CN219201334U - Flow type fluorescence detection liquid path system - Google Patents

Abstract

The utility model discloses a flow type fluorescence detection liquid path system, which aims to solve the problems of low detection efficiency and low device utilization rate in the prior art, and comprises a flow chamber, a sheath liquid supply module, a sampling device, a first quantitative module, a second quantitative module, a first valve group and a second valve group, wherein the sheath liquid supply module is connected with a sheath liquid inlet, the first quantitative module and the second quantitative module through the first valve group, the first quantitative module is connected with a sample inlet and the sampling device through the second valve group, and the second quantitative module is connected with the sample inlet and the sampling device through the second valve group.

Description

Flow type fluorescence detection liquid path system

Technical Field

The utility model belongs to biological detection technology, in particular to a flow type fluorescence detection liquid path system.

Background

Flow fluorescent detection, also known as suspension array, liquid phase chip, etc. The principle of the technology is that a fluorescent coding microsphere is taken as a core, a microneedle molecule on the fluorescent coding microsphere can be specifically combined with a biomolecule to achieve the effect of fluorescent labeling of the biomolecule, laser analysis is carried out on the labeled biomolecule, and a series of treatment is carried out on an optical signal generated by the laser analysis to obtain a visual diagnosis result.

The utility model of China of the prior publication number CN215574651U discloses a flow type fluorescence detection liquid path system, which utilizes the fluorescence detection technology, and comprises a flow chamber, a sampling device, a cleaning device, a sheath liquid pump and a sample pump, adopts various valves to control the flow regulation of media (including samples and sheath liquid) in a pipeline, and realizes the flow of sample suction, sample pushing, detection and cleaning.

However, the existing liquid path system is not perfect enough and has the following problems: (1) In the process of detecting a sample by the liquid path system, the sampling device, the cleaning device, the sheath pump and the flow chamber are in empty states to different degrees respectively, so that the problem of low utilization rate of components in the liquid path system is caused; (2) When a plurality of samples are continuously detected, the liquid path system can only detect the plurality of samples one by one, and only after the last sample is detected, the liquid path system can start the detection of the next sample, which leads to the problem of low detection efficiency.

Disclosure of Invention

In order to overcome the defects and the existing problems in the prior art, the utility model provides a flow type fluorescence detection liquid path system.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a flow-type fluorescence detection fluid path system, comprising:

a flow cell comprising a sample inlet, a sheath fluid inlet, and a waste fluid outlet;

a sheath fluid supply module for supplying sheath fluid to the sheath fluid inlet, the first dosing module, and the second dosing module;

a sampling device for providing a sample to the first and second quantification modules;

the first quantitative module is used for sucking and cleaning the sheath liquid of the first quantitative module and the sampling device through the sheath liquid supply module and is also used for quantitatively sucking a sample through the sampling device;

the second quantifying module is used for sucking and cleaning the sheath liquid of the second quantifying module and the sampling device through the sheath liquid supply module and is also used for quantitatively sucking the sample through the sampling device;

the sheath liquid supply module is connected to the sheath liquid inlet, the first quantitative module and the second quantitative module through the first valve group;

and the second quantitative module is connected to the sample inlet and the sampling device through the second valve group.

Preferably, the first valve group includes a third shut-off valve, and the sheath fluid supply module is connected to the first metering module through the third shut-off valve.

Preferably, the first valve group includes a fourth shut-off valve, and the sheath fluid supply module is connected to the second dosing module through the fourth shut-off valve.

Preferably, the first valve group includes a fifth shut-off valve, and the sheath fluid inlet is connected to the sheath fluid supply module through the fifth shut-off valve.

Preferably, the second valve group comprises a first multi-way valve, and the first metering module is connected to the sample inlet and the sampling device through the first multi-way valve.

Preferably, the second valve group comprises a second multi-way valve, and the second quantifying module is connected to the sample inlet and the sampling device through the second multi-way valve.

Preferably, the first multi-way valve and the second multi-way valve are three-way valves.

Preferably, the first quantitative module comprises two first flow passage openings, the first valve group and the second valve group are respectively connected to the two first flow passage openings, the second quantitative module comprises two second flow passage openings, and the first valve group and the second valve group are respectively connected to the two second flow passage openings.

Preferably, the waste liquid treatment apparatus further comprises a waste liquid collector and a sixth shut-off valve, wherein the waste liquid collector is connected to the waste liquid outlet through the sixth shut-off valve.

On the other hand, the utility model also provides a detection method adopting the flow type fluorescence detection liquid path system, which comprises the following steps:

detection procedure using the first quantitative module: the first quantitative module quantitatively absorbs the sample through the sampling device, the first quantitative module pushes the sample to the flow chamber, the fluorescence detector detects the concentration of the analyte in the sample from the first quantitative module on the flow chamber, and the first quantitative module absorbs the sheath liquid through the sheath liquid providing module and cleans the first quantitative module and the sampling device through the sheath liquid;

detection procedure using the second quantification module: the second quantitative module quantitatively absorbs the sample through the sampling device, the second quantitative module pushes the sample to the flow chamber, the fluorescence detector detects the concentration of the analyte in the sample from the second quantitative module on the flow chamber, and the second quantitative module absorbs the sheath liquid through the sheath liquid providing module and cleans the second quantitative module and the sampling device through the sheath liquid;

the detection process using the first dosing module and the detection process using the second dosing module are performed simultaneously, and if the first dosing module pushes the sample to the flow chamber, the second dosing module sucks sheath fluid through the sheath fluid supply module and washes the first dosing module and the sampling device through the sheath fluid.

Preferably, the method further comprises:

if the second quantitative module pushes the sample to the flow chamber, the first quantitative module absorbs the sheath fluid through the sheath fluid providing module and washes the first quantitative module and the sampling device through the sheath fluid.

Preferably, the method further comprises:

if the fluorescence detector detects the concentration of the analyte in the sample from the first dosing module on the flow chamber, the second dosing module draws sheath fluid through the sheath fluid supply module and washes the first dosing module and the sampling device through the sheath fluid.

Preferably, the method further comprises:

if the fluorescence detector detects the concentration of the analyte in the sample from the second quantification module on the flow chamber, the first quantification module draws sheath fluid through the sheath fluid supply module and washes the first quantification module and the sampling device through the sheath fluid.

Preferably, the method further comprises:

if the fluorescence detector detects the concentration of the analyte in the sample from the first dosing module on the flow chamber, the first dosing module draws sheath fluid through the sheath fluid supply module and washes the first dosing module and the sampling device through the sheath fluid.

Preferably, the method further comprises:

if the fluorescence detector detects the concentration of the analyte in the sample from the second quantification module on the flow chamber, the second quantification module draws sheath fluid through the sheath fluid supply module and washes the second quantification module and the sampling device through the sheath fluid.

Compared with the prior art, the utility model has the following outstanding and beneficial technical effects:

(1) In the utility model, the first quantitative module and the second quantitative module share the sampling device, the flow chamber and the sheath liquid supply module, so that the use efficiency of each device in the liquid path system is greatly improved, and the structure of the liquid path system is simplified under the requirement of ensuring sampling, pushing, measuring and cleaning.

(2) In the utility model, the first quantitative module and the second quantitative module alternately use the sampling device, the flow chamber and the sheath liquid supply device, on one hand, the first quantitative module and the second quantitative module are respectively and independently sampled, corresponding devices are cleaned before sampling, so that the problem of mutual pollution of samples in a detection flow using the first quantitative module and samples in a detection flow using the second quantitative module is avoided, on the other hand, the first quantitative module can be cleaned and sampled during sample pushing and sample measuring, for example, the second quantitative module can be cleaned during sample pushing to the flow chamber by the first quantitative module, the second quantitative module and the sampling device can be cleaned by the second quantitative module, the use rate of each device in a liquid path system is greatly improved, and in practical use, the liquid path system shows higher efficiency during detection of a plurality of groups of samples (such as repeated experiments and control samples).

(3) In the utility model, the control of the flow and the direction of the medium (comprising the sample and the sheath liquid) in the liquid path system is realized by controlling the first valve group and the second valve group, so that the working reliability of the liquid path system is ensured.

Drawings

FIG. 1 is a schematic diagram of a fluid circuit system according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram of a fluid circuit system according to another embodiment of the present utility model;

in the figure: the device comprises a 1-waste liquid collecting module, a 2-sampling device, a 3-sheath liquid providing module, a 4-flow chamber, a 5-first quantitative module, a 6-second quantitative module, a 7-first multi-way valve, an 8-second multi-way valve, a 9-third shut-off valve, a 10-fourth shut-off valve, a 11-fifth shut-off valve, a 12-sixth shut-off valve, a 13-seventh multi-way valve, a 14-eighth shut-off valve, a 15-ninth shut-off valve, a 16-tenth shut-off valve, a 17-eleventh shut-off valve, a 21-sampling needle, a 22-sampling port, a 41-sample inlet, a 42-sheath liquid inlet, a 43-waste liquid outlet, a 51-first flow channel port and a 61-second flow channel port.

Detailed Description

The present utility model is further described below with reference to the drawings and specific embodiments for the purpose of facilitating understanding by those skilled in the art.

As shown in fig. 1 and 2, the present utility model provides a flow type fluorescence detection liquid path system for detecting a liquid phase sample, which includes a flow chamber, a sheath liquid supply module, a sampling device, a first metering module, a second metering module, a first valve group, a second valve group, a waste liquid collection module, and a sixth shut-off valve. The devices of the liquid path system are connected together by pipelines to form the liquid path system. The sampling device has a sampling port through which the sample enters the fluid path system.

The first quantitative module and the second quantitative module share the same sampling device, the same sheath liquid providing module and the same flow chamber, and in use, the sampling device, the sheath liquid providing module and the flow chamber can be alternately used by the first quantitative module and the second quantitative module, so that the problem that the sampling device, the sheath liquid providing module and the flow chamber are empty is avoided, and the utilization rate of the sampling device is greatly improved.

Before use, a sample is arranged on a sampling device with a sampling port, and sheath liquid is arranged on a sheath liquid supply module.

The use of the present fluid path system includes the use of a first dosing module and the use of a second dosing module. When the first quantitative module is used, the first quantitative module is controlled to sequentially absorb and push samples, the fluorescent detector measures optical signals of the samples on the flow chamber, the concentration of the analyte in the samples in the liquid path system is calculated according to the fluorescent intensity of the samples and the pushed samples, the purpose of quantitative detection is achieved, and finally the first quantitative module is controlled to absorb sheath liquid to clean the first quantitative module and the sampling device. Similarly, when the second quantitative module is used, the second quantitative module is controlled to sequentially absorb and push samples, the fluorescent detector measures optical signals of the samples on the flow chamber, and the concentration of the analyte in the samples in the liquid path system is calculated according to the optical signal intensity of the samples and the push samples.

For ease of understanding, the present embodiment explains the technical terms described above.

Sucking samples: the first/second dosing module absorbs the sample by the sampling device.

Pushing: the first/second metering module pushes the sample in the first/second metering module into the flow chamber.

And (3) sample measurement: the fluorescence detector optically detects the sheath fluid-sample flow through the flow cell to determine the concentration of the analyte in the sample.

Sample suction amount: the first quantitative module/the second quantitative module sucks the sample amount in the first quantitative module/the second quantitative module when sucking the sample.

Sample pushing amount: the first/second metering module pushes the sample amount into the flow chamber at the time of sample pushing.

Sample of

The sample is in the liquid phase and includes the analyte and the fluorescent encoded spheres.

Any analyte present in a sample that is capable of undergoing a specific reaction can be detected using this embodiment. Some analytes can be detected using the present fluid pathway system, and for example, analytes can include proteins, polypeptides, enzymes, hormones, antibodies, target nucleic acid fragments, and the like, which are biologically active substances capable of specifically reacting with fluorescent-encoded microspheres, such analytes being widely present in whole blood, serum, plasma, urine, saliva.

The fluorescent coded microsphere is a microsphere with fluorescent markers and molecular probes, which is spherical in shape and has a particle size of usually between 1 nanometer and 500 micrometers, and in this embodiment, the particle size is preferably 5.5 to 5.6 micrometers. The surface or interior of the microsphere is loaded with fluorescent markers, also known as fluorescent molecules, which can trigger a stronger optical signal upon weaker laser stimulation. For example, fluorescent labels may include molecular phycoerythrin, fluorescein isothiocyanate, 7-amino-4-methylcoumarin, and the like. The molecular probes are capable of specifically binding to the analyte such that the fluorescent-encoded microspheres are labeled on the analyte, and the labeled analyte can be captured in the flow cell by the fluorescent detector as an image of the optical signal.

Flow cell

The flow cell belongs to a core device in a liquid path system, and the quality of the flow cell directly influences the fluorescence detection result of the fluorescence detector on the marked analyte. The flow cell is made of a transparent and chemically stable material such that the laser light emitted by the laser emitter and the optical signal reflected by the labeled analyte can pass through the flow cell. The transparent and chemically stable material is typically glass. The flow chamber comprises a sample inlet through which the sample of the first metering module or the sample provided by the second metering module may enter the conduit inside the flow chamber. The axis of the sample inlet is located on the axis of the flow chamber interior conduit such that sample entering the flow chamber interior conduit from the sample inlet can flow along the axis of the flow chamber interior conduit. The flow chamber further comprises a sheath fluid inlet through which sheath fluid provided by the sheath fluid providing module may enter the conduit inside the flow chamber. The sheath fluid inlet is located on a side of the flow chamber interior conduit such that sheath fluid entering the flow chamber interior conduit from the sheath fluid inlet, the sheath fluid entraps the sample from around the sample, and the sheath fluid forces the sample to remain flowing on the axis of the flow chamber interior conduit. The flow chamber further comprises a waste liquid outlet for discharging waste liquid in the inner pipeline of the flow chamber, wherein the waste liquid refers to sheath liquid-sample flow detected by the laser detector. In addition to the above-described flow cell structure, other specific structures of the flow cell can be clearly and completely known to those skilled in the art of biological detection by well-known technical contents.

Sheath fluid supply module

The sheath liquid supply module of the liquid path system is provided with sheath liquid, and the sheath liquid supply module can supply sheath liquid to the flow chamber, the first quantitative module and the second quantitative module. In use, sheath fluid flowing from the sheath fluid module to the flow chamber is used to hold a sample, sheath fluid flowing from the sheath fluid module to the first dosing module is used to clean the first dosing module and the sampling device, and sheath fluid flowing from the sheath fluid module to the second dosing module is used to clean the second dosing module and the sampling device. In a specific embodiment, the sheath fluid supply module comprises a cavity, the cavity is used for storing sheath fluid, and the flow chamber, the first quantitative module and the second quantitative module are respectively connected with the cavity. In a specific embodiment, the sheath fluid supply module comprises a sheath fluid pump for delivering sheath fluid to facilitate delivery of the sheath fluid to the flow chamber, the first dosing module and the second dosing module.

Sampling device

The sampling device is especially a sampling device for biological detection. A sample may be provided on a sampling device that may collect a sample and provide the collected sample to a first quantification module and a second quantification module. Since the sample is in the liquid phase, the sample is typically placed in a tube, beaker, or the like container into which the sampling device is placed so that the sampling device draws the sample. In one embodiment, the sampling device comprises a sampling needle having a unitary needle-like structure, the opening of the container is typically sealed with a septum, and the sampling needle is adapted to pierce the septum and be inserted into the container so as to aspirate the sample from the container. In order to facilitate the penetration of the septum by the sampling needle, one end of the sampling needle may be sharpened and the sampling port disposed on the sharpened end of the sampling needle.

First metering module

The first quantitative module is used for quantitatively sucking or pushing a medium, and the medium can be a sample or sheath fluid.

In a specific embodiment, the first metering module comprises a metering pump, the metering pump refers to a hydraulic pump with the constant volume of medium discharged by one rotation of a pump shaft, the specific structure of the metering pump can be known through the prior art, the volume of the medium sucked or pushed by the first metering module can be calculated by counting the rotation number of the pump shaft of the metering pump, and in a specific embodiment, the first metering module further comprises a containing cavity which can be used for temporarily storing the medium, so that when the sampling device, the sheath fluid supply module and the flow chamber are alternately used by the first metering module and the second metering module, the first metering module and the sheath fluid supply module are mutually independent and complementary and do not interfere with each other. In one embodiment, the first metering module comprises two first fluid ports, the first valve group being connected to one first fluid port and the second valve group being connected to the other first fluid port, such that the control of the first valve group and the second valve group is independent of each other and does not interfere with each other.

In another embodiment, the first metering module comprises a chamber, the volume of which is known, and in use, the first metering module may fill the chamber with medium or drain medium from the chamber, as well as to achieve the effect of the empire metering or pushing the medium. In another embodiment, the first metering module further comprises a hydraulic pump for delivering the medium so that the first metering module sucks or pushes the medium.

Second quantitative module

The function and specific structure of the second quantitative module are consistent with those of the first quantitative module, the second quantitative module comprises two second flow passage openings, and the first valve group and the second valve group are respectively connected to the two second flow passage openings. The difference is that in use, the first dosing module and the second dosing module are alternating sampling devices, sheath fluid providing modules and flow chambers. For example, when the first quantitative module sucks a sample by the sampling device, the second quantitative module sucks sheath fluid by the sheath fluid supply module.

First valve group

The first valve group is used as a control device between the sheath liquid supply module and the sheath liquid inlet, and between the sheath liquid supply module and the first quantitative module, and between the sheath liquid supply module and the second quantitative module. In this embodiment, the sheath fluid supply module is connected to the sheath fluid inlet through a pipeline, and the first valve group is disposed on the pipeline between the sheath fluid supply module and the sheath fluid inlet, and is used for opening and closing the pipeline between the sheath fluid supply module and the sheath fluid inlet; the sheath fluid supply module is also connected with the first metering module through a pipeline, and the first valve group is also arranged on the pipeline between the sheath fluid supply module and the first metering module and is used for opening and closing the pipeline between the sheath fluid supply module and the first metering module; the sheath liquid providing module is connected with the second quantifying module through a pipeline, and the first valve group is further arranged on the pipeline between the sheath liquid providing module and the second quantifying module and used for opening and closing the pipeline between the sheath liquid providing module and the second quantifying module.

In a specific embodiment, as shown in fig. 1, the first valve group includes a third shut-off valve, the third shut-off valve is disposed on a pipeline between the sheath fluid supply module and the sheath fluid inlet, the sheath fluid supply module is connected to the sheath fluid chamber through the third shut-off valve, and the on-off between the sheath fluid supply module and the sheath fluid chamber can be independently controlled by controlling the third shut-off valve. In a specific embodiment, the first valve group further comprises a fourth shut-off valve, the fourth shut-off valve is arranged on a pipeline between the sheath fluid supply module and the sheath fluid inlet, the sheath fluid supply module is connected to the first metering module through the fourth shut-off valve, and the on-off between the sheath fluid supply module and the first metering module can be independently controlled through controlling the fourth shut-off valve. In a specific embodiment, the first valve group further comprises a fifth shut-off valve, the fifth shut-off valve is arranged on a pipeline between the sheath fluid supply module and the second quantifying module, the sheath fluid supply module is connected to the second quantifying module through the fifth shut-off valve, and the on-off between the sheath fluid supply module and the second quantifying module can be independently controlled through controlling the fifth shut-off valve.

In another specific embodiment, as shown in fig. 2, the first valve group includes a seventh multiple-way valve, the seventh multiple-way valve is disposed on a pipeline between the sheath fluid supply module and the first dosing module, the sheath fluid supply module is connected to the first dosing module through the seventh multiple-way valve, the seventh multiple-way valve is further disposed on a pipeline between the sheath fluid supply module and the second dosing module, the sheath fluid supply module is further connected to the second dosing module through the seventh multiple-way valve, and the on-off between the sheath fluid supply module and the second dosing module can be controlled through the seventh multiple-way valve, so that the structure of the first valve group is simplified. The multi-way valve refers to a valve with a plurality of passage openings, the seventh multi-way valve can be a three-way valve and is provided with three passage openings, the sheath liquid supply module, the first quantitative module and the second quantitative module are respectively connected to the three passage openings of the seventh multi-way valve, and in use, if the seventh multi-way valve is connected with the sheath liquid supply module and the first quantitative module, the seventh multi-way valve disconnects the sheath liquid supply module and the second quantitative module; if the seventh multi-way valve is used for switching on the sheath fluid supply module and the second quantitative module, the seventh multi-way valve is used for switching off the sheath fluid supply module and the first quantitative module, so that the first quantitative module and the second quantitative module can be alternately used by controlling the seventh multi-way valve.

Second valve group

The second valve group is used as a control device between the sampling device and the first quantitative module, between the sampling device and the second quantitative module, between the first quantitative module and the flow chamber, and between the second quantitative module and the flow chamber. In this embodiment, the sampling device is connected to the first metering module through a pipeline, and the second valve group is disposed on the pipeline between the sampling device and the first metering module, and is used for opening and closing the pipeline between the sampling device and the first metering module; the sampling device is also connected with the second quantifying module through a pipeline, and the second valve group is also arranged on the pipeline between the sampling device and the second quantifying module and is used for opening and closing the pipeline between the sampling device and the second quantifying module; the first metering module is also connected with the flow chamber through a pipeline, and the second valve group is also arranged on the pipeline between the first metering module and the flow chamber and used for opening and closing the pipeline between the first metering module and the flow chamber; the second quantitative module is also connected with the flow chamber through a pipeline, and the second valve group is also arranged on the pipeline between the second quantitative module and the flow chamber and is used for opening and closing the pipeline between the second quantitative module and the flow chamber.

As shown in fig. 1, in a specific embodiment, the first valve group includes a first multiple-way valve, the first multiple-way valve is disposed on a pipeline between the sampling device and the first metering module, the sampling device is connected to the first metering module through the first multiple-way valve, the first multiple-way valve is further disposed on a pipeline between the first metering module and the flow chamber, the first metering module is connected to the flow chamber through the first multiple-way valve, and the on-off between the sampling device and the first metering module and the on-off between the first metering module and the flow chamber can be controlled by controlling the first multiple-way valve, so that the structure of the first valve group is simplified. The first multi-way valve may be a three-way valve having three passage ports, the sampling device, the first metering module and the flow chamber being connected to the three passage ports of the first multi-way valve respectively, in use, if the first multi-way valve is to turn on the sampling device and the first metering module, the first multi-way valve disconnects the first metering module and the flow chamber; if the first multi-way valve is used for switching on the first quantitative module and the flow chamber, the first multi-way valve is used for switching off the sampling device and the first quantitative module, so that the sampling device and the flow chamber can be alternately used by controlling the first multi-way valve. In a specific embodiment, the second valve group further comprises a second multi-way valve, the second multi-way valve is arranged on a pipeline between the sampling device and the second quantifying module, the sampling device is connected to the second quantifying module through the second multi-way valve, the second multi-way valve is further arranged on a pipeline between the second quantifying module and the flowing chamber, the second quantifying module is connected to the flowing chamber through the second multi-way valve, and the on-off between the sampling device and the second quantifying module and the on-off between the second quantifying module and the flowing chamber can be controlled through controlling the second multi-way valve, so that the structure of the first valve group is simplified. The second multi-way valve may be a three-way valve having three passage ports, the sampling device, the second dosing module and the flow chamber being connected to the three passage ports of the second multi-way valve, respectively, in use, if the second multi-way valve is to turn on the sampling device and the second dosing module, the second multi-way valve disconnects the second dosing module and the flow chamber; and if the second multi-way valve is connected with the second quantitative module and the flow chamber, the second multi-way valve disconnects the sampling device and the second quantitative module, so that the sampling device and the flow chamber can alternately use the second quantitative module by controlling the second multi-way valve. In addition, through controlling first multiple unit valve and second multiple unit valve, still can reach the effect that first ration module and second ration module used sampling device, first ration module and second ration module used the flow chamber alternately.

In another embodiment, as shown in fig. 2, the first valve group includes an eighth shut-off valve, the eighth shut-off valve is disposed on a pipeline between the sampling device and the first metering module, the sampling device is connected to the first metering module through the eighth shut-off valve, and the on-off between the sampling device and the first metering module can be independently controlled by controlling the eighth shut-off valve. In another specific embodiment, the second valve group comprises a ninth shut-off valve, the ninth shut-off valve is arranged on a pipeline between the sampling device and the second quantifying module, the sampling device is connected to the second quantifying module through the ninth shut-off valve, and the on-off between the sampling device and the second quantifying module can be independently controlled through controlling the ninth shut-off valve. In another embodiment, the first valve group comprises a tenth shut-off valve, the tenth shut-off valve is arranged on a pipeline between the first metering module and the flow chamber, the first metering module is connected to the flow chamber through the tenth shut-off valve, and the on-off between the first metering module and the flow chamber can be independently controlled by controlling the tenth shut-off valve. In another embodiment, the second valve group further comprises an eleventh shut-off valve, the eleventh shut-off valve is arranged on a pipeline between the second quantifying module and the flow chamber, the second quantifying module is connected to the flow chamber through the eleventh shut-off valve, and the on-off between the second quantifying module and the flow chamber can be independently controlled through controlling the eleventh shut-off valve.

Waste liquid collecting module

The waste collection module is in particular a container for collecting waste of the biological type. The waste liquid refers to the sheath liquid-sample flow after detection. By collecting the waste liquid in the waste liquid collecting module, personal injury, environmental pollution and social hazard caused by the waste liquid with potential pathogenicity and harmfulness are avoided.

Sixth shut-off valve

The sixth shut-off valve acts as a control device between the waste outlet and the waste collection module. The sixth shut-off valve is arranged on a pipeline between the waste liquid outlet and the waste liquid collecting module, the waste liquid outlet is connected to the waste liquid collecting module through the sixth shut-off valve, and the on-off between the flow chamber and the waste liquid collecting module can be independently controlled through controlling the sixth shut-off valve.

Fluorescence detector (not shown in the figure)

The fluorescence detector is generally arranged near the flow chamber and comprises a laser emitter and a detector, wherein the laser emitter is used for emitting laser to the flow chamber, a sample wrapped by sheath fluid emits optical signals such as fluorescence, side scattering light, forward scattering and the like under the excitation of the laser, and the detector can capture images of the optical signals. The fluorescence detector used in this embodiment is not different from the prior art, and specific structures of the fluorescence detector including the laser emitter and the detector can be clearly and completely known to those skilled in the biological detection technology field through known technical contents.

Detection method

The utility model also provides a detection method of the flow type fluorescence detection liquid path system, which is executed by adopting the liquid path system and is used for detecting the concentration of the analyte in the sample. The detection method comprises a detection flow using a first quantitative module and a detection flow using a second quantitative module. The detection flow using the first metering module indicates that the liquid path system uses the first metering module in a sample detection process. The detection flow using the second quantification module indicates that the liquid path system used the second quantification module during a single sample detection.

The detection process using the first quantitative module specifically includes:

step1: the first multi-way valve cuts off the first quantitative module and the flow chamber, the first multi-way valve conducts the first quantitative module and the sampling device, the first quantitative module quantitatively absorbs samples through the sampling device, and the samples are stored in the first quantitative module;

step2: the first multi-way valve disconnects the first quantitative module and the sampling device, the first multi-way valve connects the first quantitative module and the flow chamber, and the first quantitative module pushes the sample to the flow chamber;

step3: the first multi-way valve is used for disconnecting the first quantitative module and the sampling device, the first multi-way valve is used for conducting the first quantitative module and the flow chamber, the sheath liquid supply module is used for pushing the sheath liquid into the flow chamber, and the fluorescence detector is used for detecting the concentration of an analyte in a sample from the first quantitative module on the flow chamber;

step 4: the first multiway valve is used for disconnecting the first quantitative module and the flow chamber, the first multiway valve is used for conducting the first quantitative module and the sampling device, and the first quantitative module is used for sucking sheath liquid through the sheath liquid supply module and cleaning the first quantitative module and the sampling device through the sheath liquid.

Similarly, the detection process using the second quantification module specifically includes:

a) The second multi-way valve cuts off the second quantifying module and the flow chamber, the second multi-way valve conducts the second quantifying module and the sampling device, the second quantifying module quantitatively absorbs the sample through the sampling device, and the sample is stored in the second quantifying module;

b) The second multi-way valve disconnects the second quantitative module and the sampling device, the second multi-way valve connects the second quantitative module and the flow chamber, and the second quantitative module pushes the sample to the flow chamber;

c) The second multi-way valve disconnects the second quantifying module and the sampling device, the second multi-way valve connects the second quantifying module and the flow chamber, the sheath liquid providing module pushes the sheath liquid into the flow chamber, and the fluorescence detector detects the concentration of the analyte in the sample from the second quantifying module on the flow chamber;

d) The second multi-way valve disconnects the second quantifying module and the flow chamber, the second multi-way valve connects the second quantifying module and the sampling device, and the second quantifying module absorbs sheath liquid through the sheath liquid providing module and cleans the second quantifying module and the sampling device through the sheath liquid.

In the detection method, the detection flow using the first quantitative module and the detection flow using the second quantitative module are simultaneously executed, so that a liquid path system can detect two samples at the same time, and the detection efficiency is greatly improved.

Since the sheath fluid supply module, the flow chamber and the sampling device are shared by the first and second dosing modules, the sheath fluid supply module, the flow chamber and the sampling device need to be used alternately by the first and second dosing modules in order to avoid the problem of contamination between samples.

To this end, in a specific embodiment, the detection method further includes:

if the first quantitative module pushes the sample to the flow chamber, the second quantitative module absorbs sheath fluid through the sheath fluid providing module and washes the first quantitative module and the sampling device through the sheath fluid. At this time, when the first quantitative module alone uses the flow chamber, the second quantitative module alone uses the sheath fluid supply module and the sampling device.

Similarly, if the second quantifying module pushes the sample to the flow chamber, the first quantifying module sucks the sheath fluid through the sheath fluid supplying module and washes the first quantifying module and the sampling device through the sheath fluid. At this time, when the second quantitative module is used alone in the flow chamber, the first quantitative module is used alone in the sheath fluid supply module and the sampling device.

In one embodiment, the detection method further includes:

if the fluorescence detector detects the concentration of the analyte in the sample from the first dosing module on the flow chamber, the second dosing module draws sheath fluid through the sheath fluid supply module and washes the first dosing module and the sampling device through the sheath fluid. At this time, when the first dosing module is used alone in the flow chamber, the second dosing module is used with the sheath fluid supply module and the sampling device.

Similarly, if the fluorescence detector detects the concentration of the analyte in the sample from the second quantification module on the flow chamber, the first quantification module draws sheath fluid through the sheath fluid supply module and washes the first quantification module and the sampling device through the sheath fluid. In this case, when the second dosing module is used alone in the flow chamber, the first dosing module is used with the sheath fluid supply module and the sampling device.

In addition, in order to improve the efficiency of the detection process using the first quantitative module and the detection process using the second quantitative module. In one embodiment, the detection method further includes:

if the fluorescence detector detects the concentration of the analyte in the sample from the first dosing module on the flow chamber, the first dosing module draws sheath fluid through the sheath fluid supply module and washes the first dosing module and the sampling device through the sheath fluid.

Further, if the fluorescence detector detects the concentration of the analyte in the sample from the first quantitative module on the flow chamber, the first quantitative module sucks the sheath fluid through the sheath fluid supply module and washes the first quantitative module and the sampling device through the sheath fluid, and the second quantitative module sucks the sheath fluid through the sheath fluid supply module and washes the second quantitative module and the sampling device through the sheath fluid.

Similarly, in a specific embodiment, the detection method further includes:

if the fluorescence detector detects the concentration of the analyte in the sample from the second quantification module on the flow chamber, the second quantification module draws sheath fluid through the sheath fluid supply module and washes the second quantification module and the sampling device through the sheath fluid.

Further, if the fluorescence detector detects the concentration of the analyte in the sample from the second quantification module on the flow chamber, the second quantification module draws the sheath fluid through the sheath fluid supply module and washes the second quantification module and the sampling device through the sheath fluid, and the first quantification module draws the sheath fluid through the sheath fluid supply module and washes the first quantification module and the sampling device through the sheath fluid.

In summary, in order to more clearly understand the beneficial effects of the above detection method, the following describes in detail the flow of the detection method for detecting the concentration of the analyte in the sample:

step1: placing the sampling device into a test tube filled with a sample, switching the first multi-way valve to conduct the sampling device and the first quantitative module, and sucking the sample by the first quantitative module through the sampling device, wherein the sample sucking time of the first quantitative module is a seconds;

step2: the method comprises the steps that a first multi-way valve is switched to conduct a first quantitative module and a flow chamber, a second multi-way valve is switched to conduct a sampling device and a second quantitative module, the first quantitative module pushes samples, the sample pushing time of the first quantitative module is b seconds, a sheath liquid providing module provides sheath liquid to the flow chamber, the sheath liquid providing time is c seconds, a sample flow wrapped by the sheath liquid is formed in the flow chamber, a fluorescence detector carries out fluorescence detection on the sample flow wrapped by the sheath liquid, the second quantitative module sucks the sheath liquid to clean the second quantitative module and the sampling device through the sheath liquid providing module, the time of the sheath liquid to clean the second quantitative module and the sampling device is d seconds, the sampling device is placed into another test tube filled with samples, the second quantitative module sucks samples through the sampling device, the sample sucking time of the second quantitative module is e seconds, c is greater than b, the sheath liquid can flush the flow chamber after the fluorescence detection is finished, so that the flow chamber can be used conveniently, and c is not less than the sum of d and e, and the second quantitative module can push samples immediately after the flow chamber is cleaned;

step3: the second multi-way valve is switched to conduct the second quantitative module and the flow chamber, the fourth shutoff valve is switched to conduct the sheath liquid supply module and the first quantitative module, the second quantitative module pushes samples, the sample pushing time f seconds of the second quantitative module, the sheath liquid supply module supplies sheath liquid to the flow chamber, the sheath liquid supply time is g seconds, a sample flow wrapped by the sheath liquid is formed in the flow chamber, the fluorescence detector carries out fluorescence detection on the sample flow wrapped by the sheath liquid, the first quantitative module absorbs the sheath liquid through the sheath liquid supply module to clean the first quantitative module and the sampling device, and the time for cleaning the first quantitative module and the sampling device by the sheath liquid is h seconds, wherein g is greater than f, and g is not less than the sum of h and a.

The iteration Step1 to Step3 can alternately detect a plurality of groups of samples, so that the detection efficiency of the plurality of groups of samples is greatly improved.

The utility model shown and described herein may be practiced without any of the elements, limitations specifically disclosed herein. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, and it is recognized that various modifications are possible within the scope of the utility model. It is therefore to be understood that while the present utility model has been specifically disclosed by various embodiments and optional features, modification and variation of the concepts herein described may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this utility model as defined by the appended claims.

The contents of articles, patents, patent applications, and all other documents, as well as the useful electronic information mentioned and cited herein, are incorporated by reference in their entirety, and any one of these publications is specifically indicated. The applicants have the right to incorporate information and material from any and all of these articles, patents, patent applications, or other documents into this application as part of the disclosure of this patent specification.

Claims (6)

1. A flow fluorescence detection fluid path system, comprising:

a flow chamber (4) comprising a sample inlet (41), a sheath fluid inlet (42) and a waste fluid outlet (43); a sheath fluid supply module (3) for supplying sheath fluid to the sheath fluid inlet (42), the first dosing module (5), and the second dosing module (6);

a sampling device (2) for providing a sample to a first quantification module (5) and a second quantification module (6);

the first quantitative module (5) is used for sucking and cleaning the sheath liquid of the first quantitative module (5) and the sampling device (2) through the sheath liquid supply module (3) and is also used for quantitatively sucking a sample through the sampling device (2);

a second quantifying module (6) for sucking and cleaning the sheath liquid of the second quantifying module (6) and the sampling device (2) by the sheath liquid supply module (3), and for quantitatively sucking the sample by the sampling device (2);

the sheath fluid supply module (3) is connected to the sheath fluid inlet (42), the first quantitative module (5) and the second quantitative module (6) through the first valve group;

and the second valve group is used for connecting the first quantitative module (5) to the sample inlet (41) and the sampling device (2), and the second quantitative module (6) is connected to the sample inlet (41) and the sampling device (2) through the second valve group.

2. A flow fluorescence detection fluid path system according to claim 1, wherein the first valve set comprises a third shut-off valve (9), the sheath fluid supply module (3) is connected to the first dosing module (5) via the third shut-off valve (9), the first valve set comprises a fourth shut-off valve (10), and the sheath fluid supply module (3) is connected to the second dosing module (6) via the fourth shut-off valve (10).

3. A flow-through fluorescence detection fluid circuit system according to claim 1, wherein the second valve set comprises a first multiplex valve (7), and the first metering module (5) is connected to the sample inlet (41) and the sampling device (2) via the first multiplex valve (7).

4. A flow fluorescence detection fluid path system according to claim 1 or 3, wherein the second valve set comprises a second multiplex valve (8), and the second dosing module (6) is connected to the sample inlet (41) and the sampling device (2) via the second multiplex valve (8).

5. A flow fluorescence detection fluid path system according to claim 1, wherein the first dosing module (5) comprises two first fluid ports (51), the first valve set and the second valve set being connected to the two first fluid ports (51), respectively, and the second dosing module (6) comprises two second fluid ports (61), the first valve set and the second valve set being connected to the two second fluid ports (61), respectively.

6. A flow fluorescence detection fluid path system according to claim 1, further comprising a waste fluid collector and a sixth shut-off valve (12), the waste fluid collector being connected to the waste fluid outlet (43) via the sixth shut-off valve (12).

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