CN115166259A

CN115166259A - Whole blood protein detector combining blood cell classification and counting and detection method thereof

Info

Publication number: CN115166259A
Application number: CN202210816845.2A
Authority: CN
Inventors: 易容华; 蓝储; 张家忠; 王茂房; 王山; 李宗奇
Original assignee: Shenzhen Saisi Pengxin Biotechnology Co ltd
Current assignee: Shenzhen Saisi Pengxin Biotechnology Co ltd
Priority date: 2022-07-12
Filing date: 2022-07-12
Publication date: 2022-10-11

Abstract

The application relates to the field of blood analyzers, in particular to a blood cell differential counting combined whole blood protein detector and a detection method thereof, wherein the blood cell differential counting combined whole blood protein detector comprises a rack, a sample pre-storage position, a whole blood sample adding position, a blood routine examination position, a reagent storage area, a transfer sample position and a protein detection position are arranged on the rack; a plurality of reaction cups; the rotating platform is used for placing each reaction cup and is used for driving each reaction cup to move according to a preset circumferential carrying path, and the circumferential carrying path passes through the protein detection position, the whole blood sample adding position and the sample transferring position; a whole blood sample needle; and the whole blood carrying unit is arranged on the rack. For a whole blood sample in the same sample test tube, the serum specific gravity of the blood can be obtained through routine blood examination, and in protein detection, error correction can be carried out according to the serum specific gravity, so that a more accurate protein detection result is obtained. The method and the device have the effect of reducing detection errors.

Description

Whole blood protein detector combining blood cell classification and counting and detection method thereof

Technical Field

The application relates to the field of blood analyzers, in particular to a blood cell classification and counting combined whole blood protein detector and a detection method thereof.

Background

A blood cell analyzer is an instrument for detecting a specific protein by examining a blood sample. Specific proteins that can be detected by a hemocyte analyzer generally include C-reactive protein (CRP, an acute phase protein synthesized by hepatocytes) and amyloid a (SAA, an acute phase protein secreted into serum after being produced by hepatocytes).

In clinical examination, after a blood sample of a patient is collected, the blood sample can be analyzed by a blood cell analyzer to detect specific proteins, and the detection result of the specific proteins is helpful for the patient to judge the condition of the patient. Wherein the detection result of the C-reactive protein is specific to the condition of bacterial infection, and the detection result of the serum amyloid protein A is specific to the condition of viral infection. However, the specific protein is present only in human serum, and there is a problem that a detection error is large when the specific protein is directly detected in whole blood.

Disclosure of Invention

In order to reduce detection errors, the application provides a blood cell differential counting combined whole blood protein detector and a detection method thereof.

In a first aspect, the present application provides a blood cell differential count and whole blood protein combined detector, which adopts the following technical scheme:

a kind of blood cell differential count unites the whole blood protein detector, including: a rack provided with a sample pre-storage position for placing a sample test tube, a whole blood sample adding position for adding a whole blood sample into a container, a blood routine inspection position for performing blood routine inspection on the whole blood sample, a reagent storage area for placing a protein reagent, a transfer sample position for adding the protein reagent into the container, and a protein detection position for performing protein detection; the reaction cups are divided into a blood dissolving cup and a detection cup; the rotating platform is arranged on the rack, is used for placing each reaction cup and is used for driving each reaction cup to move according to a preset circumferential carrying path, and the circumferential carrying path passes through the protein detection position, the whole blood sample adding position and the transfer sample position; a whole blood sample needle for loading the whole blood sample into the hemolysis cup; a whole blood carrying unit, disposed on the rack and connected to the whole blood sample needle, for moving the whole blood sample needle according to a preset whole blood carrying path, wherein the whole blood carrying path passes through the sample pre-storage position, the whole blood loading position and the blood routine checking position, and the whole blood carrying path intersects with the circumferential carrying path at the whole blood loading position; a transfer sample needle for loading the sample in the cuvette into the cuvette and adding the reagent in the reagent storage section to the cuvette; and the sample transfer unit is arranged on the frame, is connected with the transfer sample needle, and is used for driving the transfer sample needle to move according to a preset transfer carrying path, the transfer carrying path passes through the reagent storage area and the transfer sample position, and the transfer carrying path and the circumferential carrying path are intersected at the transfer sample position.

Optionally, the detecting cup is divided into a first detecting cup and a second detecting cup, and the reagent storing area includes a first protein reagent position corresponding to the first detecting cup and a second protein reagent position corresponding to the second detecting cup.

Optionally, each of the detection cups is circumferentially distributed around the rotating platform, each of the hemolyzing cups and each of the detection cups are alternately distributed, and each of the first detection cups and each of the second detection cups are alternately distributed.

Optionally, the reagent storage zone comprises a diluted reagent site, and the diluted reagent site, the first protein reagent site, the second protein reagent site and the transfer sample site are spaced apart along an arcuate path.

Optionally, the whole blood carrying path is distributed along a line tangent to the circumferential carrying path, and the whole blood loading position is located at a tangent position.

In a second aspect, the present application provides a detection method, which adopts the following technical solution:

a test method implemented by the apparatus for differential blood cell count in combination with a whole blood protein test apparatus according to the present invention comprises: aspirating a whole blood sample from the sample pre-storage location through the whole blood carrying unit and the whole blood sample needle; loading the whole blood sample into the cuvette at the whole blood loading position through the rotary platform, the whole blood carrying unit, and the whole blood sample needle; and executing corresponding detection tasks based on the current detection items, wherein the detection tasks comprise a blood routine examination task and a protein detection task, and the protein detection task comprises a single protein detection task and a multiple protein detection task.

Optionally, the step of executing the corresponding detection task based on the current detection item includes: moving the whole blood sample needle to the blood routine testing site by the whole blood carrying unit; loading the whole blood sample in the hemolysis cup into a detection cup of the transfer sample site through the rotary platform and the transfer sample needle; adding the protein reagent to the test cup of the transfer sample site; and moving the detection cup to the protein detection position for protein detection.

Optionally, the detection cup is divided into a first detection cup and a second detection cup, and the first detection cup and the second detection cup are used for performing different protein detections on the same whole blood sample;

the step of loading the whole blood sample in the hemolysis cup into the test cup in the transfer sample position via the rotary platform and the transfer sample needle comprises: the rotary platform moves the hemolysis cup to the transfer sample position, the transfer sample needle aspirating a first designated volume of whole blood sample in the hemolysis cup; the rotating platform moves the first test cup to the transfer sample position, and the transfer sample needle loads a first designated volume of the whole blood sample into the first test cup; the rotary platform moves the hemolysis cup to the transfer sample position, the transfer sample needle aspirating a second specified volume of whole blood sample in the hemolysis cup; the rotating platform moves the second test cup to the transfer sample position, and the transfer sample needle applies a second designated volume of the whole blood sample to the second test cup.

Optionally, the detection cup is divided into a first detection cup and a second detection cup, and the first detection cup and the second detection cup are used for performing different protein detections on the same whole blood sample; the rotary platform moves the hemolysis cup to the transfer sample position, the transfer sample needle aspirating a third designated volume of whole blood sample in the hemolysis cup; the rotating platform moves the first test cup to the transfer sample position, and the transfer sample needle loads a first designated volume of whole blood sample into the first test cup; the rotary platform moves the second test cup to the transfer sample position, and the transfer sample needle applies a second designated volume of the whole blood sample to the second test cup.

Optionally, the third specified volume is greater than the sum of the first specified volume and the second specified volume.

According to the blood cell classification and counting combined whole blood protein detector and the detection method thereof, when protein detection is required, the whole blood carrying unit can also drive the whole blood sample needle to move to the blood routine examination position for blood routine examination, so that a detection result of the blood routine examination is obtained. For a whole blood sample in the same sample test tube, the serum specific gravity of the blood can be obtained through routine blood examination, and in protein detection, error correction can be carried out according to the serum specific gravity, so that a more accurate protein detection result is obtained, and detection errors are reduced. On the other hand, the whole blood sample needle can add the appearance in whole blood application of sample position and blood routine inspection position in proper order after inhaling the appearance in the sample test tube, then carries out blood routine inspection and protein detection again, and whole process only need inhale the appearance once in the sample test tube, has alleviated the problem that the whole speed that many times inhale the appearance and bring descends to it leads to the rubber lid of sample test tube to break to cause the risk of blood leakage to inhale the appearance many times to have reduced.

Drawings

FIG. 1 is a schematic diagram illustrating an appearance of a differential blood cell count and whole blood protein meter according to an embodiment of the present disclosure;

FIG. 2 is a top view of the internal structure of the differential blood cell count in combination with the whole blood protein meter according to the embodiment of the present application;

FIG. 3 is a simplified conceptual diagram of the structure of FIG. 2;

FIG. 4 depicts a conceptual schematic of a whole blood carrying pathway, a circumferential carrying pathway, and a transfer carrying pathway of an embodiment of the present application, where dashed line a indicates the whole blood carrying pathway, dashed line b indicates the circumferential carrying pathway, and dashed line c indicates the transfer carrying pathway;

FIG. 5 is a schematic view of a rotary platform;

FIG. 6 is a conceptual diagram illustrating the detection process of the complete blood protein monitor, wherein FIG. 6 (A) shows the CRP protein detection and the SAA protein detection performed simultaneously by the complete blood protein monitor, and FIG. 6 (B) shows the CRP protein detection performed by the complete blood protein monitor alone;

FIG. 7 is a schematic diagram showing the arrangement of the numbers of all reaction cups according to the embodiment;

FIG. 8 is a schematic diagram illustrating an internal structure of a combined complete blood protein and differential blood count detector according to an embodiment of the present application;

FIG. 9 is a schematic flow chart illustrating a detection method according to an embodiment of the present application;

FIG. 10 is a conceptual diagram illustrating the process of the detection method according to the embodiment of the present application;

FIG. 11 is a schematic diagram showing a process for performing a single-protein detection task in the detection method according to the embodiment of the present application;

FIG. 12 is a schematic view showing a process for performing a multi-protein detection task in the detection method according to the embodiment of the present application;

FIG. 13 is a schematic sub-flow chart of step B12 according to one embodiment of the present application;

fig. 14 is a sub-flowchart of step B12 in another embodiment of the present application.

Description of reference numerals:

1. a frame; 11. a sample pre-memory bit; 12. whole blood sample adding position; 13. a blood routine examination site; 13a, a leucocyte counting pool; 13b, a red blood cell counting pool; 14. a reagent storage area; 141. a first protein reagent site; 142. a second protein agent site; 143. a first buffer reagent site; 144. a second buffer reagent site; 145. diluting the reagent site; 146. transferring the cleaning position; 15. transferring the sample position; 16. a protein detection site; 161. a protein detection unit; 17. a vessel cleaning zone; 18. a transfer tray; 2. a reaction cup; 21. a blood lysing cup; 22. detecting the cup; 221. a first detection cup; 222. a second detection cup; 3. a whole blood sample needle; 4. transferring a sample needle; 5. rotating the platform; 51. a base; 52. a rotating frame; 6. a whole blood carrying unit; 7. a sample transfer unit; 8. a container cleaning assembly; 9. a machine body shell; 91. an observation window; 92. a touch screen type operation panel.

Detailed Description

At present, common routine blood examination (such as white blood cell count, white blood cell classification, red blood cell count, platelet count and the like) is detected on a traditional blood cell counter, so that a detection result can be quickly obtained, and the clinical diagnosis requirement is met (the patient is reported in 30 minutes). However, after the human body is infected with bacteria, viruses or mixed infection with bacteria and viruses, the change can be carried out in 2-3 days, and before the change, the condition of the human body is difficult to accurately judge by a clinician depending on the result of a blood routine examination alone, so that a cell immune examination is required to be combined.

When a human body is infected by bacteria, viruses or mixed bacteria and viruses, specific proteins in the human body are increased at the early stage of infection, the specific proteins generally comprise CRP aiming at the bacterial infection and SAA aiming at the viral infection, CRP is increased at the early stage of the bacterial infection, and SAA is increased at the early stage of the viral infection. In the protein detection in the cellular immunoassay, an antibody against CRP or an antibody against SAA may be added to blood to perform an antigen-antibody reaction, thereby obtaining a detection result of the above-mentioned specific protein. The clinician can more accurately judge the disease condition by combining the detection result of the specific protein (such as the detection result of CRP, the detection result of SAA) and the detection result of the blood routine examination.

The above specific proteins are present only in human serum, and there are two main methods for blood analysis of characteristic proteins:

1. the clinical laboratory in hospital firstly centrifuges a batch of whole blood samples into serum, and then uniformly detects the serum on an immunoassay analyzer or a blood analyzer to obtain a protein detection result. The method needs 4-6 hours to obtain results in the whole process, has low efficiency and seriously delays the efficiency of doctors.

2. The serum volume ratio (also called serum specific gravity, namely the ratio of serum to whole blood volume) of the patient is estimated through prediction, and then protein detection is carried out on the whole blood sample of the patient based on the preset serum volume ratio to obtain a detection result. Because different people have different serum volume ratios (normal people account for 40-60%), the method has errors in serum volume, improves the detection efficiency but sacrifices the detection precision.

Based on the defects of the prior art method, the application provides a blood cell classification and counting combined whole blood protein detector, aiming at reducing the detection error of protein detection, improving the whole time of analysis and detection and improving the detection efficiency.

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Embodiments of the present invention are described in further detail below with reference to figures 1-14 of the specification.

The embodiment of the application discloses a whole blood protein detector is united in blood cell differential count.

Referring to fig. 1 and 2, the combined complete blood protein monitor for differential blood count includes a frame 1 and a body housing 9 covering the frame 1, wherein an observation window 91 and a touch screen type operation panel 92 are disposed on the upper portion of the body housing 9.

Referring to fig. 2 and 3, the combined hemoglobin meter further comprises a plurality of cuvettes 2, a whole blood sample needle 3 for loading a whole blood sample into a container, a sample transfer needle 4 for adding reagents for analysis into the container and transferring the sample between the two containers, a rotary platform 5 disposed on the rack 1 for moving each cuvette 2, a whole blood carrier unit 6 disposed on the rack 1 for moving the whole blood sample needle 3, and a sample transfer unit 7 disposed on the rack 1 for moving the sample transfer needle 4. In the present embodiment, the container is referred to as a reaction cup 2, and the reaction cup 2 is divided into two types, namely a hemolyzing cup 21 and a detecting cup 22, wherein the hemolyzing cup 21 is used for storing a blood sample, and the detecting cup 22 is used for storing a sample of which blood reacts with protein antibodies.

Referring to fig. 2 and 3, in particular, the rack 1 is provided with a sample pre-storage location 11, and the sample pre-storage location 11 is used for placing a sample test tube. The sample tube contains a whole blood sample, which is a blood sample collected from a patient, and the whole blood sample in this embodiment does not need to be subjected to a serum extraction process such as a serum centrifugation process.

Referring to fig. 3 and 4, in the present embodiment, the whole blood carrying unit 6 moves the whole blood sample needle 3 in accordance with a preset whole blood carrying path (indicated by a dotted line a in fig. 4) which passes through the sample pre-storage location 11. When the whole blood carrying unit 6 carries the whole blood sample needle 3 to the sample pre-storage location 11, the whole blood sample needle 3 may aspirate a whole blood sample in a sample tube at the sample pre-storage location 11.

Referring to fig. 3 and 4, in this embodiment, the sample pre-storage location 11 is located inside the apparatus for classifying and counting blood cells, and the external operating platform of the apparatus for classifying and counting blood cells is provided with a conveying tray 18, a plurality of sample test tubes for analysis in the same batch can be placed on a prefabricated test tube rack, and when the test tube rack is placed on the conveying tray 18 according to a specified direction, the conveying tray 18 can drive the test tube rack to move, so that the test tube rack passes through the sample pre-storage location 11, so that each sample test tube on the test tube rack can enter the sample pre-storage location 11 in sequence and stay for a preset time, and the whole blood sample needle 3 can suck a sample of whole blood.

Referring to fig. 3 and 4, specifically, the rack 1 is further provided with a whole blood sample application position 12, and the whole blood sample application position 12 is used for the whole blood sample needle 3 to apply the whole blood sample which has been sucked into the hemolysis cup 21.

Referring to fig. 3 and 4, in the present embodiment, the whole blood carrying path passes through the whole blood loading position 12; the rotary platform 5 drives each of the blood dissolving cups 21 and each of the detecting cups 22 to move according to a preset circular carrying path (shown by a dotted line b in fig. 4), and the circular carrying path also passes through the whole blood sample adding position 12, i.e. the circular carrying path and the whole blood carrying path intersect at the whole blood sample adding position 12. When the whole blood carrying unit 6 drives the whole blood sample needle 3 to move to the whole blood sample adding position 12, and the rotating platform 5 drives one of the blood dissolving cups 21 to move to the whole blood sample adding position 12, the whole blood sample needle 3 can add the whole blood sample to the blood dissolving cup 21 located at the whole blood sample adding position 12.

Referring to fig. 3 and 4, specifically, a blood routine test site 13 is further disposed on the rack 1, and the blood routine test site 13 is provided with a blood routine test unit, which can perform a blood routine test on the whole blood sample to obtain a serum volume ratio of the whole blood sample, wherein the test time is approximately 1 minute. In the present embodiment, the blood routine-testing unit includes a white blood cell count cell 13a for calculating the white blood cell content in blood and a red blood cell count cell 13b for calculating the red blood cell content in blood.

Referring to fig. 3 and 4, in the present embodiment, the whole blood carrying path passes through the blood routine test site 13. When the whole blood carrying unit 6 drives the whole blood sample needle 3 to move to the blood routine inspection position 13, the whole blood sample needle 3 can load the whole blood sample into the blood routine inspection unit of the blood routine inspection position 13, that is, the whole blood sample is sequentially loaded into the white blood cell counting cell 13a and the red blood cell counting cell 13b, so as to obtain the serum volume ratio of the whole blood sample.

Referring to fig. 3 and 4, specifically, the rack 1 is further provided with a reagent storage area 14, and several analysis reagents including a protein reagent containing an antibody for an antigen-antibody reaction in a specific protein assay are disposed in the reagent storage area 14, and in this embodiment, the protein reagent includes a CRP antibody reagent corresponding to a CRP protein assay and an SAA antibody reagent corresponding to an SAA protein assay. Correspondingly, the reagent storage zone 14 includes a first protein reagent site 141 for the CRP antibody reagent and a second protein reagent site 142 for the SAA antibody reagent. Furthermore, the protein reagents of this example were all stored in a refrigerated environment, such as in an environment of 2-8℃, to maintain the effectiveness of the antibodies. In addition, the reagents for analysis mentioned in the present example are contained in appropriate reagent bottles.

Referring to fig. 3 and 4, in the present embodiment, the sample transfer unit 7 moves the transfer sample needle 4 in accordance with a preset transfer carrying path (indicated by a broken line c in fig. 4) that passes through the reagent storage area 14. When the sample transfer unit 7 moves the transfer sample needle 4 to the reagent storage area 14, the transfer sample needle 4 can aspirate a corresponding reagent for analysis, such as a CRP antibody reagent or a SAA antibody reagent, in the reagent storage area 14.

Referring to fig. 3 and 4, specifically, the rack 1 is further provided with a transfer sample position 15, the transfer sample position 15 is used for the transfer sample needle 4 to add the aspirated reagent for analysis into the detection cup 22, and the transfer sample needle 4 is used for adding the whole blood sample in the blood dissolving cup 21 into the corresponding detection cup 22.

Referring to fig. 3 and 4, in the present embodiment, the transfer carrying path passes through the transfer sample site 15, and the circumferential carrying path also passes through the transfer sample site 15, i.e., the transfer carrying path and the circumferential carrying path intersect at the transfer sample site 15.

Referring to fig. 3 and 4, when a reagent for analysis needs to be added to the cuvette 21 or the cuvette 22, and a protein reagent needs to be added to the corresponding cuvette 22, the sample transfer unit 7 moves the transfer sample needle 4 to the transfer sample site 15, and the rotary platform 5 moves the corresponding cuvette 22 to the transfer sample site 15, and the transfer sample needle 4 adds the protein reagent to the cuvette 22.

Referring to fig. 3 and 4, when the whole blood sample in the blood dissolving cup 21 needs to be transferred to the detection cup 22 that needs to perform antigen-antibody reaction, the rotary platform 5 first drives the blood dissolving cup 21 to move to the transfer sample position 15, the transfer sample needle 4 can suck the whole blood sample in the blood dissolving cup 21, then the rotary platform 5 drives the blood dissolving cup 21 to move out of the transfer sample position 15 and drives the corresponding detection cup 22 to move to the transfer sample position 15, and the transfer sample needle 4 adds the whole blood sample to the detection cup 22.

Referring to fig. 3 and 4, specifically, the rack 1 is further provided with a protein detection site 16, and the protein detection site 16 is provided with a protein detection unit 161. The protein detection site 16 coincides with the whole blood sample application site 12. In this embodiment, the protein detecting unit 161 employs a protein optical module, which is a laser bar and a receiving plate, when the detecting cup 22 reaches the protein detecting position 16, the laser bar provides a light source, and the light source passes through the detecting cup 22 to form a scattered light signal, which is received by the receiving plate, so as to help the instrument to convert the sample concentration. In the present embodiment, the lateral interface of the detecting cup 22 is rectangular, and the detecting cup 22 is made of transparent material.

Referring to fig. 3 and 4, in this embodiment, the circumferential carrying path passes through the protein detection site 16. When the rotary platform 5 drives the detection cup 22 which has undergone antigen-antibody reaction to move to the protein detection position 16, the protein detection unit 161 detects the sample in the detection cup 22, and obtains a detection result.

Referring to fig. 3 and 4, as to the detailed description of the whole blood carrying unit 6, in this embodiment, the whole blood carrying unit 6 is preferably a linear guide mechanism driven by a motor, and the whole blood carrying path is distributed along a straight line on which the sample pre-storage position 11, the whole blood sample application position 12, the white blood cell 13a, and the red blood cell 13b are distributed in order. The whole blood carrying unit 6 drives the whole blood sample needle 3 to move along the whole blood carrying path, and actually drives the whole blood sample needle 3 to make a linear motion or a linear reciprocating motion along a guide rail with a specified volume.

Referring to fig. 3 and 5, as to a detailed description of the rotating platform 5, in the present embodiment, the rotating platform 5 includes a base 51 fixed to the frame 1, a rotating frame 52 rotatably connected to an upper portion of the base 51, and a driving motor (not shown) disposed between the base 51 and the rotating frame 52, and each reaction cup 2 is placed on the rotating frame 52, and the driving motor is used for driving the rotating frame 52 to rotate. All the reaction cups 2 are circumferentially spaced on the rotating frame 52, and the circular axis surrounded by each reaction cup 2 overlaps with the rotating axis of the rotating frame 52. The rotating platform 5 drives each reaction cup 2 to move along the circular transportation path, and actually the rotating frame 52 drives each reaction cup 2 to make a circular motion at the same time.

Referring to fig. 3 and 4, in the present embodiment, the whole blood carrying unit 6 is located at one side edge portion of the rotating platform 5, the straight track of the whole blood carrying unit 6 overlaps with the peripheral edge of the rotating platform 5 in the vertical direction, i.e., the whole blood carrying path is tangent to the circumferential transport path, and the whole blood sample application site 12 is formed at the tangent position.

Referring to fig. 3, in the present embodiment, the whole blood carrying unit 6 is further configured with a lifting driving module, when the whole blood sample needle 3 performs a sample sucking or sample adding operation, the lifting driving module drives the whole blood sample needle 3 to descend to a specified volume height, and drives the whole blood sample needle 3 to ascend to the specified volume height after the sample sucking or sample adding operation is completed.

Referring to fig. 5, a detailed description of the cuvette 2 is provided, in this embodiment, the cuvette 2 is divided into a hemolyzing cup 21 and a detecting cup 22, wherein the detecting cup 22 is further divided into a first detecting cup 221 and a second detecting cup 222, and the first detecting cup 221 and the second detecting cup 222 are respectively used for different protein detecting items. In all the cuvettes 2 configured with the complete blood protein meter in combination with the differential blood cell count, the number of the hemolysis cups 21 is greater than or equal to 1, the number of the first detection cups 221 is greater than or equal to 1, and the number of the second detection cups 222 is greater than or equal to 1.

Referring to fig. 6 (a), in the present embodiment, the blood cell differential count and whole blood protein meter provides the functions of CRP protein detection and SAA protein detection, if the CRP protein detection and the SAA protein detection are required to be performed simultaneously, the first cup 221 is default to a designated volume for CRP protein detection, and the protein reagent added to the first cup 221 is default to the CRP antibody reagent; the second detection cup 222 is default designated volume for SAA protein detection, and the protein reagent added to the second detection cup 222 is default to SAA antibody reagent. In the above-mentioned testing process, in order to ensure that the sources of the whole blood samples are consistent, the whole blood sample added into the first testing cup 221 and the whole blood sample added into the second testing cup 222 are both from the same hemolysis cup 21, that is, there is a correlation relationship between the two testing cups 22 and one hemolysis cup 21.

Referring to fig. 6 (B), it can be understood that if only one protein assay is required, such as only CRP protein assay or only SAA protein assay, the first assay cup 221 and the second assay cup 222 can be simultaneously designated as volumes for CRP protein assay or SAA protein assay, and there is an interrelated relationship between one assay cup 22 and one lysis cup 21 during the above assay.

Referring to fig. 5, further, each reaction cup 2 has a cup number, and the reaction cups 2 are sequentially arranged around the rotating platform 5 in the order of the cup numbers from small to large. In all the reaction cups 2, the respective hemolyzing cups 21 and the respective detecting cups 22 are alternately arranged, and the respective first detecting cups 221 and the respective second detecting cups 222 are alternately arranged.

Referring to fig. 7, in the present embodiment, the total number of the cuvettes 2 is 32, and the cuvettes 2 are sequentially arranged in the clockwise direction according to the respective cup numbers, wherein the cuvette 1 is the hemolyzing cup 21, the cuvette 2 is the first detecting cup 221, the cuvette 3 is the hemolyzing cup 21, the cuvette 4 is the second detecting cup 222, the cuvette 5 is the hemolyzing cup 21, the cuvette 6 is the first detecting cup 221, the cuvette 7 is the hemolyzing cup 21, the cuvette 8 is the second detecting cup 222, the cuvette 9 is the hemolyzing cup 21, \ 8230, and the cuvette 32 is the second detecting cup 222. Namely, the reaction cup 2 with the odd number of the cup body is the blood dissolving cup 21, and the reaction cup 2 with the even number of the cup body is the detecting cup 22.

Referring to fig. 6 and 7, in a manner that the blood dissolving cups 21 and the detecting cups 22 are adjacently and alternately arranged, when the CRP protein detection or the SAA protein detection needs to be performed, the whole blood sample needle 3 firstly adds the whole blood sample into one of the reaction cups 2, then the reaction cup 2 moves to the sample transfer position 15, the whole blood sample in the reaction cup 2 is sucked by the sample transfer needle 4, then the rotary platform 5 rotates, the reaction cup 2 is separated from the sample transfer position 15, the next detecting cup 22 moves to the sample transfer position 15, and then the whole blood sample is added into the detecting cup 22 by the sample transfer needle 4.

Referring to fig. 3 and 4, for example, if the whole blood sample needle 3 adds the whole blood sample to the No. 1 hemolyzing cup 21 first, the No. 1 hemolyzing cup 21 moves to the transfer sample position 15, the transfer sample needle 4 sucks the whole blood sample in the No. 1 hemolyzing cup 21, then the No. 2 detecting cup 22 moves to the transfer sample position 15, and the transfer sample needle 4 adds the whole blood sample to the No. 2 detecting cup 22; if the whole blood sample is added into the No. 3 hemolyzing cup 21 by the whole blood sample needle 3, the No. 3 hemolyzing cup 21 is moved to the transfer sample position 15, the whole blood sample in the No. 3 hemolyzing cup 21 is sucked by the transfer sample needle 4, then the No. 4 detecting cup 22 is moved to the transfer sample position 15, and the whole blood sample is added into the No. 4 detecting cup 22 by the transfer sample needle 4.

Referring to fig. 5 and 6, in the above-mentioned detection process, there is a correlation between one blood lysing cup 21 and the next detection cup 22 to form a set of reaction cups, i.e. a set of reaction cups (e.g. the No. 1 blood lysing cup 21 and the No. 2 detection cup 22) is formed between every 2 reaction cups 2 arranged in series, so as to obtain a protein detection result corresponding to a whole blood sample. If there are multiple sample tubes to be analyzed in the same batch of analysis operations, each sample tube corresponds to one set of reaction cup groups, and the sets of reaction cup groups are sequentially and continuously distributed around the rotating platform 5.

Referring to fig. 3 and 4, when the CRP protein test and the SAA protein test are performed simultaneously, the whole blood sample needle 3 may first add the whole blood sample into one of the reaction cups 2, then the reaction cup 2 moves to the transfer sample position 15, and the transfer sample needle 4 sucks the whole blood sample in the reaction cup 2 and adds the whole blood sample into the next first detection cup 221 and the next second detection cup 222, respectively.

Referring to fig. 3 and 4, for example, if the whole blood sample needle 3 adds a whole blood sample to the No. 1 hemolyzing cup 21 first, the transfer sample needle 4 sucks the whole blood sample in the No. 1 hemolyzing cup 21 and adds the whole blood sample to the No. 2 first detecting cup 221 and the No. 4 second detecting cup 222; if the whole blood sample needle 3 adds the whole blood sample to the sample cup 21, the transfer sample needle 4 sucks the whole blood sample from the sample cup 21 and adds the whole blood sample to the sample cups 221, 222.

Referring to fig. 5 and 6, in the above-mentioned detection process, there is a correlation relationship between one of the blood dissolving cups 21, the next first detection cup 221, the next first detection cup 21, and the next second detection cup 222 (e.g. the No. 1 blood dissolving cup 21, the No. 2 first detection cup 221, the No. 3 blood dissolving cup 21, and the No. 4 second detection cup 222, where the No. 3 blood dissolving cup 21 does not need to be loaded with the whole blood sample), so as to form a set of reaction cup groups, that is, a set of reaction cup groups is formed between every 4 reaction cups 2 arranged in series, so as to obtain two protein detection results corresponding to one whole blood sample.

Referring to fig. 5, if there are multiple sample tubes to be analyzed in the same batch of analysis operations, each sample tube corresponds to one set of reaction cup groups, and the multiple sets of reaction cup groups are sequentially distributed around the rotating platform 5.

In this embodiment, no matter a single detection item or a plurality of detection items, a plurality of groups of reaction cup groups are formed on the rotary platform 5, each group of reaction cup group is composed of a plurality of reaction cups arranged in series, which facilitates the algorithm system to perform statistics of the relevance between each reaction cup 2 and the sample test tube, and facilitates the collective cleaning of each group of reaction cup group.

Referring to fig. 5, it can be understood that the total number of reaction cups 2 in this embodiment is only one of the preferred examples, so that at most 8 reaction cup sets can perform analysis simultaneously, and the analysis efficiency is improved, in other embodiments, the total number of reaction cups 2 may be adjusted according to the actual application scenario and the amount of the analyte, as long as the total number of reaction cups 2 = N4, and N is greater than or equal to 1.

Referring to fig. 2 and 3, as to the concrete description of the sample transfer unit 7, in this embodiment, the sample transfer unit 7 is preferably a swing arm robot, and the swing arm of the swing arm robot drives the transfer sample needle 4 to make a circular motion, and the transfer carrying paths are distributed along an arc.

Referring to fig. 2 and 4, specifically, the sample transfer unit 7 is located in a direction of a side edge portion of the rotating platform 5 away from the whole blood carrying unit 6, the swing range of the sample transfer unit 7 has an overlapping portion with the peripheral edge of the rotating platform 5, i.e., the transfer carrying path is tangent to the circumferential transport path, and the transfer sample site 15 is formed at the tangent position.

Referring to fig. 2, in the present embodiment, the sample transfer unit 7 is further configured with a lifting driving module, when the transfer sample needle 4 performs a sample sucking or sample adding operation, the lifting driving module drives the transfer sample needle 4 to descend to a specified volume height, and drives the transfer sample needle 4 to ascend to the specified volume height after the sample sucking or sample adding operation is completed.

Referring to FIG. 3, as to the details of the reagent storage section 14, in this embodiment, the analytical reagent further includes a buffer solution for providing an environment of a suitable pH. The reagent storage section 14 includes not only the first protein reagent site 141 and the second protein reagent site 142, but also the first buffer reagent site 143 and the second buffer reagent site 144. The first buffer reagent site 143 stores a buffer solution corresponding to the detection of CRP protein, and the second buffer reagent site 144 stores a buffer solution corresponding to the detection of SAA protein. The first buffer reagent level 143 and the second buffer reagent level 144 each pass through the transfer carrying path so that the transfer sample needle 4 can add the buffer solution on the first buffer reagent level 143 or the second buffer reagent level 144 to the corresponding detection cup 22.

Referring to fig. 3, when protein detection is required, before the whole blood sample is added to the first detection cup 221, the transfer sample needle 4 needs to suck a corresponding buffer solution from the first buffer reagent site 143 and add the buffer solution to the first detection cup 221; second test cup 222 before the whole blood sample is added, transfer sample needle 4 needs to draw the corresponding buffer solution from second buffer reagent site 144 and add it to second test cup 222.

Referring to fig. 3, in the present embodiment, the analysis reagent further includes a hemolyzing diluent for hemolyzing and diluting blood. The reagent storage area 14 further comprises a diluted reagent site 145 for storing a hemolyzed diluent.

Referring to fig. 3, when protein detection is required, before the whole blood sample is added to the first detection cup 221, the transfer sample needle 4 needs to suck a hemolyzing diluent from the diluting reagent site 145 and add the hemolyzing diluent to the hemolyzing cup 21, and then the whole blood sample needle 3 adds the whole blood sample to the hemolyzing cup 21.

Referring to fig. 3, in the present embodiment, the rack 1 is further provided with a transfer cleaning station 146, a bottled cleaning liquid for cleaning the transfer sample needle 4 is stored in the transfer cleaning station 146, and the transfer cleaning station 146 passes through the transfer carrying path. After the sample transfer needle 4 finishes one sample application, for example, after the sample transfer needle 4 adds a reagent for analysis or a whole blood sample to the corresponding cuvette 2, the sample transfer unit 7 moves the sample transfer needle 4 to the transfer cleaning position 146, and the sample transfer needle 4 extends into the transfer cleaning position 146 for cleaning and then rises to the height of the designated volume after cleaning.

Referring to fig. 3, in the present embodiment, the housing 1 is further provided with a vessel cleaning section 17, and the vessel cleaning section 17 is provided with a vessel cleaning assembly 8 for cleaning the reaction cups 2. In this embodiment, the container washing section 17 is located at one side edge portion of the rotary platform 5, and the circumferential transport path passes through the container washing section 17. After a group of reaction cup groups complete all protein detection of a whole blood sample, the reaction cup groups move to the container cleaning area 17, the container cleaning component 8 cleans the reaction cup groups, and the reaction cup groups can participate in protein detection of the next whole blood sample after the cleaning is completed.

Referring to fig. 3, in the present embodiment, the container cleaning assembly 8 is composed of a plurality of cleaning members sequentially distributed along the rotation direction of the rotary platform 5, so that when each reaction cup 2 in a group of reaction cup groups can sequentially perform a cleaning operation, the cleaning members include, but are not limited to: a liquid outlet device used for extruding cleaning liquid, a brush head used for brushing the inner wall of the reaction cup 2 and a liquid suction device used for sucking away objects in the reaction cup 2.

The implementation principle of the detector combining blood cell classification and counting with whole blood protein in the embodiment of the application is as follows: the same whole blood sample can carry out blood routine inspection and protein detection respectively, obtain blood routine inspection result, protein detection result, and the serum volume ratio in the blood routine inspection result can carry out volume correction to the result in the protein detection, and then can obtain more accurate protein detection result, reduce detection error, and, this technical scheme does not need to carry out centrifugal treatment to the whole blood sample, and analysis speed is faster, improves analysis efficiency, can output protein detection result report fast, has great value to clinical examination.

Because the blood for routine blood examination and the blood for protein detection are both from the whole blood sample in the same sample test tube, the sample consistency of all detection items can be maintained, and the error is reduced. The routine blood inspection and the protein detection in the technical scheme are separately and independently carried out, the routine blood inspection and the protein detection are carried out in a parallel mode, namely, the routine blood inspection and the protein detection do not interfere with the detection speed of the other side, the whole blood sample for CRP protein detection and the whole blood sample for SAA protein detection are both from the blood dissolving cup 21, the sample adding of the whole blood sample needle 3 in the detection cup 22 is not needed, the whole process only needs the whole blood sample to be sucked in the sample test tube once, the problem of integral speed reduction caused by multiple times of sample sucking is relieved, and the risk of blood leakage caused by the breakage of a rubber cover of the sample test tube due to multiple times of sample sucking is reduced.

On the other hand, the technical scheme of the application has multiple detection modes, can independently perform blood routine examination, and can also perform blood routine examination plus CRP protein detection, blood routine examination plus SAA protein detection, or blood routine examination plus CRP protein detection plus SAA protein detection at the same time, so that the application range is wider.

The embodiment of the application also discloses a detection method, and the detection method is implemented by combining the blood cell classification and counting with the whole blood protein detector based on the technical scheme.

Referring to fig. 8 and 9, the detection method includes the steps of:

s1, sucking the whole blood sample from the sample pre-storage position 11 through the whole blood carrying unit 6 and the whole blood sample needle 3.

The whole blood carrying unit 6 moves the whole blood sample needle 3 to the sample pre-storage position 11, and a sample test tube to be analyzed and detected is stored in the sample pre-storage position 11; then, the whole blood sample needle 3 enters the sample tube to suck a designated volume of the whole blood sample. In this example, the specified volume for this step is 30 microliters.

S2, the whole blood sample is loaded into the hemolyzing cup 21 of the whole blood loading position 12 through the rotating platform 5, the whole blood carrying unit 6 and the whole blood sample needle 3.

For example, a specified volume of whole blood sample from the whole blood sample needle 3 is loaded into the number 1 cuvette 21 at the whole blood loading position 12, and the specified volume for this step is 10 microliters. The whole operation time is within 5 s. In this example, a 10 microliter whole blood sample added to the cuvette 21 may be subjected to the CRP protein test or SAA protein test in a subsequent step, while the remaining 20 microliter whole blood sample may be subjected to a blood routine test in a subsequent step.

Referring to fig. 8 and 10, step S2 specifically includes:

s21, adding a specified volume of hemolysis diluent into the hemolysis cup 21.

Wherein, the sample transferring unit 7 drives the transferring sample needle 4 to move to the diluted reagent position 145 first, and sucks the hemolyzed diluent; then, the sample transfer unit 7 drives the transfer sample needle 4 to move to the transfer sample position 15, and the rotary platform 5 drives the hemolyzing cup 21 to move to the transfer sample position 15; then, the transfer sample needle 4 adds the hemolyzed diluent to the hemolyzing cup 21. The hemolysis diluent can reduce the interference of factors such as hyperlipidemia and hypercholesterolemia on subsequent detection, and can relieve the viscosity of blood, so that the transfer sample needle 4 can accurately suck and sample in subsequent steps.

After this action is completed, the sample transfer unit 7 moves the transfer sample needle 4 to the transfer cleaning position 146 for cleaning.

For example, the transfer sample needle 4 adds 250. Mu.l of hemolyzed diluent to the No. 1 hemolysis cup 21 located at the transfer sample site 15.

S22, the rotating platform 5 moves the hemolyzing cup 21 to the whole blood sample adding position 12, the whole blood carrying unit 6 moves the whole blood sample needle 3 to the whole blood sample adding position 12, and the whole blood sample needle 3 adds the whole blood sample with the designated volume to the hemolyzing cup 21.

For example, the rotary platform 5 drives the number 1 hemolysis cup 21 to move to the whole blood sample loading position 12; the whole blood carrying unit 6 drives the whole blood sample needle 3 to move to the whole blood sample adding position 12, so that the whole blood sample needle 3 is positioned right above the No. 1 hemolysis cup 21; the whole blood sample needle 3 then loads 10 microliters of the whole blood sample into the number 1 cuvette 21.

It should be noted that if the CRP protein assay or SAA protein assay is not required in the subsequent operation, the hemolysis diluent may not be added into the hemolysis cup 21.

And S3, executing a corresponding detection task based on the current detection item.

The detection items refer to detection modes required by the current whole blood sample, and the detection items correspond to the detection tasks one to one. In this example, the test items include a blood routine test and a protein test, and the protein test includes a CRP protein test and a SAA protein test, and thus, the test items are three in total.

The detection tasks comprise a blood routine examination task and a protein detection task, and the protein detection task comprises a single protein detection task and a multiple protein detection task.

The blood routine task refers to performing a blood routine on a whole blood sample. The single protein detection task refers to CRP protein detection on a whole blood sample or SAA protein detection on a whole blood sample. The polyprotein detection task refers to CRP protein detection on whole blood samples and SAA protein detection on whole blood samples.

If the test items for the current whole blood sample include only blood routine tests, only the blood routine test task is performed.

If the current items of the test on the whole blood sample comprise a blood routine test and a CRP protein test, a blood routine test task and a single protein test task aiming at the CRP protein test are executed.

If the current items of the whole blood sample comprise a blood routine test and an SAA protein test, the blood routine test task and a single protein test task aiming at the SAA protein test are executed.

If the current items of the whole blood sample comprise a blood routine examination task and a multi-protein detection task, the blood routine examination task, the single-protein detection task aiming at the CRP protein detection and the single-protein detection task aiming at the SAA protein detection are executed.

It will be appreciated that, whether the testing task to be performed is one or a combination of two or more of the blood routine testing task, the single-protein testing task or the multi-protein testing task, the whole blood sample needle 3 will always draw the same volume (30 microliters in this embodiment) of whole blood sample in step S1, and the whole blood sample needle 3 will always add the same volume (10 microliters in this embodiment) of whole blood sample to the hemolysis cup 21 in step S2, and then perform the subsequent testing tasks.

Referring to fig. 8 and 10, in the present embodiment, a specific manner of performing the routine blood test task is as follows:

the whole blood sample needle 3 is moved to the blood routine test site 13 by the whole blood carrying unit 6 to perform a blood routine test.

The whole blood sampling needle 3 sequentially loads a specified volume of whole blood sample into the white blood cell count cell 13a and the red blood cell count cell 13b to obtain a serum volume ratio of the whole blood sample.

For example, a whole blood sample of 20 microliters is required for routine blood testing. Moving the whole blood sample to a white blood cell counting cell 13a, and adding 10 microliters of the whole blood sample to the white blood cell counting cell 13 a; the whole blood sample was moved to the red blood

cell count cell

13b, and 10. Mu.l of the whole blood sample was loaded into the red blood cell count cell 13 b.

Referring to fig. 8 and 11, in the present embodiment, the specific way to perform the single protein detection task is as follows:

a1, the whole blood sample in the blood dissolving cup 21 is loaded into the detection cup 22 of the transfer sample position 15 by rotating the platform 5 and the transfer sample needle 4.

The step A1 specifically comprises the following steps:

a11, adding a specified volume of buffer solution into the detection cup 22.

Wherein the sample transfer unit 7 moves the transfer sample needle 4 to the first buffer reagent position 143 or the second buffer reagent position 144 first and sucks the corresponding buffer solution, then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer sample position 15, and the rotary platform 5 drives the corresponding detection cup 22 to move to the transfer sample position 15, and the transfer sample needle 4 adds the buffer solution to the next adjacent detection cup 22.

When the CRP protein detection is performed, the transfer sample needle 4 needs to be aspirated from the first buffer reagent site 143 and then added to the corresponding detection cup 22.

For example, the transfer sample needle 4 sucks 120 microliters of the CRP buffer solution from the first buffer reagent site 143, and the No. 2 cuvette 22 moves to the transfer sample site 15, the transfer sample needle 4 adds the CRP buffer solution to the No. 2 cuvette 22, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer washing site 146 for washing.

When the SAA protein detection is performed, the transfer sample needle 4 needs to suck a sample from the second buffer reagent level 144 and then add the sample into the corresponding detection cup 22.

For example, the transfer sample needle 4 sucks 120 μ l of SAA buffer solution from the second buffer reagent site 144, and the No. 2 measuring cup 22 moves to the transfer sample site 15, the transfer sample needle 4 adds CRP buffer solution to the No. 2 measuring cup 22, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer washing site 146 for washing.

A12, a whole blood sample of a designated volume is loaded into the test cup 22 by rotating the platform 5 and transferring the sample needle 4.

Wherein, the rotary platform 5 moves the detecting cup 22 to the transferring sample position 15, the sample transferring unit 7 moves the transferring sample needle 4 to the transferring sample position 15, and the transferring sample needle 4 loads the whole blood sample with the designated volume into the detecting cup 22.

For example, in the case of CRP protein detection, the transfer sample needle 4 loads 20 microliters of whole blood sample into the No. 2 detection cup 22. In the SAA protein assay, the transfer sample needle 4 loads 20 microliters of whole blood sample into the test cup # 222.

A2, adding a protein reagent to the detection cup 22 of the transfer sample site 15.

Wherein the sample transfer unit 7 moves the transfer sample needle 4 to the reagent storage area 14, the transfer sample needle 4 aspirates a specified volume of the protein reagent in the reagent storage area 14, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer sample site 15. And, the rotary stage 5 moves the measuring cup 22 to the transfer sample position 15, and then the transfer sample needle 4 adds the protein reagent to the measuring cup 22.

Referring to fig. 8 and 10, in the CRP protein assay, the transfer sample needle 4 needs to be aspirated from the first protein reagent site 141 and then added to the assay cup 22.

For example, the transfer sample needle 4 sucks 40 microliters of the CRP antibody reagent from the first protein reagent site 141, and the No. 2 cuvette 22 moves to the transfer sample site 15, the transfer sample needle 4 adds the CRP antibody reagent to the No. 2 cuvette 22, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer washing site 146 for washing.

When performing SAA protein assay, the transfer sample needle 4 needs to aspirate from the second protein reagent site 142 and then add to the assay cup 22.

For example, the transfer sample needle 4 sucks 40 μ l of SAA antibody reagent from the second protein reagent site 142, and the No. 4 detection cup 22 moves to the transfer sample site 15, the transfer sample needle 4 adds the SAA antibody reagent to the No. 4 detection cup 22, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer cleaning site 146 for cleaning.

A3, the protein is detected by moving the cup 22 to the protein detection position 16.

Wherein, when the antigen-antibody reaction has been performed in the first detection cup 221 or the second detection cup 222 for a sufficient time, the corresponding first detection cup 221 or the second detection cup 222 moves to the protein detection position 16 for detection. Moreover, after a set of cuvettes 2 completes all protein detection of a whole blood sample, the set of cuvettes 2 moves to the container cleaning area 17, the container cleaning assembly 8 cleans the set of cuvettes 2, and the set of cuvettes 2 can participate in protein detection of the next whole blood sample after cleaning.

In this embodiment, the serum volume ratio in the blood routine examination result can be used for volume correction of the result in protein detection, so that a more accurate protein detection result can be obtained, and the detection error can be reduced.

It can be understood that, in the single protein detection task, there is a correlation relationship between one cuvette 21 and the next cuvette 22 to form a set of cuvette sets, that is, a set of cuvette sets (for example, cuvette 121 and cuvette 2) is formed between every 2 cuvettes 2 arranged in series for obtaining a protein detection result corresponding to a whole blood sample, and the cuvettes 22 are not necessarily divided into the first cuvette 221 and the second cuvette 222, and if there are a plurality of cuvettes to be analyzed in the same analysis operation, each cuvette corresponds to a set of cuvette sets, and the sets of cuvette sets are successively distributed around the rotary platform 5.

Referring to fig. 8 and 12, in the present embodiment, the specific way to perform the multi-protein detection task is:

b1, the whole blood sample in the blood dissolving cup 21 is loaded into the detection cup 22 of the transfer sample position 15 by rotating the platform 5 and the transfer sample needle 4.

The step B1 specifically includes:

b11, adding a specified volume of buffer solution into the detection cup 22.

Wherein, the sample transfer unit 7 moves the transfer sample needle 4 to the first buffer reagent position 143 or the second buffer reagent position 144 first and sucks the corresponding buffer solution, then, the sample transfer unit 7 moves the transfer sample needle 4 to the transfer sample position 15, and the rotary platform 5 drives the corresponding detection cup 22 to move to the transfer sample position 15, and the transfer sample needle 4 adds the buffer solution to the corresponding detection cup 22.

When the CRP protein test and the SAA protein test are performed simultaneously, the transfer sample needle 4 needs to sequentially aspirate from the first buffer reagent site 143 and the second buffer reagent site 144 and add them to the first test cup 221 and the second test cup 222, respectively.

For example, the transfer sample needle 4 sucks 120 μ l of the CRP buffer solution from the first buffer reagent site 143, and the No. 2 first cuvette 221 moves to the transfer sample site 15, the transfer sample needle 4 adds the CRP buffer solution to the No. 2 first cuvette 221, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer washing site 146 for washing. The transfer sample needle 4 sucks 120 microliters of SAA buffer solution from the second buffer reagent site 144, and the No. 4 second detection cup 222 moves to the transfer sample site 15, the transfer sample needle 4 adds the SAA buffer solution to the No. 4 second detection cup 222, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer washing site 146 for washing.

B12, loading the whole blood sample with the designated volume into the detection cup 22 by rotating the platform 5 and transferring the sample needle 4.

Wherein, the rotating platform 5 moves the detecting cup 22 to the transferring sample position 15, the sample transferring unit 7 moves the transferring sample needle 4 to the transferring sample position 15, and the transferring sample needle 4 loads the whole blood sample of the designated volume into the detecting cup 22.

When the CRP protein test and the SAA protein test are performed simultaneously, the transfer sample needle 4 needs to sequentially add the whole blood sample to the first test cup 221 and the second test cup 222.

For example, the transfer sample needle 4 loads 20 microliters of a whole blood sample into the first test cup No. 2 221, and then the transfer sample needle 4 loads 20 microliters of a whole blood sample into the second test cup No. 4 222.

Referring to fig. 8 and 13, in an embodiment, the specific implementation manner of step B12 is:

b121, the rotary platform 5 moves the hemolyzing cup 21 to the transferring sample position 15, and the transferring sample needle 4 sucks the first designated volume of the whole blood sample in the hemolyzing cup 21.

B122, the platform 5 is rotated to move the first detection cup 221 to the sample transferring position 15, and the sample transferring needle 4 loads the first designated volume of the whole blood sample into the first detection cup 221.

Wherein the suction amount of the whole blood sample in the step B121 is equal to the sample amount of the whole blood sample in the step B122. After this, the sample transfer unit 7 moves the transfer sample needle 4 to the transfer cleaning position 146 for cleaning.

B123, the rotary platform 5 moves the lysis cup 21 to the transfer sample position 15, and the transfer sample needle 4 sucks a second designated volume of whole blood sample in the lysis cup 21.

B124, rotating the platform 5 to move the second detection cup 222 to the sample transfer position 15, and the sample transfer needle 4 loads a second designated volume of whole blood sample into the second detection cup 222.

Wherein the amount of the whole blood sample sucked in the step B123 is equal to the amount of the whole blood sample added in the step B124. After this, the sample transfer unit 7 moves the transfer sample needle 4 to the transfer cleaning position 146 for cleaning.

For example, the transfer sample needle 4 aspirates 20 microliters of whole blood sample in the No. 1 cuvette 21, and then loads the whole 20 microliters of whole blood sample into the No. 2 first detection cup 221. Then, the transfer sample needle 4 sucks another 20. Mu.l of the whole blood sample in the No. 1 cuvette 21, and then the whole 20. Mu.l of the whole blood sample is loaded into the No. 4 second cuvette 222.

By using the technical scheme of the steps B121-B124, the transfer sample needle 4 performs sample suction from the hemolyzing cup 21 twice, and the whole blood sample in the transfer sample needle 4 is emptied each time, so that the suction amount required by the whole blood sample in a single protein detection can be stably controlled, and the sample waste is reduced.

On the other hand, because the blood itself has viscosity, the volume of the blood discharged by the transfer sample needle 4 in the completely emptied state and the remaining state is different, for example, the volume of the whole blood discharged by the transfer sample needle 4 in the state that the transfer sample needle itself sucks 20 microliters of the whole blood sample and discharges another 20 microliters of the whole blood sample (completely emptied) is different from the volume of the whole blood discharged by the transfer sample needle 4 in the state that the transfer sample needle itself sucks 30 microliters of the whole blood sample and discharges another 20 microliters of the whole blood sample (still 10 microliters remains), the former may be less than the latter, and there is a volumetric error. However, in the same series of algorithmic analyses, this error can be eliminated, and if all the transfer sample needles 4 are emptied to sample, all the input data have an error within a certain range, and this error can be uniformly calculated and eliminated in the later calculation; however, if the sample transfer needle 4 is used for sample loading in a partially emptying manner and in a partially remaining manner, an error in a certain range may exist in a portion of input data, and there is no error in the portion of input data, which may be difficult to eliminate in the later calculation, and thus the overall detection result of the apparatus is affected.

In the embodiment, the detection error can be effectively reduced on the basis of reducing sample waste by using a mode of sampling twice and emptying twice respectively.

Referring to fig. 8 and 14, in another embodiment, the specific implementation manner of step B12 is:

b1201, the rotating platform 5 moves the blood dissolving cup 21 to the transfer sample position 15, and the transfer sample needle 4 sucks a third designated volume of whole blood sample in the blood dissolving cup 21.

B1202, the platform 5 is rotated to move the first detection cup 221 to the sample transfer position 15, and the sample transfer needle 4 loads the first designated volume of the whole blood sample into the first detection cup 221.

B1203, the platform 5 is rotated to move the second detecting cup 222 to the sample transferring position 15, and the sample transferring needle 4 is used for adding a second designated volume of whole blood sample to the second detecting cup 222.

Wherein the third designated volume is larger than the sum of the first designated volume and the second designated volume, that is, the suction volume of the whole blood sample in step B1201 is larger than the sum of the sample adding volume of the whole blood sample in step B1202 and the sample adding volume of the whole blood sample in step B1203.

For example, the transfer sample needle 4 aspirates 45. Mu.l of a whole blood sample in the No. 1 cuvette 21, and then loads the whole 20. Mu.l of the whole blood sample into the No. 2 first measuring cup 221. Then, the transfer sample needle 4 further loads the whole 20 μ l of the whole blood sample into the second test cup No. 4 222.

By using the technical scheme of the steps B1201-B1203, when the whole blood sample in the blood dissolving cup 21 needs to be respectively added to the first detecting cup 221 and the second detecting cup 222, the transfer sample needle 4 may firstly suck a sufficient amount of whole blood sample in the blood dissolving cup 21, and then sequentially and respectively add the whole blood sample to the first detecting cup 221 and the second detecting cup 222. On one hand, the sample transferring needle 4 can finish two times of sample adding in sequence in subsequent actions through one-time sample sucking, and the sample adding efficiency is improved. On the other hand, since the transfer sample needle 4 can suck enough whole blood sample, the transfer sample needle 4 still has residual whole blood sample after the samples are loaded in the first detection cup 221 and the second detection cup 222 respectively, so as to eliminate the volume error between the first sample loading and the second sample loading, so that the accuracy of the sample loading amount of the transfer sample needle 4 is higher, and the detection accuracy is improved.

And B2, adding a protein reagent into the detection cup 22 of the transfer sample position 15.

Wherein the sample transfer unit 7 moves the transfer sample needle 4 to the reagent storage area 14, the transfer sample needle 4 aspirates a specified volume of the protein reagent in the reagent storage area 14, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer sample site 15. And, the rotation stage 5 moves the measuring cup 22 to the transfer sample position 15, and then the transfer sample needle 4 adds the protein reagent to the measuring cup 22.

When the CRP protein detection and the SAA protein detection are performed simultaneously, the transfer sample needle 4 needs to sequentially aspirate a sample from the first protein reagent site 141 and the second protein reagent site 142 and add the aspirated sample to the first measuring cup 221 and the second measuring cup 222, respectively.

For example, the transfer sample needle 4 sucks 40 microliters of the CRP antibody reagent from the first protein reagent site 141, and the No. 2 first cuvette 221 moves to the transfer sample site 15, the transfer sample needle 4 adds the CRP antibody reagent to the No. 2 first cuvette 221, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer wash site 146 for washing. The transfer sample needle 4 aspirates 40 microliters of SAA antibody reagent from the second protein reagent site 142, and the No. 4 second detection cup 222 moves to the transfer sample site 15, the transfer sample needle 4 adds the SAA antibody reagent to the No. 4 second detection cup 222, and then the sample transfer unit 7 moves the transfer sample needle 4 to the transfer cleaning site 146 for cleaning.

And B3, moving the detection cup 22 to the protein detection position 16 to detect the protein.

The implementation principle of the detection method in the embodiment of the application is as follows: the routine blood examination and the protein detection are separately and independently carried out, and the routine blood examination and the protein detection are carried out in a parallel mode, namely, the routine blood examination and the protein detection do not interfere with the detection speed of the other side, the whole blood sample for CRP protein detection and the whole blood sample for SAA protein detection are both from the blood dissolving cup 21, the sample adding of the whole blood sample needle 3 in the detection cup 22 is not needed, the whole process only needs one sample sucking of the whole blood sample in the sample test tube, the problem of integral speed reduction caused by multiple sample sucking is relieved, and the risk of blood leakage caused by the breakage of a rubber cover of the sample test tube due to multiple sample sucking is reduced.

On the other hand, in the actual operation, the preset sample adding amount and the actual sample adding amount of the whole blood sample needle 3 have errors, for example, in the case that the whole blood sample needle 3 first sucks a 30 μ l whole blood sample and then sequentially discharges 10 μ l and 20 μ l whole blood samples, the actual sequentially discharged amounts are not 10 μ l and 20 μ l, and there is an error, but if this error is fixed, it can be uniformly eliminated in the subsequent calculation; in the case where the whole blood sample needle 3 aspirates 20. Mu.l of the whole blood sample first and then discharges 20. Mu.l of the whole blood sample, there is an error in that the discharged amount is not 20. Mu.l, but if this error is fixed, it can be uniformly eliminated in the subsequent calculation.

In the technical scheme of the application, various detection tasks can be executed, and actually, the whole blood samples subsequently participating in the reaction may be different, for example, only 20 microliters of whole blood samples are needed for executing the blood routine examination task independently, only 10 microliters of whole blood samples are needed for executing the polyprotein detection task independently, and 30 microliters of whole blood samples are needed for executing the blood routine examination task and the polyprotein detection task in combination.

However, whether the testing task is one or a combination of multiple tasks, the whole blood sample needle 3 will always aspirate the same volume (30 microliters in this embodiment) of whole blood in step S1, and the whole blood sample needle 3 will always add the same volume (10 microliters in this embodiment) of whole blood to the hemolysis cup 21 in step S2, and then the subsequent testing task will be performed.

The working mode of the whole blood sample needle 3 is always unchanged, therefore, in any case, the whole blood sample needle 3 always sucks 30 microliters of whole blood sample and then sequentially discharges 10 microliters and 20 microliters of whole blood sample, so that the error of the whole blood sample needle 3 is fixed in the cases of an independent blood routine examination task, an independent single protein detection task, a blood routine examination task plus a single protein detection task, or a blood routine examination task plus a multiple protein detection task, and can be eliminated and weakened at a later stage, thereby relieving the problem of sample adding error caused by mixed use of multiple detection modes, and improving the overall detection accuracy of the whole blood cell classification and counting combined protein detector.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. A kind of blood cell differential count unites the whole blood protein detector, characterized by that, including:

a rack (1) provided with a sample pre-storage position (11) for placing a sample test tube, a whole blood sample adding position (12) for adding a whole blood sample into a container, a blood routine examination position (13) for performing blood routine examination on the whole blood sample, a reagent storage area (14) for placing a protein reagent, a transfer sample position (15) for adding the protein reagent into the container and a protein detection position (16) for performing protein detection;

a plurality of reaction cups (2), wherein the reaction cups (2) are divided into a blood dissolving cup (21) and a detection cup (22);

a rotating platform (5) which is arranged on the rack (1), is used for placing each reaction cup (2) and is used for driving each reaction cup (2) to move according to a preset circumferential carrying path, and the circumferential carrying path passes through the protein detection position (16), the whole blood sampling position (12) and the transfer sample position (15);

a whole blood sample needle (3) for loading the whole blood sample into the hemolyzing cup (21);

a whole blood carrying unit (6) which is arranged on the rack (1) and connected to the whole blood sample needle (3) and used for driving the whole blood sample needle (3) to move according to a preset whole blood carrying path, wherein the whole blood carrying path passes through the sample pre-storage position (11), the whole blood loading position (12) and the blood routine examination position (13), and the whole blood carrying path and the circular carrying path intersect at the whole blood loading position (12);

a transfer sample needle (4) for loading the sample in the hemolyzing cup (21) into the measuring cup (22) and adding the reagent in the reagent storage area (14) into the measuring cup (22);

a sample transfer unit (7) disposed on the rack (1) and connected to the transfer sample needle (4) for moving the transfer sample needle (4) according to a preset transfer carrying path, the transfer carrying path passes through the reagent storage area (14) and the transfer sample site (15), and the transfer carrying path intersects with the circumferential carrying path at the transfer sample site (15).

2. The apparatus according to claim 1, wherein the apparatus comprises: the test cup (22) is divided into a first test cup (221) and a second test cup (222), and the reagent storage area (14) includes a first protein reagent site corresponding to the first test cup (221) and a second protein reagent site corresponding to the second test cup (222).

3. The apparatus according to claim 2, wherein the apparatus comprises: the detection cups (22) are circumferentially distributed around the rotating platform (5), the blood dissolving cups (21) and the detection cups (22) are distributed in a staggered mode, and the first detection cups (221) and the second detection cups (222) are distributed in a staggered mode.

4. The apparatus according to claim 2, wherein the apparatus comprises: the reagent storage zone (14) comprises a diluting reagent site (145), the first protein reagent site, the second protein reagent site, and the transfer sample site (15) being spaced apart along an arcuate path.

5. The apparatus according to claim 1, wherein the apparatus comprises: the whole blood carrying path is distributed along a line tangential to the circumferential carrying path, and the whole blood loading site (12) is located at a tangential position.

6. A detection method implemented by the differential blood count apparatus according to claim 1 in combination with a whole blood protein detector, the detection method comprising:

aspirating a whole blood sample from the sample pre-storage site (11) through the whole blood carrying unit (6) and the whole blood sample needle (3);

loading the whole blood sample into the lysis cup (21) of the whole blood loading site (12) through the rotary platform (5), the whole blood carrying unit (6) and the whole blood sample needle (3);

and executing corresponding detection tasks based on the current detection items, wherein the detection tasks comprise a blood routine examination task and a protein detection task, and the protein detection task comprises a single protein detection task and a multiple protein detection task.

7. The method according to claim 6, wherein the step of executing the corresponding detection task based on the current detection item comprises:

-moving the whole blood sample needle (3) to the blood routine test site (13) by means of the whole blood carrier unit (6);

loading the whole blood sample in the lysis cup (21) through the rotary platform (5) and the transfer sample needle (4) into a detection cup (22) of the transfer sample site (15);

adding the protein reagent to the detection cup (22) of the transfer sample site (15);

and moving the detection cup (22) to the protein detection position (16) for protein detection.

8. The detection method according to claim 7, characterized in that: the detection cup (22) is divided into a first detection cup (221) and a second detection cup (222), and the first detection cup (221) and the second detection cup (222) are used for carrying out different protein detections on the same whole blood sample;

the step of loading the whole blood sample in the lysis cup (21) into the detection cup (22) of the transfer sample site (15) via the rotary platform (5) and the transfer sample needle (4) comprises:

-the rotary platform (5) moves the cuvette (21) to the transfer sample position (15), the transfer sample needle (4) aspirating a first designated volume of the whole blood sample in the cuvette (21);

-the rotary platform (5) moves the first detection cup (221) to the transfer sample position (15), the transfer sample needle (4) loading a first designated volume of the whole blood sample into the first detection cup (221);

-the rotating platform (5) moves the cuvette (21) to the transfer sample position (15), the transfer sample needle (4) aspirating a second designated volume of whole blood sample in the cuvette (21);

the rotary platform (5) moves the second test cup (222) to the transfer sample position (15), and the transfer sample needle (4) loads a second designated volume of whole blood sample into the second test cup (222).

9. The detection method according to claim 7, characterized in that: the detection cup (22) is divided into a first detection cup (221) and a second detection cup (222), and the first detection cup (221) and the second detection cup (222) are used for carrying out different protein detections on the same whole blood sample;

-the rotating platform (5) moves the cuvette (21) to the transfer sample position (15), the transfer sample needle (4) aspirating a third designated volume of whole blood sample in the cuvette (21);

-the rotary platform (5) moves the first detection cup (221) to the transfer sample position (15), the transfer sample needle (4) loading a first designated volume of whole blood sample into the first detection cup (221);

the rotating platform (5) moves the second test cup (222) to the transfer sample position (15), and the transfer sample needle (4) loads a second designated volume of whole blood sample into the second test cup (222).

10. The detection method according to claim 9, characterized in that: the third designated volume is greater than a sum of the first designated volume and the second designated volume.