CN105807038B - Cellanalyzer, method for analyzing body fluid and its control system - Google Patents
Cellanalyzer, method for analyzing body fluid and its control system Download PDFInfo
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- CN105807038B CN105807038B CN201610209662.9A CN201610209662A CN105807038B CN 105807038 B CN105807038 B CN 105807038B CN 201610209662 A CN201610209662 A CN 201610209662A CN 105807038 B CN105807038 B CN 105807038B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/12—Coulter-counters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G01N2015/012—
-
- G01N2015/014—
-
- G01N2015/016—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1486—Counting the particles
Abstract
The present invention provides a kind of cellanalyzer, comprising: fluid device has and aspirate the aspirating specimen mouth of sample, and mixes and aspirate the sample and the reagent that mouth aspirates by described and prepare measurement sample；Detection part is capable of detecting when scattering light and fluorescence that the cell from the measurement sample for flowing through sheath flow pool obtains；Controller handles the measurement result exported from the detection part and obtains analysis result；And display；Wherein, the controller makes the display show the input picture for receiving the selection to blood measuring mode and humoral determination mode；If having selected the blood measuring mode, sample is put into mode at least and can specify following mode: operator aspirates the 1st mode of sample manually and transports and aspirate automatically the 2nd mode of sample；If having selected the humoral determination mode, the 1st mode can only be specified as sample and be put into mode.
Description
It is on January 31st, 2008 that the application, which is application No. is the 200810005239.2, applying date, entitled " haemocyte point
The divisional application for the Chinese invention patent application of analyzer, method for analyzing body fluid and its control system " submitted by same applicant.
Technical field:
The present invention relates to a kind of conduct samples can not only measure blood, can also measure cerebrospinal fluid (marrow liquid), hydrothorax (pleura
Liquid) and the blood such as ascites other than other body fluid cellanalyzer, method for analyzing body fluid and its control system.
Background technique:
In clinical examination field, common cellanalyzer is analyzed with picking up from the blood of body as tested sample, and
One of the reference that the analysis result is monitored as diagnosis and treatment.
Meanwhile clinical examination field is thirsted for easily measuring the body fluid other than the blood such as cerebrospinal fluid.Usual body fluid
In be practically free of cell, but when illness or related organ have tumour and damage, it finds that bleeding (haemocyte) and it is abnormal carefully
The cells such as born of the same parents, bacterium.
It is described on No. 2003/0215890 bulletin of U.S. Patent Application Publication No. and measures body about with cellanalyzer
The technology of cell in liquid.It is described on No. 2003/0215890 bulletin of U.S. Patent Application Publication No. accordingly, aldehyde, surface will be contained
The reagent components of activating agent and cyclodextrin are mixed with cerebrospinal fluid (CSF), preparation measurement sample, with ADVIA120 cytoanalyze point
Made measurement sample is analysed, classified according to cytological map shown in Figure 11 A ~ Figure 11 G of this bulletin and counts the cell in cerebrospinal fluid.
However, according to disclosed technology on No. 2003/0215890 bulletin of U.S. Patent Application Publication No., effectively as
Body fluid only analyzes cerebrospinal fluid (CSF), is not analyzed about body fluid such as such as ascites and hydrothorax.It is free of mostly in usual cerebrospinal fluid
Particle composition other than haemocyte, and in the body fluid other than the cerebrospinal fluid such as ascites and hydrothorax, contains sometimes because of patient disease
Chrotoplast, macrophage and tumour cell etc..In this way, when on No. 2003/0215890 bulletin of U.S. Patent Application Publication No.
When body fluid of the disclosed technology analysis containing the particle composition other than haemocyte, it is possible to cause such as in cytological map certain is thin
The problem of there is the particle composition other than haemocyte in born of the same parents region, can not obtain Correct Analysis result.
Summary of the invention:
The scope of the present invention is only by appended claims defined, not by this section summary of the invention in any degree
Statement limited.
The cellanalyzer for the measurement haemocyte that first side of the present invention provides includes: mode determination setup unit, is used
In setting humoral determination mode；Instruction to start the measurement unit, the instruction for receiving to start to measure；Optical information acquiring unit,
Illumination measures sample, obtains optical information from cell contained by the measurement sample；Analytical unit is set in above-mentioned humoral determination mode
After fixed, when said determination, which starts indicating unit, is connected to the instruction for starting measurement, used according to by humoral specimen and leucocyte measurement
Cell contained by the measurement sample is at least classified as white thin by the above-mentioned optical information obtained in the said determination sample of reagent preparation
Karyocyte other than born of the same parents and leucocyte, to the Other nucleated cells differential count other than leucocyte and leucocyte.
Leucocyte is divided into multinuclear leucocyte and monocyte by the analytical unit, respectively to multinuclear leucocyte and monokaryon
White blood cell count(WBC).
The analytical unit calculate multinuclear leucocyte in leucocyte shared ratio or monocyte in leucocyte
Shared ratio.
The analytical unit finds out full number of nucleated cells from the number of nucleated cells other than leukocyte count and leucocyte, then asks
The ratio between karyocyte other than leucocyte and full karyocyte out.
Cell contained by the measurement sample is further separated erythrocyte ghost by the analytical unit.
When not setting the humoral determination mode, after instruction to start the measurement unit receives the instruction for starting to measure, analysis
Unit will be described according to the optical information obtained from the measurement sample that blood preparation and leucocyte measurement are prepared with reagent
Measuring leukocyte differential count contained by sample is several subclass, and is counted.
It is also equipped with output unit, for exporting the display picture for showing the analysis result of the analytical unit.
The optical information is selected from the light for scattering optical information, the fluorescence information that cell issues, cell extinction that cell issues
Absorb information and the combination of these information.
The humoral specimen be selected from including cerebrospinal fluid, hydrothorax, ascites, pericardium liquid, joint fluid, peritoneal dialysis dialyzate and
Liquid including abdominal cavity cleaning liquid inside.
The karyocyte is selected from being made of macrophage, middle chrotoplast, tumour cell, erythrocyte ghost and combinations thereof
Group.
The cellanalyzer that second side of the present invention provides includes: mode determination setup unit, is surveyed for setting body fluid
Mould-fixed；Instruction to start the measurement unit, the instruction for receiving to start to measure；Aspirating specimen unit, for aspirating sample；Measurement
Sample preparation unit, the sample aspirated with aspirating specimen unit and leucocyte measurement reagent preparation measurement sample；Optical information
Acquiring unit, illumination measure sample, obtain optical information from cell contained by the measurement sample；Analytical unit, according to acquisition
Above-mentioned optical information classifies to cell contained by said determination sample, and the above-mentioned cell count to classification.Above-mentioned body fluid is surveyed
After mould-fixed is set by said determination mode setting unit, the instruction for starting to measure is connected to when said determination starts indicating unit
When, the humoral specimen and the measurement of above-mentioned leucocyte that said determination sample preparation unit is just aspirated by above-mentioned aspirating specimen unit are used and are tried
Agent prepares said determination sample, and above-mentioned analytical unit tries measurement according to the above-mentioned optical information obtained from said determination sample
Cell classification contained in sample is the cell other than leucocyte and leucocyte, and to the karyocyte other than leucocyte and leucocyte
It counts.
It is described after instruction to start the measurement unit receives the instruction for starting to measure when not setting the humoral determination mode
The blood preparation and leucocyte measurement reagent preparation that measurement sample preparation unit is aspirated with the aspirating specimen unit are surveyed
Determine sample, the analytical unit will be white contained by the measurement sample according to the optical information obtained from the measurement sample
Cell classification is several subclass, and is counted.
The method for analyzing body fluid that third side of the present invention provides includes: (a) step: setting mode determination, to set body
Liquid mode determination；(b) step: after the setting of humoral determination mode, receive the instruction for starting measurement；(c) step: receive above-mentioned beginning
After the instruction of measurement, the measurement sample that irradiation is prepared by humoral specimen and leucocyte measurement reagent is used up, from the measurement sample
Optical information is obtained in contained cell；(d) step: according to the above-mentioned optical information of acquisition, by cell contained by said determination sample
It is at least classified as karyocyte other than leucocyte and leucocyte, and to Other nucleated cells differential count other than leucocyte and leucocyte.
The method for analyzing body fluid further includes step e: after receiving the instruction for starting measurement, aspirating the body fluid mark
This, and the humoral specimen by aspirating and the leucocyte measurement reagent prepare the measurement sample.
(d) step includes by leukocyte differential count be multinuclear leucocyte and monocyte and to multinuclear leucocyte and
The step of monocyte counts respectively.
(d) step includes the step of monocyte ratio in multinuclear leucocyte ratio or the leucocyte asked in leucocyte
Suddenly.
4th side of the invention provides a kind of control system of body fluid analysis instrument, comprising: a kind of mode determination setting control
System sets mode determination, to set humoral determination mode；A kind of measurement instruction receives control system, humoral determination mode
After setting, receive the instruction for starting measurement；A kind of optical information acquisition control system, after receiving the above-mentioned instruction for starting measurement,
The measurement sample that irradiation is prepared by humoral specimen and leucocyte measurement reagent is used up, is obtained from cell contained by the measurement sample
Optical information；And a kind of cell classification and counting control system, according to the above-mentioned optical information of acquisition, by said determination sample institute
The karyocyte being at least classified as containing cell other than leucocyte and leucocyte, and to the karyocyte other than leucocyte and leucocyte
It counts.
Leukocyte differential count can be multinuclear leucocyte and monocyte and right by the cell classification and counting control system
Multinuclear leucocyte and monocyte count respectively.
The cell classification and counting control system can calculate single in multinuclear leucocyte ratio or leucocyte in leucocyte
Nuclear leukocyte ratio.
The cell classification and counting control system can further separate red blood cell blood from cell contained by the measurement sample
Shadow.
Detailed description of the invention:
Fig. 1 is the outside drawing of the cellanalyzer of an embodiment of the present invention.
Fig. 2 is the block diagram of analysis-e/or determining device.
Fig. 3 is the block diagram of fluid device.
Fig. 4 is that the optical system of white blood cell detection device shows figure.
Fig. 5 is the display figure of RBC/PLT detector.
Fig. 6 is the display figure of HGB detector.
Fig. 7 is the flow chart of sample measurement processing.
Fig. 8 is the attached drawing for setting the display picture of mode determination.
Fig. 9 is the flow chart of preamble column processing.
Figure 10 is the ideograph for the scatter plot that measurement is measured sample by DIFF prepared by body fluid.
Figure 11 is the comparison diagram of the cellanalyzer measurement result of embodiment and the measurement result of counter point.
Figure 12 is the ideograph for the scatter plot that measurement is measured sample by DIFF prepared by blood.
Figure 13 is the display picture of the measurement result of blood measuring mode.
Figure 14 is the display picture of the measurement result of humoral determination mode.
Figure 15 is the display picture of the measurement result of humoral determination mode.
Figure 16 is the display picture of the measurement result of humoral determination mode.
Figure 17 is the confirmation screen of beginning blank detection shown under humoral determination mode.
Specific embodiment:
Below according to Detailed description of the invention a specific embodiment of the invention.
Fig. 1 show cellanalyzer 1.This cellanalyzer 1 is as the multinomial full-automatic blood for being used for blood test
Cytoanalyze, can the blood preparation inner to specimen container (heparin tube) be measured, obtain indicate sample in contained blood it is thin
The characteristic information of born of the same parents' feature, and this feature information is analyzed and processed.This cellanalyzer 1 can be with analysing body fluid.?
The cellanalyzer of present embodiment, analysis object body fluid, which refers to, is present in endoceliac coelomic fluid other than blood.Specifically
It says and refers to cerebrospinal fluid (marrow liquid, CSF: the hydrops of the ventricles of the brain and subarachnoid space), hydrothorax (liquor pleurae, PE: pleural effusion), ascites (abdomen
Chamber hydrops), pericardium liquid (chambers of the heart hydrops), joint fluid (synovia: the liquid in joint, bursa synovialis and stndon sheath) etc..Peritoneal dialysis
(CAPD) dialyzate and abdominal cavity cleaning liquid inside etc. also can be used as a kind of of body fluid and analyzed.Almost do not have in these usual liquid
There is cell, but when illness or related organ have tumour and be damaged, it is possible to thin containing haemocyte, abnormal cell and bacterium etc.
Born of the same parents.Such as cerebrospinal fluid, following clinical deduction can be made from analysis result.For example, may be spider web if red blood cell increases
Film bleed bottom can suspect the infectious disease of trouble suspected of meningitis, if acidophic cell increases if neutrophil cell increases
Sick (helminth and fungi) can be suspected if monocyte increases for Tuberculous meningitis and viral meningitis, if other
Cell increases, then can suspect and shift for tumour to meninges.As for ascites and hydrothorax etc., if also thin containing middle skin in addition to haemocyte
The karyocytes such as born of the same parents, macrophage and tumour cell, so that it may thin by analyzing this blood as the index for suspecting the diseases such as cancer
Karyocyte other than born of the same parents can get these indexs.
Cellanalyzer 1 is by that can measure as the blood of sample and the measurement device 2 of body fluid and export to measurement device 2
Measurement result handled, obtain analysis result data processing equipment 3 constitute.Data processing equipment 3 includes controller
301, display 302 and input equipment 303.In Fig. 1, measurement device 2 is respectively deposited as a device with data processing equipment 3
It can also be combined into one as a device.
Fig. 2 is the block diagram of the measurement device 2 of cellanalyzer 1.As shown in Fig. 2, measurement device 2 is detected including haemocyte
Part 4, analogue signal processor 5, the microcomputer 6, display behaviour that the output signal (analog signal) of detection part 4 is handled
Make part 7 and measures the device for mechanical part 8 of blood and body fluid.Device for mechanical part 8 includes following fluid device 81.
Fig. 3 is the structural block diagram of fluid device 81.As shown in figure 3, fluid device 81 includes aspirating specimen mouth 18, Shuo Geshi
Agent container 11, sampling valve 12 and reaction warehouse 13 ~ 17.Aspirating specimen mouth 18 aspirates sample from specimen container, which is sent to and is adopted
Sample valve 12.The sample of importing is divided into a certain amount of several equal parts by sampling valve 12.This segmentation number is because of mode determination (each measurement mould
Formula) and it is different, and the CBC type specimen for measuring red blood cell number, leukocyte count, platelet count and hemoglobin concentration is divided into three
Equal part.Add the CBC+DIFF mode of the classification of leucocyte five again on above-mentioned CBC mode determination, then sample quadrisection.?
The CBC+DIFF+RET mode for adding measurement granulophilocyte in CBC+DIFF measurement item again, is divided into five equal parts.Equally, CBC+
It added with the CBC+DIFF+NRBC mode of nucleated red blood cell measurement item is also again that sample is divided into five etc. in the measurement item of DIFF mode
Point.The CBC+DIFF+RET+NRBC mode for adding measurement erythroblast again in CBC+DIFF mode+RET measurement item, then divide
For six equal parts.The above mode determination is the blood measuring mode for measuring blood entirely.Finally, in the humoral determination mode of measurement body fluid
In, sample is divided into bisection.
Reagent (dilution) imports this sampling valve 12 from reagent container 11, and the sample equal part divided conveys together with reagent
To reaction warehouse 13 ~ 17 and aftermentioned HGB detector 43.Reaction warehouse 13 is extracted certain by not shown constant displacement pump offer sampling valve 12
Sample (equal part), a certain amount of dilution and a certain amount of dyeing liquor of amount, these samples and reagent are mixed, and leucocyte is prepared
The measurement sample of four classification (DIFF).
As dilution, reagent " the leucocyte hemolysin of SYSMEX Co., Ltd's offer can be suitably used
STROMATOLYSER-4DL".This reagent contains surfactant, can be with lysed erythrocyte.Dyeing liquor equally can be suitably used uncommon
The reagent " classification of leucocyte four liquid STROMATOLYSER-4DS " that gloomy Meikang Co., Ltd. provides.This dyeing liquor contain ethylene glycol,
Low alcohol, polymethine, after above-mentioned dilution haemolysis, haemocyte composition is colored, and finally produces 50 times of dilution samples.
If selecting body fluid mode determination, to classify with the same specimen amount of measurement sample, together with this leucocyte four
The condition of sample reagent and same reagent amount prepares leukocyte differential count measurement sample.But as described later, humoral determination mode
Leukocyte differential count leucocyte not instead of four classes, two classes.
Reaction warehouse 14 is provided a certain amount of sample, a certain amount of dilution hemolytic agent that sampling valve 12 acquires by not shown constant displacement pump
With a certain amount of dyeing liquor, these samples and reagent are mixed, production erythroblast (NRBC) measurement measurement sample.
Reaction warehouse 15 is provided a certain amount of sample, a certain amount of dilution hemolytic agent that sampling valve 12 acquires by not shown constant displacement pump
With a certain amount of dyeing liquor, these samples and reagent are mixed, production granulophilocyte (RET) measurement measurement sample.
Reaction warehouse 16 is provided a certain amount of sample and a certain amount of dilution haemolysis that sampling valve 12 acquires by not shown constant displacement pump
Agent mixes these samples and reagent, makes leucocyte and basicyte (WBC/BASO) measurement measurement sample.
Reaction warehouse 17 is provided a certain amount of sample, a certain amount of dilution that sampling valve 12 acquires by not shown constant displacement pump, will
These samples and reagent mixing, production red blood cell/blood platelet (RET/PLT) measurement measurement sample.
In addition, a certain amount of sample, a certain amount of dilution hemolytic agent that sampling valve 12 acquires also supply aftermentioned HGB detector 43.
Detection part 4 has the white blood cell detection device 41 of detection leucocyte.It is red thin that this white blood cell detection device 41 has been also used for core
The detection of born of the same parents and granulophilocyte.For detection part 4 in addition to white blood cell detection device 41, there are also measure red blood cell number and platelet count
The HGB detector 43 of hemochrome amount in RBC/PLT detector 42 and measurement blood.
Above-mentioned white blood cell detection device 41 is mainly made of fluorescence detector, specifically, by the inspection with flow cytometry
Device is surveyed to constitute.Here, so-called cell art is the physical property and chemical property for measuring cell and other biological particle, so-called stream
Formula cell art refers to: the method for allowing these particles to pass through from thread, be measured.Fig. 4 show the light of white blood cell detection device 41
System.In the figure, the light beam projected from laser luminescence diode 401 is irradiated to by collimating mirror 402 flows through sheath flow pool 403
Interior haemocyte.The forward direction that haemocyte in this white blood cell detection device 41 detection sheath flow pool 403 is issued under laser irradiation dissipates
Luminous intensity, lateral scattering luminous intensity and lateral fluorescence intensity are penetrated, in this, as haemocyte characteristic parameter.
Here, the scattering of light is to become barrier on the direction of travel of light due to this particle of haemocyte, light is therefore
Change phenomenon caused by its direction of travel.The particle characteristics in relation to particle size and composition can be obtained by detecting this scattering light
Information.Forward scattering light refers to the scattering light essentially identical with the direction of travel of institute irradiation light that particle issues.It is dissipated from forward direction
The characteristic information in relation to particle (haemocyte) size can be obtained by penetrating light.Side scattered light be particle issue with institute's irradiation light
Scattering light slightly in vertical direction.The characteristic information in relation to inside particles can be obtained from side scattered light.When laser irradiation arrives
When on haemocyte particle, lateral scattering luminous intensity depends on complexity (shape, size, density and the particle of core of cell interior
Quantity).Therefore, it can be classified (identification) haemocyte using this characteristic of lateral scattering luminous intensity, and measure blood cell count
Amount.In addition, already described present embodiment takes the structure for using forward scattering light and side scattered light as scattering light, but unlimited
In this, as long as the scattered light signal of reflection particle characteristics required for analysis can be obtained, scattering light penetrates sheath for light source
The angle for flowing the optical axis of the light of pond irradiation is unlimited.
Illumination such as that fluorescent material of the haemocyte through dyeing, then issue light longer than shone optical wavelength.Fluorescence
Intensity is to dye more better stronger, measures this fluorescence intensity and is achieved with the characteristic information in relation to blood cell staining degree.Therefore, root
According to the difference of (lateral) fluorescence intensity, the measurement such as classify can be carried out to leucocyte.
As shown in figure 4, the forward scattering light that the haemocyte (leucocyte and erythroblast) for flowing through sheath flow pool 403 issues is logical
Condenser 404 and pin hole 405 is crossed to be received by light emitting diode (forward scattering light optical collector) 406.Side scattered light passes through optically focused
Mirror 407, dichronic mirror 408, optical film 409 and pin hole 410 are received by photomultiplier tube (side scattered light optical collector) 411.Laterally
Fluorescence is received by condenser 407 and dichronic mirror 408 by photomultiplier tube (lateral fluorescence optical collector) 412.From each optical collector
406, the suffered optical signals of 411 and 412 outputs respectively by the analogue signal processor 5 that is made of amplifier 51,52,53 etc. into
After the analog signal processings such as row amplification and waveform processing, it is transported to microcomputer 6.
In the following, being illustrated with regard to the structure of RBC/PLT detector 42.Fig. 5 is the brief configuration mould of RBC/PLT detector 42
Formula figure.RBC/PLT detector 42 can be with sheath stream DC detection method measurement red blood cell number and platelet count.RBC/PLT detector 42
There is sheath flow pool 42a as shown in Figure 5.This sheath flow pool 42a is equipped with the adding mouth 42b of upward opening, and sample can add from reaction warehouse 17
Enter this adding mouth 42b.There are also the cone cell sample storehouse 42c being tapered upwards, above-mentioned adding mouth 42b just to configure herein by sheath flow pool 42a
The center of inside of sample storehouse 42c.The sample storehouse upper end 42c is equipped with hole 42d, this hole 42d is just opposite with the center adding mouth 42b.For sample device
The measurement sample of offer transports upwards from the front end of adding mouth 42b, and at the same time, preceding sheath fluid is supplied to sample storehouse 42c, preceding sheath fluid to
It is upper to flow to hole 42d.Here, measurement sample flows under the encirclement of preceding sheath fluid, cone cell sample storehouse 42c makes to measure sample rheology
Carefully, the haemocyte through hole 42d one by one in sample is measured.Hole 42d sets electrode, is provided with DC current between this electrode.Detection is when survey
This electric signal is output to controller 25 by the variation for determining the D.C. resistance of hole 42d when sample flows through hole 42d.Above-mentioned D.C. resistance
It will increase in haemocyte through hole 42d, therefore, this electric signal reflects the information of haemocyte through hole 42d, by this
Electric signal carries out signal processing, to red blood cell and platelet count.
The top of hole 42d is equipped with the lower recovery tube 42e extended upwards.This recovery tube 42e is configured at through hole 42d and sample storehouse
In 42c connected sample storehouse 42f.The lower end recovery tube 42e is separated with sample storehouse 42f inner wall.Sample storehouse 42f has rear sheath fluid to provide, hereafter sheath
Liquid flows downward along the lateral area of the recovery tube 42e of sample storehouse 42f.The rear sheath fluid flowed through on the outside of recovery tube 42e reaches sample storehouse
Behind the lower end 42f, by flowing to inside recovery tube 42e between the lower end recovery tube 42e and the inner wall of sample storehouse 42f.Therefore it can prevent
The haemocyte of through hole 42d flows back, to prevent the erroneous detection of haemocyte.
The structure of HGB detector 43 is illustrated below.HGB detector 43 can measure blood by SLS hemoglobin method
Amount of pigment (HGB).Fig. 6 is the oblique view of 43 structure of HGB detector.HGB detector 43 have dress dilution sample sample pond 43a, to
Light collecting element 43c of the sample pond 43a luminous light emitting diode 43b and reception through the transmitted light of sample pond 43a.Sampling valve 12 is quantitative
Blood be diluted liquid and certain haemolysis dilution agent by certain dilution rate, preparation dilution sample.This hemolytic agent has will be in blood
Hemoglobin be converted to the property of SLS- hemoglobin.This dilution sample feeds to sample pond 43a, deposits in sample pond 43a.?
Under this state, light emitting diode 43b is made to shine, transmitted light is first by the light harvesting being oppositely disposed every sample pond 43a and light emitting diode 43b
Part 43c is received.The sent out wavelength light of light emitting diode 43b is easy to by SLS- hemoglobin absorption, and sample pond 43a is by translucency height
Plastic material be made, therefore, it is that light emitting diode 43b transmitting light is only diluted after sample absorbs that light collecting element 43c is received
Transmitted light.Light collecting element 43c will transport to microcomputer 6 with collection light quantity (absorbance) corresponding electric signal, microcomputer 6 by this absorbance and
The dulling luminosity ratio of the only dilution of measured in advance is compared with calculating Hemoglobin Value.
Microcomputer 6 has the A/D converter 61 that the analog signal that analogue signal processor 5 provides is converted to digital signal.
The output valve of A/D converter 61 is transported to the exerciser 62 of microcomputer 6, calculates in exerciser 62, carries out one to collection optical signal
Fixed processing.Exerciser 62 according to the output valve of detection part 4 make distributed data (two-dimentional scatter plot (unfiled) and it is one-dimensional directly
Side's figure).
Microcomputer 6 includes by controlling the controller 63 constituted with processor operation with memory with processor and control and by dividing
The data analysis unit 64 that analysis processor and the memory of analysis processor operation are constituted.Controller 63 for control by
Automatic supply heparin tube for sample device (diagram is omitted), the instrument mechanical part 8 of the compositions such as fluid system of sample preparation and measurement
And other parts.Data analysis unit 64 is used to carry out the analyses such as screening to each distributed data to handle.Analysis result passes through interface
65 are transported to external data processing equipment 3, carry out the display processing such as data display and storing data.
Microcomputer 6 has the interface connecting with display operation part 7 66 and the interface connecting with device for mechanical part 8 67.It drills
It calculates device 62, controller 63 and interface 66,67 to connect by bus 68, controller 63 and data analysis unit 64 are connected by bus 69
It connects.Include on display operation part 7 operator assign start measurement instruction start switch and display instrument state, various
Setting value and the touch-screen type liquid crystal display analyzed result, receive operator's input.
The operation with regard to the cellanalyzer of present embodiment 1 is illustrated below.Fig. 7 is the sample point of present embodiment
The flow chart of analyzer operation.User (operator) connects the power supply (step S1) of cellanalyzer 1, starts blood cell analysis
Instrument 1.This cellanalyzer 1 first carries out self-test (step S2) when starting.In self-test, not only tests microcomputer 6, checks haemocyte
The operating condition of each operation mechanical part of analyzer 1 is also measured the blank detection of the blank sample without sample.So
Afterwards, microcomputer 6 is initially set (step S3) to mode determination.This initial set value is CBC+DIFF mode.Specifically,
In the processing of step S3, the parameter (service condition) of setting measurement blood, reaction warehouse such as used and minute setting.Such as
This, the sample analyzer of present embodiment is using blood measuring mode as initial launch mode.Accordingly, cellanalyzer 1 is in
The acceptable standby mode for starting measurement.Microcomputer 6 shows the picture (step S4) of notice standby mode on a liquid crystal display.
Under this standby mode, operator can switch mode determination by operation display operation part 7.Fig. 8 is to set
Determine the input image mode figure of mode determination.There are sample number 120, sample to be put into schema category 121, subitem detection in this picture
(mode determination) type 122 and each display picture of species of samples 123.Sample is put into mode equipped with Three models: operator
Specimen container insertion aspirating specimen mouth 18 is carried out to the manual mode of aspirating specimen manually；Operator is in advance by sample and reagent
It is mixed with measurement sample, aspirates the Trace Blood pre-dilution mode of the measurement sample with aspirating specimen mouth 18；By transporting mark automatically
The conveyer of this container provides the closed mode of sample.As species of samples, be equipped with normal blood sample conventional (Normal),
HPC(hematopoietic progenitor cells) sample (HPC) and body fluid (Body Fluid).Operator can respectively specify that sample is put into mode, surveys
Mould-fixed and species of samples.If operator specifies blood measuring mode, species of samples is appointed as routine (Normal),
Any sample is specified to be put into mode and mode determination again.If specified humoral determination mode, operator are being put into mould respectively
" manual mode " is specified in formula, specifies " CBC+DIFF ", " CBC+DIFF+RET ", " CBC+DIFF+NRBC " in subitem detection
One of " CBC+DIFF+NRBC+RET " specifies body fluid (Body Fluid) in species of samples.In step s 4, it operates
The so specified desired mode determination of personnel.If operator does not change mode determination initially set and carries out blood survey
(N is selected in step S5) if fixed, then by starting switch and assign to start measurement instruction.The reception of microcomputer 6 starts measurement instruction (step
S6), blood preparation (step S7) is aspirated from aspirating specimen mouth.
After blood preparation aspirates, sample as described above is imported into sampling valve 12, detects type according to the subitem of mode determination
Sample (step S14) required for modulation measures.Then the measurement for implementing measurement sample runs (step S16).For example, subitem inspection
When survey type is set as " 7 ", the various measurement samples of HGB, WBC/BASO, DIFF, RET, NRBC, RBC/PLT are prepared.Then
WBC/BASO, DIFF, RET, NRBC are measured with measurement sample by white blood cell detection device 41, by RBC/PLT detector 42
RBC/PLT is measured with measurement sample, HGB is measured with measurement sample by HGB detector 43.At this point, leucocyte
Detector 41 is provided only with one, therefore, NRBC, WBC/BASO, DIFF, RET respectively measure sample by NRBC, WBC/BASO,
The sequence of DIFF, RET successively import white blood cell detection device 41, measure item by item.In this measurement operation, exerciser 62 draws grain
Sub- distribution map (scatter plot, histogram).Here, just being said according to the process that DIFF measures gained optical information drafting scatter plot
It is bright.Exerciser 62 is with side scattered light in the collection optical signal that is exported in DIFF is measured by white blood cell detection device 41 and lateral glimmering
Optical signal is characterized parameter, draws two-dimentional scatter plot (particle distribution figure).This scatter plot (hereinafter referred to as DIFF scatter plot) is with side
It is X-axis, draws by Y-axis of lateral fluorescence intensity to scattered light intensity, it is general " erythrocyte ghost population ", " lymph occur
Population ", " monokaryon population ", " neutrophilia+basophilla population " and " acidophil granules subgroup ".These populations are by data point
Analysis unit 64 is identified by processing DIFF scatter plot.
Then, (step S18) is analyzed and processed according to measurement gained particle distribution figure.In the processing of analysis herein, microcomputer 6
64 dialogue cell detector 41 of data analysis unit measurement DIFF measurement sample when the DIFF scatter plot point drawn of exerciser 62
Class are as follows: (lymphocyte populations, monocyte group, neutrophilia+basicyte group and acidophilia are thin by four leucocyte groups shown in Figure 12
Born of the same parents group) and erythrocyte ghost group.In the analysis processing of present embodiment, the weight of each particle and each group that are divided from scatter plot
Degree of membership of the available each particle in the distance between heart position to each group.It is divided into each group according to each particle of these degree of membership.
This method for classifying particles is documented on patent disclosure Heisei 5-149863 bulletin.Obtained by WBC/BASO measurement
On scatter plot, it is classified as leucocyte group and erythrocyte ghost group other than basicyte group, basicyte.It is dissipated further according to DIFF
The result (referring to Fig.1 2) and WBC/BASO Discrete point analysis that dot map analysis processing is classified to leucocyte four and counted handle dialogue
Cell two is classified and what is counted classifies as a result, carrying out five to leucocyte contained by blood preparation.Specifically, data analysis unit 64
WBC/BASO scatter plot point is subtracted from DIFF Discrete point analysis processing gained " neutrophil(e) cell+basicyte blood cell count "
Analysis processing gained " blood cell count of basicyte ", can obtain the blood cell count and basicyte of neutrophil(e) cell respectively
Blood cell count.Accordingly, leucocyte is by five classification (lymphocyte, monocyte, neutrophil(e) cell, basicyte and acidophilus
Property cell), obtain every blood cell count.In addition, detection is believed according to the feature that detector 42 measures in RBC/PLT measurement
The curve valley for ceasing the one dimensional histograms drawn classifies to red blood cell and blood platelet.The analysis result so obtained is output to number
On the display 302 of processing unit 3 (step S20).
On the other hand, if it is humoral determination mode that microcomputer 6 receives specified mode determination in step S5 as described above
When input, it is set for parameter (service condition) reaction warehouse such as used, the minute setting (step S8) of humoral determination.?
In present embodiment, three times when minute is blood measuring as described later.
(step when mode determination is switched to humoral determination mode by other mode determinations (being herein blood measuring mode)
S9), measurement device 2 starts preamble column processing (step S10).Series processing is prepared for humoral determination before this.Humoral determination
What is measured under mode is the low sample of haemocyte components and concentration, therefore, (is shown as " 1: often in fig. 8 from blood measuring mode
Rule ") switching will carry out preamble column processing when being set as humoral determination mode, to ensure that background does not interfere with humoral determination knot
Fruit.
Preamble column processing includes blank detection.Blank detection judgment criteria in this preamble column processing compares determination of blood cell
The judgment criteria of the detection of blank conducted in mode (for example carrying out after switch power supply and after automatic cleaning) is tightened up, setting
Value is one or less several points.In addition, when setting by humoral determination pattern switching as blood measuring mode, because background influence is (residual
Stay the influence of object) generally do not involve blood measuring as a result, so item preamble column processing without.In humoral determination mode repeatedly
When measuring humoral specimen, because not will receive the influence of background usually, without preamble column processing yet.But body fluid mark
Also some contains a large amount of particles for this, therefore, when humoral specimen analysis result exceeds certain value, will appear " measurement knot on interface
Fruit is too high, it is possible under the influence of the measurement of time sample, blank detection will be carried out.Please by " confirmation "." etc. information, notify operator
It is possible that influencing following analyzing specimen result.Operator presses " confirmation " button, can carry out blank detection.At this point, interface
Being equipped with " suspension " button can also detect without blank as long as operator presses " suspension " button, move to standby interface.
If not carrying out blank detection, symbol preferably low to measurement result Label reliability.Necessity can be only defined in by doing so
When additional blank detection, to prevent the waste of time and reagent class.
The presequence processing step that Fig. 9, which is mode determination, to be implemented when from blood measuring pattern switching being humoral determination mode
Flow chart.Cellanalyzer 1 implements blank detection (step S31), microcomputer 6 by measuring blank sample in measurement device 2
By measurement result compared with certain feasible value, judge whether measurement result is lower than feasible value (step S32).When measurement result is lower than
When feasible value, microcomputer 6 terminates presequence operation, recovery processing.When measured value is greater than feasible value, microcomputer 6 is judged whether to
Stipulated number (such as three times) blank detection (step S33) returns to step if blank detection number is not up to stipulated number
Rapid S31 is again carried out blank detection in above-mentioned stipulated number.If blank detection assay result is low not yet in stipulated number
In feasible value, then blank determination result is shown on display operation part 7 and is pressed including " confirmation " button, " blank detection "
Picture (step S34) including button, " automatic cleaning " button.If operator presses " confirmation " button (step S35), microcomputer
6 terminate presequence operation, recovery processing.If pressing " blank detection " button (step S36), microcomputer 6 returns process to step
Rapid S31 carries out blank detection again.If pressing " automatic cleaning " button (step S37), 6 implementation of microcomputer with special cleaning from
After dynamic cleaning (step S38), step S31 is returned process to, carries out blank detection again.
Above-mentioned presequence after treatment, cellanalyzer 1 return to standby mode (step S11).Operator starts
When humoral determination, as when the manual measurement of blood preparation, the aspirating specimen mouth 18 of measurement device 2 is inserted into specimen container
Humoral specimen, by starting to switch.Microcomputer 6, which is connected to, to be started to start to aspirate humoral specimen (step after measurement indicates (step S12)
S13).
After humoral specimen aspirates, humoral specimen is imported into sampling valve 91 as blood preparation.It is prepared by reaction warehouse 13
RBC/PLT measures sample (step S15).Then, DIFF is measured by white blood cell detection device 41 and measures sample, detected by RBC/PLT
Device 42 measures RBC/PLT measurement sample (step S17).In the state of humoral determination mode, measured in white blood cell detection device 41
Be only DIFF measure sample, therefore, even if minute is longer than the minute of blood measuring mode, it is also possible to compare blood
Measurement is completed when measurement in shorter time.In this way, by the minute of humoral determination is extended than blood measuring measurement
Time is long, and the analysis precision of the low humoral specimen of particle concentration can be improved.Minute is longer, and the population of counting will be got over
More, measurement accuracy will improve.But minute is too long, sample disposal ability can decline, while measurement sample being transported to
The ability of the syringe pump of white blood cell detection device 41 is limited, therefore is appropriate with 2 ~ 6 times.In the present embodiment, by humoral determination mould
Minute when formula is set as 3 times when blood measuring mode.
On the other hand, it is all to import resistive detectors 41 under any mode determination that RBC/PLT, which measures sample, certain
It is measured under the conditions of a fluid stream.Then it is analyzed and processed (step S19) according to measurement gained characteristic information, analysis result output
To the display 302(step S21 of data processing equipment 3).In the analysis processing under blood measuring mode, DIFF scatterplot is analyzed
Figure etc., calculate five kinds of leukocyte subsets (neutrophil cell: NEUT, lymphocyte: LYMPH, monocyte: MONO, acidophil:
EO, basocyte: BASO) information (quantity and ratio), but under humoral determination mode analysis processing in, because of blood sometimes
Cell number is seldom or by breakage, is that (monocyte: MN, multicore are thin for two kinds of subclass with the formal classification of part integration therefore
Born of the same parents: PMN).Lymphocyte and monocyte belong to monocyte, and neutrophil(e) cell, acidophic cell and basicyte belong to
Apocyte.This sorting algorithm is identical as in the algorithm illustrated in the analysis processing under blood measuring mode, therefore omits and say
It is bright.
Then, (step S22) is compared to the analysis result and feasible value (determined threshold values) that obtain in step S19.This
Feasible value used in blank detection in feasible value and the preamble column processing of step S10 is same value.When analysis result is greater than
("Yes" is selected in step S22) when feasible value, then the confirmation interface 151 of the detection of beginning blank shown in Figure 17 is shown in step S23.
Show on this confirmation interface 151: " measurement result is too high, it is possible to influence following sample measurement for display.It will carry out blank inspection
It surveys.Please by " confirmation "." information information display at 152, ACK button 153 and cancel button 154.Next, it is judged that user is defeated
That enter is ACK button 153 or cancel button 154(step S24), if input is that ACK button (selects in step s 24
" confirmation "), then implement blank detection (step S25).When the analysis result obtained in step S19 is less than feasible value (in step
S22 selects "No") or input be cancel button when (in step s 24 select " cancellations "), then without blank detection, return step
The processing of S5.
(macrophage and middle chrotoplast, tumour are thin for the abnormal particle for existing sometimes other than haemocyte in body fluid samples
Born of the same parents etc.).The case where there are these abnormal particles in cerebrospinal fluid is rarely found but relatively common in other body fluid hydrothorax and ascites.Cause
This will accurately classify to the haemocyte in body fluid regardless of body fluid type and be counted it is necessary to exclude these abnormal grains
The influence of son.Therefore, this is appeared on the upside of the DIFF scatter plot of this cellanalyzer the present invention is based on abnormal particle newly to recognize
Know, instrument is enable more accurately to measure the leucocyte in body fluid samples as target.This point is in traditional technology above-mentioned
In do not account for.
Figure 10 is that present embodiment cellanalyzer 1 is measured under humoral determination mode, analyzed by body fluid and leucocyte
The ideograph of DIFF measurement sample scatter plot obtained prepared by measurement reagent.The longitudinal axis of scatter plot indicates that lateral fluorescence is strong
It spends (more top, fluorescence intensity is stronger), horizontal axis indicates lateral scattering luminous intensity (more keeping right, scattered light intensity is stronger).Scatter plot
The weak region LF of fluorescence intensity the erythrocyte ghost Gc of haemolysis generation is distributed with, middle skin is distributed in the strong region HF of fluorescence intensity
Monocyte Mc, multinuclear leucocyte Pc is distributed in the exception particle such as cell, intermediate region MF.Therefore, in the analysis of scatter plot
In, the particle composition to be distributed in the region MF in addition to region LF and HF is analyzed as leucocyte, it is classified as above-mentioned two class,
And it counts.In addition, include lymphocyte and monocyte in monocyte Mc, in multinuclear leucocyte Pc comprising neutrophil cell,
Acidophic cell and basicyte.
When leucocyte in such analysing body fluid, also contained blood cell count is seldom or impaired in body fluid sometimes, therefore conduct
Leukocyte differential count is that monocyte and multinuclear leucocyte count by clinically significant information.
In addition, occasionally there are abnormal particle (macrophage and the middle chrotoplasts, tumour cell other than haemocyte in body fluid
Deng).The case where there are these abnormal particles in cerebrospinal fluid is rarely found but relatively common in other body fluid hydrothorax and ascites.Scheming
In 10 scatter plot, the karyocyte other than this leucocyte is distributed in region HF.It in the present embodiment, can be by leucocyte
Karyocyte and leukocyte differentiation in addition therefore can even if in body fluid containing the karyocyte other than this leucocyte
Find out correct leukocyte count.By counting to the cell for appearing in region HF, the journey of abnormal cell appearance can be provided
Degree.In the present embodiment, each cell is divided by region LF, MF and HF according to the threshold values for distinguishing each region, it can also be artificial
Change this threshold values.
Figure 11 is the appropriateness to show above-mentioned Discrete point analysis method, and compares the blood cell analysis using present embodiment
1 income analysis result of instrument and the attached drawing for using count results obtained by counter point.Tested sample is hydrothorax, and " this law " in figure indicates
The 1 calculated leukocyte count (WBC) of cellanalyzer of present embodiment and other abnormal populations (Others), " Ref "
Indicate counter point (cell count pond direct counting method (Fuchs-Rosenthal plate) and site spin method) calculated result.
Example 1,2,3 is all the hydrothorax that analysis has abnormal particle largely to occur results, it can be seen that the blood cell analysis of present embodiment
There is correlativity between 1 income analysis result of instrument and counter point.
Figure 13 is that the analysis of sample is measured as the above-mentioned DIFF prepared by blood as the result is shown in data processing equipment 3
Picture 100 on display 302.The sample viewing area of display sample number 101 is arranged at the top of picture 100, and side is equipped with aobvious
Show the attribute display area of patient attribute.Attribute display area specifically shows sample number, patient ID, patient's name, date of birth
Day, gender, ward, attending physician, measurement date, minute and remarks etc..Attribute display area lower part is equipped with display measurement
As a result measurement result viewing area.Measurement result viewing area is constituted by several pages, these pages can by select several labels 102 come
Show picture.Label has several for homepage, chart picture and sundry item.Figure 12 is display when chart label selects
Picture.The left-half of measurement result viewing area is equipped with measured value viewing area 103 and the display of the measured value of display measurement result
The chart viewing area 104 of chart, right half part are equipped with the distribution map viewing area 105 of the distribution map of display measurement result.Measured value
Viewing area show WBC, RBC ..., NEUT# ... BASO#, NEUT% ..., projects, data and the unit such as BASO%, chart viewing area
The mark that 104 displays are suspected about the sample exception that can be used as useful information in clinical examination and disease of WBC, PLT, RBC or RET
Remember result.
Distribution map viewing area 105 shows six distribution maps.The scatter plot of upper left quarter is DIFF scatter plot.Upper right quarter is
WBC/BASO is that juvenile cell (IMI) is used with, left portion, and right middle is each scatter plot of RET.Lower left quarter is RBC histogram
Figure, right lower quadrant are PLT histogram.
Figure 14 is to be shown in data processing equipment 3 with the measurement result of measurement sample as the above-mentioned DIFF prepared by body fluid
Picture 110 on display 302.The sample viewing area 111 of display sample number is arranged at the top of picture 110, and side, which is equipped with, suffers from
Person's attribute display area.The left side of sample viewing area 111 shows " F " for indicating to be measured with humoral determination mode.Accordingly
It can be expressly understood that, this analysis is the result is that humoral determination result.Selected by available label 112 several pages of measurement result viewing area
It constitutes.In this example, the label of " humoral determination (body fluid) " has been selected.
On measured value viewing area 113, the body fluid different from the measurement result of blood measuring mode measurement item name
WBC-BF(WBC number), RBC-BF(RBC number), MN#(monocyte number (lymphocyte+unicellular)), PMN#(apocyte
Number (neutrophil cell+basicyte+acidophil)), the monocyte ratio in MN%(leucocyte), in PMN%(leucocyte
Apocyte ratio) and measured value, unit respectively correspond display.Also chart is again provided with blood measuring in humoral determination to show
Area 114.Distribution map viewing area shows two distribution maps 115, and it is DIFF scatter plot that top, which disperses point diagram,.Lower part is divided into RBC
Use histogram.
Figure 15 be in Figure 14 picture 110 in the label 112 selection " retrieval BF(Research(BF)) example of label
Show.This picture also shows project same as picture 110 in addition to showing retrieval parameter viewing area 116.Retrieval parameter viewing area
Shown in 116 be present in as shown in Figure 10 the population " HF-BF# " of region HF, the population in the HF of region and be present in including
The ratio between the population in region including region HF and region MF " HF-BF% ", it is present in including region HF and region MF
The population " TC-BF# " in region.In addition, " HF-BF% " is the ratio between HF-BF and TC-BF.
Figure 16 is the storage sample guide look display picture 140 being shown on 3 display 302 of data processing equipment.130 be to suffer from
Person's attribute display area.Its top is equipped with the measurement result viewing area that display measurement result is selected by label.Measurement result is shown
Area's leftmost column 131 is for showing that the verifying work of measurement result is not done or done.V expression has verified that.Its right column 132 is for showing
Show mode determination.The measurement result of " F " expression humoral determination mode.Although if needing blank to examine under humoral determination mode
The high level sample of survey, but if not carrying out blank detection, it, can be by F inversion marks in order to be showed.
Above with regard to the structure and function of cellanalyzer of the invention, carried out for being previously charged into cellanalyzer
Explanation, but can also realize the function by control system, which is packed into traditional cellanalyzer, is allowed
Traditional cellanalyzer plays function of the invention.
In the structure described in present embodiment, to dialogue under leukocyte differential count and humoral determination mode under blood measuring mode
Specimen amount, reagent type and amount of reagent when cell classification respectively prepares measurement sample are all.Can be without being limited thereto, it can also allow
The specimen amount and amount of reagent for preparing the measurement sample of classification leucocyte under humoral determination mode are more than respectively prepares blood measuring mould
The specimen amount and amount of reagent of classification leucocyte measurement sample under formula.When due to being measured under humoral determination mode to leukocyte differential count
Between it is longer than blood measuring mode, measure that required measurement sample size is also more, and therefore, doing so can be respectively in blood measuring mode
Under leukocyte differential count and humoral determination mode under leukocyte differential count prepare suitable measurement sample.
In the present embodiment, with regard to being carried out under humoral determination mode to the structure of leukocyte differential count with scattering light and fluorescence
It illustrates, but not limited to this, with such as scattering light and light can also be absorbed under the humoral determination mode to leukocyte differential count.It absorbs
The coloring agent of stain leukocytes can be mixed into sample, preparation measurement sample, by the measurement by the measurement of light together with other reagents
Sample is supplied to flow cell, is allowed to form sample stream in flow cell, and the illumination sample stream passes through the light harvestings such as photodiode member
Part receives the light that sample stream issues.When leucocyte is by flow cell, light is absorbed by leucocyte, and degree of absorption can be used as collection
The collection light quantity of optical element is captured.About this light absorbing measurement, U.S. Patent No. 5122453 and U.S. Patent No.
It is delivered on No. 5138181 bulletins.Resistance can also be measured and replace scattering light, by resistance value and absorb light come to leucocyte progress
Classification.
Claims (9)
1. a kind of cellanalyzer, comprising:
Fluid device has and aspirates the aspirating specimen mouth of sample, and mix by it is described aspirate the sample that mouth aspirates and
Reagent prepares measurement sample；
Detection part is capable of detecting when scattering light that cell from the measurement sample for flowing through sheath flow pool obtains and glimmering
Light；
Controller handles the measurement result exported from the detection part and obtains analysis result；And
Display；
Wherein, the controller controls the display and shows for receiving the choosing to blood measuring mode and humoral determination mode
The input picture selected；
If having selected the blood measuring mode, sample is put into mode at least and can specify following mode: operator
Specimen container is inserted into the aspirating specimen mouth manually and aspirates the 1st mode of sample and by transporting the sample automatically
The conveyer supply sample of container and the 2nd mode that sample is aspirated by the aspirating specimen mouth；
If having selected the humoral determination mode, the 1st mode can only be specified as sample and be put into mode；
Wherein, the leucocyte in the blood measuring mode in classification blood,
The leucocyte in body fluid in the humoral determination mode other than classification blood.
2. cellanalyzer according to claim 1, it is characterised in that:
If having selected the blood measuring mode, sample is put into mode and can also specify following mode: being inhaled by sample
Move the 3rd mode for measuring sample that mouth aspirates the prior compound sample of operator and reagent and prepares.
3. cellanalyzer according to claim 1, it is characterised in that:
The controller controls the display and shows the input for being put into mode, mode determination and species of samples that can select sample
Picture；
In the input picture, blood and body fluid can choose as species of samples.
4. cellanalyzer according to claim 3, it is characterised in that:
In the input picture, the blood measuring mode is set to initial launch mode.
5. cellanalyzer according to claim 1, it is characterised in that:
If having selected the humoral determination mode and having aspirated humoral specimen by the 1st mode, the fluid device is certainly
It is dynamic to mix the humoral specimen and the reagent to prepare measurement sample.
6. cellanalyzer according to claim 1, it is characterised in that:
The fluid device after the humoral determination mode has been selected to prepare the reagent used when leukocyte differential count measurement sample
With selected the reagent used when the fluid device prepares leukocyte differential count measurement sample after the blood measuring mode to be
Similarly.
7. cellanalyzer according to claim 6, it is characterised in that:
Surfactant of the leukocyte differential count with reagent comprising lysed erythrocyte.
8. cellanalyzer according to claim 6, it is characterised in that:
Leukocyte differential count reagent includes polymethine class pigment.
9. cellanalyzer according to claim 1, it is characterised in that:
After being the humoral determination mode from the blood measuring pattern switching, the controller controls the blood cell analysis
Instrument is measured the blank detection of blank sample.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007022524 | 2007-02-01 | ||
| JP2007-022524 | 2007-02-01 | ||
| JP2007-119012 | 2007-04-27 | ||
| JP2007119012A JP4926812B2 (en) | 2007-02-01 | 2007-04-27 | Blood cell analyzer and body fluid analysis method |
| CN200810005239.2A CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810005239.2A Division CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105807038A CN105807038A (en) | 2016-07-27 |
| CN105807038B true CN105807038B (en) | 2019-06-18 |
Family
ID=39785791
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610208992.6A Active CN105807037B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610209662.9A Active CN105807038B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN200810005239.2A Active CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
| CN201610212407.XA Active CN105891090B (en) | 2007-02-01 | 2008-01-31 | Blood cell analyzer, body fluid analysis method, and control system therefor |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610208992.6A Active CN105807037B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810005239.2A Active CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
| CN201610212407.XA Active CN105891090B (en) | 2007-02-01 | 2008-01-31 | Blood cell analyzer, body fluid analysis method, and control system therefor |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP4926812B2 (en) |
| CN (4) | CN105807037B (en) |
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- 2008-01-31 CN CN200810005239.2A patent/CN101236195B/en active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| CN101236195A (en) | 2008-08-06 |
| CN101236195B (en) | 2016-05-04 |
| CN105891090A (en) | 2016-08-24 |
| CN105807037A (en) | 2016-07-27 |
| CN105807038A (en) | 2016-07-27 |
| JP2008209386A (en) | 2008-09-11 |
| CN105807037B (en) | 2019-05-28 |
| JP4926812B2 (en) | 2012-05-09 |
| CN105891090B (en) | 2020-01-17 |
| CN105866012A (en) | 2016-08-17 |
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