CN212379420U - Sample analyzer - Google Patents

Abstract

The utility model provides a sample analyzer, which comprises a sheath flow impedance detection component, an RBC reaction component, a pipeline component, an optical reaction component, an optical detection component, a sampling component and an injector; the RBC reaction assembly is used for processing a sample to be detected to form a first sample liquid, and the sheath flow impedance detection assembly is used for detecting the first sample liquid; the optical reaction assembly is used for processing a sample to be detected to form a second sample liquid, and the optical detection assembly is used for detecting the second sample liquid; the injector is communicated to the sheath flow impedance detection assembly, the RBC reaction assembly, the optical detection assembly and the sampling assembly through the pipeline assembly and is continuously matched with the sampling assembly, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly and the optical detection assembly to work. So as to reduce the cost and volume of the instrument.

Description

Sample analyzer

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to a sample analyzer.

Background

In the five-classification blood cell analyzer, a liquid path scheme generally comprises a sampling power source, an optical channel sample pushing power source and a sheath flow impedance channel sample pushing power source, and the power sources are typically realized by using an injector.

For sampling injector power sources, there are generally two implementations: the first type comprises a blood separating valve, the blood sampling amount is large, but the quantification and the blood separation are realized by the blood separating valve, so the requirement on the quantification precision of the injector is not high, and the injector with a large measuring range, such as a milliliter-grade injector, is generally adopted in the condition; and secondly, the blood is separated by using an injector, the blood collection amount is small, the quantification and the blood separation are realized by the injector, the requirement on the quantification precision of the injector is high, and the injector with a small measuring range, such as a micro-upgrading injector, is generally adopted in the condition.

For optical channel measurement, the flow rate of the sample flow pushing liquid is very low, the flow rate is required to be stable, and a micro-upgrading injector is generally adopted; however, since the optical channel measures different cells, such as white blood cells, red blood cells, PLT, etc., and the difference between the cell volume and the sample concentration is large, there is a large difference between the sample pushing flow rate and the sample pushing volume (for example, the sample volume to be pushed for white blood cell measurement is large, the sample volume to be pushed for low-value sample measurement or pre-diluted sample measurement is large), so a micro-upgrading syringe with a medium range is generally adopted to take account of the quantification accuracy and the sample pushing volume.

For sheath flow impedance measurement, the flow rate of the sample flow pushing liquid is very low, the flow rate is required to be stable, and a micro-upgrading injector is generally adopted; the channel has single measurement concentration and small measurement volume, and a small-range micro-upgrading injector is generally used.

In the current five-classification blood cell analyzer scheme, due to the limitation of measurement speed or incompatibility of syringe performance, independent syringe assemblies are generally provided according to the requirements of each channel respectively so as to meet the requirements of corresponding module sample pushing. However, the five-classification blood cell analyzer uses a large number of syringes, which causes problems of high cost and large volume of the analyzer, and is not favorable for miniaturization and/or integration design of the analyzer.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is necessary to provide a sample analyzer with reduced cost and volume to solve the problems of high instrument cost and large volume caused by the large number of syringes.

The above purpose is realized by the following technical scheme:

a sample analyzer comprises a sheath flow impedance detection assembly, an RBC reaction assembly, a pipeline assembly, an optical reaction assembly, an optical detection assembly, a sampling assembly and an injector;

the RBC reaction assembly is used for processing a sample to be detected to form a first sample liquid, and the sheath flow impedance detection assembly is used for detecting the first sample liquid; the optical reaction assembly is used for processing a sample to be detected to form a second sample liquid, and the optical detection assembly is used for detecting the second sample liquid;

the injector is communicated to the sheath flow impedance detection assembly, the RBC reaction assembly, the optical detection assembly and the sampling assembly through the pipeline assembly and is continuously matched with the sampling assembly, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly and the optical detection assembly to work.

In one embodiment, the syringe is capable of sampling and separating a sample to be tested during continuous operation of the syringe, and the syringe is further capable of pushing a first sample fluid into the sheath flow impedance detection assembly and a second sample fluid into the optical detection assembly.

In one embodiment, the tube assembly includes a first connection tube, a second connection tube, and a third connection tube, the first connection tube connects the sampling assembly and the injector, one end of the second connection tube is connected to the first connection tube, the other end of the second connection tube is connected to the sheath flow impedance detection assembly, one end of the third connection tube is connected to the first connection tube, and the other end of the third connection tube is connected to the optical detection assembly.

In one embodiment, the pipeline assembly further comprises a first switching piece, and the first switching piece is used for realizing on-off communication between the first connecting pipeline and the second connecting pipeline as well as between the first connecting pipeline and the third connecting pipeline.

In one embodiment, the first switching element is disposed at a connection position of the first connecting pipeline and the second and third connecting pipelines, and the first switching element has three leading-out ends, one of which is connected with the first connecting pipeline, and the other two leading-out ends are respectively connected with the first connecting pipeline and the second connecting pipeline.

In one embodiment, the first connection pipeline has a first access point, and the first access point is respectively connected with the second connection pipeline and a third connection pipeline; the first switching piece comprises a first sub-switching piece and a second sub-switching piece, the first sub-switching piece is arranged on the second connecting pipeline, and the second sub-switching piece is arranged on the third connecting pipeline.

In one embodiment, the sampling assembly comprises a sampling needle, a sampling pipeline and a second switching piece, the sampling pipeline is connected with the sampling needle and the second switching piece, the first connecting pipeline is connected with the second switching piece and the injector, and the injector and the second switching piece are matched for sampling and sample dividing of a sample to be detected.

In one embodiment, the first connection pipeline further has a second access point, the second access point is connected with the sampling pipeline, and the second switching piece is disposed on the sampling pipeline.

In one embodiment, the second switching member is disposed at a connection of the sampling pipe and the first connecting pipe.

In one embodiment, the second connecting line has a third access point, and the second connecting line connects the sheath flow impedance detecting assembly and the RBC reaction assembly through the third access point respectively.

In one embodiment, the RBC reaction assembly includes an RBC reaction tank, the pipeline assembly further includes an RBC sample preparation pipeline and a third switching piece, the RBC reaction tank is used for processing a sample to be tested to form a first sample liquid, the RBC sample preparation pipeline connects the third access point and the RBC reaction tank, and the third switching piece is disposed in the RBC sample preparation pipeline.

In one embodiment, the third connecting line has a fifth access point, and the third connecting line connects the optical detection assembly and the optical reaction assembly through the fifth access point.

In one embodiment, the optically reactive component comprises an optically reactive cell; the pipeline assembly further comprises an optical sample preparation pipeline and a fifth switching piece, the optical reaction tank is used for processing a sample to be detected to form a second sample liquid, the optical sample preparation pipeline is connected with the fifth access point and the optical reaction tank, and the fifth switching piece is arranged on the optical sample preparation pipeline.

In one embodiment, the range of the syringe is 100uL to 300 uL.

After the technical scheme is adopted, the utility model discloses following technological effect has at least:

the utility model discloses a sample analyzer, a syringe pass through pipeline subassembly and connect sampling subassembly, RBC reaction unit and sheath flow impedance detection subassembly, and the syringe cooperates sampling subassembly, RBC reaction unit and sheath flow impedance detection subassembly in the measurement process in succession, realizes multiplexing of syringe, and effectual solution syringe is the instrument that leads to in large quantity with high costs, bulky problem, reduces the cost and the volume of instrument by a wide margin, does benefit to the miniaturized and/or design that integrates of instrument.

Drawings

Fig. 1 is a liquid path connection diagram of a sample analyzer according to a first embodiment of the present invention;

fig. 2 is a liquid path connection diagram of a sample analyzer according to a second embodiment of the present invention;

fig. 3 is a liquid path connection diagram of a sample analyzer according to a third embodiment of the present invention;

fig. 4 is a liquid path connection diagram of a sample analyzer according to a fourth embodiment of the present invention;

fig. 5 is a liquid path connection diagram of a sample analyzer according to a fifth embodiment of the present invention;

fig. 6 is a schematic view of the measurement cycle of the sample analyzer of the present invention.

Wherein: 100. a sample analyzer; 110. an injector; 120. a sampling component; 121. a sampling needle; 122. a sampling pipeline; 123. a second switching member; 130. a tubing assembly; 131. a first connecting line; s1, the first access point; 132. a second connecting line; s3, a third access point; 133. a third connecting pipeline; s5, a fifth access point; 134. a first switching member; 1341. a first sub-switch; 1342. a second sub-switch; 135. RBC sample preparation line; 136. a third switching member; 137. an optical sample preparation line; 138. a fifth switching member; 140. an RBC reaction tank; 150. a sheath flow impedance detection assembly; 151. a sheath flow impedance detection unit; 152. a sheath flow impedance detection sample needle; 153. preparing a power source for a sheath flow impedance detection sample; 154. a sheath flow impedance detection sample preparation pipeline; 1541. a first liquid preparation section; 1542. the first negative pressure power source is connected with the pipeline; 155. a sheath fluid chamber; 156. a fourth switching member; s4, a fourth access point; 160. an optical reaction cell; 170. an optical detection assembly; 171. an optical detection unit; 172. optically inspecting the sample needle; 173. optically detecting a sample preparation power source; 174. an optical detection pipeline; 1741. a second liquid preparation section; 1742. the second negative pressure power source is connected with the pipeline; 175. a sixth switching member; s6, a sixth access point; 180. a diluent assembly; 181. a diluent compartment; 182. a diluent line; 183. and a seventh switching member.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1-6, the present invention provides a sample analyzer 100. The sample analyzer 100 is used for analyzing and detecting a sample to be detected to obtain a corresponding detection result, and meets the use requirement. It should be noted that the specific type of sample to be tested is not limited, and in some embodiments, the sample to be tested includes a solid sample or a liquid sample. It can be understood that the liquid sample needs to be placed on the sample holder for detection. Further liquid samples include, but are not limited to, blood samples.

The utility model discloses in use the sample that awaits measuring to explain as the blood sample. Specifically, when the sample analyzer 100 detects a sample to be measured, which is a blood sample, the sample to be measured is stored in a test tube and sequentially placed on a test tube rack. The utility model discloses a sample analyzer 100 is suitable for under laboratory environmental condition, accomplishes the in vitro diagnostic equipment of measurement such as blood cell count, five categorizations of leucocyte, hemoglobin concentration measurement, the measurement of reticulocyte, nucleated red blood cell measurement.

The utility model discloses a sample analyzer 100 can realize the continuous detection of the multi-parameter of the sample that awaits measuring, under the prerequisite of guaranteeing that detection efficiency satisfies the operation requirement, can reduce the volume of instrument, simultaneously, reduces the cost of instrument, does benefit to sample analyzer 100 miniaturization/design of integrating.

Referring to fig. 1-5, in one embodiment, the sample analyzer 100 includes a sheath flow impedance detection assembly 150, a Red Blood Cell (RBC) reaction assembly, a tubing assembly 130, an optical reaction assembly, an optical detection assembly 170, a sampling assembly 120, and an injector 110.

The sampling assembly 120 is used for collecting a sample to be detected, the RBC reaction assembly is used for processing the sample to be detected to form a first sample liquid, and the sheath flow impedance detection assembly 150 is used for detecting the first sample liquid; the optical reaction assembly is used for processing the sample to be detected to form a second sample solution, and the optical detection assembly 170 is used for detecting the second sample solution. The injector 110 is connected to the sheath flow impedance detection assembly 150, the RBC reaction assembly, the optical detection assembly 170 and the sampling assembly 120 through the tubing assembly 130, and is continuously operated in cooperation with the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly 150 and the optical detection assembly 170.

The sample analyzer 100 has a syringe 110, and the sheath flow impedance detection module 150, the RBC reaction module, the tubing module 130, the optical reaction module, the optical detection module 170, and the sampling module 120 are connected to each other through the syringe 110. The injector 110 can be respectively matched with the sheath flow impedance detection assembly 150, the RBC reaction assembly, the pipeline assembly 130, the optical reaction assembly, the optical detection assembly 170, and the sampling assembly 120, so as to realize the analysis and detection operation of the sample analyzer 100 on the sample to be detected.

After the injector 110 is connected to the sampling assembly 120, the sampling assembly 120 may be controlled to suck the sample to be tested by the operation of withdrawing the injector 110, so that the sample to be tested is stored in the sampling assembly 120, i.e., the sample sucking function of the sampling assembly 120 is realized. Specifically, the sample analyzer 100 has a sampling position, and after the sample to be tested is conveyed to the sampling position of the sample analyzer 100, the sampling assembly 120 can suck the sample to be tested at the sampling position, thereby completing the sampling operation of the sampling assembly 120. It will be appreciated that the sample to be tested may be transported to the sampling location by a healthcare worker directly moving the test tube of the sample to be tested to the sampling location, or the test tube with the sample to be tested may be transported by a sample transport assembly of the sample analyzer 100, which may be of the current blood analyzer configuration.

The sampling assembly 120 can also move to the RBC reaction assembly to deliver the sample to be tested to the RBC reaction assembly, and move to the optical reaction assembly to deliver the sample to be tested to the RBC reaction assembly and the optical reaction assembly, respectively, i.e., the sampling assembly 120 can sample. The sample separating function of the sampling assembly 120 is controlled by the injector 110, and the pushing operation of the injector 110 can control the sample to be detected in the sampling assembly 120 to be output from the sampling assembly 120, so as to realize sample separation of the sample to be detected. The injector 110 can ensure the quantitative accuracy of sample division, so that the sample to be detected which is divided into the RBC reaction component and the optical reaction component can meet the actual detection requirement, and simultaneously, the waste is avoided.

The sample analyzer 100 further has a master controller and a sampling driving part, the sampling driving part is connected with the sampling assembly 120, and the sampling driving part can drive the sampling assembly 120 to move so as to move the sampling assembly 120 at the sampling position, the RBC reaction assembly and the optical reaction assembly. Specifically, the master controller can control the sampling driving part to drive the sampling assembly 120 to move to the sampling position, and control the sampling assembly 120 to pierce into the test tube; the main controller can also control the sampling driving part to drive the sampling assembly 120 to move to the RBC reaction assembly; the master controller can also control the sampling driving part to drive the sampling assembly 120 to move to the optical reaction assembly.

In principle, the order of the RBC reaction module and the optical reaction module in the sampling module 120 is not limited, and the RBC reaction module may be first sampled, or the optical reaction module may be first sampled. Illustratively, the sampling assembly 120 moves to the RBC reaction assembly for sample separation and then moves to the optical reaction assembly for sample separation. Since the sample to be measured needs to be incubated in the RBC reaction module after the sample is separated from the RBC reaction module by the sampling module 120, the incubation needs a certain time. The sample separation from the RBC reaction module by the sampling module 120 is controlled, and the sample separation from the optical reaction module by the sampling module 120 can be controlled during the incubation process of the sample to be detected, so that the waiting time of the injector 110 can be shortened, and the processing efficiency of the sample analyzer 100 can be improved.

The cooperation of the RBC reaction assembly and the sheath flow impedance detection assembly 150 can realize sheath flow impedance method detection of the sample to be detected. Specifically, the RBC reaction assembly receives a sample to be detected, which is divided by the sampling assembly 120, and can incubate the sample to be detected to obtain a first sample solution for red blood cell detection. The sheath flow impedance detection assembly 150 uses negative pressure to draw the first sample liquid out of the RBC reaction assembly, the syringe 110 pushes the first sample liquid into the sheath flow impedance detection assembly 150, and the sheath flow impedance detection assembly 150 performs sheath flow impedance detection on the sample to be detected, so as to obtain the red blood cell parameters and the platelet parameters.

The optical reaction component and the optical detection component 170 cooperate to perform optical detection on the sample to be detected. Specifically, the optical reaction component receives the sample to be detected, which is divided by the sampling component 120, and can perform incubation processing on the sample to be detected, so as to obtain a second sample liquid for detecting the leukocyte parameter. The optical detection module 170 uses negative pressure to draw the second sample solution out of the optical reaction module, and the injector 110 pushes the second sample solution into the optical detection module 170, and the optical detection module 170 performs optical detection on the sample to be detected to obtain leukocyte parameters, such as white blood cell count or white blood cell classification.

The sample analyzer 100 further includes an injection driving part electrically connected to the main controller, and the injection driving part may drive the syringe 110 to move so that the syringe 110 performs a pushing or sucking operation. When the master controller controls the injection driving part to drive the injector 110 to perform a suction operation, the injector 110 can enable the sampling assembly 120 to suck samples; when the master controller controls the injection driver to drive the injector 110 to perform a pushing operation, the injector 110 may sample the sampling assembly 120, or push the first sample fluid into the sheath flow impedance detection assembly 150 and the second sample fluid into the optical detection assembly 170. Alternatively, the injection driving part is a stepping motor or other power source capable of ensuring quantitative accuracy.

The sample analyzer 100 of the present invention works by continuously matching the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly 150 and the optical detection assembly 170 with one syringe 110. One measurement cycle of the sample analyzer 100 is: the sampling assembly 120 moves to the sampling position, and the injector 110 controls the sampling assembly 120 to suck the sample; then, the sampling assembly 120 moves to the RBC reaction assembly, the injector 110 controls the sampling assembly 120 to sample the RBC reaction assembly, and the RBC reaction assembly processes the sample to be detected to form a first sample solution; the sampling assembly 120 moves to the optical reaction assembly, the injector 110 controls the sampling assembly 120 to sample the optical reaction assembly, and the optical reaction assembly processes the sample to be measured to form a second sample solution. Then, the first sample liquid in the RBC reaction module flows out, the syringe 110 pushes the first sample liquid to the sheath flow impedance detection module 150, the second sample liquid in the optical reaction module flows out, and the syringe 110 pushes the second sample liquid to the optical detection module 170, thereby completing the detection of a sample to be detected.

The continuous fitting here means that the fitting of the syringe 110 to each component is continuous in one measurement cycle, as shown in fig. 6, that is, the operations of sucking, separating, and pushing the sample performed by the syringe 110 in the measurement cycle are continuous and sequential, and after the sample sucking operation is completed by the syringe 110, the sample separating operation is performed, and then the sample pushing operation is performed. It will be appreciated that, because the movement of the sampling assembly 120 and the transfer of the sample fluid take some time, the syringe 110 remains inactive during this process; the injector 110 is activated when the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly 150, and the optical detection assembly 170 need to be operated.

It should be noted that, when the number of samples to be measured is one, the sample analyzer 100 is stopped after the sample analyzer 100 detects one sample to be measured. When the number of samples to be measured is plural, plural measurement cycles of the sample analyzer 100 are continuously performed.

In the sample analyzer 100 of the above embodiment, one injector 110 is used to connect the sampling assembly 120, the RBC reaction assembly and the sheath flow impedance detection assembly 150 through the pipeline assembly 130, and the injector 110 is continuously matched with the sampling assembly 120, the RBC reaction assembly and the sheath flow impedance detection assembly 150 in the measurement process, so that multiplexing of the injector 110 is realized, the problems of high instrument cost and large volume caused by a large number of injectors 110 are effectively solved, the instrument cost and volume are greatly reduced, and the instrument miniaturization and/or integration design is facilitated.

It should be noted that the sample analyzer 100 of the present invention is not limited to sheath flow impedance detection and optical detection of the sample to be measured, and can also perform other measurements on the sample to be measured. At this time, a corresponding detecting component and a reaction component matching with the detecting component are added to the sample analyzer 100, and the sampling component 120 can also sample into the reaction component and match with the detecting component for detection by the injector 110.

In one embodiment, the sample analyzer 100 further includes a reagent supply assembly. The reagent supply assembly supplies the processing reagent to the RBC reaction assembly and the optical reaction assembly, so that the blood sample to be tested is mixed with the processing reagent supplied by the reagent supply assembly to prepare a sample liquid. Specifically, the reagent supply assembly provides a processing reagent for the RBC reaction assembly and the optical reaction assembly respectively, a sample to be detected in the RBC reaction assembly and the processing reagent are incubated and reacted to form a first sample solution, and a sample to be detected in the optical reaction assembly and the processing reagent are incubated and reacted to form a second sample solution.

In some embodiments, the reagent supply assembly includes a first reagent supply that supplies a red blood cell reagent, such as a diluent or the like. In some embodiments, the reagent supply assembly comprises a second reagent supply for supplying a leukocyte reagent, for example comprising a haemolysing agent capable of lysing red blood cells in the blood sample, optionally also comprising a fluorescent reagent capable of staining white blood cells, or the like. Of course, in other embodiments, the reagent supply assembly includes a third reagent supply for supplying a hemoglobin reagent, such as a hemolysing agent capable of lysing red blood cells in a blood sample, releasing hemoglobin from the red blood cells, and converting hemoglobin to methemoglobin. In some embodiments, the leukocyte reagent and the hemoglobin reagent are the same hemolytic agent, i.e., the second reagent supply and the third reagent supply are the same reagent supply.

Referring to fig. 1-5, in one embodiment, during continuous operation of the syringe 110, the syringe 110 can sample and sort the sample to be tested, and the syringe 110 can also push a first sample fluid into the sheath flow impedance detection assembly 150 and a second sample fluid into the optical detection assembly 170. That is, in one cycle of the injector 110, the injector 110 works in continuous cooperation with the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly 150, and the optical detection assembly 170, so that the injector 110 is multiplexed, and the injector 110 has multiple functions, so as to reduce the number of the injectors 110, and further reduce the volume and the cost of the sample analyzer 100.

Specifically, the injector 110 controls the sampling assembly 120 to suck the sample to be tested to sample the sample to be tested, and the injector 110 controls the sampling assembly 120 to discharge the sample to be tested to sample the sample to be tested. After the sheath flow impedance detection assembly 150 sucks the first sample liquid out of the RBC reaction assembly, the syringe 110 pushes the first sample liquid into the sheath flow impedance detection assembly 150, and after the optical detection assembly 170 sucks the second sample liquid out of the optical reaction assembly, the syringe 110 pushes the second sample liquid into the optical detection assembly 170, so that sample pushing of the first sample liquid and the second sample liquid is realized. That is, the sample sucking, sample separating and sample pushing functions are realized by one injector 110, so as to greatly reduce the cost and volume of the instrument.

Referring to fig. 1 to 5, in an embodiment, the tubing assembly 130 includes a first connecting tubing 131, a second connecting tubing 132, and a third connecting tubing 133, the first connecting tubing 131 connects the sampling assembly 120 and the syringe 110, one end of the second connecting tubing 132 is connected to the first connecting tubing 131, the other end of the second connecting tubing 132 is connected to the sheath flow impedance detecting assembly 150, one end of the third connecting tubing 133 is connected to the first connecting tubing 131, and the other end of the third connecting tubing 133 is connected to the optical detecting assembly 170.

The first connecting line 131, the second connecting line 132, and the third connecting line 133 form a Y-shaped passage, one end of the first connecting line 131 is connected to the syringe 110, and the other end of the first connecting line 131 is connected to the second connecting line 132 and the third connecting line 133, respectively. The sampling assembly 120 is connected to a first connecting line 131. The end of the second connecting line 132 away from the first connecting line 131 connects the RBC reaction module with the sheath flow impedance detection module 150, and the end of the third connecting line 133 away from the first connecting line 131 connects the optical reaction module with the optical detection module 170.

The first connecting line 131, the second connecting line 132 and the third connecting line 133 are used to establish the communication relationship between the syringe 110 and the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly 150 and the optical detection assembly 170. Thus, the injector 110 is respectively matched with each module through the pipelines, and the functions of sample sucking, sample separating and sample pushing of one injector 110 are realized.

Referring to fig. 1 to 5, in an embodiment, the pipeline assembly 130 further includes a first switching element 134, and the first switching element 134 enables on-off communication between the first connecting pipeline 131 and the second and third connecting pipelines 132 and 133. The first switching element 134 is used for controlling the on-off relationship between the first connecting pipeline 131 and the second and third connecting pipelines 132 and 133. Optionally, the first switch 134 is a valve or other component capable of on-off control. The first switching member 134 can switch the communication relationship between the first connecting line 131, select the on/off of the first connecting line 131 and the second connecting line 132, and select the on/off of the first connecting line 131 and the third connecting line 133.

It will be appreciated that in order to ensure that the syringe 110 is operating correctly, only one of the paths is open and the other path is open. Illustratively, when the injector 110 performs sample sucking and separating operations, the first switching element 134 controls the first connecting pipeline 131 and the second connecting pipeline 132 to be both open-circuited, and at this time, the injector 110 is communicated with the sampling assembly 120, so as to realize sampling of a sample to be measured. When the syringe 110 performs a sample pushing operation and pushes the first sample liquid, the first switch 134 controls the first connection pipeline 131 to communicate with the second connection pipeline 132, and controls the first connection pipeline 131 to form an open circuit with the third connection pipeline 133. When the syringe 110 performs a sample pushing operation and pushes the second sample liquid, the second switch 123 controls the first connecting pipeline 131 to communicate with the third connecting pipeline 133, and controls the first connecting pipeline 131 to form an open circuit with the second connecting pipeline 132.

Referring to fig. 1 and 5, in an embodiment, a first switching element 134 is disposed at a connection portion of the first connection pipeline 131, the second connection pipeline 132 and the third connection pipeline 133, the first switching element 134 has three leading-out terminals, one of the leading-out terminals is connected to the first connection pipeline 131, and the other two leading-out terminals are respectively connected to the first connection pipeline 131 and the second connection pipeline 132. That is, the first switch 134 is disposed at the connection portion of the first connection pipe 131, the second connection pipe 132 and the third connection pipe 133, that is, the first switch 134 is disposed at the branch of the Y-shape. Thus, one end of the first switching member 134 is connected to the end of the first pipe line remote from the syringe 110, and the two lead-out ends of the first switching member 134 are connected to the ends of the second and third connection pipe lines 132 and 133, respectively. At this time, the first switching element 134 may control on/off of the first connection pipe 131 and the second connection pipe 132, and may also control on/off of the first connection pipe 131 and the third connection pipe 133.

Illustratively, the first switch 134 is a three-way valve. Three leading-out ends of the first switching piece 134 are three ports of a three-way valve, and the three leading-out ends are respectively connected to the first connecting pipeline 131, the second connecting pipeline 132 and the third connecting pipeline 133, so that on-off control of the first connecting pipeline 131, the second connecting pipeline 132 and the third connecting pipeline 133 is realized. The first switching element 134 is electrically connected to the master controller, and the master controller controls the on/off relationship of the first switching element 134 to the first connecting pipeline 131, the second connecting pipeline 132 and the third connecting pipeline 133.

Referring to fig. 2 to 3, in an embodiment, the first connection pipe 131 has a first connection point S1, and the first connection point S1 connects the second connection pipe 132 and the third connection pipe 133, respectively; the first switching member 134 includes a first sub-switching member 1341 and a second sub-switching member 1342, the first sub-switching member 1341 is disposed on the second connecting pipe 132, and the second sub-switching member 1342 is disposed on the third connecting pipe 133. Optionally, the first access point S1 is a tee or a component with three interfaces. The communication relationship among the first connecting line 131, the second connecting line 132, and the third connecting line 133 is established through the first access point S1. After the first connection point S1 is connected to the first connection pipe 131, the second connection pipe 132 and the third connection pipe 133 can be connected to the first connection pipe 131 through the first connection point S1.

After the first connection pipeline 131, the second connection pipeline 132 and the third connection pipeline 133 are connected through the first access point S1, the first connection pipeline 131, the second connection pipeline 132 and the third connection pipeline 133 are always in a passage. In order to control the on/off of the first connecting pipeline 131, the second connecting pipeline 132 and the third connecting pipeline 133, the first switching member 134 includes a first sub-switching member 1341 and a second sub-switching member 1342, the first sub-switching member 1341 is disposed on the second connecting pipeline 132, and the second sub-connecting member is disposed on the third connecting pipeline 133. The on/off of the second connection pipe 132 is controlled by the first sub-switch 1341, and the on/off of the third connection pipe 133 is controlled by the second sub-switch 1342.

Exemplarily, the first sub-switch 1341 and the second sub-switch 1342 are two-way valves. Further, the first sub-switching member 1341 is a normally open two-way valve or a normally closed two-way valve, and the second sub-switching member 1342 is a normally open two-way valve or a normally closed two-way valve. Of course, in other embodiments of the present invention, the first sub-switch 1341 and the second sub-switch 1342 may also be three-way valves or other valves capable of being switched on and off.

Referring to fig. 1 to 5, in an embodiment, the sampling assembly 120 includes a sampling needle 121, a sampling pipe 122 and a second switching member 123, the sampling pipe 122 connects the sampling needle 121 and the second switching member 123, the first connecting pipe 131 connects the second switching member 123 and the injector 110, and the injector 110 and the second switching member 123 cooperate to realize sampling and sample division of a sample to be measured. The sampling needle 121 can penetrate into the test tube to suck the sample to be tested. The sampling needle 121 is connected to the first connecting pipeline 131 through a lighting pipeline, so as to be connected to the syringe 110, and the second switching member 123 is used for controlling the on-off of the sampling pipeline 122. Alternatively, the second switching member 123 is a valve or other component capable of performing on-off control to switch the communication relationship between the sampling pipe 122 and the first connecting pipe 131.

Specifically, when the sample analyzer 100 needs to take a sample, the second switch 123 controls the sampling line 122 to communicate with the first connection line 131, so that the sampling needle 121 communicates with the syringe 110. At this time, the injector 110 can control the sampling needle 121 to suck the sample to be tested in the test tube, thereby completing the sample sucking operation of the sample to be tested. When the sample analyzer 100 requires sample division, the second switch 123 controls the sampling line 122 to communicate with the first connection line 131 so that the sampling needle 121 communicates with the syringe 110. At this time, the injector 110 can control the sampling needle 121 to discharge the sample to be tested in the test tube, thereby completing the sample splitting operation of the sample to be tested. When the sample analyzer 100 performs a sample pushing operation or other operations, the second switch 123 opens the sampling assembly 120, and the syringe 110 cannot perform a sample sucking operation or a sample separating operation.

Also, the movement of the sampling needle 121 is driven by the sampling driving part. Specifically, the sampling needle 121 is connected to the sampling driving unit, and the sampling driving unit controls the sampling needle 121 to move, so that the sampling needle 121 moves between the sampling position, the RBC reaction component and the optical reaction component, and controls the sampling needle 121 to move up and down, thereby realizing the sample suction and sample separation of the sampling needle 121.

Referring to fig. 3 to 5, in an embodiment, the first connection pipeline 131 further has a second access point, the second access point is connected to the sampling pipeline 122, and the second switch 123 is disposed on the sampling pipeline 122. Optionally, the first access point S1 is a tee or a component with three interfaces. Two of the three interfaces of the second access point are connected to the first connection line 131, so that the first connection line 131 forms a passage, while the third interface of the second connection point is connected to the sampling line 122.

The provision of the second access point corresponds to the tapping of a branch on the first connecting line 131, which branch enables the first connecting line 131 to communicate with the sampling line 122. Thus, the second switching member 123 may be directly provided on the sampling pipe 122. The second switching member 123 is disposed behind the sampling pipe 122, and can directly control the on/off of the sampling pipe 122. When the second switch 123 controls the sampling pipe 122 to be a passage, the sampling pipe 122 can realize the communication between the sampling needle 121 and the injector 110; when the second switch 123 controls the sampling pipe 122 to be disconnected, the sampling pipe 122 cannot communicate the sampling needle 121 with the syringe 110.

Optionally, the second switch 123 is a two-way valve. Further, the second switching member 123 is a normally open two-way valve or a normally closed two-way valve.

Referring to fig. 1 and 2, in an embodiment, the second switching member 123 is disposed at a connection of the sampling line 122 and the first connection line 131. That is, the second switch 123 is located at the branch between the sampling line 122 and the first connection line 131. At this time, the second switching member 123 has three terminals, two of the terminals are connected to the first connection pipe 131, so that the first connection pipe 131 forms a passage, and the third terminal of the second switching member 123 extends out of the sampling pipe 122. The second switching member 123 establishes a communication relationship between the sampling pipeline 122 and the first connecting pipeline 131 to control the connection and disconnection of the first connecting pipeline 131 and the sampling pipeline 122. Exemplarily, the second switching member 123 is a three-way valve.

By the cooperation of the first switching member 134 and the second switching member 123, the syringe 110 can be connected to only one component through the first connecting line 131. The first and second switches 134 and 123 may be a combination of three-way valves and/or two-way valves.

In the first embodiment of the present invention, as shown in fig. 1, the first switching element 134 and the second switching element 123 are three-way valves, the second switching element 123 is disposed between the first connecting pipeline 131 and the sampling pipeline 122, and the first switching element 134 connects the first connecting pipeline 131 and the second connecting pipeline 132 through three leading-out terminals.

Specifically, when the sample analyzer 100 sucks or divides a sample, the main controller controls the second switch 123 to connect the sampling pipe 122 and the first connecting pipe 131, and controls the first connecting pipe 131 and the second switch 123 to form an open circuit, at this time, the syringe 110, the second connecting pipe 132 and the third connecting pipe 133 are open circuits, and the syringe 110 is connected to the sampling needle 121. The injector 110 can control the sampling needle 121 to perform a sample sucking or separating operation of a sample to be measured. When the sample analyzer 100 performs a sample pushing operation, the master controls the second switch 123 to connect the first connecting pipeline 131 and the second switch 123, and disconnect the sampling pipeline 122 and the first connecting pipeline 131. At this time, the main controller may control the second connection pipe 132 or the third connection pipe 133 to communicate with the first connection pipe 131 through the first switching member 134, so as to enable the syringe 110 to perform a sample pushing operation.

In the second embodiment of the present invention, referring to fig. 2, the first switch 134 includes a first sub-switch 1341 and a second sub-switch 1342, the first sub-switch 1341 and the second sub-switch 1342 are two-way valves, the second switch 123 is a three-way valve, the second switch 123 is disposed between the first connecting pipeline 131 and the sampling pipeline 122, the second connecting pipeline 132 and the third connecting pipeline 133 are inserted into the first connecting pipeline 131 through the first access point S1, the first sub-switch 1341 is disposed on the second connecting pipeline 132, and the second sub-switch 1342 is disposed on the third connecting pipeline 133.

Specifically, when the sample analyzer 100 sucks or divides a sample, the main controller controls the second switch 123 to connect the sampling pipe 122 and the first connecting pipe 131, and controls the first connecting pipe 131 and the second switch 123 to form an open circuit, at this time, the syringe 110, the second connecting pipe 132 and the third connecting pipe 133 are open circuits, and the syringe 110 is connected to the sampling needle 121. The injector 110 can control the sampling needle 121 to perform a sample sucking or separating operation of a sample to be measured. When the sample analyzer 100 performs a sample pushing operation, the master controls the second switch 123 to connect the first connecting pipeline 131 and the second switch 123, and disconnect the sampling pipeline 122 and the first connecting pipeline 131. At this time, the main controller may control the second connection pipeline 132 or the third connection pipeline 133 to communicate with the first connection pipeline 131 through the first sub-switch 1341 or the second sub-switch 1342, so that the injector 110 performs a sample pushing operation.

In the third embodiment of the present invention, the rest is the same as the second embodiment except that the setting position and the type of the second switching member 123 are changed. Referring to fig. 3, the second switching member 123 is a two-way valve, the sampling pipeline 122 is connected to the first connecting pipeline 131 through a second access point, and the second switching member 123 is disposed on the sampling pipeline 122 and directly controls on/off of the sampling pipeline 122. The second switching member 123 is a normally closed two-way valve.

Specifically, when the sample analyzer 100 sucks or divides a sample, the main controller controls the second switch 123 to connect the sampling pipe 122 and the first connecting pipe 131, and controls the first sub-switch 1341 and the second sub-switch 1342 to disconnect the first connecting pipe 131 from the second connecting pipe 132 and the third connecting pipe 133, at this time, the syringe 110 is disconnected from the second connecting pipe 132 and the third connecting pipe 133, and the syringe 110 is connected to the sampling needle 121. The injector 110 can control the sampling needle 121 to perform a sample sucking or separating operation of a sample to be measured. When the sample analyzer 100 performs a sample pushing operation, the second switch 123 does not act, and the sampling pipeline 122 can be directly disconnected, and at this time, the main controller can control the second connecting pipeline 132 or the third connecting pipeline 133 to communicate with the first connecting pipeline 131 through the first sub-switch 1341 or the second sub-switch 1342, so that the injector 110 performs the sample pushing operation.

In the fourth embodiment of the present invention, referring to fig. 4, except that the second switching member 123 is a normally open two-way valve, the connection relationship and the working mode of the sample analyzer 100 are the same as those of the third embodiment, which are not repeated herein.

In the fifth embodiment of the present invention, the rest is the same as the first embodiment except that the setting position and the type of the second switching member 123 are changed. Referring to fig. 5, the second switching member 123 is a two-way valve, the sampling pipeline 122 is connected to the first connection pipeline 131 through a second access point, the second switching member 123 is disposed on the sampling pipeline 122 to directly control the connection and disconnection of the sampling pipeline 122, and the first switching member 134 is a three-way valve.

Specifically, when the sample analyzer 100 sucks or divides a sample, the main controller controls the second switching element 123 to connect the sampling pipeline 122 and the first connecting pipeline 131, and controls the first switching element 134 to disconnect the first connecting pipeline 131 from the second connecting pipeline 132 and the third connecting pipeline 133, at this time, the syringe 110 is disconnected from the second connecting pipeline 132 and the third connecting pipeline 133, and the syringe 110 is connected to the sampling needle 121. When the sample analyzer 100 performs a sample pushing operation, the master controller controls the second switch 123 to disconnect the first connecting pipeline 131 from the sampling pipeline 122, and at this time, the master controller can control the second connecting pipeline 132 or the third connecting pipeline 133 to communicate with the first connecting pipeline 131 through the first switch 134, so that the injector 110 performs the sample pushing operation.

It should be noted that, in the above embodiment, when the second switch 123 adopts a three-way valve, the sampling component 120 samples, and the second connecting pipeline 132 or the third connecting pipeline 133 which is communicated with the normally open end of the second switch 123 is required to be a closed pipeline, so as to avoid interfering with sampling.

Referring to fig. 1 to 5, in an embodiment, the second connecting line 132 has a third access point S3, and the second connecting line 132 connects the sheath flow impedance detecting assembly 150 and the RBC reaction assembly through the third access point S3. Optionally, the third access point S3 is a tee or a component with three interfaces. The second connecting line 132 is connected to the RBC reaction module and the sheath flow impedance detecting module 150 through the third access point S3. The third access point S3 has three ports, one of which is connected to the second connecting line 132, and the other two of which are connected to the RBC reaction module and the sheath flow impedance detection module 150, respectively.

The third connection point is equivalent to two branched branches formed on the second connection pipeline 132, so that the second connection pipeline 132 forms a Y-shaped passage, and the communication relationship between the second connection pipeline 132 and the RBC reaction assembly and the sheath flow impedance detection assembly 150 is realized. After the RBC reaction assembly incubates the sample to be detected to form the first sample liquid, the sheath flow impedance detection assembly 150 may suck the first sample liquid out of the RBC reaction assembly by using negative pressure, and then communicate with the injector 110 through the second connecting pipeline 132, so that the injector 110 quantitatively pushes the first sample liquid into the sheath flow impedance detection assembly 150, thereby realizing detection of the sample to be detected.

In an embodiment, the RBC reaction assembly includes an RBC reaction tank 140, the pipeline assembly 130 further includes an RBC sample preparation pipeline 135 and a third switching element 136, the RBC reaction tank 140 is configured to process a sample to be tested to form a first sample liquid, the RBC sample preparation pipeline 135 connects the third access point S3 and the RBC reaction tank 140, and the third switching element 136 is disposed in the RBC sample preparation pipeline 135. The RBC reaction cell 140 is used for incubating a sample to be tested, so that the sample to be tested forms a first sample solution. Specifically, the first reagent supply unit is communicated with the RBC reaction tank 140, and is configured to deliver a processing reagent to the RBC reaction tank 140, so that the processing reagent processes and incubates the sample to be tested in the RBC reaction tank 140 to form a first sample solution. Optionally, the third switch 136 is a valve or other component capable of on-off control.

When the sample to be tested is incubated in the RBC reaction cell 140, the third switching element 136 is in a disconnected state, and the RBC sample preparation line 135, the second connecting line 132, and the sheath flow impedance detection assembly 150 are all open circuits. After the incubation of the sample to be detected in the RBC reaction tank 140 is completed, the third switching element 136 is opened, the RBC sample preparation pipeline 135, the second connecting pipeline 132 and the sheath flow impedance detection assembly 150 are used as a passage, and the first sample liquid in the RBC reaction tank 140 can be output. At this time, the sheath flow impedance detecting assembly 150 outputs negative pressure to suck the first sample liquid to be separated from the RBC sample preparation pipeline 135, but the first sample liquid cannot directly enter the sheath flow impedance detecting assembly 150 for detection, which requires the second connecting pipeline 132 to be communicated with the injector 110, and the injector 110 pushes the first sample liquid to enter the sheath flow impedance detecting assembly 150 to realize detection of the sample to be detected.

It is understood that the sheath flow impedance detecting assembly 150 has a first liquid preparation section 1541, the first liquid preparation section 1541 connects the sheath flow impedance detecting assembly 150 with the third access point S3, the first sample liquid sucked by the sheath flow impedance detecting assembly 150 is stored in the first liquid preparation section 1541, but cannot enter the sheath flow impedance detecting assembly 150, and the injector 110 provides power to push the first sample liquid into the sheath flow impedance detecting assembly 150. In addition, the syringe 110 can control the volume of the first sample liquid for sheath flow impedance detection, so as to ensure the quantitative volume of the detection and the accuracy of the detection.

The sheath flow impedance detecting unit 150 is used to detect a first sample liquid prepared from a portion of a sample to be measured and a red blood cell reagent supplied from a first reagent supplying portion to obtain a red blood cell parameter and a platelet parameter. In one embodiment, the sheath flow impedance detecting assembly 150 includes a sheath flow impedance detection sample preparation power source 153, a sheath flow impedance detecting section 151, a sheath flow impedance detection sample needle 152, a sheath flow impedance detection sample preparation line 154, and a fourth switch 156, the sheath flow impedance detection sample preparation line 154 has a fourth access point S4, the sheath flow impedance detection sample preparation line 154 is connected to the sheath flow impedance detection sample preparation power source 153 and is accessed to the third access point S3 to connect to the RBC sample preparation line 135, the sheath flow impedance detection sample needle 152 is connected to the fourth access point S4 and is located in the sheath flow impedance detecting section 151, and the sheath flow impedance detecting section 151 is connected to the sheath fluid tank 155. The sheath flow impedance detection sample preparation power source 153 sucks the first sample liquid in the RBC reaction cell 140 into the sheath flow impedance detection sample preparation line 154 and the sheath flow impedance detection sample needle 152, and the syringe 110 pushes the first sample liquid into the sheath flow impedance detection unit 151.

The sheath flow impedance detecting unit 151 has a detection hole with an electrode, and the sheath flow impedance detecting unit 151 further has an outlet for outputting the first sample liquid after detection. The sheath flow impedance detecting unit 151 detects a dc impedance generated when the particle in the first sample liquid passes through the detection hole, and outputs an electric signal reflecting information when the particle passes through the hole. Illustratively, the sheath flow impedance detecting unit 151 is a sheath flow impedance counting cell. The sheath flow impedance detection sample preparation line 154 is used to deliver the first sample liquid to the sheath flow impedance detection section 151. The sheath fluid tank 155 is connected to the sheath flow impedance detecting unit 151, and supplies the sheath fluid to the sheath flow impedance detecting unit 151.

The sheath flow impedance detection sample preparation pipeline 154 includes two sections, one section is a first liquid preparation section 1541, the other section is a first negative pressure power source connecting pipeline 1542, one end of the first liquid preparation section 1541 is connected to the third access point S3, and the other end of the first liquid preparation section 1541 is respectively connected to the sheath flow impedance detection sample needle 152 and the first negative pressure power source connecting pipeline 1542 through the fourth access point S4. The end of the sheath flow impedance detection sample needle 152 projects into the sheath flow impedance detection unit 151. The end of the first negative pressure power source connecting line 1542 remote from the fourth access point S4 is connected to the sheath flow impedance detection sample preparation power source 153. The sheath flow impedance test sample preparation power source 153 may generate a negative pressure to draw the first sample fluid from the RBC reaction well 140. The fourth switching member 156 is disposed on the first negative pressure power source connecting pipe 1542, and is configured to control on/off of the first negative pressure power source connecting pipe 1542.

After the incubation of the sample to be detected in the RBC reaction tank 140 is completed to form the first sample liquid, the main controller controls the sheath flow impedance detection sample preparation power source 153 to prepare, and controls the third switching element 136 and the fourth switching element 156 to open, so that the first negative pressure power source connecting pipeline 1542, the first liquid preparation section 1541 and the RBC sample preparation pipeline 135 are communicated. At this time, the sheath flow impedance detection sample preparation power source 153 generates a negative pressure, and sucks the first sample liquid in the RBC reaction cell 140 into the first sample liquid preparation section 1541 at the fourth access point S4, that is, at the inlet of the sheath flow impedance detection unit 151. Subsequently, the master controller controls the first switch 134 and the second switch 123 to operate, so that the second connecting pipeline 132 is communicated to the syringe 110 through the first connecting pipeline 131, the syringe 110 starts to push the sample, and the first sample liquid in the first preparation liquid section 1541 is injected into the sheath flow impedance detecting portion 151 through the sheath flow impedance detecting sample needle 152.

When the sheath flow impedance detection sample needle 152 ejects the first sample liquid in the sheath flow impedance detection section 151, the first sample liquid flows under the sheath liquid, the detection hole changes the first sample liquid flow into a thin flow, and the particles (formed components) contained in the first sample liquid pass through the detection hole one by one. The electrodes are electrically connected to a dc power supply that provides a dc current between a pair of electrodes. The impedance between the pair of electrodes can be detected while the dc power is supplied from the dc power supply. The resistance signal representing the impedance change is amplified by the amplifier and then transmitted to the main controller. The size of the resistance signal corresponds to the volume (size) of the particles, so that the red blood cell parameter and the platelet parameter of the sample liquid to be detected can be obtained by signal processing of the resistance signal through the main controller.

It is worth explaining, sheath flow impedance detection portion 151 is the sheath flow impedance counting cell promptly, and its concrete structure and detection principle are prior art, the utility model discloses in for the convenience understanding only describe the partial technique that sheath flow impedance detection portion 151 detected.

Referring to fig. 1 to 5, in an embodiment, the third connecting line 133 has a fifth connecting point S5, and the third connecting line 133 connects the optical detection module 170 and the optical reaction module through the fifth connecting point S5. Optionally, the fifth access point S5 is a tee or a component with three interfaces. The communication relationship between the third connecting pipeline 133 and the optical reaction assembly and the optical detection assembly 170 is established through the fifth access point S5. The fifth access point S5 has three ports, one of which is connected to the third connecting pipe 133, and the other two of which are connected to the optical reaction module and the optical detection module 170, respectively.

The third connection point is equivalent to two branches formed on the third connection pipeline 133, so that the third connection pipeline 133 forms a Y-shaped passage, and the third connection pipeline 133 is communicated with the optical reaction assembly and the optical detection assembly 170. After the optical reaction assembly incubates the sample to be detected to form a second sample solution, the optical detection assembly 170 may suck the second sample solution out of the optical reaction assembly by using a negative pressure, and then communicate with the injector 110 through the third connecting pipeline 133, so that the injector 110 quantitatively pushes the second sample solution into the optical detection assembly 170, thereby detecting the sample to be detected.

In one embodiment, the optical reaction assembly includes an optical reaction cell 160; the tube assembly 130 further includes an optical sample preparation tube 137 and a fifth switch 138, the optical reaction cell 160 is used for processing the sample to be tested to form a second sample solution, the optical sample preparation tube 137 connects the fifth access point S5 and the optical reaction cell 160, and the fifth switch 138 is disposed on the optical sample preparation tube 137. The optical reaction cell 160 is used for incubating the sample to be tested, so that the sample to be tested forms a second sample solution. Specifically, the second reagent supply portion is communicated with the optical reaction cell 160, and is used for delivering a processing reagent to the optical reaction cell 160, so that the processing reagent processes and incubates the sample to be measured in the optical reaction cell 160 to form a second sample solution. Optionally, the fifth switch 138 is a valve or other component capable of performing on-off control.

When the sample to be tested is incubated in the optical reaction cell 160, the fifth switching element 138 is in the off state, and the optical sample preparation pipeline 137, the third connecting pipeline 133 and the optical detection assembly 170 are all in the off state. After the incubation of the sample to be detected in the optical reaction cell 160 is completed, the fifth switch 138 is opened, the optical sample preparation pipeline 137, the third connecting pipeline 133 and the optical detection assembly 170 are used as passages, and the second sample liquid in the optical reaction cell 160 can be output. At this time, the optical detection assembly 170 outputs negative pressure to suck the second sample liquid to the separation optical sample preparation pipeline 137, but the second sample liquid cannot directly enter the optical detection assembly 170 for detection, which requires the third connecting pipeline 133 to communicate with the injector 110, and the injector 110 pushes the second sample liquid to enter the optical detection assembly 170 to realize the detection of the sample to be detected.

It is understood that the optical detection assembly 170 has a second liquid preparation segment 1741, the second liquid preparation segment 1741 connects the optical detection assembly 170 with the fifth access point S5, and the second sample liquid sucked out by the optical detection assembly 170 is stored in the second liquid preparation segment 1741 but cannot enter the optical detection assembly 170, and the injector 110 provides power to push the second sample liquid into the optical detection assembly 170. In addition, the injector 110 can control the volume of the second sample liquid for optical detection, so as to ensure the quantitative volume of the detection and the accuracy of the detection.

The optical detection unit 170 is used to detect a second sample solution prepared from a portion of the sample to be measured and the leukocyte reagent supplied from the second reagent supply portion to obtain an optical parameter of the sample to be measured. Illustratively, the optical detection component can detect WBCs (white blood cells), NRBCs (nuclear red blood cells), RETs (reticulocytes), BRCs, PLTs, and the like of a sample to be detected.

In one embodiment, the optical inspection assembly 170 includes an optical inspection sample preparation power source 173, an optical inspection section 171, an optical inspection sample needle 172, an optical inspection pipe 174, and a sixth switching member 175, the optical inspection pipe 174 has a sixth access point S6, the optical inspection pipe 174 is connected to the optical inspection sample preparation power source 173 and is accessed to the fifth access point S5 to connect to the optical sample preparation pipe 137, and the optical inspection sample needle 172 is connected to the sixth access point S6 and extends into the optical inspection section 171. The optical detection sample preparation power source 173 sucks the second sample solution in the optical reaction cell 160 into the optical detection line 174 and the optical detection sample needle 172, and the syringe 110 pushes the second sample solution into the optical detection unit 171.

The optical detection pipeline 174 includes two segments, one segment is a second liquid preparation segment 1741, the other segment is a second negative pressure power source connecting pipeline 1742, one end of the second liquid preparation segment 1741 is connected to the fifth access point S5, and the other end of the second liquid preparation segment 1741 is respectively connected to the optical detection sample needle 172 and the second negative pressure power source connecting pipeline 1742 through the sixth access point S6. The end of the optical detection sample needle 172 projects into the optical detection section 171. An end of the second negative pressure power source connecting tube 1742 remote from the sixth access point S6 is connected to the optical test sample preparation power source 173. The optical detection sample preparation power source 173 may generate a negative pressure to draw the second sample liquid in the optical reaction cell 160. The sixth switching member 175 is disposed on the second negative pressure power source connecting pipe 1742, and is configured to control on/off of the second negative pressure power source connecting pipe 1742.

After the incubation of the sample to be detected in the optical reaction cell 160 is completed to form the second sample liquid, the main controller controls the preparation of the optical detection sample preparation power source 173 and controls the opening of the fifth switching element 138 and the sixth switching element 175, so that the second negative pressure power source connecting pipeline 1742, the second liquid preparation segment 1741 and the optical sample preparation pipeline 137 are communicated. At this time, the optical detection sample preparation power source 173 generates a negative pressure, and the second sample liquid in the optical reaction cell 160 is drawn into the second sample liquid preparation section 1741 at the sixth access point S6, that is, the inlet of the optical detection unit 171. Subsequently, the master controller controls the first switch 134 and the second switch 123 to operate, so that the third connecting pipeline 133 is communicated to the syringe 110 through the first connecting pipeline 131, the syringe 110 starts to push the sample, and the second sample liquid in the second liquid preparation segment 1741 is injected into the optical detection portion 171 through the optical detection sample needle 172.

The optical detection section 171 has a light source, a beam shaping section, a flow cell, and a forward scatter detector, which are arranged in this order on a straight line. On one side of the flow cell, a dichroic mirror is arranged at an angle of 45 ° to the straight line. After the second sample liquid is ejected by the optical detection sample needle 172, a part of the side light emitted by the blood cells in the flow cell is transmitted through the dichroic mirror and captured by the fluorescent light detector arranged behind the dichroic mirror at an angle of 45 ° to the dichroic mirror, while another part of the side light is reflected by the dichroic mirror and captured by the side scattered light detector arranged in front of the dichroic mirror at an angle of 45 ° to the dichroic mirror. From the forward scattered light signals captured by the forward scatter light detector, the side scattered light signals captured by the side scatter light detector and the fluorescence signals captured by the fluorescence detector, leukocytes in the blood sample can be counted and classified, for example, into at least neutrophils, lymphocytes and monocytes.

It should be noted that the optical detection unit 171 is an optical flow cell, and the specific structure and the detection principle thereof are prior art, and only a part of the technology detected by the optical detection unit 171 is described in the present invention for convenience of understanding.

In one embodiment, the range of the syringe 110 is 100uL to 300 uL. That is, the syringe 110 of the present invention has a large range. Thus, the requirements of quantitative accuracy and quantitative volume of each module are met, the reuse of the injector 110 is realized, and the operation of matching one injector 110 with each module is realized. Preferably, the range of the syringe 110 is 200uL to 300 uL. Further, the range of the syringe 110 is 250 uL. In addition, in order to improve the quantitative precision of the injector 110, the injection driving part is adopted to realize the precise control of the injector 110, and the accuracy of the detection result is ensured. Optionally, the injection drive is a stepper motor.

Referring to fig. 1-5, in one embodiment, the sample analyzer 100 further includes a diluent assembly 180, the diluent assembly 180 being coupled to the syringe 110. The diluent assembly 180 is used to provide diluent to each module of the sample analyzer 100, for example, when the RBC reaction assembly needs diluent, the injector 110 can control to push the diluent in the diluent assembly 180 into the RBC reaction assembly; when cleaning is required, the syringe 110 pushes the diluent in the diluent block 180 into the component to be cleaned for cleaning.

In one embodiment, the diluent assembly 180 includes a diluent pipe 182, a diluent tank 181, and a seventh switch 183, wherein the diluent pipe 182 connects the syringe 110 and the diluent tank 181, and the seventh switch 183 is disposed on the diluent pipe 182. The seventh switch 183 is a valve or other component capable of on-off control. The seventh switching element 183 controls the on/off of the diluent pipe 182, so as to control the on/off of the syringe 110 and the diluent tank 181. When the diluent is required to be used, the seventh switching member 183 controls the diluent line 182 to be opened to communicate the syringe 110 with the diluent tank 181. When diluent is not available, the seventh switch 183 controls the diluent line 182 to be disconnected to disconnect the syringe 110 from the diluent tank 181.

The working process of the sample analyzer 100 of the present invention is described by the arrangement of the switching element in the first embodiment, and the working principle of the switching element in other embodiments is substantially the same as that of the first embodiment, which is not repeated herein. Referring to fig. 1, the sample analyzer 100 of the first embodiment operates as follows:

when the sampling assembly 120 samples, the sampling assembly 120 is first controlled to move to the sampling position, the main controller controls the second switching element 123 to connect the sampling pipeline 122 and the first connecting pipeline 131, so that the sampling needle 121 is connected to the injector 110, and meanwhile, the main controller controls the first switching element 134 to disconnect the first connecting pipeline 131 and the second switching element 123, so that the second switching element 123 disconnects the connection relationship between the first connecting pipeline 131 and the second connecting pipeline 132 as well as the third connecting pipeline 133. At this time, the master controller controls the injector 110 to drive the sampling needle 121 to sample. After sampling is completed, the main controller controls the sampling assembly 120 to move to the RBC reaction assembly, samples are separated from the RBC reaction tank 140, the RBC reaction tank 140 incubates a sample to be detected to form a first sample solution, in the incubation process, the main controller controls the sampling assembly 120 to move to the optical reaction assembly, samples are separated from the optical reaction tank 160, and the optical reaction tank 160 incubates the sample to be detected to form a second sample solution. The master controller controls the second switch 123 to close the sampling pipe 122.

After the sampling of the sampling assembly 120 is completed, the sheath flow impedance detecting assembly 150 performs a sample preparation operation, the sheath flow impedance detecting sample preparation power source 153 operates, and the main controller controls the third switching element 136 and the fourth switching element 156 to open. The negative pressure of the sheath flow impedance detection sample preparation power source 153 sucks the first sample liquid in the RBC reaction cell 140 into the first sample liquid preparation section 1541 and the first sample liquid into the fourth access point S4, so that the first sample liquid is located at the inlet of the sheath flow impedance detection unit 151. Then, the sheath flow impedance detecting assembly 150 is ready to measure, and the main controller controls the first switch 134 to communicate the second switch 123 with the first connecting pipe 131, and controls the second switch 123 to open the second connecting pipe 132, so that the first switch 134 and the second switch 123 communicate the first connecting pipe 131 with the second connecting pipe 132, and the syringe 110 is conducted with the sheath flow impedance detecting unit 151. The syringe 110 starts pushing the sample to the sheath flow resistance detecting unit 151, and the sheath flow resistance detecting unit 151 detects the first sample liquid.

After the injector 110 finishes pushing the first sample liquid, the optical detection module 170 performs a sample preparation operation, the optical detection sample preparation power source 173 works, and the main controller controls the fifth switching element 138 and the sixth switching element 175 to open. The negative pressure of the optical detection sample preparation power source 173 sucks the second sample liquid in the optical reaction cell 160 into the second sample liquid preparation stage 1741, and sucks the second sample liquid into the sixth access point S6 so that the second sample liquid is located at the inlet of the optical detection unit 171. Then, the optical detection module 170 prepares for measurement, and the master controller controls the first switch 134 to communicate the second switch 123 with the first connection pipe 131, and controls the second switch 123 to open the third connection pipe 133, so that the first switch 134 and the second switch 123 communicate the first connection pipe 131 with the third connection pipe 133, and the injector 110 is conducted with the optical detection unit 171. The syringe 110 starts to push the sample toward the optical detection unit 171, and the optical detection unit 171 detects the second sample liquid.

After the optical detection and the sheath flow impedance detection are completed, the main controller controls the seventh switching part 183 to be opened, and each part is cleaned by using diluent, so that cross infection is avoided. After the cleaning is completed, the master controller controls the syringe 110 to reset. At this point, the sample analyzer 100 stops operating or performs the next measurement cycle.

Referring to fig. 1 to 5, the present invention further provides a detection method of the sample analyzer 100, which includes the following steps:

controlling the sampling assembly 120 to move to the position above the sample to be detected, and controlling the injector 110 to drive the sampling assembly 120 to collect the sample to be detected;

controlling the sampling assembly 120 to move to the RBC reaction assembly, controlling the injector 110 to drive the sampling assembly 120 to convey the sample to be detected to the RBC reaction assembly, and processing the sample to be detected by the RBC reaction assembly to form a first sample liquid;

controlling the sampling assembly 120 to move to the optical reaction assembly, controlling the injector 110 to drive the sampling assembly 120 to convey the sample to be detected to the optical reaction assembly, and processing the sample to be detected by the optical reaction assembly to form a second sample liquid;

flowing the first sample fluid in the RBC reaction assembly and pushing the first sample fluid by the syringe 110 into the sheath flow impedance detection assembly 150;

flowing the second sample liquid in the optical reaction assembly and pushing the second sample liquid into the optical detection assembly 170 by the injector 110;

the syringe 110 operates continuously with the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance sensing assembly 150, and the optical sensing assembly 170 during a measurement cycle.

The sample analyzer 100 of the present invention works by continuously cooperating the sampling assembly 120, the RBC reaction assembly, the rigid chemical reaction assembly, the sheath flow impedance detection assembly 150 and the optical detection assembly 170 with one syringe 110. One measurement cycle of the sample analyzer 100 is: the sampling assembly 120 moves to the sampling position, and the injector 110 controls the sampling assembly 120 to suck the sample; then, the sampling assembly 120 moves to the RBC reaction assembly, the injector 110 controls the sampling assembly 120 to sample the RBC reaction assembly, and the RBC reaction assembly processes the sample to be detected to form a first sample solution; the sampling assembly 120 moves to the optical reaction assembly, the injector 110 controls the sampling assembly 120 to sample the optical reaction assembly, and the optical reaction assembly processes the sample to be measured to form a second sample solution. Then, the first sample liquid in the RBC reaction module flows out, the syringe 110 pushes the first sample liquid to the sheath flow impedance detection module 150, the second sample liquid in the optical reaction module flows out, and the syringe 110 pushes the second sample liquid to the optical detection module 170, thereby completing the detection of a sample to be detected.

The injector 110 is continuously matched with each component in a measuring period, that is, the sample sucking, sample separating and sample pushing actions of the injector 110 are continuously and sequentially performed, and after the sample sucking operation of the injector 110 is completed, the sample separating operation is respectively performed, and then the sample pushing operation is respectively performed. It will be appreciated that, because the movement of the sampling assembly 120 and the transfer of the sample fluid take some time, the syringe 110 remains inactive during this process; the injector 110 is activated when the sampling assembly 120, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly 150, and the optical detection assembly 170 need to be operated.

In the above description, although it is possible to describe each element of the present invention using expressions such as "first" and "second", they are not intended to limit the corresponding elements. For example, the above expressions are not intended to limit the order or importance of the corresponding elements. The above expressions are used to distinguish one element from another.

The technical features of the embodiments described above can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims (14)

1. A sample analyzer is characterized by comprising a sheath flow impedance detection assembly, an RBC reaction assembly, a pipeline assembly, an optical reaction assembly, an optical detection assembly, a sampling assembly and an injector;

the RBC reaction assembly is used for processing a sample to be detected to form a first sample liquid, and the sheath flow impedance detection assembly is used for detecting the first sample liquid; the optical reaction assembly is used for processing a sample to be detected to form a second sample liquid, and the optical detection assembly is used for detecting the second sample liquid;

the injector is communicated to the sheath flow impedance detection assembly, the RBC reaction assembly, the optical detection assembly and the sampling assembly through the pipeline assembly and is continuously matched with the sampling assembly, the RBC reaction assembly, the optical reaction assembly, the sheath flow impedance detection assembly and the optical detection assembly to work.

2. The sample analyzer of claim 1, wherein the syringe is capable of sampling and dispensing a sample to be tested during continuous operation of the syringe, and wherein the syringe is further capable of advancing a first sample fluid into the sheath flow impedance detection assembly and a second sample fluid into the optical detection assembly.

3. The sample analyzer of claim 2, wherein the tubing assembly includes a first connecting tubing, a second connecting tubing, and a third connecting tubing, the first connecting tubing connects the sampling assembly and the syringe, one end of the second connecting tubing is connected to the first connecting tubing, the other end of the second connecting tubing is connected to the sheath flow impedance detection assembly, one end of the third connecting tubing is connected to the first connecting tubing, and the other end of the third connecting tubing is connected to the optical detection assembly.

4. The sample analyzer of claim 3, wherein the tubing assembly further comprises a first switch that enables on-off communication of the first connecting tubing with the second connecting tubing and the third connecting tubing.

5. The sample analyzer as claimed in claim 4, wherein the first switch is disposed at the connection of the first connecting pipeline and the second and third connecting pipelines, and the first switch has three leading terminals, one of which is connected to the first connecting pipeline, and the other two of which are respectively connected to the first connecting pipeline and the second connecting pipeline.

6. The sample analyzer of claim 4, wherein the first connecting line has a first access point that connects the second connecting line and a third connecting line, respectively; the first switching piece comprises a first sub-switching piece and a second sub-switching piece, the first sub-switching piece is arranged on the second connecting pipeline, and the second sub-switching piece is arranged on the third connecting pipeline.

7. The sample analyzer of any one of claims 3 to 6, wherein the sampling assembly comprises a sampling needle, a sampling pipeline and a second switch, the sampling pipeline connects the sampling needle and the second switch, the first connecting pipeline connects the second switch and the injector, and the injector and the second switch cooperate to realize sampling and sample separation of a sample to be measured.

8. The sample analyzer of claim 7, wherein the first connection line further has a second access point, the second access point being connected to the sampling line, and the second switch is disposed on the sampling line.

9. The sample analyzer of claim 7, wherein the second switch is disposed at a connection of the sampling tube and the first connecting tube.

10. The sample analyzer of any of claims 3 to 6, wherein the second connecting line has a third access point through which the second connecting line connects the sheath flow impedance detection assembly and the RBC reaction assembly, respectively.

11. The sample analyzer of claim 10, wherein the RBC reaction assembly comprises a RBC reaction cell, the pipeline assembly further comprises a RBC sample preparation pipeline and a third switch, the RBC reaction cell is configured to process a sample to be tested to form a first sample liquid, the RBC sample preparation pipeline connects the third access point and the RBC reaction cell, and the third switch is disposed in the RBC sample preparation pipeline.

12. The sample analyzer of any of claims 3 to 6, wherein the third connecting line has a fifth access point, and the third connecting line connects the optical detection assembly and the optical reaction assembly through the fifth access point.

13. The sample analyzer of claim 12 wherein the optically reactive component comprises an optically reactive cell; the pipeline assembly further comprises an optical sample preparation pipeline and a fifth switching piece, the optical reaction tank is used for processing a sample to be detected to form a second sample liquid, the optical sample preparation pipeline is connected with the fifth access point and the optical reaction tank, and the fifth switching piece is arranged on the optical sample preparation pipeline.

14. The sample analyzer of any of claims 1 to 6 wherein the range of syringe ranges from 100uL to 300 uL.

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