CN115615880A - Sample analyzer - Google Patents
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- CN115615880A CN115615880A CN202110807494.4A CN202110807494A CN115615880A CN 115615880 A CN115615880 A CN 115615880A CN 202110807494 A CN202110807494 A CN 202110807494A CN 115615880 A CN115615880 A CN 115615880A
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Abstract
The invention is suitable for the technical field of medical equipment, and discloses a sample analyzer which comprises a sampling device, a liquid path supporting device, a blood routine detection device and a blood sedimentation detection device, wherein the sampling device comprises a sampling needle, a sample suction pipeline and a motion driving device, and the sampling needle is provided with a sample suction port far away from the sample suction pipeline; the blood sedimentation detection device comprises a blood sedimentation detection assembly, one part of the sample suction pipeline is used as a blood sedimentation detection pipe section, the sampling needle can move relative to the blood sedimentation detection assembly under the driving of the movement driving device, and the volume of a fluid channel between the blood sedimentation detection pipe section and the sample suction port is less than or equal to 200 muL. The sample analyzer provided by the invention integrates the conventional blood detection function and the blood sedimentation detection function; and the setting position of the blood sedimentation detection assembly is more flexible and is not limited by the structure of the sampling device, and the technical problem of low accuracy of the blood sedimentation detection result caused by unreasonable structural design of the blood sedimentation detection device and the sampling device is effectively solved.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a sample analyzer.
Background
The sample analyzer provided by the prior art integrates a blood routine detection function and a blood sedimentation detection function, that is, the sample analyzer includes a blood routine detection device and a blood sedimentation detection device, wherein the blood routine detection device is used for performing blood routine detection on a blood sample, and the blood sedimentation detection device is used for performing Erythrocyte Sedimentation Rate (ESR) detection on the blood sample. The sampling and sample dividing mode of the sample analyzer is as follows: before sampling, the sampling device is filled with diluent in a sampling needle and a sample suction pipeline; after the sampling device samples, the blood sample needs to be respectively distributed to a blood routine detection device and a blood sedimentation detection device for detection. The structural design of the existing sample analyzer is not reasonable enough, so that the blood sample distributed to the blood sedimentation detection device is easily diluted by diluent or greatly lost in a sample sucking pipeline, and the accuracy of the blood sedimentation detection result is low.
Disclosure of Invention
The invention aims to provide a sample analyzer, which aims to solve the technical problem that the accuracy of a blood sedimentation detection result is low due to unreasonable structural designs of a blood sedimentation detection device and a sampling device in the prior art.
In order to achieve the purpose, the invention provides the following scheme: a sample analyzer, comprising:
the sampling device comprises a sampling needle, a sample suction pipeline connected with the sampling needle and a motion driving device for driving the sampling needle to move, wherein the sampling needle is provided with a sample suction port far away from the sample suction pipeline;
the liquid path supporting device comprises a fluid driving device which is communicated with the sample sucking pipeline and is used for providing driving force for the fluid to flow in the sampling needle and the sample sucking pipeline;
the blood routine detection device comprises a detection pool and a blood routine detection assembly, the detection pool is used for providing a blood routine detection place for the blood sample to be detected distributed by the sampling device, and the blood routine detection assembly is used for performing blood routine detection on the blood sample to be detected in the detection pool;
the blood sedimentation detection device comprises a blood sedimentation detection assembly, one part of the sample suction pipeline is used as a blood sedimentation detection pipeline section, the blood sedimentation detection pipeline section is used for providing a blood sedimentation detection place for a blood sample to be detected, and the blood sedimentation detection assembly is used for performing blood sedimentation detection on the blood sample to be detected in the blood sedimentation detection pipeline section;
the sampling needle can move relative to the blood sedimentation detection assembly under the driving of the movement driving device, and the volume of a fluid channel between the blood sedimentation detection pipe section and the sample suction port is less than or equal to 200 mu L.
A second object of the present invention is to provide a sample analyzer, comprising:
the sampling device comprises a sampling needle, a sample sucking pipeline connected with the sampling needle and a motion driving device used for driving the sampling needle to move, wherein the sampling needle is provided with a sample sucking port far away from the sample sucking pipeline;
the liquid path supporting device comprises a fluid driving device which is communicated with the sample sucking pipeline and is used for providing driving force for the fluid to flow in the sampling needle and the sample sucking pipeline;
the blood routine detection device comprises a detection pool and a blood routine detection assembly, the detection pool is used for providing a blood routine detection place for the blood sample to be detected distributed by the sampling device, and the blood routine detection assembly is used for performing blood routine detection on the blood sample to be detected in the detection pool;
the blood sedimentation detection device comprises a blood sedimentation detection assembly, one part of the sample suction pipeline is used as a blood sedimentation detection pipeline section, the blood sedimentation detection pipeline section is used for providing a blood sedimentation detection place for a blood sample to be detected, and the blood sedimentation detection assembly is used for performing blood sedimentation detection on the blood sample to be detected in the blood sedimentation detection pipeline section;
the sampling needle can move relative to the blood sedimentation detection assembly under the driving of the movement driving device;
the sample analyzer is configured to: before the sampling needle collects the blood sample to be detected, diluent is filled in the sampling needle and the sample suction pipeline; after the sampling needle collects the blood sample to be detected, more than two sections of isolation gas columns which are arranged at intervals are formed between the diluent and the blood sample to be detected, and one section of isolation gas column is formed between any two adjacent sections of isolation gas columns.
The invention has the beneficial effects that:
the invention provides a sample analyzer, which can make the setting position of the blood sedimentation detecting component more flexible without being limited by the structure of the sampling device by designing the blood sedimentation detecting component and the sampling needle to be capable of relative movement. In addition, a part of the sample suction pipeline is used as a blood sedimentation detection pipeline section, and the volume of a fluid channel between the blood sedimentation detection pipeline section and a sample suction port of the sampling needle is designed to be less than or equal to 200 mu L, so that when a blood sample to be detected is driven and conveyed to the blood sedimentation detection pipeline section, the dilution degree of the blood sample to be detected is small, the blood sample to be detected lost in the sample suction pipeline is small, and the precision of blood sedimentation detection is effectively guaranteed.
The sample analyzer provided by the second object of the invention can make the setting position of the blood sedimentation detecting component more flexible without being limited by the structure of the sampling device by designing the blood sedimentation detecting component and the sampling needle to be capable of relative movement. Further, it is achieved by using a portion of the sample withdrawal line as a sedimentation detection tubing section, and configuring the sample analyzer to: after the sampling needle gathered the blood sample that awaits measuring, be formed with the isolation gas column that the interval set up more than two sections between the diluent and the blood sample that awaits measuring, and be formed with one section isolation blood sample between arbitrary adjacent two sections isolation gas columns, thus, through the setting of keeping apart the blood sample, make the blood sample that awaits measuring the total volume of blood sample that awaits measuring when long distance transport to the blood sedimentation detection pipeline section remain unchanged basically, and make the total amount of diluent that finally sneak into in the blood sample that awaits measuring far be less than the total residual quantity of diluent at the suction sample pipeline pipe wall, the dilution influence of diluent to the blood sample that awaits measuring has greatly been reduced, effectively solved because of the blood sedimentation detection pipeline section and the overlength of sampling needle suction mouth bring the problem of blood sample dilution that awaits measuring, and then also effectively ensured the precision of blood sedimentation detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a system block diagram of a sample analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a system for holding a sample analyzer in a standby position according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a system for sampling a sample analyzer according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a system for separating a blood sample from a conventional blood testing device by using a sample analyzer according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a system for analyzing a sample analyzer in a blood sedimentation measurement state according to an embodiment of the present invention;
FIG. 6 is a schematic diagram illustrating a state of a blood sample being tested being pulled to flow to a section of a sedimentation test tube according to an embodiment of the present invention;
FIG. 7 is a diagram illustrating a state of a blood sample to be tested being drawn to flow into a section of a sedimentation test tube according to a second embodiment of the present invention;
FIG. 8 is a schematic diagram of a system of a sample analyzer in a sample sucking state according to a third embodiment of the present invention;
fig. 9 is a schematic view of a state in which a first tube switching member and a syringe in a fluid path supporting apparatus according to a third embodiment of the present invention are sucking;
FIG. 10 is a schematic view of the first tube switching part and the syringe in the liquid path supporting apparatus according to the third embodiment of the present invention in a liquid discharge state;
FIG. 11 is a schematic diagram of a state in which a blood sample to be tested is dragged to flow to a section of a sedimentation test tube according to a third embodiment of the present invention;
fig. 12 is a schematic diagram of a system in which a sample analyzer is in a standby state according to a fourth embodiment of the present invention.
The reference numbers illustrate:
100. a sample analyzer; 110. a sampling device; 111. a sampling needle; 1111. a sample sucking port; 112. a motion drive device; 1121. a transverse guide rail; 1122. a lateral motion assembly; 1123. a vertical guide rail; 1124. a vertical motion assembly; 113. a sample suction pipeline; 1131. a blood sedimentation detection section; 1132. a first sample suction pipe section; 1133. a second sample suction pipe section; 1134. a main branch; 1135. a first branch; 1136. a second branch circuit; 114. a second pipeline switching part; 120. a liquid path supporting device; 121. a fluid driving device; 1211. an injector; 1212. a first power unit; 1213. a second power unit; 122. a drainage line; 123. a first pipeline switching part; 1231. a first three-way valve; 130. a blood routine test device; 131. a detection cell; 140. a blood sedimentation detection device; 141. a sedimentation detection component; 1411. a light source; 1412. a photoelectric converter; 1413. a heater; 1414. a temperature sensor; 142. a fixing member; 150. diluting the solution; 160. blood sample to be tested; 170. isolating the gas column; 171. a first isolated gas column; 172. a second isolated gas column; 180. isolating the blood sample; 190. a sample container.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all directional indications (such as up, down, left, right, front, back \8230;) in the embodiments of the present invention are only used to explain the relative positional relationship between the components in a particular pose, the motion, etc., and if the particular pose is changed, the directional indication is correspondingly changed.
It will also be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or be indirectly connected to the other element through intervening elements.
In addition, the descriptions related to "first", "second", etc. in the present invention are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
The first embodiment is as follows:
as shown in fig. 1-6, a sample analyzer 100 according to an embodiment of the present invention includes a sampling device 110, a fluid circuit supporting device 120, a blood routine detecting device 130, and a blood sedimentation detecting device 140. The sampling device 110 is used for sucking and separating the blood sample 160 to be tested, i.e. for sucking the blood sample 160 to be tested and distributing the blood sample 160 to be tested to the blood routine testing device 130 and the blood sedimentation testing device 140. The blood routine testing device 130 is used for performing blood routine tests on the blood sample 160 to be tested, which may include, but is not limited to, leucocyte differential count tests, reticulocyte tests, hemoglobin test, red blood cell count tests, platelet count tests, and the like. The sedimentation detection apparatus 140 is used for detecting the sedimentation rate of red blood cells of the blood sample 160 to be tested. The fluid circuit support device 120 is used for providing fluid circuit support for the sampling device 110, the blood sedimentation detection device 140 and the blood routine detection device 130 respectively; fluid circuit support may include functional support for fluid actuation, reagent filling, fluid circuit cleaning, waste fluid removal, and the like.
The sampling device 110 includes a sampling needle 111, a sample suction pipeline 113 connected to the sampling needle 111, and a movement driving device 112 for driving the sampling needle 111 to move, wherein the sampling needle 111 has a sample suction port 1111 far from the sample suction pipeline 113. The motion driving device 112 is connected to the sampling needle 111, and is configured to drive the sampling needle 111 to move in a spatial dimension along a predetermined direction, so as to move the sampling needle 111 to a different working position, such as a standby position, a sampling position, a sample splitting position, and the like. The liquid path/liquid path supporting device 120 is connected to the sampling line, and can drive fluid such as liquid, gas, etc. to flow in the sample suction needle and the sample suction line 113.
The motion driving device 112 provided by the present embodiment includes a lateral guide rail 1121, a lateral motion assembly 1122, a lateral driving assembly, a vertical guide rail 1123, a vertical motion assembly 1124, and a vertical driving assembly. Wherein, sampling needle 111 installs on vertical motion subassembly 1124, and vertical guide rail 1123 installation lateral motion subassembly 1122 or integrated into one piece are on lateral motion subassembly 1122, and vertical motion subassembly 1124 liftable is installed on vertical guide rail 1123 with sliding, and vertical drive assembly installs on lateral motion subassembly 1122 and is connected with vertical motion subassembly 1124, and vertical drive assembly is used for driving vertical motion subassembly 1124 to drive sampling needle 111 and carries out elevating movement. The lateral moving assembly 1122 is transversely slidably mounted on the lateral guide rail 1121, and the lateral driving assembly is connected to the lateral moving assembly 1122 so as to drive the lateral moving assembly 1122 to drive the vertical guide rail 1123, the vertical moving assembly 1124, the vertical driving assembly and the sampling needle 111 to move laterally together. In a specific application, the lateral moving component 1122 may drive the sampling needle 111 to slide above the sample container 190 containing the blood sample 160 or the blood routine testing device 130, or may drive the sampling needle 111 to move away from the sample container 190 containing the blood sample 160 or the blood routine testing device 130. The vertical motion assembly 1124 can drive the sampling needle 111 to descend and extend into the sample container 190 containing the blood sample 160 to be detected to absorb the blood sample 160 to be detected, and after the sample absorption is finished, the vertical motion assembly 1124 can drive the sampling needle 111 to ascend to the upper part of the sample container 190; the vertical motion component 1124 can also drive the sampling needle 111 to descend to a position suitable for injecting the blood sample 160 to be detected into the blood routine detecting device 130, and after the sample division is completed, the vertical motion component 1124 can drive the sampling needle 111 to ascend to the upper part of the sample division position. Of course, in a specific application, the arrangement of the motion driving device 112 is not limited to this, for example, as an alternative embodiment, the sampling needle 111 may be mounted on the lateral motion assembly 1122, and the lateral guide 1121 and the lateral driving device may be arranged on the vertical motion assembly 1124; or, as another alternative embodiment, the motion driving device 112 may further include a longitudinal guide rail, a longitudinal motion assembly, and a longitudinal driving assembly on the basis of including the transverse guide rail 1121, the transverse motion assembly 1122, the transverse driving assembly, the vertical guide rail 1123, the vertical motion assembly 1124, and the vertical driving assembly, where the longitudinal guide rail and the transverse guide rail 1121 are two horizontal directions perpendicular to each other, that is, the sampling needle 111 may perform a motion in a three-dimensional direction; still alternatively, as yet another alternative embodiment, the motion driving device 112 may employ a combination of a swing arm rotation mechanism and a lifting mechanism.
The fluid path supporting device 120 includes a fluid driving device 121, and the fluid driving device 121 is communicated with the sample suction pipeline 113 to provide a driving force for the fluid flowing in the sampling needle 111 and the sample suction pipeline 113. The flow driving device is a power device which can provide positive pressure and negative pressure. During sampling, the flow driving device provides a negative pressure to draw the blood sample 160 of the sample container 190 to be tested into the sampling needle 111. Upon dispensing the test blood sample 160 into the blood routine testing device 130, the flow driving device may provide a positive pressure to expel the test blood sample 160 for dispensing into the blood routine testing device 130; alternatively, when the blood sample 160 is dispensed to the blood routine testing device 130, the flow driving device may provide a negative pressure to draw the blood sample 160 into a section of the tube, and then provide a positive pressure to push the blood sample 160 into the blood routine testing device 130. Upon dispensing the sample of blood 160 to be tested to the blood sedimentation device 140, the flow driving device may provide a negative pressure to draw the sample of blood 160 to be tested into the blood sedimentation line. Of course, in a specific application, the fluid path supporting device 120 may include not only the fluid driving device 121, but also a pipeline, a container and a power for providing fluid path support for the functions of reagent filling, diluent 150 delivery, fluid path cleaning, waste fluid discharge, and the like, for example, the fluid path supporting device 120 may also provide cleaning solutions for the sampling device 110, the blood sedimentation detection device 140 and the blood routine detection device 130, respectively, so as to clean the sampling needle 111, the sample suction pipeline 113, the blood sedimentation detection device 140 and the blood routine detection device 130, respectively, and avoid polluting the blood sample 160 to be detected and causing inaccurate detection results; still alternatively, the liquid path supporting device 120 may fill the sample sucking needle and the sample sucking pipeline 113 with the diluent 150 before sampling, so that the processes of sampling, sample splitting, and the like can be realized more quickly and reliably.
As an implementation manner, the fluid driving device 121 adopted in the present embodiment is an injector 1211, which does not need to design a complicated and costly air path system, and is beneficial to realizing a low-cost and miniaturized design of the sample analyzer 100. Of course, in particular applications, the fluid drive device 121 is not limited to use with a syringe 1211 and, for example, as an alternative, a fixed displacement pump or other pressure source capable of providing positive and negative power, such as positive and negative air pressure, may be used. In a preferred embodiment, the volume of the syringe 1211 used in the fluid driving device 121 is 500 μ L or less, so that the volume of the syringe 1211 can be reduced, thereby facilitating the accuracy of the blood sample dispensed to the blood routine testing device 130. Of course, in alternative embodiments, the volume of the syringe 1211 used in the fluid driving device 121 may be greater than 500 μ L in specific applications.
The blood routine testing device 130 includes a testing cell 131 and a blood routine testing component (not shown), the testing cell 131 is used for providing a blood routine testing place for the blood sample 160 to be tested distributed by the sampling device 110, and the blood routine testing component is used for performing blood routine testing on the blood sample 160 to be tested in the testing cell 131. The number of the detection cells 131 and the number of the blood routine detection components are at least one, or a plurality of detection cells 131, and the detection cells 131 can provide detection sites for detection of different parameters, and different detection components can detect blood routine parameters such as red blood cells, white blood cells, hemoglobin by methods such as impedance method, colorimetric method, optical method, and the like, for example, one detection cell 131 and a corresponding detection component are provided for white blood cell differential counting and reticulocyte detection, one detection cell 131 and a corresponding detection component are provided for hemoglobin detection, and one detection cell 131 and a corresponding detection component are provided for red blood cell number detection and/or platelet counting detection. Of course, the blood routine detecting device 130 may further include a reaction cell for providing a reaction site for the blood sample 160 to be detected, the blood sample 160 to be detected collected by the sampling device 110 is distributed to the corresponding reaction cell, the detection cell 131 and the reaction cell of some detection items are set independently of each other, and the detection cell 131 and the reaction cell of some detection items are set integrally, for example, the detection cell 131 and the reaction cell of the differential white blood cell count detection are set independently of each other, and the detection cell 131 and the reaction cell of hemoglobin are integrated.
The blood sedimentation detecting device 140 comprises a blood sedimentation detecting component 141, a part of the sample suction pipeline 113 is used as a blood sedimentation detecting pipe section 1131, the blood sedimentation detecting pipe section 1131 is used for providing a blood sedimentation detecting place for the blood sample 160 to be detected, and the blood sedimentation detecting component 141 is used for performing blood sedimentation detection on the blood sample 160 to be detected in the blood sedimentation detecting pipe section 1131. The blood sedimentation detection assembly 141 can obtain information on the speed or degree of red blood cell aggregation by performing light absorption or light scattering measurements on the blood sample 160 to be tested in the blood sedimentation detection tube section 1131 in exchange for an ESR value. The sampling needle 111 can move relative to the blood sedimentation test component 141 under the driving of the movement driving device 112, and the volume of the fluid channel between the blood sedimentation test tube section 1131 and the sample suction port 1111 is less than or equal to 200 μ L. The erythrocyte sedimentation rate detection pipe section 1131 is a part of the sample suction pipeline 113, and the erythrocyte sedimentation rate detection pipe section 1131 does not need to be arranged independently, so that the structure is simplified. The volume of the fluid channel between the sedimentation tube 1131 and the sample sucking port 1111 is the volume of the fluid channel from the end of the sedimentation tube 1131 close to the sampling needle 111 to the sample sucking port 1111, i.e. the volume of the sampling needle 111 is equal to the sum of the volume of the tube segment of the sample sucking line 113 between the sampling needle 111 and the sedimentation tube 1131. Before the sampling device 110 samples, the sample suction pipeline 113 and the sampling needle 111 are filled with the diluent 150, and the volume of a fluid channel between the blood sedimentation detection pipeline section 1131 and the sample suction port 1111 of the sampling needle 111 is designed to be less than or equal to 200 μ L in the embodiment, so that the distance between the blood sedimentation detection pipeline section 1131 and the sampling needle 111 can be ensured to be close enough, and the dilution degree of the diluent 150 remained in the sample suction pipeline 113 of the blood sample 160 to be detected and the amount of the blood sample 160 remained on the pipe wall can be reduced when the blood sample 160 to be detected is driven and conveyed to the blood sedimentation detection pipeline section 1131, thereby effectively ensuring the accuracy of blood sedimentation detection. In addition, in this embodiment, the blood sedimentation detecting assembly 141 and the sampling needle 111 are designed to be capable of moving relatively, so that the setting position of the blood sedimentation detecting assembly 141 can be flexible without being limited by the structure of the sampling device 110, and the blood sedimentation detecting assembly 141 only needs to be installed beside the non-moving part of the sample suction pipeline 113.
As an embodiment, the sedimentation detection assembly 141 includes a light source 1411 and a photoelectric converter 1412, and the light source 1411 and the photoelectric converter 1412 are respectively disposed on two sides of the sedimentation detection pipe section 1131 for implementing light absorption or light scattering measurement of the blood sample 160 to be detected in the sedimentation detection pipe section 1131. In a preferred embodiment, the light source 1411 and the photoelectric converter 1412 are disposed opposite to each other on both sides of the sedimentation tube section 1131.
As an embodiment, the sedimentation detection assembly 141 may also implement temperature control in addition to light absorption or light scattering measurements. Specifically, the erythrocyte sedimentation rate detecting assembly 141 further comprises a heater 1413 and a temperature sensor 1414, the heater 1413 is used for heating the blood sample 160 to be detected in the erythrocyte sedimentation rate detecting tube section 1131, and the temperature sensor 1414 is used for detecting and feeding back the temperature, so that the cooperation of the heater 1413 and the temperature sensor 1414 can realize the temperature control of the erythrocyte sedimentation rate detecting device 140 and the erythrocyte sedimentation rate detecting tube section 1131 penetrating through the erythrocyte sedimentation rate detecting device, the light source 1411 and the photoelectric converter 1412 can realize the light absorption or light scattering measurement of the blood sample 160 to be detected in the sample suction pipeline 113, and the whole ESR measurement process can be completed in one minute.
In one embodiment, the blood sedimentation detection apparatus 140 further comprises a fixing member 142, and the fixing member 142 is used for fixing the blood sedimentation detection pipe section 1131 and the blood sedimentation detection assembly 141. Specifically, the erythrocyte sedimentation rate detection tube 1131 passes through the erythrocyte sedimentation rate detection assembly 141 and is fixed by the fixing component 142, and in this embodiment, the erythrocyte sedimentation rate detection assembly 141 and the erythrocyte sedimentation rate detection tube 1131 are fixed by the same component, which is beneficial to simplifying the structure. Of course, in certain applications, the sedimentation test tubing segment 1131 and the sedimentation test assembly 141 may be separately positioned and mounted as an alternative embodiment.
As an embodiment, the fixing part 142 may be a fixing block, that is, the fixing part 142 may be a block-shaped part.
In one embodiment, the sample analyzer 100 further includes a body, the body is a main supporting structure of the sample analyzer 100, and the sampling device 110, the fluid path supporting device 120, the blood routine detecting device 130, and the blood sedimentation detecting device 140 are respectively fixed on the body.
In one embodiment, the motion driving device 112 and the fixing member 142 are independently mounted on the body, such that the fixing member 142 and the blood sedimentation detecting component 141 and the blood sedimentation detecting tube 1131 fixed thereon can be kept stationary with respect to the body, and the sampling needle 111 can move with respect to the fixing member 142, the blood sedimentation detecting component 141 and the blood sedimentation detecting tube 1131 under the driving of the motion driving device 112, which is very suitable for the application of the sampling device 110 with compact structure. Of course, in a specific application, the fixing member 142 is not limited to this, and as an alternative embodiment, the fixing member 142 may be mounted on a fixed portion of the motion driving device 112 that is stationary relative to the machine body, such as the transverse rail 1121 of the motion driving device 112.
As an embodiment, the sample analyzer 100 is configured to: before the sampling needle 111 collects a blood sample 160 to be tested, the sampling needle 111 and the sample suction pipeline 113 are filled with diluent 150; after the sampling needle 111 collects the blood sample 160 to be measured, an isolated gas column 170 is formed between the diluent 150 and the blood sample 160 to be measured. Specifically, after the sampling needle 111 collects the blood sample 160 to be tested, the blood sample 160 to be tested is filled in the sampling needle 111 or in the sampling needle 111 and the sample suction line 113; the isolating gas column 170 is filled between the blood sample 160 to be tested and the diluent 150 for isolating the blood sample 160 to be tested from the diluent 150, so that the blood sample 160 to be tested is not diluted. The isolated gas column 170 is formed by: the sampling needle 111 sucks a certain amount of gas before sucking the blood sample 160 to be measured, and after the sample is sucked, the gas sucked in front forms an isolation gas column 170 between the blood sample 160 to be measured and the diluent 150, and the gas forming the isolation gas column 170 may be air or other gas which is difficult to dissolve in the diluent 150 and the blood sample 160 to be measured.
In one embodiment, the volume of the fluid channel from the blood sedimentation tube 1131 to the sample suction port 1111 is greater than the preset channel volume, which is the sum of the preset maximum sample suction amount and the volume of the isolation gas column 170, and is less than or equal to 200 μ L, wherein the preset maximum sample suction amount is the maximum volume of the sample analyzer 100 that the sampling needle 111 is set by default to suck the blood sample at one time, and the volume of the blood sample actually collected at each time is less than or equal to the maximum sample suction amount. The volume of the fluid channel between the blood sedimentation detection tube section 1131 and the sample suction port 1111 is greater than the preset channel volume and less than or equal to 200 μ L, which not only can ensure that the distance between the sample suction pipeline 113 and the sampling needle 111 is close enough to reduce the dilution degree of the residual diluent 150 in the sample to be detected 160 by the sample suction pipeline 113 and reduce the amount of the residual diluent 160 in the tube wall, but also can prevent the heat of the heater 1413 from causing the isolation gas column 170 to be heated and expanded, thereby affecting the sample suction precision or the subsequent sample separation precision. Specifically, during the sample sucking and separating process of the sampling device 110, the diluent 150 and the shielding gas column 170 move with the blood sample 160 to be tested. The isolation gas column 170 separates the diluent 150 and the blood sample 160 to be tested, so as to prevent the diluent 150 and the blood sample 160 from directly mixing with each other.
In one embodiment, the liquid path supporting device 120 fills a part of the sample suction line 113 with the diluent 150 before the sample suction, and fills a part of the sample suction line 113 and the sampling needle 111 near the sampling needle 111 with gas, and after the sample suction, forms the isolation gas column 170 between the blood sample 160 to be measured and the diluent 150. In this embodiment, before the sample drawing, the diluent 150 is filled in a part of the sample drawing line 113, and the gas for forming the shielding gas column 170 is filled in the sampling needle 111 and another part of the sample drawing line 113. Of course, in a specific application, the filling positions of the diluent 150 and the gas before the sample drawing are not limited to this, for example, as an alternative embodiment, before the sample drawing, the liquid path supporting device 120 fills the diluent 150 in the sample drawing pipeline 113 and the gas in the sampling needle 111, and after the sample drawing, the isolation gas column 170 is formed between the blood sample 160 to be measured and the diluent 150, in this alternative embodiment, before the sample drawing, the sample drawing pipeline 113 is filled with the diluent 150, and the gas for forming the isolation gas column 170 fills the sampling needle 111; still alternatively, as another alternative embodiment, before the sample is drawn, the liquid path support module enables the sample drawing pipeline 113 and the sampling needle 111 to be filled with the diluent 150, the sampling needle 111 draws a preset volume or a certain amount of gas before drawing the blood sample 160 to be measured, and after the sample is drawn, the isolated gas column 170 is formed between the blood sample 160 to be measured and the diluent 150, in this alternative embodiment, the diluent 150 is filled in the sample drawing pipeline 113 and a part of the sampling needle 111, and the gas for forming the isolated gas column 170 is filled in another part of the sampling needle 111.
In one embodiment, the volume of the isolated gas column 170 is 5 μ L or more and 20 μ L or less. The isolating gas column 170 in this range can not only ensure the effect of isolating the blood sample 160 to be tested from the diluent 150, but also keep the sample suction pipeline 113 to have a proper length, thereby ensuring the rapid sample suction and separation.
As a preferred embodiment of this embodiment, the volume of the isolated gas column 170 is greater than or equal to 10 μ L and less than or equal to 15 μ L, and the effect of isolating the diluent 150 and the blood sample 160 is better in this range of isolated gas column 170.
In one embodiment, the sample line 113 includes a first sample line segment 1132, a sedimentation line segment 1131, and a second sample line segment 1133 serially connected in series between the sampling needle 111 and the fluid driving device 121. Specifically, one end of the first sampling tube 1132 is connected to the sampling needle 111, and the other end is connected to the blood sedimentation detection tube 1131 penetrating through the blood sedimentation detection device 140; one end of the second pipette tip 1133 is connected to the sedimentation tube 1131, and the other end is connected to the fluid circuit supporting apparatus 120. The sample suction pipeline 113 formed by the first sample suction pipe section 1132, the blood sedimentation detection pipe section 1131 and the second sample suction pipe section 1133 may be an integrally formed pipeline, or may be formed by connecting several pipelines in series in sequence.
As an embodiment, the sample analyzer 100 is configured to: after the sampling device 110 collects the blood sample 160 to be tested, the fluid driving device 121 is controlled to distribute a portion of the blood sample 160 to be tested in the sampling device 110 to the blood sedimentation testing device 130, and then the fluid driving device 121 is controlled to pump the remaining blood sample 160 to be tested in the sampling device 110 to the blood sedimentation testing tube 1131. This embodiment at first divides the appearance to the conventional detection device 130 of blood, divides the appearance to the sedimentation test tube section 1131 again, can avoid the blood sample 160 that awaits measuring that the blood conventional detected to receive the dilution influence of long distance pipeline, makes the branch appearance consuming time few simultaneously, guarantees measuring speed.
As a preferred embodiment of this embodiment, the operation of the sample analyzer 100 includes: before the measurement is prepared, the sample suction pipeline 113 and the sampling needle 111 are filled with diluent 150; before the sampling needle 111 sucks a sample, firstly sucking a section of air in a standby position to establish a section of isolation air column 170, and then moving to a sample sucking position to suck a blood sample 160 to be detected in a sample container 190; after the sample collection is completed, the blood is first dispensed to the blood routine testing device 130 through the sampling needle 111, and then the remaining blood sample 160 to be tested is drawn to the sedimentation testing tube section 1131. According to the embodiment, by optimizing the blood separation sequence between the blood routine detecting device 130 and the blood sedimentation detecting device 140, arranging the isolation air column 170 between the blood sample 160 to be detected and the diluent 150, and optimizing and limiting the volume of the fluid channel between the blood sedimentation detecting component 141 and the sample suction port 1111 of the sampling needle 111, the efficiency of the blood routine detection and the blood sedimentation detection can be guaranteed, and the technical problem of low accuracy of the blood sedimentation detection result caused by dilution of the blood sample 160 to be detected of the blood sedimentation detecting device 140 can be effectively solved.
Example two:
as shown in fig. 2, 6 and 7, the sample analyzer 100 provided in this embodiment is different from the first embodiment mainly in that: in this embodiment, an isolated blood sample 180 is further provided on the basis of the first embodiment, and the number of the isolated gas columns 170 is two or more.
Specifically, in the present embodiment, the sample analyzer 100 is configured to: after the sampling needle 111 collects the blood sample 160 to be measured, more than two isolated gas columns 170 are formed between the diluent 150 and the blood sample 160 to be measured at intervals, and one isolated blood sample 180 is formed between any two adjacent isolated gas columns 170. The isolated gas column 170 can prevent the diluent 150 and the isolated blood sample 180 or the isolated blood sample 180 and the blood sample 160 to be tested from directly mixing with each other. The setting of isolation blood sample 180 can further reduce the dilution influence and the loss volume when the blood sample 160 that awaits measuring drags for a long distance, guarantees the accuracy that the blood sedimentation detected under the far away circumstances of sampling needle 111 at blood sedimentation detection subassembly 141.
As an embodiment, the sample analyzer 100 is configured to: after the sampling needle 111 collects the blood sample 160 to be measured, a first isolated gas column 171, an isolated blood sample 180, and a second isolated gas column 172 are sequentially formed between the diluent 150 and the blood sample 160 to be measured. A section of isolated blood sample 180 is arranged between the diluent 150 and the blood sample 160 to be detected, the diluent 150 and the isolated blood sample 180 are separated by a first isolated gas column 171, the isolated blood sample 180 and the blood sample 160 to be detected are separated by a second isolated gas column 172, and the diluent 150 and the isolated blood sample 180 or any two adjacent sections of liquid of the isolated blood sample 180 and the blood sample 160 to be detected are prevented from being directly mixed with each other. In the sample analyzer 100 provided in this embodiment, two isolated gas columns 170 and one isolated blood sample 180 are formed between the diluent 150 and the blood sample 160 to be tested, but, in a specific application, the number of the isolated gas columns 170 and the isolated blood sample 180 is not limited thereto, for example, as an alternative embodiment, three isolated gas columns 170 and two isolated blood samples 180 are formed between the diluent 150 and the blood sample 160 to be tested; alternatively, as another alternative embodiment, four isolated gas columns 170 and three isolated blood samples 180 are formed between the diluent 150 and the blood sample 160 to be tested.
In one embodiment, the isolated blood sample 180 has a volume of 5 μ L or more. Here, isolating the volume of blood sample 180 specifically refers to isolating the initial volume of blood sample 180, i.e., isolating the volume of blood sample 180 after sampling is complete and before aliquoting. Here, through optimizing the volume of keeping apart blood specimen 180 and setting up, can guarantee to await measuring blood specimen 160 and drag to the blood sedimentation test tube section 1131 time, keep apart the initial volume that blood specimen 180 remained the quantity less than or equal to of pipe wall at the isolation blood specimen 180 to guarantee to keep apart the setting effect of blood specimen 180, thereby effectively reduce the dilution degree of blood specimen 160 that awaits measuring and the volume of blood specimen 160 residual loss on the pipe wall that awaits measuring, ensured the accuracy that the blood sedimentation detected.
In one embodiment, in order to ensure the accuracy of the sample suction and dispensing, the air in the sample suction line 113 needs to be minimized, and the volume of the two isolation air columns 170 is 5 μ L or more and 20 μ L or less.
As a preferred embodiment of this embodiment, the operation of the sample analyzer 100 includes: before the measurement is prepared, the sample suction pipeline 113 and the sampling needle 111 are filled with diluent 150; before the sampling needle 111 sucks a sample, firstly sucking a section of air at a standby position to establish a first isolated air column 171, then moving to a sample sucking position to suck a section of blood sample in a sample container 190 to establish an isolated blood sample 180; then returning to the standby position, sucking a section of air to establish a second isolated air column 172, and finally moving to the sample sucking position to suck a section of blood sample in the sample container 190 to establish a blood sample 160 to be tested; after the sample suction is completed, the blood is first fractionated by the sampling needle 111 to the blood routine test device 130, and then the remaining blood sample 160 to be tested is pulled to the blood sedimentation test tube section 1131. The first isolation gas column 171 separates the diluent 150 from the isolated blood sample 180, and the second isolation gas column 172 separates the isolated blood sample 180 from the blood sample 160 to be tested, thereby preventing direct mixing between the two. This embodiment, at first divide the blood to conventional detection device 130 of blood and can avoid the blood sample 160 that awaits measuring that the blood conventional testing detected to receive the dilution influence of long distance pipeline, make simultaneously that it consumes less to divide the blood, guarantee measuring speed.
When the blood sample 160 to be tested is dragged to the blood sedimentation tube 1131 for a long distance, a small amount of the diluent 150 will remain on the inner wall of the sample suction line 113 and mix into the isolated blood sample 180 due to the wetting characteristics of the diluent 150 and the inner wall of the sample suction line 113. The diluent 150 mixed into the isolated blood sample 180 is mixed with the isolated blood sample 180, and then remains on the inner wall of the sample suction line 113 again, and is mixed into the blood sample 160 to be measured. Defining the volume of the isolated blood sample 180 as V before the blood sample 160 to be tested is directed to the blood sedimentation measurement tube section 1131 S Defining the volume of the blood sample 160 to be tested as V before the blood sample 160 to be tested is sent to the blood sedimentation tube section 1131 T The volume of the diluent 150 remaining on the inner wall of the sample suction line 113 per unit distance advanced in the sample suction line 113 is defined as V 1 Defining the unit distance of blood sample per advance in the sample suction line 113The residual volume of the inner wall of the passage 113 is V 2 In which V is 1 ≈V 2 <<V S . Assuming that the diluent 150 is mixed into the isolated blood sample 180 and is sufficiently mixed, and that the change in the total volume of the isolated blood sample 180 and the blood sample 160 to be tested due to the residue is ignored, the volume of the diluent 150 mixed into the blood sample 160 to be tested is V (V) per unit distance of advance D1 Comprises the following steps:
V D1 ＝V 1 *V 2 /(V S +V 1 -V 2 )≈V 1 *V 2 /V S ；
in the case where only one isolation gas column 170 separates the diluent 150 from the blood sample 160, the diluent 150 mixed in the blood sample 160 has a volume V D2 Comprises the following steps:
V D2 ≈V 1 ；
due to V 1 ≈V 2 <<V S Therefore V is D1 <<V D2 。
It can be seen that the total amount of the diluent 150 finally mixed into the blood sample 160 to be tested is much smaller than the total residual amount of the diluent 150 on the inner wall of the sample suction pipeline 113 due to the isolated blood sample 180.
In addition to the above differences, other parts of the sample analyzer 100 provided in this embodiment can be optimally designed with reference to the first embodiment, and will not be described in detail herein.
Example three:
as shown in fig. 2, 6 and 8 to 11, the sample analyzer 100 provided in the present embodiment is different from the first embodiment mainly in that: the technical means adopted for solving the technical problem that the blood sample 160 to be measured is easily diluted by the diluent 150 or has a large loss in the sample suction pipeline 113 and the volume of the fluid channel between the blood sedimentation detection pipe section 1131 and the sample suction port 1111 of the sampling needle 111 are different. Specifically, in the first embodiment, the volume of the fluid channel between the sedimentation detection pipe section 1131 and the sample sucking port 1111 of the sampling needle 111 is mainly limited, and the isolation air column 170 is established, so as to reduce the dilution degree of the blood sample 160 to be measured by the diluent 150 and reduce the loss of the blood sample 160 to be measured in the sample sucking pipeline 113, which is mainly suitable for the sample analyzer 100 with the small volume of the fluid channel between the sedimentation detection pipe section 1131 and the sample sucking port 1111 of the sampling needle 111; in the embodiment, the isolated blood sample 180 and the two or more isolated gas columns 170 are mainly established to reduce the dilution degree of the blood sample 160 to be measured by the diluent 150 and reduce the loss of the blood sample 160 to be measured in the sample suction pipeline 113, and the isolated gas column is mainly suitable for the sample analyzer 100 with a large fluid channel volume between the blood sedimentation detection pipe section 1131 and the sample suction port 1111 of the sampling needle 111.
Specifically, as in the first embodiment, the sample analyzer 100 provided in this embodiment also includes a sampling device 110, a fluid circuit supporting device 120, a blood routine detecting device 130, and a blood sedimentation detecting device 140, where the sampling device 110 also includes a sampling needle 111, a sample suction pipeline 113 connected to the sampling needle 111, and a motion driving device 112 for driving the sampling needle 111 to move, and the sampling needle 111 also has a sample suction port 1111 far from the sample suction pipeline 113; the liquid path supporting device 120 also comprises a fluid driving device 121 which is communicated with the sample suction pipeline 113 and is used for providing driving force for the fluid to flow in the sampling needle 111 and the sample suction pipeline 113; the blood routine detecting device 130 also comprises a detecting pool 131 and a blood routine detecting component, the blood sedimentation detecting device 140 also comprises a blood sedimentation detecting component 141, a part of the sample suction pipeline 113 is also used as a blood sedimentation detecting pipeline section 1131, and the sampling needle 111 can also move relative to the blood sedimentation detecting component 141 under the driving of the motion driving device 112; the sample analyzer 100 is also configured to: before the sampling needle 111 collects the blood sample 160 to be measured, the sampling needle 111 and the sample suction line 113 are filled with the diluent 150.
Unlike the first embodiment, the present embodiment provides the sample analyzer 100 further configured to: after the sampling needle 111 collects the blood sample 160 to be tested, more than two segments of isolation gas columns 170 which are arranged at intervals are formed between the diluent 150 and the blood sample 160 to be tested, and a segment of isolation gas column 180 is formed between any two adjacent segments of isolation gas columns 170. The principle of isolating the blood sample 180 to reduce the dilution and loss of the blood sample 160 to be tested can be found in the second embodiment, and will not be described in detail herein.
As an embodiment, the sample analyzer 100 is configured to: after the sampling needle 111 collects the blood sample 160 to be measured, a first isolated gas column 171, an isolated blood sample 180, and a second isolated gas column 172 are sequentially formed between the diluent 150 and the blood sample 160 to be measured. A section of isolated blood sample 180 is arranged between the diluent 150 and the blood sample 160 to be tested, the diluent 150 and the isolated blood sample 180 are separated by a first isolated gas column 171, and the isolated blood sample 180 and the blood sample 160 to be tested are separated by a second isolated gas column 172. In the sample analyzer 100 provided in this embodiment, two isolated gas columns 170 and one isolated blood sample 180 are formed between the diluent 150 and the blood sample 160 to be tested, but the number of the isolated gas columns 170 and the isolated blood sample 180 is not limited to this, for example, as an alternative embodiment, three isolated gas columns 170 and two isolated blood samples 180 are formed between the diluent 150 and the blood sample 160 to be tested; alternatively, as another alternative embodiment, four isolated gas columns 170 and three isolated blood samples 180 are formed between the diluent 150 and the blood sample 160 to be tested.
In one embodiment, the volume of the fluid channel between the sedimentation measurement tubing section 1131 and the sample suction port 1111 is equal to or greater than 100 μ L and equal to or less than 800 μ L. The blood sample 160 to be tested of the first embodiment is diluted to a lesser extent when being delivered at a short distance; by the arrangement of the isolated blood sample 180, the blood sample 160 to be tested can be diluted to a smaller extent when being transported over a long distance. In this embodiment, the volume of the fluid channel between the blood sedimentation detection tube section 1131 and the sample suction port 1111 is set within a range of 100 μ L or more and 800 μ L or less, which on one hand can facilitate ensuring that the blood sedimentation detection device 140 can be installed outside the motion assembly of the sampling device 110, so that the installation of the blood sedimentation detection device 140 is not affected by the structure of the sampling device 110; on the other hand, the adverse phenomenon that the spatial movement of the sampling needle 111 interferes with the blood sedimentation detection assembly 141 due to the too close distance between the blood sedimentation detection pipe section 1131 and the sampling needle 111 can be avoided, and the adverse phenomenon that the heat of the heater 1413 causes the isolation air column 170 to be heated and expanded can be avoided; on the other hand, the adverse phenomenon that the residual loss and dilution of the blood sample 160 to be detected in the sample suction pipeline 113 are greatly influenced due to the overlong distance between the blood sedimentation detection pipeline section 1131 and the sample suction port 1111 can be avoided, and the adverse phenomenon that the time required for dragging the blood sample 160 to be detected to the blood sedimentation detection pipeline section 1131 is overlong is favorably avoided, so that the efficiency of blood sedimentation detection is favorably ensured.
As a preferred embodiment of this embodiment, the blood sedimentation detecting component 141 is installed outside the sampling device 110 and beside the portion of the sample suction line 113 far from the sampling needle 111, i.e. the blood sedimentation detecting component 141 is disposed near the fluid circuit supporting device 120. With such an installation manner, the technical problem that in some sample analyzers 100 (for example, small sample analyzers 100 using the swing-arm type sampling device 110), the holder of the sampling needle 111 is very compact in volume and is difficult to accommodate the blood sedimentation detection device 140 can be solved. Although the blood sedimentation detecting component 141 is installed outside the sampling device 110 and far away from the side of the partial sample suction pipeline 113 of the sampling needle 111, the pipeline through which the blood sample 160 to be detected is conveyed from the sampling needle 111 to the blood sedimentation detecting device 140 needs to flow is long, the existence of the isolated blood sample 180 can ensure that the total amount of the diluent 150 finally mixed into the blood sample 160 to be detected is far smaller than the total residual amount of the diluent 150 on the inner wall of the sample suction pipeline 113, and the total volume change of the blood sample 160 to be detected caused by the residual can be ensured, so that the dilution influence of the residual liquid on the tube wall on the blood sample 160 to be detected is very small, and the accuracy of the blood sedimentation detection is effectively ensured. As shown in fig. 8 and 9, the fluid driving device 121 may further include an injector 1211, the volume of the injector 1211 is 500 μ L or less, the fluid path supporting device 120 may further include a fluid discharge line 122 and a first line switching unit 123, and the injector 1211 may switchably connect the sample suction line 113 and the fluid discharge line 122 through the first line switching unit 123. In this embodiment, the small volume of the syringe 1211 used for aspirating and dispensing the blood sample is advantageous for ensuring the accuracy of dispensing the blood sample to the blood routine testing device 130. Because of the small volume of the injector 1211, the blood sample 160 to be tested cannot be directly drawn to the sedimentation detection pipe section 1131, and in the present embodiment, the small volume of the injector 1211 is matched with the first pipeline switching component 123, so that the distance of drawing the blood sample 160 to be tested can exceed the maximum volume stroke of the injector 1211, thereby avoiding the problems of cost increase and volume increase caused by adding a large volume of the injector 1211 additionally. When the distance from the blood sedimentation tube section 1131 to the sampling needle 111 exceeds the volume stroke of the injector 1211, the injector 1211 can be used to draw the blood sample 160 to be tested to the blood sedimentation tube section 1131 through the first pipeline switching component 123 for a plurality of times of sample suction and liquid discharge. Specifically, when the blood sample 160 to be tested starts to be drawn to the sedimentation detection pipe section 1131, the first pipeline switching component 123 connects the injector 1211 with the sample suction pipeline 113, and the injector 1211 sucks liquid from the sample suction pipeline 113 to draw the blood sample 160 to be tested towards the injector 1211; when the liquid suction amount of the syringe 1211 reaches the maximum volume stroke, the first tube switching means 123 switches the state, the syringe 1211 discharges the liquid therein to the liquid discharge tube 122 and to the other part of the liquid path supporting means 120, and then the syringe 1211 and the sample suction tube 113 are again communicated, and the blood sample 160 to be measured is drawn toward the syringe 1211; this is repeated until the blood sample 160 is drawn to the blood sedimentation tube 1131.
In one embodiment, the first channel switching unit 123 includes a first three-way valve 1231, and three ports of the first three-way valve 1231 communicate with the syringe 1211, the sample suction channel 113, and the liquid discharge channel 122, respectively. When the blood sample 160 to be tested is dragged to the blood sedimentation detection pipe section 1131, the first three-way valve 1231 is communicated with the injector 1211 and the sample suction pipeline 113, the passage between the first three-way valve 1231 and the liquid discharge pipeline 122 is closed, the injector 1211 sucks liquid from the sample suction pipeline 113, and the blood sample 160 to be tested is dragged towards the injector 1211; when the liquid absorption amount of the injector 1211 reaches the maximum volume stroke, the first three-way valve 1231 is switched to a state, at this time, the first three-way valve 1231 is communicated with the injector 1211 and the liquid discharge pipeline 122, the passage between the first three-way valve 1231 and the sample absorption pipeline 113 is closed, the injector 1211 discharges the liquid in the injector 1211 to the liquid discharge pipeline 122, and then the first three-way valve 1231 is switched to a state again to communicate the injector 1211 and the sample absorption pipeline 113, so that the blood sample 160 to be detected is dragged towards the injector 1211; this is repeated until the blood sample 160 to be tested is drawn to the blood sedimentation device 140. Of course, in a specific application, the first pipeline switching component 123 is not limited to use a three-way valve, for example, as an alternative embodiment, the first pipeline switching component 123 may also use a combination of two on-off valves and a three-way joint to replace the three-way valve, specifically, in the alternative embodiment, the first pipeline switching component 123 includes a first on-off valve, a second on-off valve and a first three-way joint, three ports of the first three-way joint are respectively communicated with the injector 1211, the sample suction pipeline 113 and the liquid discharge pipeline 122, the first on-off valve is disposed on the sample suction pipeline 113, the second on-off valve is disposed on the liquid discharge pipeline 122, when the blood sample 160 to be tested starts to be dragged to the blood sedimentation detection pipe section 1131, the first on-off valve is opened, the second on-off valve is closed, the injector 1211 sucks liquid from the sample suction pipeline 113, and drags the blood sample 160 to be tested to the injector 1211; when the liquid absorption amount of the injector 1211 reaches the maximum volume stroke, the first switch valve is closed, the second switch valve is opened, the injector 1211 is communicated with the liquid discharge pipeline 122 to discharge the liquid in the injector 1211, then the first switch valve is opened, the second switch valve is closed, and the blood sample 160 to be tested is dragged towards the injector 1211; this is repeated until the blood sample 160 is drawn to the blood sedimentation device 140.
In the preferred embodiment of the present embodiment, by optimizing the sample separation sequence between the blood routine detecting device 130 and the blood sedimentation detecting device 140 and arranging the dual isolated gas columns 170 and the isolated blood samples 180 between the blood sample 160 to be detected and the diluent 150, the dilution problem of the blood sample 160 to be detected and the residual loss problem of the blood sample 160 to be detected on the tube wall due to the excessively long distance between the blood sedimentation detecting device 140 and the sampling needle 111 can be effectively solved. In addition, the sample suction syringe 1211 with small volume is matched with the first pipeline switching part 123, so that the dragging distance of the sample 160 to be tested can exceed the maximum volume stroke of the syringe 1211, and the problems of high cost and large volume caused by additionally adding a large-volume syringe 1211 are solved.
In addition to the above differences, the present embodiment provides other parts of the sample analyzer 100 with reference to the first embodiment or the second embodiment, which are not described in detail herein.
Example four:
as shown in fig. 2, fig. 8 and fig. 12, the sample analyzer 100 of the present embodiment differs from the first to third embodiments mainly in the connection manner of the sample suction line 113, the fluid circuit supporting device 120 and the blood sedimentation detecting device 140.
Specifically, in this embodiment, the sample suction pipeline 113 includes a main branch path 1134, a first branch path 1135, and a second branch path 1136, the fluid driving device 121 includes a first power device 1212 and a second power device 1213 that are independent of each other, one end of the main branch path 1134 is connected to the sampling needle 111, one end of the first branch path 1135 and one end of the second branch path 1136 intersect and are connected to the other end of the main branch path 1134, the other end of the first branch path 1135 is connected to the first power device 1212, the other end of the second branch path 1136 is connected to the second power device 1213, and a part of the first branch path 1135 forms a blood sedimentation detection pipe segment 1131; first branch road 1135 mainly used realizes the erythrocyte sedimentation rate and detects the function, and second branch road 1136 is used for realizing drawing appearance and the conventional branch appearance function of blood, and with the erythrocyte sedimentation rate detect function and draw appearance and the conventional branch appearance function of blood respectively through two different branches of drawing appearance pipeline 113, do benefit to erythrocyte sedimentation rate and detect and the conventional detection of blood can go on simultaneously to save measuring time, improved measuring speed.
Specifically, the first power device 1212 and the second power device 1213 are two power sources that are independent of each other, such as two injectors that are independent of each other, or other pressure sources that can provide positive and negative power.
The sampling device 110 further includes a second circuit switching part 114, the second circuit switching part 114 is disposed on the first branch 1135 and is located between the erythrocyte sedimentation rate detection pipe section 1131 and the main branch 1134, and the erythrocyte sedimentation rate detection pipe section 1131 switchably connects the main branch 1134 and the outside air through the second circuit switching part 114. The second circuit switching component 114 can be switched to the arrangement mode communicated with the outside air, which is mainly used to ensure that when the second power device 1213 distributes the blood sample 160 left in the main branch 1134 and the sampling needle 111 to the routine blood testing device 130 through the second branch 1136, the first power device 1212 can drag the blood sample 160 in the first branch 1135 to the sedimentation testing tube section 1131.
As an embodiment, the sample analyzer 100 is configured to: after the sampling device 110 collects the blood sample 160 to be tested, the first power device 1212 and the fluid driving device 121 are controlled to pump part of the blood sample 160 to be tested in the sampling device 110 to the first branch 1135, and then the second power device 1213 and the fluid driving device 121 are controlled to distribute the remaining blood sample 160 to be tested in the sampling device 110 to the routine blood testing device 130, and at the same time, the first power device 1212 and the blood sample 160 to be tested in the first branch 1135 are controlled to be pumped to the sedimentation testing pipe section 1131. In specific application, when sample separation starts, the second pipeline switching component 114 is switched to a state of communicating the erythrocyte sedimentation detection pipe section 1131 with the main branch pipeline 1134, and the first power device 1212 sucks a part of the blood sample 160 to be detected for erythrocyte sedimentation detection into the first branch pipeline 1135 and between the second pipeline switching component 114 and the erythrocyte sedimentation detection pipe section 1131; then, the second pipeline switching part 114 is switched to a state of communicating the sedimentation detection pipe section 1131 with the outside air; the second power device 1213 distributes the blood sample 160 remaining in the main branch 1134 and the sampling needle 111 to the blood routine testing device 130 through the second branch 1136 for blood routine testing, and the first power device 1212 continues to drag the blood sample 160 to be tested in the first branch 1135 to the blood sedimentation testing tube 1131 for blood sedimentation testing. In this way, the blood sedimentation test and the routine blood test can be performed simultaneously, thereby saving measurement time.
As an embodiment, the second pipeline switching component 114 includes a second three-way valve, three ports of the second three-way valve are respectively communicated with the sedimentation detection pipe section 1131, the main branch 1134 and the outside air; when the sample separation starts, the sedimentation detection pipe section 1131 is communicated with the main branch 1134 through a second three-way valve, and the first power device 1212 sucks the blood sample 160 to be detected into the first branch 1135; then the second three-way valve disconnects the first branch 1135 from the main branch 1134 and connects with the outside air, and the second power device 1213 distributes the blood sample 160 to be measured in the sample suction line 113 and the sampling needle 111 to the blood sedimentation detection device 130 through the second branch 1136, and simultaneously the first power device 1212 continues to drag the blood sample 160 to be measured in the first branch 1135 to the blood sedimentation detection device 140 for ESR measurement. Of course, in a specific application, the first pipeline switching part 123 is not limited to a three-way valve, for example, as an alternative embodiment, two on-off valves and a three-way joint may be used instead of the three-way valve. Specifically, the second pipeline switching component 114 includes a third on-off valve, a fourth on-off valve, and a second three-way joint, the second three-way joint is disposed on the first branch 1135, three ports of the second three-way joint are respectively communicated with the sedimentation detection pipe section 1131, the third on-off valve, and the fourth on-off valve, the third on-off valve is disposed between the main branch 1134 and the second three-way joint, and the fourth on-off valve is disposed between the outside air and the second three-way joint. When the sample separation starts, the third switch valve is opened, the fourth switch valve is closed, the blood sedimentation detection pipe section 1131 is communicated with the main branch 1134, the blood sedimentation detection pipe is not communicated with the outside air, and the first power device 1212 sucks the blood sample 160 to be detected into the first branch 1135; then, the third switch valve is closed, the fourth switch valve is opened, the sedimentation detection pipe section 1131 is disconnected from the main branch 1134 and is connected to the outside air, the second power device 1213 distributes the blood sample 160 to be measured in the sample suction line 113 and the sampling needle 111 to the routine blood detection device 130 through the second branch 1136, and simultaneously the first power device 1212 continues to drag the blood sample 160 to be measured in the first branch 1135 to the sedimentation detection device 140 for ESR measurement.
In addition to the above differences, the present embodiment provides other parts of the sample analyzer 100 that can be optimally designed with reference to any one of the first to third embodiments, and will not be described in detail herein.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (18)
1. A sample analyzer, comprising: the method comprises the following steps:
the sampling device comprises a sampling needle, a sample suction pipeline connected with the sampling needle and a motion driving device for driving the sampling needle to move, wherein the sampling needle is provided with a sample suction port far away from the sample suction pipeline;
the liquid path supporting device comprises a fluid driving device which is communicated with the sample sucking pipeline and is used for providing driving force for the fluid to flow in the sampling needle and the sample sucking pipeline;
the blood routine detection device comprises a detection pool and a blood routine detection assembly, the detection pool is used for providing a blood routine detection place for the blood sample to be detected distributed by the sampling device, and the blood routine detection assembly is used for performing blood routine detection on the blood sample to be detected in the detection pool;
the blood sedimentation detection device comprises a blood sedimentation detection assembly, one part of the sample suction pipeline is used as a blood sedimentation detection pipeline section, the blood sedimentation detection pipeline section is used for providing a blood sedimentation detection place for a blood sample to be detected, and the blood sedimentation detection assembly is used for performing blood sedimentation detection on the blood sample to be detected in the blood sedimentation detection pipeline section;
the sampling needle can move relative to the blood sedimentation detection assembly under the driving of the movement driving device, and the volume of a fluid channel between the blood sedimentation detection pipe section and the sample suction port is less than or equal to 200 mu L.
2. The sample analyzer of claim 1 wherein: the sample analyzer is configured to: before the sampling needle collects the blood sample to be detected, diluent is filled in the sampling needle and the sample suction pipeline;
after the sampling needle collects the blood sample to be detected, an isolation gas column is formed between the diluent and the blood sample to be detected, the volume of a fluid channel between the blood sedimentation detection pipe section and the sample suction port is larger than the volume of a preset channel and is less than or equal to 200 mu L, and the volume of the preset channel is the sum of the volume of a preset maximum sample suction amount and the volume of the isolation gas column.
3. The sample analyzer of claim 2 wherein: the sample analyzer is configured to: the sampling needle gather the blood sample that awaits measuring after the diluent with it sets up to await measuring to be formed with the interval more than two sections between the blood sample keep apart the gas column, and arbitrary adjacent two sections be formed with one section between the gas column keeps apart the blood sample.
4. The sample analyzer of claim 3, wherein: the sample analyzer is configured to: after the sampling needle collects the blood sample to be detected, a first isolation gas column, the isolation blood sample and a second isolation gas column are sequentially formed between the diluent and the blood sample to be detected.
5. The sample analyzer of any of claims 2 to 4, wherein: the volume of the isolated gas column is more than or equal to 5 mu L and less than or equal to 20 mu L.
6. The sample analyzer of claim 3 or 4, wherein: the volume of the isolated blood sample is greater than or equal to 5 μ L.
7. A sample analyzer, comprising: the method comprises the following steps:
the sampling device comprises a sampling needle, a sample sucking pipeline connected with the sampling needle and a motion driving device used for driving the sampling needle to move, wherein the sampling needle is provided with a sample sucking port far away from the sample sucking pipeline;
the liquid path supporting device comprises a fluid driving device which is communicated with the sample sucking pipeline and is used for providing driving force for the fluid to flow in the sampling needle and the sample sucking pipeline;
the blood routine detection device comprises a detection pool and a blood routine detection assembly, the detection pool is used for providing a blood routine detection place for the blood sample to be detected distributed by the sampling device, and the blood routine detection assembly is used for performing blood routine detection on the blood sample to be detected in the detection pool;
the blood sedimentation detection device comprises a blood sedimentation detection assembly, one part of the sample suction pipeline is used as a blood sedimentation detection pipeline section, the blood sedimentation detection pipeline section is used for providing a blood sedimentation detection place for a blood sample to be detected, and the blood sedimentation detection assembly is used for performing blood sedimentation detection on the blood sample to be detected in the blood sedimentation detection pipeline section;
the sampling needle can move relative to the blood sedimentation detection assembly under the driving of the movement driving device;
the sample analyzer is configured to: before the sampling needle collects the blood sample to be detected, diluent is filled in the sampling needle and the sample suction pipeline; after the sampling needle collects the blood sample to be detected, more than two sections of isolation gas columns which are arranged at intervals are formed between the diluent and the blood sample to be detected, and one section of isolation gas column is formed between any two adjacent sections of isolation gas columns.
8. The sample analyzer of claim 7, wherein: the sample analyzer is configured to: after the sampling needle collects the blood sample to be detected, a first isolation gas column, the isolation blood sample and a second isolation gas column are sequentially formed between the diluent and the blood sample to be detected.
9. The sample analyzer of claim 7 or 8, wherein: the volume of the isolated gas column is more than or equal to 5 mu L and less than or equal to 20 mu L; and/or the volume of the isolated blood sample is greater than or equal to 5 mu L.
10. The sample analyzer of claim 7 or 8, wherein: the volume of a fluid channel between the sedimentation detection pipe section and the sample suction port is more than or equal to 100 mu L and less than or equal to 800 mu L.
11. The sample analyzer of any of claims 1 to 4 or 7 or 8 wherein: the fluid driving device comprises an injector, the volume of the injector is less than or equal to 500 mu L, the liquid path supporting device also comprises a liquid discharge pipeline and a first pipeline switching part, and the injector is connected with the sample suction pipeline and the liquid discharge pipeline in a switchable manner through the first pipeline switching part.
12. The sample analyzer of claim 11, wherein: the first pipeline switching component comprises a first three-way valve, and three interfaces of the first three-way valve are respectively communicated with the injector, the sample sucking pipeline and the liquid discharging pipeline; alternatively, the first and second electrodes may be,
the first pipeline switching component comprises a first switch valve, a second switch valve and a first three-way joint, three interfaces of the first three-way joint are respectively communicated with the injector, the sample suction pipeline and the liquid discharge pipeline, the first switch valve is arranged on the sample suction pipeline, and the second switch valve is arranged on the liquid discharge pipeline.
13. The sample analyzer of any of claims 1 to 4 or 7 or 8 wherein: the sample suction pipeline comprises a first sample suction pipe section, a blood sedimentation detection pipe section and a second sample suction pipe section which are sequentially connected in series between the sampling needle and the fluid driving device.
14. The sample analyzer of claim 13 wherein: the sample analyzer is configured to: after the sampling device collects the blood sample to be detected, the fluid driving device is controlled to distribute part of the blood sample to be detected in the sampling device to the blood routine detection device, and then the fluid driving device is controlled to suck the rest of the blood sample to be detected in the sampling device to the blood sedimentation detection pipe section.
15. The sample analyzer of any of claims 1 to 4 or 7 or 8 wherein: the sample sucking pipeline comprises a main branch, a first branch and a second branch, the fluid driving device comprises a first power device and a second power device which are mutually independent, one end of the main branch is connected with the sampling needle, one end of the first branch and one end of the second branch are connected to the other end of the main branch in a crossed manner, the other end of the first branch is connected to the first power device, the other end of the second branch is connected to the second power device, and a part of the first branch forms the blood sedimentation detection pipe section;
the sampling device further comprises a second pipeline switching part, the second pipeline switching part is arranged on the first branch and located between the sedimentation detection pipe section and the main branch, and the sedimentation detection pipe section is connected with the main branch and the outside air in a switchable manner through the second pipeline switching part.
16. The sample analyzer of claim 15 wherein: the second pipeline switching part comprises a second three-way valve, and three interfaces of the second three-way valve are respectively communicated with the sedimentation detection pipe section, the main branch and the outside air; alternatively, the first and second electrodes may be,
the second pipeline switches part includes third ooff valve, fourth ooff valve and second three way connection, the second three way connection locates on the first branch road, just second three way connection's three interface communicates respectively the blood sedimentation detects the pipeline section third ooff valve with the fourth ooff valve, the third ooff valve is located main branch road with between the second three way connection, just the fourth ooff valve is located outside air with between the second three way connection.
17. The sample analyzer of claim 15 wherein: the sample analyzer is configured to: after the sampling device collects the blood sample to be detected, the first power device is controlled to suck part of the blood sample to be detected in the sampling device to the first branch path, then the second power device is controlled to distribute the rest blood sample to be detected in the sampling device to the conventional blood detection device, and meanwhile, the first power device is controlled to suck the blood sample to be detected in the first branch path to the blood sedimentation detection pipe section.
18. The sample analyzer of any of claims 1 to 4 or 7 or 8 wherein: the sample analyzer further comprises a machine body, the blood sedimentation detection device further comprises a fixing part for fixing the blood sedimentation detection pipe section and the blood sedimentation detection assembly, and the motion driving device and the fixing part are respectively and independently installed on the machine body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110807494.4A CN115615880A (en) | 2021-07-16 | 2021-07-16 | Sample analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110807494.4A CN115615880A (en) | 2021-07-16 | 2021-07-16 | Sample analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115615880A true CN115615880A (en) | 2023-01-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110807494.4A Pending CN115615880A (en) | 2021-07-16 | 2021-07-16 | Sample analyzer |
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| Country | Link |
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| CN (1) | CN115615880A (en) |
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2021
- 2021-07-16 CN CN202110807494.4A patent/CN115615880A/en active Pending
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