CN103412130A - Reagent cup assembly, instrument and method for quickly determining content of specific proteins of urine - Google Patents
Reagent cup assembly, instrument and method for quickly determining content of specific proteins of urine Download PDFInfo
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- CN103412130A CN103412130A CN201310369264XA CN201310369264A CN103412130A CN 103412130 A CN103412130 A CN 103412130A CN 201310369264X A CN201310369264X A CN 201310369264XA CN 201310369264 A CN201310369264 A CN 201310369264A CN 103412130 A CN103412130 A CN 103412130A
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Abstract
The invention discloses a reagent cup assembly, instrument and method for quickly determining the content of specific proteins of urine. The reagent cup assembly is composed of a plurality of reagent cups which are connected, wherein a detection reagent is arranged at the bottom part in each reagent cup. The instrument comprises the reagent cup assembly, a sample adding device, a flow distributing device and a detector; the sample adding device is communicated with the flow distributing device by a pipeline; the detector is close to the reagent cup assembly and is internally provided with a plurality of detection systems; and the quantity of the detection systems is matched with that of the reagent cups in the reagent cup assembly. The method comprises the following steps of: (a) adding the detection reagent in the bottom parts of the reagent cups in the reagent cup assembly; (b) adding the same amount of urine to be determined in the reagent cups in (a); (c) uniformly mixing liquid in the reagent cups in (b); (d) carrying out hatching reaction on the liquid in (c); and (e) detecting the liquid in (d) by the detector. The reagent cup assembly, the instrument and the method disclosed by the invention have the advantages that the efficiency for detecting different specific proteins in the urine simultaneously can be improved and the detection result is accurate.
Description
Technical field
The invention belongs to biological detection instrument field, specifically, relate to reagent cup connection, instrument and the method for Fast Measurement urine specific protein content.
Background technology
In the process in prior art, the specific protein in urine detected, be all only to set a reagent cup, in testing process, successively in reagent cup, add respectively the urine determinand and detect reagent liquid, can only detect a kind of specific protein in urine at every turn.And if need to detect respectively a plurality of specific protein in urine the time, need to carry out repeated application of sample operation and the trace routine of start-up detector repeatedly, have a strong impact on detection efficiency.And add the time of urine determinand or liquid detecting reagent to spin out in reagent cup, degraded or rotten occurs in minute subconstiuent of some in urine determinand or liquid detecting reagent, be unfavorable for the complete reaction of urine determinand and detection reagent, and have a strong impact on the testing result accuracy.
Can be used at present detecting the instrument of Special Proteins in urine and mainly contain common Urine Analyzer, large automatic biochemical instruments or chemiluminescent analyzer.Common Urine Analyzer can only detect conventional physical and chemical index, metabolism class product carries out sxemiquantitative, and can not the specific albumen of quantitative measurement, as bladder chalone C, a1 microglobulin, b2 microglobulin, RBP ELISA, transferrins, microdose urine protein etc.Large automatic biochemical instruments or chemiluminescent analyzer are mainly used in measuring this a part of specific protein in blood, also can be used for measuring the part specific protein in urine, and because the material in blood and urine has difference in essence, in blood and urine, kind and the content difference of contained specific protein are also larger, after using full automatic biochemical apparatus or chemiluminescent analyzer to detect blood, must correct and the resetting of program by complicated parameter, or significantly cleaning could be used for the urine Protein Detection, and its detection sensitivity can't meet the requirement of urine protein determination.Full automatic biochemical apparatus or chemiluminescent analyzer cost are high in addition, and much the unit of check all can't bear the expense of buying expensive instrument, have greatly limited the usable range of full automatic biochemical apparatus or chemiluminescent analyzer.
Have again, Special Proteins kind in urine is many, no matter be common Urine Analyzer or large automatic biochemical instruments or chemiluminescent analyzer, in these instruments, only be provided with a set of detection system, therefore each detection can only detect a kind of specific albumen in urine, has had a strong impact on the detection efficiency in urine albumen.Many technician have pair instrument to improve, wish to use same instrument to detect simultaneously a plurality of specific proteins in urine, but because instrument or the setting of instrument internal parameter are not mated, need to carry out using same set of sample adding device to add in reagent cup before and after urine and tracer liquid, cause in testing process, liquid in each reagent cup pollutes mutually, had a strong impact on the accuracy that detects effect, in addition, in existing instrument, only be provided with a set of detection system, a set of detection system detects a plurality of samples successively, the trace routine that needs the corresponding number of times of operation, also had a strong impact on the detection efficiency in urine albumen.
Summary of the invention
Technical matters to be solved by this invention is to provide reagent cup connection, instrument and the method for Fast Measurement urine specific protein content.Use this reagent cup connection, instrument and method all can detect simultaneously the content of the different specific proteins in urine, improved detection efficiency, and testing result is accurate.
The present invention solves the problems of the technologies described above the technical scheme adopted:
The reagent cup connection of Fast Measurement urine specific protein content, connected and composed by a plurality of reagent cup, and described reagent cup inner bottom part has detection reagent.Can add simultaneously at different reagent cup inner bottom parts same to detect reagent according to the needs of detection or experiment purpose, also can add respectively simultaneously the detection reagent that complete difference or part are identical.Reagent cup inner bottom part in the reagent cup connection just is added with detection reagent before detection, can guarantee the urine determinand with detect reagent can complete reaction, avoided in testing process, adding again the detection reagent of liquid and caused liquid easily to spill the loss that reagent cup causes detection reagent, thereby having affected the accuracy of testing result.The setting of reagent cup connection, can, as urine determinand apparatus for placing, can be used as the reaction unit of urine determinand and detection reagent simultaneously.In to urine in the testing process of Special Proteins, a plurality of reagent cup in the reagent cup connection, can hold respectively simultaneously a plurality of urine determinands, after adding the urine determinand, the detection reagent of reagent cup inner bottom part just with urine determinand generation series reaction, the hazardous substance that formation can be identified by detecting device, by detecting device, can to the hazardous substance in a plurality of reagent cup, detect simultaneously, thereby realize once just detecting simultaneously the concentration of a plurality of specific proteins in urine, save the time, improved work efficiency.The setting of reagent cup connection, and the reagent cup inner bottom part detects the prior interpolation of reagent, can detect simultaneously a plurality of different specific proteins in the urine determinand, improved the detection efficiency of specific protein content and the accuracy of testing result in the urine significantly.
Described reagent cup is 2-8.Reagent cup in the reagent cup connection, can be according to the purpose needs that detect or test, the reagent cup of varying number is set, when the reagent cup in the reagent cup connection is more than or equal to 2, can detect simultaneously in urine and to cross a different types of Special Proteins, improve the efficiency of urine detection, but the reagent cup in reagent cup connection is while surpassing 8, the shared locus of reagent cup connection is excessive, also increased detecting device simultaneously to the detection difficulty of liquid in different reagent cup.
The side of adjacent reagent cup connects.The side of adjacent reagent cup is connected, and makes the shared space of reagent cup connection minimum, adds the urine determinand in the reagent cup in also being convenient to join to reagent cup simultaneously, is more conducive to detecting device the liquid in reagent cup is carried out to fast detecting.
All reagent cup basal surfaces are positioned at same plane.All reagent cup basal surfaces are positioned at same plane, make upper surface and the lower surface of each reagent cup all be positioned at a height, form orderly arrangement, add the urine determinand in the reagent cup in being convenient to join to reagent cup simultaneously, also be beneficial to detecting device the liquid in reagent cup is carried out to fast detecting.
The xsect of described reagent cup is the rectangular configuration of hollow.Be hollow in reagent cup, can receiving fluids, can be used as the reaction vessel of urine determinand and detection reagent, the xsect of reagent cup is rectangular configuration, can make reagent cup effectively utilize space, and can better carry out ordered arrangement between reagent cup, can better connect between reagent cup.
The instrument of Fast Measurement urine specific protein content, comprise that reagent cup connection, sample adding device, part flow arrangement and detecting device form, described sample adding device is communicated with part flow arrangement by pipeline, on part flow arrangement, be provided with the exit passageway that in joining with reagent cup, the quantity of reagent cup is complementary, each exit passageway extends to the top of reagent cup corresponding in the reagent cup connection by pipeline, detecting device is near the reagent cup connection, in detecting device, be provided with a plurality of detection systems, in the quantity of detection system and reagent cup connection, the quantity of reagent cup is complementary.Sample adding device is transported to part flow arrangement to the urine determinand by pipeline, the urine determinand adds in each reagent cup the reagent cup connection by pipeline respectively uniformly from each exit passageway on part flow arrangement, detection reagent in urine determinand mix reagent cup connection in reagent cup, the urine determinand with detect reagent after fully reacting, the associated thing that formation can be identified by detecting device, detecting device detects associated thing, thereby obtains the content of specific protein in the urine determinand.Part flow arrangement be provided with reagent cup connection in the exit passageway that is complementary of the quantity of reagent cup, part flow arrangement can be controlled the flow of urine determinand exit passageway, guarantees to each reagent cup, to add simultaneously the urine determinand of equivalent.The detection system arranged in detecting device, can to the Special Proteins thing of the urine determinand in reagent cup, measure accurately exactly, the quantity of detection system equates with the quantity of reagent cup in the reagent cup connection, formed the detection system with the reagent cup quantity Matching, a detection system detects the urine determinand in a reagent cup, can obtain testing result accurately, a plurality of detection systems are carried out the specific protein content detection to the liquid in all reagent cup in the reagent cup connection simultaneously, obtain simultaneously testing result separately, saved detection time, improved detection efficiency, avoid simultaneously using same detection system to detect successively the Special Proteins of urine in a plurality of reagent cup, and make detection time long, and easily cause the chaotic phenomenon of testing result intersection.
Described reagent cup connection is outside equipped with evenly mixing device, and evenly mixing device is provided with a plurality of puddlers, and in the quantity of puddler and reagent cup connection, the quantity of reagent cup is complementary.The mode mixed can be contactlessly mix, contact mixes, contactless blending manner easily is spread across the liquid in reagent cup outside reagent cup, causes the loss of liquid in reagent cup, and the liquid cross pollution in different reagent cup.Contact mix be provided with reagent cup connection in reagent cup quantity be complementary and corresponding puddler mutually respectively, can guarantee that a puddler stirs and evenly mixs the liquid in a reagent cup, in the situation that guarantee Blending Efficiency of Blending, effectively avoid in blending process the liquid cross pollution between the motion generation reagent cup of liquid.
The method of Fast Measurement urine specific protein content comprises the following steps: (a) the reagent cup inner bottom part in reagent cup connection add for detection of urine in the detection reagent of albumen; (b) in the reagent cup of (a), add the urine determinand of equivalent; (c) mix the liquid in (b) reagent cup; (d) liquid in (c) is carried out to incubation; (e) by detecting device, the liquid in the reagent cup in (d) is detected.The reagent cup connection is the reagent cup group be formed by connecting by a plurality of reagent cup, at the reagent cup inner bottom part, adds and detects reagent before detecting, and can avoid adding liquid detecting reagent in testing process.In the reagent cup connection, add equivalent urine determinand in each reagent cup, detection reagent in urine determinand while mix reagent cup, after the liquid that mixes reagent cup, urine determinand and detection reagent react in reagent cup, after reaching abundant reaction, the associated thing that formation can be identified by detecting device, detecting device detects the liquid in reagent cup, can obtain testing result accurately.
In sum, the invention has the beneficial effects as follows:
(1) reagent cup of Fast Measurement urine specific protein content of the present invention connection, the setting of reagent cup connection, and the reagent cup inner bottom part is added with detection reagent in advance, in testing process, reduce and add the step that detects reagent, just can detect simultaneously a plurality of different specific proteins in the urine determinand, improve significantly the detection efficiency of specific protein content and the accuracy of testing result in the urine.
(2) provide the instrument of Fast Measurement urine specific protein content, the detection system that in joining with reagent cup, the quantity of reagent cup is complementary is set in the detecting device in instrument, can to the liquid in all reagent cup in the reagent cup connection, detect simultaneously, obtain simultaneously testing result separately, save detection time, improved detection efficiency; Avoid simultaneously using same detection system to detect successively the Special Proteins of urine in a plurality of reagent cup, and make detection time long, and the phenomenon of cross pollution easily occurs.
(3) on instrument, arranging of part flow arrangement can shunt and control the urine determinand, make urine determinand energy while, equivalent evenly successfully add in each reagent cup, avoid the urine determinand spill reagent cup and cause damage, improved the detection rates of urine albumen.
(4) on instrument, be provided with reagent cup connection in the quantity of reagent cup be complementary and corresponding puddler mutually respectively, can guarantee that a puddler stirs and evenly mixs the liquid in a reagent cup, and avoid in blending process the liquid cross pollution between the motion generation reagent cup of liquid.
(5) provide the method for Fast Measurement urine specific protein content, the method is a plurality of different specific proteins in the fast detecting urine simultaneously, and testing result is accurate, and detection efficiency is high.
The accompanying drawing explanation
Fig. 1 is the structural representation of reagent cup connection of the present invention;
Fig. 2 is the structural representation of the instrument of Fast Measurement urine specific protein content of the present invention;
Fig. 3 is the diagrammatic cross-section of the instrument of Fast Measurement urine specific protein content of the present invention;
The structural representation of the instrument of Fig. 4 Fast Measurement urine of the present invention specific protein content;
Mark and corresponding parts title in accompanying drawing: 1-reagent cup; 2-reagent cup connection; 3-detection reagent; 4-part flow arrangement; 5-exit passageway; 6-sample adding device; 7-detecting device; 8-detection system; 9-evenly mixing device; 10-puddler; 11-syringe; 12-solenoid valve; 13-sample bottle.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited only to this.
Embodiment 1:
Referring to illustrated in figures 1 and 2, the reagent cup of the Fast Measurement urine specific protein content of the present embodiment connection, connected and composed by a plurality of reagent cup 1, and described reagent cup 1 inner bottom part is provided with the detection reagent 3 of coating structure.The setting of reagent cup connection 2, and reagent cup 1 inner bottom part detects the prior interpolation of reagent 3, can detect simultaneously a plurality of different specific proteins in the urine determinand, improved the detection efficiency of specific protein and the accuracy of testing result in the urine significantly.
Embodiment 2:
Referring to illustrated in figures 1 and 2, on the basis of embodiment 1, reagent cup described in the present embodiment 1 is 5.The side of adjacent reagent cup 1 connects.All reagent cup 1 basal surfaces are positioned at same plane.The xsect of described reagent cup 1 is the rectangular configuration of hollow.The side of adjacent reagent cup 1 is connected, and makes the shared space of reagent cup connection 2 minimum, adds the urine determinand in the reagent cup in also being convenient to join to reagent cup simultaneously, is more conducive to detecting device the liquid in reagent cup is carried out to fast detecting.All reagent cup basal surfaces are positioned at same plane, make upper surface and the lower surface of each reagent cup all be positioned at a height, form orderly arrangement, add the urine determinand in the reagent cup in being convenient to join to reagent cup simultaneously, also be beneficial to detecting device the liquid in reagent cup is carried out to fast detecting.Be hollow in reagent cup, can receiving fluids, can be used as the reaction vessel of urine determinand and detection reagent, the xsect of reagent cup is rectangular configuration, can make reagent cup effectively utilize space, and can better carry out ordered arrangement between reagent cup, can better connect between reagent cup.
Embodiment 3:
Extremely shown in Figure 3 referring to Fig. 1, the instrument of Fast Measurement urine specific protein content, comprise reagent cup connection 2, sample adding device 6, part flow arrangement 4 and detecting device 7 form, described sample adding device 6 is communicated with part flow arrangement 4 by pipeline, on part flow arrangement 4, be provided with reagent cup connection 2 in the exit passageway 5 that is complementary of the quantity of reagent cup 1, each exit passageway 5 extends to the top of reagent cup 1 corresponding in reagent cup connection 2 by pipeline, detecting device 7 is near reagent cup connection 2, in detecting device 7, be provided with a plurality of detection systems 8, in the quantity of detection system 8 and reagent cup connection 2, the quantity of reagent cup 1 is complementary.The detection system that in joining with reagent cup, the quantity of reagent cup is complementary is set in detecting device, can to the liquid in all reagent cup in the reagent cup connection, detects simultaneously, obtain simultaneously testing result separately, saved detection time, improved detection efficiency; Avoid simultaneously using same detection system to detect successively the Special Proteins of urine in a plurality of reagent cup, and make detection time long, and easily cause the chaotic phenomenon of testing result intersection.On instrument, arranging of part flow arrangement can shunt and control the urine determinand, and urine determinand energy while, equivalent are evenly added in each reagent cup smoothly, avoided the urine determinand spill reagent cup and cause damage.
Embodiment 4:
To shown in Figure 3, on the basis of embodiment 3, the connection of reagent cup described in the present embodiment 2 is outside equipped with evenly mixing device 9 referring to Fig. 1, and evenly mixing device 9 is provided with a plurality of puddlers 10, and in the quantity of puddler 10 and reagent cup connection 2, the quantity of reagent cup 1 is complementary.Evenly mixing device be provided with reagent cup connection in the quantity of reagent cup be complementary and corresponding puddler mutually respectively, can guarantee that a puddler stirs and evenly mixs the liquid in a reagent cup, and avoid in blending process the liquid cross-infection between the motion generation reagent cup of liquid.
Embodiment 5:
Referring to Fig. 4, the instrument of Fast Measurement urine specific protein content, comprise reagent cup connection 2, sample adding device 6, part flow arrangement 4 and detecting device 7 form, described sample adding device 6 is communicated with part flow arrangement 4 by pipeline, on part flow arrangement 4, be provided with reagent cup connection 2 in the exit passageway 5 that is complementary of the quantity of reagent cup 1, each exit passageway 5 extends to the top of reagent cup 1 corresponding in reagent cup connection 2 by pipeline, detecting device 7 is near reagent cup connection 2, in detecting device 7, be provided with a plurality of detection systems 8, in the quantity of detection system 8 and reagent cup connection 2, the quantity of reagent cup 1 is complementary.Sample adding device 6 in the present embodiment, by syringe 11, sample bottle 13 and solenoid valve 12, formed, syringe 11 and sample bottle 13 are by pipeline UNICOM, and solenoid valve 12 is arranged on the path between syringe 11 and sample bottle 13, and solenoid valve 12 is by pipeline and part flow arrangement 4 UNICOMs.Solenoid valve serves as switch control urine determinand and is passed to part flow arrangement.When the needs application of sample, first control solenoid valve, make syringe, solenoid valve and sample bottle be in conducting state, the swabbing action of syringe is the urine determinand in sample bottle, urine determinand in specimen bottle is drawn in the pipeline between syringe and solenoid valve, control solenoid valve again, make syringe, solenoid valve and part flow arrangement UNICOM, the liquid that syringe holder is in syringe and electromagnetic valve pipeline is shifted onto in part flow arrangement.The urine determinand adds respectively in each reagent cup contained in the reagent cup connection that detects reagent by shunt, the urine determinand with detect reagent and fully react and form associated thing after, with detecting device, associated thing is detected the content that just can obtain formulation Special Proteins in the urine determinand.
Embodiment 6:
The method of Fast Measurement urine specific protein content comprises the following steps: (a) the reagent cup inner bottom part in reagent cup connection add for detection of urine in the detection reagent of albumen; (b) in the reagent cup of (a), add the urine testing sample of equivalent, mix and detect reagent; (c) mix the liquid in (b) reagent cup; (d) liquid in (c) is carried out to incubation 1-3 minute; (e) by detecting device, the liquid in the reagent cup in (d) is detected.The method is a plurality of different specific protein content in the fast detecting urine simultaneously, and testing result is accurate, and detection efficiency is high.
Embodiment 7:
The reagent cup connected and composed by 5 reagent cup joins, at 5 reagent cup inner bottom parts, add respectively bladder chalone C and detect reagent, the a1 microglobulin detects reagent, the b2 microglobulin detects reagent, Retinal-binding protein detection reagent and transferrins detect reagent, by sample adding device, the urine determinand is added in part flow arrangement, the urine determinand is by 5 exit passageways of part flow arrangement, evenly add respectively simultaneously in urine determinand to 5 reagent cup of 0.5ml, detection reagent in urine determinand mix reagent cup, mix the liquid in reagent cup, the urine determinand fully reacted latter 3 minutes with detection reagent, the associated thing that formation can be identified by detecting device, with detecting device, associated thing is detected, can accurately detect respectively in 5 reagent cup the content of each contained specific protein in the urine determinand.
Embodiment 8:
The reagent cup consisted of 2 reagent cup joins, at 2 reagent cup inner bottom parts, add respectively transferrins and detect reagent, microdose urine protein detects reagent, by sample adding device, the urine determinand is added in part flow arrangement, , the urine determinand is by 2 exit passageways of part flow arrangement, evenly add respectively simultaneously in urine determinand to 2 reagent cup of 0.5ml, detection reagent in urine determinand mix reagent cup, mix the liquid in reagent cup, the urine determinand fully reacted latter 1 minute with detection reagent, the associated thing that formation can be identified by detecting device, with detecting device, associated thing is detected, can accurately detect respectively in 2 reagent cup the content of each contained specific protein in the urine determinand.
Claims (8)
1. the reagent cup of Fast Measurement urine specific protein content connection, is characterized in that, by a plurality of reagent cup (1), connected and composed, described reagent cup (1) inner bottom part has detection reagent (3).
2. the reagent cup of Fast Measurement urine specific protein content according to claim 1 connection, is characterized in that, described reagent cup (1) is 2-8.
3. the reagent cup of Fast Measurement urine specific protein content according to claim 1 connection, is characterized in that, the side of adjacent reagent cup (1) connects.
4. the reagent cup of Fast Measurement urine specific protein content according to claim 1 connection, is characterized in that, all reagent cup (1) basal surface is positioned at same plane.
5. the reagent cup of Fast Measurement urine specific protein content according to claim 1 connection, is characterized in that, the xsect of described reagent cup (1) is the rectangular configuration of hollow.
6. the instrument of Fast Measurement urine specific protein content, it is characterized in that, the reagent cup connection (2) that comprises claim 1 to 5 any one, sample adding device (6), part flow arrangement (4) and detecting device (7) form, described sample adding device (6) is communicated with part flow arrangement (4) by pipeline, on part flow arrangement (4), be provided with reagent cup connection (2) in the exit passageway (5) that is complementary of the quantity of reagent cup (1), each exit passageway (5) extends to the top of reagent cup (1) corresponding in reagent cup connection (2) by pipeline, detecting device (7) is near reagent cup connection (2), in detecting device (7), be provided with a plurality of detection systems (8), in the quantity of detection system (8) and reagent cup connection (2), the quantity of reagent cup (1) is complementary.
7. the instrument of Fast Measurement urine specific protein content according to claim 6, it is characterized in that, described reagent cup connection (2) is outside equipped with evenly mixing device (9), evenly mixing device (9) is provided with a plurality of puddlers (10), and in the quantity of puddler (10) and reagent cup connection (2), the quantity of reagent cup (1) is complementary.
8. the method for Fast Measurement urine specific protein content, is characterized in that, comprises the following steps: (a) the reagent cup inner bottom part in reagent cup connection add for detection of urine in the detection reagent of albumen; (b) in the reagent cup of (a), add the urine determinand of equivalent; (c) mix the liquid in (b) reagent cup; (d) liquid in (c) is carried out to incubation; (e) by detecting device, the liquid in the reagent cup in (d) is detected.
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Cited By (4)
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| CN104965066A (en) * | 2015-07-06 | 2015-10-07 | 山东大学 | Portable urine protein detection system based on mobile internet |
| CN105807040A (en) * | 2016-05-31 | 2016-07-27 | 四川金域医学检验中心有限公司 | Reagent device for biochemical index detection |
| CN108362615A (en) * | 2018-04-28 | 2018-08-03 | 东莞德益生物医疗科技有限公司 | A kind of analysis detection device and cell or particle detection technique |
| WO2019206276A1 (en) * | 2018-04-28 | 2019-10-31 | 东莞德益生物医疗科技有限公司 | Apparatus for use in analysis and detection and cell or particle detection method |
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Application publication date: 20131127 |