CN112881703B

CN112881703B - Blood detection device and method and computer storage medium

Info

Publication number: CN112881703B
Application number: CN201911209248.8A
Authority: CN
Inventors: 汤诚鹏; 许焕樟; 侯鹏飞
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2019-11-30
Filing date: 2019-11-30
Publication date: 2024-04-19
Anticipated expiration: 2039-11-30
Also published as: CN112881703A

Abstract

The application discloses a blood detection device and method, and a computer storage medium, wherein the blood detection device comprises: a plurality of detection channels including a blood routine detection channel and at least two specific protein detection channels; and the controller is connected with the plurality of detection channels and is used for controlling the plurality of detection channels to simultaneously perform blood routine detection and/or specific protein detection on the blood sample to be detected when the blood routine detection and/or specific protein detection is performed on the same blood sample to be detected, and controlling the first detection step and the second detection step to be simultaneously performed when an operation component required by a first detection step which is being executed or is to be executed by one detection channel in the plurality of detection channels is different from an operation component required by a second detection step which is to be executed by the other detection channel. By the method, on one hand, blood routine detection and a plurality of specific proteins can be realized on the same device, and on the other hand, detection efficiency can be improved through time sequence superposition.

Description

Blood detection device and method and computer storage medium

Technical Field

The present application relates to the field of blood testing technology, and in particular, to a blood testing device and method, and a computer storage medium.

Background

In clinical examination in hospitals, a patient is generally subjected to blood sampling and examination, and in general, blood routine examination and detection of various specific proteins are simultaneously carried out, and the two detection results are combined to comprehensively judge the illness state of the patient.

Blood routine is the most general, most basic blood test. Blood consists of two major parts, liquid and tangible cells, and is routinely examined for the cellular part of blood. The blood has three different functions, red blood cells, white blood cells and platelets. And judging the disease by observing the quantity change and the morphological distribution.

Specific protein assays are typically performed on proteins in the blood, such as C-reactive protein (CRP), serum Amyloid A (SAA), transferrin, and the like.

In the prior art, the two types of detection are respectively completed by adopting different devices, so that the operation is complicated on one hand, and multiple blood sample collection needs to be carried out on a patient on the other hand.

Disclosure of Invention

In order to solve the above problems, the present application provides a blood testing device and method, and a computer storage medium, which can realize routine blood testing and multiple specific protein testing on the same device, and can improve testing efficiency through time sequence superposition.

The application adopts a technical scheme that: provided is a blood test apparatus including: a plurality of detection channels including a blood routine detection channel and at least two specific protein detection channels; and the controller is connected with the plurality of detection channels and is used for controlling the plurality of detection channels to simultaneously perform blood routine detection and/or specific protein detection on the blood sample to be detected when the blood routine detection and/or specific protein detection is performed on the same blood sample to be detected, wherein when an operation component required by a first detection step which is being executed or is to be executed by one detection channel in the plurality of detection channels is different from an operation component required by a second detection step which is to be executed by the other detection channel, the first detection step and the second detection step are controlled to be executed simultaneously.

The application adopts another technical scheme that: there is provided a blood test method applied to a blood test apparatus as described above, the method comprising: collecting a blood sample to be detected; controlling a plurality of detection channels to simultaneously and respectively detect blood routine and/or specific proteins of the blood sample to be detected; wherein the plurality of detection channels includes a blood routine detection channel and at least two specific protein detection channels, and the first detection step and the second detection step are controlled to be performed simultaneously when an operational component required for a first detection step being performed or to be performed by one of the plurality of detection channels is different from an operational component required for a second detection step to be performed by another detection channel.

The application adopts another technical scheme that: there is provided a computer storage medium having stored therein program data for carrying out the method as described above when executed by a controller.

The blood test apparatus provided by the present application comprises: a plurality of detection channels including a blood routine detection channel and at least two specific protein detection channels; and the controller is connected with the plurality of detection channels and is used for controlling the plurality of detection channels to simultaneously perform blood routine detection and/or specific protein detection on the blood sample to be detected when the blood routine detection and/or specific protein detection is performed on the same blood sample to be detected, wherein when an operation component required by a first detection step which is being executed or is to be executed by one detection channel in the plurality of detection channels is different from an operation component required by a second detection step which is to be executed by the other detection channel, the first detection step and the second detection step are controlled to be executed simultaneously. Through the mode, the blood routine detection and the multiple specific protein detection are respectively carried out on the same blood sample to be detected through the multiple channels, so that the blood routine detection and the multiple specific protein detection can be carried out on one device, the detection efficiency is improved, the trouble to a patient caused by multiple sampling is avoided, and on the other hand, the multiple detection steps can be simultaneously carried out through a time sequence superposition mode, so that the detection speed is further accelerated.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:

FIG. 1 is a schematic view of a first embodiment of a blood test apparatus according to the present application;

FIG. 2 is a schematic diagram of a first timing diagram of a first embodiment of a blood test apparatus according to the present application;

FIG. 3 is a second timing diagram of a first embodiment of a blood test apparatus according to the present application;

FIG. 4 is a schematic view of a second embodiment of a blood test apparatus according to the present application;

FIG. 5 is a schematic view of a third embodiment of a blood test apparatus according to the present application;

FIG. 6 is a schematic view of a fourth embodiment of a blood test apparatus according to the present application;

FIG. 7 is a schematic flow chart of a first embodiment of a blood testing method according to the present application;

FIG. 8 is a schematic diagram showing a first process of detecting a plurality of specific proteins according to the present embodiment;

FIG. 9 is a schematic diagram showing a second flow path of detection of a plurality of specific proteins according to the present embodiment;

FIG. 10 is a schematic flow chart of routine blood detection provided in this embodiment;

FIG. 11 is a timing diagram of a first embodiment of a blood testing method according to the present application;

FIG. 12 is a flow chart of a second embodiment of a blood testing method provided by the present application;

FIG. 13 is a flow chart of a third embodiment of a blood testing method provided by the present application;

FIG. 14 is a flow chart of a fourth embodiment of a blood testing method provided by the present application;

FIG. 15 is a timing diagram of a fourth embodiment of a blood testing method according to the present application;

FIG. 16 is a flow chart of a fifth embodiment of a blood testing method provided by the present application;

FIG. 17 is a timing diagram of a fifth embodiment of a blood testing method according to the present application;

fig. 18 is a schematic structural diagram of an embodiment of a computer storage medium according to the present application.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. It is to be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present application are shown in the drawings. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

The terms "first," "second," and the like in this disclosure are used for distinguishing between different objects and not for describing a particular sequential order. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

Referring to fig. 1, fig. 1 is a schematic structural diagram of a first embodiment of a blood testing device according to the present application, and the blood testing device 10 includes a plurality of testing channels 11 and a controller 12 connected to the testing channels 11.

In one embodiment, the controller 12 is configured to control the plurality of detection channels 11 to simultaneously perform the blood routine detection and/or the specific protein detection on the same blood sample to be detected, respectively, wherein the first detection step and the second detection step are controlled to be performed simultaneously when an operation component required for a first detection step being performed or to be performed by one detection channel of the plurality of detection channels 11 is different from an operation component required for a second detection step to be performed by another detection channel.

It will be appreciated that the superposition of the detection steps is mainly taken into account if the different steps require the use of the same operating components. Taking blood separation operation, reagent adding and mixing operation, detection operation and cleaning operation as examples, wherein the blood separation operation mainly uses a blood separation and sample adding assembly (sampling needle), the reagent adding and mixing operation mainly uses a power device (such as a syringe or a valve), the detection operation mainly uses a detection assembly and a related pipeline or valve, and the cleaning operation mainly uses devices such as a syringe/air source/pump, a pipeline or a valve and the like. Therefore, if the operation components used in the detection steps of the two channels are different, the two channels can be synchronously executed.

It can be appreciated that the present embodiment is used for respectively performing different tests on the same blood sample to be tested, after collecting the blood sample of the patient, the collected blood sample to be tested is sequentially distributed to different channels through the sampling and blood-separating assembly, and then each test channel sequentially performs test on the distributed blood sample.

Optionally, when each detection channel detects a blood sample, part of the detection operations are overlapped to improve the detection efficiency.

As shown in fig. 2, in a practical example, the specific protein detection or blood routine detection includes step a, step B, step C and step D, and since the same step needs to use the same components, different detection channels cannot simultaneously perform the same step, for example, a blood separation operation, which may use a sampling blood separation component, and when the sampling blood separation component is a sampling needle, it is impossible to simultaneously perform a blood separation operation on a second detection channel when the sampling needle performs a blood separation operation on a first detection channel. Thus, while the first detection channel is executing step a, the other pauses for waiting; when the first detection channel executes the step B, the second detection channel executes the step A; when the first detection channel executes the step C, the second detection channel executes the step B, and the third detection channel executes the step A; when the first detection channel executes the step D, the second detection channel executes the step C, the third detection channel executes the step B, and the fourth detection channel executes the step A; and so on.

In another embodiment, the controller 12 is further configured to control the plurality of detection channels 11 to simultaneously perform the blood routine detection and/or the specific protein detection on the plurality of blood samples to be detected when the continuous blood routine detection and/or the specific protein detection is performed on the plurality of blood samples to be detected, wherein the first detection step and the second detection step are controlled to be performed simultaneously when an operation component required for a first detection step being performed or to be performed by one detection channel of the plurality of detection channels 11 is different from an operation component required for a second detection step to be performed by another detection channel.

Further, as shown in fig. 3, if the blood sample to be detected needs to be detected a and detected B simultaneously, and the time of detecting a is longer than the time of detecting B, then detecting a of blood sample 1 may be performed in the first channel, and detecting B of blood sample 1 may be performed in the second channel simultaneously (the timing sequence of detecting a of blood sample 1 and detecting B of blood sample 1 may be overlapped with each other as in the embodiment of fig. 2), then detecting a of blood sample 2 may be performed in the second channel after detecting B of blood sample 1 is completed, and detecting B of blood sample 2 may be performed in the first channel after detecting a of blood sample 1 is completed, so that detecting a and detecting B may be performed alternately through the two detecting channels, which may improve the detection efficiency.

Of course, the above embodiments are merely examples, and are not limiting of the number of detection channels, and the detection items may be different for each detection channel, and the steps performed may be different.

Referring to fig. 4, fig. 4 is a schematic structural diagram of a second embodiment of a blood testing device according to the present application, where the blood testing device 10 includes a plurality of testing channels 11 and a controller 12 connected to the testing channels 11, and in fig. 4, a liquid path is represented by a solid line, and a control line is represented by a dotted line.

The blood test apparatus 10 further includes a sampling blood separation unit 13, a power unit 14, a reagent container 15, and a waste liquid reservoir 16, and each test channel 11 includes a mixing reservoir 111, a test unit 112, a reagent liquid path 113, a cleaning liquid path 114, and a mixing liquid path (not shown).

The sampling and blood separating assembly 13 includes two functions of sampling and blood separating, specifically, the sampling and blood separating assembly 13 is used for collecting a blood sample to be detected and distributing the collected blood sample to be detected to the plurality of detection channels 11 according to a set sequence.

Optionally, in another embodiment, the blood testing device 10 further comprises a swab assembly coupled to the controller 12, the controller 12 further configured to control the sampling blood separation assembly 13 to perform a blood-throwing operation within the swab assembly. Specifically, the swab component can be of a cylindrical structure, the swab component is provided with a liquid outlet channel and a liquid inlet channel, the liquid inlet channel is connected with a power component such as an injector, and cleaning liquid such as diluent is injected into the swab through the power component such as the injector; the liquid outlet channel of the swab is connected with a liquid pump and other components, liquid in the swab is pumped out of the liquid outlet channel through the liquid pump, and when the blood sampling and separating assembly needs to throw blood, the blood sampling and separating assembly moves to the vicinity of the liquid outlet channel of the swab, and the blood sample is discharged through the liquid outlet channel of the swab. The controller 12 is used for controlling the sampling blood separation assembly 13 to axially move relative to the swab assembly so as to clean the outer wall of the sampling blood separation assembly 13 through the swab assembly.

Wherein the power device 13 is respectively connected to the reagent liquid path 113 and the cleaning liquid path 114 to provide power support for the liquid flow between the two containers connected by the liquid path.

Specifically, the reagent liquid path 113 connects the reagent container 15, the mixing tank 111 and the power device 14, and is used for performing reagent adding operation to the mixing tank 111 under the action of the power device 14; the mixing liquid path is connected with the mixing pool 111 and the power device 14 and is used for carrying out mixing operation on blood samples and/or reagents in the mixing pool 111 under the action of the power device 14; the cleaning liquid path 114 is connected to the mixing tank 111, the detection unit 112, the waste liquid tank 16 and the power unit 14, and is used for discharging the waste liquid in the mixing tank 111 and the detection unit 112 under the action of the power unit 14.

The reagent vessel 15 may include various reagent vessels such as a diluent vessel, a hemolyzing agent vessel, an antibody reagent, a washing liquid, and the like.

It will be appreciated that in the above embodiments, the reagent vessel 15 and the waste liquid tank 16 are common to a plurality of detection channels 11, and in other embodiments, each detection channel 11 may comprise one reagent vessel 15 and waste liquid tank 16, respectively.

In another embodiment, the blood detection device includes a power device, a heating device, and a plurality of detection channels, each detection channel includes a preheating tank, a mixing tank (X1, X2, X3), a detection assembly, a first pipeline T182, a second pipeline T99, a third pipeline T102, a fourth pipeline T101, and a fifth pipeline T39, and a sixth pipeline T98 may be additionally disposed in each path of detection liquid according to actual needs. Wherein the plurality of detection liquid paths includes at least two detection liquid paths, the following description will be given with reference to the two detection liquid paths shown in fig. 5 and 6.

The liquid power device is used for carrying out reagent adding operation and uniform mixing operation; the heating device is used for heating at least one of the mixing pool (X1, X2 and X3), the detection assembly, the second pipeline T99, the third pipeline T102, the fourth pipeline T101, the fifth pipeline T39 and the sixth pipeline T98; a first pipeline T182 connects the waste liquid collecting device WC1 with the mixing tanks (X1, X2 and X3) and is used for discharging waste liquid to the waste liquid collecting device WC; the second pipeline T99 is connected with the mixing tanks (X1, X2 and X3) and is used for adding a hemolysis reagent into the mixing tanks (X1, X2 and X3) through a hydrodynamic device; the third pipeline T102 is connected with the mixing tanks (X1, X2 and X3) and is used for adding antibody reagent into the mixing tanks (X1, X2 and X3) through a hydrodynamic device; a fourth pipeline T101 connects the mixing tanks (X1, X2 and X3) with a hydrodynamic device and is used for carrying out mixing operation through the hydrodynamic device; the detection assembly is used for detecting the evenly mixed reagent, the mixing tanks (X1, X2 and X3) and the detection tanks in the detection assembly are integrally arranged or separately arranged (the integrally arranged means that the mixing tanks are canceled to directly utilize the detection tanks in the detection assembly to bear the evenly mixing function), and the preheating tank is connected to the second pipeline and used for preheating the hemolysis reagent; the fifth pipeline T39 is connected with the fourth pipeline T101 and is used for providing diluent for the mixing tanks (X1, X2 and X3) and the detection assembly through the hydrodynamic device; the sixth pipeline T98 is connected with the fourth pipeline T101 and is used for supplying cleaning liquid to the mixing tanks (X1, X2 and X3) and the detection assembly through the hydrodynamic device.

Specifically, the hydrodynamic device includes a plurality of hydrodynamic devices, which may be of the type of a fixed displacement pump, a syringe, a gas source, etc., and is connected to a detection fluid path through an electromagnetic valve, for example, fixed displacement pumps DP04, DP05, DP06 for hemolysis reagent operation in fig. 5, fixed displacement pumps DP01, DP02, DP03 for antibody reagent operation, a pressure source connected to a dilution tank DIL-MR for dilution liquid supply, a fixed displacement pump DP07 for cleaning liquid supply, a mixing syringe for mixing operation, a hemolysis reagent syringe 1, a hemolysis reagent syringe 2, a hemolysis reagent syringe 3 for hemolysis reagent operation in fig. 6, an antibody liquid syringe 1, an antibody liquid syringe 2, an antibody liquid syringe 3 for antibody reagent operation, a mixing syringe IR for dilution liquid supply in fig. 5, a mixing syringe IR for cleaning liquid supply, a mixing syringe for mixing operation in fig. 6, a mixing syringe for IR supply operation and a mixing operation in the embodiment shown in fig. 6, and a volume of the syringe can be reduced by increasing the volume of the valves 16 in the fluid path. It will be readily understood by those skilled in the art that the type and use of the fluid power apparatus is not limited to that shown in fig. 5 and 6, and that those skilled in the art may arbitrarily combine to replace the dosing pumps DP04, DP05, DP06 for hemolysis reagent operation in fig. 5 with syringes, to replace the cleaning liquid syringe for performing cleaning liquid supply in fig. 6 with dosing pumps, etc.

Wherein the number of the hydrodynamic devices used for carrying out the mixing operation is N, the number of the multipath detection liquid paths is N,

When n=1, the mixing operation of the multiple detection liquid paths multiplexes the same hydrodynamic device, for example, as shown in fig. 1, the mixing operation of the 3 detection liquid paths multiplexes the mixing syringe IR.

When n=n, the mixing operation of each path of the detection liquid path separately uses one hydrodynamic device, for example, as shown in fig. 2, and the mixing operation of 3 paths of the detection liquid path separately uses a mixing syringe IR1, a mixing syringe IR2, and a mixing syringe IR3, respectively.

When 1< N, at least part of the mixing operation of the detection liquid paths is multiplexed with the same hydrodynamic device, for example, when n=6, n=3,

Each hydrodynamic device for carrying out mixing operation can be divided into two paths of detection liquid paths.

Or one path of detection liquid path of the first liquid power device pipe for carrying out the mixing operation, one path of detection liquid path of the second liquid power device pipe for carrying out the mixing operation and four paths of detection liquid path of the third liquid power device pipe for carrying out the mixing operation.

Or the first liquid power device pipe for carrying out the mixing operation is provided with one path of detection liquid path, the second liquid power device pipe for carrying out the mixing operation is provided with two paths of detection liquid paths, and the third liquid power device pipe for carrying out the mixing operation is provided with three paths of detection liquid paths.

The multiplex assay solution can be used to detect the same specific protein, which can be C-reactive protein, serum amyloid A or transferrin; or multiple detection lanes for detecting different species of a particular protein, e.g., in one embodiment, at least one detection lane is used to detect C-reactive protein (CRP) and at least one other detection lane is used to detect Serum Amyloid A (SAA).

In the embodiment of the invention, the heating device comprises a plurality of heating components with different temperatures, and the heating components are used for independently controlling or combining the temperatures of the preheating tank, the mixing tank (X1, X2 and X3), the detection component, the second pipeline T99, the third pipeline T102, the fourth pipeline T101 and/or the fifth pipeline T39. Independent temperature control can be understood as how many objects to be heated have the same number of heating components, one-to-one correspondence is used for independent heating, combined temperature control can be understood as that at least two heating objects share one heating component, for example, the temperatures of a preheating tank and a mixing tank (X1, X2 and X3) are relatively similar, and the same heating components can be reused.

The heating component is a heating film or a heating rod, the specific protein detection module further comprises a plurality of heat conducting matrixes for conducting heat to the preheating pool, the mixing pool (X1, X2 and X3), the detection component, the second pipeline T99, the third pipeline T102, the fourth pipeline T101 and/or the fifth pipeline T39, the heat conducting matrixes are in a block shape or a cylinder shape, the heating film is attached to the heat conducting matrixes, or the heating rod is inserted into the heat conducting matrixes, and the second pipeline T99, the third pipeline T102, the fourth pipeline T101 and/or the fifth pipeline T39 are wound on the cylinder-shaped heat conducting matrixes.

In an embodiment, the heating device includes a diluent heating tank, and the diluent heating tank is connected to the fifth pipeline T39 through the pipeline T120 and the pipeline T83 and is disposed in parallel with the hydrodynamic device for performing the mixing operation relative to the fourth pipeline T101, so that bubbles generated by heating the diluent heating tank can not affect the detection accuracy, which is beneficial to improving the overall stability of the detection.

The mixing detection assembly comprises a base body, a detection pool arranged on the base body, and a laser and a signal receiver which are arranged on the base body and positioned on two sides of the detection pool, wherein the detection pool is communicated with the mixing pool (X1, X2 and X3).

Or the mixing detection assembly comprises a matrix, a laser and a signal receiver, wherein the mixing tanks (X1, X2 and X3) are arranged on the matrix, and the laser and the signal receiver are arranged on the matrix and are positioned on two sides of the mixing tanks (X1, X2 and X3).

As shown in fig. 5 and 6, the specific protein detection module further includes a plurality of first gate valves (SV 10, SV11, SV 12), a plurality of first gate valves (SV 04, SV05, SV 06), a plurality of third gate valves (SV 01, SV02, SV 03), a plurality of confluence joints (J2, J3), and a confluence line T199.

The first line T182 connects the mixing tanks (X1, X2, X3) with the confluence joints (J2, J3), the first gate valve (SV 10, SV11, SV 12) is connected to the first line T182, and the confluence line T199 connects the plurality of confluence joints (J2, J3) in series and to the waste liquid collecting device WC1.

The second pipeline comprises a second public pipeline T106, a second liquid pushing pipeline (T97, T99) and a second liquid sucking pipeline (T95, T94), the public end of the first gating valve (SV 04, SV05, SV 06) is connected with a liquid power device (a dosing pump DP04, DP05, DP 06) for adding reagents through the second public pipeline T106, one sub-port of the first gating valve (SV 04, SV05, SV 06) is connected with a mixing pool (X1, X2, X3) through the second liquid pushing pipeline (T97, T99), a preheating pool is connected to the second liquid sucking pipeline (T95, T94) and the other sub-port of the first gating valve (SV 04, SV05, SV 06) through the second liquid pushing pipeline (T97, T99), an optical coupler is arranged on the path of the second liquid sucking pipeline (T95, T94) for detecting whether liquid exists or not.

The third pipeline comprises a third public pipeline T106, a third liquid pushing pipeline T102 and a third liquid sucking pipeline T103, the third public pipeline T106 is used for connecting a public end of a third gating valve (SV 01, SV02 and SV 03) and a hydrodynamic device (dosing pumps DP01, DP02 and DP 03) for adding reagents, the third liquid pushing pipeline T102 is used for connecting one sub-port of the third gating valve (SV 01, SV02 and SV 03) with a mixing pool (X1, X2 and X3), the third liquid sucking pipeline T103 is connected with the other sub-port of the third gating valve (SV 01, SV02 and SV 03) and is connected with an anti-body fluid reagent bottle, and an optical coupler is arranged on a path of the third liquid sucking pipeline T103 so as to detect whether liquid exists or not.

As shown in fig. 5, the specific protein detection module further comprises a fourth gating valve SV09, a fifth gating valve SV08, a sixth gating valve SV07, a plurality of seventh gating valves (SV 10, SV11, SV 12), a first three-way joint J3, a second three-way joint J3, and a plurality of branching joints (J3, J3).

The sixth pipeline comprises a sixth first pipeline T106, a sixth second pipeline T98 and a sixth third pipeline (T95 and T94), the sixth first pipeline T106 is used for connecting the public end of the sixth gating valve SV07 with a liquid power device (a constant delivery pump DP 07) for adding reagents, the sixth second pipeline T98 is connected between one sub-port of the sixth gating valve SV07 and the first port of the first three-way joint J3, the sixth third pipeline (T95 and T94) is connected with the other sub-port of the sixth gating valve SV07 and is connected with a cleaning liquid reagent bottle, and an optical coupler is arranged on the sixth third pipeline (T95 and T94) for detecting whether liquid exists or not.

The fifth pipeline comprises a fifth first pipeline T39, a fifth second pipeline (T120 and T83) and a fifth third pipeline T12, the fifth first pipeline T39 connects the public end of a fifth strobe valve SV08 with a diluent tank DIL-MR, the fifth second pipeline (T120 and T83) is connected between one branch port of the fifth strobe valve SV08 and a second port of a first three-way joint J3, the fifth third pipeline T12 is connected between the other branch port of the fifth strobe valve SV08 and the first port of a second three-way joint J3, and the second port of the second three-way joint J3 is connected with a hydrodynamic device (a mixing syringe IR) for mixing;

The fourth pipeline comprises a fourth first pipeline T100, a fourth second pipeline T1 and a fourth third pipeline T69, the fourth first pipeline T100 connects the public end of the fourth strobe valve SV09 with a plurality of mixing pools (X1, X2 and X3) through a plurality of branch connectors (J3 and J3), a plurality of seventh strobe valves (SV 10, SV11 and SV 12) are connected on the pipelines between the mixing pools (X1, X2 and X3) and the branch connectors (J3 and J3), the fourth second pipeline T1 is connected between one branch port of the fourth strobe valve SV09 and a third port of the first three-way connector J3, and the fourth third pipeline T69 is connected between the other branch port of the fourth strobe valve SV09 and a third port of the second three-way connector J3.

As shown in fig. 6, the specific protein detection module further comprises a fourth gating valve SV09, a fifth gating valve SV08, a sixth gating valve SV07, a plurality of seventh gating valves (SV 10, SV11, SV 12), a first three-way joint J3, a split joint (J3, J3);

The sixth pipeline includes a sixth first pipeline T106, a sixth second pipeline T98 and a sixth third pipeline (T95, T94), the sixth first pipeline T106 connects the common end of the sixth gate valve SV07 with the hydrodynamic device (dosing pump DP 07) for adding reagents, the sixth second pipeline T98 is connected between one of the sub-ports of the sixth gate valve SV07 and the first port of the first three-way joint J3, the sixth third pipeline (T95, T94) is connected with the other of the sub-ports of the sixth gate valve SV07, and an optocoupler is provided on the sixth third pipeline (T95, T94) to detect whether the liquid is present or not.

The fifth pipeline comprises a fifth first pipeline T39, a fifth second pipeline (T120, T83) and a fifth third pipeline T12, wherein the fifth first pipeline T39 is used for connecting the public end of the fifth strobe valve SV08 with a hydrodynamic device (a mixing syringe IR) for mixing evenly, the fifth second pipeline (T120, T83) is connected between one sub-port of the fifth strobe valve SV08 and the second port of the first three-way joint J3, the fifth third pipeline T12 is connected between the other sub-port of the fifth strobe valve SV08 and one sub-port of the fourth strobe valve SV09, and the third port of the first three-way joint J3 is connected with the other sub-port of the fourth strobe valve SV 09.

The common end of the fourth gating valve SV09 is connected with the mixing tanks (X1, X2 and X3) through a plurality of shunting joints (J3 and J3), and a plurality of seventh gating valves (SV 10, SV11 and SV 12) are connected on the pipelines between the mixing tanks (X1, X2 and X3) and the shunting joints (J3 and J3).

And a capacity expansion pipeline T101 is arranged between the seventh gating valves (SV 10, SV11 and SV 12) and the detection assembly, the capacity expansion pipeline T101 increases the buffer volume in a mode of expanding the pipeline diameter or increasing the pipeline length (such as multi-winding) so as to prevent the reagent from exceeding the split joints (J3 and J3) when the reagent is uniformly mixed, and if the reagent exceeds the split joints (J3 and J3), the liquid in the liquid pipeline is sent into other liquid pipelines, so that the detection precision is affected.

Referring to fig. 5, the specific flow path of the mixing tank X1 (hereinafter referred to as channel 1) during detection is as follows:

Step 1: opening an SV13 valve, emptying the original base solution (the liquid used for soaking the mixing tank X1, which can be diluent) in the mixing tank X1, controlling a quantitative pump DP06 and the SV06 valve, adding a specific protein hemolysis reagent into the mixing tank X1 to clean the mixing tank X1, and opening the SV13 valve again to empty the mixing tank X1;

Step 2: controlling the quantitative pumps DP06 and SV06 to add a specific protein hemolysis reagent into the mixing pool X1, and simultaneously controlling a sampling needle of the collection and distribution module to add a blood sample;

Step 3: opening an SV08 valve, an SV09 valve and an SV10 valve, controlling an IR back and forth suction and spitting of a mixing injector, fully mixing a hemolysis reagent and a blood sample, and preserving heat at a mixing pipeline T101 through a heating device, for example, adopting a cylindrical heat-conducting matrix with a heating rod arranged inside, wherein the mixing pipeline T101 is wound around the periphery of the cylindrical heat-conducting matrix, so that unstable reaction temperature caused by heat dissipation in the mixing process is avoided;

Step 4: after the hemolysis process is finished, controlling a dosing pump DP01 and an SV01 valve, adding a specific protein antibody reagent into a mixing pool X1, then opening an SV08 valve, an SV09 valve and an SV10 valve, controlling a mixing injector IR to suck and spit back and forth, fully mixing reaction liquid, and similarly, adding a heating device at the position of a mixing pipeline T101 for heat preservation, so as to avoid unstable reaction temperature caused by heat dissipation in the mixing process;

step 5: after the full mixing is finished, opening an SV08 valve, an SV09 valve and an SV10 valve, controlling a mixing injector IR to send the reaction liquid into a detection pool of a detection assembly, and starting detection;

step 6: after the detection is finished, an SV10 valve is opened, quantitative pumps DP07 and SV07 valves are controlled, and cleaning liquid is added into pipelines T98, T1, T100, T101 and T104;

Step 7: the operation of the cleaning method is simple, the cleaning time is shortened, the cleaning efficiency is enhanced, the cleaning strength of the cleaning liquid on specific substances is higher than that of the cleaning liquid on the specific substances, and the specific substances can be immunoreactive conjugates or reaction residues, compared with the cleaning mode of using a sampling needle to add the cleaning liquid into the mixing tank X1 and then using a mixing injector IR to pump the cleaning liquid from the mixing tank X1 into the detecting tank to clean, and then pushing the liquid in the detecting tank back into the mixing tank X1. After cleaning, opening SV13, emptying the mixing tank X1, and finally adding diluent into the mixing tank X1 to form base solution; the entering diluent needs to pass through a heating tank between an SV08 valve and a tee joint J3, so that the diluent is heated, the temperature of the liquid entering the detection tank is kept in a range, the influence on the reaction stability due to overlarge temperature difference of the detection tank is avoided, and when continuous detection is carried out, the temperature of the liquid entering the detection tank is not greatly reduced during cleaning because the diluent is heated, so that the next detection can be conveniently and rapidly started.

The specific flow of the counting detection in the liquid paths (called channels 2 and 3) where the mixing pool X2 and the mixing pool X3 are positioned is the same as the detection flow in the channel 1, when a plurality of channels detect the same specific protein, the pilot reagents of the access pipelines are the same to generate the same chemical reaction, and when a plurality of channels detect different specific proteins, the reagents in the access pipelines are different to generate different chemical reactions.

Referring to fig. 6, the specific flow of the liquid path (hereinafter referred to as channel 1) in which the mixing tank X1 is located during counting and detection is as follows:

Step 1: opening an SV13 valve, emptying the original base solution (the liquid used for soaking the mixing tank X1, which can be diluent) in the mixing tank X1, controlling a hemolysis agent injector 1 and the SV06 valve, adding a specific protein hemolysis reagent into the mixing tank X1 to clean the mixing tank X1, and opening the SV13 valve again to empty the mixing tank X1;

step 2: controlling a hemolysis agent injector 1 and an SV06 valve, adding a specific protein hemolysis reagent into a mixing pool X1, and simultaneously controlling a sampling needle of a collection and distribution module to add a blood sample;

step 3: opening an SV16 valve, an SV09 valve and an SV10 valve, controlling an IR of a mixing injector to suck and spit back and forth, fully mixing a hemolysis reagent and a blood sample, and preserving heat at the position of a mixing pipeline T101 through a heating device, for example, adopting a cylindrical heat conducting matrix with a heating rod arranged inside, wherein the mixing pipeline T101 is wound around the periphery of the cylindrical heat conducting matrix, so that unstable reaction temperature caused by heat dissipation in the mixing process is avoided;

Step 4: after the hemolysis process is finished, controlling an antibody liquid injector 1 and an SV01 valve, adding a specific protein antibody reagent into a mixing pool X1, then opening an SV16 valve, an SV09 valve and an SV10 valve, controlling an IR of the mixing injector to suck and spit back and forth, fully mixing reaction liquid, and similarly, keeping the temperature by a heating device at the position of a mixing pipeline T101 to avoid unstable reaction temperature caused by heat dissipation in the mixing process;

step 5: after the complete mixing, opening an SV16 valve, an SV09 valve and an SV10 valve, controlling a mixing injector IR to send the reaction liquid into a detection tank, and starting detection;

Step 6: after the detection is finished, opening an SV10 valve, controlling a cleaning liquid injector and an SV07 valve, and adding cleaning liquid into pipelines T98, T1, T100, T101 and T104; the SV08 valve, the SV10 valve and the SV16 valve are opened, the mixing injector IR is controlled to enable the diluent in the diluent pool DIL to enter the pipelines T39, T120 and T83 so as to push the cleaning liquid in the pipelines T1, T100, T101 and T104 to clean the mixing pipeline T101, the detection assembly and the mixing pool X1 together, the SV13 is opened after cleaning to empty the mixing pool X1, and the cleaning liquid is pushed into the detection assembly and the mixing pool X1 by the diluent, so that compared with the cleaning liquid is pumped into the mixing pool X1 by a sampling needle and then pumped into the detection pool by the mixing injector IR for cleaning, and finally the liquid in the detection pool is pushed back into the mixing pool X1 by the mixing injector IR, the cleaning mode has the advantages of simple operation of parts, shortened cleaning time and enhanced cleaning efficiency, wherein the cleaning strength of the cleaning liquid on specific substances, which can be immunoreactive conjugates or reactant, is larger than the cleaning strength of the diluent on the specific substances. After cleaning, opening SV13, emptying the mixing tank X1, and finally adding diluent into the mixing tank X1 to form base solution; the entering diluent needs to pass through a heating tank between an SV08 valve and a tee joint J3, so as to heat the diluent, keep the temperature of the liquid entering a detection tank in a range, and avoid the influence on the reaction stability due to overlarge temperature difference of the detection tank.

Optionally, in this embodiment, a plurality of specific protein detection channels are used to detect at least two of SAA, CRP, TRF (transferrin), hs-CRP (hypersensitive C-reactive protein), PCT (procalcitonin) and D-dimer, respectively, and a blood routine detection channel is used to detect wbcs (white blood cells) and rbcs (red blood cells).

Alternatively, in other embodiments, the plurality of specific protein detection channels may be 4, 5, 6, 7, or 8. In the case where a plurality of specific protein detection channels is 6, these 6 detection channels are used for detecting SAA (serum amyloid A), CRP (C-reactive protein), TRF (transferrin), hs-CRP (hypersensitive C-reactive protein), PCT (procalcitonin) and D-Dimer (D-Dimer), respectively. Because the principle of detection of the specific proteins is the same, the detection conditions are similar, each channel can be unbound to the detection item, and the same detection channel can be used for detecting different specific protein parameters at different moments.

The following describes, by way of an example, the superposition of the operating steps for the detection of different items of the same blood sample.

Referring to fig. 7, fig. 7 is a schematic flow chart of a first embodiment of a blood detection method according to the present application, the method includes:

Step 71: and collecting a blood sample to be detected.

Step 72: and controlling the plurality of detection channels to simultaneously and respectively detect blood routine detection and/or specific protein detection of the blood sample to be detected.

Wherein the first detection step and the second detection step are controlled to be executed simultaneously when an operation component required for the first detection step which is being executed or to be executed by one detection channel of the plurality of detection channels is different from an operation component required for the second detection step which is to be executed by the other detection channel.

It will be appreciated that in performing the timing stack, it is primarily considered whether the different steps require the use of the same operational components. Taking blood separation operation, reagent adding and mixing operation, detection operation and cleaning operation as examples, wherein the blood separation operation mainly uses a blood separation and sample adding assembly (sampling needle), the reagent adding and mixing operation mainly uses a power device (such as a syringe or a valve), the detection operation mainly uses a detection assembly and a related pipeline or valve, and the cleaning operation mainly uses devices such as a syringe/air source/pump, a pipeline or a valve and the like. Therefore, if the operation components used in the detection steps of the two channels are different, the two channels can be synchronously executed.

Alternatively, the detection in this embodiment includes blood routine detection and a plurality of specific protein detection, for example, blood routine detection may be performed first followed by a plurality of specific protein detection, or blood routine detection may be performed first followed by a plurality of specific protein detection.

Step 72 may specifically include: sequentially performing blood separation operation, reagent adding operation, uniform mixing operation, detection operation and cleaning operation on a plurality of specific protein detection channels; and (3) performing blood separation operation, reagent adding and mixing operation, detection operation and cleaning operation on the blood routine detection channel.

Referring now to fig. 8, fig. 8 is a schematic diagram illustrating a first process for detecting a plurality of specific proteins according to the present embodiment, the method includes:

Step 81: and controlling the sampling and blood separating assembly to move to a mixing pool of the target specific protein detection channel for blood separating operation.

Alternatively, when the target specific protein detection channel is a first specific protein detection channel of a plurality of specific protein detection channels, more specifically, when the detection time of a specific protein item detected by the first specific protein detection channel is longest, a blood separation operation is performed on the first specific protein detection channel. The method further comprises the following steps before the step 81: and controlling the sampling blood separating component to sample the blood sample to be detected, and controlling the sampling blood separating component to move to the vicinity of the liquid outlet end of the swab component to perform the operation of throwing blood in the swab.

Step 82: and controlling the first power device to add reagent to the mixing pool of the target specific protein detection channel for mixing, and simultaneously controlling the sampling blood separating component to move to the mixing pool of the next specific protein detection channel for blood separating operation.

Step 83: and controlling the first power device to suck the blood sample to be detected which is uniformly mixed in the mixing pool of the target specific protein detection channel into the detection assembly of the target specific protein detection channel, and simultaneously controlling the second power device to uniformly mix the reagent in the mixing pool of the next specific protein detection channel.

Step 84: and controlling the detection assembly of the target specific protein detection channel to detect the sucked blood sample to be detected, and simultaneously controlling the second power device to suck the blood sample to be detected which is uniformly mixed in the mixing pool of the next specific protein channel into the detection assembly of the next specific protein detection channel.

Step 85: and controlling the cleaning component to carry out cleaning operation on the mixing pool of the target specific protein detection channel, and simultaneously controlling the detection component of the next specific protein detection channel to carry out detection operation on the sucked blood sample to be detected.

Step 86: and (3) cleaning the mixing pool of the next specific protein detection channel.

In this embodiment, since the power units of the different detection channels are independent, if the power units are required to be used in the detection steps of both channels, the two steps may be performed simultaneously, for example, in step 83, the first power unit is controlled to suck the blood sample to be detected after being mixed in the mixing pool of the target specific protein detection channel into the detection component of the target specific protein detection channel, and the second power unit is controlled to perform reagent adding and mixing operations on the mixing pool of the next specific protein detection channel.

Referring now to fig. 9, fig. 9 is a schematic diagram illustrating a second flow chart of detection of a plurality of specific proteins according to the present embodiment, the method includes:

step 91: and controlling the sampling and blood separating assembly to move to a mixing pool of the target specific protein detection channel for blood separating operation.

Step 92: and controlling the power device to add reagent to the mixing pool of the target specific protein detection channel for mixing, after mixing, controlling the power device to pump the mixed sample into the detection assembly, and simultaneously controlling the sampling blood separation assembly to move to the mixing pool of the next specific protein detection channel for blood separation.

Step 93: the detection assembly is controlled to detect a sample to be detected, the power device is controlled to carry out reagent adding and mixing operation on a mixing pool of a next specific protein detection channel, and after mixing is completed, the power device is controlled to pump the well mixed sample into the detection pool.

Step 94: and controlling the cleaning component to carry out cleaning operation on the mixing pool of the target specific protein detection channel, and simultaneously controlling the detection component of the next specific protein detection channel to carry out detection operation on the sucked blood sample to be detected.

Step 95: and (3) cleaning the mixing pool of the next specific protein detection channel.

In this embodiment, since the same power device is multiplexed by different detection channels, if the power devices are required for both detection channels, the two steps cannot be performed simultaneously, for example, in steps 92 and 93, the power device of one detection channel is required to pump the well mixed sample into the detection assembly, and the reagent adding and mixing operation of the next detection channel is performed after the power device is not required.

Referring now to fig. 10, fig. 10 is a schematic flow chart of routine blood test provided in this embodiment, where the routine blood test method includes:

Step 101: and controlling the sampling and blood separating assembly to move to the WBC detection channel for blood separating operation.

Optionally, before step 101, the method may further include: and controlling the sampling blood separating component to move to the vicinity of the liquid outlet end of the swab component, and performing blood throwing operation in the swab.

Step 102: after the to-be-detected blood sample in the WBC detection channel is diluted, the sampling and blood separating component is controlled to perform a sample sucking operation on the to-be-detected blood sample in the WBC detection channel.

Step 103: the power device is controlled to perform the first reagent adding and uniformly mixing operation on the WBC detection channel, and the sampling and blood separating assembly is controlled to move to the RBC detection channel to perform the blood separating operation.

Step 104: and controlling the power device to perform secondary reagent adding and mixing operation on the WBC detection channel, and simultaneously controlling the power device to perform reagent adding and mixing operation on the RBC detection channel.

Step 105: and detecting the WBC detection channel and the RBC detection channel simultaneously.

Step 106: and (3) cleaning the WBC detection channel and the RBC detection channel simultaneously.

Referring to fig. 8 and 10, if a specific protein assay is performed first and then a blood routine assay is performed in one embodiment, the blood routine assay also allows for a time sequence overlay with the previous specific protein assay.

Specifically, when the last specific protein channel performs detection operation, controlling the sampling blood-separating component to perform sample sucking operation on a blood sample to be detected in the WBC detection channel; when the last specific protein channel is subjected to cleaning operation, the power device is controlled to perform the first reagent adding and mixing operation on the WBC detection channel, and meanwhile, the sampling and blood separating assembly is controlled to move to the RBC detection channel to perform blood separating operation.

Referring to fig. 8, 10 and 11, fig. 11 is a timing diagram of a first embodiment of the blood detection method according to the present application, and the above manner will be described by a specific embodiment, in this embodiment, a specific protein detection channel includes a SAA channel and a CRP channel, and a blood routine detection channel includes WBC and RBC channels.

The first step, a first sample suction time sequence is started, and the following actions are completed: the blood sampling and separating assembly (sampling needle) starts to absorb the blood sample to be detected, and after the blood sample to be detected is absorbed, the blood sampling and separating assembly is retracted to the upper end of the swab assembly, and the outer wall is cleaned in the rising process of the blood sampling and separating assembly; then, the blood throwing is completed in the swab assembly, so that the pollution of the previous operation to the blood sample is avoided; the sampling blood separating component moves to the upper part of the SAA channel in the blood throwing process;

Next, start the "SAA channel blood separation" sequence, complete the following actions: the sampling blood-separating component enters an SAA mixing pool of an SAA channel, a blood sample to be detected is added into the SAA channel, and the blood sample and the SAA hemolytic agent are uniformly mixed by the action of a mixing injector 1; optionally, before this step, the operation of adding the hemolytic agent in the mixing tank of the SAA channel by the mixing syringe 1 may be performed first, and then the blood is separated, so as to complete the mixing of the blood sample and the hemolytic agent;

Next, start the "SAA channel mix" timing sequence, finish the following actions: uniformly mixing the hemolyzed blood sample in the SAA channel with SAA antibody liquid by a uniform mixing injector 1;

Simultaneously, a CRP channel blood separation time sequence is started to finish the following actions: the sampling blood-separating component enters a CRP mixing pool of a CRP channel, a blood sample to be detected is added into the CRP channel, and the blood sample to be detected and a CRP hemolytic agent are uniformly mixed by a mixing injector 2;

Next, the "SAA channel blood sample preparation" sequence is initiated, completing the following actions: pumping the reaction liquid which is uniformly mixed in the SAA channel into an SAA detection assembly from an SAA mixing pool through a mixing injector 1, and preparing for detection;

Simultaneously, starting a 'CRP channel mixing' time sequence to finish the following actions: uniformly mixing the hemolyzed blood sample in the CRP channel with CRP antibody liquid through a uniform mixing injector 2;

Next, starting an SAA channel detection time sequence, and starting to detect the blood sample in the SAA detection component;

Simultaneously, starting a 'CRP channel blood sample preparation' time sequence, pumping the reaction liquid which is uniformly mixed in the CRP channel into a CRP detection assembly from a CRP mixing pool through a mixing syringe 2, and preparing to start detection;

simultaneously, the sampling blood separating component completes throwing blood inside the swab;

Next, starting a 'CRP channel detection' time sequence, and starting to detect the blood sample in the CRP detection component;

Then separating blood by the WBC channel, after the blood separation is finished, performing secondary sample suction by the sampling and separating assembly, then sampling the blood separating assembly to move the RBC channel, adding the diluted blood sample to be detected into the RBC pool, and uniformly mixing the blood sample after the hemolytic agent is added into the WBC pool; next, the WBC/RBC count is started;

next, starting an SAA channel cleaning time sequence, and starting to clean the SAA channel;

Next, a "CRP channel wash" sequence is initiated to begin washing the CRP channel.

Compared with the prior art, the blood routine detection and the multiple specific protein detection can be carried out on the same blood sample to be detected through the multiple channels, so that the blood routine detection and the multiple specific protein detection can be carried out on one device, the detection efficiency is improved, the trouble to a patient caused by multiple sampling is avoided, and the multiple detection steps can be simultaneously carried out in an operation step superposition mode, so that the detection speed is further accelerated.

By way of one embodiment, a temporal superposition of different item detections of a plurality of consecutive blood samples is described below.

Referring to fig. 12, fig. 12 is a schematic flow chart of a second embodiment of a blood testing method according to the present application, the method includes:

step 121: blood samples were collected.

Step 122: and controlling the detection channels to simultaneously and respectively detect blood routine and/or specific proteins of a plurality of blood samples to be detected.

Wherein the plurality of detection channels comprises a blood routine detection channel and at least two specific protein detection channels, and when an operation component required by a first detection step which is being executed or to be executed by one detection channel in the plurality of detection channels is different from an operation component required by a second detection step which is to be executed by another detection channel, the first detection step and the second detection step are controlled to be executed simultaneously.

In the following, a description will be given by way of an embodiment in which a blood test apparatus includes a first test channel, a second test channel, and a third test channel, the test time of a first specific protein being shorter than the test time of a second specific protein.

Referring to fig. 13, fig. 13 is a schematic flow chart of a third embodiment of a blood detection method according to the present application, the method includes:

step 131: and controlling the sampling and blood separating assembly to perform sample sucking operation.

Step 132: and carrying out first specific protein detection on the current sample to be detected in the first detection channel, carrying out second specific protein detection on the current sample to be detected in the second detection channel, and carrying out blood routine detection on the current sample to be detected in the third detection channel.

Step 133: and after the blood routine detection of the current sample to be detected is completed, the next sample to be detected is subjected to the blood routine detection in a third detection channel.

As shown in fig. 3, if the blood sample to be detected needs to be detected a and detected B simultaneously, and the time of detecting a is longer than the time of detecting B, then detecting a of the blood sample 1 may be performed in the first channel, and detecting B of the blood sample 1 may be performed in the second channel simultaneously (the timing of detecting a of the blood sample 1 and detecting B of the blood sample 1 may be overlapped with each other as in the embodiment of fig. 2), then detecting a of the blood sample 2 may be performed in the second channel after the detecting B of the blood sample 1 is completed, and detecting B of the blood sample 2 may be performed in the first channel after the detecting a of the blood sample 1 is completed, so that detecting a and detecting B may be alternately performed through the two detecting channels, which may improve the detecting efficiency.

In an alternative embodiment, as shown in fig. 14, fig. 14 is a schematic flow chart of a fourth embodiment of a blood testing method according to the present application, where the method includes:

Step 141: and sequentially carrying out blood separation and mixing operation and detection operation on the current sample to be detected in the first detection channel.

Step 142: and when the first detection channel carries out detection operation on the current sample to be detected, the second detection channel carries out blood separation and mixing operation and detection operation on the current sample to be detected in sequence.

Step 143: and carrying out blood routine detection on the current sample to be detected in the third detection channel while carrying out detection operation on the second detection channel.

Wherein, can also include: and after the detection of the first specific protein of the current sample to be detected is finished, and after the blood separation and mixing operation in the conventional detection of the blood of the current sample to be detected is finished, carrying out the detection of the second specific protein on the next sample to be detected in the first detection channel.

Referring to fig. 15, fig. 15 is a timing diagram of a fourth embodiment of a blood testing method according to the present application, where the testing method may specifically be as follows:

1. the sampling needle completes the sample sucking process of the first sample in the sample sucking 1 time sequence section;

2. After the sample is sucked, the sampling needle moves to a first detection channel, the collected blood sample is distributed to the first detection channel, and after the reagent is added, the power device uniformly mixes the reagent in the first detection channel and the mixed liquid of the blood sample;

3. After the blood separation and mixing operation of the first detection channel is completed, the sampling needle is operated to the second detection channel, the blood sample is distributed to the second detection channel, and after the reagent is added, the power device is used for carrying out the mixing operation on the mixed solution of the reagent and the blood sample in the second detection channel; simultaneously, a first detection item (e.g. CRP) of a first detection channel starts detection, and two groups of operations are parallel;

4. after the blood separation and mixing operation of the second detection channel is completed, the second detection (SAA) item of the second detection channel starts to be detected; simultaneously, the sampling needle moves to a blood routine detection module to start blood routine detection, and two groups of operations are parallel;

5. When the blood routine detects a certain process, the sample suction of the second sample is started, and in order to make the detection time shorter, preferably, the first detection channel can immediately perform the blood separation operation of the second sample in the first detection channel after the CRP detection is completed;

6. Outputting a result after the first detection channel finishes crp detection, simultaneously sampling a blood separating assembly pair, moving the blood separating assembly pair to the upper part of the first detection channel, separating a blood sample of a second sample into the first detection channel, and uniformly mixing a reagent in the first detection channel and a mixed solution of the blood sample by a power device after adding a hemolytic agent and an antibody reagent for SAA;

7. Outputting a result after the SAA detection of the first sample is completed by the second detection channel, simultaneously sampling the SAA project of the first detection channel, moving the blood separating assembly pair to the upper part of the second detection channel, separating the blood sample of the second sample into the second detection channel, and after adding the hemolytic agent and the antibody agent for CRP, uniformly mixing the reagent in the first detection channel and the mixed solution of the blood sample by the power device, wherein the two operations are parallel;

8. After the blood separation and mixing action of the second detection channel is finished, the sampling blood separation assembly moves to the upper part of the blood routine detection channel to separate the blood of the blood routine detection channel, and meanwhile, CRP items of the second detection channel are detected, and the two operations are parallel;

9. when the blood routine detects a certain process, starting the sample suction of a third sample, and determining the superposition of the time sequence sample suction 3 and the time sequence of the blood routine detection 2 through the arrangement of the time sequences, so as to ensure that the blood separation of the third sample can be carried out immediately after the saa detection of the immune 1 channel is completed;

and repeating the steps 1-9, and completing continuous detection of a plurality of detection items corresponding to a plurality of samples.

It will be appreciated that in the embodiment of fig. 15, the "blood blending 11" timing and the "blood blending 21" timing cannot be superimposed since the two detection channels multiplex the same power device. In another embodiment, the two detection channels can use the respective power devices independently, so that the time sequence of 'blood separation and mixing' can be divided into two steps of 'blood separation' and 'mixing', and after the first detection channel finishes 'blood separation', the 'blood separation' and 'mixing' can be started in the second detection channel.

In another embodiment, the blood test device includes a first test channel, a second test channel, a third test channel, a fourth test channel, and a fifth test channel, wherein the test time for the first specific protein is less than the test time for the second specific protein. Step 121 may specifically include: carrying out first specific protein detection on a current sample to be detected in a first detection channel, carrying out second specific protein detection on the current sample to be detected in a second detection channel, carrying out first specific protein detection on a next sample to be detected in a third detection channel, carrying out second specific protein detection on the next sample to be detected in a fourth detection channel, and carrying out blood routine detection on the fifth detection through the current sample to be detected; wherein the first specific protein detection, the second specific protein detection, and the blood routine detection are performed simultaneously.

Specifically, as shown in fig. 16, fig. 16 is a schematic flow chart of a fifth embodiment of a blood detection method according to the present application, where the method includes:

Step 161: and sequentially carrying out blood separation and mixing operation and detection operation on the current sample to be detected in the first detection channel.

Step 162: and when the first detection channel carries out detection operation on the current sample to be detected, the second detection channel carries out blood separation and mixing operation and detection operation on the current sample to be detected in sequence.

Step 163: and carrying out routine blood detection on the current sample to be detected in the fifth detection channel while carrying out detection operation on the second detection channel.

Step 164: and when the fifth detection channel is used for carrying out blood routine detection, the blood sampling and sample dividing assembly is controlled to collect the next detection sample, and the third detection channel is used for carrying out blood dividing and mixing operation and detection operation on the next sample to be detected.

Step 165: and when the third detection channel performs detection operation, the fourth detection channel sequentially performs blood separation and mixing operation and detection operation on the next sample to be detected.

After the blood separation and mixing operation in the routine blood detection of the fifth detection channel is completed, sequentially performing sample suction operation, blood separation and mixing operation and detection operation on a next sample to be detected in the third detection channel;

referring to fig. 17, fig. 17 is a schematic timing diagram of a fifth embodiment of a blood testing method according to the present application, where the timing may be specifically as follows:

3. After the blood separation and mixing operation of the first detection channel is completed, the sampling needle moves to the second detection channel, the blood sample is distributed to the second detection channel, and after the reagent is added, the power device carries out the mixing operation on the mixed solution of the reagent and the blood sample in the second detection channel; simultaneously, a first detection item (such as CRP) of the first detection channel starts detection, and the two operations are parallel;

5. After the blood routine detection detects a certain process, starting the sample suction of the second sample, and realizing the simultaneous sample suction of the second sample and the blood routine detection of the first sample through the arrangement of time sequences, thereby ensuring the realization of the detection speed of the preset sample;

6. The sampling and blood separating assembly moves to the upper part of the third detection channel, a blood sample of the second sample is separated into the third detection channel, and after a hemolytic agent and an antibody reagent for SAA are added, a power device is used for uniformly mixing the reagent in the third detection channel and the mixed solution of the blood sample;

7. After the third detection channel is evenly mixed, the sampling blood-separating assembly moves to the upper part of the fourth detection channel, the blood sample of the second sample is separated into the fourth detection channel, after the hemolytic agent and the antibody reagent for CRP are added, the power device is used for evenly mixing the reagent in the third detection channel and the mixed solution of the blood sample, meanwhile, the SAA project of the third detection channel is used for detecting, and the two operations are parallel;

8. After the blood separation and mixing action of the fourth detection channel is finished, the sampling blood separation assembly moves to the position above the fifth detection channel, the blood separation is carried out on the fifth detection channel so as to carry out conventional blood detection, meanwhile, CRP items of the fourth detection channel are detected, and the two operations are parallel;

9. After the blood routine detects a certain process, starting the sample suction of the third sample, and ensuring that the subsequent action is finished smoothly;

Compared with the prior art, in the embodiment, the blood routine detection and the multiple specific protein detection are respectively carried out on the multiple blood samples to be detected through the multiple channels, so that the blood routine detection and the multiple specific protein detection can be carried out on one device, the detection efficiency is improved, the trouble to a patient caused by multiple sampling is avoided, and on the other hand, the multiple detection steps can be simultaneously carried out in a time sequence superposition mode, so that the detection speed is further accelerated.

Referring to fig. 18, fig. 18 is a schematic structural diagram of an embodiment of a computer storage medium 180 provided in the present application, in which program data 181 is stored, which when executed by a processor, is configured to implement the following method:

Collecting a blood sample to be detected; controlling a plurality of detection channels to simultaneously and respectively detect blood routine and/or specific proteins of the blood sample to be detected; wherein the plurality of detection channels includes a blood routine detection channel and at least two specific protein detection channels, and the first detection step and the second detection step are controlled to be performed simultaneously when an operational component required for a first detection step being performed or to be performed by one of the plurality of detection channels is different from an operational component required for a second detection step to be performed by another detection channel.

Optionally, the processor is further configured to perform: controlling the sampling blood separating component to perform blood separating operation on the plurality of specific protein detection channels, and controlling the plurality of specific protein detection channels to perform reagent adding and mixing operation, detection operation and cleaning operation respectively; and controlling the sampling blood separating component to perform blood separating operation on the blood conventional detection channel, and controlling the blood conventional detection channel to perform reagent adding and mixing operation, detection operation and cleaning operation.

Optionally, the processor is further configured to perform: controlling the sampling blood separating component to move to a mixing pool of a target specific protein detection channel for blood separating operation; controlling a first power device to add reagent to a mixing pool of a target specific protein detection channel for mixing, and simultaneously controlling a sampling blood separation assembly to move to a mixing pool of a next specific protein detection channel for blood separation; the first power device is controlled to suck the blood sample to be detected which is uniformly mixed in the mixing pool of the target specific protein detection channel into the detection component of the target specific protein detection channel, and the second power device is controlled to uniformly mix the reagent in the mixing pool of the next specific protein detection channel; controlling a detection component of the target specific protein detection channel to detect the sucked blood sample to be detected, and simultaneously controlling a second power device to suck the blood sample to be detected which is uniformly mixed in a mixing pool of the next specific protein channel into the detection component of the next specific protein detection channel; controlling the cleaning component to clean the mixing pool of the target specific protein detection channel, and simultaneously controlling the detection component of the next specific protein detection channel to detect the sucked blood sample to be detected; and (3) cleaning the mixing pool of the next specific protein detection channel.

Optionally, the processor is further configured to perform: controlling a sampling and separating assembly to sample a blood sample to be detected; and controlling the sampling blood separating component to move to the vicinity of the liquid outlet end of the swab component, and performing blood throwing operation in the swab.

Optionally, the processor is further configured to perform: controlling the sampling blood separating component to move to a mixing pool of a target specific protein detection channel for blood separating operation; controlling the power device to add reagent to the mixing pool of the target specific protein detection channel for mixing, after mixing, controlling the power device to pump the mixed sample into the detection assembly, and simultaneously controlling the sampling blood separation assembly to move to the mixing pool of the next specific protein detection channel for blood separation; the detection assembly is controlled to detect a sample to be detected, the power device is controlled to carry out reagent adding and mixing operation on a mixing pool of a next specific protein detection channel, and after mixing is completed, the power device is controlled to pump the well mixed sample into the detection assembly; controlling the cleaning component to clean the mixing pool of the target specific protein detection channel, and simultaneously controlling the detection component of the next specific protein detection channel to detect the sucked blood sample to be detected; and (3) cleaning the mixing pool of the next specific protein detection channel.

Optionally, the processor is further configured to perform: controlling the sampling and blood separating component to move to the WBC detection channel to perform blood separating operation; after the to-be-detected blood sample in the WBC detection channel is diluted, controlling the sampling blood-separating component to perform a sample sucking operation on the to-be-detected blood sample in the WBC detection channel; controlling the power device to perform the first reagent adding and uniformly mixing operation on the WBC detection channel, and simultaneously controlling the sampling blood separating component to move to the RBC detection channel for blood separating operation; controlling the power device to perform secondary reagent adding and mixing operation on the WBC detection channel, and simultaneously controlling the power device to perform reagent adding and mixing operation on the RBC detection channel; detecting operation is carried out on the WBC detection channel and the RBC detection channel simultaneously; and (3) cleaning the WBC detection channel and the RBC detection channel simultaneously.

Optionally, the processor is further configured to perform: when the last specific protein channel performs detection operation, controlling a sampling blood-separating component to perform sample sucking operation on a blood sample to be detected in the WBC detection channel; when the last specific protein channel is subjected to cleaning operation, the power device is controlled to perform the first reagent adding and mixing operation on the WBC detection channel, and meanwhile, the sampling and blood separating assembly is controlled to move to the RBC detection channel to perform blood separating operation.

Optionally, the processor is further configured to perform: and controlling the sampling blood separating component to move to a liquid outlet of the swab component, and performing blood throwing operation in the swab.

In the several embodiments provided in the present application, it should be understood that the disclosed method and apparatus may be implemented in other manners. For example, the above-described device embodiments are merely illustrative, e.g., the division of the modules or units is merely a logical functional division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed.

The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the embodiment.

In addition, each functional unit in each embodiment of the present application may be integrated in one processing unit, each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.

The foregoing description is only of embodiments of the present application, and is not intended to limit the scope of the application, and all equivalent structures or equivalent processes according to the present application and the accompanying drawings, or direct or indirect application in other related technical fields, are included in the scope of the present application.

Claims

1. A blood test apparatus, the blood test apparatus comprising:

A plurality of detection channels including a blood routine detection channel and at least two specific protein detection channels;

A sampling blood separation component;

The controller is connected with the plurality of detection channels and the sampling blood separating component and is used for sequentially carrying out blood separating operation, reagent adding and mixing operation, detection operation and cleaning operation on the plurality of specific protein detection channels and carrying out blood separating operation, reagent adding and mixing operation, detection operation and cleaning operation on the blood regular detection channels when carrying out blood regular detection and/or specific protein detection on the same blood sample to be detected, wherein the operation component required by a first detection step which is executed or to be executed in one detection channel among the plurality of detection channels is different from the operation component required by a second detection step which is to be executed in the other detection channel, and the first detection step and the second detection step are controlled to be executed simultaneously;

Before the first specific protein detection channel performs blood separation operation, controlling the sampling blood separation assembly to perform a first sample suction operation and a blood throwing operation in the swab;

The sampling blood separating component is controlled to move to a mixing pool of the next specific protein detection channel for blood separating operation while reagent adding and mixing operation is carried out on the non-last specific protein detection channel;

When the last specific protein channel performs detection operation, controlling the sampling blood-separating component to perform sample sucking operation on a blood sample to be detected in the WBC detection channel; when the last specific protein channel is subjected to cleaning operation, controlling the power device to perform first reagent adding and mixing operation on the WBC detection channel;

the WBC detection channel of the blood routine detection channel is controlled to move to the RBC detection channel of the blood routine detection channel for blood separation operation while the WBC detection channel of the blood routine detection channel is subjected to the first reagent adding and mixing operation.

2. The blood test apparatus according to claim 1, wherein,

The blood detection device further comprises a swab component connected with the controller, and the controller is further used for controlling the sampling blood separation component to move to the vicinity of the liquid outlet end of the swab component so as to perform blood throwing operation in the swab.

3. The blood test apparatus according to claim 1, wherein,

The blood detection device further comprises a power device, and each specific protein detection channel comprises a mixing pool, a detection assembly, a first pipeline, a second pipeline, a third pipeline and a fourth pipeline;

the liquid power device is used for carrying out reagent adding operation and uniform mixing operation;

the first pipeline is connected with the mixing tank and is used for discharging waste liquid;

The second pipeline is connected with the mixing tank and is used for adding a hemolysis reagent into the mixing tank through the hydrodynamic device;

the third pipeline is connected with the mixing tank and is used for adding antibody reagent into the mixing tank through the hydrodynamic device;

The fourth pipeline is used for connecting the mixing pool with the liquid power device and carrying out mixing operation through the liquid power device;

the detection component is used for detecting the evenly mixed reagent.

4. A blood test apparatus according to claim 3, wherein,

The blood detection device further comprises a heating device for heating at least one of the mixing tank, the detection assembly, the second pipeline, the third pipeline and the fourth pipeline.

5. The blood test apparatus according to claim 4, wherein,

The blood test apparatus further includes:

The preheating tank is connected to the second pipeline and used for preheating the hemolysis reagent;

And the fifth pipeline is connected with the fourth pipeline and is used for providing diluent for the mixing tank and the detection assembly through the hydrodynamic device.

6. The blood test apparatus according to claim 5, wherein,

The heating device comprises a plurality of heating components with different temperatures, and the heating components are used for independently controlling or combining temperature of the preheating pool, the mixing pool, the detection component, the second pipeline, the third pipeline, the fourth pipeline and/or the fifth pipeline.

7. The blood test apparatus according to claim 6, wherein,

The heating component is a heating film or a heating rod, the specific protein detection module further comprises a plurality of heat conducting matrixes for conducting heat to the preheating pool, the mixing pool, the detection component, the second pipeline, the third pipeline, the fourth pipeline and/or the fifth pipeline, the heat conducting matrixes are in a block shape or a cylinder shape, the heating film is attached to the heat conducting matrixes, or the heating rod is inserted into the heat conducting matrixes, and the second pipeline, the third pipeline, the fourth pipeline and/or the fifth pipeline are wound on the cylinder-shaped heat conducting matrixes.

8. The blood test apparatus according to claim 5, wherein,

The heating device comprises a diluent heating pool, and the diluent heating pool is connected to the fifth pipeline and is arranged in parallel with the hydrodynamic device for carrying out uniform mixing operation relative to the fourth pipeline.

9. The blood test apparatus according to claim 1, wherein,

The plurality of specific protein detection channels are used to detect at least two proteins in SAA, CRP, TRF, hs-CRP, PCT, and D-Dimer.

10. A blood testing method, wherein the method is applied to the blood testing device of any one of claims 1-9, the method comprising:

Sequentially performing blood separation operation, reagent adding and mixing operation, detection operation and cleaning operation on a plurality of specific protein detection channels; and

Carrying out blood separation operation, reagent adding and mixing operation, detection operation and cleaning operation on a blood routine detection channel;

wherein the plurality of detection channels comprises a blood routine detection channel and at least two specific protein detection channels, and when an operation component required by a first detection step which is being executed or to be executed by one detection channel in the plurality of detection channels is different from an operation component required by a second detection step which is to be executed by another detection channel, the first detection step and the second detection step are controlled to be executed simultaneously;

wherein, before the first specific protein detection channel performs the blood separation operation, the sampling blood separation assembly is controlled to perform the first sample suction operation and the blood throwing operation in the swab;

11. The method of claim 10, wherein the step of determining the position of the first electrode is performed,

The method comprises the steps of sequentially carrying out blood separation operation, reagent adding and mixing operation, detection operation and cleaning operation on a plurality of specific protein detection channels, and comprises the following steps:

controlling the sampling blood separating component to move to a mixing pool of a target specific protein detection channel for blood separating operation;

Controlling a first power device to add reagent to a mixing pool of the target specific protein detection channel for mixing, and simultaneously controlling a sampling blood separation assembly to move to a mixing pool of the next specific protein detection channel for blood separation;

The first power device is controlled to suck the blood sample to be detected which is uniformly mixed in the mixing pool of the target specific protein detection channel into the detection component of the target specific protein detection channel, and the second power device is controlled to uniformly mix the reagent in the mixing pool of the next specific protein detection channel;

Controlling a detection component of a target specific protein detection channel to detect the sucked blood sample to be detected, and simultaneously controlling the second power device to suck the blood sample to be detected after being uniformly mixed in a mixing pool of a next specific protein channel into the detection component of the next specific protein detection channel;

Controlling a cleaning component to clean the mixing pool of the target specific protein detection channel, and simultaneously controlling a detection component of the next specific protein detection channel to detect the sucked blood sample to be detected;

And (3) cleaning the mixing pool of the next specific protein detection channel.

12. The method of claim 11, wherein the step of determining the position of the probe is performed,

When the target specific protein detection channel is the first specific protein detection channel in the plurality of specific protein detection channels, the control sampling blood separation assembly moves to a mixing pool of the target specific protein detection channels, and before the blood separation operation, the control sampling blood separation assembly further comprises:

controlling a sampling and separating assembly to sample a blood sample to be detected;

and controlling the sampling blood separating component to move to the vicinity of the liquid outlet end of the swab component, and performing blood throwing operation in the swab.

13. The method of claim 10, wherein the step of determining the position of the first electrode is performed,

Controlling a power device to add reagent to a mixing pool of the target specific protein detection channel for mixing, after mixing, controlling the power device to pump a well mixed sample into a detection assembly, and simultaneously controlling a sampling and blood separating assembly to move to a mixing pool of a next specific protein detection channel for blood separating operation;

The detection assembly is controlled to detect a sample to be detected, the power device is controlled to carry out reagent adding and mixing operation on a mixing pool of a next specific protein detection channel, and after mixing is completed, the power device is controlled to pump the well mixed sample into the detection assembly;

14. The method of claim 10, wherein the step of determining the position of the first electrode is performed,

The blood separation operation, the reagent adding and uniformly mixing operation, the detection operation and the cleaning operation are carried out on the blood routine detection channel, and the method comprises the following steps:

Controlling the sampling and blood separating component to move to the WBC detection channel to perform blood separating operation;

After the to-be-detected blood sample in the WBC detection channel is diluted, controlling a sampling blood-separating component to perform a sample sucking operation on the to-be-detected blood sample in the WBC detection channel;

controlling a power device to perform a first reagent adding and uniformly mixing operation on the WBC detection channel, and simultaneously controlling a sampling and blood separating component to move to the RBC detection channel for blood separating operation;

Controlling the power device to perform secondary reagent adding and mixing operation on the WBC detection channel, and simultaneously controlling the power device to perform reagent adding and mixing operation on the RBC detection channel;

performing detection operation on the WBC detection channel and the RBC detection channel simultaneously;

And simultaneously performing a cleaning operation on the WBC detection channel and the RBC detection channel.

15. The method of claim 14, wherein the step of providing the first information comprises,

The control power device carries out the first reagent adding and mixing operation on the WBC detection channel, and simultaneously controls the sampling blood separating component to move to the RBC detection channel for blood separating operation, and the control power device comprises:

when the last specific protein channel is subjected to cleaning operation, controlling the power device to perform the first reagent adding and uniformly mixing operation on the WBC detection channel, and simultaneously controlling the sampling blood separating component to move to the RBC detection channel for blood separating operation.

16. A computer storage medium, characterized in that the computer storage medium has stored therein program data for implementing the method according to any of claims 10-15 when being executed by a controller.