CN101236195B - Cellanalyzer, method for analyzing body fluid and control system thereof - Google Patents
Cellanalyzer, method for analyzing body fluid and control system thereof Download PDFInfo
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- CN101236195B CN101236195B CN200810005239.2A CN200810005239A CN101236195B CN 101236195 B CN101236195 B CN 101236195B CN 200810005239 A CN200810005239 A CN 200810005239A CN 101236195 B CN101236195 B CN 101236195B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/12—Coulter-counters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G01N2015/012—
-
- G01N2015/014—
-
- G01N2015/016—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1486—Counting the particles
Abstract
The invention provides a kind of cellanalyzer, comprising: mode determination setup unit, for setting humoral determination pattern; Measure and start indicating member, for accepting to start the instruction of mensuration; Optical information acquiring unit, sample is measured in illumination, from the contained cell of this mensuration sample, obtains optical information; Analytic unit, after above-mentioned humoral determination pattern is set, when said determination start indicating member receive start measure instruction time, according to measuring by humoral specimen and leucocyte the above-mentioned optical information of obtaining in the said determination sample of preparing with reagent, the contained cell of this mensuration sample is at least categorized as to the karyocyte beyond leucocyte and leucocyte, to the Other nucleated cells differential count beyond leucocyte and leucocyte. The present invention also provides a kind of method for analyzing body fluid and a kind of control system.
Description
Technical field:
The present invention relates to a kind of can not only measure blood, also can measure cerebrospinal fluid (marrow as sampleLiquid), beyond the blood such as hydrothorax (liquor pleurae) and ascites other body fluid cellanalyzer,Method for analyzing body fluid and control system thereof.
Background technology:
In clinical examination field, conventional cellanalyzer is taking the blood that picks up from health as being inspectedOne of sample is analyzed, and the reference monitoring this analysis result as diagnosis and treatment.
Meanwhile, clinical examination field is thirsted for measuring easily beyond the blood such as cerebrospinal fluidBody fluid. Conventionally in body fluid hardly containing cell, but have tumour and damage when ill or relevant organTime, will find the cells such as hemorrhage (haemocyte) and abnormal cell, bacterium.
On No. 2003/0215890 communique of U.S. Patent Application Publication, describe about thin with bloodThe technology of the cell in born of the same parents' analysis-e/or determining body fluid. U.S. Patent Application Publication accordinglyOn No. 2003/0215890 communique, describe, will contain the reagent of aldehyde, surfactant and cyclodextrinComposition mixes with cerebrospinal fluid (CSF), and formation determination sample, uses ADVIA120 cell analysisInstrument is analyzed made mensuration sample, classifies also according to cytological map shown in Figure 11 A～Figure 11 G of this communiqueCell in counting brain spinal fluid.
But, according to disclosed skill on No. 2003/0215890 communique of U.S. Patent Application PublicationArt, in fact only analyzes cerebrospinal fluid (CSF) as body fluid, about as the body such as ascites and hydrothoraxLiquid is not analyzed. Conventionally in cerebrospinal fluid, scarcely contain haemocyte particle composition in addition, andIn body fluid beyond the cerebrospinal fluid such as ascites and hydrothorax, because of patient disease contain sometimes middle chrotoplast,Macrophage and tumour cell etc. Like this, when using U.S. Patent Application Publication theOn No. 2003/0215890 communique, disclosed technical Analysis contains haemocyte particle composition in additionBody fluid time, just likely cause such as certain cell compartment at cytological map occurs beyond haemocyteParticle composition, cannot draw the problem of Correct Analysis result.
Summary of the invention:
Scope of the present invention, only by appended claim book defined, is not subject in any degreeThe statement of this joint summary of the invention is limit.
The cellanalyzer of the mensuration haemocyte that the present invention the first side provides comprises: measure mouldFormula setup unit, for setting humoral determination pattern; Measure and start indicating member, for acceptingStart the instruction of measuring; Optical information acquiring unit, sample is measured in illumination, from this mensuration sampleIn contained cell, obtain optical information; Analytic unit, after above-mentioned humoral determination pattern is set,When said determination starts indicating member while receiving the instruction that starts to measure, according to by humoral specimen andLeucocyte is measured the above-mentioned optical information of obtaining in the said determination sample of preparing with reagent, shouldMeasure the contained cell of sample and be at least categorized as leucocyte and leucocyte karyocyte in addition, dialogueOther nucleated cells differential count beyond cell and leucocyte.
The cellanalyzer that the present invention the second side provides comprises: mode determination setup unit,Be used for setting humoral determination pattern; Measure and start indicating member, for accepting to start the finger of mensurationShow; Aspirating specimen unit, moves sample for inhaling; Measure sample and prepare unit, use aspirating specimenUnit is inhaled the sample and the leucocyte that move and is measured with reagent formation determination sample; Optical information is obtained listUnit, sample is measured in illumination, from the contained cell of this mensuration sample, obtains optical information; Analysis listUnit, according to the above-mentioned optical information of obtaining, classifies to the contained cell of said determination sample,And to the above-mentioned cell count of classifying. Above-mentioned humoral determination pattern is set single by said determination patternUnit set after, when said determination start indicating member receive start measure instruction time, above-mentioned surveyDetermining sample prepares unit and just inhales by above-mentioned aspirating specimen unit the humoral specimen and the above-mentioned leucocyte that moveMeasure with reagent and prepare said determination sample, above-mentioned analytic unit is according to obtaining from said determination sampleThe above-mentioned optical information of getting is leucocyte and leucocyte by measuring contained cell classification in sampleCell in addition, and to the Other nucleated cells differential count beyond leucocyte and leucocyte.
The method for analyzing body fluid that the present invention the 3rd side provides comprises: (a) step: set and measurePattern, to set humoral determination pattern; (b) step: humoral determination pattern connects after settingStarted the instruction of measuring; (c) step: accept, after the above-mentioned instruction that starts to measure, to use illuminationPenetrate by humoral specimen and leucocyte and measure the mensuration sample of preparing with reagent, from this mensuration sample instituteObtain optical information containing in cell; (d) step: according to the above-mentioned optical information of obtaining, by upperState the contained cell of mensuration sample and be at least categorized as leucocyte and leucocyte karyocyte in addition, and rightOther nucleated cells differential count beyond leucocyte and leucocyte.
The present invention the 4th side provides a kind of control system of body fluid analysis instrument, comprising: a kind of surveyMould-fixed setting control system, sets mode determination, to set humoral determination pattern; A kind ofMeasure instruction and accept control system, after humoral determination pattern is set, accept to start the instruction of mensuration;A kind of optical information is obtained control system, accepts, after the above-mentioned instruction that starts to measure, to use irradiationMeasure by humoral specimen and leucocyte the mensuration sample of preparing with reagent, contained from this mensuration sampleIn cell, obtain optical information; And a kind of cell classification and counting control system, according to what obtainAbove-mentioned optical information, is at least categorized as leucocyte and leucocyte by contained said determination sample cellKaryocyte in addition, and to the Other nucleated cells differential count beyond leucocyte and leucocyte.
Brief description of the drawings:
Fig. 1 is the outside drawing of the cellanalyzer of an embodiment of the present invention.
Fig. 2 is the block diagram of analysis-e/or determining device.
Fig. 3 is the block diagram of fluid device.
Fig. 4 is the optical system demonstration figure of leucocyte detector.
Fig. 5 is the demonstration figure of RBC/PLT detector.
Fig. 6 is the demonstration figure of HGB detector.
Fig. 7 is that sample is measured the flow chart of processing.
Fig. 8 is the accompanying drawing of the display frame for setting mode determination.
Fig. 9 is the flow chart of presequence processing.
Figure 10 is the ideograph of measuring the scatter diagram of the DIFF mensuration sample of being prepared by body fluid.
Figure 11 is the cellanalyzer measurement result of embodiment and antithetic measurement resultComparison diagram.
Figure 12 is the ideograph of measuring the scatter diagram of the DIFF mensuration sample of being prepared by blood.
Figure 13 is the display frame of the measurement result of blood measuring pattern.
Figure 14 is the display frame of the measurement result of humoral determination pattern.
Figure 15 is the display frame of the measurement result of humoral determination pattern.
Figure 16 is the display frame of the measurement result of humoral determination pattern.
Figure 17 is the blank confirmation picture detecting of beginning shown under humoral determination pattern.
Detailed description of the invention:
The specific embodiment of the present invention is below described with reference to the accompanying drawings.
Figure 1 shows that cellanalyzer 1. This cellanalyzer 1 is as examining for bloodThe multinomial Automatic Blood Cell Analyzer of looking into, can be to the inner blood of specimen container (heparin tube)Sample is measured, and obtains the characteristic information that represents contained haemocyte feature in sample, and to thisCharacteristic information carries out analyzing and processing. This cellanalyzer 1 can also analysing body fluid. In this realityExecute the cellanalyzer of mode, analytic target body fluid refer to beyond blood be present in endoceliacCoelomic fluid. Specifically refer to cerebrospinal fluid (marrow liquid, CSF: the hydrops of the ventricles of the brain and subarachnoid space),Hydrothorax (liquor pleurae, PE: pleural effusion), ascites (seroperitoneum), the pericardium liquid (chambers of the heartHydrops), joint fluid (synovia: the liquid in joint, bursa synovialis and stndon sheath) etc. Peritonaeum is saturatingDislysate and the abdominal cavity cleaning liquid inside etc. of analysing (CAPD) also can be used as the one of body fluid and dividesAnalyse. Conventionally in these liquid, almost there is no cell, but have tumour and be subject to when ill or relevant organWhen damage, just may contain the cells such as haemocyte, abnormal cell and bacterium. Such as cerebrospinal fluid,From analysis result, can make following clinical deduction. Such as, if red blood cell increases, mayBe subarachnoid hemorrhage, if neutrophil cell increases, suspicious is meningitis, if acidophilia is thinBorn of the same parents increase, and can suspect and suffer from infectious diseases (parasite and fungi), if monocyte increasesAdd, can suspect for Tuberculous meningitis and viral meningitis, if other cells increase,Can suspect and shift to meninges for tumour. As for ascites and hydrothorax etc., if dehematize extracellular is also containedThe karyocytes such as middle chrotoplast, macrophage and tumour cell, just can be used as diseases such as suspecting cancerSick index, by analyzing the karyocyte beyond this haemocyte, can obtain these indexs.
Cellanalyzer 1 is by measuring as the blood of sample and the determinator of body fluid 2 HesThe data processing dress of analysis result is processed, obtained to the measurement result that determinator 2 is exportedPut 3 formations. Data processing equipment 3 comprises controller 301, display 302 and input equipment303. In Fig. 1, determinator 2 respectively exists as a device with data processing equipment 3,Also can unite two into one as a device.
Fig. 2 is the block diagram of the determinator 2 of cellanalyzer 1. As shown in Figure 2, measureDevice 2 comprises haemocyte test section 4, output signal (analog signal) to test section 4Analogue signal processor 5, microcomputer 6, demonstration operation part 7 and the mensuration blood processedDevice for mechanical part 8 with body fluid. Device for mechanical part 8 comprises following fluid device 81.
Fig. 3 is the structured flowchart of fluid device 81. As shown in Figure 3, fluid device 81 comprisesAspirating specimen mouth 18, several reagent container 11, sampling valve 12 and reaction warehouse 13～17. SampleSuction moves mouth 18 and inhales and move sample from specimen container, and this sample is sent to sampling valve 12. Sampling valve 12The sample of importing is divided into a certain amount of some deciles. This Segmentation Number is because of mode determination (each surveyMould-fixed) and different, measure red blood cell number, leukocyte count, platelet count and blood red eggThe CBC type specimen of white concentration is divided into trisection. On above-mentioned CBC mode determination, add againThe CBC+DIFF pattern that leucocyte five is classified, sample quadrisection. At CBC+DIFFIn mensuration project, add again the CBC+DIFF+RET pattern of measuring granulophilocyte, be divided into five etc.Point. Equally, in the mensuration project of CBC+DIFF pattern, add again erythroblast and measure projectCBC+DIFF+NRBC pattern is also that sample is divided into five deciles. In CBC+DIFF patternIn+RET mensuration project, add again the CBC+DIFF+RET+NRBC that measures erythroblastPattern, is divided into six deciles. Above mode determination is the blood measuring pattern of measuring blood entirely.Finally, in the humoral determination pattern of mensuration body fluid, sample is divided into bisection.
Reagent (dilution) imports this sampling valve 12, the sample of cutting apart from reagent container 11Decile is transported to reaction warehouse 13～17 and aftermentioned HGB detector 43 together with reagent. Reaction warehouse13 by a certain amount of sample (decile) that provides sampling valve 12 to extract without illustrated constant displacement pump,A certain amount of dilution and a certain amount of dyeing liquor, these samples and reagent, through mixing, are prepared whiteThe classify mensuration sample of (DIFF) use of cell four.
As dilution, the reagent " leucocyte that can suitably use SYSMEX Co., Ltd to provideHemolysin STROMATOLYSER-4DL ". This reagent contains surfactant, canLysed erythrocyte. The reagent that dyeing liquor can suitably use SYSMEX Co., Ltd to provide is equally " whiteThe cell four liquid STROMATOLYSER-4DS that classifies ". This dyeing liquor contain ethylene glycol,Low alcohol, polymethine, after above-mentioned dilution haemolysis, blood cell composition is colored, and finally makesGo out 50 times of dilution samples.
If select body fluid mode determination, with leucocyte four classification therewith with measuring samplesThe condition of same specimen amount, same reagent and same reagent amount is prepared leukocyte differential count with measuring examinationSample. But as described later, the leukocyte differential count leucocyte of humoral determination pattern is not four classes,But two classes.
Reaction warehouse 14 by a certain amount of sample that provides sampling valve 12 to gather without illustrated constant displacement pump,A certain amount of dilution hemolytic agent and a certain amount of dyeing liquor, by these samples and reagent mix, be manufactured withNucleated red blood cell (NRBC) is measured with measuring sample.
Reaction warehouse 15 by a certain amount of sample that provides sampling valve 12 to gather without illustrated constant displacement pump,A certain amount of dilution hemolytic agent and a certain amount of dyeing liquor, by these samples and reagent mix, make netKnitting red blood cell (RET) measures with measuring sample.
Reaction warehouse 16 by a certain amount of sample that provides sampling valve 12 to gather without illustrated constant displacement pump andA certain amount of dilution hemolytic agent, by these samples and reagent mix, making leucocyte and basophilla are thinBorn of the same parents (WBC/BASO) measure with measuring sample.
Reaction warehouse 17 is by a certain amount of sample, that provides sampling valve 12 to gather without illustrated constant displacement pumpQuantitatively dilution, by these samples and reagent mix, makes red blood cell/blood platelet (RET/PLT)Measure with measuring sample.
In addition, after a certain amount of sample that sampling valve 12 gathers, a certain amount of dilution hemolytic agent are also suppliedState HGB detector 43.
There is the leukocytic leucocyte detector 41 of detection test section 4. This leucocyte detector 41Also for the detection of erythroblast and granulophilocyte. Test section 4 is except leucocyte detectorOutside 41, measure in addition the RBC/PLT detector 42 of red blood cell number and platelet count and measureThe HGB detector 43 of hemochrome amount in blood.
Above-mentioned leucocyte detector 41 is mainly made up of fluorescence detector, particularly, and by usingThe detector of flow cytometry forms. At this, so-called cell art measures cell and other biologicalLearn physical property and the chemical property of particle, so-called flow cytometry refers to: allow these particles from carefullyIn stream by, carry out method for measuring. Figure 4 shows that the optical system of leucocyte detector 41.In the figure, the light beam penetrating from lasing fluorescence diode 401 is irradiated to by collimating mirror 402The haemocyte of flowing through in sheath flow pool 403. This leucocyte detector 41 detects in sheath flow pool 403The forward scattering luminous intensity sent under Ear Mucosa Treated by He Ne Laser Irradiation of haemocyte, lateral scattering luminous intensity andSide direction fluorescence intensity, using this as haemocyte characteristic parameter.
At this, scattering of light is because this particle of haemocyte becomes on the direct of travel of lightBarrier, therefore light change the phenomenon that its direct of travel produces. Detect this scattered light passableObtain the particle characteristics information about particle size and composition. Forward scattering only refers to that particle sendsWith the essentially identical scattered light of direct of travel of institute's irradiation light. Can obtain from forward direction scattered lightMust be about the characteristic information of particle (haemocyte) size. Lateral scattering only particle send withInstitute's irradiation light scattered light slightly in vertical direction. Can obtain relevant particle from side scattered lightInner characteristic information. When Ear Mucosa Treated by He Ne Laser Irradiation is on haemocyte particle time, lateral scattering luminous intensity is gotCertainly in the complexity (quantity of shape, size, density and the particle of core) of cell interior. CauseThis, utilize this characteristic of lateral scattering luminous intensity (discriminating) haemocyte of can classifying, and surveyDetermine haemocyte quantity. In addition, already described present embodiment has been taked use forward scattering light and side directionScattered light is as the structure of scattered light, but is not limited to this, as long as can obtain analyze requiredThe scattered light signal of reflection particle characteristics, scattered light sees through for light source the light that sheath flow pool irradiatesThe angle of optical axis do not limit.
Illumination, such as that fluorescent material of dyed haemocyte, is sent than shone optical wavelengthLonger light. Fluorescence intensity is to dye more good byer force, measures this fluorescence intensity and just can obtain relevantThe characteristic information of blood cell staining degree. Therefore, poor according to (side direction) fluorescence intensity, canThe mensuration such as leucocyte is classified to.
As shown in Figure 4, the flow through haemocyte (leucocyte and erythroblast) of sheath flow pool 403By light emitting diode, (forward direction is loose by condenser 404 and pin hole 405 for the forward scattering light sendingPenetrate light optical collector) 406 acceptance. Side scattered light is by condenser 407, dichronic mirror 408, lightLearning film 409 and pin hole 410 is accepted by photomultiplier (side scattered light optical collector) 411.Side direction fluorescence by condenser 407 and dichronic mirror 408 by photomultiplier (side direction fluorescence light harvestingDevice) 412 acceptance. From the suffered optical signal difference of each optical collector 406,411 and 412 outputsAmplify and ripple by the analogue signal processor 5 being formed by amplifier 51,52,53 etc.After the analog signal processing such as shape processing, be transported to microcomputer 6.
Below, describe with regard to the structure of RBC/PLT detector 42. Fig. 5 is RBC/PLTThe brief configuration ideograph of detector 42. RBC/PLT detector 42 can use sheath stream DCDetection method is measured red blood cell number and platelet count. RBC/PLT detector 42 has as shown in Figure 5Sheath flow pool 42a. This sheath flow pool 42a is provided with the adding mouth 42b of upward opening, and sample can be fromReaction warehouse 17 adds this adding mouth 42b. Sheath flow pool 42a also has upwards tapered taper sampleStorehouse 42c, above-mentioned adding mouth 42b is just configured in the central interior of this sample storehouse 42c. Sample storehouse 42cUpper end is provided with hole 42d, and this hole 42d is just in time relative with adding mouth 42b center. Provide for sample deviceMensuration sample upwards transport from the front end of adding mouth 42b, meanwhile, front sheath fluid is fed toSample storehouse 42c, front sheath fluid upwards flows to hole 42d. At this, measure the bag of sample at front sheath fluidEnclose current downflow, taper sample storehouse 42c makes to measure sample stream and attenuates, measure haemocyte in sample byIndividual by hole 42d. Hole 42d establishes electrode, between this electrode, is provided with DC current. Detect when measuringWhen sample stream via hole 42d, the variation of the D.C. resistance of hole 42d, outputs to control by this signal of telecommunicationDevice 25. Above-mentioned D.C. resistance can increase during by hole 42d at haemocyte, therefore, and this telecommunicationsNumber reflection haemocyte is by the information of hole 42d, by this signal of telecommunication is carried out to signal processing,To red blood cell and platelet count.
The top of hole 42d is provided with the recovery tube 42e extending up and down. This recovery tube 42e configurationIn the sample storehouse 42f connected by 42dYu Yang storehouse, hole 42c. Yu Yang storehouse, recovery tube 42e lower end42f inwall separates. Sample storehouse 42f has rear sheath fluid to provide, and after this sheath fluid is along the recovery tube of sample storehouse 42fThe exterior lateral area of 42e flows downward. Flowing through from recovery tube 42e outside, sheath fluid arrives sample storehouseBehind 42f lower end, between the inwall by recovery tube 42e lower end He Yang storehouse 42f, flow to recovery tube42e inside. Therefore can prevent from refluxing by the haemocyte of hole 42d, thereby prevent haemocyteFlase drop.
Describe with regard to the structure of HGB detector 43 below. HGB detector 43 can pass throughSLS hemoglobin method is measured hemochrome amount (HGB). Fig. 6 is that HGB detector 43 is tiedThe oblique view of structure. HGB detector 43 has the sample pond 43a of dress dilution sample, to sample pond 43aLuminous light emitting diode 43b and reception see through the light collecting element 43c of the transmitted light of sample pond 43a.The quantitative blood of sampling valve 12 is diluted liquid and certain hemolytic agent dilution, preparation by certain dilution rateDilution sample. This hemolytic agent has the hemoglobin in blood is converted to SLS mono-hemoglobinCharacter. This dilution sample feeds to sample pond 43a, deposits in sample pond 43a. Under this state,Make light emitting diode 43b luminous, transmitted light is by relative with light emitting diode 43b every sample pond 43aThe light collecting element 43c of configuration receives. Light emitting diode wavelength light that 43b sends out is easy to by SLS mono-Hemoglobin absorbs, and sample pond 43a is made up of the high plastic material of light transmission, therefore, and light harvestingWhat element 43c received is that light emitting diode 43b utilizing emitted light is only diluted the transmission after sample absorbsLight. Light collecting element 43c will transport to microcomputer 6 with the corresponding signal of telecommunication of light harvesting amount (absorbance), micro-Machine 6 by this absorbance with in advance measure only dilution dulling luminosity ratio, calculate hemoglobinValue.
Microcomputer 6 has the analog signal that analogue signal processor 5 is provided and is converted to data signalA/D converter 61. The output valve of A/D converter 61 is transported to the exerciser 62 of microcomputer 6,Calculate at exerciser 62, light harvesting signal is carried out to certain processing. Exerciser 62 basesThe output valve of test section 4 is made distributed data, and (two-dimentional scatter diagram (unfiled) and one dimension are straightSide figure).
Microcomputer 6 comprises by controlling with processor and controlling with processor and move and form with memoryController 63 and the number being formed with the memory of processor and analysis processor operation use by analysisUnit 64 according to one's analysis. Controller 63 is for controlling by automatically supplying (illustrating for sample device of heparin tubeOmit), instrument mechanical part 8 and other parts of the composition such as the fluid system of sample preparation and mensuration use.Data analysis unit 64 is for carrying out the analyzing and processing such as examination to each distributed data. Analysis result is logicalCross interface 65 and be transported to outside data processing equipment 3, show data display and storage dataDeng processing.
Microcomputer 6 has and shows the interface 66 that operation part 7 is connected and be connected with device for mechanical part 8Interface 67. Exerciser 62, controller 63 and interface 66,67 are connected by bus 68, controlDevice 63 is connected by bus 69 with data analysis unit 64. Show in operation part 7 and comprise behaviourAs personnel assign start to measure the beginning switch of instruction and display instrument state, various setting value withAnd analysis result, accept the touch-screen type liquid crystal display of operating personnel input.
Describe with regard to the operation of the cellanalyzer 1 of present embodiment below. Fig. 7 is this realityExecute the flow chart of the sample analyzer operation of mode. User (operating personnel) connects haemocyte and dividesAnalyse the power supply (step S1) of instrument 1, start cellanalyzer 1. This cellanalyzer 1 startsTime first carry out self-inspection (step S2). In self-inspection, not only test microcomputer 6, check that haemocyte dividesAnalyse the ruuning situation of each operation mechanical part of instrument 1, also measure the not blank sample containing sampleBlank detect. Then, microcomputer 6 carries out initial setting (step S3) to mode determination. At the beginning of thisThe setting value that begins is CBC+DIFF pattern. Particularly, in the processing of step S3, set and surveyDetermine the parameter (service condition) of blood, as reaction warehouse used and minute setting etc. So,The sample analyzer of present embodiment is taking blood measuring pattern as initial launch pattern. Accordingly, bloodCytoanalyze 1 is in accepting to start the holding state of mensuration. Microcomputer 6 is on liquid crystal displayThe picture (step S4) of display notification holding state.
Under this holding state, operating personnel can show operation part 7 switching mensuration by operationPattern. Fig. 8 is the input image mode figure that sets mode determination. This picture Zhong Youbiao this shop 120,Sample is put into schema category 121, subitem detects (mode determination) kind 122 and species of samples 123Each display frame. Sample is put into pattern and is provided with three kinds of patterns: operating personnel manually hold sampleDevice insertion aspirating specimen mouth 18 carries out the manual mode of aspirating specimen; Operating personnel are in advance by sampleInhale the Trace Blood of moving this mensuration sample with reagent mix formation determination sample, with aspirating specimen mouth 18Pre-dilution mode; The closed mode of sample is provided by the conveyer of automatic conveying specimen container.As species of samples, be provided with normal blood sample routine (Normal), (hematopoiesis ancestral is thin for HPCBorn of the same parents) sample (HPC) and body fluid (BodyFluid). Operating personnel can specify respectively sample to putEnter pattern, mode determination and species of samples. If operating personnel specify blood measuring pattern,Species of samples is appointed as to routine (Normal), then specifies any sample to put into pattern and mensurationPattern. If specify humoral determination pattern, operating personnel specify " hand in the pattern of putting into respectivelyDynamic model formula ", subitem detect in specify " CBC+DIFF ", " CBC+DIFF+RET ",One of in " CBC+DIFF+NRBC " and " CBC+DIFF+NRBC+RET ",In species of samples, specify body fluid (BodyFluid). In step S4, operating personnel so refer toFixed desirable mode determination. If operating personnel do not change the mode determination of initial setting and enterThe words of row blood measuring (selecting N at step S5), assign to start to measure and refer to by starting switchShow. Microcomputer 6 receives and starts to measure instruction (step S6), inhales and moves blood preparation from aspirating specimen mouth(step S7).
After blood preparation is inhaled and moved, sample is imported into sampling valve 12 as mentioned above, according to mode determinationSubitem detect kind modulation and measure needed sample (step S14). Then implement to measure examinationThe mensuration operation (step S16) of sample. Such as, when subitem detection kind is set as " 7 ", systemIt is various that standby HGB, WBC/BASO, DIFF, RET, NRBC, RBC/PLT useMeasure sample. Then by leucocyte detector 41 to WBC/BASO, DIFF, RET, NRBCMeasure with mensuration sample, by RBC/PLT detector 42, RBC/PLT is used and measures sampleMeasure, by HGB detector 43, HGB is measured with mensuration sample. Now, thin in vainBorn of the same parents' detector 41 is only provided with one, therefore, and NRBC, WBC/BASO, DIFF, RETEach sample of measuring imports white by the order of NRBC, WBC/BASO, DIFF, RET successivelyCell detection device 41, measures item by item. In service in this mensuration, exerciser 62 is drawn particle and is dividedButut (scatter diagram, histogram). At this, just measure gained optical information according to DIFF and drawThe process of scatter diagram describes. Exerciser 62 with DIFF measure in by leucocyte detector 41Side scattered light and side direction fluorescence signal in the light harvesting signal of output are characteristic parameter, draw twoDimension scatter diagram (distribution of particles figure). This scatter diagram (hereinafter referred to as DIFF scatter diagram) is with side directionScattered light intensity is X-axis, draw the general " red blood cell that occurs taking side direction fluorescence intensity as Y-axisBlood shadow population ", " lymph population ", " monokaryon population ", " neutrophilia+basophillaPopulation " and " acidophil granules subgroup ". These population are passed through place by data analysis unit 64Reason DIFF scatter diagram is identified.
Then, carry out analyzing and processing (step S18) according to measuring gained distribution of particles figure. At thisIn analyzing and processing, the data analysis unit 64 dialogue cell detection devices 41 of microcomputer 6 are measured DIFF and are surveyedThe DIFF scatter diagram that while determining sample, exerciser 62 is drawn is categorized as: four leucocyte groups shown in Figure 12(lymphocyte populations, monocyte group, neutrophilia+basicyte group and acidophic cell group)With erythrocyte ghost group. In the analyzing and processing of present embodiment, that divides from scatter diagram is eachDistance between particle and the position of centre of gravity of each group can obtain each particle to the degree of membership of each group.Be divided into each group according to the each particle of these degree of membership. This method for classifying particles is openly flat in patentBecome on 5-149863 communique and be documented. Measure gained scatter diagram at WBC/BASOUpper, be categorized as basicyte group, basicyte leucocyte group and erythrocyte ghost group in addition.Process according to DIFF Discrete point analysis result (the reference figure that leucocyte four is classified and counted again12) and WBC/BASO Discrete point analysis process the result that leucocyte two is classified and counted, rightThe contained leucocyte of blood preparation carries out five classification. Particularly, data analysis unit 64 is from DIFFDiscrete point analysis is processed gained " blood cell count of neutrophil(e) cell+basicyte " and is deductedWBC/BASO Discrete point analysis is processed gained " blood cell count of basicyte ", Ji KefenDo not draw neutrophil's blood cell count and the blood cell count of basicyte. Accordingly, thin in vainBorn of the same parents are by five classification (lymphocyte, monocyte, neutrophil(e) cell, basicyte and have a liking for acidSexual cell), obtain every blood cell count. In addition,, in RBC/PLT measures, detect rootThe histogrammic curve valley of one dimension that the characteristic information recording according to detector 42 is drawn, to red blood cellClassify with blood platelet. The analysis result of so obtaining outputs to the display of data processing equipment 3On 302 (step S20).
On the other hand, be body if microcomputer 6 receives appointment mode determination as mentioned above at step S5When the input of liquid mode determination, set the parameter (service condition) of carrying out humoral determination as usedReaction warehouse, minute setting etc. (step S8). In the present embodiment, minute asRear described while being blood measuring three times.
Mode determination switches to body fluid by other mode determinations (in this case blood measuring pattern) and surveysWhen mould-fixed (step S9), determinator 2 starts presequence processing (step S10). Before thisSeries processing is to prepare for humoral determination. What under humoral determination pattern, measure is blood cell compositionThe sample that concentration is low, therefore, from blood measuring pattern (being shown as " 1: routine " among Fig. 8)Switch while being set as humoral determination pattern and will carry out presequence processing, to guarantee that background can not affectTo humoral determination result.
Presequence processing comprises blank detection. Blank in series processing before this detects judgement markThe accurate blank than carrying out in determination of blood cell pattern detects (such as cleaning after switch power supply and automaticallyAfter carry out) criterion stricter, the value of setting is for number is below/mono-. In addition establish,While determining to switch to blood measuring pattern by humoral determination pattern, because background affects (residueImpact) generally do not involve blood measuring result, so processing, a presequence do not carry out. At body fluidWhile repeatedly measuring humoral specimen in mode determination, because conventionally can not be subject to the impact of background, instituteNot carry out presequence processing yet. But what humoral specimen also had contains a large amount of particles, therefore,In the time that humoral specimen analysis result exceeds certain value, on interface, there will be " measurement result is too high,Likely affect next sample and measure, will carry out blank and detect. Please by " confirmation ". " etc. letterBreath, notifies operating personnel likely to affect analyzing specimen result below. Operating personnel are by " trueRecognize " button, can carry out blank and detect. Now, interface is provided with " termination " button, behaviourDo personnel as long as press " termination " button, also can not carry out blank and detect, move to standby interface.Detect if do not carry out blank, the best symbol low to measurement result Label reliability. Like thisDo can only be defined in necessary time and append blank detection, with the wave of the time of preventing and reagent classTake.
Fig. 9 be mode determination in the time that blood measuring pattern switches to humoral determination pattern, implementThe flow chart of series processing step. Cellanalyzer 1 is by measuring blank examination at determinator 2Sample is implemented blank detect (step S31), and microcomputer 6 is measurement result and certain feasible value comparison,Judge that whether measurement result is lower than feasible value (step S32). When measurement result is during lower than feasible value,Microcomputer 6 finishes presequence operation, Recovery processing. In the time that measured value is greater than feasible value, microcomputer 6 is sentencedDisconnected blank detect (the step S33) of stipulated number (such as three times) that whether carried out, if blankDetect number of times and do not reach stipulated number, return to step S31, again real in afore mentioned rules number of timesExecute blank detection. If blank detection assay result is not yet lower than feasible value in stipulated number,In demonstration operation part 7, show blank determination result and comprise " confirmation " button, " skyWhite detection " button, " automatically cleaning " button be at interior picture (step S34). If operationPersonnel press " confirmation " button (step S35), and microcomputer 6 finishes presequence operation, recovery placeReason. If press " blank detection " button (step S36), processing is turned back to step by microcomputer 6Rapid S31 again carries out blank and detects. If press " automatically cleaning " button (step S37),Microcomputer 6 is implemented automatically to clean rear (step S38) with special cleaning, and processing is turned back to stepS31, again carries out blank and detects.
After above-mentioned presequence processing finishes, cellanalyzer 1 returns to holding state (step S11).When operating personnel start humoral determination, the same during with the manual mensuration of blood preparation, will measure dressPut 2 aspirating specimen mouth 18 and insert the humoral specimen in specimen container, by starting switch. Microcomputer 6Receive and start to measure after instruction (step S12) and start to inhale and move humoral specimen (step S13).
After humoral specimen is inhaled and moved, humoral specimen the same as blood preparation is imported into sampling valve 91. ByReaction warehouse 13 is prepared RBC/PLT and is measured sample (step S15). Then, detected by leucocyteDevice 41 is measured DIFF and is measured sample, measures RBC/PLT measure examination by RBC/PLT detector 42Sample (step S17). Under the state of humoral determination pattern, measure at leucocyte detector 41Be only DIFF mensuration sample, therefore, even if minute is than the minute of blood measuring patternLonger, also may be than blood measuring time, in shorter time, complete mensuration. So, by by body fluidThe minute of measuring extends longlyer than the minute of blood measuring, can improve particle concentrationThe analysis precision of low humoral specimen. Minute is longer, and the population of counting will be more,Measuring precision will improve. But minute is long, sample disposal ability can decline, withTime by measuring limited in one's ability to the syringe pump of leucocyte detector 41 of sample delivery, therefore with 2～6Be doubly appropriate. In the present embodiment, the minute during by humoral determination pattern is set as bloodWhen mode determination 3 times.
On the other hand, RBC/PLT mensuration sample is all to import resistance-type under any mode determinationDetector 41 is measured under certain a fluid stream condition. Then according to measuring gained characteristic informationCarry out analyzing and processing (step S19), analysis result outputs to the display of data processing equipment 3302 (step S21). In analyzing and processing under blood measuring pattern, analyze the loose point of DIFFFigure etc., calculate five kinds of leucocyte subclass (neutrophil cell: NEUT, lymphocyte: LYMPH,Monocyte: MONO, acidophil: EO, basocyte: BASO) information (numberAmount and ratio), but in analyzing and processing under humoral determination pattern, because blood cell count sometimesSeldom or be subject to breakage, therefore, taking the formal classification of part integration, as two kinds of subclass, (monokaryon is thinBorn of the same parents: MN, apocyte: PMN). Lymphocyte and monocyte belong to monocyte,Neutrophil(e) cell, acidophic cell and basicyte belong to apocyte. This sorting algorithmIdentical with algorithm illustrated in analyzing and processing under blood measuring pattern, description will be omitted.
Then, the analysis result of obtaining at step S19 and feasible value (determine threshold value) are compared(step S22). During detecting, blank in the presequence processing of this feasible value and step S10 makesWith feasible value be same value. In the time that analysis result is greater than feasible value (choosing " Y " in step S22),In step S23, show the confirmation interface 151 that starts blank detection shown in Figure 17. This confirms boundaryOn face 151, show: show that " measurement result is too high, and likely impact sample is below measured.To carry out blank detects. Please by " confirmation ". " information demonstration place 152, the ACK button of information153 and cancel button 154. Next, judge being ACK button 153 or getting of user inputThe button 154 (step S24) that disappears, if input is that (in step S24, choosing " really for ACK buttonRecognize "), implement blank detect (step S25). When the analysis result of obtaining at step S19While being less than feasible value (at step S22 choosing " N ") or input be cancel button time (in stepChoosing " cancellation " in S24), do not carry out blank and detect, return to the processing of step S5.
In body fluid samples, exist sometimes abnormal particle beyond haemocyte (macrophage and inChrotoplast, tumour cell etc.). In cerebrospinal fluid, exist the situation of these abnormal particles rarely found,But more common in other body fluid hydrothorax and ascites. Therefore, no matter body fluid kind how, essenceReally the haemocyte in body fluid classified and counted, will get rid of the shadow of these abnormal particlesRing. Therefore, the present invention is based on abnormal particle and appear at the DIFF scatter diagram of this cellanalyzerThis new knowledge of upside, can measure more accurately as in the body fluid samples of target instrumentLeucocyte. This point is not consider in aforesaid conventional art.
Figure 10 be present embodiment cellanalyzer 1 under humoral determination pattern, measure, analyze byBody fluid and leucocyte are measured the DIFF for preparing with reagent and measure the mould of the scatter diagram that sample obtainsFormula figure. The longitudinal axis of scatter diagram represents side direction fluorescence intensity (more top, fluorescence intensity is stronger),Transverse axis represents lateral scattering luminous intensity (more keep right, scattered light intensity is stronger). Scatter diagram glimmeringThe weak region LF of luminous intensity is distributed with the erythrocyte ghost Gc that haemolysis produces, fluorescence intensity Qiang districtTerritory HF is distributed with the abnormal particles such as middle chrotoplast, and zone line MF is distributed with monocyteMc, multinuclear leucocyte Pc. Therefore, in the analysis of scatter diagram, to be distributed in except region LFWith the particle composition of region MF beyond HF be that leucocyte is analyzed, be categorized as above-mentioned two classes,And counting. In addition, comprise lymphocyte and monocyte in monocyte Mc, multinuclear is thin in vainIn born of the same parents Pc, comprise neutrophil cell, acidophic cell and basicyte.
So when the leucocyte in analysing body fluid, also sometimes in body fluid contained blood cell count seldom orImpaired, therefore as significant information clinically, by leukocyte differential count be monocyte andMultinuclear leucocyte is counted.
In addition, in body fluid, sometimes there is haemocyte abnormal particle (macrophage and middle skin in additionCell, tumour cell etc.). In cerebrospinal fluid, exist the situation of these abnormal particles rarely found, butMore common in other body fluid hydrothorax and ascites. In the scatter diagram of Figure 10, this leucocyte withOuter karyocyte is distributed in region HF. In the present embodiment, can be by beyond leucocyteKaryocyte and leukocyte differentiation, therefore, even if contain having beyond this leucocyte in body fluidNucleus also can be obtained correct leukocyte count. By the cell that appears at region HF is enteredRow counting, the degree that can provide abnormal cell to occur. In the present embodiment, according to differentiationEach cell is divided into region LF, MF and HF by the threshold value in each region, also can manual changeThis threshold value.
Figure 11 is the appropriateness for showing above-mentioned Discrete point analysis method, and relatively adopts this enforcement sideThe cellanalyzer 1 income analysis result of formula and the accompanying drawing that adopts counter point gained count results.Tested sample is hydrothorax, and " this law " in figure represents the cellanalyzer 1 of present embodimentThe leukocyte count (WBC) calculating and other abnormal populations (Others), " Ref "Represent counter point (cell count pond direct counting method (Fuchs-Rosenthal plate) and siteSpin method) result that calculates. Example 1,2,3 is all to analyze to have a large amount of appearance of abnormal particleThe result of hydrothorax, can find out, the cellanalyzer 1 income analysis result of present embodimentAnd between counter point, there is dependency relation.
Figure 13 shows as the above-mentioned DIFF being prepared by the blood analysis result of measuring samplePicture 100 on data processing equipment 3 displays 302. Demonstration is arranged at the top of picture 100The mark this shop viewing area of mark this shop 101, its side is provided with the attribute display district that shows patient's attribute.Attribute display district specifically show mark this shop, patient ID, patient's name, birthdate,Sex, ward, the doctor in charge, mensuration date, minute and remarks etc. Attribute displayBottom, district is provided with the measurement result viewing area that shows measurement result. Measurement result viewing area is by several pagesForm, these pages can be by selecting several labels 102 to carry out display frame. Label is for homepageFace, chart picture and sundry item have several. Demonstration picture when Figure 12 is the selection of chart labelFace. The left-half of measurement result viewing area is provided with the measured value of the measured value that shows measurement resultThe chart viewing area 104 of viewing area 103 and demonstration chart, right half part is provided with and shows mensuration knotThe distribution map viewing area 105 of the distribution map of fruit. The demonstration of measured value viewing area WBC, RBC,Project, data and the units such as NEUT#, BASO#, NEUT%, BASO%, chart is aobviousShow that district 104 shows can be used as in clinical examination about WBC, PLT, RBC or RETThe mark result that sample is abnormal and disease is suspected of useful information.
Distribution map viewing area 105 shows six distribution maps. The scatter diagram of upper left quarter is that DIFF usesScatter diagram. Upper right quarter be WBC/BASO with, left portion be juvenile cell (IMI) use, the right sideEach scatter diagram that middle part is used for RET. Lower left quarter is RBC histogram, and right lower quadrant is PLTUse histogram.
Figure 14 shows as the above-mentioned DIFF being prepared by the body fluid measurement result of measuring samplePicture 110 on data processing equipment 3 displays 302. Demonstration is arranged at the top of picture 110The mark this shop viewing area 111 of mark this shop, its side is provided with patient's attribute display district. Mark this shop is aobviousThe left side that shows district 111 shows " F " that expression is measured with humoral determination pattern. Accordingly canClearly to recognize, this analysis result is humoral determination result. Measurement result viewing area is by availableThe several pages of formations that label 112 is selected. In this example, selected " humoral determination (body fluid) "Label.
On measured value viewing area 113, the body fluid different from the measurement result of blood measuring patternWith measuring entry name WBC-BF (WBC number), RBC-BF (RBC number), MN(multi-nucleus cell number is (neutral thin for (monocyte number (lymphocyte+unicellular)), PMNBorn of the same parents+basicyte+acidophil)), MN% (the monocyte ratio in leucocyte),PMN% (the apocyte ratio in leucocyte) and measured value, unit be corresponding demonstration respectively.At humoral determination be also provided with chart viewing area 114 same with blood measuring. Distribution map viewing area is aobviousBe shown with two distribution Figure 115, it is DIFF scatter diagram that top disperses point diagram. Bottom is divided into RBCUse histogram.
Figure 15 for having selected " retrieval BF (Research in Figure 14 picture 110 in label 112(BF)) the illustration of label. This picture, except showing search argument viewing area 116, also showsThe project same with picture 110. In search argument viewing area 116, show and be present in as shown in figure 10Population in population " HF-BF# ", the region HF of region HF comprises region with being present inHF and region MF at the ratio " HF-BF% " of the population in interior region, be present in and comprise districtTerritory HF and region MF are in the population " TC-BF# " in interior region. Separately, " HF-BF% "It is HF-BF and the ratio of TC-BF.
Figure 16 is that the storage sample guide look being presented on data processing equipment 3 displays 302 showsPicture 140. The 130th, patient's attribute display district. Its top is provided with by label and selects to show mensurationThe measurement result viewing area of result. Measurement result viewing area leftmost column 131 is for showing mensurationThe checking work of result is not done or is done. V represents to verify. Its right row 132 are for showing surveyMould-fixed. " F " represents the measurement result of humoral determination pattern. Although if be at humoral determinationUnder pattern, need the blank high value sample detecting, but do not carry out blank detection, for by itShow, mark can reverse F.
Above with regard to the 26S Proteasome Structure and Function of cellanalyzer of the present invention, to pack in advance haemocyte intoAnalyzer is that example is illustrated, but also can realize this function by control system, shouldControl system packs traditional cellanalyzer into, allows traditional cellanalyzer bring into play thisBright function.
In structure described in present embodiment, under blood measuring pattern to leukocyte differential count and body fluidUnder mode determination to leukocyte differential count separately specimen amount when formation determination sample, reagent type andAmount of reagent is all the same. Can be not limited to this, also can allow prepare under humoral determination pattern and classify in vain carefullyBorn of the same parents classify in vain more than preparing under blood measuring pattern respectively by specimen amount and the amount of reagent of measuring sampleSpecimen amount and the amount of reagent of measuring sample for cell. Owing under humoral determination pattern, leucocyte being dividedClass minute is longer than blood measuring pattern, measures required mensuration sample size also many, therefore,Do like this under the leukocyte differential count under blood measuring pattern and humoral determination pattern respectivelyLeukocyte differential count is prepared appropriate mensuration sample.
In the present embodiment, just with scattered light and fluorescence under humoral determination pattern to leucocyteThe structure of classification is set forth, but is not limited to this, also can use such as scattered light and absorb lightUnder humoral determination pattern to leukocyte differential count. Light absorbing mensuration can be by stain leukocytesColoring agent is sneaked into sample together with other reagent, and formation determination sample provides this mensuration sampleGive flow cell, make it to form sample stream in flow cell, this sample stream of illumination, by photoelectricity twoThe light collecting elements such as utmost point pipe receive the light that sample stream is sent. When leucocyte passes through in flow cell, light quiltLeucocyte absorbs, and the light harvesting amount that its degree of absorption can be used as light collecting element is caught. AboutThis light absorbing mensuration, No. 5122453rd, United States Patent (USP) and United States Patent (USP) the 5138181stOn number communique, deliver. Also can measure resistance and replace scattered light, by resistance value and absorption lightLeucocyte is classified.
Claims (10)
1. a haemocyte with humoral determination pattern and blood measuring pattern mensuration blood dividesAnalyse instrument, comprising:
Mode determination setup unit, for setting described humoral determination pattern or described blood measuringPattern;
Measure and start indicating member, for accepting to start the instruction of mensuration;
Optical information acquiring unit, described optical information acquiring unit has in order to use streaming thinBorn of the same parents' art obtain measure the celliferous optical information of institute in sample and with the light source of illumination mensuration sample,From the contained cell of this mensuration sample, obtain the forward scattering light light harvesting of its forward scattering optical informationDevice, from the contained cell of this mensuration sample, obtain the side scattered light collection of its lateral scattering optical informationLight device and obtain the side direction fluorescence of its side direction fluorescence information from the contained cell of this mensuration sampleOptical collector;
Analytic unit, described mode determination setup unit is set after described humoral determination pattern, whenDescribed mensuration starts indicating member while receiving the instruction that starts to measure, according to by body fluid and leucocyteIn the described mensuration sample that mensuration is prepared with reagent, obtained by described optical information acquiring unitDescribed lateral scattering optical information and described side direction fluorescence information obtain comprise fluorescence intensity weak theThe second area that one region, fluorescence intensity are strong and fluorescence intensity be positioned at described first area and described inThe scatter diagram in the 3rd region between second area, and to being distributed in the monokaryon in described the 3rd regionLeucocyte and multinuclear leucocyte and be distributed in the having except leucocyte of described second areaNucleus is counted;
Wherein, described body fluid refers to blood body fluid in addition.
2. cellanalyzer according to claim 1, is characterized in that: described analysisUnit calculate multinuclear leucocyte in leucocyte shared ratio or monocyte at leucocyteIn shared ratio.
3. cellanalyzer according to claim 1 and 2, is characterized in that: described inThe number of nucleated cells of analytic unit beyond leukocyte count and leucocyte, obtain full karyocyteNumber, then obtain leucocyte karyocyte in addition and the ratio of full karyocyte.
4. cellanalyzer according to claim 1, is characterized in that: described analysisThe contained cell of described mensuration sample is further separated erythrocyte ghost by unit.
5. cellanalyzer according to claim 1, is characterized in that: when not settingWhen described humoral determination pattern, measure beginning indicating member and accept to start after the instruction of mensuration, pointAnalyse unit according to measuring from blood and leucocyte the side of obtaining the mensuration sample of preparing with reagentBe number to scattered light information and side direction fluorescence information by the contained leukocyte differential count of described mensuration sampleIndividual subclass, and counting.
6. cellanalyzer according to claim 1, is characterized in that: also possess defeatedGo out unit, for the display frame of the analysis result of analytic unit described in output display.
7. cellanalyzer according to claim 1, is characterized in that: described body fluidBe selected from the dislysate that comprises cerebrospinal fluid, hydrothorax, ascites, pericardium liquid, joint fluid, peritoneal dialysisWith abdominal cavity cleaning liquid inside at interior liquid.
8. cellanalyzer according to claim 1, is characterized in that: described in have coreCell selects free macrophage, middle chrotoplast, tumour cell, erythrocyte ghost and combination structure thereofThe colony becoming.
9. a cellanalyzer, comprising:
Mode determination setup unit, for setting humoral determination pattern;
Measure and start indicating member, for accepting to start the instruction of mensuration;
Aspirating specimen unit, receives the described finger that starts mensuration when described mensuration starts indicating memberAfter showing, move sample for inhaling;
Measure sample and prepare unit, inhale with described aspirating specimen unit the sample and the leucocyte that move and surveySurely use reagent formation determination sample;
Optical information acquiring unit, described optical information acquiring unit has in order to use streaming thinBorn of the same parents' art obtain measure the celliferous optical information of institute in sample and with illumination by described mensuration samplePrepare the light source of the mensuration sample of preparing unit, from the contained cell of this mensuration sample, obtain before itTo the forward scattering light optical collector of scattered light information, obtain it from the contained cell of this mensuration sampleThe side scattered light optical collector of lateral scattering optical information and from the contained cell of this mensuration sampleObtain the side direction fluorescence optical collector of its side direction fluorescence information;
Analytic unit, the described side scattered light letter obtaining according to described optical information acquiring unitBreath and described side direction fluorescence information, classify to the contained cell of described mensuration sample, and to dividingThe described cell count of class;
After described humoral determination pattern is set by described mode determination setup unit, in described mensurationWhen beginning indicating member is received the instruction that starts mensuration, described mensuration sample is prepared described in unit useAspirating specimen unit is inhaled the body fluid and the described leucocyte mensuration reagent that move and is prepared described mensuration examinationSample, described analytic unit according to the described lateral scattering optical information obtaining from described mensuration sample andDescribed side direction fluorescence information obtain comprise fluorescence intensity weak first area, fluorescence intensity strong theTwo regions and fluorescence intensity be 3rd district between described first area and described second areaThe scatter diagram in territory, and to be distributed in the monocyte in described the 3rd region and multinuclear leucocyte withAnd the karyocyte except leucocyte that is distributed in described second area is counted;
Wherein, described body fluid refers to blood body fluid in addition.
10. cellanalyzer according to claim 9, is characterized in that: when not establishingWhen fixed described humoral determination pattern, measure beginning indicating member and accept to start after the instruction of mensuration,Described mensuration sample is prepared described aspirating specimen unit, unit and is inhaled the blood that moves and described white thinBorn of the same parents measure with reagent formation determination sample, and described analytic unit is according to obtaining from described mensuration sampleThe described lateral scattering optical information of getting and described side direction fluorescence information are by contained described mensuration sampleLeukocyte differential count is several subclass, and counting.
Priority Applications (4)
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| CN201610209662.9A CN105807038B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610208992.6A CN105807037B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610209661.4A CN105866012B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610212407.XA CN105891090B (en) | 2007-02-01 | 2008-01-31 | Blood cell analyzer, body fluid analysis method, and control system therefor |
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| JP2007022524 | 2007-02-01 | ||
| JP2007-022524 | 2007-02-01 | ||
| JP2007022524 | 2007-02-01 | ||
| JP2007119012A JP4926812B2 (en) | 2007-02-01 | 2007-04-27 | Blood cell analyzer and body fluid analysis method |
| JP2007-119012 | 2007-04-27 | ||
| JP2007119012 | 2007-04-27 |
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| CN201610208992.6A Division CN105807037B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610209661.4A Division CN105866012B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610212407.XA Division CN105891090B (en) | 2007-02-01 | 2008-01-31 | Blood cell analyzer, body fluid analysis method, and control system therefor |
| CN201610209662.9A Division CN105807038B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
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| CN201610208992.6A Active CN105807037B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN200810005239.2A Active CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
| CN201610212407.XA Active CN105891090B (en) | 2007-02-01 | 2008-01-31 | Blood cell analyzer, body fluid analysis method, and control system therefor |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN105807038B (en) | 2019-06-18 |
| CN101236195A (en) | 2008-08-06 |
| CN105807038A (en) | 2016-07-27 |
| CN105891090A (en) | 2016-08-24 |
| JP2008209386A (en) | 2008-09-11 |
| CN105807037A (en) | 2016-07-27 |
| JP4926812B2 (en) | 2012-05-09 |
| CN105807037B (en) | 2019-05-28 |
| CN105891090B (en) | 2020-01-17 |
| CN105866012A (en) | 2016-08-17 |
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