CN111886489B - Sample analyzer and sample analysis method - Google Patents
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- 238000012284 sample analysis method Methods 0.000 title claims description 19
- 210000004369 blood Anatomy 0.000 claims abstract description 668
- 239000008280 blood Substances 0.000 claims abstract description 668
- 238000001514 detection method Methods 0.000 claims abstract description 374
- 238000004062 sedimentation Methods 0.000 claims abstract description 147
- 238000000926 separation method Methods 0.000 claims abstract description 135
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 66
- 238000012360 testing method Methods 0.000 claims abstract description 61
- 238000005070 sampling Methods 0.000 claims abstract description 24
- 238000009826 distribution Methods 0.000 claims abstract description 21
- 238000010521 absorption reaction Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 126
- 239000012530 fluid Substances 0.000 claims description 31
- 210000004027 cell Anatomy 0.000 claims description 30
- 230000003287 optical effect Effects 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 18
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- 230000002776 aggregation Effects 0.000 claims description 11
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
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Abstract
A sample analyzer, comprising: the sampling distribution module (10) comprises a sampler (11), a blood separation valve (12) and a first power device (13), wherein the sampler (11) collects blood samples, the first power device (13) draws the blood samples collected by the sampler (11) into the blood separation valve (11), and the blood separation valve (11) distributes the blood samples into a first part of blood samples and a second part of blood samples; a blood sedimentation detection module (20) for providing a detection place for the first part of blood sample, irradiating light and detecting the absorption or scattering degree of the first part of blood sample to obtain the erythrocyte sedimentation rate of the first part of blood sample; a blood routine testing module (30) for performing blood routine testing on the second portion of the blood sample. Because different parts of the same blood sample are respectively distributed to the blood sedimentation detection module (20) and the blood routine detection module (30), the blood sedimentation detection module (20) and the blood routine detection module (30) can respectively and independently detect in parallel, the overall detection time is short, and the detection efficiency is improved.
Description
Technical Field
The application relates to the field of medical equipment, in particular to a sample analyzer and a sample analysis method.
Background
In the blood in the body, the erythrocytes are in a dispersed suspension state due to the flow of blood and the mutual repulsion of the negative charges on the surface of the erythrocytes. While the isolated blood is allowed to stand, the red blood cells will sink due to gravity. When in pathological state, the type and content of protein in blood plasma will change, which will change the balance of electric charges in blood, reduce the negative charges on the surface of red blood cells, and further make the red blood cells form rouleaux to accelerate sedimentation. Thus, the assessment of the condition can be aided by detecting the rate at which the erythrocytes settle within 1 hour, i.e. the erythrocyte sedimentation rate (Erythrocyte sedimentation rate, ESR).
Although ESR is not diagnostically sensitive and specific, it is still considered a reliable, indirect acute phase inflammatory response factor. ESR is widely used for the treatment and monitoring of infectious diseases, acute and chronic inflammations, rheumatism, connective tissue diseases, cancers, hodgkin's disease and other diseases.
Blood routine is also an indispensable test index in the application scenario of clinical tests. Therefore, there is a need for an all-in-one machine that can detect both ESR and blood routine, thereby meeting the clinical needs for blood testing.
The integrated machine is generally formed by connecting an ESR detection module and a blood routine detection module in series through a pipeline, wherein a blood sample firstly flows through the ESR detection module, and after the ESR is detected, the blood sample flows to the blood routine detection module again to detect blood routine. However, the integrated machine can detect blood conventionally only after the ESR detection is completed, and the detection speed is low.
Disclosure of Invention
In order to solve the above-mentioned problems, an embodiment of the present application provides a sample analyzer, including:
A sample distribution module comprising a sampler for collecting a blood sample, a blood separation valve connected to the sampler by a front line and to the first power device by a rear line, and a first power device for driving the sampler to collect a blood sample and drawing the collected blood sample into the blood separation valve by the front line, wherein the blood separation valve is for separating the blood sample into a first portion of blood sample and a second portion of blood sample;
a blood sedimentation detection module comprising a detection line connected to the blood separation valve and adapted to provide a detection site for a first portion of the blood sample dispensed by the blood separation valve, and an optical detection device adapted to illuminate the first portion of the blood sample in the detection line and to detect the extent of absorption or scattering of light by the first portion of the blood sample in the detection line to obtain a erythrocyte sedimentation rate of the first portion of the blood sample;
A blood routine testing module comprising a blood routine testing reservoir connected to the blood separation valve and configured to provide a testing site for a second portion of the blood sample dispensed by the blood separation valve, and a blood routine testing device configured to perform a blood routine test on the second portion of the blood sample in the blood routine testing reservoir.
In one embodiment, the time at which the blood sedimentation detection module detects the erythrocyte sedimentation rate of the first portion of the blood sample overlaps with the time at which the blood routine detection module detects the blood routine parameter of the second portion of the blood sample.
In one embodiment, the blood separation valve comprises:
The liquid inlet is connected with the sampler through the front pipeline, and the first power device drives the blood sample collected by the sampler to flow into the blood separating valve through the liquid inlet;
The first liquid separation port is connected with the detection pipeline and is used for enabling the first part of blood sample distributed by the blood separation valve to flow into the detection pipeline;
A second liquid separation port connected with the blood routine detection tank, wherein the second liquid separation port is used for allowing the second part of blood sample distributed by the blood separation valve to flow into the blood routine detection tank;
The liquid outlet is connected with the first power device through the rear pipeline, and the first power device drives the blood sample in the blood separating valve to flow from the liquid outlet to the rear pipeline.
In one embodiment, the blood sedimentation detection module further comprises a blood sedimentation detection tank independent of the blood routine detection tank, the detection line being connected to the blood separation valve by the blood sedimentation detection tank for receiving the first portion of the blood sample dispensed by the blood separation valve.
In one embodiment, the blood sedimentation detection module further comprises a second power device connected to the detection line, the second power device being configured to drive the first portion of the blood sample dispensed by the blood separation valve to flow into the detection line and stop and hold the first portion of the blood sample after flowing to a detection zone within the detection line, so that the optical detection device detects the erythrocyte sedimentation rate of the first portion of the blood sample.
In one embodiment, a portion of the back line is the detection line, wherein the first motive device is configured to draw the blood sample collected by the sampler into the blood separation valve until a portion of the blood sample flows into the detection line of the back line.
In one embodiment, the optical detection device comprises:
a light emitter located on a side of a detection zone of the detection circuit and adapted to illuminate the first portion of the blood sample within the detection zone,
And the light receiver is positioned at one side of the detection area of the detection pipeline and is used for detecting the variation of the light emitted by the light emitter after the first part of blood sample is irradiated.
In one embodiment, the test line is horizontally disposed.
In one embodiment, the sample analyzer further comprises a fluid path support module for providing fluid path support for the sample distribution module, the blood sedimentation detection module, and the blood routine detection module.
An embodiment of the present application further provides a sample analysis method, including:
a sampling and distributing step: the first power device drives the sampler to collect a blood sample and draws the blood sample in the sampler into the blood separating valve through a front pipeline which connects the sampler with the blood separating valve, so that the blood sample is divided into a first part of blood sample and a second part of blood sample and is respectively distributed to a blood sedimentation detection module and a blood routine detection module;
And detecting the erythrocyte sedimentation rate: the blood sedimentation detection module irradiates the first part of blood sample and detects the absorption or scattering degree of the first part of blood sample to obtain the erythrocyte sedimentation rate of the first part of blood sample;
Blood routine detection: the blood routine detection device detects blood routine parameters of the second portion of the blood sample.
In one embodiment, the time at which the blood sedimentation detection module detects the erythrocyte sedimentation rate of the first portion of the blood sample overlaps with the time at which the blood routine detection module detects the blood routine parameter of the second portion of the blood sample.
In one embodiment, the sample distribution step includes:
The blood separation valve distributes the first part of blood sample to a detection pipeline of the blood sedimentation detection module or a blood sedimentation detection pond connected with the detection pipeline through a first sample transmission channel of the blood separation valve;
At the same time, the blood separation valve distributes the second portion of the blood sample to a blood routine detection cell of the blood routine detection module through a second sample transmission channel thereof.
In one embodiment, the sample distribution step includes:
The first power device draws the blood sample in the sampler into a blood separating valve through a front pipeline until a part of the blood sample flows into a rear pipeline connecting the first power device and the blood separating valve, wherein the blood sample in the rear pipeline is the first part of blood sample;
The blood separation valve then distributes the second portion of the blood sample to a blood routine testing reservoir of the blood routine testing module via its sample transfer channel.
In one embodiment, the time at which the blood sedimentation detection module initiates detection of the erythrocyte sedimentation rate of the first portion of the blood sample is the same as or different from the time at which the blood routine detection module initiates detection of the blood routine parameter of the second portion of the blood sample.
In one embodiment, the blood sedimentation detection module is started to detect the erythrocyte sedimentation rate of the first part of the blood sample after the blood routine detection module is started to detect the blood routine parameters of the second part of the blood sample.
In one embodiment, the sample analysis method is applied to the sample analyzer of any of the embodiments described above.
The application has the beneficial effects that:
The sample analyzer provided by the embodiment of the application comprises a blood separating valve, wherein the blood separating valve is connected with a sampler through a front pipeline and is connected with a first power device through a rear pipeline. The blood separating valve is further connected with the detection pipeline and the blood conventional detection tank respectively, so that after the blood sample is collected by the sampler, the blood separating valve can distribute the blood sample into a first part of blood sample and a second part of blood sample, the first part of blood sample is distributed to the detection pipeline, the second part of blood sample is distributed to the blood conventional detection tank, the blood sedimentation detection module and the blood conventional detection module can respectively and independently detect blood cell sedimentation rate and blood conventional detection parameters in parallel, the detection of the next detection module can be carried out without waiting for the detection of the last detection module, the overall detection time is short, and the detection efficiency is high.
Drawings
The advantages of the foregoing and/or additional aspects of the present application will become apparent and readily appreciated from the description of the embodiments, taken in conjunction with the accompanying drawings, wherein:
FIG. 1 is a schematic diagram of a sample analyzer according to an embodiment of the present application;
FIG. 2 is a schematic view of the blood separation valve shown in FIG. 1 in another operating condition;
FIG. 3 is a schematic illustration of the connection of the blood separation valve of FIG. 2 with the sampler and the first power device in this operational state;
FIG. 4 is a timing diagram illustrating operation of a sample analyzer according to an embodiment of the present application;
FIG. 5 is a schematic diagram of a sample analyzer according to an embodiment of the present application;
FIG. 6 is a timing diagram illustrating operation of a sample analyzer according to an embodiment of the present application;
FIG. 7 is a schematic diagram of a sample analyzer according to an embodiment of the present application;
FIG. 8 is a schematic diagram of a sample analyzer according to an embodiment of the present application;
Fig. 9 is a flow chart of a sample analysis method according to an embodiment of the application.
Wherein the correspondence between the reference numerals and the component names in fig. 1 to 8 is:
10. a sampling distribution module; 11. a sampler; 111. a sampling needle; 12. a blood separating valve; 121. a liquid inlet; 122. a liquid outlet; 123. an outer sheet; 1231. a liquid inlet channel; 1232. a liquid outlet channel; 1233. a first outer liquid separation channel; 1234. a second external liquid separation channel; 124. middle piece; 1241. a first intermediate liquid separation channel; 1242. a second intermediate liquid separation channel; 125. an inner sheet; 1251. a connection channel; 1252. a first internal fluid distribution channel; 1253. a second internal liquid distribution channel; 13. a first power unit; 14. a front pipeline; 15. a rear pipeline; 20. a blood sedimentation detection module; 21. detecting a pipeline; 22. an optical detection device; 221. a light emitter; 222. an optical receiver; 23. a blood sedimentation detection pool; 24. a second power device; 30. a blood routine detection module; 31. a blood routine detection cell; 40. a liquid path support module; 50. and a control module.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Fig. 1 is a schematic structural view of a sample analyzer according to an embodiment of the present application, fig. 2 is a schematic structural view of the blood separation valve shown in fig. 1 in another working state, and fig. 3 is a schematic connecting view of the blood separation valve shown in fig. 2 with a sampler and a first power device in the working state.
As shown in fig. 1 to 3, an embodiment of the present application provides a sample analyzer including a sample distribution module 10, a blood sedimentation detection module 20, and a blood routine detection module 30.
The sample distribution module 10 comprises a sampler 11, a blood separation valve 12 and a first power means 13. The sampler 11 is used for collecting a blood sample, and the blood separation valve 12 is connected to the sampler 11 via a front line 14 and to the first power means 13 via a rear line 15. The first power means 13 is used to drive the sampler 11 to collect a blood sample and draw the collected blood sample through the front line 14 into the blood separation valve 12. Wherein the blood separation valve 12 is used to separate a blood sample into a first portion of the blood sample and a second portion of the blood sample.
The blood sedimentation detection module 20 comprises a detection line 21 and an optical detection device 22. A test line 21 is connected to the blood separation valve 12 and is used to provide a test site for a first portion of the blood sample dispensed by the blood separation valve 12. The optical detection device 22 is disposed corresponding to the detection line 21, and is configured to irradiate a first portion of the blood sample in the detection line 21 with light and detect an absorption or scattering degree of the first portion of the blood sample in the detection line 21 with light, so as to obtain a erythrocyte sedimentation rate of the first portion of the blood sample. When the erythrocyte sedimentation rate detection module 20 detects the erythrocyte sedimentation rate, the first part of the blood sample does not displace in the detection pipeline 21, i.e. remains stationary.
The blood routine detection module 30 includes a blood routine detection cell 31 and a blood routine detection device (not shown). A blood routine testing reservoir 31 is connected to the blood separation valve 12 and is adapted to provide a testing site for a second portion of the blood sample dispensed by the blood separation valve 12, the blood routine testing device performing a blood routine test on the second portion of the blood sample in the blood routine testing reservoir 31.
In particular, the first power means 13 is adapted to provide a negative pressure to draw the blood sample within the sampler 11 into the blood separation valve 12. The first power means 13 may be a pump, syringe or other source of power-providing pressure, such as a source of positive and negative air pressure.
In this embodiment, the blood separation valve 12 has two different operating states. In a first operating state, as shown in fig. 2 and 3, after the blood sample is collected by the sampler 11, the first power device 13 draws the blood sample in the sampler 11 through the front pipe 14 and the rear pipe 15 into the blood separating valve 12, and the blood separating valve 12 separates the blood sample into a first partial blood sample and a second partial blood sample.
As shown in fig. 1, the blood separating valve 12 is switched to the second working state, the blood separating valve 12 is respectively connected with the detecting pipeline 21 of the blood sedimentation detecting module 20 and the blood sedimentation detecting tank 31 of the blood sedimentation detecting module 30, a first part of blood sample enters the blood sedimentation detecting pipeline 21 to detect the erythrocyte sedimentation rate, and a second part of blood sample enters the blood sedimentation detecting tank 31 to detect the blood sedimentation parameter.
The blood routine testing tank 31 described herein is connected to the blood dividing valve 12, and may refer to a connection made by providing a pipeline between the blood dividing valve 12 and the blood routine testing tank 31. It may also be that only a liquid path connection is performed between the blood routine detection cell 31 and the blood separation valve 12, so long as the second portion of the blood sample in the blood separation valve 12 can be distributed to the blood routine detection cell 31, and the specific embodiment is not specifically limited herein.
The blood routine detection device may be an optical detection device or an impedance detection device. When the blood sample is subjected to the blood routine detection by the blood routine detection module 30, the blood sample and the corresponding reagent may be added to the blood routine detection cell 31, and the blood sample in the blood routine detection cell 31 may be measured by the blood routine detection device to obtain at least one blood routine parameter, where the blood routine parameter may include at least one or a combination of WBC (White blood cell) five classification result, WBC count and morphological parameters, HGB (Hemoglobin) function measurement, RBC (Red blood cell) count and PLT (platelets) count and morphological parameters. In the actual blood routine detection process, blood routine detection items can be increased or decreased as required, and are not limited herein.
The working process of the sample analyzer provided by the embodiment of the application is as follows:
The first power means 13 drives the sampler 11 to collect a blood sample and draws the blood sample collected by the sampler 11 through the front line 14 into the blood dividing valve 12, the blood dividing valve 12 dividing the blood sample into a first part of the blood sample and a second part of the blood sample.
The first partial blood sample is dispensed to the detection line 21, and the optical detection device 22 irradiates the first partial blood sample in the detection line 21 with light and detects the degree of absorption or scattering of the light by the first partial blood sample, thereby detecting the erythrocyte sedimentation rate of the first partial blood sample.
The second portion of the blood sample is dispensed into the blood routine testing cell 31, and the blood routine testing device performs blood routine testing on the second portion of the blood sample in the blood routine testing cell 31.
The sample analyzer provided by the embodiment of the application comprises a blood separating valve 12, wherein the blood separating valve 12 is connected with a sampler 11 through a front pipeline 14 and is connected with a first power device 13 through a rear pipeline 15. The blood separating valve 12 is further connected to the detecting pipeline 21 and the blood conventional detecting tank 31, so that after the blood sample is collected by the sampler 11, the blood separating valve 12 can distribute the blood sample into a first part of blood sample and a second part of blood sample, and the first part of blood sample is distributed to the detecting pipeline 21 and the second part of blood sample is distributed to the blood conventional detecting tank 31, so that the blood sedimentation detecting module 20 and the blood conventional detecting module 30 can respectively and independently detect the blood cell sedimentation rate and the blood conventional detecting parameter in parallel, the detection of the next detecting module can be performed without waiting for the detection of the last detecting module, the total detecting time is short, and the detecting efficiency is high.
In the sample analyzer provided by the embodiment of the application, the blood sedimentation detection module 20 and the blood routine detection module 30 can be started to detect simultaneously, or the blood sedimentation detection module 20 and the blood routine detection module 30 can be started to detect sequentially, so long as the detection time of the blood sedimentation detection module 20 and the blood routine detection module 30 is overlapped, the parallel detection can be realized, and the total duration of the two detection projects can be shortened, and the method is not particularly limited.
Unlike the prior art in which the blood sedimentation detection module 20 and the blood routine detection module 30 are serially connected to sequentially perform corresponding erythrocyte sedimentation rate detection and blood routine detection, the sample analyzer provided by the embodiment of the application includes the blood sedimentation detection module 20 and the blood routine detection module 30 which can perform detection independently, and the detection of the next detection module can be performed without waiting for the detection of the last detection module, so that the time of detecting the erythrocyte sedimentation rate of the first portion of the blood sample by the blood sedimentation detection module 20 overlaps with the time of detecting the blood routine parameter of the second portion of the blood sample by the blood routine detection module 30, thereby enabling the time of parallel detection of the blood sedimentation detection module 20 and the blood routine detection module 30 to be shortened, so that the total duration of detecting two items is shortened, and the detection efficiency is further improved.
As shown in fig. 3, the sampler 11 according to the embodiment of the present application includes a sampling needle 111 and a driving device (not shown) for driving the sampling needle 111 to move to a position where a test tube storing a blood sample is located so as to collect the blood sample in the test tube. Specifically, the driving device may be a mechanical structure capable of driving the sampling needle 111 to move, and is not particularly limited herein.
As shown in fig. 3, a blood sample is generally stored in a test tube 100, and the top end of the test tube 100 is provided with a cap for sealing. The driving means drives the sampling needle 111 to move to the corresponding test tube 100, and the end of the sampling needle 111 with the needle tip pierces the cap of the test tube 100 and then extends into the test tube 100 to suck the blood sample in the test tube 100, thereby collecting the blood sample.
Specifically, the first power means 13 also provides a negative pressure to the sampling needle 111 to enable the sampling needle 111 to draw a blood sample from within the test tube 100.
In one embodiment of the present application, the blood separation valve 12 includes a liquid inlet 121, a first liquid separation port, a second liquid separation port, and a liquid outlet 122.
As shown in fig. 3, the liquid inlet 121 is connected with the sampler 11 through the front pipeline 14, and the first power device 13 drives the blood sample collected by the sampler 11 to flow into the blood separating valve 12 through the liquid inlet 121.
As shown in fig. 1, a first liquid separation port is connected to the detection line 21, and the first liquid separation port is used for allowing a first portion of the blood sample dispensed from the blood separation valve 12 to flow into the detection line 21. That is, the blood separation valve 12 distributes a first portion of the blood sample into the test line 21 via the first sample transfer passage. The second port is connected to the blood conventional measuring cell 31, and the second port is used for the second portion of the blood sample dispensed by the blood dividing valve 12 to flow into the blood conventional measuring cell 31. That is, the blood separation valve 12 distributes the second portion of the blood sample into the blood conventional detection cell 31 through the second sample transmission channel.
As shown in fig. 3, the liquid outlet 122 is connected to the first power device 13 through the rear pipeline 15, and the first power device 13 drives the blood sample in the blood separating valve 12 to flow from the liquid outlet 122 into the rear pipeline 15. For example, after the test is completed, the first power device 13 may drive the remaining blood sample within the blood separation valve from the fluid outlet 122 into the rear tubing 15 for further draining.
In one embodiment of the present application, as shown in fig. 1 and 2, the blood separation valve 12 includes an outer sheet 123, a middle sheet 124, and an inner sheet 125 coaxially attached in this order.
The outer plate 123 is provided with a liquid inlet channel 1231, a liquid outlet channel 1232, a first outer liquid separating channel 1233 and a second outer liquid separating channel 1234, one port of the liquid inlet channel 1231 is a liquid inlet 121, and one port of the liquid outlet channel 1232 is a liquid outlet 122.
The middle piece 124 is provided with a first middle liquid separating channel 1241 and a second middle liquid separating channel 1242.
The inner piece 125 is provided with a connecting channel 1251, a first inner liquid separating channel 1252 and a second inner liquid separating channel 1253, a port of the first inner liquid separating channel 1252 far from the first inner liquid separating channel 1241 is a first liquid separating port, and a port of the second inner liquid separating channel 1253 far from the second inner liquid separating channel 1242 is a second liquid separating port.
The middle piece 124 can move relative to the outer piece 123 and the inner piece 125, so that the relative position between the middle piece 124 and the outer piece 123 and the inner piece 125 is changed, and the working state of the blood dividing valve 12 is changed.
In one embodiment of the present application, outer plate 123 and inner plate 125 are stationary and middle plate 124 is rotatable coaxially with respect to outer plate 123 and inner plate 125.
Referring to the operation timing chart of the sample analyzer shown in fig. 4, in one embodiment of the present application, the operation of the sample analyzer is as follows:
The middle piece 124 is rotated so that the blood separating valve 12 is in the first operating state, namely: the liquid inlet passage 1231, the first intermediate liquid separation passage 1241, the connection passage 1251, the second intermediate liquid separation passage 1242, and the liquid outlet passage 1232 are sequentially communicated. The first power means 13 provides power (e.g., negative pressure) such that the blood sample in the sampling needle 111 sequentially passes through the front tube 14, the liquid inlet passage 1231, the first middle liquid dividing passage 1241, the second middle liquid dividing passage 1242, the connection passage 1251, the liquid outlet passage 1232, and the rear tube 15, i.e., such that the blood sample collected by the sampling needle 111 flows in the blood dividing valve 12 and fills the liquid inlet passage 1231, the first middle liquid dividing passage 1241, the second middle liquid dividing passage 1242, the connection passage 1251, and the liquid outlet passage 1232, thereby enabling the blood sample to be divided into the first portion blood sample and the second portion blood sample. In the embodiment shown in fig. 1 and 2, the blood sample in the first middle split channel 1241 is a first portion of the blood sample and the blood sample in the second middle split channel 1242 is a second portion of the blood sample.
The middle plate 124 is rotated again so that the blood separating valve 12 is in the second operating state, namely: the first middle liquid separation channel 1241 communicates with the first outer liquid separation channel 1233 and the first inner liquid separation channel 1252, respectively, to form a first sample transmission channel; and the second middle liquid separation channel 1242 communicates with the second outer liquid separation channel 1234 and the second inner liquid separation channel 1253, respectively, to form a second sample transmission channel, so that the first portion of the blood sample in the first middle liquid separation channel 1241 and the second portion of the blood sample in the second middle liquid separation channel 1242 can be simultaneously distributed into the detection tube 21 and the blood conventional detection cell 31.
In one embodiment, the first outer fluid separation channel 1233 is coupled to a power source (not shown) via tubing such that a first portion of the blood sample within the first inner fluid separation channel 1241 can be driven through the first inner fluid separation channel 1252 and into the test tubing 21. In another embodiment, a first portion of the blood sample in the first internal fluid passageway 1241 may be drawn through the first internal fluid passageway 1252 and into the test line 21 by a power source connected to the test line 21.
In one embodiment, the second external fluid separation channel 1234 is coupled to a power source (not shown) via tubing such that a second portion of the blood sample within the second internal fluid separation channel 1242 can be driven through the second internal fluid separation channel 1253 and into the blood conventional test cell 31. The power source connected to the second outer fluid separation channel 1234 includes a pump or syringe and a source of diluent, which pumps the diluent and then pushes the pumped diluent to the second outer fluid separation channel 1234, thereby pushing the second portion of the blood sample in the second middle fluid separation channel 1242 through the diluent into the blood conventional test cell 31.
It will be appreciated by those skilled in the art that although fig. 1 shows only one second sample transmission channel formed by the communication of the second outer fluid separation channel 1234, the second middle fluid separation channel 1242 and the second inner fluid separation channel 1253, embodiments of the application are not limited thereto. Blood routine testing devices may be used to test for multiple of reticulocytes, nucleated red blood cell assays, white blood cells, hemoglobin assays, and red blood cells. Accordingly, the blood separation valve 12 includes a plurality of sample transmission channels among the reticulocyte measurement channel, the nucleated red blood cell analysis channel, the white blood cell analysis channel, the hemoglobin analysis channel, and the red blood cell measurement channel, and the blood sample collected by the sampler 11 is quantitatively separated by the blood separation valve 12 and then distributed through the corresponding channels.
According to the sample analyzer provided by the embodiment of the application, the first liquid separating port of the blood separating valve 12 is connected with the detection pipeline 21, and the second liquid separating port is connected with the blood routine detection tank 31, so that the first part of blood sample and the second part of blood sample can be simultaneously distributed, further, the blood sedimentation detection module 20 and the blood routine detection module 30 can respectively and independently detect the blood cell sedimentation rate and the blood routine detection parameter in parallel, the detection of the next detection module can be performed without waiting for the detection of the last detection module, the total detection time is short, and the detection efficiency is high.
In the embodiment of the application, after the first part of blood sample and the second part of blood sample are simultaneously distributed, the erythrocyte sedimentation rate and the blood routine parameter can be simultaneously started and detected by the blood sedimentation detection module 20 and the blood routine detection module 30 without waiting time, so that the total detection time is short, and the detection efficiency is further improved.
The blood sedimentation detection module 20 and the blood routine detection module 30 may start detection at the same time, or may start detection sequentially, for example, the blood routine detection module 30 starts blood routine parameter detection first, and starts erythrocyte sedimentation rate detection after the blood sedimentation detection module 20, which is not limited herein.
In addition, after the detection ends, the blood sedimentation detection module 20 and the blood routine detection module 30 need to be cleaned to perform the next detection, so as to avoid the residual of the previous blood sample to be detected from affecting the detection result of the next blood sample to be detected. The sample distribution module 10 also requires cleaning to collect the next blood sample to be tested.
Fig. 5 is a schematic structural diagram of a sample analyzer according to an embodiment of the present application. As shown in fig. 5, the blood sedimentation detection module 20 further includes a blood sedimentation detection tank 23 independent of the blood sedimentation conventional detection tank 31, the detection line 21 being connected to the blood separation valve 12 through the blood sedimentation detection tank 23, the blood sedimentation detection tank 23 being for receiving a first portion of the blood sample dispensed by the blood separation valve 12.
The detection pipeline 21 provided by the embodiment of the application is connected with the blood sedimentation detection tank 23 and the blood separation valve 12 through the blood sedimentation detection tank 23, which can mean that the blood sedimentation detection tank 23 is connected with the blood separation valve 12 through the pipeline, and a first part of blood sample in the blood separation valve 12 is injected into the blood sedimentation detection tank 23 through the pipeline.
In one embodiment of the application, as shown in fig. 1 and 5, the blood sedimentation testing module 20 further comprises a second power device 24 connected to the testing line 21, the second power device 24 being configured to drive the first portion of the blood sample dispensed by the blood separation valve 12 to flow into the testing line 21 and stop and hold the first portion of the blood sample after flowing into the testing area within the testing line 21, so that the optical testing device 22 can test the erythrocyte sedimentation rate of the first portion of the blood sample. That is, the second power device 24 is used not only to drive the first portion of the blood sample into the test line 21, but also to ensure that the first portion of the blood sample in the test line 21 remains stationary while the erythrocyte sedimentation rate test is performed.
Specifically, the working process for detecting ESR provided by the embodiment of the application is as follows:
The second power device 24 drives the flow of the blood sample into the detection line 21, and after the blood sample flows into a specific position (detection area) of the detection line 21, the second power device 24 instantaneously interrupts the flow of the blood sample in the detection line 21, thereby causing the blood sample to suddenly decelerate (or stop flowing) at this time, and then aggregation and sedimentation of red blood cells occur. During the aggregation and precipitation of the erythrocytes, a change in the signal detected by the optical detection means 22 will be caused, so that information for determining the ESR is obtained.
Compared with the Wilcet method in the prior art, the sample analyzer provided by the embodiment of the application can limit the length of the detection pipeline 21, not only can reduce the volume of the whole instrument, but also can reduce the blood volume required by detection analysis and the consumption of cleaning liquid for cleaning the detection pipeline 21.
In the embodiment shown in fig. 1, the test line 21 is connected at one end directly to the blood separation valve 12 and at the other end to a second power device 24, the second power device 24 driving a first portion of the blood sample in the blood separation valve 12 into the test line 21.
Whereas in the embodiment shown in fig. 5 one end of the detection line 21 is connected to the blood sedimentation detection tank 23 indirectly, the other end of the detection line 21 is connected to a second power means 24, and the second power means 24 drives a first portion of the blood sample in the blood sedimentation detection tank 23 dispensed by the blood sedimentation detection tank 12 into the detection line 21.
After detecting the erythrocyte sedimentation rate, the second power device 24 may also drive the flow of the first portion of the blood sample in the detection line 21 to exit the detection line 21 in preparation for the next detection.
The second power device 24 may be a pump, syringe, or other source of pressure capable of providing power.
Fig. 6 is a schematic structural diagram of a sample analyzer according to an embodiment of the present application. As shown in fig. 6, the detection pipeline 21 provided in the embodiment of the present application is directly disposed in the rear pipeline 15, that is, a portion of the rear pipeline 15 is the detection pipeline 21. In one embodiment, the portion of the rear line 15 adjacent to the blood separation valve 12 is provided as the detection line 21. At this time, the first power device 13 draws the blood sample collected by the sampler 11 into the blood separation valve 12 until a part of the blood sample flows into the detection line 21 of the rear line 15, and the blood sample in the detection line 21 of the rear line 15 is the first part of the blood sample. While the blood separation valve 12 still distributes the second portion of the blood sample through its sample transfer passage to the blood conventional test cell 31, as described above.
That is, the detection pipeline 21 is directly coupled to the liquid outlet 122 of the blood separating valve 12, so that in the first working state of the blood separating valve 12, the first power device 13 drives the blood sample in the sampling needle 111 to flow into the rear pipeline 15, i.e. the detection pipeline 21, through the liquid inlet channel 1231, the first middle liquid separating channel 1241, the connecting channel 1251, the second middle liquid separating channel 1242 and the liquid outlet channel 1232 in sequence, and the part of the blood sample in the rear pipeline 15 is the first part of the blood sample. At this time, the first power device 13 may function as the above-described second power device 24, and therefore, it is not necessary to additionally provide the second power device 24 in this embodiment.
The first internal fluid channel 1252 or the second internal fluid channel 1253 of the blood separating valve 12 in the sample analyzer according to the embodiment of the application is connected to the blood conventional detecting cell 31. When the blood separation valve 12 is in the first operating state, the first middle liquid separation channel 1241 or the second middle liquid separation channel 1242 is filled with the blood sample. When the blood separation valve 12 is switched to the second operation state, the blood sample in the first middle liquid separation channel 1241 or the second middle liquid separation channel 1242 can flow into the blood routine detection cell 31 through the first middle liquid separation channel 1252 or the second middle liquid separation channel 1253 which are connected correspondingly, so as to detect blood routine parameters.
Referring to the operation timing chart of the sample analyzer shown in fig. 7, the operation procedure of the sample analyzer according to an embodiment of the present application is as follows:
Rotating the middle plate 124 places the blood separation valve 12 in a first operating state, namely: the liquid inlet passage 1231, the first intermediate liquid separation passage 1241, the connection passage 1251, the second intermediate liquid separation passage 1242, and the liquid outlet passage 1232 are sequentially communicated. The first power device 13 provides power (e.g., negative pressure) such that the blood sample in the sampling needle 111 sequentially passes through the front tube 14, the liquid inlet channel 1231, the first middle liquid dividing channel 1241, the second middle liquid dividing channel 1242, the connecting channel 1251, the liquid outlet channel 1232 until entering the rear tube 15, such that the blood sample fills at least the liquid inlet channel 1231, the first middle liquid dividing channel 1241, the second middle liquid dividing channel 1242, the connecting channel 1251, and the liquid outlet channel 1232. At this time, a part of the blood sample is also present in the rear line 15, and by disposing the detection line 21 of the blood sedimentation detection module 20 in the rear line 15, that is, disposing the optical detection device 22 of the blood sedimentation detection module 20 at the rear line 15, the blood sample present in the rear line 15 can be directly used as the first part of the blood sample for the erythrocyte sedimentation rate detection.
The middle plate 124 is then rotated to switch the blood separation valve 12 to a second operating state, namely: the first middle liquid separation channel 1241 communicates with the first outer liquid separation channel 1233 and the first inner liquid separation channel 1252, respectively, to form a first sample transmission channel; and the second middle liquid separation channel 1242 communicates with the second outer liquid separation channel 1234 and the second inner liquid separation channel 1253, respectively, to form a second sample transmission channel, so that the blood sample in the first middle liquid separation channel 1241 or the second middle liquid separation channel 1242 can flow into the blood routine detection cell 31.
The blood sedimentation detection module 20 and the blood routine detection module 30 may then be activated for detection simultaneously or sequentially.
That is, the blood sedimentation detection module 20 is directly structurally coupled to the rear line 15 of the blood separation valve 12. After the sample distribution module 10 has sucked the sample, the blood sedimentation detection module 20 can be started to detect the erythrocyte sedimentation rate, as shown in fig. 7. The blood may be first separated from the blood routine detection module 30 by the blood separation valve 12, and then the erythrocyte sedimentation rate detection and the blood routine detection may be started simultaneously or in a time-sharing manner. At this time, the pipeline of the sampling and dispensing module 10 can be cleaned after the blood sedimentation detection module 20 completes detection.
The sample analyzer provided by the embodiment of the application directly arranges the detection pipeline 21 in the rear pipeline 15, namely, multiplexes the rear pipeline 15 into the detection pipeline 21, so that not only can the blood sample existing in the rear pipeline 15 be fully utilized, but also the second power device 24 can be omitted, the structure is simple, and the cost is saved.
As shown in fig. 1, 5 and 6, in the embodiment of the present application, the optical detection device 22 includes an optical transmitter 221 and an optical receiver 222. The light emitter 221 and the light receiver 222 are located on both sides of the detection area of the detection line 21, respectively. The light emitter 221 is used to illuminate a first portion of the blood sample within the detection zone. The light receiver 222 is configured to detect an amount of change in the light emitted from the light emitter 221 after the light irradiates the first portion of the blood sample (e.g., to receive light transmitted by and/or scattered by the first portion of the blood sample), and to detect an absorption or scattering degree of the light by the first portion of the blood sample by detecting the amount of the received light.
Upon activation of the blood sedimentation detection module 20 for detection, the second motive device 24 drives the flow of the first portion of the blood sample into the detection line 21 and stops the movement of the first portion of the blood sample after it has flowed into the detection zone, and then holds the first portion of the blood sample stationary in the detection zone. The light emitter 221 irradiates the first portion of the blood sample in the detection area with light, and the light receiver 222 detects the degree of scattering or transmission of the light emitted by the light emitter 221 after the light irradiates the first portion of the blood sample in the detection area, that is, detects the erythrocyte sedimentation rate by detecting the amount of light received by the light receiver 222.
Since the scattering or transmission of the light irradiated to the blood sample is changed during the aggregation (rouleaux formation) of the red blood cells in the blood sample, the scattering or absorption degree of the first portion of the blood sample to the light can be detected by detecting the amount of the light transmitted or scattered after the irradiation of the blood sample, thereby measuring the sedimentation rate of the red blood cells.
The number of the light receivers 222 is one or more, which is not limited herein.
In one embodiment of the present application, the detection line 21 is made of a flexible tube, and the detection area of the detection line 21 is made of a light-transmitting material. Therefore, the detection pipe 21 may be flexibly arranged, for example, may be vertically, horizontally or obliquely arranged, or may be bent, which is not limited.
In one embodiment of the application, the test line 21 is formed as a capillary tube.
Because the blood sedimentation detection module 20 provided in the sample analyzer of the present embodiment detects the sedimentation rate of red blood cells by detecting the scattering or absorption degree of red blood cells in a blood sample to light during the aggregation process (that is, detecting the aggregation speed of red blood cells to realize ESR detection), compared with the detection mode (the weisse method) of waiting for natural sedimentation of red blood cells according to the action of gravity, the detection speed is faster, the detection of sedimentation rate of red blood cells can be completed in a short time (for example, 20 s), and the blood consumption is less, which is only about 100uL. Furthermore, for the weissell method, it is necessary to use a hard detection tube that is arranged straight and vertically or slightly inclined, thereby easily making the instrument excessively bulky. The setting angle of the detection pipeline 21 provided by the embodiment of the application is not limited, and the detection pipeline can be flexibly adjusted according to the setting of other structures in the sample analyzer, so that the whole volume of the sample analyzer can be reduced, and the occupied space of the sample analyzer is small.
Fig. 8 is a schematic structural diagram of a sample analyzer according to an embodiment of the present application. As shown in fig. 8, the sample analyzer provided in the embodiment of the present application further includes a fluid path support module 40 for providing fluid path support for the sampling distribution module 10, the blood sedimentation detection module 20, and the blood routine detection module 30. Specifically, the fluid circuit support module 40 performs fluid circuit support by supplying the liquid to the sampling distribution module 10, the blood sedimentation detection module 20, and the blood routine detection module 30. For example, the liquid path support module 40 may respectively provide the sampling and dispensing module 10, the blood sedimentation detection module 20, and the blood routine detection module 30 with cleaning liquid to clean the sampling needle 111, the detection pipeline 21, and the blood routine detection cell 31, respectively, so as to avoid polluting the blood sample to be detected and causing inaccurate detection results.
In an embodiment, reagent sample adding, reaction uniform mixing, measurement action, cleaning maintenance and the like of the blood sedimentation detection module and the blood routine detection module are completed by the aid of the liquid path support module.
As shown in fig. 8, the sample analyzer further includes a control module 50, where the control module 50 is respectively connected to the sampling and dispensing module 10, the blood sedimentation detection module 20, the blood routine detection module 30, and the fluid path support module 40, and the control module 50 is configured to respectively control actions of the sampling and dispensing module 10, the blood sedimentation detection module 20, the blood routine detection module 30, and the fluid path support module 40, so that the sampling and dispensing module 10, the blood sedimentation detection module 20, the blood routine detection module 30, and the fluid path support module 40 cooperate with each other to complete detection of erythrocyte sedimentation rate and blood routine parameters.
The embodiment of the application also provides a sample analysis method, as shown in fig. 9, comprising the following steps:
Sample distribution step S200: the first power device 13 drives the sampler 11 to collect a blood sample and draws the blood sample in the sampler 11 into the blood separation valve 12 through the front pipeline 14 connecting the sampler 11 with the blood separation valve 12 so as to divide the blood sample into a first part of blood sample and a second part of blood sample and distribute the first part of blood sample and the second part of blood sample to the blood sedimentation detection module 20 and the blood routine detection module 30, respectively;
Erythrocyte sedimentation rate detection step S210: the blood sedimentation detection module 20 irradiates light to the first part of blood sample and detects the absorption or scattering degree of the first part of blood sample to obtain the erythrocyte sedimentation rate of the first part of blood sample;
Blood routine detection step S230: the blood routine testing device 30 tests blood routine parameters of the second portion of the blood sample.
According to the sample analysis method provided by the embodiment of the application, after the blood sample is collected by the sampler 11 and conveyed to the blood separating valve 12, the blood separating valve 12 separates the blood sample into the first part of blood sample and the second part of blood sample, and then the first part of blood sample flows into the blood sedimentation detection module 20 and the second part of blood sample flows into the blood routine detection module 30 respectively, so that different parts of the same blood sample are distributed to the blood sedimentation detection module 20 and the blood routine detection module 30, and further, the blood sedimentation detection module 20 and the blood routine detection module 30 can be independently and parallelly detected respectively, the detection of the next detection module can be performed without waiting for the detection of the last detection module, the total detection time is short, and the detection efficiency is improved.
In the sample analysis method provided by the embodiment of the application, the time for detecting the erythrocyte sedimentation rate of the first part of blood sample by the blood sedimentation detection module 20 overlaps with the time for detecting the blood conventional parameter of the second part of blood sample by the blood conventional detection module 30, namely, the time overlap exists between the step 210 and the step 220, so that the blood sedimentation detection module 20 and the blood conventional detection module 30 can perform parallel detection in time, and the total duration for detecting the two items is shortened.
In the sample analysis method provided in the embodiment of the present application, the time for the blood sedimentation detection module 20 to start detecting the erythrocyte sedimentation rate of the first portion of the blood sample is the same as or different from the time for the blood routine detection module 30 to start detecting the blood routine parameter of the second portion of the blood sample, which is not specifically limited herein.
Optionally, the blood sedimentation detection module 20 is started to detect the erythrocyte sedimentation rate of the first portion of the blood sample after the blood routine detection module 30 is started to detect the blood routine parameters of the second portion of the blood sample. Because the ESR detection method used in the application is faster than the blood routine detection speed, the starting of the blood routine detection is beneficial to the quick total detection report.
In other embodiments, the blood sedimentation detection module 20 may be started to detect the erythrocyte sedimentation rate of the first portion of the blood sample, and then the blood routine detection module 30 may be started to detect the blood routine parameter of the second portion of the blood sample; or simultaneously activates the blood sedimentation detection module 20 and the blood routine detection module 30 for detection, without limitation.
In an embodiment of the present application, the sample distribution step S200 includes:
The blood separation valve 12 distributes a first portion of the blood sample to the detection line 21 of the blood sedimentation detection module 20 or to the blood sedimentation detection cell 23 connected to the detection line 21 through its first sample transmission channel;
at the same time, the blood separation valve 12 distributes a second portion of the blood sample to the blood routine detection cell 31 of the blood routine detection module 30 via its second sample transmission channel.
In an embodiment of the present application, the sample distribution step S200 includes:
The first power device 13 draws the blood sample in the sampler 11 into the blood separating valve 12 through the front pipeline 14 until a part of the blood sample flows into the rear pipeline 15 connecting the first power device 13 with the blood separating valve 12, and the blood sample in the rear pipeline 14 is a first part of the blood sample;
The blood separation valve 12 then distributes the second portion of the blood sample through its sample transmission channel to the blood routine detection cell 31 of the blood routine detection module 30.
The sample analysis method provided by the embodiment of the present application is applied to the sample analyzer, and further embodiments of the sample analysis method may refer to the description of the sample analyzer, which is not repeated herein.
It should be noted that in this document, relational terms such as "first" and "second" and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises an element.
The features mentioned above in the description, in the drawings and in the claims can be combined with one another at will as far as they are relevant within the present application. The features and advantages described for the sample analysis system according to the application apply in a corresponding manner to the sample analysis method according to the application and vice versa.
The foregoing is merely exemplary of embodiments of the present application to enable those skilled in the art to understand or practice the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (15)
1. A sample analyzer, comprising:
A sample distribution module comprising a sampler for collecting a blood sample, a blood separation valve connected to the sampler by a front line and to the first power device by a rear line, and a first power device for driving the sampler to collect a blood sample and drawing the collected blood sample into the blood separation valve by the front line, wherein the blood separation valve is for separating the blood sample into a first portion of blood sample and a second portion of blood sample;
A blood sedimentation detection module comprising a detection pipeline and an optical detection device, wherein the detection pipeline is connected with the blood separating valve and is used for providing a detection place for a first part of blood sample distributed by the blood separating valve, the optical detection device is used for carrying out light irradiation on the first part of blood sample in the detection pipeline and detecting the absorption or scattering degree of light by red blood cells in the first part of blood sample in the detection pipeline in the process of aggregation so as to obtain the aggregation speed of the red blood cells of the first part of blood sample, and the sedimentation rate of the red blood cells of the first part of blood sample is determined according to the aggregation speed;
a blood routine detection module, comprising a blood routine detection cell for providing a detection site for a second portion of the blood sample dispensed by the blood separation valve, and a blood routine detection device for performing a blood routine detection on the second portion of the blood sample in the blood routine detection cell;
the blood separating valve comprises an outer sheet, a middle sheet and an inner sheet which are sequentially and coaxially attached;
The outer piece is internally provided with a liquid inlet channel, a liquid outlet channel, a first outer liquid separation channel and a second outer liquid separation channel, one port of the liquid inlet channel is a liquid inlet, and one port of the liquid outlet channel is a liquid outlet;
a first middle liquid separation channel and a second middle liquid separation channel are formed in the middle piece;
The inner piece is internally provided with a connecting channel, a first inner liquid separating channel and a second inner liquid separating channel, wherein one port of the first inner liquid separating channel, which is far away from the first inner liquid separating channel, is a first liquid separating port, and one port of the second inner liquid separating channel, which is far away from the second inner liquid separating channel, is a second liquid separating port;
the middle piece can move relative to the outer piece and the inner piece, and the relative positions of the middle piece, the outer piece and the inner piece are changed.
2. The sample analyzer of claim 1, wherein the time at which the blood sedimentation detection module detects the erythrocyte sedimentation rate of the first portion of the blood sample overlaps with the time at which the blood routine detection module detects the blood routine parameter of the second portion of the blood sample.
3. The sample analyzer of claim 1, wherein the fluid inlet is connected to the sampler via the front pipeline, and the first power device drives the blood sample collected by the sampler to flow into the blood separation valve via the fluid inlet;
The first liquid separating port is connected with the detection pipeline and is used for enabling the first part of blood samples distributed by the blood separating valve to flow into the detection pipeline;
The second liquid separation port is connected with the blood routine detection tank and is used for enabling the second part of blood sample distributed by the blood separation valve to flow into the blood routine detection tank;
the liquid outlet is connected with the first power device through the rear pipeline, and the first power device drives the blood sample in the blood separating valve to flow from the liquid outlet to the rear pipeline.
4. The sample analyzer of claim 3, wherein the blood sedimentation detection module further comprises a blood sedimentation detection cell independent of the blood routine detection cell, the detection line being connected to the blood separation valve by the blood sedimentation detection cell, the blood sedimentation detection cell being configured to receive the first portion of the blood sample dispensed by the blood separation valve.
5. The sample analyzer of claim 1, wherein the blood sedimentation detection module further comprises a second motive device coupled to the detection line, the second motive device for driving the flow of the first portion of the blood sample dispensed by the blood separation valve into the detection line and stopping and holding the movement of the first portion of the blood sample after it has flowed to a detection zone within the detection line so that the optical detection device detects the erythrocyte sedimentation rate of the first portion of the blood sample.
6. A sample analyzer according to claim 1 or 2, wherein a portion of the rear line is the detection line, wherein the first motive means is for drawing the blood sample collected by the sampler into the blood separation valve until a portion of the blood sample flows into the detection line of the rear line.
7. The sample analyzer of claim 1, wherein the optical detection device comprises:
a light emitter located on one side of the detection zone of the detection circuit and adapted to illuminate the first portion of the blood sample within the detection zone,
And the light receiver is positioned at the other side of the detection area of the detection pipeline and is used for detecting the variation of the light emitted by the light emitter after the first part of blood sample is irradiated.
8. The sample analyzer of claim 1, wherein the detection line is horizontally disposed.
9. The sample analyzer of claim 1, further comprising a fluid path support module for providing fluid path support for the sample distribution module, the blood sedimentation detection module, and the blood routine detection module.
10. A sample analysis method employing the sample analyzer of claim 1, the sample analysis method comprising:
a sampling and distributing step: the first power device drives the sampler to collect a blood sample and draws the blood sample in the sampler into the blood separating valve through a front pipeline which connects the sampler with the blood separating valve, so that the blood sample is divided into a first part of blood sample and a second part of blood sample and is respectively distributed to a blood sedimentation detection module and a blood routine detection module;
And detecting the erythrocyte sedimentation rate: the blood sedimentation detection module irradiates the first part of blood sample, detects the absorption or scattering degree of red blood cells in the first part of blood sample to light in the process of aggregation, so as to obtain the aggregation speed of the red blood cells of the first part of blood sample, and determines the sedimentation rate of the red blood cells of the first part of blood sample according to the aggregation speed;
Blood routine detection: the blood routine detection device detects blood routine parameters of the second portion of the blood sample.
11. The sample analysis method of claim 10, wherein the time at which the blood sedimentation detection module detects the erythrocyte sedimentation rate of the first portion of the blood sample overlaps with the time at which the blood routine detection module detects the blood routine parameter of the second portion of the blood sample.
12. The sample analysis method according to claim 10 or 11, wherein the sample distribution step comprises:
The blood separation valve distributes the first part of blood sample to a detection pipeline of the blood sedimentation detection module or a blood sedimentation detection pond connected with the detection pipeline through a first sample transmission channel of the blood separation valve;
At the same time, the blood separation valve distributes the second portion of the blood sample to a blood routine detection cell of the blood routine detection module through a second sample transmission channel thereof.
13. The sample analysis method according to claim 10 or 11, wherein the sample distribution step comprises:
The first power device draws the blood sample in the sampler into a blood separating valve through a front pipeline until a part of the blood sample flows into a rear pipeline connecting the first power device and the blood separating valve, wherein the blood sample in the rear pipeline is the first part of blood sample;
The blood separation valve then distributes the second portion of the blood sample to a blood routine testing reservoir of the blood routine testing module via its sample transfer channel.
14. The sample analysis method of claim 10, wherein the time at which the blood sedimentation detection module initiates detection of the erythrocyte sedimentation rate of the first portion of the blood sample is the same as or different from the time at which the blood routine detection module initiates detection of the blood routine parameter of the second portion of the blood sample.
15. The method of claim 10, wherein the blood sedimentation detection module is activated to detect the first portion of the blood sample after the second portion of the blood sample is subjected to the blood routine parameter detection.
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| CN111886489A (en) | 2020-11-03 |
| WO2021097609A1 (en) | 2021-05-27 |
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