CN102707067A - Test strip for semi-quantitative detection of microalbuminuria - Google Patents
Test strip for semi-quantitative detection of microalbuminuria Download PDFInfo
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- CN102707067A CN102707067A CN2012101650741A CN201210165074A CN102707067A CN 102707067 A CN102707067 A CN 102707067A CN 2012101650741 A CN2012101650741 A CN 2012101650741A CN 201210165074 A CN201210165074 A CN 201210165074A CN 102707067 A CN102707067 A CN 102707067A
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Abstract
The invention discloses a test strip for semi-quantitative detection of microalbuminuria, belonging to medical test consumables. The test strip is characterized in that the test strip for semi-quantitative detection of microalbuminuria is a colloidal gold test strip. An anti-human albumin antibody and rabbit IgG with acolloidal gold label is enveloped on a gold label fiber pad; anti-rabbit IgG antibody is enveloped on a quality control line C, and human albumin is enveloped on a detection line T. The test strip is simple, sensitive and accurate, can be used for semi-quantitative detection of microalbuminuria in human urine and suitable for detection of early renal injury patients.
Description
Technical field,
The invention belongs to field of biological detection, relate to a kind of medical detection consumptive material, be specifically related to the test strips of semi-quantitative determination of urinary trace albumin.
Background technology
(Microalbuminuria MAU) is meant that albumin content exceeds the healthy subjects term of reference in the urine to microdose urine protein, but can not detect the variation of this trace with the method for routine.Microdose urine protein urine is meant and microalbumin in urine, occurs.Albumin is the normal protein matter in a kind of blood, but under physiological condition, only occurs the minute quantity albumin in the urine.Microalbuminuria reflection renal abnormality leaky protein.Microdose urine protein is the impaired sign of early stage kidney of diabetic nephropathy, hypertensive nephropathy etc.The microdose urine protein which kind of disease causes all is the intrinsic cells injury of kidney that causes because of initial reason difference, and the structure of the intrinsic cell of kidney is changed, and function changes with the variation of structure, the embodiment in urine.
In clinical, use the generation that urine trace of albumin index is monitored ephrosis usually.The detection of urine trace of albumin is the most responsive, the reliable diagnostic index of early detection ephrosis.Through the numerical value of microdose urine protein, just can diagnose the state of an illness comparatively accurately in conjunction with incidence, symptom and medical history statement.Judge that the state of an illness has got into which of fiberization in stage.So, regularly detecting microdose urine protein (U-MA), the ordinary people should be annual, and the patient who increases should test once in per 3 months.Like this, all played positive role for the prevention and the early stage treatment of ephrosis.When suffering from hypertension or diabetes or suffering from these two kinds of diseases (often taking place simultaneously) simultaneously, the function that pathology has changed kidney filtration protein (especially albumin) can take place in renal blood vessels, and this makes proteinaceous effluent in urine.Microalbuminuria is the early stage sign of diabetes affects kidney, is diabetic nephropathy.
Some domestic authoritys' ephrosis expert advocates that the early prevention microalbuminuria will begin from person between twenty and fifty now; Plentiful along with what live; Ephrosis is accompanied by more and more rejuvenations of complication such as diabetes, hypertension, heart disease, obesity, and it is the primary selection of people's daily life that a kind of sensitivity, easy, rapid semi-quantitative are measured the microdose urine protein test strips.
Microdose urine protein refers to be higher than normally; But the albuminuria that conventional method can't detect; His detection has received extensive attention as the important indicator of early stage renal damage diagnosis, and assay method comprises radioimmunology (RIA), ELISA (ELISA), immune rate scattering turbidimetry, immunoturbidimetry.Wherein highly sensitive, the high specificity of radioimmunology, precision height, accuracy good, the recovery nearly 100% (Xiong Lifan. clinical examination basis ［ M ］. the 3rd edition; Beijing: People's Health Publisher; 2004:132.); But its complex operation, and have problems such as radiation and pollution, basic hospital is difficult for carrying out; ELISA (ELISA) though detection limit lower (0.15mg/L), detection time is long, is not suitable for on-site measurement; Immunity rate scattering turbidimetry also has good clinical value (Cheng Suqin; Zhu Meicai. the value ［ J ］ of microdose urine protein in the early diagnosis of diabetes injury of kidney. Chinese laboratory medicine magazine; 2003; 7:740 741.), but these methods all can only be measured urinary albumin merely, stay the urine sample to limit if having time; Immunoturbidimetry is that fully-automatic equipment is realized its detection through IMMAGE immunochemistry system, and its accuracy is higher; But seeing that equipment and reagent cost an arm and a leg; Tachysynthesis turbidimetry shortcoming is that price is expensive, needs the professional to operate, and therefore all fails to popularize at basic hospital at present.
Application number is the method that 201110072901.8 patent of invention discloses microalbumin in a kind of high performance liquid chromatography fast measuring urine; This method velocity of separation has shortened detection time soon greatly; But its complicated operation; Need those skilled in the art to accomplish, and strong to device dependence, be not suitable for general basic hospital or family and detect.
Application number is that 200810073668.3 patent of invention discloses a kind of resonance scattering spectrometry that detects microdose urine protein, and this method is strong to device dependence, is not suitable for general basic hospital or family and detects.
Application number is that 200510066403.3 patent of invention discloses a kind of microdose urine protein testing chip and proving installation thereof, and this invention is convenient, directly perceived, fast, but it only can detect microdose urine protein qualitatively.
Above-mentioned instrument or method only can detect microdose urine protein qualitatively; A kind of colloid selenium test paper for semi-quantitative determination of urinary trace albumin is disclosed and application number is 200810305085.9 patent of invention; Fast, highly sensitive; High specificity, good stability, simple to operate, need not any instrument and equipment, the result judges intuitive and reliable, economical and practical.But whether it can only interpretation be normal level, is not sure of the concentration that exceeds normal level what are.
Immune colloidal gold technique (Immunogold labelling techique) is the solid phase labelling immunoassay that after luciferin, radioactive isotope and enzyme three big labelling techniques, grows up the eighties in last century.This technology has mainly utilized gold grain to have the characteristic of high electron density, when these labels are assembled at corresponding part place in a large number, forms macroscopic redness or pink spot, thereby is used for qualitative or semiquantitative tachysynthesis detection method.Fast diagnose test paper bar is a novel vitro diagnostic techniques that on monoclonal antibody technique, colloidal gold immunochromatographimethod technology and novel chromatographic material basis, has grown up since the nineties in 20th century; Development in recent years is rapid, has particularly obtained widespread use in the medical test at biomedical sector.This technology mainly is that specific antigen or antibody are fixed on the nitrocellulose membrane with ribbon; Colloid gold label reagent is adsorbed on the pad; Be added on the sample pad of test strips one end when testing sample after, move forward, react to each other behind the colloid gold label reagent on the dissolving pad through capillary action; When moving to fixing antigen or antibody regions again; Specificity takes place with it and combines and be trapped in determinand gold marked reagent compound, and colloid gold label accumulates in to detect and is with, can be through the range estimation result that developed the color intuitively.Free label is then crossed and is detected band, thereby reaches and detect the purpose that thing separates automatically.
Competition inhibition method can be used for measuring antigen or antibody; To measure antigen is example; Its principle is that the envelope antigen competition of being examined antigen and solid phase in the sample combines with labelled antibody; The amount of being examined antigen in sample is more than or equal to the threshold value of reagent, and then labelled antibody then can not combine with the envelope antigen of solid phase; Do not examined antigen if having in the sample, then labelled antibody directly combines with the envelope antigen of solid phase.
At present, the inhibition method is competed in immunity combine with the collaurum detection technique trace of albumin in human urine is carried out half-quantitative detection, and the colloidal gold strip with characteristics such as efficient, sensitive, special, convenient, that sensing range is wide rarely has report.
Summary of the invention
Be demand and the blank that solves above-mentioned field; The present invention aims to provide a kind of test strips of semi-quantitative determination of urinary trace albumin; Test strips of the present invention is competed immunity against the inhibition method and is combined with the collaurum detection technique microalbumin in the human urine is carried out half-quantitative detection, is used for kidney earlier damage patient's detection.
The test strips of semi-quantitative determination of urinary trace albumin comprises base plate supports layer, reaction reagent carrier absorption layer on short transverse, said reaction reagent carrier absorption layer is fixed on the said base plate supports layer;
Said reaction reagent carrier absorption layer is provided with the sample fiber pad from being stained with the liquid end to handheld terminal successively, gold mark fiber mat, nitrocellulose filter, handheld terminal absorbent material pad;
On said gold mark fiber mat, sample fiber pad and handheld terminal absorbent material pad, be coated with protective film; On said nitrocellulose filter, be provided with nature controlling line C, detection line T from handheld terminal successively to being stained with the liquid end, said nature controlling line C and detection line T are parallel to each other and are perpendicular with the test strips length direction;
It is characterized in that; The test strips of said semi-quantitative determination of urinary trace albumin is a colloidal gold strip; Be coated with the anti-human albumin antibody and the rabbit igg of colloid gold label on the said gold mark fiber mat, be coated with anti-rabbit igg on the said nature controlling line C and resist more, be coated with human albumin on the said detection line T.
Said detection line T is three, is parallel to each other, and sets gradually to being stained with the liquid end from handheld terminal, is respectively detection line T
1, T
2, T
3
Said anti-human albumin antibody is anti-human albumin monoclonal antibody.
Sample fiber pad protective film surface indicates the visual MAX markings parallel with said detection line.
Said sample fiber pad adopts glass fibre cotton, and said handheld terminal absorbent material pad adopts robust fibre thieving paper, and said gold mark fiber mat adopts the glass fibre cotton of ADSORPTION OF GOLD mark albumen.Absorbent material layer is not mentioned in the front
Said protective film is an adhesive tape.
The plastic base of said base plate supports layer for not absorbing water.
Also comprise shell; Said shell has null; The shape size of the simple and easy test strips that said null and said reaction reagent carrier absorption layer and said base plate supports layer form is complementary; Be used to load said reaction reagent carrier absorption layer, said upper surface of outer cover is respectively equipped with application of sample window and form corresponding to the position of sample fiber pad and nitrocellulose filter.
A kind of kit that is used for semi-quantitative determination of urinary trace albumin is characterized in that: can be used for packing aforesaid right requirement 1～8th, arbitrary test strips.
Technique effect:
Test strips of the present invention is simple to operate, and is easy to use, brings promptly and uses; Do not need one of skill in the art, can satisfy different levels personnel's needs, spread to hospital of grass-roots community and average family easily; With the symbol display result; Broken away from specific (special) requirements, can detect the microdose urine protein concentration of normal and improper level, be widely used in the half-quantitative detection of the microalbumin in the human urine equipment, instrument, personnel and storage and transportation environment; And have quick, easy, sensitive, accurate, special, the characteristics that sensing range is wide.
Microalbumin in the colloidal gold strip utilization competition inhibition method principle half-quantitative detection human urine of the present invention; Be the anti-human albumin monoclonal antibody of the albumin competition colloid gold label that encapsulates of albumin and T line in the urine, come the albumin in the half-quantitative detection human urine.Test strips of the present invention have from top to bottom four the colour developing bands, article one is nature controlling line (C), second and third, four (be respectively T for detection line
1, T
2, T
3).If 4 bar line all occurs, expression is normal, if T
1, T
2, T
3Disappear in succession, represent that then the degree of injury of kidney strengthens gradually.
Principle according to the collaurum detection; The mucus end of colloidal gold strip of the present invention is immersed in the liquid to be measured; Perhaps the sample fiber layer at the mucus end drips liquid to be measured; Target substance in the liquid to be measured moves to handheld terminal from being stained with the liquid end along test strips under the capillary action of fibrous sheet material, and target substance in the liquid to be measured adheres to each other with colloid gold label albumen during through gold mark fibrage, when the target substance concentration in the liquid to be measured is not enough to the albumen of the golden mark of complete association colloid; Remaining collaurum continue to move to detection line will with the material that encapsulates on the detection line because specificity combines to cause collaurum on detection line, to assemble; Make detection line colour developing, explanation at this moment, the concentration of target substance is lower than the concentration of target substance of the albumen representative of colloid gold label.On gold mark fibrage, no longer include the albumen of remaining colloid gold label; Can not accumulate on the detection line when then having combined albumen compound of the colloid gold label of target substance in the liquid to be measured to move to detection line; Therefore detection line can not develop the color; At this moment explanation, the concentration of the target substance in the liquid to be measured are higher than the concentration of the target substance of the binding ability representative of collaurum in the gold mark fibrage; At this moment; Further accurately confirm the concentration of target substance like need; Need through detecting again after liquid to be measured is diluted; Until detection line colour developing, the concentration that target substance in the liquid to be measured is described is marked between the concentration of the binding ability representative of collaurum in the fibrage with gold in the concentration that the concentration of detection line representative is multiplied by the extension rate gained, thereby has realized liquid to be measured is carried out half-quantitative detection.
Colloidal gold strip of the present invention is different from existing test strips part and is:
The test strips of semi-quantitative determination of urinary trace albumin of the present invention is a colloidal gold strip; Be coated with the anti-human albumin antibody and the rabbit igg of colloid gold label on the said gold mark fiber mat; How anti-be coated with anti-rabbit igg on the said nature controlling line C, is coated with human albumin on the said detection line T.Microalbumin in the test strips utilization of the present invention competition inhibition method principle half-quantitative detection human urine, promptly the albumin that encapsulates of the albumin in the urine and T line is competed the anti-human albumin antibody of colloid gold label, comes the albumin content in the half-quantitative detection human urine.
Said detection line T is three, is parallel to each other, and sets gradually to being stained with the liquid end from handheld terminal, is respectively detection line T
1, T
2, T
3According to three detection line T
1, T
2, T
3Difference colour developing situation, thereby realize half-quantitative detection to microdose urine protein.
The present invention; Be coated with anti-human albumin monoclonal antibody of having of colloid gold label and rabbit igg on the preferred said gold mark fiber mat, said anti-human albumin monoclonal antibody and rabbit igg respectively with detection line T on anti-rabbit igg resist of encapsulating on the human albumin that encapsulates and the nature controlling line C more carry out specific reactions.
The present invention preferably indicates the visual MAX markings parallel with said detection line T on sample fiber pad protective film surface.Be used to remind operating personnel, liquid to be measured does not surpass markings during operation, in order to avoid cause error.
The present invention, preferred said sample fiber pad adopts glass fibre cotton, and said absorbent material handheld terminal pad adopts robust fibre thieving paper, and said nitrocellulose filter adopts nitrocellulose filter, and said gold mark fiber mat adopts the glass fibre cotton of ADSORPTION OF GOLD mark albumen.These material fit are used, its water absorptivity and capillarity no-float, the detection speed and the accuracy of assurance test strip of the present invention.
The present invention, preferred protective film is an adhesive tape, makes test strips avoid polluting, cost is low and effective.
The present invention, the plastic base of preferable substrate supporting layer for not absorbing water, the material that does not absorb water, thus avoid propping material to absorb the migration that liquid to be measured influences horizontal direction.
The present invention, the lower surface of the upper surface of preferred sample fiber pad and said gold mark fiber mat is established layer of non-woven fabric.Be used for an amount of imbitition, play certain buffer action.
The present invention; Preferably also comprise shell; Said shell has null; The shape size of the simple and easy colloidal gold strip that said null and said reaction reagent carrier absorption layer and said base plate supports layer form is complementary, and is used to load said reaction reagent carrier absorption layer, and said upper surface of outer cover is respectively equipped with application of sample window and form corresponding to the position of sample fiber pad and nitrocellulose filter.Be easy to carry and preserve.
Microalbumin in colloidal gold strip utilization competition inhibition method principle of the present invention and the association colloid technology for gold half-quantitative detection human urine; Can detect the microdose urine protein concentration of normal and improper level; Be widely used in the half-quantitative detection of the microalbumin in the human urine, be used for kidney earlier damage patient's detection.Test strips of the present invention is simple to operate, and is easy to use, brings i.e. usefulness, do not need one of skill in the art, can satisfy different levels personnel's needs, and have quick, easy, sensitive, accurate, special, the characteristics that sensing range is wide.
Description of drawings:
The vertical view of Fig. 1 test strips of the present invention;
Wherein: 1-handheld terminal, 2-nature controlling line C line, 3-detection line T
1Line, 4-detection line T
2, 5-detection line T
3, 6-MAX markings, 7-are stained with the liquid end, 8-nitrocellulose filter, 9-sample fiber pad, 10-handheld terminal absorbent material pad, 11-gold mark fiber mat, 17-tape edge line
The longitudinal sectional drawing of Fig. 2 test strips of the present invention
Wherein: 8-nitrocellulose filter, 9-sample fiber pad, 10-handheld terminal absorbent material pad, 11-gold mark fiber mat, the big nonwoven fabrics of 12-, the little nonwoven fabrics of 13-, 14-handheld terminal adhesive tape, 15-arrow adhesive tape, 16-base plate supports layer.
Embodiment:
It is in order further to understand the present invention better that following embodiment is provided; Be not limited to said preferred forms; Content of the present invention and protection domain are not constituted restriction; Anyone makes up with the characteristic of other prior aries and the identical or akin product of any and the present invention that draws under enlightenment of the present invention or with the present invention, all drops within protection scope of the present invention.
The present invention aims to provide a kind of test strips of semi-quantitative determination of urinary trace albumin; Microalbumin in this test strips utilization competition inhibition method principle half-quantitative detection human urine; Be the anti-human albumin monoclonal antibody of the albumin competition colloid gold label that encapsulates of albumin and T line in the urine, come the albumin in the half-quantitative detection human urine.Test strips of the present invention have from top to bottom four the colour developing bands, article one is nature controlling line (C), second and third, four (be respectively T for detection line
1, T
2, T
3).If 4 bar line all occurs, expression is normal, if human albumin concentration raises gradually in the urine, and T so
1, T
2, T
3Will disappear in succession according to variable concentrations, then point out the degree of injury of kidney to strengthen gradually.Three red stripes are promptly arranged, i.e. C, T
2, T
3, explain that albuminous content is 20-50mg/L in the detection urine; Two red stripes are arranged, i.e. C, T
3, explain that albuminous content is 50-100mg/L in the detection urine; A red stripes is arranged, and promptly the C line explains that the albumin content that detects in the urine sample is greater than 100mg/L.Test strips of the present invention is simple to operate, and is easy to use, brings promptly and uses; Do not need one of skill in the art, can satisfy different levels personnel's needs, spread to hospital of grass-roots community easily; To the inspection of ephrosis early stage patient,, broken away from specific (special) requirements to equipment, instrument, personnel and storage and transportation environment with the symbol display result; Can be widely used in the half-quantitative detection of microdose urine protein, and have quick, easy, sensitive, accurate, special characteristics.
Principle according to the collaurum detection; The mucus end of test strips of the present invention is immersed in the liquid to be measured; Perhaps the sample fiber layer at the mucus end drips liquid to be measured; Target substance in the liquid to be measured moves to handheld terminal from being stained with the liquid end along test strips under the capillary action of fibrous sheet material, and target substance in the liquid to be measured adheres to each other with colloid gold label albumen during through gold mark fibrage, when the target substance concentration in the liquid to be measured is not enough to the albumen of the golden mark of complete association colloid; Remaining collaurum continue to move to detection line will with the material that encapsulates on the detection line because specificity combines to cause collaurum on detection line, to assemble; Make the colour developing of three detection lines, explanation at this moment, the concentration of target substance is lower than the concentration of target substance of the albumen representative of colloid gold label.Remaining collaurum concentration is different on gold mark fibrage, continues to move to detection line, owing to through detection line sequencing is arranged, first kind of T three kinds of situation can appear, in the combining of the albumen of the colloid gold label of limited concentration and detection line
2And T
3Colour developing, T
1Do not develop the color, pointing out liquid concentration to be measured is 20-50mg/L; Second kind of T
3Colour developing, T
1And T
2Do not develop the color, pointing out liquid concentration to be measured is 50-100mg/L; The third T
1, T
2, T
3Do not develop the color, point out liquid concentration＞100mg/L to be measured, explanation at this moment, the concentration of the target substance in the liquid to be measured is higher than the concentration of the target substance of the binding ability representative of collaurum in the gold mark fibrage; At this moment; Further confirm＞concentration of 100mg/L target substance like need; Need through detecting again after liquid to be measured is diluted; Until detection line colour developing, the concentration that target substance in the liquid to be measured is described is marked between the concentration of the binding ability representative of collaurum in the fibrage with gold in the concentration that the concentration of detection line representative is multiplied by the extension rate gained, thereby has realized liquid to be measured is carried out half-quantitative detection.
The test strips of semi-quantitative determination of urinary trace albumin comprises base plate supports layer 16, reaction reagent carrier absorption layer on short transverse, said reaction reagent carrier absorption layer is fixed on the said base plate supports layer 16; Said reaction reagent carrier absorption layer is provided with sample fiber pad 9 from being stained with liquid end 7 to handheld terminal 1 successively, gold mark fiber mat 11, nitrocellulose filter 8, handheld terminal absorbent material pad 10; On said gold mark fiber mat 11, sample fiber pad 9 and handheld terminal absorbent material pad 10, be coated with protective film.Be stained with liquid end 7 from handheld terminal to 1 on the said nitrocellulose filter 8 and be provided with nature controlling line C-2, detection line T (3,4,5) successively, said nature controlling line C-2, detection line T (3,4,5) are parallel to each other and perpendicular with said test strips length direction.
Colloidal gold strip of the present invention is different from existing test strips part and is:
The test strips of said semi-quantitative determination of urinary trace albumin is a colloidal gold strip; Be coated with the anti-human albumin antibody and the rabbit igg of colloid gold label on the said gold mark fiber mat 11, be coated with anti-rabbit igg on the said nature controlling line C-2 and resist said detection line T (3 more; 4,5) be coated with human albumin on.Microalbumin in the test strips utilization competition inhibition method principle half-quantitative detection human urine of the present invention; Be albumin and the T (3 in the urine; 4,5) the anti-human albumin antibody of the albumin competition colloid gold label that encapsulates of line comes the albumin in the half-quantitative detection human urine.
Said detection line T is three, is parallel to each other, and sets gradually to being stained with liquid end 7 from handheld terminal 1, is respectively detection line T
1-3, T
2-4, T
3-5.According to three detection line T
1-3, T
2-4, T
3-5 difference colour developing situation, thus realize half-quantitative detection to microdose urine protein.
The present invention; Preferably; Said anti-human albumin antibody is anti-human albumin monoclonal antibody, is coated with anti-human albumin monoclonal antibody of having of colloid gold label and rabbit igg on the said gold mark fiber mat 11, said anti-human albumin monoclonal antibody and rabbit igg respectively with detection line T (3; 4,5) the anti-rabbit igg that encapsulates on human albumin that encapsulates on and the nature controlling line C-2 resists more is carried out specific reaction.
The present invention preferably indicates the visual MAX markings 6 parallel with said detection line T (3,4,5) on sample fiber pad 9 protective film surfaces.Be used to remind operating personnel, liquid to be measured does not surpass MAX markings 6 during operation, in order to avoid cause error.
The present invention, preferred said sample fiber pad 9 adopts glass fibre cotton, and said handheld terminal absorbent material pad 10 adopts robust fibre thieving paper, and said gold mark fiber mat 11 adopts the glass fibre cotton of ADSORPTION OF GOLD mark albumen.These material fit are used, its water absorptivity and capillarity no-float, the detection speed and the accuracy of assurance test strip of the present invention.
The present invention, preferred protective film is an adhesive tape, makes test strips avoid polluting, cost is low and effective.
The present invention, the plastic base of preferable substrate supporting layer 16 for not absorbing water, the material that does not absorb water, thus avoid propping material to absorb the migration that liquid to be measured influences horizontal direction.
The present invention, the lower surface of the upper surface of preferred sample fiber pad 9 and said gold mark fiber mat 11 is established layer of non-woven fabric.Be used for an amount of imbitition, play certain buffer action, guarantee the detection speed and the accuracy of test strip of the present invention.
The present invention; Preferably also comprise shell; Said shell has null; The shape size of the simple and easy colloidal gold strip that said null and said reaction reagent carrier absorption layer and said base plate supports layer form is complementary, and is used to load said reaction reagent carrier absorption layer, and said upper surface of outer cover is respectively equipped with application of sample window and form corresponding to the position of sample fiber pad and nitrocellulose filter.Be easy to carry and preserve.
The preparation method of experimental example 1. test strips of the present invention
The biologically active raw material: anti-human albumin monoclonal antibody, rabbit igg, how anti-purchase source with human albumin is Canadian Artron BioResearch Inc. import to anti-rabbit igg
Reagent: bovine serum albumin(BSA) (BSA), casein (casein) are available from Sigma.
Nitrocellulose filter: German Sai Duolisi (Sartorius) company
The preparation procedure of test strips is following
Step 1. preparation coating buffer
Resist with 20mM PBS rabbit igg resists and human albumin dilutes more, will resist rabbit igg to resist more and be mixed with 2mg/ml, human albumin is mixed with 0.3mg/ml, is respectively applied for encapsulating of C on the nitrocellulose filter, T line.
Encapsulating of step 2. reagent
Encapsulate anti-rabbit igg on the nitrocellulose membrane resists and human albumin more:
Encapsulate respectively in T line and C line with the machine of encapsulating human albumin and anti-rabbit igg be how anti-, the spacing between C, the T line is 4.0mm, and C, T line width are 0.8mm, and the speed of encapsulating is 40mm/s.Be 6 hours drying time, and temperature is 30 ℃, humidity≤35%.
The preparation of the anti-human albumin monoclonal antibody of step 3. colloid gold label
Get the colloidal gold solution of 40nm, press final concentration 8-12 μ l/ml and add 0.2M K
2CO
3, add anti-human albumin monoclonal antibody and be prepared into certain concentration, placed room temperature reaction 15-30 minute, press the solution that final concentration 20 μ l/ml add 20%BSA then, be used for saturated free collaurum.
Centrifugal 30 minutes of 10000rpm removes unconjugated protein in the supernatant.Redissolve the colloid gold label stoste of deposition with the 0.5M BAS (containing 1%Tween, 5% sucrose, 1% hydrolysis BSA, 1% caseinhydrolysate, 0.1%PVA) of 1/10 volume.Working solution adopts above-mentioned 0.5m BAS dilution, and concentration is the 10-20% of stoste.
The preparation of the rabbit igg of step 4. colloid gold label
Get the colloidal gold solution of 40nm, press final concentration 8-12 μ l/ml and add 0.2M K
2CO
3, add rabbit igg and be prepared into certain concentration, placed room temperature reaction 15-30 minute, press the solution that final concentration 20 μ l/ml add 20%BSA then, be used for saturated free collaurum.
Centrifugal 30 minutes of 10000rpm removes unconjugated protein in the supernatant.Redissolve the colloid gold label stoste of deposition with the 0.5M BAS (containing 1%Tween, 5% sucrose, 1% hydrolysis BSA, 1% caseinhydrolysate, 0.1%PVA) of 1/10 volume.Working solution adopts above-mentioned 0.5m BAS dilution, and concentration is 6% of stoste.
Step 5 gold is marked fibrolaminar mark:
The anti-human albumin monoclonal antibody solution of colloid gold label and the rabbit igg of colloid gold label are mixed by above concentration, be laid on the 30*30cm fiberglass packing, liquid volume added is that 35ml/ opens, and be 6 hours drying time, and temperature is 30 ℃, humidity≤35%.
The assembling of step 6. test strips:
1, plastic plate is lain on the operator's console, stick double sticky tape.
2, tear the double sticky tape lining paper, sticking to be coated with anti-rabbit igg resists and the nitrocellulose filter of human albumin more apart from 30mm place, plastic plate upper end.
3, the robust fibre letterweight nitrocellulose filter upper end 2mm that absorbs water is sticked, even up on upper end and the plastic plate.
4, press 0.5mm place, nitrocellulose filter lower end to stick little nonwoven fabrics.
5, gold mark pad is evened up be pressed on above the little nonwoven fabrics.
6, fiberglass packing is pressed on gold mark pad and goes up half thely approximately, stick at 2mm place, following end distance plastic plate lower end.
7, big nonwoven fabrics and gold mark pad upper end are evened up, lower end and plastic plate lower end are evened up and are sticked.
8, press nitrocellulose filter 1.5-2mm to stick on the arrow adhesive tape.
9, press robust fibre thieving paper to stick on the handheld terminal adhesive tape.
Embodiment 2. method of application and methodology contrast experiment
This product method of application:
Get on the sample fiber pad that urine to be measured is added drop-wise to horizontal test strips, or the mucus end MAX line of test strips is immersed about 5 seconds in the urine with the lower part, take out horizontal positioned, sentence read result in 5 minutes.
Contrast 1: product contrast
The product of the embodiment of the invention 1 is compared with the product of prior art detection microdose urine protein to have fast; Accurately, special, characteristics easily; Clean and maintenance work in the forward and backward necessary apparatus of need not doing of detection microdose urine protein, and do not require that one of skill in the art operates.
Reference product: the microalbumin of Luo Shi diagnostic products (Shanghai) Co., Ltd. is measured No. the 2402465th, kit (immunoturbidimetry) state food medicine prison tool (advancing) word 2011
The result is following: result and immunoturbidimetry detection by quantitative reagent data basically identical can be used as the sxemiquantitative product and use.And advantage is fast, and is accurately convenient, need not professional equipment and professional, than immunoturbidimetry better application in basic hospital and family.
Correlation data such as table 1:
| Pilot project | Reference product | Product of the present invention (T2) |
| 0mg/L internal control sample | ＜20mg/L | ＜20mg/L |
| 10mg/L internal control sample | ＜20mg/L | ＜20mg/L |
| 20mg/L internal control sample | 20.2mg/L | About 20mg/L |
| 50mg/L internal control sample | 51.6mg/L | About 50mg/L |
| 100mg/L internal control sample | 102.5mg/L | About 100mg/L |
| Positive urine 20 examples | 20/20 | 20/20 |
| Negative urine 20 examples | 20/20 | 20/20 |
Contrast 2: methodology contrast
The embodiment of the invention 1 with market on other products that detect microdose urine proteins compare; Need not any instrument, can the concrete numerical value of interpretation, and guarantee the accuracy and the sensitivity that detect; It is quick; Accurately, convenient, inexpensive characteristics make it can spread to the monitoring that family and basic hospital are used for injury of kidney fast.
1) though the product of the triumphant wound in Shanghai also is a colloidal gold method; But can only be qualitative to judging whether the microalbumin content in the human urine exceeds standard; Be not sure of concrete numerical value, and what in interpretation as a result, adopt is colourimetry, the result is difficult for interpretation; Owing to the error of naked eyes, can cause the result of many mistakes.
2) the microalbumin test strips (dry chemical method) of Siemens's medical diagnostic prods (Shanghai) Co., Ltd.; No. the 2400522nd, state's food medicine prison tool (advancing) word 2012; The method is to adopt the method for dry chemical to come judged result; A little less than its sensitivity, early stage injury of kidney patient's albumin level be can not judge, clinical early diagnosis and prevention are unfavorable for.
3) microalbumin of Luo Shi diagnostic products (Shanghai) Co., Ltd. is measured No. the 2402465th, kit (immunoturbidimetry) state food medicine prison tool (advancing) word 2011; The method is to adopt immunoturbidimetry; Be through IMMAGE immunochemistry system; Fully-automatic equipment is realized its detection, and its accuracy is higher, still all fails to popularize at basic hospital at present in view of apparatus expensive.
Correlation data such as table 2:
Embodiment 3
The embodiment of the invention 1 with application number be that 200810305085.9 patent of invention discloses a kind of colloid selenium test paper for semi-quantitative determination of urinary trace albumin; Whether compare can interpretation be normal level not only; And can also confirm to exceed the concentration of normal level, sensing range is wide.
Correlation data such as table 3:
Interpretation of result: colloidal gold method detect test paper can half-quantitative detection go out 20mg/L, 50mg/L, 100mg/L,＞the concrete numerical value of 100mg/L; And colloid selenium test paper can only interpretation whether be normal level (＜20mg/L); Or abnormal level (>=20mg/L); Actually qualitative product should be classified as, the half-quantitative detection test paper can not be called.
Embodiment 4
The embodiment of the invention 1 compare correlation data such as table 4 with Kaichuang Biotechnology Co. Ltd., Shanghai product: the lot number B201108044 of Kaichuang Biotechnology Co. Ltd., Shanghai
Interpretation of result:
Triumphant wound product adopts the colourimetry sentence read result; T line colourity is judged to negative findings greater than the C line; T line colourity equals the C line and is judged to threshold value, and T line colourity does not go out to be judged to positive findings less than C line or T line, can not distinguish positive concrete numerical value; And the method sentence read result naked eyes error of colorimetric is bigger, makes result's accuracy receive certain influence.
Colloidal gold semi-quantitative of the present invention through three detection lines fade away judge concrete numerical value: 20mg/L, 50mg/L, 100mg/L,＞100mg/L; Relatively can be easy to judge the degree of damage of injury of kidney; And C, the more triumphant wound of T line colourity is dark, interpretation easily on naked eyes.
Embodiment 5
The professional uses test strips precision of the present invention to compare correlation data such as table 5 with the layman:
| Negative 20 examples | Positive 20 examples | The success of the test rate | Detect the time spent | |
| The layman | 20/20 | 20/20 | 100% | 15 minutes |
| The professional | 20/20 | 20/20 | 100% | 13 minutes |
Find through contrast; The layman can easier grasp the usage of test strips, to positive and negative result's accuracy of judgement, does not have the situation of failure; And there is not basic gap on detection time; Can be used for individual or basic hospital fully and use, not need training, directly just can manipulate with reference to instructions.
Microalbumin in test strips utilization competition inhibition method principle of the present invention and the association colloid technology for gold half-quantitative detection human urine is used for kidney earlier damage patient's detection.Test strips of the present invention is simple to operate, and is easy to use, brings i.e. usefulness, do not need one of skill in the art, can satisfy different levels personnel's needs, and guarantees sensitivity, accuracy and the high-level efficiency of detection.
Claims (9)
1. the test strips of semi-quantitative determination of urinary trace albumin comprises base plate supports layer, reaction reagent carrier absorption layer on short transverse, and said reaction reagent carrier absorption layer is fixed on the said base plate supports layer;
Said reaction reagent carrier absorption layer is provided with the sample fiber pad from being stained with the liquid end to handheld terminal successively, gold mark fiber mat, nitrocellulose filter, handheld terminal absorbent material pad;
On said gold mark fiber mat, sample fiber pad and handheld terminal absorbent material pad, be coated with protective film; On said nitrocellulose filter, be provided with nature controlling line C, detection line T from handheld terminal successively to being stained with the liquid end, said nature controlling line C and detection line T are parallel to each other and are perpendicular with the test strips length direction;
It is characterized in that; The test strips of said semi-quantitative determination of urinary trace albumin is a colloidal gold strip; Be coated with the anti-human albumin antibody and the rabbit igg of colloid gold label on the said gold mark fiber mat, be coated with anti-rabbit igg on the said nature controlling line C and resist more, be coated with human albumin on the said detection line T.
2. according to the said test strips of claim 1, it is characterized in that said detection line T is three, be parallel to each other, set gradually to being stained with the liquid end, be respectively detection line T from handheld terminal
1, T
2, T
3
3. according to the said test strips of claim 1, it is characterized in that said anti-human albumin antibody is anti-human albumin monoclonal antibody.
4. according to the said test strips of claim 1, it is characterized in that sample fiber pad protective film surface indicates the visual MAX markings parallel with said detection line.
5. according to the said test strips of claim 1, it is characterized in that said sample fiber pad adopts glass fibre cotton, said handheld terminal absorbent material pad adopts robust fibre thieving paper, and said gold mark fiber mat adopts the glass fibre cotton of ADSORPTION OF GOLD mark albumen.
6. according to the said test strips of claim 1, it is characterized in that said protective film is an adhesive tape.
7. according to the said test strips of claim 1, it is characterized in that the plastic base of said base plate supports layer for not absorbing water.
8. according to the arbitrary said test strips of claim 1-7; It is characterized in that; Also comprise shell, said shell has null, and the shape size of the simple and easy test strips that said null and said reaction reagent carrier absorption layer and said base plate supports layer form is complementary; Be used to load said reaction reagent carrier absorption layer, said upper surface of outer cover is respectively equipped with application of sample window and form corresponding to the position of sample fiber pad and nitrocellulose filter.
9. a kit that is used for semi-quantitative determination of urinary trace albumin is characterized in that: can be used for packing aforesaid right requirement 1～8 arbitrary test strips.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101158683A (en) * | 2007-05-16 | 2008-04-09 | 山东省农业科学院植物保护研究所 | Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof |
| CN101339193A (en) * | 2008-08-28 | 2009-01-07 | 河南省农业科学院 | Eperythrozoonosis rapid diagnosis test paper |
| CN101408545A (en) * | 2008-11-12 | 2009-04-15 | 河南省农业科学院 | Melamine fast detecting test paper bar and test paper card |
| CN101539578A (en) * | 2009-02-17 | 2009-09-23 | 李红玉 | Colloidal gold test strip for testing melamine content |
| CN101566636A (en) * | 2008-04-24 | 2009-10-28 | 北京科美东雅生物技术有限公司 | Magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and preparation method thereof |
| CN101871938A (en) * | 2010-05-12 | 2010-10-27 | 蓝十字生物药业（北京）有限公司 | Rapid test strip with colloid gold label |
| CN102062777A (en) * | 2009-11-12 | 2011-05-18 | 上海科新生物技术股份有限公司 | Hepatopathy detecting test paper by colloidal gold chromatography and preparation method thereof |
-
2012
- 2012-05-24 CN CN2012101650741A patent/CN102707067A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101158683A (en) * | 2007-05-16 | 2008-04-09 | 山东省农业科学院植物保护研究所 | Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof |
| CN101566636A (en) * | 2008-04-24 | 2009-10-28 | 北京科美东雅生物技术有限公司 | Magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and preparation method thereof |
| CN101339193A (en) * | 2008-08-28 | 2009-01-07 | 河南省农业科学院 | Eperythrozoonosis rapid diagnosis test paper |
| CN101408545A (en) * | 2008-11-12 | 2009-04-15 | 河南省农业科学院 | Melamine fast detecting test paper bar and test paper card |
| CN101539578A (en) * | 2009-02-17 | 2009-09-23 | 李红玉 | Colloidal gold test strip for testing melamine content |
| CN102062777A (en) * | 2009-11-12 | 2011-05-18 | 上海科新生物技术股份有限公司 | Hepatopathy detecting test paper by colloidal gold chromatography and preparation method thereof |
| CN101871938A (en) * | 2010-05-12 | 2010-10-27 | 蓝十字生物药业（北京）有限公司 | Rapid test strip with colloid gold label |
Non-Patent Citations (7)
| Title |
|---|
| JOUNG-HWAN,ET AL.: "Semiquantitative,bar code version of immunochromatographic assay system for human serum albumin as model analyte", 《BIOTECHNOLOGY AND BIOENGINEERING》 * |
| KOBRA OMIDFAR，ET AL.: "Development of a colloidal gold-based immunochromatographic test strip for screening of microalbuminuria", 《HYBRIDOMA》 * |
| 储迅涛 等: "免疫胶体金定量测定尿微量白蛋白的研究", 《标记免疫分析与临床》 * |
| 卢仁泉 等: "尿微量白蛋白半定量金免疫层析试条的研制和临床评价", 《检验医学》 * |
| 曹丹如: "快速检测尿微量白蛋白的半定量金免疫渗滤法的研究", 《检验医学》 * |
| 朱文钏 等: "免疫胶体金技术的应用及展望", 《生物技术通报》 * |
| 沈建雄 等: "新的尿微量白蛋白测定试剂的研制及评估", 《中国医药导刊》 * |
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