CN211718128U - Antioxidant screening tester - Google Patents
Antioxidant screening tester Download PDFInfo
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- CN211718128U CN211718128U CN202020100227.4U CN202020100227U CN211718128U CN 211718128 U CN211718128 U CN 211718128U CN 202020100227 U CN202020100227 U CN 202020100227U CN 211718128 U CN211718128 U CN 211718128U
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Abstract
An antioxidant screening tester comprises a conveying box, a detection box, a column temperature box and a constant flow box group which are sequentially distributed from top to bottom, wherein a container assembly for containing solution is placed at the top of the conveying box; a first peristaltic pump and a second peristaltic pump for conveying the solution are arranged in the conveying box; the technical key points are that an integral machine for screening antioxidant and evaluating antioxidant capacity is designed, and a peristaltic pump, a chemiluminescence detector and a high performance liquid chromatography separation system are integrated together; the total antioxidant capacity can be evaluated by using the chemiluminescence detector part alone, and the antioxidant activity of a single component in a mixture can be evaluated by combining a chromatographic separation device in a constant flow box group; the manufactured whole machine is compact, the space utilization and the pipeline design layout are reasonable, the operation is easy, the six-way sample injection valve using the flow injection luminometer is reduced, and the cost is saved; has wide application prospect in the fields of traditional Chinese medicine and food research and development and detection.
Description
Technical Field
The utility model belongs to the technical field of the sample test, specifically an anti-oxide screening apparatus.
Background
Screening and evaluation research of antioxidants with free radical scavenging capacity in natural products increasingly become hot, and the traditional method for screening and evaluating antioxidant active substances is to separate chemical components one by one, identify the chemical components by means of liquid quality and spectrum qualitative means, and evaluate the antioxidant property of the antioxidant substances, and has the disadvantages of complex steps and time consumption.
At present, the high performance liquid chromatography separation and the online chemiluminescence method are adopted to realize the screening of antioxidant substances, but no commercialized instrument is available, and most methods report that the high performance liquid chromatograph is used in combination with a flow injection chemiluminescence apparatus or the high performance liquid chromatograph is used in combination with a peristaltic pump and an ultra-weak luminescence tester; although the combined instruments can realize the on-line real-time screening and determination of the antioxidant, the instruments all need two pieces of operation software, are inconvenient to operate, have more and scattered components, large floor area and high manufacturing cost.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome prior art's defect, provide an antioxidant screening apparatus.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
an antioxidant screening tester comprises a conveying box, a detection box, a column temperature box and a constant flow box group which are sequentially distributed from top to bottom, wherein the top of the conveying box is provided with a container component for containing solution,
a first peristaltic pump and a second peristaltic pump for conveying solution are arranged in the conveying box;
a chemiluminescence detector is arranged in the detection box and comprises a flow cell disc and a photomultiplier, and an inlet and an outlet formed in the flow cell disc are respectively communicated with the first peristaltic pump and the second peristaltic pump;
a chromatographic column is arranged in the column temperature box, a six-way sample injection valve is arranged on one side of the column temperature box, and the whole column temperature box is used for accurately controlling the separation temperature;
the constant flow box group comprises a first constant flow box and a second constant flow box, a first chromatographic pump and a second chromatographic pump are respectively arranged in the first constant flow box and the second constant flow box, the output ends of the first chromatographic pump and the second chromatographic pump are communicated with the six-way sampling valve through a mixer, and the mixer is arranged on one side of the constant flow box group;
the container assembly comprises a first solution bottle, a second solution bottle, a waste liquid bottle, a sample bottle and a reagent bottle.
Preferably, the second peristaltic pump is connected with the flow cell disc through a first pipeline and a second pipeline which are sequentially connected, an output pipeline matched with the chromatographic column and an output pipeline matched with the first peristaltic pump are connected with the connecting ends of the first pipeline and the second pipeline through a second three-way valve, and the connecting end of the first pipeline and the second peristaltic pump is provided with the first three-way valve; the second peristaltic pump is communicated with the reagent bottle.
Preferably, the first pipeline and the second pipeline both adopt telescopic hoses, and the first peristaltic pump and the second peristaltic pump are identical in specification.
Preferably, the output pipeline matched with the first peristaltic pump is communicated with the waste liquid bottle, and the other end of the matched output pipeline is communicated with the chromatographic column after waste liquid is collected by the flow cell disc; the input pipeline matched with the first peristaltic pump is communicated with the sample bottle, and the other end of the input pipeline matched with the first peristaltic pump is communicated with the second three-way valve.
Preferably, the flow cell disc is made of glass materials and is designed into a disc shape, the photomultiplier is installed at the rear side position of the flow cell disc, and the disc ports of the flow cell disc and the sensitive windows of the photomultiplier are distributed oppositely.
Preferably, the measuring instrument is connected with a computer through a data line, a workstation is loaded in the computer, parameters and states of the measuring instrument are controlled by the workstation, and data processing and display of the measuring instrument are completed by the workstation.
Compared with the prior art, the utility model provides an anti-oxide screening apparatus has following beneficial effect:
the utility model designs an integrated machine for screening antioxidant and evaluating antioxidant ability, which integrates a peristaltic pump, a chemiluminescence detector and a high performance liquid chromatography separation system; the total antioxidant capacity can be evaluated by using the chemiluminescence detector part alone, and the antioxidant activity of a single component in a mixture can be evaluated by combining a chromatographic separation device in a constant flow box group; the manufactured whole machine is compact, the space utilization and the pipeline design layout are reasonable, the operation is easy, the six-way sample injection valve using the flow injection luminometer is reduced, and the cost is saved; has wide application prospect in the fields of traditional Chinese medicine and food research and development and detection;
the chemiluminescence detection method is combined with the HPLC technology, so that the on-line identification, screening and activity evaluation of antioxidant substances can be realized, the defect that the conventional evaluation method is time-consuming and tedious in operation is overcome, and the sensitivity and accuracy of free radical identification are improved.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a cross-sectional view of the overall structure of the present invention;
fig. 3 is a schematic view of a partial structure of fig. 2 according to the present invention.
Reference numerals: 1. a delivery box; 2. a detection box; 3. a column oven; 4. a first constant current box; 5. a second constant current box; 6. a blending machine; 7. a six-way sampling valve; 8. a container assembly; 81. a first solution bottle; 82. a second solution bottle; 83. a waste liquid bottle; 84. a sample bottle; 85. a reagent bottle; 9. a peristaltic pump No. one; 10. a peristaltic pump II; 11. a flow cell tray; 12. a chromatographic column; 13. a chromatography pump number one; 14. a second chromatography pump; 15. a first three-way valve; 16. a second three-way valve; 17. a first pipeline; 18. a second pipeline; 19. a photomultiplier tube.
Detailed Description
The following description will further describe a specific embodiment of the antioxidant screening apparatus of the present invention with reference to fig. 1. The present invention is not limited to the description of the following examples.
Example 1:
the embodiment provides a specific structure of an antioxidant screening tester, and as shown in fig. 1-3, the antioxidant screening tester comprises a conveying box 1, a detection box 2, a column temperature box 3 and a constant flow box group which are sequentially distributed from top to bottom, wherein a container assembly 8 for containing solution is placed at the top of the conveying box 1, and a first peristaltic pump 9 and a second peristaltic pump 10 for conveying solution are arranged in the conveying box 1;
a chemiluminescence detector is arranged in the detection box 2 and comprises a flow cell disc 11 and a photomultiplier 19, and inlet and outlet ports formed in the flow cell disc 11 are respectively communicated with the first peristaltic pump 9 and the second peristaltic pump 10;
the chemiluminescence detector mentioned here is a component of a high performance liquid chromatograph LC-20A, and the detection box 2, the column incubator 3 and the constant flow box group all belong to components of the high performance liquid chromatograph LC-20A.
A chromatographic column 12 is arranged in the column temperature box 3, a six-way sample injection valve 7 is arranged on one side of the column temperature box 3, and the whole column temperature box 3 is used for accurately controlling the separation temperature;
the constant flow box group comprises a first constant flow box 4 and a second constant flow box 5, a first chromatographic pump 13 and a second chromatographic pump 14 are respectively arranged in the first constant flow box 4 and the second constant flow box 5, the output ends of the first chromatographic pump 13 and the second chromatographic pump 14 are communicated with a six-way sample injection valve 7 through a mixer 6, and the mixer 6 is arranged on one side of the constant flow box group;
the container assembly 8 includes a first solution bottle 81, a second solution bottle 82, a waste liquid bottle 83, a sample bottle 84, and a reagent bottle 85.
As shown in fig. 2 and 3, the first pipeline 17 and the second pipeline 18 which are connected in sequence are arranged between the second peristaltic pump 10 and the flow cell disc 11, the output pipeline matched with the chromatographic column 12 and the output pipeline matched with the first peristaltic pump 9 are connected with the connecting ends of the first pipeline 17 and the second pipeline 18 by a second three-way valve 16, and the connecting end of the first pipeline 17 and the second peristaltic pump 10 is provided with a first three-way valve 15; the second peristaltic pump 10 is communicated with the reagent bottle 85.
As shown in FIG. 3, the first pipeline 17 and the second pipeline 18 both adopt flexible hoses, and the first peristaltic pump 9 and the second peristaltic pump 10 have the same specification.
As shown in fig. 2 and 3, an output pipeline matched with the first peristaltic pump 9 is communicated with a waste liquid bottle 83, and the other end of the matched output pipeline is communicated with a chromatographic column 12 after waste liquid is collected by a flow cell disc 11; the input pipeline matched with the first peristaltic pump 9 is communicated with the sample bottle 84, and the other end of the matched input pipeline is communicated with the second three-way valve 16.
As shown in fig. 3, the flow cell plate 11 is made of glass material and is designed into a coil shape, the photomultiplier tube 19 is installed at a rear position of the flow cell plate 11, and the disc ports of the flow cell plate 11 are distributed opposite to the sensitive window of the photomultiplier tube 19.
The measuring instrument is connected with a computer through a data line, a workstation is loaded in the computer, the parameters and the state of the measuring instrument are controlled by the workstation, and the data processing and the display of the measuring instrument are completed by the workstation.
By adopting the technical scheme, an integrated machine for screening antioxidant and evaluating antioxidant capacity is designed, and a peristaltic pump, a chemiluminescence detector and a high performance liquid chromatography separation system are integrated together; the total antioxidant capacity can be evaluated by using the chemiluminescence detector part alone, and the antioxidant activity of a single component in a mixture can be evaluated by combining a chromatographic separation device in a constant flow box group; the manufactured whole machine is compact, the space utilization and the pipeline design layout are reasonable, the operation is easy, the six-way sample injection valve using the flow injection luminometer is reduced, and the cost is saved; has wide application prospect in the fields of traditional Chinese medicine and food research and development and detection.
Example 2:
this example shows the specific working steps of each box of an antioxidant screening tester: as shown in fig. 2, two chromatographic pumps in the constant flow box group at the lowest part of the apparatus are liquid chromatographic constant flow pumps, and a high-pressure gradient elution mode is adopted, so that the flow rate can be accurately controlled;
the column temperature box 3 is arranged above the constant flow box group, so that the accurate control of the separation temperature is facilitated;
a detection box 2 is arranged above the column incubator 3, and a chemiluminescence detector arranged in the detection box comprises a flow cell plate 11 and a photomultiplier 19; the flow cell disc 11 is made of glass materials, is low in cost and beneficial to light transmission, and is designed into a coil form so as to ensure that chemical reaction is fully performed and the maximum luminous signal is obtained; the photomultiplier 19 is arranged behind the flow cell disc 11, and the disc of the flow cell disc 11 is opposite to the sensitive window of the photomultiplier 19, so that the space is saved;
the uppermost part of the instrument is a conveying box 1 for conveying luminescent reagents, two peristaltic pumps are arranged in the instrument, a pump tube is a silicone tube, a second peristaltic pump 10 is used, a plurality of three-way valves are matched for mixing a plurality of chemical reagents in each reagent bottle 85 one by one, the mixed solution meets the effluent liquid of the chromatographic column 12 in a second three-way valve 16 after passing through a first pipeline 17 with the length of L2, and the mixed solution enters a flow cell tray 11 after passing through a second pipeline 18 with the length of L1; because the first pipeline 17 and the second pipeline 18 both adopt telescopic pipes, the lengths of L1 and L2 can be adjusted according to the type and speed of the chemiluminescence reaction, so that the generated luminescence signals are optimal; then, the waste liquid flowing out of the flow cell disc 11 is pumped by a peristaltic pump I and enters a waste liquid bottle 83;
when the chemiluminescence detection device is used alone to evaluate the total antioxidant activity, the peristaltic pump 9I can be used to convey the sample solution in the sample bottle 84, and the sample solution meets the chemiluminescence reagent in the three-way valve 16 II, enters the flow cell tray 11, and then the detection of the luminescence signal is carried out. The measuring instrument is connected with a computer through a data line, a workstation is loaded in the computer, the parameters and the state of the measuring instrument are controlled by the workstation, and the data processing and the display of the measuring instrument are completed by the workstation.
The foregoing is a more detailed description of the present invention, taken in conjunction with the specific preferred embodiments thereof, and it is not intended that the invention be limited to the specific embodiments shown and described. To the utility model belongs to the technical field of ordinary technical personnel, do not deviate from the utility model discloses under the prerequisite of design, can also make a plurality of simple deductions or replacement, all should regard as belonging to the utility model discloses a protection scope.
Claims (6)
1. The utility model provides an anti oxide screening apparatus, this apparatus include from the top that distributes in proper order transport case (1), detection case (2), column incubator (3) and constant current case group that set up, container subassembly (8) that are used for holding solution, its characterized in that have been placed to the top of transport case (1):
a first peristaltic pump (9) and a second peristaltic pump (10) for conveying solutions are arranged in the conveying box (1);
a chemiluminescence detector is arranged in the detection box (2) and comprises a flow cell disc (11) and a photomultiplier (19), and inlet and outlet ports formed in the flow cell disc (11) are respectively communicated with a first peristaltic pump (9) and a second peristaltic pump (10);
a chromatographic column (12) is arranged in the column temperature box (3), a six-way sampling valve (7) is arranged on one side of the column temperature box (3), and the whole column temperature box (3) is used for accurately controlling the separation temperature;
the constant flow box group comprises a first constant flow box (4) and a second constant flow box (5), a first chromatographic pump (13) and a second chromatographic pump (14) are respectively arranged in the first constant flow box (4) and the second constant flow box (5), the output ends of the first chromatographic pump (13) and the second chromatographic pump (14) are communicated with a six-way sample injection valve (7) through a mixer (6), and the mixer (6) is arranged on one side of the constant flow box group;
the container assembly (8) comprises a first solution bottle (81), a second solution bottle (82), a waste liquid bottle (83), a sample bottle (84) and a reagent bottle (85).
2. The antioxidant screening assay of claim 1, wherein: the second peristaltic pump (10) is connected with the flow cell disc (11) through a first pipeline (17) and a second pipeline (18) which are sequentially connected, an output pipeline matched with the chromatographic column (12) and an output pipeline matched with the first peristaltic pump (9) are connected with the connecting ends of the first pipeline (17) and the second pipeline (18) through a second three-way valve (16), and a first three-way valve (15) is arranged at the connecting end of the first pipeline (17) and the second peristaltic pump (10); the second peristaltic pump (10) is communicated with the reagent bottle (85).
3. The antioxidant screening assay of claim 2, wherein: the first pipeline (17) and the second pipeline (18) both adopt telescopic hoses, and the first peristaltic pump (9) and the second peristaltic pump (10) are identical in specification.
4. The antioxidant screening assay of claim 1, wherein: an output pipeline matched with the first peristaltic pump (9) is communicated with a waste liquid bottle (83), and the other end of the matched output pipeline is communicated with the chromatographic column (12) after waste liquid is collected by a flow cell disc (11); an input pipeline matched with the first peristaltic pump (9) is communicated with the sample bottle (84), and the other end of the matched input pipeline is communicated with the second three-way valve (16).
5. The antioxidant screening assay of claim 1, wherein: the flow cell disc (11) is made of glass materials and designed into a disc shape, the photomultiplier (19) is installed at the rear side position of the flow cell disc (11), and the disc ports of the flow cell disc (11) and the sensitive windows of the photomultiplier (19) are distributed oppositely.
6. The antioxidant screening assay of claim 1, wherein: the measuring instrument is connected with a computer through a data line, a workstation is loaded in the computer, parameters and states of the measuring instrument are controlled by the workstation, and data processing and display of the measuring instrument are completed by the workstation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020100227.4U CN211718128U (en) | 2020-01-17 | 2020-01-17 | Antioxidant screening tester |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020100227.4U CN211718128U (en) | 2020-01-17 | 2020-01-17 | Antioxidant screening tester |
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| Publication Number | Publication Date |
|---|---|
| CN211718128U true CN211718128U (en) | 2020-10-20 |
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| CN202020100227.4U Active CN211718128U (en) | 2020-01-17 | 2020-01-17 | Antioxidant screening tester |
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| CN (1) | CN211718128U (en) |
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- 2020-01-17 CN CN202020100227.4U patent/CN211718128U/en active Active
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