment, an embodiment of the third detection step includes: and controlling the diluent supply device to convey diluent to the erythrocyte counting tank so as to dilute a third part of the fourth detection sample liquid in the erythrocyte counting tank to prepare a fifth detection sample liquid, and controlling the second detection device to perform erythrocyte counting detection on the fifth detection sample liquid. The third detection step may be started at any time period after the completion of the second sample separation step.
In one embodiment, in the first test sample liquid preparation step, after the hemolysis agent, the diluent and the blood sample are added to the white blood cell count cell 110 in proportion, the hemolysis agent, the diluent and the blood sample are mixed uniformly. The specific mixing method is described in the above-mentioned cellular analyzer, and will not be described in detail here.
In one embodiment, the third test sample liquid preparation step and the fourth test sample liquid preparation step further include a mixing operation, and the mixing method may refer to the mixing method in the first test sample liquid preparation step, and will not be described in detail herein.
As a preferred embodiment of the present example, the method for detecting blood samples in the second type of animal detection mode (i.e., the method for detecting cells in horses or dogs or cats) comprises the steps of:
Adding 1350uL of the diluent to the white blood cell count cell 110 to form a base fluid;
adding 9uL of blood sample into the white cell counting cell 110, and simultaneously adding 550uL of diluent, and flushing a sampling needle;
blowing air into the white blood cell counting pool 110 from the bottom of the white blood cell counting pool 110, and uniformly mixing the liquid in the white blood cell counting pool 110 to obtain a fourth detection sample liquid;
sucking out 20uL of the fourth detection sample liquid after uniform mixing, and adding the fourth detection sample liquid into a red blood cell counting tank for counting and detecting red blood cells;
at this time, the volume of the dilution in the white blood cell 110 is (1350+550)/(1350+550+9-20)/(1350+550+9) ≡ 1880.0943uL, and the volume of the blood sample is 9 (1350+550+9-20)/(1350+550+9) ≡ 8.9057uL;
300uL of hemolytic agent and 400uL of diluent were added to the white blood cell count cell 110, at which time the dilution ratio in the white blood cell count cell 110 was about (1880.0943+300+400)/8.9057 ≡289:1.
Blowing air into the white blood cell counting pool 110 from the bottom of the white blood cell counting pool 110, and uniformly mixing the liquid in the white blood cell counting pool 110 to prepare a second detection sample liquid;
and detecting the second detection sample liquid by adopting an impedance method to obtain blood sample leukocyte detection data of the horse, dog or cat.
If the method for detecting the white blood cells of the sheep blood sample in the second animal detection mode (i.e. the method for detecting the cells of the horse, the dog or the cat) is adopted, that is, the dilution ratio between 250 and 500 is adopted to detect the white blood cell parameters, the histogram shown in fig. 7 is obtained, and as can be seen from the graph, the volume of the red blood cell fragments is overlapped with that of the lymphocytes, and the red blood cell fragments cannot be filtered out from the histogram, so that the accurate test result of the white blood cells of the sheep blood sample cannot be obtained.
As a preferred embodiment of the present example, the method for detecting blood samples in the first type of animal detection mode (i.e., the method for detecting sheep or goat or cow cells) comprises the steps of:
adding 1350uL of the diluent to the white blood cell count cell 110 to form a base fluid;
adding 9uL of blood sample into the white cell counting cell 110, and simultaneously adding 550uL of diluent, and flushing a sampling needle;
blowing air into the white blood cell counting pool 110 from the bottom of the white blood cell counting pool 110, and uniformly mixing the liquid in the white blood cell counting pool 110 to obtain a fourth detection sample liquid;
sucking out 20uL of the fourth detection sample liquid after uniform mixing, and adding the fourth detection sample liquid into a red blood cell counting tank for counting and detecting red blood cells;
putting the sampling needle into the white cell counting cell 110 again, and sucking 320uL of fourth detection sample liquid into the sampling needle and a sample sucking pipeline connected with the sampling needle;
evacuating the white blood cell count cell 110;
1200mL of the diluent was added to the white blood cell count cell 110 to form a base fluid. Adding 305uL of liquid in the sampling needle and the sample suction pipeline into the white blood cell counting tank 110, and simultaneously adding 550uL of diluent to wash the sampling needle and the sample suction pipeline;
then 300uL of hemolytic agent and 400uL of diluent were added to the white blood cell 110, and the dilution ratio in the white blood cell 110 was about 1800:1. The function of the hemolytic agent is to lyse erythrocytes, while leukocytes are separated into several groups, such as lymphocyte groups, monocyte groups, granulocyte groups, etc., under the action of the hemolytic agent;
Blowing air into the white blood cell counting pool 110 from the bottom of the white blood cell counting pool 110, and uniformly mixing the liquid in the white blood cell counting pool 110 to prepare a third detection sample liquid;
detecting the third detection sample liquid to obtain second detection data; when the detection is carried out, the liquid in the white blood cell counting pool 110 passes through the detection hole 111 of the white blood cell counting pool 110 under the action of negative pressure, and the second detection data can be obtained by testing the signal of the white blood cell counting pool 110; the result of the count value of the white blood cells in the second detection data, which includes some red blood cell fragments, is higher than the actual value and is WBC2;
a further 300uL of hemolytic agent and 400uL of diluent were added to the white blood cell 110, at which point the dilution ratio of the white blood cell 110 was about 2400:1.
Blowing air into the white blood cell counting pool 110 from the bottom of the white blood cell counting pool 110, and uniformly mixing the liquid in the white blood cell counting pool 110 to prepare a first detection sample liquid.
And detecting the first detection sample liquid to obtain first detection data, wherein the first detection sample liquid is prepared by adding the hemolytic agent and the diluent again on the basis of the third detection sample liquid, so that the red cell fragments in the third detection sample liquid are further reduced under the action of the hemolytic agent, and the red cell fragments cannot be seen on the white cell histogram. The counting value of the white blood cells in the first detection data is consistent with the actual white blood cell value and is WBC1;
Removing red cell fragments on the left of a first counting histogram of white blood cells (namely, a second histogram obtained by detecting a third detection sample liquid (namely, fig. 4)) through WBC1 and WBC2, calculating S1 and S2 according to the formula S1/(S1+S2) =WBC1/WBC2, and determining the boundary between the red cell fragments and white blood cells in the second histogram according to the calculated S1 and S2; removing the distribution area of the red blood cell fragments in the second histogram through a dividing line to obtain a third histogram (i.e. fig. 6); the white blood cells in the third histogram are classified and counted according to the volume size to obtain the classified and counted data of the white blood cells, such as the classified and counted data of lymphocytes, monocytes and granulocytes. Wherein S1 is a distribution area of white blood cells in the first histogram, S2 is a distribution area of red blood cell debris in the second histogram, WBC1 is a first count value, and WBC2 is a second count value.
The applicant carried out the white blood cell impedance count test on the blood samples of the horses using different dilution ratios, the results obtained are as follows:
when the dilution ratio was 289:1, the white blood cell count value (WBC) was 8.22×10 9 /L；
When the dilution ratio is 400:1, the number value of the detected white blood cells is 8.32 x 10 9 /L；
When the dilution ratio is 800:1, the number value of the detected white blood cells is 8.01 x 10 9 /L；
When the dilution ratio is 1000:1, the number value of the detected white blood cells is 7.92 x 10 9 /L；
When the dilution ratio is 1800:1, the number value of the detected white blood cells is 8.02 x 10 9 /L；
When the dilution ratio is 2400:1, the value of the number of the detected white blood cells is 8.45*10 9 /L。
Wherein the actual count value of the white blood cells of the blood sample of the horse (measured by a cell analyzer with an optical detection device) is 8.22×10 9 and/L. It follows that the greater the dilution ratio, the more accurate the white blood cell impedance count of the blood sample of the horse.
The applicant carried out white blood cell impedance count tests on sheep blood samples using different dilution ratios, the results obtained were as follows:
when the dilution ratio was 289:1, the white blood cell number value was 95.62 x 10 9 /L；
When the dilution ratio is 500:1, the number value of the detected white blood cells is 85.12 x 10 9 /L；
When the dilution ratio is 1200:1, the number of the detected white blood cells is 26.85 x 10 9 /L；
When the dilution ratio is 1500:1, the number value of the detected white blood cells is 20.65 x 10 9 /L；
When the dilution ratio is 1800:1, the number value of the detected white blood cells is 19.36 x 10 9 /L；
When the dilution ratio is 2400:1, the number value of the detected white blood cells is 12.51 x 10 9 /L；
When the dilution ratio is 3000:1, the number value of the detected white blood cells is 13.02 x 10 9 /L；
When the dilution ratio is 3500:1, the number value of the detected white blood cells is 12.32 x 10 9 /L。
Wherein the sheep blood sample white blood cell actual count value (measured by a cell analyzer having an optical detection device) is 12.51×10 9 and/L. Therefore, when the white blood cell count value of the sheep blood sample is detected by adopting the impedance method, the accuracy of the detection result of the dilution ratio between 1200:1 and 3500:1 is higher than that of the detection result of the dilution ratio between 250:1 and 500:1, and when the dilution ratio is in a certain high dilution ratio range, the count value is relatively close to the actual count value.
The applicant carried out white blood cell impedance count detection on goat blood samples using different dilution ratios, and the results obtained were as follows:
when the dilution ratio is 289:1The number of the detected white blood cells is 65.32 x 10 9 /L；
When the dilution ratio is 500:1, the number of the detected white blood cells is 58.36 x 10 9 /L；
When the dilution ratio is 1200:1, the detected white blood cell number value is 43.56 x 10 9 /L；
When the dilution ratio is 1500:1, the number of the detected white blood cells is 29.68 x 10 9 /L；
When the dilution ratio is 1800:1, the number value of the detected white blood cells is 23.78 x 10 9 /L；
When the dilution ratio is 2400:1, the number of the detected white blood cells is 12.34 x 10 9 /L；
When the dilution ratio is 3000:1, the number value of the detected white blood cells is 11.68 x 10 9 /L；
When the dilution ratio is 3500:1, the number value of the detected white blood cells is 13.02 x 10 9 /L。
Wherein the actual count value of white blood cells of the blood sample of the goat (measured by a cell analyzer with an optical detection device) is 12.65×10 9 and/L. Therefore, when the white blood cell count value of the blood sample of the goat is detected by adopting the impedance method, the accuracy of the detection result of the dilution ratio between 1200:1 and 3500:1 is higher than that of the detection result of the dilution ratio between 250:1 and 500:1, and when the dilution ratio is in a certain high dilution ratio range, the count value is relatively close to the actual count value.
The applicant carried out white blood cell impedance count detection on blood samples of cows by adopting different dilution ratios, and the obtained results are as follows:
when the dilution ratio was 289:1, the white blood cell number value was detected to be 32.52 x 10 9 /L；
When the dilution ratio is 500:1, the number of the detected white blood cells is 23.56 x 10 9 /L；
When the dilution ratio is 1200:1, the number value of the detected white blood cells is 20.54 x 10 9 /L；
When the dilution ratio is 1500:1, the number value of the detected white blood cells is 18.98 x 10 9 /L；
When the dilution ratio is 1800:1, white blood cells are detectedNumber value 13.23 x 10 9 /L；
When the dilution ratio is 2400:1, the number value of the detected white blood cells is 13.65 x 10 9 /L；
When the dilution ratio is 3000:1, the number value of the detected white blood cells is 12.35 x 10 9 /L；
When the dilution ratio is 3500:1, the number value of the detected white blood cells is 12.39 x 10 9 /L。
Wherein the actual count value of white blood cells of blood sample of the dairy cow (measured by a cell analyzer with an optical detection device) is 12.35×10 9 and/L. Therefore, when the white blood cell count value of the blood sample of the dairy cow is detected by adopting the impedance method, the accuracy of the detection result of the dilution ratio between 1200:1 and 3500:1 is higher than that of the detection result of the dilution ratio between 250:1 and 500:1, and when the dilution ratio is in a certain high dilution ratio range, the count value is relatively close to the actual count value.
Embodiment two:
the cell detection method and the cell analyzer applicable to sheep, goats and cows provided in this embodiment are mainly different from those in the first embodiment in that: in the first embodiment, the cell analyzer can realize the differential counting detection of the white blood cells, and two detection sample liquids are obtained by adopting a method of twice diluting and dissolving the red blood cells, and the two detection sample liquids are respectively detected, so that the differential counting data of the white blood cells of the blood samples of sheep, goats and cows can be accurately detected; in this embodiment, the cell analyzer can realize the counting detection of the white blood cells, but cannot realize the classification detection of the white blood cells, and the method of diluting and dissolving the red blood cells once is adopted to obtain a first detection sample liquid, and the first detection sample liquid is detected, so that the white blood cell counting data of the blood samples of sheep, goats and cows can be accurately detected.
Specifically, in this embodiment, the cell detection method applicable to sheep, goats and cows includes a first detection sample liquid preparation step and a first detection step; and the third detection sample liquid preparation step and the second detection step are not included.
As an embodiment, the cell detection method further includes a sampling step and a first sample separation step, and specific embodiments of the sampling step and the first sample separation step may be referred to in example one, and will not be described in detail herein.
In one embodiment, the cell detection method further comprises a fourth detection sample liquid preparation step, a second sample separation step and a third detection step, wherein the first detection sample liquid is prepared by directly diluting, hemolyzing or preparing a part of the fourth detection sample liquid by using a diluent and a hemolyzing agent. For the specific embodiments of the fourth test sample liquid preparation step, the second sample separation step, and the third test step, reference is made to example one, and details thereof will not be described herein.
Specifically, the cell analyzer provided in the present embodiment also includes an analyzer execution body 100, a man-machine interaction device 200, and a controller 300; the analyzer execution body 100 is configured to be able to perform the following actions under the control of the controller 300: and preparing a first detection sample liquid from the blood sample by adopting a diluent and a hemolytic agent, and performing white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells. That is, in the present embodiment, the analyzer execution body 100 does not execute the third test sample liquid and the preparation and the test.
In addition to the above-mentioned differences, the cell detection method and other parts of the cell analyzer provided in this embodiment can be referred to as a corresponding optimization design in the embodiment, and will not be described in detail herein.
Embodiment III:
the difference between the cell analyzer provided in this embodiment and the first and second embodiments is mainly that the configurations of the man-machine interaction device 200 and the controller 300 are different, and the difference is specifically that: in the first and second embodiments, the human-computer interaction device 200 is configured to be capable of selecting a first type of animal detection mode and a second type of detection mode; in this embodiment, the human-computer interaction device 200 is configured to select only the first type of animal detection mode.
Specifically, in the present embodiment, the cell analyzer includes an analyzer execution body 100, a man-machine interaction device 200, and a controller 300; the analyzer execution body 100 is configured to be able to execute a leukocyte classification detection item and a leukocyte count detection item on a blood sample;
the human-machine interaction device 200 is configured to be able to select a first type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode, a cow detection mode;
the controller 300 is configured to: according to the animal detection mode selected by the human-computer interaction device 200, the analyzer is controlled to execute the following detection of the blood sample by the main body 100: when the animal detection mode selected by the man-machine interaction device 200 is the first type animal detection mode, controlling the analyzer execution body 100 to dilute the blood sample to a third preset ratio value through the diluent and the hemolytic agent to prepare a third detection sample liquid, and controlling the analyzer execution body 100 to perform white blood cell count detection on the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells; the method comprises the steps of controlling an analyzer executing body 100 to dilute a third detection sample liquid to a first preset proportion value through a diluting liquid and a hemolytic agent to prepare a first detection sample liquid, and controlling the analyzer executing body 100 to conduct white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of white blood cells; wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1, a third preset ratio value is greater than or equal to 1200:1 and less than a first predetermined ratio value.
In addition to the above differences, the other parts of the cellular analyzer provided in this embodiment can be referred to in the first embodiment and the second embodiment, and the detailed description thereof will not be given here.
The foregoing description is only of the preferred embodiments of the present invention and is not intended to limit the scope of the invention, and all equivalent structural changes made by the description of the present invention and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the invention.
Claims (15)
1. A cell analyzer suitable for sheep, goats and cows, characterized in that: the system comprises an analyzer execution main body, a man-machine interaction device and a controller;
the analyzer execution body is configured to be capable of executing a white blood cell count detection item on a blood sample using an impedance method;
the human-machine interaction device is configured to be able to select an animal detection mode as follows: a first type of animal detection mode and a second type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
according to the animal detection mode selected by the man-machine interaction device, controlling the analyzer to execute the following actions on the blood sample by the execution main body:
When the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute a blood sample to a first preset proportion value through a diluent and a hemolytic agent to prepare a first detection sample liquid, and controlling the analyzer execution main body to perform white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of the white blood cells;
when the animal detection mode selected by the man-machine interaction device is a second-class animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a second preset proportion value through diluent and hemolytic agent to prepare a second detection sample liquid, and controlling the analyzer execution main body to perform white blood cell count detection on the second detection sample liquid by adopting an impedance method;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1, wherein the second preset ratio value is greater than or equal to 100:1 and less than or equal to 500:1.
2. the cell analyzer for sheep, goats and cows as claimed in claim 1, wherein: the second type of animal detection mode includes at least one of a cat detection mode, a dog detection mode, and a horse detection mode.
3. A cell analyzer suitable for sheep, goats and cows, characterized in that: the system comprises an analyzer execution main body, a man-machine interaction device and a controller;
the analyzer execution body is configured to be capable of executing a white blood cell count detection item on a blood sample using an impedance method;
the human-computer interaction device is configured to be capable of selecting a first type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
according to the animal detection mode selected by the man-machine interaction device, controlling the analyzer to execute the following actions of the main body on the blood sample:
when the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample liquid, and controlling the analyzer execution main body to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1.
4. A cell analyser according to any one of claims 1 to 3 adapted for sheep, goats and cows, characterised in that: the first preset proportion value is greater than or equal to 1500:1 and less than or equal to 3000:1.
5. the cell analyzer for sheep, goats and cows of claim 4, wherein: the first preset proportion value is (2400+/-200): 1.
6. a cell analyser according to any one of claims 1 to 3 adapted for sheep, goats and cows, characterised in that: the analyzer execution body is further configured to be capable of executing a leukocyte classification test item on a blood sample using an impedance method;
the controller is configured to: when the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer to execute the following actions by the main body:
diluting the blood sample to a third preset proportion value through diluent and hemolysis agent to prepare a third detection sample liquid;
performing white blood cell count detection on at least part of the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells;
diluting at least part of the third detection sample liquid to the first preset proportion value through a hemolysis agent and a diluent to prepare a first detection sample liquid;
Performing white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data;
wherein the third preset ratio value is greater than or equal to 1200:1 and less than the first preset proportional value.
7. The cell analyzer for sheep, goats and cows of claim 6, wherein: the controller is further configured to: analyzing the first detection data and the second detection data to obtain the classified count data of the white blood cells;
the first detection data comprises a first histogram and the first count value of white blood cells, wherein the first histogram is a white blood cell number and volume distribution histogram; the second detection data comprises a second histogram and a second count value of white blood cells, wherein the second histogram is a histogram of the number and volume distribution of red blood cell fragments and white blood cells;
the controller analyzes and processes the first detection data and the second detection data to obtain the classifying and counting data of the white blood cells, and the embodiment comprises the following steps: calculating S1 and S2 according to the formula S1/(s1+s2) =wbc1/wbc2, determining the boundary between red cell fragments and white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram through the dividing line to obtain a third histogram; classifying and counting the white blood cells in the third histogram according to the volume size to obtain classifying and counting data of the white blood cells;
Wherein S1 is the area of the distribution area of the white blood cells in the first histogram, S2 is the area of the distribution area of the red blood cell fragments in the second histogram, WBC1 is the first count value, and WBC2 is the second count value.
8. The cell analyzer for sheep, goats and cows of claim 6, wherein: the analyzer execution body is further configured to be capable of executing a red blood cell count detection item on a blood sample, and comprises a sampling device, a diluent supply device, a hemolysis agent supply device, a white blood cell count cell, a red blood cell count cell, a first detection device for performing white blood cell count detection, and a second detection device for performing red blood cell count detection;
the controller is configured to: when the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer to execute the following actions by the main body:
controlling the sampling device to collect a blood sample and adding the collected blood sample into the white blood cell counting cell;
controlling the diluent supply device to add diluent into the white blood cell counting cell so as to dilute the blood sample to a fourth preset proportion value, and preparing a fourth detection sample liquid;
Controlling the sampling device to collect a first part of the fourth detection sample liquid from the white blood cell counting cell, and controlling the sampling device to add at least part of the fourth detection sample liquid in the collected first part of the fourth detection sample liquid into the red blood cell counting cell for dilution and red blood cell counting detection;
controlling the sampling device to collect a second part of the fourth detection sample liquid from the white blood cell counting cell;
controlling emptying of the fourth detection sample liquid remaining in the white blood cell count cell;
controlling the sampling device to add at least part of the fourth detection sample liquid in the collected second part of the fourth detection sample liquid into the white blood cell counting cell;
controlling the diluent supply device to convey diluent into the white blood cell counting cell, controlling the hemolytic agent supply device to convey hemolytic agent into the white blood cell counting cell so as to dissolve red blood cells in the fourth detection sample liquid, and diluting the blood sample to a third preset proportion value to prepare a third detection sample liquid;
controlling the first detection device to perform white blood cell counting detection on the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells;
Controlling the diluent supply device to convey diluent into the white blood cell counting cell, and controlling the hemolytic agent supply device to convey hemolytic agent into the white blood cell counting cell so as to dilute the third detection sample liquid to the first preset proportion value, so as to prepare the first detection sample liquid;
controlling the first detection device to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data;
wherein the fourth preset proportion value is smaller than the third preset proportion value.
9. A cell detection method suitable for sheep, goats and cows, characterized in that: the method comprises the following steps:
a sampling step of controlling a sampling device to collect a blood sample in a blood sample container;
a first sampling step of controlling the sampling device to convey at least part of the collected blood sample to a white blood cell counting cell;
a first detection sample liquid preparation step, namely controlling a diluent supply device to convey diluent into the white blood cell counting tank, controlling a hemolytic agent supply device to convey a hemolytic agent into the white blood cell counting tank so as to dissolve red blood cells in the blood sample, and diluting the blood sample to a first preset proportion value to prepare a first detection sample liquid;
A first detection step of controlling a first detection device to perform white blood cell count detection on the first detection sample liquid by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of the white blood cells;
wherein the first preset ratio value is greater than or equal to 1200:1 and less than or equal to 3500:1.
10. the method for cell detection in sheep, goats and cows as claimed in claim 9, wherein: the first preset proportion value is greater than or equal to 1500:1 and less than or equal to 3000:1.
11. the method for cell detection in sheep, goats and cows as claimed in claim 10, wherein: the first preset proportion value is (2400+/-200): 1.
12. the method for cell detection in sheep, goats and cows as claimed in claim 9, wherein: the cell detection method further includes a third detection sample liquid preparation step after the first sample separation step and before the first detection sample liquid preparation step, the third detection sample liquid preparation step including: controlling the diluent supply device to convey diluent into the white blood cell counting pool, controlling the hemolytic agent supply device to convey hemolytic agent into the white blood cell counting pool so as to dissolve red blood cells in the blood sample, and diluting the blood sample to a third preset proportion value to prepare a third detection sample liquid;
The first detection sample liquid preparation step comprises the following steps: diluting at least part of the third detection sample liquid to the first preset proportion value by adopting a diluent and a hemolytic agent to prepare the first detection sample liquid;
the cell detection method further comprises a second detection step after the third detection sample liquid preparation step, the second detection step comprising: controlling the first detection device to perform white blood cell count detection on at least part of the third detection sample liquid by adopting an impedance method to obtain second detection data;
wherein the third preset ratio value is greater than or equal to 1200:1 and less than the first preset proportional value.
13. The method for cell detection in sheep, goats and cows as claimed in claim 12, wherein: the cell detection method further comprises a fourth detection sample liquid preparation step before the third detection sample liquid preparation step, wherein the fourth detection sample liquid preparation step comprises the following steps: controlling the diluent supply device to convey diluent into the white blood cell counting cell so as to dilute the blood sample to a fourth preset proportion value, and preparing a fourth detection sample liquid;
the cell detection method further comprises a second sample separation step after the fourth detection sample liquid preparation step, the second sample separation step comprising: delivering a first portion of the fourth test sample fluid to a red blood cell count cell;
The third detection sample liquid preparation step comprises the following steps: dissolving red blood cells in the fourth detection sample liquid of a third part by adopting a diluent and a hemolytic agent, and diluting the fourth detection sample liquid of the third part to a third preset proportion value to prepare the third detection sample liquid;
wherein the fourth preset proportion value is smaller than the third preset proportion value.
14. The method for cell detection in sheep, goats and cows as claimed in claim 13, wherein: the second sampling step comprises the following steps: controlling the sampling device to collect a first part of the fourth detection sample liquid from the white blood cell counting cell, and controlling the sampling device to add the collected first part of the fourth detection sample liquid into the red blood cell counting cell;
the third detection sample liquid preparation step comprises the following steps: controlling the sampling device to collect a second part of the fourth detection sample liquid from the white blood cell counting tank, and controlling to empty the fourth detection sample liquid remained in the white blood cell counting tank; controlling the diluent supply device to convey diluent into the white blood cell counting tank to form a base solution, controlling the sampling device to add a third part of the fourth detection sample liquid into the white blood cell counting tank, controlling the diluent supply device to convey diluent into the white blood cell counting tank, controlling the hemolytic agent supply device to convey hemolytic agent into the white blood cell counting tank so as to dissolve red blood cells in the blood sample, and diluting the blood sample to a third preset proportion value to prepare the third detection sample liquid;
Wherein the fourth detection sample fluid volume of the second portion is greater than or equal to the fourth detection sample fluid volume of the third portion.
15. A cell detection method according to any one of claims 12 to 14, suitable for sheep, goats and cows, wherein:
the first detection data comprises a first histogram and the first count value of white blood cells, wherein the first histogram is a white blood cell number and volume distribution histogram; the second detection data comprises a second histogram and a second count value of white blood cells, wherein the second histogram is a histogram of the number and volume distribution of red blood cell fragments and white blood cells;
the cell detection method further includes a data analysis step including: analyzing the first detection data and the second detection data to obtain the classified count data of the white blood cells;
the data analysis step includes: calculating S1 and S2 according to the formula S1/(s1+s2) =wbc1/wbc2, determining the boundary between red cell fragments and white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram through the dividing line to obtain a third histogram; classifying and counting the white blood cells in the third histogram according to the volume size to obtain classifying and counting data of the white blood cells;
Wherein S1 is the area of the distribution area of the white blood cells in the first histogram, S2 is the area of the distribution area of the red blood cell fragments in the second histogram, WBC1 is the first count value, and WBC2 is the second count value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111017316.8A CN113777009B (en) | 2021-08-31 | 2021-08-31 | Cell analyzer and cell detection method suitable for sheep, goats and cows |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111017316.8A CN113777009B (en) | 2021-08-31 | 2021-08-31 | Cell analyzer and cell detection method suitable for sheep, goats and cows |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113777009A CN113777009A (en) | 2021-12-10 |
| CN113777009B true CN113777009B (en) | 2024-04-16 |
Family
ID=78840512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111017316.8A Active CN113777009B (en) | 2021-08-31 | 2021-08-31 | Cell analyzer and cell detection method suitable for sheep, goats and cows |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113777009B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101470109A (en) * | 2007-12-25 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Method for improving accuracy of blood sample leukocyte classifying result |
| CN112114000A (en) * | 2019-06-19 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyzer, method for classifying leukocytes based on impedance method and computer-readable storage medium |
| WO2020252692A1 (en) * | 2019-06-19 | 2020-12-24 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyzer, impedance method-based white blood cells classification method, and computer-readable storage medium |
| WO2021051349A1 (en) * | 2019-09-19 | 2021-03-25 | 深圳迈瑞生物医疗电子股份有限公司 | Method for analysis of animal blood cells, analyzer, and storage medium |
| WO2021077327A1 (en) * | 2019-10-23 | 2021-04-29 | 深圳迈瑞生物医疗电子股份有限公司 | Method for analyzing red blood cells in blood sample and blood analysis system |
| WO2021147179A1 (en) * | 2020-01-22 | 2021-07-29 | 深圳市锦瑞生物科技有限公司 | Blood particle detection method and blood analyzer thereof |
-
2021
- 2021-08-31 CN CN202111017316.8A patent/CN113777009B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101470109A (en) * | 2007-12-25 | 2009-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Method for improving accuracy of blood sample leukocyte classifying result |
| CN112114000A (en) * | 2019-06-19 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyzer, method for classifying leukocytes based on impedance method and computer-readable storage medium |
| WO2020252692A1 (en) * | 2019-06-19 | 2020-12-24 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyzer, impedance method-based white blood cells classification method, and computer-readable storage medium |
| WO2021051349A1 (en) * | 2019-09-19 | 2021-03-25 | 深圳迈瑞生物医疗电子股份有限公司 | Method for analysis of animal blood cells, analyzer, and storage medium |
| WO2021077327A1 (en) * | 2019-10-23 | 2021-04-29 | 深圳迈瑞生物医疗电子股份有限公司 | Method for analyzing red blood cells in blood sample and blood analysis system |
| WO2021147179A1 (en) * | 2020-01-22 | 2021-07-29 | 深圳市锦瑞生物科技有限公司 | Blood particle detection method and blood analyzer thereof |
Non-Patent Citations (3)
| Title |
|---|
| Label-free whole flood cell differentiation based on multiple frequency AC impedance and light scattering analysis in a micro flow cytometer;Peter Simon et al;《Royal society of Chemistry》;20160513;全文 * |
| Leukocyte analysis and differentation using high speed microfluidic single cell impedance cytometry;David Holmes et al;《Royal Society of Chemistry》;20090807;全文 * |
| 绵羊和水牛实验感染大片吸虫后外周血白细胞计数的分析;张为宇;《广西农业生物科学》;20060930;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113777009A (en) | 2021-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105891090B (en) | Blood cell analyzer, body fluid analysis method, and control system therefor | |
| US20190339258A1 (en) | Sample analyzer and computer program product | |
| EP1953526B1 (en) | Hematological analyzer, method for analyzing body fluid and computer program product | |
| JP4745030B2 (en) | Blood analyzer | |
| JP4994920B2 (en) | Sample analyzer | |
| US6391263B1 (en) | Automated analyzing system and method therefor | |
| JP5771236B2 (en) | Blood analyzer | |
| CN111542744B (en) | Blood analyzer, blood analysis method, and computer-readable storage medium | |
| EP1033573B1 (en) | Automated analyzing system and method thereof | |
| CN105388099B (en) | Urine analyzer and method for analyzing abnormal cells in urine or body fluid | |
| EP2694960A2 (en) | Identifying and enumerating early granulated cells (egcs) | |
| CN109270281A (en) | Improve the method and apparatus of leukocyte differential count result accuracy and count results repeatability | |
| CN107525758B (en) | Method for controlling blood analyzer to determine blood platelet | |
| US9465026B2 (en) | Apparatus for measuring blood cells and immunity from whole blood | |
| CN114174826A (en) | Animal blood cell analysis method, analyzer and storage medium | |
| EP3336557A1 (en) | Analysis apparatus | |
| CN108732338A (en) | A kind of classification of compatible leucocyte five and leucocyte three divide the cellanalyzer and test method of group | |
| CN113777009B (en) | Cell analyzer and cell detection method suitable for sheep, goats and cows | |
| WO2021147179A1 (en) | Blood particle detection method and blood analyzer thereof | |
| CN209911384U (en) | Blood detection device | |
| CN110967322A (en) | Blood cell analyzer and blood cell analyzing method | |
| CN207366563U (en) | A kind of classification of compatible leucocyte five and leucocyte three divide the cellanalyzer of group | |
| CN108303363B (en) | Blood cell analysis method and blood cell analyzer using same | |
| CN212379420U (en) | Sample analyzer | |
| JP2010223833A (en) | Animal blood cell measuring apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 518000 room 702, building 4, Yinxing Zhijie phase III, No. 1301-88, sightseeing Road, Xinlan community, Guanlan street, Longhua District, Shenzhen City, Guangdong Province Applicant after: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Address before: 518000 building 4 (complex building) 512, no.1301-74, sightseeing Road, Xinlan community, Guanlan street, Longhua District, Shenzhen City, Guangdong Province Applicant before: Shenzhen Mindray Animal Medical Technology Co.,Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |