CN108362615B - Device for analysis and detection and cell or particle detection method - Google Patents
Device for analysis and detection and cell or particle detection method Download PDFInfo
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- CN108362615B CN108362615B CN201810404763.0A CN201810404763A CN108362615B CN 108362615 B CN108362615 B CN 108362615B CN 201810404763 A CN201810404763 A CN 201810404763A CN 108362615 B CN108362615 B CN 108362615B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
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- G01N15/01—
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the field of analysis and detection, in particular to an analysis and detection device and a cell or particle detection method, wherein the analysis and detection device comprises a sample guide groove body, a capillary tube and a collection part, the sample guide groove body comprises a plurality of side wall baffles and a bottom plate which are connected end to end in sequence, the lower ends of the side wall baffles are connected with the bottom plate, the capillary tube is connected to the lower end of the bottom plate, the capillary tube is connected to the bottom plate, the capillary tube and the collection part are arranged from a high-position end to a low-position end along the inclined direction of the bottom plate, the bottom plate is provided with a first sample inlet penetrating through the bottom plate, and the liquid inlet of the capillary tube is communicated with the first sample inlet. The device for analysis and detection can be used for quantitative detection and analysis of cells or particles, and has the advantages of simple structure, convenient operation, simplified structure of an operation instrument and the like.
Description
Technical Field
The invention relates to the field of analysis and detection, in particular to a device for analysis and detection and a method for detecting cells or particles.
Background
With the continuous progress of science and technology, the trend of the detection and analysis device to two directions is that: one is to develop towards the direction of higher, precise and integrated, the direction depends on large-scale full-automatic instrument equipment, and the method has the advantages of high batch detection speed, accurate result and the like, but also has the defects of high instrument price, complex operation, long single sample reporting time and the like; the other is to develop towards the direction of 'simple, convenient and quick', and is characterized by realizing the miniaturization of the instrument, simple and convenient operation and quick report, and the instrument has low instrument cost and simple sample treatment and is suitable for on-site quick detection although the batch processing capability of the instrument is not as good as that of a large-scale full-automatic instrument.
In the fields of disease diagnosis, environmental monitoring, food and medicine safety and the like, the method is very important to carry out on-site rapid quantitative detection and analysis on cells and small particulate matters. In the field, the traditional method adopts a Bob (Neubauer) counting plate to count the number of cells under a microscope, the cell morphology is observed under the microscope after the cells are dyed, and a special colorimeter is adopted to detect the hemoglobin concentration, so that the method has the defects of complex operation, low working efficiency, poor repeatability and few detection parameters, and can not meet the actual working needs. In the 50 s of the 20 th century, the U.S. scientist coulter (w.h. coulter) applied the resistance method counting particle technique to blood cell inspection work, and by the principle of combining an electrical impedance method with photoelectric colorimetry, along with the development of scientific technology, many new techniques are applied to cell inspection work, such as combining an impedance method with laser scattering, cytochemical staining, high-frequency conductance and other techniques, so as to form a new cell analyzer technique. Compared with the traditional method, the method realizes the automation of cell counting, and greatly improves the working efficiency and the quality of detection results. However, the method also has the defects of complex instrument structure, huge volume, high cost and the like, and is not suitable for the requirement of on-site rapid detection.
In order to meet the requirement of on-site rapid detection, a centrifugal cell analyzer is developed, and the principle is that a sample such as blood is filled into a capillary tube containing a special reagent, and the sample is centrifuged on a special centrifuge, so that the specific gravity of various components in blood cells is different and distributed in different cell layers. The instrument is suitable for carrying, does not need liquid reagent, is very convenient for external use, but has the fatal defects of inaccurate quantitative analysis, few detectable parameters and the like, is only used for primary screening detection in war and disaster sites, and cannot meet clinical requirements.
The microfluidic method integrates a liquid path and an analysis circuit required by a cell counter based on the Coulter principle on a microchip, thereby achieving the purpose of simplifying the structure of the instrument, but has the defects of high cost of a reagent card, poor repeatability of results and the like.
In order to meet the requirements of on-site rapid detection and analysis of cells and small particulate matters in the fields of disease diagnosis, environmental monitoring, food and drug safety and the like, a detection device which is low in cost, reliable in result, simple to operate, rapid in reporting and capable of achieving instrument miniaturization is urgently needed.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the invention aims to provide a detection device which is low in cost, reliable in result, simple to operate, quick in report and capable of achieving instrument miniaturization.
Another object of the present invention is to provide a cell detection method that is easy and quick to operate and has low cost.
In order to solve the technical problems, the invention adopts the following technical scheme:
the utility model provides an analysis detects and uses device, includes guide appearance cell body, capillary and collecting portion, the upper end opening of guide appearance cell body, guide appearance cell body includes the side wall baffle that a plurality of heads and tails connect gradually and the bottom plate that the slope set up, the lower extreme and the bottom plate of side wall baffle are connected, the capillary is connected in the lower extreme of bottom plate, the capillary is connected in the bottom plate, capillary and collecting portion are arranged by high-order end to low-order end along the incline direction of bottom plate, the bottom plate has seted up the first inlet that runs through the bottom plate, the inlet and the first inlet intercommunication of capillary.
Further, the lower end of the capillary tube is provided with a detection sample guide head, and a liquid outlet of the capillary tube is arranged on the detection sample guide head.
Further, the bottom plate is provided with a second sample inlet penetrating through the bottom plate, the collecting part is connected to the lower end of the bottom plate, the collecting part is provided with a flow guide channel penetrating through the upper end of the collecting part and the lower end of the collecting part, and the second sample inlet is communicated with a liquid inlet of the flow guide channel.
Further, the collecting part is connected to the lower end of the bottom panel, the collecting part is provided with a liquid collecting chamber for collecting liquid, and the upper end of the liquid collecting chamber is provided with a liquid inlet.
Further, the device for analysis and detection still includes the detection pond, the below in capillary is connected in the detachable way in detection pond, the detection pond is enclosed by bottom plate and a plurality of curb plate and is established, the quantity of capillary is more than two, and a plurality of capillaries are arranged in proper order by the high-order end to the low-order end along the incline direction of bottom plate, the detection pond includes a plurality of detection rooms that are used for holding and detect the sample guide head, is provided with the first baffle that is used for separating two adjacent detection rooms between two adjacent detection rooms, the draw-in groove between two adjacent detection sample guide heads, the upper portion of first baffle is used for inlaying respectively and locates corresponding draw-in groove.
Further, the device for analysis and detection still includes the detection pond, the below of connection in capillary and collecting part can be dismantled to the detection pond, the detection pond is enclosed by bottom plate and a plurality of curb plate and is established, the quantity of capillary is more than two, and a plurality of capillaries are arranged in proper order by high-order end to low-order end along the incline direction of bottom plate, the detection pond is including the collecting chamber that is used for holding the collecting part and a plurality of detection room that is used for holding the detection and leads the appearance head, is provided with the first baffle that is used for separating two adjacent detection rooms between two adjacent detection rooms, be provided with the second baffle that is used for separating collecting room and detection room between collecting room and the adjacent detection room, all be provided with the draw-in groove that the opening was down between two adjacent detection lead appearance heads, the upper portion of first baffle and the upper portion of second baffle are used for inlaying respectively and are located corresponding draw-in groove.
Further, the collection chamber is made of a light-transmitting material.
Further, the area of the bottom plate below the detection chamber is made of a light-transmitting material.
Further, the inner wall of the capillary tube is made of a hydrophilic material.
The other object of the invention is achieved by the following technical scheme:
a detection method for detecting cells or particles by using an analytical detection device, comprising the steps of:
(1) Connecting the capillary and the collection part with the detection chamber and the collection chamber respectively, wherein the collection chamber and the plurality of detection chambers are pre-provided with a reaction reagent;
(2) Taking a liquid sample, and adding the sample from one end of the sample guide groove body, which is positioned at the high end of the bottom panel, into the sample guide groove body;
(3) The liquid sample flows through the first sample inlet and then automatically fills the capillary tube, and the rest liquid sample completely enters the collecting chamber through the diversion channel and then reacts with the reagent in the collecting chamber; and (3) placing the device for analysis and detection in a centrifugal device for centrifugation, enabling the liquid sample in the capillary holes to flow into corresponding detection chambers and react with the reagents in the corresponding detection chambers respectively, and then adopting external detection and analysis equipment to analyze and detect the mixture after the reaction of the detection chambers and the collection chambers.
The invention has the beneficial effects that: the device for analysis and detection integrates reagent pre-packaging and sample quantitative loading, has low cost, reliable result, simple operation and quick report, can realize instrument miniaturization, and can be used for quantitative analysis.
The quantitative sample adding and detection of different detection items of needle cell or particle analysis can be realized by the analysis and detection device, so that complicated manual operation is simplified; compared with the traditional analyzer, the analyzer matched with the invention does not need to be provided with a reagent sampling pipeline, a pump, a sample loading needle, a cleaning loading needle and other hardware facilities, so that the structure of the analyzer is simple, the cost of the analyzer is greatly reduced, the analysis and detection of cells can be better popularized, and the detection method is simple and convenient to operate and low in cost.
Drawings
FIG. 1 is a cross-sectional view of the analysis and detection apparatus in example 1.
FIG. 2 is a schematic perspective view showing the structure of the analytical test device according to example 1 without a test cell.
FIG. 3 is a cross-sectional view showing the analytical test device of example 1 without a test cell.
FIG. 4 is a schematic perspective view of the detection cell in example 1.
FIG. 5 is a cross-sectional view of the test cell in example 1.
Fig. 6 is a cross-sectional view of example 2.
Fig. 7 is a cross-sectional view of example 3.
Reference numerals illustrate:
11-side wall baffles; 12-a first sample inlet; 13-capillary; 14-detecting a sample guide head; 15-a diversion channel; 16-a collection section; 17-collecting liquid pool; 18-a second sample inlet; 19-a bottom panel; 21-a sample guide groove body; 22-clamping grooves; 23-a detection chamber; 24-a collection chamber; 25-side plates; 26-a bottom plate; 27-a first separator; 28-a second separator; 29-a first mounting portion; 31-a second mounting portion.
Detailed Description
The present invention will be further described with reference to examples and drawings for the purpose of facilitating understanding of those skilled in the art, and reference to the embodiments is not intended to limit the invention.
Example 1
As shown in fig. 1-3, an analysis and detection device comprises a sample guiding groove body 21, a capillary tube 13 and a collecting part 16, wherein the upper end of the sample guiding groove body 21 is opened, the sample guiding groove body 21 comprises a plurality of side wall baffles 11 and a bottom panel 19 which are connected end to end in sequence, the two ends of the bottom panel 19 respectively form a high-position end and a low-position end, the lower end of the side wall baffles 11 is connected with the bottom panel 19, the capillary tube 13 and the collecting part 16 are connected to the lower end of the bottom panel 19, the capillary tube 13 and the collecting part 16 are sequentially arranged from the high-position end to the low-position end along the inclined direction of the bottom panel 19, the bottom panel 19 is provided with a first sample inlet 12 penetrating through the bottom panel 19 and a second sample inlet 18 penetrating through the bottom panel 19, the liquid inlet of the capillary tube 13 is communicated with the first sample inlet 12, and the liquid inlet of the collecting part 16 is communicated with the second sample inlet 18. The collecting part 16 is provided with a diversion channel 15 penetrating through the upper end of the collecting part 16 and the lower end of the collecting part 16, and the second sample inlet 18 is communicated with a liquid inlet of the diversion channel 15.
Further, the inner wall of the capillary 13 is made of a hydrophilic material. Further, the capillary 13 is set so that the liquid sample can fill the inner tube of the capillary 13 when it is in a stationary state, but the liquid sample does not overflow from the liquid outlet at the lower end of the capillary 13.
The capillary tube 13 is used for quantitative sampling, the inner wall of the capillary tube 13 is made of hydrophilic materials, liquid samples are filled in the capillary tube 13 through capillary phenomenon, quantitative sampling is achieved, and the size and the length of the inner dimension of the capillary tube 13 are different according to the requirement of the sample adding amount. The liquid sample may be introduced into the capillary 13 through a first inlet provided in the bottom plate 19 and may be introduced into the collection portion 16 through a second inlet 18, and the collection portion 16 is configured to allow the liquid sample to flow therethrough.
As shown in fig. 1 to 3, the number of the capillaries 13 is two or more, and the capillaries 13 are arranged in order from the high end to the low end in the inclined direction of the bottom plate 19. The liquid sample flowing through the bottom plate 19 can enter the capillary 13 through the first sample inlet 12 provided on the bottom plate 19.
As shown in fig. 1-3, a detection sample guide head 14 is disposed at the lower end of the capillary 13, and a liquid outlet of the capillary 13 is disposed at the detection sample guide head 14. Further, two adjacent detection sample guide heads 14 are arranged at intervals; the collection unit 16 is spaced apart from the adjacent detection sample head 14. Further, the detection sample guiding head 14 is in a shape of a truncated cone or a truncated pyramid, and the cross-sectional area of the detection sample guiding head 14 6 is sequentially reduced from top to bottom. The shape of the test sample head 14 can be set as desired by those skilled in the art.
As shown in fig. 1-3, the sample guiding slot 21 includes a plurality of side wall baffles 11 connected end to end in sequence, the lower end of the side wall baffle 11 is connected with the bottom panel 19, and the upper end of the side wall baffle 11 is higher than the upper end of the bottom panel 19. The upper end of the side wall baffle 11 is higher than the upper end of the bottom plate 19, so that the liquid can be prevented from flowing out of the sample guide groove body 21.
As shown in fig. 1, 4 and 5, the analysis and detection device further includes a detection cell detachably connected to the lower portion 13 of the capillary tube and the lower portion of the collection portion 16, the detection cell is surrounded by a bottom plate 26 and a plurality of side plates 25, the number of the capillary tubes 13 is two or more, and the plurality of capillary tubes 13 are sequentially arranged from a high position end to a low position end along the inclination direction of the bottom plate 19. The liquid sample flowing through the bottom plate 19 can enter the capillary 13 through the first sample inlet 12 provided on the bottom plate 19. The detection pool comprises a collection chamber 24 for accommodating the collection part 16 and a plurality of detection chambers 23 for accommodating the detection sample guide heads 14, a first partition plate 27 for separating the two adjacent detection chambers 23 is arranged between the two adjacent detection chambers 23, a second partition plate 28 for separating the collection chamber 24 and the detection chambers 23 is arranged between the collection chamber 24 and the adjacent detection chambers 23, clamping grooves 22 with downward openings are respectively arranged between the two adjacent detection sample guide heads 14 and between the collection part 16 and the adjacent detection sample guide heads 14, and the upper parts of the first partition plate 27 and the second partition plate 28 are respectively used for being embedded in the corresponding clamping grooves 22.
When in use, the reaction reagent is placed in the detection chamber 23 in advance, and the capillary 13 and the collection part 16 are respectively inserted into the corresponding detection chamber 23 and the collection chamber 24, so that the detection cell is tightly connected below the sample guide groove body 21; taking a liquid sample to be measured, adding a sample from one end of the sample guide groove body 21, which is positioned at the high end of the bottom panel 19, into the sample guide groove body 21, enabling the liquid sample to flow from the high end to the low end along the inclined direction of the bottom panel 19, and filling the capillary tube 13 for quantitative sampling under the action of capillary phenomenon when the liquid sample sequentially flows through the first sample inlets 12; the remaining liquid sample flows to the second sample inlet 18 at the lower end of the bottom plate 19 and enters the collection chamber 24 via the flow guide channel 15. The collection chamber 24 may be used to hold excess sample, or a reagent may be placed in advance in the collection chamber 24 to perform a project test of the liquid sample.
Further, a first mounting portion 29 for connecting with the side plate 25 is provided on a side of the collecting portion 16 away from the capillary tube 13, a second mounting portion 31 for connecting with the side plate 25 is provided on a side of the capillary tube 13 connected to the high end of the bottom plate 19 away from the collecting portion 16, and the first mounting portion 29 and the second mounting portion 31 are stepped. When the sample guiding assembly and the detection tank are assembled, one side of the side plate 25 is connected with the first mounting part 29, and one side of the other side plate 25 opposite to the side plate 25 is connected with the second mounting part 31, so that the detection tank is tightly connected to the lower part of the sample guiding groove body 21, and the alignment is accurate.
The device for analysis and detection of the invention integrates reagent pre-packaging, sample loading and parameter detection, can be used in a detection chamber for but not limited to counting red blood cells, white blood cells and platelets in blood, measuring cell size and morphological analysis, and can be used in a collection chamber for but not limited to measuring hemoglobin concentration and the like.
Further, the collection chamber is made of a light-transmitting material. Preferably, the cross section of the collecting chamber 24 is rectangular, and both side walls of the collecting chamber 24 adjacent to the second partition 28 are made of a light-transmitting material. The mixture in the collection chamber 24 may be subjected to colorimetric processing using a spectrophotometer or the like.
Further, the area of the bottom plate 26 located below the detection chamber 23 is made of a light-transmitting material. Further, the thickness of the region of the bottom plate 26 at the lower end of the detection chamber 23 is not more than 5mm. Further, the thickness of the region of the bottom plate 26 at the lower end of the detection chamber 23 is 0.1-2mm. The microscope is convenient for observing and analyzing the formed components in the sample deposited on the bottom plate.
Preferably, the inner diameter of the diversion channel 15 is larger than the inner diameter of the capillary 13. The inner diameter of the collecting portion 16 may be, but is not limited to, 0.5-10mm. Due to the arrangement of the above-described structure, the liquid sample left by the bottom plate 19 can flow into the collecting chamber 24 below through the flow guide passage 15 without centrifugation or shaking. The inner diameter of the diversion channel 15 can be set to be smaller than or equal to the inner diameter of the capillary tube 13 as required by the person skilled in the art.
In this embodiment, taking blood cell detection as an example, there is provided a detection method for detecting cells or particles by using an analytical detection device, comprising the steps of:
(1) The capillary 13 and the collection unit 16 are inserted into the corresponding detection chamber 23 and collection chamber 24, respectively, and the plurality of detection chambers 23 and collection chambers 24 are provided with a reaction reagent in advance;
(2) Taking a diluted liquid sample, adding the sample from one end of the sample guide groove, which is positioned at the high end of the bottom panel 19, into the sample guide groove, enabling the liquid sample to flow from the high end to the low end along the inclined direction of the bottom panel 19, filling the capillary tube 13 for quantitative sampling under the action of capillary phenomenon when the liquid sample flows through the first sample inlet 12, and enabling the rest of the liquid sample to sequentially flow into the rest of the first sample inlets 12 and enter the rest of the capillary tubes 13; the rest liquid sample flows to a second sample inlet 18 positioned at the lower end of the bottom panel 19 and enters a collecting chamber 24 through a diversion channel 15;
(3) The capillary 13 is automatically filled after the liquid sample flows through the first sample inlet, and the residual liquid sample completely enters the collecting chamber 24 through the diversion channel 15 and reacts with the reagent in the collecting chamber 24; the analysis and detection device is placed in a centrifugal device for centrifugation, the liquid sample in the capillary holes 13 flows into the corresponding detection chambers 23 and reacts with the reagents in the corresponding detection chambers 23 respectively, and then the mixture after the reaction of the detection chambers 23 and the collection chambers 24 is analyzed and detected by adopting external detection and analysis equipment.
Further, the detection chamber 23 can be used for detecting the formation of cells or particles, and the counting and morphological analysis of the physical components of the cells or particles can be performed from below the detection chamber by using an inverted microscope. Further, after the centrifugation treatment, the blood cells or particles after the reaction with the reagent in the detection chamber of the analysis and detection device are settled on the bottom plate, and the bottom plate is made of transparent material, so that the counting and morphological analysis of the cells or particles can be performed from the lower side of the detection chamber by using an inverted microscope. The collection chamber 24 is made of a transparent material and can be used for colorimetric detection, and the reacted mixture is subjected to concentration analysis of components by spectrophotometry. As shown in FIG. 4, the direction of the arrows in the figure indicate the direction of light transmission when spectrophotometrically measuring the reverse mixture in the collection chamber 24.
The quantitative sample adding and detecting of different detection items aiming at blood cell analysis can be realized through the analysis detection device, so that complex manual operation is simplified, and different detection items can be detected once or the same detection item can be detected for multiple times; compared with the traditional blood cell analyzer, the blood cell analyzer does not need to be provided with a reagent sample injection pipeline, a pump, a sample injection needle, a cleaning sample injection needle and other hardware facilities, so that the structure of the blood cell analyzer is simplified, the cost of the blood cell analyzer can be greatly reduced, and the blood cell analyzer is better popularized to communities and basic medical institutions.
When the device for analysis and detection is used for blood detection, the operation is convenient and quick. The analysis and detection device is generally made of disposable materials, and is discarded after measurement is finished, so that the sample guide groove body 21, the capillary tube 13 and the collecting part 16 are not required to be cleaned, an instrument for operating the analysis and detection device is not required to be provided with a liquid path system, an external reagent barrel, a waste liquid barrel and the like, a sample adding needle is not required to be provided, the step of cleaning the sample adding needle and the like is avoided, the structure of the instrument is greatly simplified, the portability and usability of the instrument are improved, and the analysis and detection device has great significance in the aspects of realizing bedside rapid diagnosis and the like for blood detection, particle analysis and the like.
Example 2
As shown in fig. 6, an analysis and detection device comprises a sample guiding groove body 21, a capillary tube 13 and a collecting part 16, wherein the upper end of the sample guiding groove body 21 is provided with an opening, the sample guiding groove body 21 comprises a plurality of side wall baffles 11 which are sequentially connected end to end and a bottom panel 19 which is obliquely arranged, two ends of the bottom panel 19 respectively form a high-position end and a low-position end, the lower end of the side wall baffles 11 is connected with the bottom panel 19, the capillary tube 13 is connected with the lower end of the bottom panel 19, the capillary tube 13 is connected with the bottom panel 19, the capillary tube 13 and the collecting part 16 are arranged from the high-position end to the low-position end along the oblique direction of the bottom panel 19, the bottom panel 19 is provided with a first sample inlet 12 penetrating through the bottom panel 19, the liquid inlet of the capillary tube 13 is communicated with the first sample inlet 13, and the collecting part 16 is used for collecting liquid.
The collecting part 16 is connected to the lower end of the bottom plate 19, the collecting part 16 is provided with a liquid collecting chamber 17 for collecting liquid, and the upper end of the liquid collecting chamber 17 is provided with a liquid inlet. When in use, the capillary 13 is filled with liquid sample, and the residual liquid flowing down from the bottom plate 19 can enter the liquid collecting chamber 17 through the liquid inlet, so as to collect the residual liquid.
Example 3
As shown in fig. 7, a detection sample guide head 14 is disposed at the lower end of the capillary 13, and a liquid outlet of the capillary 13 is opened at the detection sample guide head 14. The device for analysis and detection further comprises a detection tank, the detection tank is detachably connected to the lower portion of the capillary tube 13, the detection tank is formed by surrounding a bottom plate 26 and a plurality of side plates 25, the number of the capillary tubes 13 is more than two, the capillary tubes 13 are sequentially arranged from a high position end to a low position end along the inclined direction of the bottom plate 19, the detection tank comprises a plurality of detection chambers 23 used for accommodating the detection sample guide heads 13, the detection chambers 23 are communicated with the capillary tubes 13, a first partition plate 27 used for separating the two adjacent detection chambers 23 is arranged between the two adjacent detection chambers 23, a clamping groove 22 with a downward opening is formed between the two adjacent detection sample guide heads 13, a collecting part 16 and the adjacent detection sample guide heads 13, and the upper parts of the first partition plates 27 are respectively used for being embedded in the corresponding clamping grooves 22.
Preferably, clamping grooves 22 with downward openings are arranged between two adjacent detection sample guide heads 13, between the collecting part 16 and the adjacent detection sample guide heads 13, the first partition plates 27 are in one-to-one correspondence with the clamping grooves 22 between the two adjacent detection sample guide heads 13, and the upper parts of the first partition plates 27 are respectively embedded in the corresponding clamping grooves 22; the side plate 25 of the detection pool far away from the high end of the bottom plate 19 can be embedded in the clamping groove 22 between the collecting part 16 and the adjacent detection sample guide head 13.
When in use, a reaction reagent is placed in the detection chamber 23 in advance, and the capillary 13 is inserted into the corresponding detection chamber 23, so that the detection pool is tightly connected below the sample guide groove body 21; taking a liquid sample to be measured, adding a sample from one end of the sample guide groove body 21, which is positioned at the high end of the bottom panel 19, into the sample guide groove body 21, enabling the liquid sample to flow from the high end to the low end along the inclined direction of the bottom panel 19, and filling the capillary tube 13 for quantitative sampling under the action of capillary phenomenon when the liquid sample sequentially flows through the first sample inlets 12; the residual liquid flowing down from the bottom plate 19 can enter the liquid collecting chamber 17 through the liquid inlet, and plays a role in collecting the residual liquid. Preferably, the liquid collecting chamber 17 is made of transparent material, in use, a reagent is placed in the liquid collecting chamber 17 in advance, and after the reagent reacts with the sample, spectrophotometry can be used to measure absorbance.
The rest of this embodiment is the same as embodiment 2, and will not be described here again.
The foregoing embodiments are preferred embodiments of the present invention, and in addition, the present invention may be implemented in other ways, and any obvious substitution is within the scope of the present invention without departing from the concept of the present invention.
Claims (6)
1. An apparatus for analysis and detection, characterized in that: the sample guide groove body comprises a plurality of side wall baffles and a bottom panel, wherein the side wall baffles are sequentially connected end to end, the bottom panel is obliquely arranged, the lower ends of the side wall baffles are connected with the bottom panel, the capillary tube is connected to the lower end of the bottom panel, the capillary tube is connected to the bottom panel, and the capillary tube and the collecting part are arranged from a high position end to a low position end along the oblique direction of the bottom panel; the lower end of the capillary tube is provided with a detection sample guide head, and a liquid outlet of the capillary tube is arranged on the detection sample guide head;
the bottom panel is provided with a first sample inlet penetrating through the bottom panel, and the liquid inlet of the capillary tube is communicated with the first sample inlet; the detection pool is detachably connected to the lower part of the capillary, the detection pool is formed by surrounding a bottom plate and a plurality of side plates, the number of the capillary is more than two, the capillary is sequentially arranged from a high position end to a low position end along the inclination direction of the bottom plate, the detection pool comprises a plurality of detection chambers for accommodating detection sample guide heads, a first partition plate for separating the two adjacent detection chambers is arranged between the two adjacent detection chambers, a clamping groove with a downward opening is arranged between the two adjacent detection sample guide heads, and the upper parts of the first partition plates are respectively used for being embedded in the corresponding clamping grooves;
or the bottom panel is provided with a first sample inlet and a second sample inlet which penetrate through the bottom panel, and the liquid inlet of the capillary tube is communicated with the first sample inlet; the collecting part is connected to the lower end of the bottom panel, the collecting part is provided with a diversion channel penetrating through the upper end of the collecting part and the lower end of the collecting part, and the second sample inlet is communicated with a liquid inlet of the diversion channel; the utility model provides a detection cell, including the capillary, the capillary is connected in the below of collecting part, the detection cell is enclosed by bottom plate and a plurality of side board and is established, the quantity of capillary is more than two, and a plurality of capillaries are arranged in proper order by high-order end to low-order end along the incline direction of bottom plate, the detection cell is including being used for holding the collecting chamber of establishing collecting part and a plurality of detection room that is used for holding the detection and leads the appearance head, is provided with the first baffle that is used for separating two adjacent detection rooms between two adjacent detection rooms, be provided with the second baffle that is used for separating collecting room and detection room between collecting room and the adjacent detection room, all be provided with the draw-in groove that the opening was down between two adjacent detection lead the appearance head, the upper portion of first baffle and the upper portion of second baffle are used for inlaying respectively to locate corresponding draw-in groove.
2. The apparatus for analysis and detection according to claim 1, wherein: the collecting part is connected to the low-level end of the bottom panel, the collecting part is provided with a liquid collecting chamber for collecting liquid, and the upper end of the liquid collecting chamber is provided with a liquid inlet.
3. The apparatus for analysis and detection according to claim 1, wherein: the collection chamber is made of a light transmissive material.
4. The apparatus for analysis and detection according to claim 1, wherein: the area of the bottom plate below the detection chamber is made of a light-transmitting material.
5. The apparatus for analysis and detection according to claim 1, wherein: the inner wall of the capillary tube is made of hydrophilic material.
6. A method for detecting cells or particles by using the device for analytical detection according to any one of claims 1 to 5, characterized in that: the method comprises the following steps:
(1) Connecting the capillary and the collection part with the detection chamber and the collection chamber respectively, wherein the collection chamber and the plurality of detection chambers are pre-provided with a reaction reagent;
(2) Taking a liquid sample, and adding the sample from one end of the sample guide groove body, which is positioned at the high end of the bottom panel, into the sample guide groove body;
(3) The liquid sample flows through the first sample inlet and then automatically fills the capillary tube, and the rest liquid sample completely enters the collecting chamber through the diversion channel and then reacts with the reagent in the collecting chamber; and (3) placing the device for analysis and detection in a centrifugal device for centrifugation, enabling the liquid sample in the capillary holes to flow into corresponding detection chambers and react with the reagents in the corresponding detection chambers respectively, and then adopting external detection and analysis equipment to analyze and detect the mixture after the reaction of the detection chambers and the collection chambers.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810404763.0A CN108362615B (en) | 2018-04-28 | 2018-04-28 | Device for analysis and detection and cell or particle detection method |
| PCT/CN2019/084534 WO2019206276A1 (en) | 2018-04-28 | 2019-04-26 | Apparatus for use in analysis and detection and cell or particle detection method |
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| CN201810404763.0A CN108362615B (en) | 2018-04-28 | 2018-04-28 | Device for analysis and detection and cell or particle detection method |
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| CN108362615B true CN108362615B (en) | 2023-06-27 |
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| WO2019206276A1 (en) * | 2018-04-28 | 2019-10-31 | 东莞德益生物医疗科技有限公司 | Apparatus for use in analysis and detection and cell or particle detection method |
| CN115993302B (en) * | 2023-03-22 | 2023-06-13 | 中铁工程服务有限公司 | Shield tail grease detection device and detection method |
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