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CN104535768A - Urinary microalbumin detection apparatus - Google Patents

Urinary microalbumin detection apparatus Download PDF

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Publication number
CN104535768A
CN104535768A CN 201410692826 CN201410692826A CN104535768A CN 104535768 A CN104535768 A CN 104535768A CN 201410692826 CN201410692826 CN 201410692826 CN 201410692826 A CN201410692826 A CN 201410692826A CN 104535768 A CN104535768 A CN 104535768A
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detection
apparatus
microalbumin
sample
urinary
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CN 201410692826
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Chinese (zh)
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CN104535768B (en )
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马莉
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东莞市青麦田数码科技有限公司
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere

Abstract

A disclosed urinary microalbumin detection apparatus comprises a box holder, a box cover and a reagent strip; the box cover is provided with a sample adding hole and an observation port; the reagent strip comprises a support base sheet and a chromatography structure disposed on the base sheet; the chromatography structure comprises sample filtering paper, an immune colloidal gold paper sheet, an immune nitrocellulose membrane and water adsorption paper which are in successive head-to-tail connection arrangement; and the apparatus is characterized in that the sample adding hole also is provided with an urine cup opened at the bottom, and the bottom opening of the urine cup has the shape and the size both same to those of the sample adding hole, the immune nitrocellulose membrane is provided with a quality control band and multiple detection lines, and the quality control band and the multiple detection lines are exposed in the observation port. The urinary microalbumin detection apparatus has the advantages of convenient operation, rapid reaction, high sensitivity, high specificity, economy and utility, uneasiness to contaminate hands, capability of quantificationally obtaining the concentration interval of urinary microalbumin, and the like, and is suitable for on-site detection and personal usage.

Description

一种尿微量白蛋白检测装置 Means one kind of urinary microalbumin

技术领域 FIELD

[0001] 本发明涉及医学临床诊断技术领域,特别涉及一种采用免疫胶体金快速检测技术制备的定量尿微量白蛋白检测装置。 [0001] The present invention relates to medical technical field of clinical diagnosis, and particularly to a quantitative urinary albumin miniprep techniques immunogold detection means for rapid detection.

背景技术 Background technique

[0002] 微量白蛋白是指尿中的白蛋白含量超出了健康人的参考范围,在尿中非正常的少量存在,排泄量大于30mg/天或20mg/L,它通常被认为是肾功能衰竭、糖尿病和心血管疾病并发症的重要早期临床标志。 [0002] refers to albumin albumin content in the urine of healthy individuals outside the reference range, in the presence of a small amount of urine not normally excreted greater than 30mg / day or 20mg / L, it is generally considered renal failure , an important early clinical signs of diabetes and cardiovascular disease complications. 因此,尿液中微量白蛋白的检测对疾病的早期诊断、早期治疗和减小风险有重要的参考价值和临床意义。 Therefore, urine albumin testing has important reference value and clinical significance of early diagnosis, early treatment and reduce the risk of disease.

[0003] 现有的尿微量白蛋白的检测方式为:用尿杯采取尿液,然后用滴管吸取一定体积的尿液滴到尿微量白蛋白检测试纸的加样垫上,然后进行显色分析。 [0003] The conventional detection system for microalbuminuria: taking urine cup with urine, followed by pipetting a volume of the urine was dropped urinary albumin loading pad test strip, followed by chromogenic assay . 采用该方法,需要额外准备尿杯、滴管,增加了采样成本,且尿杯一般把较短,采样时易污染手,尿液转移过程中还存在尿液洒落的问题。 Using this method, you need to prepare additional urine cup, dropper, increasing the cost of sampling, and easily contaminated urine cup generally the shorter sampling time hand, the urine transfer process, there are still problems of urine spilled.

发明内容 SUMMARY

[0004] 为了解决上述问题,同时准确获知尿微量白蛋白的浓度范围,本发明提供了一种定量尿微量白蛋白快速检测的装置,其具有定量检测的能力,操作简单、反应快速、经济实用,不需要额外的配件即可实现实时的测量,特别适合个人自测。 [0004] In order to solve the above problems, while accurately informed of urinary albumin concentration range, the present invention provides a rapid quantitative microalbuminuria detection apparatus, which has the ability quantitative detection, simple operation, fast response, economical and practical It does not require additional accessories to achieve real-time measurement, especially for personal self-test.

[0005] 本发明提供的尿微量白蛋白检测装置,包括盒座、盒盖和试剂条,所述盒盖上设有加样孔与观察口,所述试剂条包括支撑基片及位于所述支撑基片上的层析结构,该层析结构包括依次头尾相接排列的滤样纸、免疫胶体金纸片、免疫硝酸纤维素膜和吸水纸,其特征在于:所述加样孔上还设置有底部开口的尿杯,该尿杯的底部开口与所述加样孔形状、大小相同,所述免疫硝酸纤维素膜上设置有质控带及多条检测线,所述质控带和多条所述检测线暴露在所述观察口中。 [0005] microalbuminuria detection apparatus according to the present invention provides, comprises a cassette holder, the lid and reagent strip, said loading aperture and is provided with a viewing port on the lid, the reagent strip comprises a supporting substrate and located chromatography on a supporting substrate structure, which structure comprises chromatographed sequentially arranged end to end like filter paper, immunogold paper, cellulose nitrate membranes and immune absorbent paper, characterized in that: said further wells plus urine cup is provided with a bottom opening, a bottom opening of the urine cup with the loading hole shape, the same size, the cellulose nitrate film, immune and control line is provided with a plurality of detecting lines, and said control line exposing the plurality of detecting lines in the mouth was observed.

[0006] 优选的,所述尿杯与盒盖一体成型。 [0006] Preferably, the lid is integrally formed with a urine cup.

[0007] 优选的,所述尿杯底部具有可向外翻的边缘,该边缘固定在所述盒体和所述盒座之间。 [0007] Preferably, the bottom of the urine cup having outwardly upturned edge, which is fixed between the cassette holder and the cassette.

[0008] 优选的,所述尿杯远离所述层析结构的一侧还设置有一开口,所述开口的最低端离所述加样孔的高度H为样品检测最少用量/加样孔的面积。 [0008] Preferably, the side away from the chromatographic urine cup structure is also provided with an opening, the opening height H from the lowest end of the loading aperture to detect a minimum amount of sample / loading area of ​​the hole .

[0009] 优选的,所述开口与一导管连接,所述导管另一端开口低于所述盒座。 [0009] Preferably, the opening is connected to a conduit, the conduit opening is lower than the other end of the cartridge holder.

[0010] 优选的,所述盒盖与所述盒座的边缘相匹配且相互粘合,或者所述盒盖与所述盒座上设有对应的卡合或扣合用连接部件,并经所述连接部件相卡合或扣合。 [0010] Preferably, the edge of the lid matches the cassette holder and bonded to each other, and the cartridge cover or the base is provided with corresponding engaging or snap connecting member in combination, and by the said connecting member is engaged or snap.

[0011] 优选的,所述免疫胶体金纸片上包被有胶体金标记;所述质控带上包被有抗兔IgG多抗,所述检测线上包被有人白蛋白。 [0011] Preferably, the colloidal gold immunoassay paper sheet is coated with a colloidal gold labeled; quality control of the tape is coated with a polyclonal anti-rabbit IgG, the test line was coated with albumin.

[0012] 优选的,所述检测线为3条,其上包被的人白蛋白的含量逐倍增加,所述检测线对应的所述盒盖上具有相应的浓度区间的标识。 [0012] Preferably, the detection line 3 on which the coated amount of human albumin by fold increase, with an identification of the corresponding density range the detection line of the corresponding cover.

[0013] 所述检测装置的检测方法为:在排尿时手捏吸水纸一端,直接将尿液尿在尿杯中,尿液流入加样孔,多余的尿液顺着尿杯一侧的开口排出。 [0013] The method for detecting apparatus for detecting: at one end of the absorbent paper pinching urination, the urine in the urine the urine directly to the cup, the urine flows into the sample hole, the excess urine opening along one side of the urine cup discharge. 然后将检测装置平放,计时,3-8分钟内判读结果,根据检测线的条数确定样品的浓度区间。 The detecting means is then laid flat, timing, interpretation of the results within 3-8 minutes, determined in accordance with the concentration of the sample interval the number of detection lines.

[0014] 本发明的尿微量白蛋白检测装置具有操作简便、反应快速、敏感性高、特异性强、经济实用、不易脏手、可定量获得尿微量白蛋白的浓度区间等优点,适合现场检测和个人使用。 [0014] microalbuminuria detection apparatus according to the present invention is simple, fast response, high sensitivity, specificity, economical, easily dirty, microalbuminuria obtain quantitative concentration range and suitable for field testing and personal use.

附图说明 BRIEF DESCRIPTION

[0015] 图1为本发明的尿微量白蛋白检测装置结构示意图; MAlb apparatus configuration [0015] FIG. 1 is a schematic view of the present disclosure;

[0016] 图2为本发明的尿微量白蛋白检测装置结构爆炸图; [0016] microalbuminuria detection apparatus of FIG. 2 an exploded view of the structure of the present invention;

[0017] 图3为本发明实施例1的盒盖剖视图; [0017] FIG. 3 a sectional view of a cover of the embodiment of the present invention;

[0018] 图4(a)_(h)为本发明实施例1试条的显色情况; [0018] FIG. 4 (a) _ (h) of the present embodiment where the color of a test strip embodiment of the invention;

[0019] 图5为本发明实施例2的盒盖剖视图。 [0019] Figure 5 a sectional view of the cover of the embodiment of Example 2 of the present invention.

具体实施方式 detailed description

[0020] 现结合具体实施例对本发明进行详细的说明。 [0020] Now with reference to specific embodiments of the present invention will be described in detail.

[0021] 实施例1 [0021] Example 1

[0022] 如图1、图2和图3所示,本发明的尿微量白蛋白检测装置,包括盒座1、盒盖2和试剂条3,盒盖2上设有加样孔21与观察口22,试剂条3包括支撑基片31及位于支撑基片31上的层析结构,该层析结构包括依次头尾相接排列的滤样纸32、免疫胶体金纸片33、免疫硝酸纤维素膜34和吸水纸35。 [0022] As shown in FIG 1, FIG, microalbuminuria detection means 2 and 3, the present invention includes a cassette holder, the cassette cover 2, and the test strip 3, observed with sample hole 21 and the upper cover 2 port 22, the test strip 3 comprises a substrate 31 and a support structure positioned on the chromatography support substrate 31, the structure comprises a chromatography successively arranged end to end like filter paper 32, the paper sheet 33 immunogold immune nitrocellulose fibroin film 34 and absorbent paper 35. 在加样孔21上设置有底部开口的尿杯23,尿杯23与盒盖2 一体成型。 On the loading hole 21 is provided with an opening 23 in the bottom of the urine cup, urine cup 23 integrally formed with the cover 2. 该尿杯23的底部开口与加样孔21形状、大小相同,在免疫硝酸纤维素膜34上设置有质控带344及第一检测线341、第二检测线342、第三检测线343,质控带344和第一检测线341、第二检测线342、第三检测线343暴露在观察口22中。 The bottom of the opening 23 of the urine cup shape of the loading aperture 21, the same size, provided immunologically nitrocellulose membrane 34 has a first control line 344 and the detection line 341, a second detection line 342, third line 343 is detected, detecting a first control line 344 and line 341, a second detection line 342, the third detected line 343 exposed in the viewing port 22. 在尿杯23远离层析结构的一侧还设置有一开口24,开口24的最低端离加样孔21的高度H为样品检测最少用量/加样孔21的面积。 Chromatography on one side of the structure remote from the urine cup 23 is also provided with an opening 24, the lowermost end of the opening amount of the sample from the detected minimum height H of the loading aperture 21 of the 24/21 of the area of ​​the sample hole. 开口24与一导管25连接,另一端开口26低于盒座1,优选地,导管25从试剂条3的孔36和盒座11上的孔穿出。 Opening 24 is connected to a conduit 25, the other end opening of the cassette holder 26 is less than 1, preferably, the catheter 25 out through the hole 3 of the reagent strip 11, holes 36 and the cassette holder. 采用这样的设计,可以使尿杯23中的尿液体积保持在低于尿杯开口24水平位置,多余的尿量将顺着与开口24连接的导管25流走,以防洒落、脏手。 With this design, it allows the volume of urine 23 in the urine cup holding position below the level of urine cup opening 24, excess urine will flow down along the conduit 25 connected to the opening 24 to prevent spills, dirty hands. 盒盖2与盒座I的边缘相匹配且相互粘合,或者盒盖2与盒座I上设有对应的卡合或扣合用连接部件,并经连接部件相卡合或扣合。 The edge of the cassette holder 2 and the lid match and I bonded to each other, or the cassette holder is provided with a cover 2 on the corresponding I in combination or snap engagement connection member, via the connection member and is engaged or snap. 免疫胶体金纸片32上包被有胶体金标记;质控带344上包被有抗兔IgG多抗,第一检测线341、第二检测线342、第三检测线343上包被有人白蛋白,人白蛋白的含量逐倍增加。 Immunogold paper sheet 32 ​​is coated with the colloidal gold labeled; control line 344 coated with anti-rabbit IgG polyclonal antibody, detection of the first line 341, second line 342 is detected, the detection line 343 on the third was coated with white protein, human albumin content of by-fold increase. 设置第一检测线341、第二检测线342、第三检测线343的浓度拐点分别为20mg/mL、50mg/mL、100mg/mL。 Detecting a first line 341, second line 342 is detected, the concentration of the third inflection point detection line 343 were 20mg / mL, 50mg / mL, 100mg / mL. 在第一检测线341、第二检测线342、第三检测线343对应的盒盖I上设置相应的浓度区间的标识。 In the first detection line 341, second line 342 is detected, identify the appropriate settings on the concentration of the third section 343 corresponding to the cover detection line I. 当尿液中的白蛋白呈阴性即小于20mg/mL时,质控带344和第一检测线341、第二检测线342、第三检测线343共四条显色带都显色,如图4(a)所示;当尿液中的白蛋白含量为20-50mg/mL时,质控带344和第一检测线341、第二检测线342显色,如图4(b)所示;当尿液浓度为50-100mg/mL时,质控带344和第一检测线341显色,如图4(c)所示;当尿液中白蛋白的含量大于100mg/mL时,仅质控带344显色,如图4 (d)所示。 When the albumin in the urine was negative, i.e. less than 20mg / mL, a first control line 344 and the detection line 341, a second detection line 342, a third detection line 343 are a total of four color ribbons significant, 4 (a) shows; when the urine albumin content is 20-50mg / mL, the first control line 344 and the detection line 341, the second detected line color 342, FIG. 4 (b); when the urine concentration of 50-100mg / mL, a first control line 344 and a color detection line 341, FIG. 4 (c) below; albumin in the urine when the content is more than 100mg / mL, only qualitative color control strip 344, FIG. 4 (d) shown in FIG. 为了实现0-20mg/mL、20-50mg/mL、50-100mg/mL及大于100mg/mL的人白蛋白的浓度范围检测,包被在硝酸纤维素膜各检测线上的比例约为2:3:50 To achieve 0-20mg / mL, 20-50mg / mL, 50-100mg / mL and greater than 100mg / mL of human albumin concentration range is detected, the proportion of nitrocellulose coated film of each test line 2 is approximately: 3:50

[0023] 该检测装置的检测方法为:在排尿时手捏吸水纸35 —端,直接将尿液尿在尿杯23中,尿液流入加样孔21,多余的尿液顺着尿杯23—侧的开口24排出。 [0023] The detection method of the detection device is: in urination pinching absorbent paper 35 - end of the urine in the urine the urine directly to the cup 23, the urine flows into the sample hole 21, the excess urine urine cup 23 along - a discharge opening 24 side. 然后将检测装置平放,尿液经过滤样纸32在层析作用下向吸水纸35 —端流到,达到免疫胶体金纸片33,当待检测尿样中不含尿白蛋白或白蛋白浓度小于20mg/mL,尿液中的抗原与少量金标抗体发生反应,没有结合抗原的金标抗原再层析到第一检测线341、第二检测线342、第三检测线343位置时,固定在硝酸纤维素膜34上的抗原会捕捉金标抗体,故第一检测线341、第二检测线342、第三检测线343都显色,质控线344也显色,此时得到的是阴性结果,表示白蛋白含量属于正常范围,如图4(a)所示;当尿样中含有的尿白蛋白含量大于lOOmg/mL时,尿样中的白蛋白与金标抗体完全结合,形成金标抗原抗体复合物,在沿硝酸纤维素膜34层析到第一检测线341位置时,金标抗体已没有空余的位点与第一检测线341上的人白蛋白结合,故第一检测线341不显色,同理,第二检测线342、第三 The detecting means is then laid flat, the filtered urine sample to the absorbent paper sheet 3235 under the action of chromatography - end flows, the paper sheet 33 reaches immune colloidal gold, the urine sample to be detected when the free urinary albumin or albumin gold antigen concentration of less than 20mg / mL, with a small amount of urine gold-labeled antigen-antibody reaction, the antigen is not bound to a first detection rechromatographed line 341, second line 342 is detected, when the third detected line position 343, antigen immobilized on nitrocellulose film 34 captures the gold-labeled antibody, so that the first detection line 341, a second detection line 342, third line 343 are detected color, the color quality control line 344 is also, at this time to give the a negative result indicates the content of the albumin within the normal range, as shown in Figure 4 (a); urinary albumin when urine contained greater than lOOmg / mL, urine albumin and gold labeled antibody binding completely, a gold labeled antigen-antibody complexes, when a cellulose nitrate film 34 along the detection line 341 to the first chromatographic position, no gold-labeled antibody has binding sites of spare human albumin on the first detection line 341, so that the first detecting a color line 341 is not the same token, the second detected line 342, the third 测线343也不显色,而只质控线344显色,如图4(d)所示。 Sensing line 343 is not a color, but only the color quality control line 344, FIG. 4 (d) shown in FIG. 此检测结果表明微量白蛋白的含量高,应及时去医院医治;当尿液中的白蛋白含量为50-100mg/mL时,尿液中的白蛋白与金标抗体结合后,再沿硝酸纤维素膜34层析到第一检测线341,金标抗体还有空余的位点与第一条检测线341上的人白蛋白结合,故第一检测线341显色,但金标抗体没有空余的位点与第二检测线342、第三检测线343上的人白蛋白结合,故第二检测线342、第三检测线343不显色,而质控线344显色,如图4(c)所示,此检测结果说明尿液中含有的蛋白较高,应引起足够的重视并及时去医院检查;当尿液中的白蛋白的含量为20-50mg/mL时,尿液中的白蛋白与金标抗体结合后,金标抗体有空余的位点与第一检测线341、第二检测线342上的白蛋白结合,从而使第一检测线341、第二检测线342都显色,第三条检测线343不显色,质控线344显色,如图4(b)所示,此检测结果说 This result indicates that the detection of high albumin content, should go to hospital for treatment; when the urine albumin content of 50-100mg / mL, with urine albumin binding the gold labeled antibody, and then along the nitrocellulose chromatography fibroin film 34 to the first detection line 341, there are people in the spare bits from the first sense lines 341 gold labeled albumin binding antibodies, the detection line 341 so that a first color, but no free gold-labeled antibody the site and the second detection line 342, a third person on the albumin binding detection line 343, so that a second detection line 342, line 343 does not detect the third color, the color quality control line 344, FIG. 4 ( c), the detection result indicating a higher protein contained in urine, and sufficient attention should go to hospital; when the urine albumin content is 20-50mg / mL, urine after the gold-labeled antibody binding with albumin, the gold-labeled antibody with a spare bit line 341 from the first detector, a second detection line on the albumin binding 342, so that the first detection line 341, second line 342 are significantly detected color, line 343 does not detect the third color, the color quality control line 344, FIG. 4 (b), the said detection result 明尿样中含有一定量的白蛋白,属于微量白蛋白尿的早期,即肾脏损伤病变的早期,如果初次发现应及时去医院做进一步的检查。 Ming contain a certain amount of urine albumin, it belongs to the early microalbuminuria, that lesions of early kidney damage, if the initial discovery should go to hospital for further examination. 如果质控线344不显色,无论第一检测线341、第二检测线342、第三条检测线343是否显色,如图4 (e)、图4 (f)、图4 (g)、图4 (h)所示,都表示检测装置失效,检测结果不可信。 If no color control line 344, either the first detected line 341, a second detection line 342, third line 343 detects whether the color, FIG. 4 (e), FIG. 4 (f), FIG. 4 (g) , FIG. 4 (h), the detecting means have said failure detection result credible. 实施例2 Example 2

[0024] 本实施例为对实施例1的微小变形,其整体结构和检测带均相同,唯一的区别在于,尿杯23不与盒盖2 —体成型而是采用以下结构:尿杯23底部具有可向外翻的边缘27,该边缘27固定在盒体I和盒座2之间。 [0024] This embodiment is a slight modification of the embodiment in Example 1, and the overall structure of the detection zone are the same, the only difference is that the cup 23 does not cover the urine 2 - shaping but the following structure: bottom urine cup 23 having between 27, the edge 27 is fixed in the cartridge and the cartridge holder 2 may be I outwardly turned edge.

[0025] 本发明的尿微量白蛋白检测装置可以快速、准确地检测尿微量白蛋白的不同浓度范围,不但可以用于早期肾脏损伤病变的预防和定期检查,而且可以用于微量白蛋白尿治疗过程中的监测,且使用方便,不脏手。 [0025] microalbuminuria detection apparatus according to the present invention can quickly and accurately detect microalbuminuria different concentration ranges, not only can be used for prevention and early lesions in kidney damage regular inspection, but also for the treatment of microalbuminuria process monitoring, and easy to use, no dirty hands.

Claims (8)

  1. 1.一种尿微量白蛋白检测装置,包括盒座、盒盖和试剂条,所述盒盖上设有加样孔与观察口,所述试剂条包括支撑基片及位于所述支撑基片上的层析结构,该层析结构包括依次头尾相接排列的滤样纸、免疫胶体金纸片、免疫硝酸纤维素膜和吸水纸,其特征在于:所述加样孔上还设置有底部开口的尿杯,该尿杯的底部开口与所述加样孔形状、大小相同,所述免疫硝酸纤维素膜上设置有质控带及多条检测线,所述质控带和多条所述检测线暴露在所述观察口中。 A urinary microalbumin apparatus comprising a cassette holder, the lid and reagent strip, said loading aperture and is provided with a viewing port on the lid, the reagent strip comprises a supporting substrate and a substrate on said support chromatography of the structure, the structure comprising sequentially chromatographed arranged end to end like filter paper, immunogold paper, cellulose nitrate membranes and immune absorbent paper, characterized in that: there is further provided on said bottom loading aperture urine cup opening, a bottom opening of the urine cup with the loading hole shape, the same size, the cellulose nitrate film, immune and control line is provided with a plurality of detecting lines, and a plurality of said control line is said detection line exposed at the mouth was observed.
  2. 2.根据权利要求1所述的尿微量白蛋白检测装置,其特征在于,所述尿杯与盒盖一体成型。 2. microalbuminuria detection apparatus according to claim 1, wherein said cover is integrally molded with a urine cup.
  3. 3.根据权利要求1所述的尿微量白蛋白检测装置,其特征在于:所述尿杯底部具有可向外翻的边缘,该边缘固定在所述盒体和所述盒座之间。 3. microalbuminuria detection apparatus according to claim 1, wherein: said urine cup having a bottom outwardly upturned edge, which is fixed between the cassette holder and the cassette.
  4. 4.根据权利要求1-3任意一项所述的尿微量白蛋白检测装置,其特征在于:所述尿杯远离所述层析结构的一侧还设置有一开口,所述开口的最低端离所述加样孔的高度H为样品检测最少用量/加样孔的面积。 The urinary albumin detection apparatus according to any one of claims 1-3, wherein: said urine cup away from the side structure further chromatography provided with an opening, from the lowermost end of the opening the height H of the loading aperture detecting a minimum amount of sample / area plus the sample aperture.
  5. 5.根据权利要求4所述的尿微量白蛋白检测装置,其特征在于:所述开口与一导管连接,所述导管另一端开口低于所述盒座。 5. mAlb apparatus as claimed in claim 4, wherein: said opening is connected to a conduit, the conduit opening is lower than the other end of the cartridge holder.
  6. 6.根据权利要求1所述的微量尿白蛋白检测装置,其特征在于,所述盒盖与所述盒座的边缘相匹配且相互粘合,或者所述盒盖与所述盒座上设有对应的卡合或扣合用连接部件,并经所述连接部件相卡合或扣合。 The albumin microalbuminuria detection apparatus according to claim 1, characterized in that the cover edge of the cassette holder and bonded to each other to match, or the cover is provided with the cartridge base a corresponding engagement or snap connecting member in combination, and through said connector member is engaged or snap.
  7. 7.根据权利要求1所述的尿微量白蛋白检测装置,其特征在于:所述免疫胶体金纸片上包被有胶体金标记;所述质控带上包被有抗兔IgG多抗,所述检测线上包被有人白蛋白。 The urinary albumin detection apparatus according to claim 1, wherein: said package sheet immunogold marker is colloidal gold; quality control of the tape is coated with a polyclonal anti-rabbit IgG, the said test line was coated with albumin.
  8. 8.根据权利要求7所述的尿微量白蛋白检测装置,其特征在于:所述检测线为3条,其上包被的人白蛋白的含量逐倍增加,所述检测线对应的所述盒盖上具有相应的浓度区间的标识。 8. microalbuminuria detection apparatus according to claim 7, wherein: said detection line 3, which coated by content of human albumin fold increase, corresponding to the detection line identify the corresponding concentrations with the lid section.
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