CN113777009A - Cell analyzer suitable for sheep, goats and cows and cell detection method - Google Patents
Cell analyzer suitable for sheep, goats and cows and cell detection method Download PDFInfo
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Abstract
The invention is applicable to the technical field of sample analysis and discloses a cell analyzer and a cell detection method applicable to sheep, goats and cows. The cell analyzer comprises an analyzer execution main body, a human-computer interaction device and a controller; the controller is configured to: when the animal detection mode selected by the man-machine interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute a blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample liquid, controlling the analyzer execution main body to perform leucocyte counting detection on the first detection sample liquid by adopting an impedance method, wherein the first type animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode, and the first preset proportion value is larger than or equal to 1200:1 and less than or equal to 3500: 1. the invention realizes the accurate detection of the parameters of the white blood cells of the blood sample of the sheep or goat or cow by adopting an impedance method.
Description
Technical Field
The invention relates to the technical field of sample analysis, in particular to a cell analyzer and a cell detection method suitable for sheep, goats and cows.
Background
The cell analyzer provided by the traditional technology adopts an expensive optical detection device to detect the blood sample leukocyte parameters of animals, can be suitable for detecting the blood sample leukocyte parameters of dogs, cats, horses, sheep, goats and cows, and has high accuracy of detection results. However, since the cell analyzer employs an optical detection device with a complicated structure and a high cost, it is not suitable for popularization and application in small and medium medical places and laboratories.
In order to solve the above technical problems, related technical personnel propose a scheme for detecting leukocyte parameters of animal blood samples by using an impedance detection channel, and the specific implementation manner of the scheme is as follows: diluting the blood sample to 250-500 degrees by using a diluent and a hemolytic agent in a leukocyte counting cell to prepare a detection sample solution, and then carrying out leukocyte counting detection on the detection sample solution in the leukocyte counting cell by using an impedance method through a detection device. When the testing method is used for detecting the leukocyte parameters of the blood samples of the dogs, cats and horses, more accurate detection results can be obtained, and the cost of the cell analyzer for detecting the dogs, cats and horses can be greatly reduced. However, when the testing method is used for detecting the parameters of the blood sample leukocytes of the sheep, the goat and the cow, the accuracy of the detection result is very poor, and the requirement of the blood sample leukocyte detection of the sheep, the goat and the cow cannot be met. Therefore, in the prior art, a cell analyzer with a high-cost optical detection device is basically adopted to detect the white blood cell parameters of blood samples of sheep, goats and cows.
Disclosure of Invention
The invention aims to provide a cell analyzer suitable for sheep, goats and cows, and aims to solve the technical problem of high cost of the cell analyzer for detecting white blood cells of sheep, goats and cows in the prior art.
In order to achieve the purpose, the invention provides the following scheme: a cell analyzer suitable for sheep, goats and cows comprises an analyzer execution main body, a human-computer interaction device and a controller;
the analyzer execution body is configured to be capable of performing a white blood cell count detection item on a blood sample using an impedance method;
the human-computer interaction device is configured to be able to select an animal detection mode as follows: the detection device comprises a first type animal detection mode and a second type animal detection mode, wherein the first type animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
controlling the analyzer execution main body to execute the following actions on the blood sample according to the animal detection mode selected by the man-machine interaction device:
when the animal detection mode selected by the human-computer interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute a blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample solution, and controlling the analyzer execution main body to perform leucocyte counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data of leucocytes, wherein the first detection data comprises a first counting value of the leucocytes;
when the animal detection mode selected by the human-computer interaction device is a second animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a second preset proportion value through diluent and hemolytic agent to prepare a second detection sample solution, and controlling the analyzer execution main body to perform leucocyte counting detection on the second detection sample solution by adopting an impedance method;
wherein the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500:1, the second preset proportion value is greater than or equal to 100: 1 and less than or equal to 500: 1.
the second purpose of the invention is to provide a cell analyzer suitable for sheep, goats and cows, which comprises an analyzer execution main body, a human-computer interaction device and a controller;
the analyzer execution body is configured to be capable of performing a white blood cell count detection item on a blood sample using an impedance method;
the human-computer interaction device is configured to be capable of selecting a first type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
controlling the analyzer execution main body to perform the following actions on the blood sample according to the animal detection mode selected by the human-computer interaction device:
when the animal detection mode selected by the human-computer interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample solution, and controlling the analyzer execution main body to perform leucocyte counting detection on the first detection sample solution by adopting an impedance method;
wherein the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500: 1.
the third purpose of the invention is to provide a cell detection method suitable for sheep, goats and cows, which comprises the following steps:
a sampling step, controlling a sampling device to collect a blood sample in a blood sample container;
a first sampling step of controlling the sampling device to transfer at least a part of the collected blood sample to the leucocyte counting cell;
a first detection sample solution preparation step of controlling a diluent supply device to convey diluent to the white blood cell counting cell, and controlling a hemolytic agent supply device to convey hemolytic agent to the white blood cell counting cell to dissolve red blood cells in the blood sample, and diluting the blood sample to a first preset proportion value to prepare a first detection sample solution;
a first detection step of controlling a first detection device to perform white blood cell counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of the white blood cells;
wherein the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500: 1.
the cell analyzer and the cell detection method suitable for sheep, goats and cows provided by the invention dilute the blood sample of the sheep or goat or cow to be more than or equal to 1200:1 and less than or equal to 3500:1 (the dilution ratio is far greater than that of cat, dog and horse blood samples detected by adopting an impedance method), preparing a first detection sample liquid, and then carrying out white blood cell counting detection on the first detection sample liquid by adopting the impedance method, so that a more accurate white blood cell count value of the blood samples of sheep, goats and cows can be obtained, and the effect of accurately detecting the white blood cell count value of the sheep, goats and cows by adopting the impedance method is realized. Because the cost of the impedance detection device for impedance counting detection is far lower than that of the optical detection device for optical counting detection, the cost of the cell analyzer for detecting the white blood cells of sheep, goats and cows can be greatly reduced, and the invention is favorable for large-scale popularization and application in small and medium medical places and laboratories.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic diagram of the composition of a cell analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the components of an analyzer implementation body provided by an embodiment of the present invention;
FIG. 3 is a schematic diagram of the pulse variation of a cell passing through a detection well according to an embodiment of the present invention;
FIG. 4 is a second histogram provided by an embodiment of the present invention;
FIG. 5 is a first schematic diagram provided by an embodiment of the present invention;
FIG. 6 is a third histogram provided by an embodiment of the present invention;
fig. 7 is a histogram of sheep white blood cells detected in horse detection mode according to an embodiment of the present invention.
The reference numbers illustrate:
100. an analyzer execution body; 110. a leukocyte counting pool; 111. a detection hole; 112. a first chamber; 113. a second chamber; 120. a first detection device; 121. a constant current source; 122. an electrode; 130. a negative pressure device; 140. a sampling device; 200. a human-computer interaction device; 300. and a controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, and the like in a specific posture, and if the specific posture is changed, the directional indicator is changed accordingly.
It will also be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or be indirectly connected to the other element through intervening elements.
In addition, the descriptions related to "first", "second", etc. in the present invention are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
Prior to the present application, a cell analyzer for measuring white blood cell count parameters of animals by impedance method was basically only used for testing animals such as cats, dogs, horses; when the method is used for detecting the white blood cell count of sheep, goats and cows, the detected value is very different from the actual value. Therefore, prior to this application, white blood cell count detection in sheep, goats, and cows was essentially performed by a cell analyzer having an expensive optical detection device.
The research of implementing sheep, goat and cow leucocyte count detection based on impedance method by those skilled in the art focuses on the technical route of improving hemolytic agent, but has not achieved significant effect. The inventor of the application breaks through the conventional thinking in the research of realizing the white blood cell counting detection of sheep, goats and cows based on the impedance method, focuses on the technical route of greatly changing the dilution ratio of the blood sample, and provides the effect of accurately detecting the white blood cell parameters of the sheep, goats and cows by adopting the impedance counting detection method with high dilution ratio (far higher than the dilution ratio of cats, dogs and horses) to count the white blood cells of the sheep, goats and cows.
Prior to this application, those skilled in the art would not have thought of substantially changing the blood sample dilution ratio, which could improve the white blood cell count measurements of sheep, goats and cows, because:
1) before the application, in a cell analyzer for detecting animal leukocyte counting parameters by adopting an impedance method, the blood sample dilution ratio of each animal is basically the same and is set between 250 and 500, and if the dilution ratio is lower than 250, the linearity of leukocytes is influenced, so that the accuracy of a high-value leukocyte (a large number of leukocytes) test result is influenced; whereas, if the dilution ratio is greater than 500, the accuracy of the test result of low-value white blood cells (with a small number of white blood cells) is affected, and the repeatability of the test result is affected (the results of multiple tests are very different).
2) In addition, the applicant found in the course of the study: in the white blood cell impedance counting detection of animals such as cats, dogs, horses and the like, a relatively accurate white blood cell counting value can be obtained by setting the dilution ratio between 250 and 500, and if the dilution ratio is increased to 800, 1200, 2400 or even 3000, the counting value is not obviously changed.
3) And the applicant also found in the research process that: in the white blood cell impedance counting detection of animals such as sheep, goats and cows, if the dilution ratio is selected to be in the range of being the same as or close to (between 250-500) the animals such as cats, dogs and horses, the volume of red blood cell fragments formed after red blood cells are broken is obviously larger after the hemolytic agent is added and is close to the volume of lymphocytes, so that the volume of the red blood cell fragments is overlapped with that of the lymphocytes, the white blood cells cannot be accurately counted, and the measured white blood cell count value is greatly different from an actual value (the actual value can be detected by an optical detection device); whereas if the dilution ratio is increased only by small amounts, e.g. to 550, 600, 650, 700, 800, etc., the measured white blood cell count value is still very different from the actual value.
The impedance method counting detection principle of the cells is as follows: a detection hole (namely a micropore) is arranged at the side part of the leucocyte counting cell, an electrode is respectively arranged on a channel at the two sides of the detection hole to form a positive electrode and a negative electrode, and the two electrodes are connected to a constant current source. When the sample liquid to be detected flows through the detection hole under the action of constant negative pressure, the resistance between the positive electrode and the negative electrode changes, the resistance forms pulse change proportional to the cell size of the blood sample, and a particle distribution curve reflecting the cell size of the blood sample can be drawn by collecting data of the pulse change, so that the detection data of the cells are obtained. The impedance method is adopted for counting and detecting the white blood cells, and compared with an optical detection method, the impedance method has the advantages of low cost and simple structure.
The first embodiment is as follows:
as shown in fig. 1 to 7, a cell analyzer for sheep, goats and cows according to an embodiment of the present invention includes an analyzer execution body 100, a human-computer interaction device 200 and a controller 300. The analyzer execution body 100 is configured to be capable of executing at least one test item on a blood sample. The human-computer interaction device 200 is configured to have an animal detection mode selection function, so that a user can select a corresponding detection mode according to actual conditions. The controller 300 is configured to control the analyzer execution main body 100 to execute corresponding test items on the blood sample according to the animal test mode selected by the human machine interface device 200. In a specific application, when the user selects a corresponding animal detection mode on the human-computer interaction device 200, the human-computer interaction device 200 feeds back a corresponding mode selection signal to the controller 300, and the controller 300 controls the analyzer execution main body 100 to detect the blood sample according to the corresponding detection mode according to the received mode selection signal.
As an embodiment, the analyzer execution body 100 is configured to be able to execute a white blood cell count detection item on a blood sample by using an impedance method, that is, the cell analyzer is able to perform a white blood cell impedance count detection on the blood sample.
The human-computer interaction device 200 is configured to be able to select an animal detection mode as follows: the detection device comprises a first type animal detection mode and a second type animal detection mode, wherein the first type animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode. In this embodiment, the cell analyzer can be used for performing impedance counting detection of white blood cells on a blood sample of at least one animal selected from the group consisting of sheep, goats, and cows.
The controller 300 is configured to: according to the animal detection mode selected by the human-computer interaction device 200, the analyzer execution main body 100 is controlled to execute the following actions on the blood sample:
when the animal detection mode selected by the human-computer interaction device 200 is the first type of animal detection mode, controlling the analyzer execution main body 100 to dilute the blood sample to a first preset proportion value through the diluent and the hemolytic agent to prepare a first detection sample solution, and controlling the analyzer execution main body 100 to perform white blood cell counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first counting value of the white blood cells;
when the animal detection mode selected by the human-computer interaction device 200 is the second animal detection mode, controlling the analyzer execution main body 100 to dilute the blood sample to a second preset proportion value through the diluent and the hemolytic agent to prepare a second detection sample solution, and controlling the analyzer execution main body 100 to perform white blood cell counting detection on the second detection sample solution by adopting an impedance method;
wherein, the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500:1, the second preset proportion value is greater than or equal to 100: 1 and less than or equal to 500: 1.
the hemolytic agent is mainly used for dissolving the red blood cells in the blood sample so as to avoid the interference of the red blood cells on the white blood cell counting, thereby being beneficial to improving the accuracy of the white blood cell counting; in addition, the hemolytic agent may also serve to dilute the blood sample. The diluent is mainly used for diluting blood sample cells to prevent blood sample cells from aggregating and adhering.
The cell analyzer provided by this embodiment prepares the blood sample of the sheep, goat or cow into the first detection sample liquid with a high dilution ratio (much higher than the dilution ratio of the cat, dog or horse) through the diluent and the hemolytic agent, and then performs the white blood cell count detection on the first detection sample liquid by using the impedance method, so that the more accurate white blood cell count value of the blood sample of the sheep, goat or cow can be obtained, thereby achieving the effect of accurately detecting the white blood cell count value of the sheep, goat or cow by using the impedance method. Because the cost of the first detection device 120 for impedance counting detection is far lower than that of an optical detection device for optical counting detection, the cost of the cell analyzer for detecting the white blood cells of sheep, goats and cows can be greatly reduced, and the cell analyzer is favorable for being widely popularized and applied in small and medium medical places and laboratories.
In one embodiment, the second animal detection mode includes at least one of a cat detection mode, a dog detection mode and a horse detection mode, i.e., the white blood cell detection mode of dogs, cats and horses can adopt the second animal detection mode.
In one embodiment, the second predetermined ratio is greater than or equal to 250 and less than or equal to 500, within which the white blood cell detection accuracy of the dog, cat or horse is high and the repeatability of the detection result is better (i.e. the difference between the results of multiple detections is smaller). If the dilution ratio is lower than 250, the linearity of the white blood cells is affected, and the accuracy of the test result of high-value white blood cells (white blood cells with high quantity) is affected; if the dilution ratio is greater than 500, the accuracy of the test result of low-value leukocytes (leukocytes with small number) is affected, and the repeatability of the test result is affected.
As a preferred embodiment of this embodiment, the cell analyzer is configured to detect white blood cells of dogs, cats, horses, sheep, goats, and cows, and the human-computer interaction device 200 is configured to select the following animal detection modes: a sheep detection mode, a goat detection mode, a cow detection mode, a cat detection mode, a dog detection mode, and a horse detection mode, and the controller 300 is configured to be able to control the analyzer execution main body 100 to perform white blood cell parameter detection on blood samples of sheep, goats, cows, dogs, cats, and horses by using an impedance method, respectively. When the blood sample is subjected to leucocyte counting detection in a sheep detection mode, a goat detection mode and a cow detection mode, diluting the blood sample to be more than or equal to 1200 by adopting diluent and hemolytic agent: 1 and less than or equal to 3500:1, in the above range. When the white blood cell count detection is carried out on the blood sample in the cat detection mode, the dog detection mode and the horse detection mode, the blood sample is diluted to be more than 250:1 and equal or equal to 500:1, in the above range. Of course, in a specific application, as an alternative embodiment, the animal detection mode that the human-computer interaction device 200 is configured to be able to select is not limited thereto, for example, two, three, four or five animal detection modes may be included, or the first type animal detection mode and the second type animal detection mode may also include only the detection modes of other animals.
As an embodiment, the first preset ratio value is greater than or equal to 1500:1 and less than or equal to 3000: 1. the blood sample of the sheep, the goat or the cow is diluted to be within the range, so that when the white blood cell count value of the blood sample of the sheep, the goat or the cow is detected by adopting an impedance method, the detection result is more accurate, and the error is within a controllable range.
As a preferred implementation of this embodiment, the first predetermined ratio is (2400 ± 200): 1, in the dilution range, the blood sample leukocyte count value of sheep, goats and cows is detected by adopting an impedance method, and the accuracy of the detection result value is very high.
As an embodiment, the analyzer execution main body 100 includes a sampling device 140, a diluent supply device, a hemolytic agent supply device, a white blood cell counting cell 110, and a first detection device 120, and the first detection device 120 is used for performing white blood cell count detection. The first detecting device 120 is a resistance detector, the first detecting device 120 includes a constant current source 121, an analyzing circuit and two electrodes 122, the white blood cell counting cell 110 is provided with a first chamber 112, a second chamber 113 and a detecting hole 111, the first chamber 112 is a main chamber of the white blood cell counting cell 110, the dilution treatment of the blood sample is performed in the first chamber 112, the second chamber 113 is connected with a negative pressure device 130, the detecting hole 111 communicates the first chamber 112 and the second chamber 113, and the detecting hole 111 is a micropore with a small pore size. Two electrodes 122 are respectively provided in the first chamber 112 and the second chamber 113, and a constant current source 121 is connected to the two electrodes 122. When the cells pass through the detection hole 111 under the action of the negative pressure device 130, corresponding pulses are generated, the larger the cell volume is, the larger the resistance is increased when the cells pass through the detection hole 111, so that the generated pulses are larger, namely, the amplitude of the pulses is in direct proportion to the cell volume, the frequency of the pulses is in direct proportion to the number of the cells, and the analysis circuit can draw a particle distribution curve reflecting the cell size of the blood sample by collecting data of changes of the pulses, so that the detection data of the cells are obtained. The white blood cell counting cell 110 is used for preparing a blood sample into a first detection sample solution, and white blood cell counting detection is directly performed on the white blood cell counting cell 110, that is, the white blood cell counting cell 110 is used for providing a reaction (dilution, hemolysis, blending, etc.) place for the blood sample and also providing a detection place for white blood cell counting detection of the blood sample, so that the reduction of the number of components, the cost and the volume of the cell analyzer is facilitated.
The sampling device 140 includes a sampling needle, a sample suction line, a liquid suction and discharge driving device, and a motion driving device. The sample suction pipeline is respectively communicated with the sampling needle and the liquid suction and discharge driving device. The aspiration and drainage fluid driving device can provide a driving force for sucking fluid for the sampling needle and can provide a driving force for discharging fluid for the sampling needle. The sampling needle is installed on the motion drive arrangement, and the motion drive arrangement can drive the sampling needle and carry out spatial movement to make the sampling needle move to different stations, for example stand-by position, sampling position, branch appearance position etc..
As an embodiment, the analyzer execution body 100 is further configured to be able to execute a leukocyte classification test item on the blood sample, that is, in the present embodiment, the cell analyzer can accurately detect leukocyte classification data of sheep or goats or cows in addition to accurately detecting a leukocyte count value of the sheep or goats or cows. Specifically, the controller 300 is configured to: when the animal detection mode selected by the human-computer interaction device 200 is the first type animal detection mode, the analyzer execution main body 100 is controlled to execute the following actions: diluting the blood sample to a third preset proportion value through a diluent and a hemolytic agent to prepare a third detection sample solution; carrying out white blood cell counting detection on at least part of the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells; diluting at least part of the third detection sample solution to a first preset proportion value by using a hemolytic agent and a diluent to prepare a first detection sample solution; carrying out white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data; wherein the third preset proportion value is greater than or equal to 1200:1 and less than a first preset proportional value. In this embodiment, the third detection sample solution is mainly used for classifying and detecting leukocytes, and the hemolytic agent added in the preparation process can dissolve erythrocytes, and simultaneously can separate leukocytes into three groups, namely, a lymphocyte group, a monocyte group and a granulocyte group, under the action of the hemolytic agent, so as to facilitate classification of leukocytes. The first detection sample liquid is mainly used for counting detection of white blood cells. Wherein, the hemolytic agent added for the first time during the preparation of the third detection sample solution is preferably not to affect the classification of the white blood cells; the hemolytic agent added in the second time when the first test sample solution is prepared is preferably such that red blood cell debris is not visible in the histogram. This embodiment, through diluting twice, hemolysis and detecting to the blood sample, can do benefit to the classification and the count detection that realize leucocyte, improved the accuracy that the categorised count of leucocyte detected. Of course, in specific applications, as an alternative embodiment, the cell analyzer suitable for sheep, goat and cow does not necessarily need to prepare the third detection sample solution and perform the white blood cell count detection on the third detection sample solution, but only performs the white blood cell count detection on the first detection sample solution prepared by diluting the blood sample.
In this embodiment, the first test sample solution is obtained by further lysing red blood cells and diluting the third test sample solution, which is favorable for reducing the consumption of the blood sample, the diluent and the hemolytic agent. In addition, the first detection sample solution and the third detection sample solution are prepared in the same leukocyte counting cell 110, and a preparation place for the third detection sample solution is not required to be separately provided, so that the low-cost and small-volume design of the cell analyzer is facilitated. Of course, in a specific application, as an alternative embodiment, the first test sample solution and the third test sample solution may be prepared independently, that is, the first test sample solution is prepared by lysing erythrocytes and diluting one blood sample to prepare the first test sample solution, and the third test sample solution is prepared by lysing erythrocytes and diluting another blood sample to prepare the third test sample solution.
As an embodiment, the controller 300 is further configured to: and analyzing and processing the first detection data and the second detection data to obtain the classification counting data of the white blood cells. In this embodiment, the first detection data may obtain an accurate white blood cell count value, and the first detection data and the second detection data may be analyzed to obtain an accurate white blood cell classification count value.
Referring to fig. 4 to 6, the first detection data includes a first histogram (i.e., fig. 5) which is a histogram of the number and volume distribution of white blood cells and a first count value of white blood cells; the second detection data comprises a second histogram (namely figure 4) and a second counting value of the white blood cells, and the second histogram is a histogram of the number and volume distribution of the red blood cell fragments and the white blood cells; the embodiment of the controller 300 analyzing and processing the first detection data and the second detection data to obtain the differential count data of the white blood cells includes: calculating S1 and S2 (i.e., S1 and S2 in fig. 4) according to formula S1/(S1+ S2) ═ WBC1/WBC2, and determining a boundary between the red cell debris and the white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram by a boundary to obtain a third histogram (namely, figure 6); classifying and counting the white blood cells in the third histogram according to the volume size to obtain classified counting data of the white blood cells; where S1 is the distribution area of white blood cells in the first histogram, S2 is the distribution area of red blood cell debris in the second histogram, WBC1 is the first count value, and WBC2 is the second count value. The first histogram obtains an accurate white blood cell count value, but it cannot obtain a classification value of white blood cells; in the second histogram, the cells are classified into a lymphocyte population, a monocyte population and a granulocyte population according to the size of the cells, but the lymphocyte population contains red blood cell debris, and therefore the counted number is higher than the actual number of white blood cells. According to the embodiment, red blood cell fragments in the second histogram are removed according to a formula S1/(S1+ S2) ═ WBC1/WBC2 to obtain a third histogram, and the lymphocyte, the monocyte and the granulocyte can be classified according to the size of the cell, so that an accurate white blood cell classification count value can be obtained.
In this example, the classification of the white blood cells can be obtained by diluting the blood sample with the hemolytic agent and the diluent for the first time, but since it contains red blood cell debris, its counting is not very accurate; the blood sample is further diluted by adding the hemolytic agent and the diluent for the second time, so that the red blood cell fragments can be further reduced, the red blood cell fragments can not be seen in the histogram, and the accurate white blood cell classification counting value can be obtained.
As an embodiment, the third preset proportion value is greater than or equal to 1200:1 and less than or equal to 2000: within the range, the method is favorable for obtaining more accurate leukocyte classification detection data.
As a preferred implementation of this embodiment, the third predetermined ratio is (1800 ± 200): 1.
as an embodiment, the analyzer execution body 100 is further configured to be able to execute a red blood cell count test item on the blood sample, the analyzer execution body 100 further comprises a red blood cell count cell and a second test device for performing a red blood cell count test;
the controller 300 is configured to: when the animal detection mode selected by the human-computer interaction device 200 is the first type animal detection mode, the analyzer execution main body 100 is controlled to execute the following actions:
controlling the sampling device 140 to collect a blood sample and adding the collected blood sample to the white blood cell counting cell 110;
controlling the diluent supply device to add diluent into the white blood cell counting cell 110 so as to dilute the blood sample to a fourth preset proportion value, and preparing a fourth detection sample solution;
controlling the sampling device 140 to collect a first portion of the fourth detection sample solution from the white blood cell counting cell 110, and controlling the sampling device 140 to add the collected first portion of the fourth detection sample solution into the red blood cell counting cell for dilution and red blood cell counting detection;
controlling the sampling device 140 to collect a second portion of the fourth testing sample solution from the white blood cell counting cell 110;
as an embodiment, the analyzer execution main body 100 may further include a drainage pipeline, a drainage valve, and a waste liquid pool, the drainage pipeline is connected between the white blood cell counting cell 110 and the waste liquid pool, the drainage valve is disposed on the drainage pipeline for controlling on/off of the drainage pipeline, and the blank cell counting cell 110 may be drained by controlling the opening of the drainage valve;
controlling the sampling device 140 to add at least a part of the fourth detection sample solution in the second collected part of the fourth detection sample solution into the white blood cell counting cell 110;
controlling the diluent supply device to deliver diluent to the white blood cell counting cell 110, and controlling the hemolytic agent supply device to deliver hemolytic agent to the white blood cell counting cell 110 to dissolve red blood cells in the fourth detection sample solution, and diluting the blood sample to a first preset proportion value to prepare a third detection sample solution;
controlling the first detection device 120 to perform white blood cell counting detection on the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells;
controlling the diluent supply device to deliver diluent to the white blood cell counting cell 110, and controlling the hemolytic agent supply device to deliver hemolytic agent to the white blood cell counting cell 110, so as to dilute the third detection sample solution to a first preset ratio value, and preparing a first detection sample solution;
controlling the first detection device 120 to perform white blood cell counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data;
and the fourth preset proportion value is smaller than the third preset proportion value.
In this embodiment, the cell analyzer can accurately detect the white blood cell classification and count value of the sheep, goat or cow, and can also accurately detect the red blood cell count value of the sheep, goat or cow. In addition, in the present embodiment, the blood sample is diluted with the diluent to form the fourth detection sample solution in the white blood cell counting chamber 110, then the first portion of the fourth detection sample solution is collected by the sampling device 140 and distributed to the red blood cell counting chamber, the second portion of the fourth detection sample solution is collected, then the remaining fourth detection sample solution in the white blood cell counting chamber 110 is emptied, at least a portion of the fourth detection sample solution in the sampling device 140 is added to the white blood cell counting chamber 110 for dilution and hemolysis to prepare the detection sample solution for white blood cell detection, because of the intermediate emptying operation, only a portion of the fourth detection sample solution is diluted to a high dilution ratio, instead of diluting all the fourth detection sample solution except the sample solution for red blood cell detection, so that on one hand, the amount of the diluent solution for diluting the blood sample to the high dilution ratio is reduced, on the other hand, the volume of the white blood cell count cell 110 does not need to be increased on the premise that a high dilution ratio can be realized.
In this embodiment, a part of the fourth detection sample solution is used for performing red blood cell count detection, and a part of the fourth detection sample solution is used for preparing the third detection sample solution, that is, the third detection sample solution is obtained by further processing the fourth detection sample solution, which is beneficial to reducing consumption of the blood sample and the diluent. In this embodiment, the first detection sample solution, the third detection sample solution, and the fourth detection sample solution are prepared in the same white blood cell counting cell 110, and there is no need to separately provide preparation places for the third detection sample solution and the fourth detection sample solution, which is beneficial to realizing low cost and small volume design of the cell analyzer. Of course, in specific applications, as an alternative embodiment, the preparation of the third testing sample solution and the preparation of the fourth testing sample solution can be performed independently, that is, the third testing sample solution is prepared by performing hemolysis and dilution on one blood sample to prepare the third testing sample solution, and the fourth testing sample solution is prepared by performing hemolysis and dilution on another blood sample to prepare the fourth testing sample solution; as another alternative embodiment, the first test sample liquid may be obtained by directly processing the fourth test sample liquid.
As an embodiment, the strand controller is configured to: the sampling device 140 is controlled to add a part (i.e. a third part) of the collected second part of the fourth detection sample solution into the white blood cell counting cell 110 for preparing the third detection sample solution and the first detection sample solution, instead of adding the whole collected second part of the fourth detection sample solution into the white blood cell counting cell 110, so as to facilitate accurate control of the volume of the fourth detection sample solution added into the white blood cell counting cell 110. Of course, in a specific application, as an alternative embodiment, the sampling device 140 may be controlled to completely add the collected second portion of the fourth detection sample solution into the white blood cell counting cell 110 for preparing the third detection sample solution and the first detection sample solution.
As an embodiment, the controller 300 is further configured to: before controlling the sampling device 140 to collect a blood sample and adding the collected blood sample to the white blood cell counting chamber 110, the diluent supply device is controlled to add a diluent to the white blood cell counting chamber 110 to form a base solution.
In one embodiment, in the preparation process of the first detection sample solution, after the hemolytic agent, the diluent, and the blood sample are proportionally added to the leukocyte counting cell 110, the mixing operation of the hemolytic agent, the diluent, and the blood sample is further included. In a preferred embodiment, the bottom of the white blood cell counting cell 110 is provided with a vent connected with the air path system, and the blending operation is to blow air from the bottom of the white blood cell counting cell 110; of course, in alternative embodiments, blending may be performed in other ways, such as, for example, with a stir bar, for example, for specific applications.
In one embodiment, the preparation process of the third detection sample solution and the preparation process of the fourth detection sample solution also include a blending operation, and the blending manner can refer to the blending manner in the preparation of the first detection sample solution, and will not be described in detail herein.
The embodiment also provides a cell detection method suitable for sheep, goats and cows, which comprises a first detection sample liquid preparation step and a first detection step. Wherein the first detection sample liquid preparation step comprises: adopting hemolytic agent and diluent to dilute the blood sample to a first preset proportion value, and preparing a first detection sample solution, wherein the first preset proportion value is larger than or equal to 1200:1 and less than or equal to 3500: 1. the first detecting step includes: and carrying out white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain counting detection data of white blood cells. The hemolytic agent is mainly used for dissolving the red blood cells in the blood sample so as to avoid the interference of the red blood cells on the white blood cell counting, thereby being beneficial to improving the accuracy of the white blood cell counting; in addition, the hemolytic agent may also serve to dilute the blood sample. The diluent is mainly used for diluting blood sample cells to prevent blood sample cells from aggregating and adhering. In the embodiment, the impedance method is adopted to detect the detection sample liquid with high dilution ratio, so that the accuracy of the blood sample leukocyte count value of sheep, goats and cows can be effectively improved on the premise of low cost.
Referring to fig. 1 to 3, as an embodiment, the first detecting step includes: the first detection device 120 is controlled to perform white blood cell count detection on the first detection sample solution to obtain first detection data of white blood cells, wherein the first detection data includes a first count value of the white blood cells. The principle of the first detecting unit 120 detecting the white blood cell count value by the impedance method can be referred to the description of the cell analyzer, and will not be described in detail herein.
The preferred setting range of the first preset ratio value can be referred to the description of the cell analyzer, and will not be described in detail.
In one embodiment, the cell detection method for sheep, goat and cow further comprises a sampling step and a first sampling step. The sampling step is used to collect a blood sample. The first sampling step is used to transfer at least a portion of the blood sample collected in the sampling step to the white blood cell counting chamber 110, i.e., to distribute the blood sample to the white blood cell counting chamber 110. The first testing sample solution preparing step is used for diluting at least a portion of the blood sample distributed to the leukocounting cell 110 in the first sample distributing step to a first predetermined ratio value to prepare a first testing sample solution.
In one embodiment, the first detection step is used to perform a white blood cell count detection on the first detection sample solution in the white blood cell count cell 110. In this embodiment, the white blood cell counting cell 110 is used for preparing a blood sample into a first detection sample solution, and white blood cell counting detection is directly performed in the white blood cell counting cell 110, that is, the white blood cell counting cell 110 is used for providing a processing place for dilution and hemolysis of the blood sample and also providing a detection place for white blood cell counting detection of the blood sample, so that the reduction of the number of components, the cost and the volume of the cell analyzer is facilitated.
In one embodiment, the cell detection method for sheep, goat and cow further comprises a third detection sample solution preparation step and a second detection step. The third detection sample liquid preparation step comprises: adopting hemolytic agent and diluent to dilute at least part of blood sample to a third preset proportion value to prepare a third detection sample solution, wherein the third preset proportion value is larger than or equal to 1200:1 and less than a first preset proportional value. The second detecting step includes: and controlling the first detection device 120 to perform leucocyte counting detection on at least part of the third detection sample solution by using an impedance method to obtain second detection data. The third detection sample liquid is mainly used for classifying and detecting the white blood cells, and the hemolytic agent added in the preparation process can dissolve the red blood cells and simultaneously can enable the white blood cells to be divided into three groups of lymphocyte group, monocyte group and granulocyte group under the action of the hemolytic agent, so that the classification of the white blood cells is facilitated. The first detection sample liquid is mainly used for counting detection of white blood cells. This embodiment, through diluting twice and detecting the blood sample, can do benefit to the classification and the count detection that realize the leucocyte, improved the accuracy of leucocyte classification and count detection. Of course, in specific applications, the cell detection method suitable for sheep, goats and cows does not need to be provided with a third detection sample liquid preparation step and a second detection step as an alternative embodiment, and only blood samples are counted and detected.
As one embodiment, the first detection sample liquid preparation step is performed by: and (3) diluting at least part of the third detection sample solution to a first preset proportion value by using a diluent and a hemolytic agent to prepare the first detection sample solution, namely in the embodiment, the first detection sample solution is obtained by further dissolving red blood cells and diluting the third detection sample solution, so that the consumption of the blood sample, the diluent and the hemolytic agent is favorably reduced. Of course, in a specific application, as an alternative embodiment, the first detection sample solution preparation step and the third detection sample solution preparation step may be performed independently, that is, the first detection sample solution preparation step performs red blood cell lysis and dilution on one blood sample to prepare a first detection sample solution, and the third detection sample solution preparation step performs red blood cell lysis and dilution on the other blood sample to prepare a third detection sample solution.
The preferred setting range of the third preset ratio can be referred to the description of the cell analyzer, and will not be described in detail.
As one embodiment, the cell detection method further comprises a data analysis step, the data analysis step comprising: and analyzing and processing the first detection data and the second detection data to obtain the classification counting data of the white blood cells. In this embodiment, the first detection data may obtain an accurate white blood cell count value, and the first detection data and the second detection data may be analyzed to obtain an accurate white blood cell classification count value.
Referring to fig. 4 to 6, as an embodiment, the first detection data includes a first histogram (i.e., fig. 5) which is a histogram of the number and volume distribution of white blood cells and a first count value of white blood cells; the second detection data comprises a second histogram (namely figure 4) and a second counting value of the white blood cells, and the second histogram is a histogram of the number and volume distribution of the red blood cell fragments and the white blood cells; the data analysis step comprises: calculating S1 and S2 (i.e., S1 and S2 in fig. 4) according to formula S1/(S1+ S2) ═ WBC1/WBC2, and determining a boundary between the red cell debris and the white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram by a boundary to obtain a third histogram (namely, figure 6); classifying and counting the white blood cells in the third histogram according to the volume size to obtain classified counting data of the white blood cells; where S1 is the distribution area of white blood cells in the first histogram, S2 is the distribution area of red blood cell debris in the second histogram, WBC1 is the first count value, and WBC2 is the second count value. The first histogram obtains an accurate white blood cell count value, but it cannot obtain a classification value of white blood cells; in the second histogram, the cells are classified into a lymphocyte population, a monocyte population and a granulocyte population according to the size of the cells, but the lymphocyte population contains red blood cell debris, and therefore the counted number is higher than the actual number of white blood cells. According to the embodiment, red blood cell fragments in the second histogram are removed according to a formula S1/(S1+ S2) ═ WBC1/WBC2 to obtain a third histogram, and the lymphocyte, the monocyte and the granulocyte can be classified according to the size of the cell, so that an accurate white blood cell classification count value can be obtained.
In one embodiment, the cell detection method further includes a fourth detection sample solution preparation step and a third detection step, and the fourth detection sample solution preparation step includes: and (4) diluting the blood sample to a fourth preset proportion value by using the diluent, and preparing a fourth detection sample solution. The third detection step comprises: and diluting at least a part of the fourth detection sample solution to prepare a fifth detection sample solution, and controlling the second detection device to perform red blood cell counting detection on the fifth detection sample solution. In this embodiment, the cell detection method can realize both the differential counting detection of white blood cells and the counting detection of red blood cells.
In one embodiment, the fourth detection sample liquid preparation step is performed after the first sampling step and before the third detection sample liquid preparation step. The cell detection method further includes a second sample separation step after the fourth detection sample liquid preparation step and before the third detection step, the second sample separation step including: and delivering the third part of the fourth detection sample liquid to the erythrocyte counting cell. The third detection sample liquid preparation step is implemented as follows: and dissolving the first part of the fourth detection sample solution by using a diluent and a hemolytic agent, and diluting the first part of the fourth detection sample solution to a third preset proportion value to prepare a third detection sample solution, wherein the fourth preset proportion value is smaller than the third preset proportion value. In this embodiment, a part of the fourth detection sample solution is used for performing red blood cell count detection, and a part of the fourth detection sample solution is used for preparing the third detection sample solution, that is, the third detection sample solution is obtained by further processing the fourth detection sample solution, so that the consumption of the blood sample and the diluent is favorably reduced. Of course, in specific applications, as an alternative embodiment, the third detection sample solution preparation step and the fourth detection sample solution preparation step may be performed independently, that is, the third detection sample solution preparation step hemolyzes and dilutes one blood sample to prepare a third detection sample solution, and the fourth detection sample solution preparation step hemolyzes and dilutes another blood sample to prepare a fourth detection sample solution; as another alternative embodiment, the first test sample liquid may be obtained by directly processing the fourth test sample liquid.
In one embodiment, the second sample dividing step further includes, after the third portion of the fourth test sample solution is transferred to the red blood cell count cell: sucking a first portion of the fourth detection sample solution from the white blood cell count cell 110 by the sampling device 140; a blank cell counting cell 110 is arranged, and a diluent is added into the white cell counting cell 110 to form a bottom solution; the first portion of the fourth testing sample fluid in the sampling device 140 is delivered to the leukocyte testing cell. The method of emptying first and then diluting part of the sample liquid again instead of diluting all the detection sample liquid is favorable for reducing the consumption of the diluent and reducing the volume of the leucocyte counting cell 110.
Referring to fig. 1 to 3, as an embodiment, the sampling step includes: the control sampling device 140 collects a blood sample in a blood sample container. The specific structure of the sampling device 140 can be referred to the description of the cell analyzer, and will not be described in detail. When the sampling step is implemented, the movement driving device drives the sampling needle to move to the upper part of the blood sample container, then the sampling needle is driven to extend into the blood sample container and be inserted into the blood sample in the blood sample container, the liquid suction and discharge driving device drives the sampling needle to suck the blood sample, then the movement driving device drives the sampling needle to move away from the blood sample container, and thus the sampling step is completed.
As an embodiment, the first sampling step includes: the sampling device 140 is controlled to deliver at least a portion of the collected blood sample to the white blood cell count cell 110. When the first sample dividing step is carried out, the movement driving device drives the sampling needle to move to the position above the leucocyte counting cell 110, then the sampling needle is driven to extend into the leucocyte counting cell 110, the liquid suction and discharge driving device drives the sampling needle to discharge a blood sample, and then the movement driving device drives the sampling needle to move away from the leucocyte counting cell 110, so that the first sample dividing step is completed.
As an embodiment, between the sampling step and the first sampling step, the method further comprises the steps of: a dilution solution is added to the white blood cell count cell 110 to form a bottom solution.
As an embodiment, the fourth detection sample liquid preparation step is implemented by: and controlling the diluent supply device to deliver diluent to the white blood cell counting cell 110 so as to dilute the blood sample to a fourth preset proportion value, thereby preparing a fourth detection sample solution. In this embodiment, the fourth detection sample solution is prepared in the leukocyte counting cell 110, and a preparation place for the fourth detection sample solution is not required to be separately provided, which is beneficial to realizing the low-cost and small-volume design of the cell analyzer.
In one embodiment, the third detection sample solution preparation step includes: controlling the diluent supply device to deliver diluent to the white blood cell counting cell 110, and controlling the hemolytic agent supply device to deliver hemolytic agent to the white blood cell counting cell 110 to dissolve red blood cells in the blood sample, and diluting the blood sample to a third predetermined ratio value to prepare a third detection sample solution.
As one embodiment, the third detection sample liquid preparation step is performed after the fourth detection sample liquid preparation step and before the first detection sample liquid preparation step, and includes: and (3) dissolving the red blood cells of the third part of the fourth detection sample solution by adopting a diluent and a hemolytic agent, and diluting the third part of the fourth detection sample solution to a third preset proportion value to prepare a third detection sample solution. In this embodiment, the third detection sample solution is prepared in the white blood cell counting cell 110, and a preparation place for the third detection sample solution is not required to be separately provided, which is beneficial to realizing the low-cost and small-volume design of the cell analyzer.
As a preferred implementation of this embodiment, the second sampling step includes: controlling the sampling device 140 to collect a first portion of the third detection sample from the white blood cell counting cell 110, and controlling the sampling device 140 to add the collected first portion of the fourth detection sample liquid into the red blood cell counting cell; the third detection sample liquid preparation step comprises: controlling the sampling device 140 to collect a second portion of the third detection sample from the white blood cell counting cell 110, and controlling the discharge of the remaining fourth detection sample solution in the blank white blood cell counting cell 110; controlling the diluent supply device to deliver diluent to the white blood cell counting cell 110 to form a bottom solution, controlling the sampling device 140 to add a third portion of the fourth detection sample solution to the white blood cell counting cell 110 (the third portion of the fourth detection sample solution belongs to at least one part of the second portion of the fourth detection sample solution collected by the sampling device 140), controlling the diluent supply device to deliver the diluent to the white blood cell counting cell 110, and controlling the hemolytic agent supply device to deliver hemolytic agent to the white blood cell counting cell 110 to dissolve red blood cells in the blood sample, and diluting the blood sample to a third preset ratio value to prepare a third detection sample solution. In this embodiment, the third test sample volume of the second portion is greater than the third test sample volume of the third portion; of course, in certain applications, the third test sample volume of the second portion may be set equal to the third test sample volume of the third portion, as an alternative embodiment.
As an embodiment, the second detecting step includes: and controlling the first detection device 120 to perform white blood cell counting detection on the third detection sample solution to obtain second detection data. In a preferred embodiment of this embodiment, the third detection sample liquid preparation step, the second detection step, and the first detection sample liquid preparation step are sequentially performed in the same white blood cell counting cell 110, that is, the second detection step is performed after the third detection sample liquid preparation step and before the first detection sample liquid preparation step, which is beneficial to preventing the hemolytic agent added in the first detection sample liquid preparation step from affecting the second detection data detected in the second detection step, thereby achieving the effect of improving the accuracy of white blood cell classification and counting of sheep, goats, and cows on the premise of low cost.
As one embodiment, the first detection sample liquid preparation step includes: controlling the diluent supply device to deliver diluent to the white blood cell counting cell 110, and controlling the hemolytic agent supply device to deliver hemolytic agent to the white blood cell counting cell 110 to dissolve red blood cells in the blood sample, and diluting the blood sample to a first preset ratio value to prepare a first detection sample solution. In a preferred embodiment of this embodiment, the first step of preparing the test sample solution is performed by: and (3) releasing the third detection sample solution after the second detection step to a third preset proportion value by adopting a diluent and a hemolytic agent to prepare the first detection sample solution.
As an embodiment, an embodiment of the third detecting step includes: and controlling the diluent supply device to convey diluent to the red blood cell counting pool so as to dilute a third part of fourth detection sample liquid in the red blood cell counting pool to prepare a fifth detection sample liquid, and controlling the second detection device to perform red blood cell counting detection on the fifth detection sample liquid. The third detection step may be performed starting at any time period after the second sampling step is completed.
In one embodiment, the first step of preparing the sample solution for detection further includes mixing the hemolyzing agent, the diluent, and the blood sample after proportionally adding the hemolyzing agent, the diluent, and the blood sample into the white blood cell counting cell 110. The specific mixing method can be referred to the above description of the cell analyzer, and will not be described in detail here.
In one embodiment, the third detection sample liquid preparation step and the fourth detection sample liquid preparation step further include a mixing operation, and the mixing manner may refer to the mixing manner in the first detection sample liquid preparation step, and will not be described in detail herein.
In a preferred embodiment of this embodiment, the method for detecting a blood sample in the second type animal detection mode (i.e., a cell detection method for a horse, a dog or a cat) comprises the following steps:
adding 1350uL of the diluent into the leucocyte counting cell 110 to form a base solution;
adding a 9uL blood sample into the leucocyte counting cell 110, simultaneously adding 550uL diluent, and flushing a sampling needle;
blowing air into the leukocyte counting cell 110 from the bottom of the leukocyte counting cell 110, and uniformly mixing the liquid in the leukocyte counting cell 110 to obtain a fourth detection sample liquid;
sucking out the uniformly mixed 20uL fourth detection sample liquid, and adding the fourth detection sample liquid into a red blood cell counting pool for counting and detecting red blood cells;
at this time, the volume of the diluent in the leukocyte counting cell 110 is (1350+550) × (1350+550+9-20)/(1350+550+9) ≈ 1880.0943uL, and the volume of the blood sample is 9 × (1350+550+9-20)/(1350+550+9) ≈ 8.9057 uL;
300uL of hemolytic agent and 400uL of diluent are added to the white blood cell counting cell 110, and the dilution ratio in the white blood cell counting cell 110 is about (1880.0943+300+400)/8.9057 ≈ 289: 1.
Blowing air into the leukocyte counting cell 110 from the bottom of the leukocyte counting cell 110, and uniformly mixing the liquid in the leukocyte counting cell 110 to prepare a second detection sample liquid;
and detecting the second detection sample liquid by adopting an impedance method to obtain the white blood cell detection data of the blood sample of the horse, the dog or the cat.
If the white blood cell detection of the blood sample of the sheep is carried out by adopting the detection method of the blood sample in the second animal detection mode (namely the cell detection method of a horse, a dog or a cat), namely the white blood cell parameter is detected by adopting the dilution ratio of 250-500, a histogram shown in fig. 7 is obtained, and as can be seen from the histogram, the volume of red blood cell fragments is overlapped with that of lymphocytes, the red blood cell fragments cannot be filtered from the histogram, so that the accurate test result of the white blood cells of the blood sample of the sheep cannot be obtained.
As a preferred embodiment of this embodiment, the method for detecting a blood sample in the first type animal detection mode (i.e., the method for detecting cells in sheep or goat or cow) comprises the following steps:
adding 1350uL of the diluent into the leucocyte counting cell 110 to form a base solution;
adding a 9uL blood sample into the leucocyte counting cell 110, simultaneously adding 550uL diluent, and flushing a sampling needle;
blowing air into the leukocyte counting cell 110 from the bottom of the leukocyte counting cell 110, and uniformly mixing the liquid in the leukocyte counting cell 110 to obtain a fourth detection sample liquid;
sucking out the uniformly mixed 20uL fourth detection sample liquid, and adding the fourth detection sample liquid into a red blood cell counting pool for counting and detecting red blood cells;
the sampling needle is placed into the leucocyte counting cell 110 again, and 320uL of fourth detection sample liquid is pumped into the sampling needle and a sample suction pipeline connected with the sampling needle;
emptying the white blood cell counting cell 110;
1200mL of the dilution was added to the white blood cell count cell 110 to form a bottom solution. Adding 305uL of liquid in the sampling needle and the sample suction pipeline into the leucocyte counting cell 110, simultaneously adding 550uL of diluent, and flushing the sampling needle and the sample suction pipeline;
then 300uL of hemolytic agent and 400uL of diluent are added to the white blood cell counting cell 110, and the dilution ratio in the white blood cell counting cell 110 is about 1800: 1. The hemolytic agent is used for dissolving red blood cells, and white blood cells are divided into several groups under the action of the hemolytic agent, such as lymphocyte groups, monocyte groups, granulocyte groups and the like;
blowing air into the leukocyte counting cell 110 from the bottom of the leukocyte counting cell 110, and uniformly mixing the liquid in the leukocyte counting cell 110 to prepare a third detection sample liquid;
detecting the third detection sample liquid to obtain second detection data; during detection, under the action of negative pressure, liquid in the leucocyte counting cell 110 passes through the detection hole 111 of the leucocyte counting cell 110, and a signal of the leucocyte counting cell 110 is tested, so that second detection data can be obtained; the count value of white blood cells in the second test data, which includes the results of some red blood cell debris, is higher than the actual value, and is WBC 2;
300uL of hemolytic agent and 400uL of diluent were added to the white blood cell counting cell 110, and the dilution ratio of the white blood cell counting cell 110 was about 2400: 1.
Blowing air into the leukocyte counting cell 110 from the bottom of the leukocyte counting cell 110, and uniformly mixing the liquid in the leukocyte counting cell 110 to obtain a first detection sample liquid.
The first detection sample solution is detected to obtain first detection data, and the first detection sample solution is prepared by adding the hemolytic agent and the diluent again on the basis of the third detection sample solution, so that the red blood cell fragments in the third detection sample solution are further reduced under the action of the hemolytic agent, and the red blood cell fragments cannot be seen on the white blood cell histogram. The count value of the white blood cells in the first detection data is consistent with the actual white blood cell value and is WBC 1;
removing red blood cell fragments on the left side of the white blood cell first counting histogram (namely, a second histogram obtained by detecting a third detection sample solution, namely, a graph shown in fig. 4) through WBC1 and WBC2, calculating S1 and S2 according to a formula S1/(S1+ S2) ═ WBC1/WBC2, and determining boundary lines between the red blood cell fragments and the white blood cells in the second histogram according to the calculated S1 and S2; removing the distribution area of the red blood cell fragments in the second histogram by a boundary to obtain a third histogram (namely, figure 6); and classifying and counting the white blood cells in the third histogram according to the volume size to obtain the classification and counting data of the white blood cells, such as the classification and counting of lymphocytes, monocytes and granulocytes. Where S1 is a distribution area of white blood cells in the first histogram, S2 is a distribution area of red blood cell debris in the second histogram, WBC1 is a first count value, and WBC2 is a second count value.
The applicant carried out leucocyte impedance counting tests on the blood samples of horses with different dilution ratios, obtaining the following results:
the white blood cell count (WBC) was 8.22 x 10 at a dilution ratio of 289:19/L；
When the dilution ratio is 400:1, the number of detected white blood cells is 8.32 x 109/L；
When the dilution ratio is 800:1, the number of detected white blood cells is 8.01 x 109/L；
When the dilution ratio is 1000:1, the number of the white blood cells is detectedThe value was 7.92 x 109/L；
When the dilution ratio is 1800:1, the number of detected white blood cells is 8.02 x 109/L；
When the dilution ratio is 2400:1, the number of detected white blood cells is 8.45 x 109/L。
Wherein the actual white blood cell count (measured by a cell analyzer with an optical detection device) of the horse blood sample is 8.22 × 109And L. It follows that, not the greater the dilution ratio, the more accurate the impedance count of the horse's blood sample leukocytes.
The applicant carried out leucocyte impedance counting tests on blood samples taken from sheep using different dilution ratios, obtaining the following results:
when the dilution ratio is 289:1, the number of detected white blood cells is 95.62 × 109/L；
When the dilution ratio is 500:1, the number of detected white blood cells is 85.12 x 109/L；
When the dilution ratio is 1200:1, the number of detected white blood cells is 26.85 x 109/L；
When the dilution ratio is 1500:1, the number of detected white blood cells is 20.65 x 109/L；
When the dilution ratio is 1800:1, the number of detected white blood cells is 19.36 x 109/L；
When the dilution ratio is 2400:1, the number of detected white blood cells is 12.51 x 109/L；
When the dilution ratio is 3000:1, the number of detected white blood cells is 13.02 x 109/L；
When the dilution ratio is 3500:1, the number of detected white blood cells is 12.32 x 109/L。
Wherein the actual white blood cell count value (measured by a cell analyzer having an optical detection device) of the sheep blood sample is 12.51 × 109And L. Therefore, when the white blood cell count value of the blood sample of the sheep is detected by adopting the impedance method, the detection result of the dilution ratio between 1200:1 and 3500:1 is more accurate than the detection result of the dilution ratio between 250:1 and 500:1, and when the dilution ratio is in a certain high dilution ratio range, the meter is usedThe value is relatively close to the actual count value.
The applicant carried out leucocyte impedance counting tests on goat blood samples using different dilution ratios, obtaining the following results:
when the dilution ratio is 289:1, the number of detected white blood cells is 65.32 x 109/L；
When the dilution ratio is 500:1, the number of detected white blood cells is 58.36 × 109/L；
When the dilution ratio is 1200:1, the number of detected white blood cells is 43.56 x 109/L；
When the dilution ratio is 1500:1, the number of detected white blood cells is 29.68 x 109/L；
When the dilution ratio is 1800:1, the number of detected white blood cells is 23.78 x 109/L；
When the dilution ratio is 2400:1, the number of detected white blood cells is 12.34 x 109/L；
When the dilution ratio is 3000:1, the number of detected white blood cells is 11.68 x 109/L；
When the dilution ratio is 3500:1, the number of detected white blood cells is 13.02 x 109/L。
Wherein the actual count value of leukocytes in goat blood sample (measured by cell analyzer with optical detection device) is 12.65 × 109And L. Therefore, when the impedance method is adopted to detect the white blood cell count value of a goat blood sample, the detection result with the dilution ratio of 1200: 1-3500: 1 is higher than the detection result with the dilution ratio of 250: 1-500: 1, and when the dilution ratio is in a certain high dilution ratio range, the count value is closer to the actual count value.
The applicant carried out leucocyte impedance counting tests on the blood samples of cows with different dilution ratios, obtaining the following results:
when the dilution ratio is 289:1, the number of detected white blood cells is 32.52 x 109/L；
When the dilution ratio is 500:1, the number of detected white blood cells is 23.56 x 109/L；
White was detected at a dilution ratio of 1200:1The number of cells was 20.54 x 109/L；
When the dilution ratio is 1500:1, the number of detected white blood cells is 18.98 x 109/L；
When the dilution ratio is 1800:1, the number of detected white blood cells is 13.23 x 109/L；
When the dilution ratio is 2400:1, the number of detected white blood cells is 13.65 x 109/L；
When the dilution ratio is 3000:1, the number of detected white blood cells is 12.35 x 109/L；
When the dilution ratio is 3500:1, the number of detected white blood cells is 12.39 x 109/L。
Wherein the actual white blood cell count value (measured by cell analyzer with optical detection device) of cow blood sample is 12.35 × 109And L. Therefore, when the impedance method is adopted to detect the white blood cell count value of the blood sample of the cow, the detection result with the dilution ratio of 1200: 1-3500: 1 is more accurate than the detection result with the dilution ratio of 250: 1-500: 1, and when the dilution ratio is in a certain high dilution ratio range, the count value is closer to the actual count value.
Example two:
the cell detection method and the cell analyzer suitable for sheep, goats and cows provided by the embodiment are mainly different from the first embodiment in that: in the first embodiment, the cell analyzer can realize the differential counting detection of white blood cells, and two detection sample solutions are obtained by adopting a method of diluting and dissolving red blood cells twice and are respectively detected, so that the differential counting data of the white blood cells of blood samples of sheep, goats and cows can be accurately detected; in the embodiment, the cell analyzer can realize counting detection of white blood cells, but cannot realize classification detection of white blood cells, and the first detection sample liquid is obtained by adopting a method of diluting and dissolving red blood cells once and is detected, so that the counting data of the white blood cells of blood samples of sheep, goats and cows can be accurately detected.
Specifically, in this embodiment, the cell detection method suitable for sheep, goats, and cows includes a first detection sample liquid preparation step and a first detection step; but not the third detection sample liquid preparation step and the second detection step.
As an embodiment, the cell detection method further includes a sampling step and a first sampling step, and the specific implementation of the sampling step and the first sampling step can refer to the first embodiment, and will not be described in detail herein.
In one embodiment, the cell detection method further comprises a fourth detection sample solution preparation step, a second sample separation step, and a third detection step, wherein the first detection sample solution is prepared by directly diluting, hemolyzing, or preparing a part of the fourth detection sample solution with a diluent and a hemolytic agent. The fourth step of preparing a sample solution for detection, the second step of sampling, and the third step of detection can be implemented by referring to the first embodiment, and will not be described in detail herein.
Specifically, the cell analyzer provided in the present embodiment also includes an analyzer execution main body 100, a human-computer interaction device 200, and a controller 300; the analyzer execution main body 100 is configured to be able to perform the following actions under the control of the controller 300: preparing the blood sample into a first detection sample solution by using a diluent and a hemolytic agent, and performing white blood cell counting detection on the first detection sample solution by using an impedance method to obtain first detection data of white blood cells. That is, in the present embodiment, the analyzer executing main body 100 does not execute the third detection sample liquid and the preparation and detection.
In addition to the above differences, the cell detection method and other parts of the cell analyzer provided in this embodiment can be optimized according to the embodiment, and are not described in detail herein.
Example three:
the cell analyzer provided in this embodiment is different from the first and second embodiments mainly in that the configurations of the human-computer interaction device 200 and the controller 300 are different, and specifically embodied as follows: in the first and second embodiments, the human-computer interaction device 200 is configured to be capable of selecting a first type animal detection mode and a second type detection mode; in this embodiment, the human-computer interaction device 200 is configured to be able to select only the first type of animal detection mode.
Specifically, in the present embodiment, the cell analyzer includes an analyzer execution main body 100, a human-computer interaction device 200, and a controller 300; the analyzer execution body 100 is configured to be able to execute a white blood cell classification detection item and a white blood cell count detection item on the blood sample;
the human-computer interaction device 200 is configured to be capable of selecting a first type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller 300 is configured to: according to the animal detection mode selected by the human-computer interaction device 200, the analyzer execution main body 100 is controlled to perform the following detection on the blood sample: when the animal detection mode selected by the human-computer interaction device 200 is the first type animal detection mode, controlling the analyzer execution main body 100 to dilute the blood sample to a third preset proportion value through the diluent and the hemolytic agent to prepare a third detection sample solution, and controlling the analyzer execution main body 100 to perform leucocyte counting detection on the third detection sample solution by adopting an impedance method to obtain second detection data of leucocytes; controlling the analyzer execution main body 100 to dilute the third detection sample solution to a first preset proportion value through the diluent and the hemolytic agent to prepare a first detection sample solution, and controlling the analyzer execution main body 100 to perform leucocyte counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data of leucocytes, wherein the first detection data comprises a first counting value of the leucocytes; wherein, the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500:1, the third preset proportion value is greater than or equal to 1200:1 and less than a first preset proportional value.
In addition to the above differences, the present embodiment provides other parts of the cell analyzer, which can be optimally designed according to the first embodiment and the second embodiment, and will not be described in detail herein.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (15)
1. A cell analyzer suitable for sheep, goats and cows, which is characterized in that: the system comprises an analyzer execution main body, a human-computer interaction device and a controller;
the analyzer execution body is configured to be capable of performing a white blood cell count detection item on a blood sample using an impedance method;
the human-computer interaction device is configured to be able to select an animal detection mode as follows: the detection device comprises a first type animal detection mode and a second type animal detection mode, wherein the first type animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
controlling the analyzer execution main body to execute the following actions on the blood sample according to the animal detection mode selected by the man-machine interaction device:
when the animal detection mode selected by the human-computer interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute a blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample solution, and controlling the analyzer execution main body to perform leucocyte counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data of leucocytes, wherein the first detection data comprises a first counting value of the leucocytes;
when the animal detection mode selected by the human-computer interaction device is a second animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a second preset proportion value through diluent and hemolytic agent to prepare a second detection sample solution, and controlling the analyzer execution main body to perform leucocyte counting detection on the second detection sample solution by adopting an impedance method;
wherein the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500:1, the second preset proportion value is greater than or equal to 100: 1 and less than or equal to 500: 1.
2. a cell analyzer suitable for use in sheep, goats and cows as claimed in claim 1 wherein: the second type of animal detection mode comprises at least one of a cat detection mode, a dog detection mode and a horse detection mode.
3. A cell analyzer suitable for sheep, goats and cows, which is characterized in that: the system comprises an analyzer execution main body, a human-computer interaction device and a controller;
the analyzer execution body is configured to be capable of performing a white blood cell count detection item on a blood sample using an impedance method;
the human-computer interaction device is configured to be capable of selecting a first type of animal detection mode, wherein the first type of animal detection mode comprises at least one of a sheep detection mode, a goat detection mode and a cow detection mode;
the controller is configured to:
controlling the analyzer execution main body to perform the following actions on the blood sample according to the animal detection mode selected by the human-computer interaction device:
when the animal detection mode selected by the human-computer interaction device is a first type animal detection mode, controlling the analyzer execution main body to dilute the blood sample to a first preset proportion value through diluent and hemolytic agent to prepare a first detection sample solution, and controlling the analyzer execution main body to perform leucocyte counting detection on the first detection sample solution by adopting an impedance method;
wherein the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500: 1.
4. a cell analyzer suitable for sheep, goats and cows as claimed in any one of claims 1 to 3, wherein: the first preset proportion value is greater than or equal to 1500:1 and less than or equal to 3000: 1.
5. a cell analyzer suitable for sheep, goats and cows as claimed in claim 4 wherein: the first preset proportion value is (2400 ± 200): 1.
6. a cell analyzer suitable for sheep, goats and cows as claimed in any one of claims 1 to 3, wherein: the analyzer execution body is further configured to be capable of performing a white blood cell differential test item on a blood sample using an impedance method;
the controller is configured to: when the animal detection mode selected by the man-machine interaction device is the first type of animal detection mode, controlling the analyzer to execute the main body to execute the following actions:
diluting the blood sample to a third preset proportion value through a diluent and a hemolytic agent to prepare a third detection sample solution;
carrying out white blood cell counting detection on at least part of the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells;
diluting at least part of the third detection sample solution to the first preset proportion value by using a hemolytic agent and a diluent to prepare the first detection sample solution;
carrying out white blood cell counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data;
wherein the third preset proportion value is greater than or equal to 1200:1 and less than the first preset proportion value.
7. A cell analyzer adapted for use with sheep, goats and cows as claimed in claim 6 wherein: the controller is further configured to: analyzing and processing the first detection data and the second detection data to obtain the classification counting data of the white blood cells;
the first detection data comprises a first histogram which is a histogram of the number and volume distribution of leukocytes and the first count value of leukocytes; the second detection data comprises a second histogram and a second counting value of white blood cells, and the second histogram is a histogram of the number and volume distribution of red blood cell fragments and white blood cells;
the controller analyzes and processes the first detection data and the second detection data to obtain the differential count data of the white blood cells, and the embodiment of the controller comprises the following steps: calculating S1 and S2 according to the formula S1/(S1+ S2) ═ WBC1/WBC2, and determining the boundary between the red cell debris and the white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram through the boundary to obtain a third histogram; classifying and counting the white blood cells in the third histogram according to the volume to obtain classified counting data of the white blood cells;
wherein S1 is the distribution area of white blood cells in the first histogram, S2 is the distribution area of red blood cell debris in the second histogram, WBC1 is the first count value, and WBC2 is the second count value.
8. A cell analyzer adapted for use with sheep, goats and cows as claimed in claim 6 wherein: the analyzer execution body is also configured to be capable of executing a red blood cell count detection item on a blood sample, and comprises a sampling device, a diluent supply device, a hemolytic agent supply device, a white blood cell count pool, a red blood cell count pool, a first detection device and a second detection device, wherein the first detection device is used for carrying out white blood cell count detection, and the second detection device is used for carrying out red blood cell count detection;
the controller is configured to: when the animal detection mode selected by the man-machine interaction device is the first type of animal detection mode, controlling the analyzer to execute the main body to execute the following actions:
controlling the sampling device to collect a blood sample and adding the collected blood sample into the leucocyte counting pool;
controlling the diluent supply device to add diluent into the white blood cell counting cell so as to dilute the blood sample to a fourth preset proportion value, and preparing a fourth detection sample solution;
controlling the sampling device to collect a first part of the fourth detection sample solution from the white blood cell counting cell, and controlling the sampling device to add at least part of the fourth detection sample solution in the collected first part of the fourth detection sample solution into the red blood cell counting cell for dilution and red blood cell counting detection;
controlling the sampling device to collect a second portion of the fourth test sample solution from the white blood cell counting cell;
controlling to drain the fourth detection sample liquid remaining in the white blood cell counting cell;
controlling the sampling device to add at least part of the fourth detection sample solution in the second collected detection sample solution into the white blood cell counting cell;
controlling the diluent supply device to convey diluent to the white blood cell counting cell, and controlling the hemolytic agent supply device to convey hemolytic agent to the white blood cell counting cell to dissolve red blood cells in the fourth detection sample solution, and diluting the blood sample to a first preset proportion value to prepare a third detection sample solution;
controlling the first detection device to perform white blood cell counting detection on the third detection sample liquid by adopting an impedance method to obtain second detection data of white blood cells;
controlling the diluent supply device to deliver diluent to the white blood cell counting cell, and controlling the hemolytic agent supply device to deliver hemolytic agent to the white blood cell counting cell, so as to dilute the third detection sample solution to the first preset proportion value, and thus obtaining the first detection sample solution;
controlling the first detection device to perform leucocyte counting detection on the first detection sample liquid by adopting an impedance method to obtain first detection data;
and the fourth preset proportion value is smaller than the third preset proportion value.
9. A cell detection method suitable for sheep, goats and cows is characterized by comprising the following steps: the method comprises the following steps:
a sampling step, controlling a sampling device to collect a blood sample in a blood sample container;
a first sampling step of controlling the sampling device to transfer at least a part of the collected blood sample to the leucocyte counting cell;
a first detection sample solution preparation step of controlling a diluent supply device to convey diluent to the white blood cell counting cell, and controlling a hemolytic agent supply device to convey hemolytic agent to the white blood cell counting cell to dissolve red blood cells in the blood sample, and diluting the blood sample to a first preset proportion value to prepare a first detection sample solution;
a first detection step of controlling a first detection device to perform white blood cell counting detection on the first detection sample solution by adopting an impedance method to obtain first detection data of white blood cells, wherein the first detection data comprises a first count value of the white blood cells;
wherein the first preset proportion value is greater than or equal to 1200:1 and less than or equal to 3500: 1.
10. the method of claim 9 for the detection of cells suitable for sheep, goats and cows comprising: the first preset proportion value is greater than or equal to 1500:1 and less than or equal to 3000: 1.
11. the method of claim 10 for the detection of cells in sheep, goats and cows comprising: the first preset proportion value is (2400 ± 200): 1.
12. the method of claim 9 for the detection of cells suitable for sheep, goats and cows comprising: the cell detection method further includes a third detection sample liquid preparation step after the first sample separation step and before the first detection sample liquid preparation step, the third detection sample liquid preparation step including: controlling the diluent supply device to convey diluent to the white blood cell counting cell, and controlling the hemolytic agent supply device to convey hemolytic agent to the white blood cell counting cell to dissolve red blood cells in the blood sample, and diluting the blood sample to a third preset proportion value to prepare a third detection sample solution;
the first detection sample liquid preparation step includes: diluting at least part of the third detection sample solution to the first preset proportion value by adopting a diluent and a hemolytic agent to prepare the first detection sample solution;
the cell detection method further includes a second detection step after the third detection sample liquid preparation step, the second detection step including: controlling the first detection device to perform leucocyte counting detection on at least part of the third detection sample liquid by adopting an impedance method to obtain second detection data;
wherein the third preset proportion value is greater than or equal to 1200:1 and less than the first preset proportion value.
13. The method of claim 12 for the detection of cells suitable for sheep, goats and cows comprising: the cell detection method further comprises a fourth detection sample solution preparation step before the third detection sample solution preparation step, wherein the fourth detection sample solution preparation step comprises: controlling the diluent supply device to convey diluent to the leukocyte counting cell so as to dilute the blood sample to a fourth preset proportion value, and preparing a fourth detection sample solution;
the cell detection method further includes a second sample separation step after the fourth detection sample liquid preparation step, the second sample separation step including: delivering a first portion of the fourth test sample fluid to a red blood cell count cell;
the third detection sample liquid preparation step comprises: dissolving red blood cells in a third part of the fourth detection sample solution by using a diluent and a hemolytic agent, and diluting the third part of the fourth detection sample solution to a third preset proportion value to prepare a third detection sample solution;
and the fourth preset proportion value is smaller than the third preset proportion value.
14. The method of claim 13 for the detection of cells suitable for use in sheep, goats and cows, wherein: the second sampling step comprises the following steps: controlling the sampling device to collect a first part of the third detection sample from the white blood cell counting cell, and controlling the sampling device to add the collected first part of the fourth detection sample liquid into the red blood cell counting cell;
the third detection sample liquid preparation step includes: controlling the sampling device to collect a second part of the third detection sample from the leucocyte counting pool, and controlling to drain the fourth detection sample liquid remained in the leucocyte counting pool; controlling the diluent supply device to deliver a diluent to the white blood cell counting cell to form a bottom solution, controlling the sampling device to add a third portion of the fourth detection sample solution to the white blood cell counting cell, controlling the diluent supply device to deliver a diluent to the white blood cell counting cell, and controlling the hemolytic agent supply device to deliver a hemolytic agent to the white blood cell counting cell to dissolve red blood cells in the blood sample, and diluting the blood sample to the third preset proportion value to prepare the third detection sample solution;
wherein the third test sample volume of the second portion is equal to or greater than the third test sample volume of the third portion.
15. A cell detection method as claimed in any one of claims 9 to 14 suitable for use in sheep, goats and cows, wherein:
the first detection data comprises a first histogram which is a histogram of the number and volume distribution of leukocytes and the first count value of leukocytes; the second detection data comprises a second histogram and a second counting value of white blood cells, and the second histogram is a histogram of the number and volume distribution of red blood cell fragments and white blood cells;
the cell detection method further comprises a data analysis step comprising: analyzing and processing the first detection data and the second detection data to obtain the classification counting data of the white blood cells;
the data analysis step comprises: calculating S1 and S2 according to the formula S1/(S1+ S2) ═ WBC1/WBC2, and determining the boundary between the red cell debris and the white cells in the second histogram; removing the distribution area of the red blood cell fragments in the second histogram through the boundary to obtain a third histogram; classifying and counting the white blood cells in the third histogram according to the volume to obtain classified counting data of the white blood cells;
wherein S1 is the distribution area of white blood cells in the first histogram, S2 is the distribution area of red blood cell debris in the second histogram, WBC1 is the first count value, and WBC2 is the second count value.
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