WO2019206276A1

WO2019206276A1 - Apparatus for use in analysis and detection and cell or particle detection method

Info

Publication number: WO2019206276A1
Application number: PCT/CN2019/084534
Authority: WO
Inventors: 罗进才; 叶长钦
Original assignee: 东莞德益生物医疗科技有限公司
Priority date: 2018-04-28
Filing date: 2019-04-26
Publication date: 2019-10-31

Abstract

An apparatus for use in analysis and detection and a cell or particle detection method. The apparatus for use in analysis and detection comprises a sample guiding tank body (21), capillary tubes (13), and collecting parts (16). The sample guiding tank body (21) comprises several laterally enclosing panels (11) sequentially connected head-to-tail and a bottom panel (19) in a tilted arrangement. The lower ends of the laterally enclosing panels (11) are connected to the bottom panel (19). The capillary tubes (13) are connected to the lower end of the base panel (19). The capillary tubes (13) and the collecting parts (16) are arranged from the high-position end to the low-position end in the direction of tilt of the base panel (19). The base panel (19) is provided with first sample inlets (12) running through the base panel (19). Liquid inlets of the capillary tubes (13) are in communication with the first sample inlets (12). The apparatus for analysis and detection is applicable in a quantitative detection and analysis of cells or particles and has the advantages of being structurally simple, convenient to operate, and capable of implementing a structural simplification of an operating instrument.

Description

Analytical detection device and cell or particle detection method

Technical field

The invention relates to the field of analytical detection, in particular to an analytical detection device and a cell or particle detection method.

Background technique

With the continuous advancement of science and technology, the detection and analysis equipment has developed in two directions: one is toward a more "high, precise, integrated" direction, which relies on large-scale automatic equipment and has batch detection. Fast speed, accurate results, etc., but there are also defects such as high instrument price, complicated operation, long time for single sample report, and the other is toward “simple, convenient and fast”, which is characterized by miniaturization of instruments. It is easy to operate and quick to report. Although its batch processing ability is not as good as that of large-scale automatic instruments, it has low instrument cost, simple sample processing, and is suitable for rapid detection on site.

In the fields of disease diagnosis, environmental monitoring, food and drug safety, it is very important to conduct on-site rapid quantitative analysis of cells and small particulate matter. In this field, the traditional method is to count the number of cells under the microscope using a Neubauer counting plate. After staining the cells, the cell morphology is observed under a microscope, and the hemoglobin concentration is detected by a special colorimeter. The cumbersome, low work efficiency, poor repeatability, and low detection parameters are far from meeting the actual work needs. In the 1950s, American scientist WHCoulter applied the resistance counting particle technology to blood cell testing. Through the combination of electrical impedance and photoelectric colorimetry, with the development of science and technology, many new Technology is applied to cell testing, such as impedance methods combined with laser scattering, cytochemical staining, high-frequency conductance and other technologies to form a new cell analyzer technology. Compared with the traditional method, the method realizes the automation of the cell counting, and greatly improves the working efficiency and the quality of the detection result. However, this method also has the defects of complicated structure, large volume and high cost, and is not suitable for the needs of rapid detection on site.

In order to meet the needs of rapid on-site detection, a centrifugal cell analyzer has appeared. The principle is to fill blood and other specimens into a capillary containing special reagents, centrifuge on a special centrifuge, and distribute the specific gravity of various components in the blood cells. Within different cell layers. The instrument is suitable for carrying, and does not require liquid reagents. It is very convenient for out-of-home use, but its existence cannot be accurately quantitatively analyzed, and fewer parameters can be detected, such as fatal defects, which are only used for initial screening tests at war and disaster sites, and cannot meet clinical requirements. need.

The microfluidic method integrates the liquid path and analysis circuit required by the Coulter principle cell counter on a microchip, which simplifies the structure of the instrument, but has the disadvantages of high cost of the reagent card and poor repeatability of the result. .

In order to meet the needs of rapid detection and analysis of cells and small particulate matter in the fields of disease diagnosis, environmental monitoring, food and drug safety, etc., there is an urgent need for a low cost, reliable results, simple operation, fast reporting and miniaturization of instruments. Device.

Summary of the invention

In order to overcome the shortcomings and deficiencies in the prior art, an object of the present invention is to provide a detecting device which is low in cost, reliable in results, simple in operation, quick in reporting, and capable of miniaturizing the instrument.

Another object of the present invention is to provide a cell detection method which is simple, rapid, and low in cost.

In order to solve the above technical problem, the present invention adopts the following technical solutions:

An apparatus for analyzing and detecting comprises a sample guiding tank body, a capillary tube and a collecting portion, wherein an upper end of the guiding sample tank body is open, and the guiding sample tank body comprises a plurality of side wall baffles connected end to end and an inclined bottom a lower end of the side wall baffle is connected to the bottom panel, the capillary is connected to a lower end of the bottom panel, the capillary is connected to the bottom panel, and the capillary and the collecting portion are inclined from a high end to a low position along an inclined direction of the bottom panel The bottom panel is provided with a first inlet opening through the bottom panel, and the liquid inlet of the capillary is in communication with the first inlet.

Further, a lower end of the capillary is provided with a detection guide head, and a liquid outlet of the capillary is opened at the detection lead.

Further, the bottom panel is provided with a second inlet through the bottom panel, the collecting portion is connected to the lower end of the bottom panel, and the collecting portion is provided with a flow guiding passage penetrating the upper end of the collecting portion and the lower end of the collecting portion, The second inlet is in communication with the liquid inlet of the flow channel.

Further, the analysis and detection device is connected to a lower end of the bottom panel, the collection portion is provided with a liquid collection chamber for collecting liquid, and the upper end of the liquid collection chamber is provided with a liquid inlet.

Further, the analysis and detection device further includes a detection pool detachably connected to the lower side of the capillary tube, the detection pool is surrounded by a bottom plate and a plurality of side plates, and the number of the capillary tubes is two In the above, a plurality of capillaries are arranged in the oblique direction of the bottom panel from the upper end to the lower end, and the detecting pool includes a plurality of detecting chambers for accommodating the detecting lead, and the two adjacent detecting chambers are provided with useful The first partition partitioning two adjacent detection chambers, the two adjacent detection guides, and the upper portions of the first partitions are respectively embedded in the corresponding card slots.

Further, the analysis and detection device further includes a detection pool detachably connected to the capillary and the lower portion of the collecting portion, the detection pool is surrounded by the bottom plate and the plurality of side plates, and the number of the capillary tubes For more than two, a plurality of capillary tubes are arranged in order from the upper end to the lower end in the oblique direction of the bottom panel, and the detecting pool includes a collecting chamber for accommodating the collecting portion and a plurality of detecting means for accommodating the detecting lead a first partition for separating two adjacent detection chambers is disposed between two adjacent detection chambers, and a portion for separating the collection chamber and the detection chamber is disposed between the collection chamber and the adjacent detection chamber a second partition, between the two adjacent detection guide heads, and between the collection portion and the adjacent detection guide head, a card slot with an opening facing downward is provided, and the upper portion and the second partition of the first partition The upper part of the board is respectively embedded in the corresponding card slot.

Further, the collection chamber is made of a light transmissive material.

Further, the area of the bottom plate located below the detection chamber is made of a light transmissive material.

Further, the inner wall of the capillary is made of a hydrophilic material.

Another object of the invention is achieved by the following technical solutions:

A method for detecting cells or particles by means of an analytical detection device, comprising the steps of:

(1) connecting the capillary tube and the collecting portion to the detecting chamber and the collecting chamber, respectively, and the collecting chamber and the plurality of detecting chambers are pre-placed with the reaction reagent;

(2) taking a liquid sample, and loading it from the end of the sample tank at the high end of the bottom panel into the sample guide tank;

(3) The liquid sample automatically fills the capillary after flowing through the first inlet, and all the remaining liquid samples enter the collection chamber through the flow guiding channel, and then react with the reagent in the collection chamber; the analysis and detection device is placed in a centrifugal device for centrifugation, capillary The liquid sample in the hole flows into the corresponding detection chamber and reacts with the reagents in the corresponding detection chamber respectively, and then the mixture after the reaction between the detection chamber and the collection chamber is analyzed and detected by using an external detection and analysis device.

The invention has the beneficial effects that the apparatus for analysis and detection of the invention integrates reagent pre-packaging and sample quantitative loading, and has the advantages of low cost, reliable result, simple operation, quick report and miniaturization of the instrument, and can be used for quantitative analysis.

The apparatus for analyzing and detecting according to the present invention can realize quantitative sample loading and detection of different detection items of needle cell or particle analysis, thereby making complicated manual operation simple; compared with the conventional analyzer, the instrument used with the present invention There is no need to set up reagent injection pipelines and pumps, sample loading needles, cleaning needles and other hardware facilities, so that the instrument structure is simple, the instrument cost is greatly reduced, and the analysis and detection of cells can be better popularized. The method is simple, fast and low in cost.

DRAWINGS

Fig. 1 is a cross-sectional view showing the apparatus for analyzing and detecting in the first embodiment.

FIG. 2 is a schematic perspective view showing the configuration of the detection device in the first embodiment without the detection pool.

Fig. 3 is a cross-sectional view showing that the detecting and detecting device is not provided with the detecting pool in the first embodiment.

4 is a schematic perspective view showing the structure of the detecting cell in the first embodiment.

Figure 5 is a cross-sectional view of the detecting cell in the first embodiment.

Fig. 6 is a cross-sectional view of the second embodiment.

Fig. 7 is a cross-sectional view showing the third embodiment.

Description of the reference signs:

11-side wall baffle; 12-first inlet; 13-capillary; 14-detection guide head; 15-flow channel; 16-collection; 17-pool; 18-second inlet 19-bottom panel; 21-guide channel body; 22-card slot; 23-detection chamber; 24-collection chamber; 25-side panel; 26-bottom panel; 27-first spacer; 28-second spacer ; 29 - first mounting portion; 31 - second mounting portion.

detailed description

In order to facilitate the understanding of those skilled in the art, the present invention will be further described in conjunction with the embodiments and the accompanying drawings, which are not intended to limit the invention.

Example 1

As shown in FIG. 1-3, an apparatus for analyzing and detecting includes a sample guiding tank 21, a capillary tube 13 and a collecting portion 16. The upper end of the guiding sample tank body 21 is open, and the sampling sample tank body 21 includes a plurality of a side wall baffle 11 and a bottom plate 19 which are disposed at an end and an end, and a bottom end of the bottom panel 19 respectively form a high end and a lower end, and a lower end of the side baffle 11 is connected to the bottom panel 19, The capillary tube 13 and the collecting portion 16 are both connected to the lower end of the bottom panel 19. The capillary tube 13 and the collecting portion 16 are sequentially arranged from the high end to the lower end in the oblique direction of the bottom panel 19, and the bottom panel 19 is opened through the bottom panel 19. a first inlet 12 and a second inlet 18 extending through the bottom panel 19, the inlet of the capillary 13 is in communication with the first inlet 12, and the inlet and the second inlet of the collecting portion 16 The sample port 18 is connected. The collecting portion 16 is provided with a flow guiding passage 15 penetrating the upper end of the collecting portion 16 and the lower end of the collecting portion 16, and the second inlet 18 is in communication with the liquid inlet of the flow guiding passage 15.

Further, the inner wall of the capillary 13 is made of a hydrophilic material. Further, the capillary 13 is set such that when in a stationary state, the liquid sample can fill the inner tube of the capillary 13, but the liquid sample does not overflow from the liquid outlet located at the lower end of the capillary 13.

The capillary 13 of the present invention is used for quantitative sampling, and the inner wall of the capillary 13 is made of a hydrophilic material, and the liquid sample is filled into the capillary 13 by capillary phenomenon to realize quantitative sampling, and the internal size and length of the capillary 13 are increased by the amount of the sample. The needs are different. The liquid sample can be placed in the first inlet of the bottom panel 19 into the capillary 13 and can enter the collection portion 16 through the second inlet 18 for the flow of the liquid sample.

As shown in FIGS. 1-3, the number of the capillary tubes 13 is two or more, and a plurality of the capillary tubes 13 are sequentially arranged from the upper end to the lower end in the oblique direction of the bottom panel 19. The liquid sample flowing through the bottom panel 19 can enter the capillary 13 from the first inlet 12 provided in the bottom panel 19.

As shown in FIG. 1-3, the lower end of the capillary 13 is provided with a detection lead 14, and the liquid outlet of the capillary 13 is opened at the detection lead 14. Further, two adjacent detection guide heads 14 are spaced apart; the collection portion 16 is spaced apart from the adjacent detection guide heads 14. Further, the detecting guide head 14 has a truncated cone shape or a prismatic shape, and the cross-sectional area of the detecting guiding head 146 is sequentially decreased from top to bottom. A person skilled in the art can set the shape of the detecting guide head 14 as needed.

As shown in FIG. 1-3, the sample guiding tank body 21 includes a plurality of side wall baffles 11 connected end to end. The lower end of the side wall baffle 11 is connected to the bottom panel 19, and the side wall baffle 11 is connected. The height of the upper end is higher than the height of the upper end of the bottom panel 19. The upper end of the side fence 11 is raised above the upper end of the bottom panel 19 to prevent liquid from flowing out of the sample guide body 21.

As shown in FIG. 1 , FIG. 4 and FIG. 5 , the analysis and detection device further includes a detection pool detachably connected to the lower portion 13 of the capillary tube and below the collection portion 16 , the detection pool being composed of the bottom plate 26 and The plurality of side plates 25 are provided, and the number of the capillary tubes 13 is two or more, and the plurality of the capillary tubes 13 are arranged in order from the upper end to the lower end in the oblique direction of the bottom panel 19. The liquid sample flowing through the bottom panel 19 can enter the capillary 13 from the first inlet 12 provided in the bottom panel 19. The detection pool includes a collection chamber 24 for accommodating the collection portion 16 and a plurality of detection chambers 23 for accommodating the detection guide heads 14. Two adjacent detection chambers 23 are provided for separating the two phases. A first partition 27 adjacent to the detection chamber 23, and a second partition 28 for separating the collection chamber 24 and the detection chamber 23 between the collection chamber 24 and the adjacent detection chamber 23, two adjacent detection guides A card slot 22 with an opening facing downward is disposed between the sample heads 14 and between the collecting portion 16 and the adjacent detecting guide heads 14. The upper portion of the first partition plate 27 and the upper portion of the second partition plate 28 are respectively used. It is embedded in the corresponding card slot 22.

In use, the reaction reagent is placed in the detection chamber 23 in advance, and the capillary 13 and the collection portion 16 are respectively inserted into the corresponding detection chamber 23 and the collection chamber 24, so that the detection pool is tightly connected to the lower side of the sample introduction tank 21; The liquid sample is loaded from the end of the sample tank body 21 at the high end of the bottom panel 19 into the sample guiding tank body 21, and the liquid sample flows from the high end to the low end in the oblique direction of the bottom panel 19, and the liquid sample flows through the liquid in turn. The first inlet 12 is filled with a corresponding capillary 13 for quantitative sampling under capillary action; the remaining liquid sample flows to the second inlet 18 at the lower end of the bottom panel 19, and via the flow channel 15 Enter the collection room 24. The collection chamber 24 can be used to hold excess sample, or the reaction reagent can be placed in the collection chamber 24 to perform item inspection of the liquid sample.

Further, a side of the collecting portion 16 away from the capillary 13 is provided with a first mounting portion 29 for connection with the side plate 25, and a side of the capillary 13 connected to the upper end of the bottom panel 19 away from the collecting portion 16 is provided for the side The second mounting portion 31 to which the plate 25 is connected, the first mounting portion 29 and the second mounting portion 31 are both stepped. When the lead assembly and the detecting pool are assembled, one side of the side plate 25 is connected to the first mounting portion 29, and one side of the other side plate 25 opposite to the side plate 25 is connected to the second mounting portion 31, so that detection is performed. The pool is closely connected to the lower side of the sample guide body 21, and the alignment is accurate.

The device for analysis and detection of the invention integrates reagent pre-packaging, sample loading and parameter detection, and the detection room can be used for, but not limited to, counting red blood cells, white blood cells, platelets, cell size measurement and morphological analysis in blood. The collection chamber can be used for, and is not limited to, measurement of hemoglobin concentration and the like.

Further, the collection chamber is made of a light transmissive material. Preferably, the collection chamber 24 has a rectangular cross section, and both side walls of the collection chamber 24 adjacent to the second partition 28 are made of a light transmissive material. The mixture in the collection chamber 24 may be subjected to a colorimetric treatment or the like using a device such as a spectrophotometer.

Further, the area of the bottom plate 26 located below the detection chamber 23 is made of a light transmissive material. Further, the thickness of the region of the bottom plate 26 at the lower end of the detecting chamber 23 is not more than 5 mm. Further, the thickness of the bottom plate 26 located at the lower end of the detection chamber 23 is 0.1-2 mm. It is convenient to observe and analyze the formed components in the sample deposited on the bottom plate using a microscope.

Preferably, the inner diameter of the flow guiding passage 15 is larger than the inner diameter of the capillary tube 13. The inner diameter of the collecting portion 16 may be, but not limited to, 0.5-10 mm. Due to the arrangement of the above structure, the liquid sample left by the bottom panel 19 can flow into the lower collection chamber 24 through the flow guiding passage 15 without being centrifuged or oscillated. Those skilled in the art can set the inner diameter of the flow guiding passage 15 to be smaller than or equal to the inner diameter of the capillary tube 13 as needed.

In this embodiment, a blood cell detection method is taken as an example, and a detection method for detecting cells or particles by using an analysis and detection device is provided, which comprises the following steps:

(1) The capillary tube 13 and the collecting portion 16 are respectively inserted into the corresponding detecting chamber 23 and the collecting chamber 24, and the plurality of detecting chambers 23 and the collecting chamber 24 are preliminarily placed with a reaction reagent;

(2) taking the diluted liquid sample, and loading the sample from the high end of the bottom plate 19 into the sample guiding tank, the liquid sample flows from the high end to the low end in the oblique direction of the bottom panel 19, and the liquid sample flows through When the first first inlet 12 is filled, the capillary 13 for quantitative sampling is filled under the action of capillary phenomenon, and the remaining liquid sample sequentially flows into the remaining first inlet 12 and enters the remaining capillary 13; the remaining liquid The sample flows to the second inlet 18 at the lower end of the bottom panel 19 and enters the collection chamber 24 via the flow channel 15;

(3) The liquid sample automatically fills the capillary 13 after flowing through the first inlet, and all the remaining liquid samples enter the collection chamber 24 through the flow channel 15, and react with the reagent in the collection chamber 24; The device is centrifuged, and the liquid sample in the capillary hole 13 flows into the corresponding detection chamber 23, and reacts with the reagent in the corresponding detection chamber 23, respectively, and then the mixture of the detection chamber 23 and the collection chamber 24 is reacted by an external detection and analysis device. Perform analytical testing.

Further, the detection chamber 23 can be used for detecting the formation of cells or particles, and counting and morphological analysis of the cells or particles having a composition is performed from below the detection chamber using an inverted microscope. Further, after the analysis and detection device is centrifuged, the blood cells or particles in the detection chamber after the reaction with the reaction reagent are settled on the bottom plate. Since the bottom plate is made of a transparent material, an inverted microscope can be used from the detection chamber. Counting and morphological analysis of cells or particles is performed below. The collection chamber 24 is made of a transparent material and can be used for colorimetric detection. The reaction mixture is spectrophotometrically used for concentration analysis of the components. As shown in Fig. 4, the direction of the arrow shown in the figure indicates the direction in which light is transmitted when the counter mixture is collected in the collection chamber 24 by spectrophotometry.

The apparatus for analyzing and detecting according to the present invention can realize quantitative sampling and detection of different detection items for blood cell analysis, which makes complicated manual operation simple, can detect different detection items at one time or perform multiple detections on the same detection item. Compared with the traditional blood cell analyzer, the instrument used in conjunction with the present invention does not need to provide a reagent injection pipe and a pump, a sample needle, a cleaning needle and other hardware facilities, thereby making the instrument structure simple and capable of Reduce the cost of the instrument, so that the analysis and detection of blood cells can be better spread to the community and primary health care institutions.

When the blood test is performed by the analysis and detection device of the present invention, the operation is convenient and quick. The apparatus for analysis and detection of the present invention is generally made of a disposable material, and is discarded after the measurement is completed, and the sample guide tank 21, the capillary tube 13 and the collecting portion 16 need not be cleaned, so that the apparatus for operating the analysis and detecting device does not require a design liquid. Road system, external reagent barrel, waste liquid barrel, etc., also do not need to design the needle, eliminating the steps of cleaning the needle, greatly simplifying the structure of the instrument, improving the portability and ease of use of the instrument. Detection and particle analysis, etc., are of great significance in achieving rapid bedside diagnosis.

Example 2

As shown in FIG. 6, an apparatus for analyzing and detecting includes a sample guiding tank body 21, a capillary tube 13 and a collecting portion 16, and an upper end of the sampling sample tank body 21 is open, and the sampling sample tank body 21 includes a plurality of head and tail portions. a connecting side wall baffle 11 and an inclined bottom plate 19, the two ends of the bottom plate 19 respectively forming a high end and a low end, and the lower end of the side baffle 11 is connected with the bottom panel 19, the capillary 13 Connected to the lower end of the bottom panel 19, the capillary tube 13 is connected to the bottom panel 19. The capillary tube 13 and the collecting portion 16 are arranged in the oblique direction of the bottom panel 19 from the high end to the low end, and the bottom panel 19 is opened through the bottom. The first inlet 12 of the panel 19, the inlet of the capillary 13 is in communication with the first inlet 13, and the collecting portion 16 is for collecting liquid.

The collecting portion 16 is connected to the lower end of the bottom panel 19, and the collecting portion 16 is provided with a liquid collecting chamber 17 for collecting liquid, and the upper end of the liquid collecting chamber 17 is provided with a liquid inlet. In use, the liquid sample is filled with the capillary 13 and the residual liquid flowing down from the bottom panel 19 can enter the liquid collection chamber 17 through the liquid inlet to function as a residual liquid.

Example 3

As shown in FIG. 7, the lower end of the capillary tube 13 is provided with a detection lead-in head 14, and the liquid outlet of the capillary tube 13 is opened in the detection lead-in head 14. The apparatus for analyzing and detecting further includes a detecting pool detachably connected to the lower side of the capillary tube 13, the detecting pool is surrounded by the bottom plate 26 and the plurality of side plates 25, and the number of the capillary tubes 13 is two More than one or more of the plurality of the capillary tubes 13 are arranged in the oblique direction of the bottom panel 19 from the upper end to the lower end, and the detection pool includes a plurality of detection chambers 23 for accommodating the detection guide head 13, the detection chamber 23 is in communication with the capillary 13, and a first partition 27 for separating two adjacent detection chambers 23 is disposed between two adjacent detection chambers 23, between two adjacent detection guide heads 13, and a collection portion 16 A card slot 22 having an opening facing downward is disposed between the adjacent detection lead heads 13 , and upper portions of the first spacers 27 are respectively embedded in the corresponding card slots 22 .

Preferably, between the two adjacent detection guide heads 13 and between the collection portion 16 and the adjacent detection guide heads 13, a card slot 22 having an opening downward is provided, and the first spacer 27 is The card slots 22 between the two adjacent detection heads 13 are in one-to-one correspondence. The upper portions of the first partitions 27 are respectively embedded in the corresponding card slots 22; the detection pool is away from the side of the high end of the bottom panel 19. The plate 25 can be embedded in the card slot 22 between the collecting portion 16 and the adjacent detecting guide head 13.

In use, a reaction reagent is placed in the detection chamber 23 in advance, and the capillary 13 is inserted into the corresponding detection chamber 23 to closely connect the detection pool to the lower side of the sample introduction tank 21; the liquid sample to be tested is taken from the sample introduction tank 21 The end of the high end of the bottom panel 19 is loaded into the sample guiding body 21, and the liquid sample flows from the high end to the low end in the oblique direction of the bottom panel 19, and the liquid sample sequentially flows through the plurality of first inlets 12, Under the action of capillary phenomenon, the corresponding capillary 13 for quantitative sampling is filled; the residual liquid flowing down from the bottom panel 19 can enter the liquid collection chamber 17 through the liquid inlet to collect the residual liquid. Preferably, the liquid collection chamber 17 is made of a transparent material. In use, the reagent is placed in the liquid collection chamber 17 in advance, and after the reagent is reacted with the sample, the absorbance can be measured spectrophotometrically.

The rest of the embodiment is the same as that of Embodiment 2, and details are not described herein again.

The above-described embodiments are a preferred embodiment of the present invention, and the present invention can be implemented in other ways, and any obvious alternatives are within the scope of the present invention without departing from the spirit of the present invention.

Claims

The device for analyzing and detecting is characterized in that it comprises a sample guiding tank body, a capillary tube and a collecting portion, wherein the upper end of the guiding sample tank body is open, and the guiding sample tank body comprises a plurality of side wall baffles which are connected end to end and a bottom panel disposed obliquely, a lower end of the side wall baffle is connected to the bottom panel, the capillary is connected to a lower end of the bottom panel, the capillary is connected to the bottom panel, and the capillary and the collecting portion are inclined along the bottom panel The high end is arranged to the lower end, and the bottom panel is provided with a first inlet through the bottom panel, and the inlet of the capillary is in communication with the first inlet.
The apparatus for analysis and detection according to claim 1, wherein a lower end of the capillary is provided with a detection lead, and a liquid outlet of the capillary is opened in the detection lead.
The apparatus for analyzing and detecting according to claim 1, wherein the bottom panel is provided with a second injection port penetrating through the bottom panel, the collection portion is connected to a lower end of the bottom panel, and the collecting portion is provided with a through portion. An upper end of the collecting portion and a flow guiding passage at a lower end of the collecting portion, the second inlet being in communication with a liquid inlet of the flow guiding passage.
The apparatus for analyzing and detecting according to claim 1, wherein the collecting portion is connected to a lower end of the bottom panel, the collecting portion is provided with a liquid collecting chamber for collecting liquid, and an upper end of the liquid collecting chamber is provided. There is a liquid inlet.
The apparatus for analyzing and detecting according to claim 1, further comprising a detection pool detachably connected to the lower side of the capillary tube, wherein the detection pool is surrounded by a bottom plate and a plurality of side plates. The number of the capillaries is two or more, and the plurality of capillaries are arranged in the oblique direction of the bottom panel from the high end to the low end, and the detecting pool includes a plurality of detecting chambers for accommodating the detecting lead, two adjacent A first partition for separating two adjacent detection chambers is disposed between the detection chambers, and two adjacent detection guide heads are provided with a card slot with an opening facing downward, and the upper portions of the first partitions are respectively It is used to embed in the corresponding card slot.
The apparatus for analyzing and detecting according to claim 3, further comprising a detection pool detachably connected to the capillary tube and the lower portion of the collecting portion, the detection pool being surrounded by the bottom plate and the plurality of side plates The number of the capillaries is two or more, and the plurality of capillaries are arranged in the oblique direction of the bottom panel from the upper end to the lower end, and the detecting pool includes a collecting chamber for accommodating the collecting portion and a plurality of capacitors for receiving a detection chamber for detecting a lead guide is provided, and a first partition for separating two adjacent detection chambers is disposed between two adjacent detection chambers, and the collection chamber is disposed between the adjacent detection chambers and the adjacent detection chambers a second partition separating the collection chamber and the detection chamber, between the two adjacent detection guide heads, and between the collection portion and the adjacent detection guide head, a card slot having an opening facing downward is provided, the first The upper portion of the partition and the upper portion of the second partition are respectively embedded in the corresponding card slots.
The apparatus for analysis and detection according to claim 6, wherein the collection chamber is made of a light transmissive material.
The apparatus for analysis and detection according to claim 5, wherein the area of the bottom plate located below the detection chamber is made of a light transmissive material.
The apparatus for analysis and detection according to claim 1, wherein the inner wall of the capillary is made of a hydrophilic material.
A method for detecting a cell or a particle by using the apparatus for analyzing and detecting according to claim 6, comprising the steps of:

(1) connecting the capillary tube and the collecting portion to the detecting chamber and the collecting chamber, respectively, and the collecting chamber and the plurality of detecting chambers are pre-placed with the reaction reagent;

(2) taking a liquid sample, and loading it from the end of the sample tank at the high end of the bottom panel into the sample guide tank;

(3) The liquid sample automatically fills the capillary after flowing through the first inlet, and all the remaining liquid samples enter the collection chamber through the flow guiding channel, and then react with the reagent in the collection chamber; the analysis and detection device is placed in a centrifugal device for centrifugation, capillary The liquid sample in the hole flows into the corresponding detection chamber and reacts with the reagents in the corresponding detection chamber respectively, and then the mixture after the reaction between the detection chamber and the collection chamber is analyzed and detected by using an external detection and analysis device.