CN114689831A - Blood analyzer and blood analysis method - Google Patents
Blood analyzer and blood analysis method Download PDFInfo
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- CN114689831A CN114689831A CN202011635443.XA CN202011635443A CN114689831A CN 114689831 A CN114689831 A CN 114689831A CN 202011635443 A CN202011635443 A CN 202011635443A CN 114689831 A CN114689831 A CN 114689831A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 51
- 239000008280 blood Substances 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims description 12
- 238000004159 blood analysis Methods 0.000 title claims description 8
- 238000005259 measurement Methods 0.000 claims abstract description 125
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 239000000523 sample Substances 0.000 claims description 183
- 239000003153 chemical reaction reagent Substances 0.000 claims description 45
- 239000012470 diluted sample Substances 0.000 claims description 18
- 238000011546 CRP measurement Methods 0.000 claims description 11
- 238000004140 cleaning Methods 0.000 claims description 8
- 210000005259 peripheral blood Anatomy 0.000 claims description 5
- 239000011886 peripheral blood Substances 0.000 claims description 5
- 238000001917 fluorescence detection Methods 0.000 claims description 2
- 239000000975 dye Substances 0.000 claims 1
- 238000012864 cross contamination Methods 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 4
- 239000003085 diluting agent Substances 0.000 abstract description 3
- 238000005070 sampling Methods 0.000 abstract description 3
- 102100032752 C-reactive protein Human genes 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000003219 hemolytic agent Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
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Abstract
The invention relates to the field of sample detection, and particularly discloses a blood analyzer and a blood separation method, which comprise a sample collection and distribution module, a liquid path support module, a measurement module and a control module, wherein the detection of WBC, DIFF, RBC, RET, CRP and SAA can be completed by one-time sampling through the mutual cooperation of the modules, wherein the detection samples of RBC and RET are derived from WBC sample diluent, the sample consumption is greatly reduced, the number of sample distribution branches is reduced, and the cross contamination can be prevented.
Description
Technical Field
The invention relates to the field of sample detection, in particular to a blood analyzer and a blood analysis method.
Background
Currently, the measurement of parameters such as RET, CRP, SAA and the like is increased while the conventional parameters of blood are measured, but the increase of the measurement parameters of the prior art is usually accompanied by the increase of blood consumption, for example, the increase of the RET parameter test requires that a RET channel is separately added, a blood sample is specially distributed to a RET reaction pool, and the sample consumption is increased.
In addition, measurement of multiple parameters requires distribution of blood samples to different reagents of different detection modules, and multi-stage distribution can reduce detection accuracy and cause contamination of blood samples. In order to reduce such contamination, the prior art usually adds a predetermined volume of sample to be discarded before the blood sample is distributed into another channel, and this method of adding a blood distribution step by adding a channel greatly increases the blood consumption in multi-parameter mode measurement, and this high sample consumption detection mode limits the test for the population with difficult blood collection.
Disclosure of Invention
Because an instrument for simultaneously detecting three parameters of RET, SAA and CRP does not exist at present, the invention provides a blood analyzer and a blood separation method, which aim to solve the problems of the requirement of the conventional combined detection of new three major blood (WBC, SAA and CRP), large blood consumption in multi-parameter sample detection and the like, and provide the blood analyzer which needs less blood and reduces the detection pollution of the conventional blood and the combined detection of RET, CRP and SAA.
In order to achieve the purpose, the invention adopts the following technical means:
a blood analyzer, comprising:
and the sample collection and distribution module is used for collecting samples and distributing the collected samples to each measurement module, and the samples are whole blood samples or peripheral blood samples.
And the liquid path support module is used for providing liquid path support for each measurement module and each sample acquisition and distribution module.
A plurality of measurement modules, each measurement module for measuring a dispensed sample to obtain a different parameter, the plurality of measurement modules including a WBC measurement module, a DIFF measurement module, a RET measurement module, a RBC measurement module, a SAA measurement module, and a C-reactive protein measurement module;
a control module: controlling a sample acquisition and distribution module to perform primary acquisition on a sample to be measured, and distributing the sample to each measurement module according to a preset sequence; and controlling the liquid path support module to dilute the sample distributed for the first measurement, and controlling the sample collection and distribution module to distribute the diluted part of the sample to the other measurement modules according to a preset sequence.
Preferably, the sample collection and distribution module comprises a sample injector, a sample needle and a sample needle movement control unit, wherein the sample injector is positioned between the sample needle and the plurality of measurement modules and used for providing a power source for collecting and distributing samples, the sample injector is pulled down to realize sample collection, and is pushed up to realize sample distribution; the sample needle movement control unit is used for controlling the sample needle to move in the horizontal direction or the vertical direction, so that the sample needle can be sampled and distributed at a specific position;
the liquid path support module comprises a reagent adding module and a transfusion pipeline, and the control system controls the reagent adding module to provide reagents for detection and cleaning to each measuring module through the transfusion pipeline, so that the sample collecting and distributing module is provided with a cleaning reagent.
Preferably, the RBC measurement module is an impedance counter and the RET measurement module is a fluorescence detection.
In another aspect of the present invention, there is provided a blood analysis method comprising
(a) The sample acquisition and distribution module is used for acquiring a sample to be measured at one time and distributing the sample to each measurement module according to a preset sequence;
(b) controlling a liquid path support module to dilute the samples distributed by the measurement modules;
(c) controlling the acquisition and distribution module to distribute the diluted part of samples to the rest of measurement modules according to a preset sequence;
(d) detecting parameter values of all measurement modules;
the sample to be detected is whole blood or peripheral blood.
Preferably, the blood analysis method includes:
(a) the sample acquisition and distribution module is used for acquiring a sample to be measured at one time and distributing the sample to the WBC measurement module, the DIFF measurement module, the SAA measurement module and the CRP measurement module according to a preset sequence;
(b) controlling the liquid path support module to dilute the sample distributed by the WBC measurement module;
(c) the control liquid path support module adds a reagent to the samples distributed by the IFF measurement module, the SAA measurement module and the CRP measurement module to prepare a sample liquid to be detected;
(d) controlling a sample collection and distribution module to carry out secondary collection on the diluted sample of the WBC measurement module, and distributing the diluted sample to an RET measurement module and an RBC measurement module according to a preset sequence;
(e) the control liquid path support module adds reagents to the rest diluted samples of the WBC measurement module and the diluted samples distributed by the RET measurement module and the RBC measurement module to prepare sample liquid to be detected;
(f) and measuring each sample liquid to be measured according to the program.
Preferably, the reagent added in the RET measurement module comprises a dye.
Preferably, the above steps (a) and (b) can be carried out simultaneously or sequentially, preferably (a) is carried out before (b).
1. Sequentially carrying out: (1) blood samples are first dispensed into designated measurement modules and the corresponding reagents are added. Before the blood sample is added, the sample pool of the measurement module is empty, and the sample needle is used for respectively adding the sample to each measurement module, so that the measurement module is not polluted by other reagents, and the waste of the blood sample caused by discarding a section of sample for avoiding pollution in the prior art is avoided; then through reagent adding module, reagent is added to mix with blood sample to play the role of dilution or detection preparation.
(2) The reagent is added firstly, then the sample is added, the sample needle needs to be inserted into the lower part of the liquid surface for better mixing of the sample and the reagent, and the sample needle needs to be cleaned after each sample addition.
2. The sample needle and the reagent are added simultaneously by the sample needle and the reagent adding module, so that the sample and the reagent can be quickly mixed, the sample adding time is shortened, but the reagent added by each measuring module is not completely the same, so that the sample needle can be polluted when the sample and the reagent are mixed, and in order to prevent each measuring module from being polluted, the sample needle needs to be cleaned after each adding.
The invention has the beneficial effects that:
1. a sample acquisition and distribution module of the sample analyzer samples a sample to be detected for one time, and then distributes the acquired sample to part of the measurement modules, but not all the modules, according to a preset sequence; when the whole blood sample is distributed to a part of the measuring modules in the first round, the diluted sample is sucked and distributed to the rest measuring modules after the whole blood sample is cleaned, sample distribution branches are reduced, cross contamination can be avoided, the blood consumption is not increased when detection parameters are increased, and the total sample amount is small.
2. The detection of the blood conventional cascade RET, CRP and SAA is realized.
3. The sample is added firstly, the reagent is added, the sample needle does not need to be cleaned, and a section of sample does not need to be discarded, so that the pollution of the sample to a subsequent sample can be avoided.
Drawings
FIG. 1 is a flow chart of a method of blood separation;
fig. 2 is a schematic diagram of a fluid path structure of the sample analyzer.
The reference numbers in the figures denote:
1-sample collection and distribution module, 101-sample syringe, 102-sample needle, 103-sample needle movement control unit, 2-reagent addition module, 3-measurement module, 301-WBC measurement module, 302-DIFF measurement module, 303-SAA measurement module, 304-CRP measurement module, 305-RBC measurement module, and 306-RET measurement module
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The invention provides a blood analyzer and a blood separation method, which aim to solve the requirements of conventional (WBC, SAA and CRP) joint detection of three new blood samples and the problems of large blood consumption, cross contamination and the like in multi-parameter sample detection.
As shown in fig. 1 and 2, a blood analyzer includes:
the sample collection and distribution module 1 is used for collecting samples, and distributing the collected samples to various measurement modules, wherein the samples are whole blood samples or peripheral blood samples.
The sample collection and distribution module 1 comprises a sample injector 101, a sample needle 102 and a sample needle movement control unit 103, wherein the sample injector 101 is positioned between the sample needle 102 and the plurality of measurement modules and used for providing a power source for collecting and distributing samples, the sample injector 101 is pulled down to realize sample collection, and is pushed up to realize sample distribution; the sample needle movement control unit 103 is used for controlling the sample needle 102 to move in the horizontal direction or the vertical direction, so as to realize the sampling and distribution of the sample needle 102 at a specific position;
the liquid path support module comprises a reagent adding module 2 and a transfusion pipeline, wherein the control system controls the reagent adding module 2 to provide reagents for detection and cleaning for each measuring module through the transfusion pipeline, provides cleaning reagents for the sample collecting and distributing module 1, and is used for providing liquid path support for each measuring module and the sample collecting and distributing module.
A plurality of measurement modules 3, each for measuring a dispensed sample to obtain a different parameter, the plurality of measurement modules including a WBC measurement module 301, a DIFF measurement module 302, a SAA measurement module 303, a CRP measurement module 304, a RBC measurement module 305, and a RET measurement module 306;
the measuring module 3 provides a necessary place for the mixed reaction of the sample and the reagent, the sample liquid to be detected is stored, and after the sample and the reagent are added into the measuring module 3, the sample and the reagent react in the measuring module 3 to obtain the sample liquid to be detected.
A control module: controlling a sample acquisition and distribution module to perform primary acquisition on a sample to be measured, and distributing the sample to each measurement module according to a preset sequence; and controlling the liquid path support module to dilute the sample distributed for the first measurement, and controlling the sample collection and distribution module to distribute the diluted part of the sample to the other measurement modules according to a preset sequence.
The RBC measurement module is used for counting impedance, and the RET measurement module is used for detecting fluorescence.
In the embodiment of the present invention, the sample needle movement control unit 103 controls the sample needle 102 to move to the sample collection position, and the sample injector 101 is pulled down for a certain stroke to complete quantitative sample collection. The sample needle movement control unit 103 controls the sample needle 102 to move to the WBC measurement module 301 sample dispensing position, the sample injector 101 pushes up the dispensing portion to quantify the blood sample, and the reagent adding module 2 dispenses the reagent to the WBC measurement module 301 at the same time, so as to realize the preliminary mixing and dilution of the sample; in the same way, different quantitative whole blood samples of whole blood are sequentially distributed into the DIFF measurement module 302, the SAA measurement module 303 and the CRP measurement module 304 through the sample collection and distribution module 1, and meanwhile, a reagent adding module 2 distributes quantitative reagents of specific types into the DIFF measurement module 302, the SAA measurement module 303 and the CRP measurement module 304. And fully and uniformly mixing the obtained mixture in respective reaction containers, and reacting to complete preparation of sample liquid to be detected of DIFF, SAA and CRP.
After all the whole blood sample is dispensed, reagent addition module 2 provides a cleaning reagent to sample collection and dispensing module 1 to complete the cleaning of sample needle 102. After the sample in the WBC measurement module 301 is fully diluted, the sample needle movement control unit 103 controls the sample needle 102 to move to the WBC measurement module 301, the sample injector 101 pulls down for a certain stroke to suck a certain amount of diluted sample liquid in the WBC measurement module 301, then the sample needle movement control unit 103 controls the sample needle 102 to move to the sample distribution position of the RET measurement module 305, the sample injector 101 of the sample collection and distribution module 1 pushes up for a certain stroke to distribute a certain amount of diluted sample liquid into the RET measurement module 305, and the reagent addition module 2 distributes a certain amount of reaction reagent and dye into the RET measurement module 305, and the RET sample liquid to be measured for RET detection is prepared after fully mixing. Similarly, after the sample collection and distribution module 1 distributes the sample into the RET measurement module 305, the sample needle movement control unit 103 controls the sample needle 102 to move to the RBC measurement module 306 at the sample distribution position, a certain amount of diluted sample liquid is distributed into the RBC measurement module 306 through the sample injector 101 of the sample collection and distribution module 1, and a certain amount of diluent liquid is added into the RBC measurement module 306 through the reagent adding module 2, and the diluent liquid and the distributed diluted sample liquid are fully mixed, so that the preparation of the RBC sample liquid to be tested for the RBC impedance counting is completed. The total volume of diluted sample fluid dispensed in the RET measurement module 305 and the RBC measurement module 306 is less than the volume of diluted sample fluid drawn by the sample acquisition and dispensing module 1 from within the WBC measurement module 301. After the sample distribution module 1 sucks the diluted sample solution from the WBC measurement module 301, the reagent adding module 2 adds a quantitative hemolytic agent into the WBC measurement module 301, and the rest diluted sample solution in the WBC measurement module 301 and the newly added hemolytic agent are fully mixed to complete preparation of the WBC sample solution to be tested.
And the control system controls the instrument to complete the measurement of the sample liquid to be measured.
A sample collection and distribution module 1 of the sample analyzer performs primary sampling on a sample to be detected, and then distributes the collected sample to a WBC measurement module 301, a DIFF measurement module 302, an SAA measurement module 303 and a CRP measurement module 304 instead of all the modules according to a preset sequence; when the first round of whole blood sample distribution to a part of the measurement modules is completed, and the diluted sample is sucked and distributed to the RBC measurement module 305 and the RET measurement module 306 after the whole blood sample is cleaned, sample distribution branches are reduced, cross contamination can be avoided, the blood consumption is not increased when detection parameters are increased, the total sample amount is small, and the detection of the blood conventional cascade RET, CRP and SAA is realized.
Claims (8)
1. A blood analyzer, comprising:
and the sample collection and distribution module is used for collecting samples and distributing the collected samples to each measurement module, and the samples are whole blood samples or peripheral blood samples.
And the liquid path support module is used for providing liquid path support for each measurement module and each sample acquisition and distribution module.
A plurality of measurement modules, each measurement module for measuring a dispensed sample to obtain a different parameter, the plurality of measurement modules including a WBC measurement module, a DIFF measurement module, a RET measurement module, a RBC measurement module, a SAA measurement module, and a C-reactive protein measurement module;
a control module: controlling a sample acquisition and distribution module to perform primary acquisition on a sample to be measured, and distributing the sample to each measurement module according to a preset sequence; and controlling the liquid path support module to dilute the sample distributed for the first measurement, and controlling the sample collection and distribution module to distribute the diluted part of the sample to the other measurement modules according to a preset sequence.
2. The blood analyzer of claim 1, wherein the sample collection and distribution module comprises a sample syringe, a sample needle, and a sample needle movement control unit, the sample syringe being located between the sample needle and the plurality of measurement modules for providing a power source for collection and distribution of the sample;
the sample needle movement control unit is used for controlling the sample needle to move in the horizontal direction or the vertical direction, so that the sample needle can be sampled and distributed at a specific position;
the liquid path support module comprises a reagent adding module and a transfusion pipeline, and the control system controls the reagent adding module to provide reagents for detection and cleaning to each measuring module through the transfusion pipeline, so that the sample collecting and distributing module is provided with a cleaning reagent.
3. The hematology analyzer of claim 1 or 2, wherein the RBC measurement module is an impedance count and the RET measurement module is a fluorescence detection.
4. A method of blood analysis, comprising:
(a) the sample acquisition and distribution module is used for acquiring a sample to be measured at one time and distributing the sample to each measurement module according to a preset sequence;
(b) controlling a liquid path support module to dilute the samples distributed by the measurement modules;
(c) controlling the acquisition and distribution module to distribute the diluted part of samples to the rest of measurement modules according to a preset sequence;
(d) detecting parameter values of all measurement modules;
the sample to be detected is whole blood or peripheral blood;
5. the blood analysis method according to claim 4, comprising:
(a) the sample acquisition and distribution module is used for acquiring a sample to be measured at one time and distributing the sample to the WBC measurement module, the DIFF measurement module, the SAA measurement module and the CRP measurement module according to a preset sequence;
(b) controlling the liquid path support module to dilute the sample distributed by the WBC measurement module;
(c) the control liquid path support module adds a reagent to the samples distributed by the IFF measurement module, the SAA measurement module and the CRP measurement module to prepare a sample liquid to be detected;
(d) controlling a sample collection and distribution module to carry out secondary collection on the diluted sample of the WBC measurement module, and distributing the diluted sample to an RET measurement module and an RBC measurement module according to a preset sequence;
(e) the control liquid path support module adds reagents to the rest diluted samples of the WBC measurement module and the diluted samples distributed by the RET measurement module and the RBC measurement module to prepare a sample liquid to be detected;
(f) and measuring each sample liquid to be measured according to the program.
6. The method of claim 5, wherein the reagents added to the RET measurement module comprise dyes.
7. The blood analysis method of any one of claims 4-6, wherein steps (a) and (b) are not limited by order.
8. The method of claim 7, wherein step (a) is preceded by step (b).
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Cited By (1)
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CN115166259A (en) * | 2022-07-12 | 2022-10-11 | 深圳赛斯鹏芯生物技术有限公司 | Whole blood protein detector combining blood cell classification and counting and detection method thereof |
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CN107656068A (en) * | 2014-07-01 | 2018-02-02 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of blood detector |
CN109932520A (en) * | 2019-04-19 | 2019-06-25 | 深圳市理邦精密仪器股份有限公司 | Blood test device |
CN112129931A (en) * | 2019-06-24 | 2020-12-25 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection equipment and measurement mode selection method |
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CN107656068A (en) * | 2014-07-01 | 2018-02-02 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of blood detector |
CN104713816A (en) * | 2015-02-04 | 2015-06-17 | 深圳市帝迈生物技术有限公司 | Whole blood CRP detection apparatus, method thereof, and blood cell analyzer |
CN109932520A (en) * | 2019-04-19 | 2019-06-25 | 深圳市理邦精密仪器股份有限公司 | Blood test device |
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