CN114062209A - Blood analyzer - Google Patents
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- CN114062209A CN114062209A CN202110878480.1A CN202110878480A CN114062209A CN 114062209 A CN114062209 A CN 114062209A CN 202110878480 A CN202110878480 A CN 202110878480A CN 114062209 A CN114062209 A CN 114062209A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 275
- 239000008280 blood Substances 0.000 title claims abstract description 275
- 238000001514 detection method Methods 0.000 claims abstract description 254
- 238000005070 sampling Methods 0.000 claims abstract description 181
- 238000004062 sedimentation Methods 0.000 claims abstract description 61
- 238000002955 isolation Methods 0.000 claims abstract description 33
- 239000003085 diluting agent Substances 0.000 claims abstract description 31
- 238000005259 measurement Methods 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims description 23
- 230000003287 optical effect Effects 0.000 claims description 22
- 210000003743 erythrocyte Anatomy 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 12
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 description 12
- 239000012530 fluid Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 230000006870 function Effects 0.000 description 6
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000004590 computer program Methods 0.000 description 3
- 230000001678 irradiating effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010261 blood fractionation Methods 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/385—Diluting, dispersing or mixing samples diluting by adsorbing a fraction of the sample
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Abstract
The present invention provides a blood analyzer, comprising: the sampling distribution module comprises a sampling device and a sample distribution device, wherein the sampling device is used for collecting blood samples and comprises a sampling needle and a first power device, the first power device is used for driving the sampling needle to collect the blood samples, and the sample distribution device is used for distributing the collected blood samples to different detection modules. Set up the isolation gas post between sampling needle and diluent and make the blood sample as far as possible diluted, thereby it makes the connecting line between the two shorter in order to reduce the blood sample loss to make the blood sedimentation detection module to set up for the setting of stewing of sampling needle to the precision of measurement has been improved.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a blood analyzer.
Background
In blood in vivo, erythrocytes are in a dispersed suspension because of the flow of blood and the mutual repulsion of negative charges on the surface of erythrocytes. When the isolated blood is in a standing state, the red blood cells sink due to the action of gravity. When in pathological state, the kind and the content of albumen in the blood plasma can change, will change the balance of electric charge in the blood, make the red blood cell surface negative charge reduce, and then make the red blood cell form rouleaux and accelerate the subsidence. Thus, the assessment of the condition can be aided by measuring the rate of Erythrocyte sedimentation within 1 hour, i.e. the Erythrocyte Sedimentation Rate (ESR).
In the application scenario of clinical examination, the blood routine is also an indispensable examination index. Therefore, there is a need for an integrated machine that can detect both ESR and blood routine, thereby meeting the clinical needs of blood testing programs. The all-in-one has the sampling module to divide blood respectively to blood conventional detection module and erythrocyte sedimentation rate detection module (ESR detection module), and the structural design of ESR detection module and sampling module is reasonable inadequately in present all-in-one to make the blood sample of distributing to the ESR module dilute by the diluent or lose greatly in connecting line, lead to measurement accuracy not enough.
Disclosure of Invention
In view of this, the present invention provides a blood analyzer to solve the problem of insufficient blood sedimentation detection and measurement accuracy. The technical scheme is as follows:
a blood analyzer, comprising: the sampling distribution module comprises a sampling device and a sample distribution device, the sampling device comprises a sampling needle and a first power device, the first power device is used for driving the sampling needle to collect blood samples, and the sample distribution device is used for distributing the collected blood samples to different detection modules; the erythrocyte sedimentation rate detection module comprises a detection pipeline and an optical detection device, the detection pipeline provides a detection place for the blood sample, and the optical detection device is used for irradiating the blood sample in the detection pipeline with light and detecting the absorption or scattering degree of the blood sample in the detection pipeline to the light so as to obtain the erythrocyte sedimentation rate of the blood sample; the blood routine detection module comprises a blood routine detection pool and a blood routine detection device, the blood routine detection pool provides a detection place for a blood sample, and the blood routine detection device is used for performing blood routine detection on the blood sample in the blood routine detection pool; the liquid path support module is used for providing liquid path support for the sampling distribution module, the blood sedimentation detection module and the blood routine detection module; the sampling device also comprises a sample sucking pipeline which is connected with the sampling needle and the first power device; the liquid path supporting module is connected to the sample sucking pipeline, at least part of the sample sucking pipeline is filled with diluent, and after the sampling needle collects a blood sample, an isolation gas column is arranged between the blood sample and the diluent.
Optionally, the volume of the isolated gas column is equal to or greater than 5 μ Ι _ and equal to or less than 20 μ Ι _.
Optionally, the volume of the isolated gas column is 10 μ L or more and 15 μ L or less.
Optionally, the detection pipeline is a part of the sample suction pipeline, and the optical detection device is arranged on two sides of the sample suction pipeline; the sample separation device is connected to the sample suction pipeline, and after sampling of the sampling needle is completed, the sample separation device sucks at least part of blood samples close to the isolation gas column to the detection pipeline.
Optionally, the volume distance from the detection pipeline to the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 μ L, and the preset volume distance is the sum of a preset maximum sample suction amount and the volume of the isolation gas column.
Optionally, the blood sedimentation detection module further comprises a heater, the heater is used for heating the blood sample in the detection pipeline, the distance from the heater to the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 μ L, and the preset volume distance is the sum of a preset maximum sample suction amount and the volume of the isolation gas column.
Optionally, the blood analyzer further comprises a controller, and the controller controls the sample distribution device to distribute the first part of the blood sample close to the needle point of the sampling needle to the blood routine detection pool and then distribute the second part of the blood sample close to the isolated gas column to the blood sedimentation measurement module.
Optionally, the detection pipeline is connected to the sample suction pipeline; the sample separation device is connected to the detection pipeline, and after sampling of the sampling needle is completed, the sample separation device absorbs at least part of blood samples close to the isolation gas column to the detection pipeline.
Optionally, the volume distance from the detection pipeline to the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 μ L, and the preset volume distance is the sum of a preset maximum sample suction amount and the volume of the isolation gas column.
Optionally, the blood routine detection pool is connected to the sample suction pipeline, the sample separation device is connected to the blood routine detection pool, and after sampling by the sampling needle is completed, the sample separation device sucks at least part of the blood sample close to the isolated gas column to the blood routine detection pool; the volume distance between the blood routine detection pool and the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 mu L, and the preset volume distance is the sum of the preset maximum sample suction amount and the volume of the isolation gas column.
A blood analyzer, comprising: the sampling distribution module comprises a sampling device for collecting blood samples, the sampling device comprises a sampling needle and a first power device, the first power device is used for driving the sampling needle to collect the blood samples, and the sample distribution device is used for distributing the collected blood samples to different detection modules; the erythrocyte sedimentation rate detection module comprises a detection pipeline and an optical detection device, the detection pipeline provides a detection place for the blood sample, and the optical detection device is used for irradiating the blood sample in the detection pipeline with light and detecting the absorption or scattering degree of the blood sample in the detection pipeline to the light so as to obtain the erythrocyte sedimentation rate of the blood sample; the blood routine detection module comprises a blood routine detection pool and a blood routine detection device, the blood routine detection pool provides a detection place for a blood sample, and the blood routine detection device is used for performing blood routine detection on the blood sample in the blood routine detection pool; the detection line is connected to the sampling needle, and the detection line is arranged stationary relative to the sampling needle in at least one direction of movement.
Optionally, the detection line is disposed on the sampling distribution module.
Optionally, the sampling dispensing module comprises a motion assembly to which the sampling needle is mounted and drive means for driving the motion assembly and the sampling needle in a predetermined direction; the detection pipeline is arranged on the motion assembly.
Optionally, the movement assembly includes a transverse movement assembly and a vertical movement assembly which are driven by the driving device to perform transverse movement and vertical movement, respectively, and the detection pipeline is disposed on the transverse movement assembly or the vertical movement assembly.
Optionally, the sedimentation detection module is disposed on the sampling distribution module.
Compared with the prior art, the invention has the following beneficial effects:
according to the blood analyzer provided by the invention, the isolation air column is arranged between the sampling needle and the diluent, so that the blood sample is diluted as little as possible, or the detection pipeline of the blood sedimentation detection module is arranged in a standing way relative to the sampling needle, so that the connection pipeline between the sampling needle and the detection pipeline is shorter, the blood sample loss is reduced, and the measurement precision is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 shows a schematic view of a blood analyzer of one embodiment;
FIG. 2 is a schematic view showing a partial structure of a blood analyzer according to an embodiment;
FIG. 3 shows a schematic view of a blood analyzer of an embodiment in which the instrument is in the process of aspirating a sample;
FIG. 4 shows a schematic view of a blood analyzer of an embodiment in which the instrument is in a routine blood fractionation process;
FIG. 5 shows a schematic view of a blood analyzer of an embodiment in which the instrument is in the process of blood sedimentation detection;
FIG. 6 is a schematic view showing a partial structure of a blood analyzer according to an embodiment;
fig. 7 is a schematic diagram showing a partial structure of a blood analyzer according to an embodiment.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
As shown in fig. 1-3, one embodiment of the present application provides a blood analyzer 100 including a sampling distribution module 10, a sedimentation detection module 20, and a blood-routine detection module 30, a fluid circuit support module 40, and a controller 50. The blood analyzer 100 may also include a protein detection module or other detection module for detecting a particular protein.
The sample dispensing module 10 is used to collect blood samples from the sample containers 200 and dispense the blood samples to a plurality of test modules. Specifically, the sampling distribution module 10 comprises a sampling device and a sample separating device for collecting blood samples, the sampling device comprises a sampling needle 11 and a first power device 12, and the first power device 12 is used for driving the sampling needle 11 to collect blood samples; the sample distribution device is used for distributing the collected blood samples to different detection modules. The sample distribution device can distribute the collected blood sample to different detection modules in various ways according to the structural arrangement of the detection modules, for example, the blood sample is distributed to different detection modules by a power source, or the blood sample is sucked from the sampling needle 11 to the detection cell or the detection pipeline 21 of the detection module. In particular, the first motive device 12 is used to provide a negative pressure to draw the blood sample of the sample container 200 into the sampling needle 11. The first motive device 12 may be a pump, syringe, or other source of pressure capable of providing motive power, such as a source of positive and negative air pressure.
It should be noted that the sampling device and the sample dividing device in the sampling and dispensing module 10 may include the same structure or different structures, for example, the sampling device includes a sampling needle 11 and a first power device 12, when a blood sample is collected, the first power device 12 provides negative pressure to draw the blood sample in the sample container 200 into the sampling needle 11; the sample separation device also comprises a sampling needle 11 and a first power device 12, when the blood sample is distributed, the first power device 12 provides negative pressure to suck the blood sample into the blood sedimentation detection module 20; the sample separation device may also include a powered device that provides positive or negative pressure to dispense the blood sample into the blood routine detection module 30.
The blood sedimentation detection module 20 comprises a detection line 21 and an optical detection device 22. The test line 21 is used to provide a test site for the blood sample. The optical detection device 22 is disposed corresponding to the detection pipeline 21, and is used for irradiating light to the blood sample in the detection pipeline 21 and detecting the degree of absorption or scattering of the light by the blood sample in the detection pipeline 21 so as to obtain the erythrocyte sedimentation rate of the blood sample. The optical detection device 22 comprises a light source 221 and an optical-to-electrical converter 222, and the light source 221 and the optical-to-electrical converter 222 are used for measuring the light absorption or light scattering of the blood sample in the detection pipeline 21. The blood sedimentation detection module 20 further comprises a heater 23 and a temperature sensor 24, and the temperature control of the detection pipeline 21 is realized by the heater 23 and the temperature sensor 24. The blood sedimentation detection module 20 may further include a line fixing block for fixing the detection line 21. In addition, the detection line 21 and the optical detection device 22 may also be separately provided, that is, the detection line may not be fixed at the optical detection device 22, and the detection line 21 moves along with the sampling needle in at least one movement direction, that is, the detection line 21 and the sampling needle 11 are stationary in at least one movement direction. In one mode, when the sampling needle 11 moves the sample container 200 for blood sample collection, the detection line 21 communicates with the sampling needle 11 and moves with the sampling needle 11; then the sampling needle distributes the blood sample, the first power device 12 provides negative pressure, the blood sample is sucked to the detection pipeline 21 (because the sampling needle 11 is connected to the pipeline, in fact, when the sampling needle moves, the pipeline also moves in the optical detection device 22, therefore, after the sampling needle finishes sampling, the blood sample is transported to the section of pipeline at the optical detection device 22, which is the detection pipeline) for detection. In another mode, a certain section of the pipeline connected to the sampling needle 11 may be set as a detection pipeline, for example, a section of the pipeline close to the sampling needle is set as a detection pipeline, so that after the sampling needle 11 finishes collecting a blood sample, the first power device 12 provides negative pressure to suck the blood sample into the detection pipeline, and then the sampling needle 11 moves to bring the detection pipeline to the optical detection device 22 for detection.
The blood routine detecting module 30 includes a blood routine detecting cell 31 and a blood routine detecting device (not shown). The blood routine detecting pool 31 is used for providing a detecting place for the blood sample, and the blood routine detecting device performs blood routine detection on the blood sample in the blood routine detecting pool 31.
The fluid path support module 40 is used for providing fluid path support for the sampling distribution module 10, the blood sedimentation detection module 20 and the blood routine detection module 30; fluid circuit support may include functional support for fluid actuation, reagent filling, fluid circuit cleaning, waste fluid removal, and the like. For example, the fluid path support module 40 may provide cleaning solutions to the sampling distribution module 10, the blood sedimentation detection module 20, and the blood routine detection module 30, respectively, so as to clean the sampling needle 11, the detection line 21, and the blood routine detection pool 31, respectively, and avoid polluting the blood sample to be detected and causing inaccurate detection results. In addition, the liquid path support module 40 can be connected with the sampling needle 11 through a pipeline, and the liquid path support module 40 keeps the pipeline filled with diluent, so that the processes of sampling, sample separation and the like are faster and more reliable.
The controller 50 is communicatively connected to the sampling distribution module 10, the blood sedimentation detection module 20, the blood routine detection module 30, and the fluid circuit support module 40 to process information and control the operation of the respective modules.
The sampling device also comprises a sample suction pipeline 13 which is connected with the sampling needle 11 and the first power device 12; the liquid path support module 40 is connected to the sample suction pipeline 13, at least a part of the sample suction pipeline 13 is filled with diluent, and after the sampling needle 11 collects a blood sample, an isolation gas column 60 is arranged between the blood sample and the diluent. Specifically, after the sampling needle 11 collects a blood sample, the blood sample is filled in the sampling needle 11, or in the sampling needle 11 and the sample suction line 13, and a section of isolating gas column 60 is filled between the blood sample and the diluent to isolate the blood sample from the diluent, so that the blood sample is not diluted. As an alternative embodiment, before the sample is sucked, the liquid path support module 40 fills a part of the sample sucking pipeline 13 with the diluent, and fills a part of the sample sucking pipeline 13 close to the sampling needle 11 and the sampling needle 11 with the gas, and after the sample is sucked, an isolation gas column 60 is formed between the blood sample and the diluent; as another alternative, before the sample is sucked, the liquid path support module 40 fills the sample sucking line 13 with diluent and the sampling needle 11 with gas, and after the sample is sucked, an isolation gas column 60 is formed between the blood sample and the diluent; as another alternative, before the sample is drawn, the liquid path support module 40 fills the sample drawing line 13 and the sampling needle 11 with the diluent, the sampling needle 11 draws a predetermined volume or a certain amount of gas before drawing the blood sample, and after the sample is drawn, an isolation gas column 60 is formed between the blood sample and the diluent. The gas may be air or other gas that is poorly soluble in the diluent and blood sample.
Preferably, the volume of the isolating gas column 60 is greater than or equal to 5 μ L and less than or equal to 20 μ L, and the isolating gas column 60 in this range can ensure the effect of isolating the blood sample and the diluent, and can also keep the sample suction pipeline 13 to have a proper length, so as to ensure the rapid sample suction and separation. Further, the volume of the isolation gas column 60 is 10 μ L or more and 15 μ L or less, and the effect of isolating the diluent and the blood sample by the isolation gas column 60 is more excellent in this range.
In this embodiment, as shown in fig. 2, the detection pipeline 21 is a part of the sample suction pipeline 13, and the optical detection devices 22 are disposed at two sides of the sample suction pipeline 13; the sample separation device is connected to the sample suction pipeline 13, and after the sampling needle 11 finishes sampling, the sample separation device sucks at least part of the blood sample close to the isolation gas column 60 to the detection pipeline 21. That is, the blood sedimentation detecting module 20 is disposed on the sample suction line 13 near the sampling needle 11, and a part of the sample suction line 13 is used as the detecting line 21, so that the detecting line 21 does not need to be separately disposed, and the structure is simplified. Based on this structure, since the blood sample distributed to the blood sedimentation module 20 needs to flow through a section of the sample suction line 13 to reach the detection line 21, and the sample suction line 13 is filled with the diluent, it is more necessary to provide the isolation gas column 60 between the blood sample and the diluent to reduce the dilution degree of the blood sample flowing through the sample suction line 13. Specifically, during the process that the sampling needle 11 sucks the sample and the sample dividing device distributes the blood sample to the blood routine detecting pool 31 and then sucks the blood sample to the detecting pipeline 21, the diluent and the isolating gas column 60 move along with the sample and the position change of the isolating gas column 60 in the sample sucking pipeline 13 can be observed by comparing fig. 3 to fig. 5. The volume of the detection line 21 is 10 μ L or more and 50 μ L or less. Preferably, the volume of the detection line 21 is 40 μ L so that the sample suction line 13 has a suitable length.
The volume distance between the detection pipeline 21 and the needle point of the sampling needle 11 is greater than the preset volume distance and less than or equal to 200 muL, further, the volume distance between the blood sedimentation detection module 20 and the needle point of the sampling needle 11 is greater than the preset volume distance and less than or equal to 200 muL, and the preset volume distance is the sum of the preset maximum sample suction amount and the volume of the isolation gas column 60. The maximum sample suction amount is the maximum volume of the blood sample sucked by the sampling needle 11 set by the blood analyzer 100 by default, and the volume of the blood sample actually collected each time is less than or equal to the maximum sample suction amount. The design can not only ensure that the distance between the detection pipeline 21 and the sampling needle 11 is close enough to reduce the dilution of the blood sample by the residual diluent in the pipeline and the blood residue on the pipe wall, but also ensure that the detection module and the isolation gas column 60 are not overlapped so as to avoid the influence of the processes of heating the blood sedimentation detection module 20 and the like on the isolation gas column 60 in the sample sucking and separating processes. It should be noted that the volume distance from the detection pipeline 21 to the needle point of the sampling needle 11 is the volume distance from the end of the detection pipeline 21 close to the sampling needle 11 to the needle point of the sampling needle 11; the volume distance from the blood sedimentation detection module 20 to the needle point of the sampling needle 11 is the volume distance from one end of the blood sedimentation detection module 20 close to the sampling needle 11 to the needle point of the sampling needle 11.
Specifically, the blood sedimentation detection module 20 further comprises a heater 23, the heater 23 is used for heating the blood sample in the detection line 21, and the distance from the heater 23 to the needle point of the sampling needle 11 is greater than the preset volume distance and less than or equal to 200 μ L. This design can guarantee heater 23 enough be close enough apart from sampling needle 11 thereby alleviate the sample by remaining diluent dilution in the pipeline and blood remains in the pipe wall, avoid heater 23's heat again to make isolation gas column 60 be heated the inflation to influence and inhale appearance precision or follow-up branch appearance precision.
For the distribution sequence of the blood samples, for samples that are only tested for one of the routine blood tests or the sedimentation tests, the controller 50 controls the sample distribution device to distribute at least part of the blood samples to the respective test cells or test lines 21; for samples needing to be subjected to two tests of blood routine and blood sedimentation detection, the controller 50 controls the sample separation device to firstly distribute a first part of blood sample close to the needle point of the sampling needle 11 to the blood routine detection pool 31 and then distribute a second part of blood sample close to the isolation gas column 60 to the blood sedimentation measurement module, so that only part of the blood sample subjected to the blood sedimentation detection flows through the sample suction pipeline 13, and the part of the blood sample subjected to the blood routine detection does not flow through the sample suction pipeline 13, and the measurement efficiency is improved. The controller 50 also controls the blood routine detecting module 30 to start measurement after the first part of blood sample is distributed, and controls the blood sedimentation detecting module 20 to start measurement after the second part of blood sample is distributed, so that the measurement efficiency is improved.
In another embodiment of the present invention, as shown in fig. 6, the detection line 21 is connected to the sample suction line 13. That is, the detection line 21 is no longer part of the sample suction line 13, but is connected to the sample suction line 13, and the blood sample flows through the sample suction line 13 to the detection line 21. After the sampling needle 11 finishes sampling, the second power device 25 in the sample separation device sucks at least part of the blood sample close to the isolated gas column 60 to the detection pipeline 21. At this time, it is preferable that the volume distance from the blood sedimentation detection module 20 to the needle tip of the sampling needle 11 is greater than a preset volume distance, which is the sum of the preset maximum sample suction amount and the volume of the isolation gas column 60, and is less than or equal to 200 μ L.
In another embodiment of the present invention, as shown in fig. 7, the erythrocyte sedimentation rate detection module 20 is connected to the sampling needle 11, and specifically, the detection line 21 is connected to the sampling needle 11, that is, one outlet of the sampling needle 11 is connected to the sample suction line 13, and the other outlet is connected to the erythrocyte sedimentation rate detection module 20. At this time, the volume distance between the blood sedimentation detection module 20 and the needle point of the sampling needle 11 is greater than the volume distance H between the outlet of the blood sedimentation detection module 20 and the needle point of the sampling needle 11 and is less than or equal to 200 muL. Since the detection pipeline 21 is closer to the sampling needle 11, the detection pipeline 21 may be not filled with the diluent or filled with the diluent but not provided with the isolation gas column. Of course, the detection pipeline 21 may also be filled with a diluent and provided with the isolation gas column 70, at this time, the volume distance from the erythrocyte sedimentation detection module 20 to the needle point of the sampling needle 11 is greater than the preset volume distance and less than or equal to 200 μ L, and the preset volume distance is the sum of the volume distance H from the outlet of the erythrocyte sedimentation detection module 20 to the needle point of the sampling needle 11 and the volume of the isolation gas column 70. Specifically, before the sampling needle 11 sucks the blood sample, the second power device 25 in the sample separation device sucks a certain amount of gas so that the isolation gas column 70 is formed before the blood sample and the diluent after the blood sample is sucked by the sampling needle 11.
In another embodiment of the invention, the blood routine detecting pool is connected to the sample sucking pipeline, the sample separating device is connected to the blood routine detecting pool, and after sampling by the sampling needle is completed, at least part of the blood sample close to the isolating gas column is sucked to the blood routine detecting pool by the sample separating device. Preferably, the volume distance between the blood routine detection pool and the needle point of the sampling needle is greater than the preset volume distance and less than or equal to 200 muL; furthermore, the volume distance between the blood routine detection module and the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 muL, and the preset volume distance is the sum of the preset maximum sample suction amount and the volume of the isolation gas column.
In another embodiment of the invention, as shown in fig. 3, the detection line 21 is connected to the sampling needle 11, the detection line 21 being arranged stationary with respect to the sampling needle 11 at least in one direction of movement. The detection line 21 is connected to the sampling needle 11, and the detection line 21 is directly connected to the sampling needle 11, and the detection line 21 is indirectly connected to the sampling needle 11 through other structures such as the sample suction line 13. In summary, the detection line 21 is stationary relative to the sampling needle 11 in at least one direction of movement, which is the direction of movement of the sampling needle 11. In this case, when the sampling needle 11 moves, the distance between the sampling needle 11 and the detection pipeline 21 in at least one direction is not changed, so that a short connecting pipeline can be arranged between the sampling needle 11 and the detection pipeline 21, and the problem that the connecting pipeline needs to be correspondingly lengthened when the sampling needle 11 moves relative to the detection pipeline 21 in the processes of sample suction, sample separation and the like does not need to be considered, so that the loss of blood in the connecting pipeline, the difficulty in cleaning and the like are reduced. When the connecting line is filled with the diluent, since the connecting line is set short, the degree of dilution of the blood sample flowing from the sampling needle 11 to the detection line 21 can be also reduced.
As an alternative embodiment, the detection line 21 is provided on the sample distribution module 10. As shown in fig. 3-5, the blood sedimentation detection module 20 is disposed on the sampling distribution module 10. The sampling distribution module 10 comprises a movement assembly to which the sampling needle 11 is mounted and drive means for driving the movement assembly and the sampling needle 11 in a predetermined direction; specifically, the sedimentation detection module 20 is disposed on the motion assembly. In this embodiment, the driving device may be a motor, and the motion assembly includes a transverse motion assembly 141 and a vertical motion assembly 142 that are driven by the driving device to perform transverse motion and longitudinal motion, respectively. The sample distribution module 10 also includes a cross rail 143 and a vertical rail 144. Lateral motion assembly 141 is mounted to lateral rails 143, drive means drive lateral motion assembly 141 along lateral rails 143, vertical rails 144 are mounted to lateral motion assembly 141, vertical motion assembly 142 is mounted to vertical rails 144, and drive means drive vertical motion assembly 142 along vertical rails 144. The sampling needle 11 is mounted to the vertical motion assembly 142. The detection pipeline 21 can be arranged on the transverse motion assembly 141 or the vertical motion assembly 142. In fig. 3-5, the detection line 21 is disposed in the lateral motion assembly 141. When the detection line 21 is disposed on the lateral movement assembly 141, the distance of the detection line 21 in the lateral direction with respect to the sampling needle 11 is kept constant; when the detection pipeline 21 is arranged on the vertical movement component 142, the distance between the detection pipeline 21 and the sampling needle 11 is always kept unchanged.
It will be appreciated that with respect to the structural arrangement of the motion assembly and the rails, it is also possible that the lateral rails 143 are mounted to the vertical motion assembly 142, in which case the sampling needle 11 is mounted to the lateral motion assembly 141. The detection pipeline 21 can be arranged on the transverse motion assembly 141 or the vertical motion assembly 142. When the detection pipeline 21 is arranged on the transverse movement assembly 141, the distance between the detection pipeline 21 and the sampling needle 11 is always kept constant, and when the detection pipeline 21 is arranged on the vertical movement assembly 142, the distance between the detection pipeline 21 and the sampling needle 11 in the vertical direction is kept constant.
It will be appreciated that the structure and movement of the moving assembly carrying the sampling needle 11 is not limited to the embodiments described above, for example, the driving device may drive the moving assembly to rotate, and likewise, the detection line 21 is stationary relative to the sampling needle 11 because the detection line 21 is disposed on the moving assembly.
Further, the blood sedimentation detection module 20 is arranged stationary relative to the sampling needle 11 in at least one direction of movement, and specifically, the blood sedimentation detection module 20 is arranged on the sampling distribution module 10, or on the motion assembly, or on the above-mentioned lateral motion assembly 141 or vertical motion assembly 142. The sedimentation detection module 20 comprises at least a detection line 21 and an optical detection device 22. The weight of the blood sedimentation detection module 20 is more than or equal to 20g and less than or equal to 50 g; the volume of the detection line 21 is 10 μ L or more and 50 μ L or less. Preferably, the volume of the detection line 21 is 40 μ L.
Those skilled in the art will appreciate that all or part of the functions of the various methods in the above embodiments may be implemented by hardware, or may be implemented by computer programs. When all or part of the functions of the above embodiments are implemented by a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: a read only memory, a random access memory, a magnetic disk, an optical disk, a hard disk, etc., and the program is executed by a computer to realize the above functions. For example, the program may be stored in a memory of the device, and when the program in the memory is executed by the processor, all or part of the functions described above may be implemented. In addition, when all or part of the functions in the above embodiments are implemented by a computer program, the program may be stored in a storage medium such as a server, another computer, a magnetic disk, an optical disk, a flash disk, or a removable hard disk, and may be downloaded or copied to a memory of a local device, or may be version-updated in a system of the local device, and when the program in the memory is executed by a processor, all or part of the functions in the above embodiments may be implemented.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. Variations of the above-described embodiments may be made by those skilled in the art, consistent with the principles of the invention.
Claims (15)
1. A blood analyzer, comprising:
the sampling distribution module comprises a sampling device and a sample distribution device, the sampling device is used for collecting blood samples and comprises a sampling needle and a first power device, the first power device is used for driving the sampling needle to collect the blood samples, and the sample distribution device is used for distributing the collected blood samples to different detection modules;
the erythrocyte sedimentation rate detection device comprises a blood sedimentation detection module and a control module, wherein the blood sedimentation detection module comprises a detection pipeline and an optical detection device, the detection pipeline provides a detection place for a blood sample, and the optical detection device is used for carrying out light irradiation on the blood sample in the detection pipeline and detecting the absorption or scattering degree of the blood sample in the detection pipeline to the light so as to obtain the erythrocyte sedimentation rate of the blood sample;
the blood routine detection module comprises a blood routine detection pool and a blood routine detection device, the blood routine detection pool provides a detection place for a blood sample, and the blood routine detection device is used for performing blood routine detection on the blood sample in the blood routine detection pool;
the liquid path support module is used for providing liquid path support for the sampling distribution module, the blood sedimentation detection module and the blood routine detection module;
the sampling device also comprises a sample sucking pipeline which is connected with the sampling needle and the first power device; the liquid path support module is connected to the sample suction pipeline, at least part of the sample suction pipeline is filled with diluent, and after the sampling needle collects a blood sample, an isolation gas column is arranged between the blood sample and the diluent.
2. The blood analyzer of claim 1,
the volume of the isolated gas column is more than or equal to 5 mu L and less than or equal to 20 mu L.
3. The blood analyzer of claim 2,
the volume of the isolated gas column is more than or equal to 10 mu L and less than or equal to 15 mu L.
4. The blood analyzer of claim 1,
the detection pipeline is a part of the sample suction pipeline, and the optical detection device is arranged on two sides of the sample suction pipeline; the sample separation device is connected to the sample suction pipeline, and after sampling of the sampling needle is completed, the sample separation device sucks at least part of the blood sample close to the isolated gas column to the detection pipeline.
5. The blood analyzer of claim 4,
the volume distance between the detection pipeline and the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 mu L, and the preset volume distance is the sum of the preset maximum sample suction amount and the volume of the isolation gas column.
6. The blood analyzer of claim 5,
the blood sedimentation detection module further comprises a heater, the heater is used for heating the blood sample in the detection pipeline, the distance from the heater to the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 mu L, and the preset volume distance is the sum of a preset maximum sample suction amount and the volume of the isolation gas column.
7. The blood analyzer of claim 1,
the blood analyzer also comprises a controller, wherein the controller controls the sample separation device to firstly distribute a first part of blood sample close to the needle point of the sampling needle to the blood routine detection pool and then distribute a second part of blood sample close to the isolated gas column to the blood sedimentation measurement module.
8. The blood analyzer of claim 1,
the detection pipeline is connected to the sample suction pipeline; the sample separation device is connected to the detection pipeline, and after sampling of the sampling needle is completed, the sample separation device sucks at least part of the blood sample close to the isolated gas column to the detection pipeline.
9. The blood analyzer of claim 8,
the volume distance between the detection pipeline and the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 mu L, and the preset volume distance is the sum of the preset maximum sample suction amount and the volume of the isolation gas column.
10. The blood analyzer of claim 1,
the blood routine detection pool is connected to the sample suction pipeline, the sample separation device is connected to the blood routine detection pool, and after sampling of the sampling needle is completed, the sample separation device sucks at least a part of the blood sample close to the isolated gas column to the blood routine detection pool; the volume distance between the blood routine detection pool and the needle point of the sampling needle is greater than a preset volume distance and less than or equal to 200 mu L, and the preset volume distance is the sum of the preset maximum sample suction amount and the volume of the isolation gas column.
11. A blood analyzer, comprising:
the sampling distribution module comprises a sampling device for collecting blood samples, the sampling device comprises a sampling needle and a first power device, the first power device is used for driving the sampling needle to collect the blood samples, and the sample distribution device is used for distributing the collected blood samples to different detection modules;
the erythrocyte sedimentation rate detection device comprises a blood sedimentation detection module and a control module, wherein the blood sedimentation detection module comprises a detection pipeline and an optical detection device, the detection pipeline provides a detection place for a blood sample, and the optical detection device is used for carrying out light irradiation on the blood sample in the detection pipeline and detecting the absorption or scattering degree of the blood sample in the detection pipeline to the light so as to obtain the erythrocyte sedimentation rate of the blood sample;
the blood routine detection module comprises a blood routine detection pool and a blood routine detection device, the blood routine detection pool provides a detection place for a blood sample, and the blood routine detection device is used for performing blood routine detection on the blood sample in the blood routine detection pool;
the detection pipeline is connected to the sampling needle, and the detection pipeline is statically arranged relative to the sampling needle in at least one movement direction.
12. The blood analyzer of claim 11,
the detection pipeline is arranged on the sampling distribution module.
13. The blood analyzer of claim 12,
the sampling dispensing module comprises a motion assembly to which the sampling needle is mounted and a drive means for driving the motion assembly and the sampling needle in a predetermined direction; the detection pipeline is arranged on the motion assembly.
14. The blood analyzer of claim 13,
the motion assembly comprises a transverse motion assembly and a vertical motion assembly which are driven by the driving device to respectively move transversely and vertically, and the detection pipeline is arranged on the transverse motion assembly or the vertical motion assembly.
15. The blood analyzer of claim 12,
the blood sedimentation detection module is arranged on the sampling distribution module.
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| CN2020107657857 | 2020-07-31 | ||
| CN202010765785 | 2020-07-31 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166259A (en) * | 2022-07-12 | 2022-10-11 | 深圳赛斯鹏芯生物技术有限公司 | Whole blood protein detector combining blood cell classification and counting and detection method thereof |
| CN119178893A (en) * | 2024-11-26 | 2024-12-24 | 深圳市帝迈生物技术有限公司 | A blood cell combined detection analyzer |
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2021
- 2021-07-30 CN CN202110878480.1A patent/CN114062209A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115166259A (en) * | 2022-07-12 | 2022-10-11 | 深圳赛斯鹏芯生物技术有限公司 | Whole blood protein detector combining blood cell classification and counting and detection method thereof |
| CN119178893A (en) * | 2024-11-26 | 2024-12-24 | 深圳市帝迈生物技术有限公司 | A blood cell combined detection analyzer |
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