CN106124782A - Multiplex disposable blood transfusion cross match blood test combiner - Google Patents

Multiplex disposable blood transfusion cross match blood test combiner Download PDF

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Publication number
CN106124782A
CN106124782A CN201610528153.2A CN201610528153A CN106124782A CN 106124782 A CN106124782 A CN 106124782A CN 201610528153 A CN201610528153 A CN 201610528153A CN 106124782 A CN106124782 A CN 106124782A
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pipe
tube body
test
combiner
blood
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张禾云
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a kind of multiplex disposable blood transfusion cross match blood test combiner, including bracing frame, composite test pipe and the sampling injector of Aspirate supernatant, described composite test pipe includes upper tube body and lower tube body, described upper tube body includes straight pipe, described straight pipe uniformly connects along perimembranous at least four sample-adding pipe, the Basolateral of described straight pipe is provided with connecting portion outwardly, described lower tube body includes developmental tube, the sidepiece of described developmental tube is provided with the liquid suction pipe coordinated with sampling injector, connection auxiliary section outwardly it is provided with outside the upper end of described developmental tube.Present configuration is simple, it is adaptable to cohesion amine medium method multi-fork one blood mathcing test, and composite test pipe is formed by connecting by upper tube body and lower tube body are detachable, has both simplified manufacturing process, reduces cost, is easy to again in test be centrifuged lower tube body operation.

Description

Multiplex disposable blood transfusion cross match blood test combiner
Technical field
The present invention relates to technical field of medical instrument, be specifically related to a kind of multiplex disposable blood transfusion cross match blood test combination Device.
Background technology
Can cross matching determine that the important evidence transfused blood, and both sides all not coagulations can be transfused blood.Erythrocyte by the people that donates blood Mixing with serum and the erythrocyte of recipient respectively with serum, observe with or without agglutination, this test is referred to as cross matching examination Test.Cross match blood test includes master trip and secondary test two kinds.The former tries with blood donor's red cell suspension with donee's serum Test to find whether donee's serum contains the antibody reacted with blood donor's erythrocyte, also known as directly coordinating or master cooperation; Whether the latter then is tested to find to have in blood donor's serum not conform to antibody with blood donor's serum and donee's erythrocyte, also known as Connect cooperation.
At present, cross match blood test has various ways, such as salt water law, zymotechnic blood matching, colloidal medium blood matching, anti-ball Protein method and polybrene method, wherein polybrene method is tried with the serology such as qualification and cross matching for the mensuration of incomplete antibody Testing and promote the use of the most rapidly, the method is easy, the time is short, result is obvious.
The Chinese invention patent of Patent No. 201510077271.1 discloses a kind of multiplex disposable blood transfusion cross matching Test group clutch, it has transparent plastic small test tube, is provided with two sample-adding pipes in the upper end of small test tube, in the upper end of small test tube also Being provided with reagent adding and sample tap, at the distance bottom surface 4-10mm of the bottom of small test tube, fork-join has a liquid suction pipe, and is furnished with one and takes Sample viewer, it has suction pipe, and the tail end of suction pipe connects a transparent plastic film set, connects after transparent plastic film set There is a manual gasbag.It is extremely complex loaded down with trivial details that this cross match blood test combiner overcomes employing common test tube operation, and easily The problem made mistakes.But the versatility of this cross match blood test combiner is poor, for one fork one match, y-bend one match and The test such as trident one match needs different cuvette construction, easily occurs that test tube deposits confusion, test tube under-reserve etc. in reality Problem.
Summary of the invention
It is an object of the invention to overcome the above-mentioned problems in the prior art, it is provided that a kind of multiplex disposable blood transfusion is handed over Fork blood mathcing test combiner, it is adaptable to cohesion amine medium method multi-fork one blood mathcing test.
For realizing above-mentioned technical purpose, reaching above-mentioned technique effect, the present invention is to be achieved through the following technical solutions:
A kind of multiplex disposable blood transfusion cross match blood test combiner, including bracing frame, composite test pipe and absorption supernatant The sampling injector of liquid, described composite test pipe includes that upper tube body and lower tube body, described upper tube body include straight pipe, described directly Siphunculus uniformly connects along perimembranous at least four sample-adding pipe, and the Basolateral of described straight pipe is provided with connecting portion outwardly, Described lower tube body includes that developmental tube, the sidepiece of described developmental tube are provided with the liquid suction pipe coordinated with sampling injector, described developmental tube Upper end outside be provided with connection auxiliary section outwardly.
Further, the bottom of support frame as described above is provided with upper tube body case and lower tube body case, support frame as described above Top offers the groove running through front side and rear side, is positioned on the support body above described groove and is evenly equipped with supported hole, is positioned at described Support body upper surface below groove is evenly equipped with support slot.
Further, the outside of described upper tube body case and lower tube body case is provided with handle.
Further, described supported hole and support slot match with developmental tube specification.
Further, the caliber of described straight pipe matches with the caliber of developmental tube.
Further, the lower end of described connecting portion is provided with card column, described connection auxiliary section offers draw-in groove, passes through card column Cooperation with draw-in groove makes that straight pipe and developmental tube are detachable to be connected.
The invention has the beneficial effects as follows:
1, simple in construction, it is adaptable to cohesion amine medium method multi-fork one blood mathcing test, composite test pipe is by upper tube body and down tube Body is detachable to be formed by connecting, and has both simplified manufacturing process, reduces cost, is easy to again in test be centrifuged lower tube body Operation.
2, the bottom of bracing frame is provided with upper tube body case and lower tube body case, overcomes and easily occurs in reality that test tube is deposited Put confusion, the problem of test tube under-reserve.
Certainly, the arbitrary product implementing the present invention it is not absolutely required to reach all the above advantage simultaneously.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of the embodiment of the present invention, embodiment will be described required use below Accompanying drawing is briefly described, it should be apparent that, the accompanying drawing in describing below is only some embodiments of the present invention, for ability From the point of view of the those of ordinary skill of territory, on the premise of not paying creative work, it is also possible to obtain the attached of other according to these accompanying drawings Figure.
Fig. 1 is the structural representation of multiplex disposable blood transfusion cross match blood test combiner described in prior art;
Fig. 2 is composite test pipe and sampling in multiplex disposable blood transfusion cross match blood test combiner described in prior art The connection diagram of syringe;
Fig. 3 is the STRUCTURE DECOMPOSITION of composite test pipe in multiplex disposable blood transfusion cross match blood test combiner of the present invention Schematic diagram;
Fig. 4 is the structural representation of bracing frame in multiplex disposable blood transfusion cross match blood test combiner of the present invention.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.Based on Embodiment in the present invention, those of ordinary skill in the art obtained under not making creative work premise all other Embodiment, broadly falls into the scope of protection of the invention.
Referring to shown in Fig. 1-4, the present invention is a kind of multiplex disposable blood transfusion cross match blood test combiner, including supporting The sampling injector 4 of frame 1, composite test pipe and Aspirate supernatant, composite test pipe includes upper tube body 2 and lower tube body 3, upper pipe Body 2 includes straight pipe 201, and straight pipe 201 uniformly connects along perimembranous has at least four to be loaded pipe 202, outside the bottom of straight pipe 201 Side is provided with connecting portion 203 outwardly, and lower tube body 3 includes that developmental tube 301, the sidepiece of developmental tube 301 are provided with and sample injection The liquid suction pipe 302 that device 4 coordinates, is provided with connection auxiliary section 303 outwardly outside the upper end of developmental tube 301.
Wherein, the bottom of bracing frame 1 is provided with upper tube body case 101 and lower tube body case 102, the top of bracing frame 1 Offer the groove 103 running through front side and rear side, be positioned on the support body above groove 103 and be evenly equipped with supported hole 104, be positioned at groove Support body upper surface below 103 is evenly equipped with support slot 105.
Wherein, the outside of upper tube body case 101 and lower tube body case 102 is provided with handle.
Wherein, supported hole 104 and support slot 105 match with developmental tube 301 specification.
Wherein, the caliber of straight pipe 201 matches with the caliber of developmental tube 302.
Wherein, the lower end of connecting portion 203 is provided with card column, connects and offers draw-in groove, by card column and draw-in groove on auxiliary section 303 Cooperation make that straight pipe and developmental tube are detachable to be connected.
Apply combiner of the present invention, be condensed amine medium method trident one blood mathcing test step as follows:
1, taking 16 upper tube bodies 2 to having combined and lower tube body 3, the array that is square inserts the support slot 105 on bracing frame 1 top In, the sample-adding pipe 202 of each upper tube body 2 posts identifier number;
2, donee being compiled is No. 0, and it is 1,2, No. 3 that three blood donors compile respectively;
3, utilizing the first sample injector, be sequentially added into 0-3 blood cell in first to fourth row composite test pipe, blood cell quantity is two Drip;Utilizing the second sample injector, be sequentially added into 0-3 serum in first to fourth row composite test pipe, Serum Number is two;
4, in each composite test pipe, low solion (LIM) 0.65ml is added, again at each small test tube after mix homogeneously The middle cohesion amine aqueous solution that adds, (Polybene) solution two, and mix homogeneously, separation combination developmental tube, remove upper tube body 2, protect Leave body 3;
5, the lower tube body 3 after separating is jumped a queue, and uses special centrifugal machine that lower tube body 3 is centrifuged operation, after being centrifuged manually Being shaken gently for lower tube body 3, range estimation erythrocyte is with or without coagulation, as then reformed without coagulation;
6, add suspending agent (Resuspending) two and be shaken gently for lower tube body 3 mixing observed result, if 60 In second, coagulation is scattered, and representative is to be caused rejection to assemble match result by Polybene cohesion amine to be harmonious;As coagulation is not scattered, then For the specific reaction of red cell antigens antibodies, match result does not corresponds;
7, can coagulate, such as reaction, the supernatant then used in sampling injector absorption lower tube body 3, make slide sample, with micro- Sem observation.
In the description of this specification, the description of reference term " embodiment ", " example ", " concrete example " etc. means The specific features that describes in conjunction with this embodiment or example, structure, material are lived feature and are contained at least one of the present invention and implement In example or example.In this manual, the schematic representation to above-mentioned term is not necessarily referring to identical embodiment or example. And, the specific features of description, structure, material or feature can be to close in any one or more embodiments or example Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.Preferred embodiment is the most detailed Describe all of details, be also not intended to the detailed description of the invention that this invention is only described.Obviously, according to the content of this specification, Can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is to preferably explain the present invention Principle and actual application so that skilled artisan can be best understood by and utilize the present invention.The present invention is only Limited by claims and four corner thereof and equivalent.

Claims (6)

1. a multiplex disposable blood transfusion cross match blood test combiner, including bracing frame (1), composite test pipe and absorption supernatant The sampling injector (4) of liquid, it is characterised in that: described composite test pipe includes upper tube body (2) and lower tube body (3), described on Body (2) include straight pipe (201), described straight pipe (201) uniformly connect along perimembranous have at least four sample-adding pipe (202), institute The Basolateral stating straight pipe (201) is provided with connecting portion (203) outwardly, and described lower tube body (3) includes developmental tube (301), the sidepiece of described developmental tube (301) is provided with the liquid suction pipe (302) coordinated with sampling injector (4), described developmental tube (301) connection auxiliary section (303) outwardly it is provided with outside upper end.
Multiplex disposable blood transfusion cross match blood test combiner the most according to claim 1, it is characterised in that: described support The bottom of frame (1) is provided with upper tube body case (101) and lower tube body case (102), and the top of support frame as described above (1) offers Run through the groove (103) of front side and rear side, be positioned on the support body of described groove (103) top and be evenly equipped with supported hole (104), be positioned at The support body upper surface of described groove (103) lower section is evenly equipped with support slot (105).
Multiplex disposable blood transfusion cross match blood test combiner the most according to claim 2, it is characterised in that: described upper pipe The outside of body case (101) and lower tube body case (102) is provided with handle.
Multiplex disposable blood transfusion cross match blood test combiner the most according to claim 2, it is characterised in that: described support Hole (104) and support slot (105) match with developmental tube (301) specification.
Multiplex disposable blood transfusion cross match blood test combiner the most according to claim 1, it is characterised in that: described straight-through The caliber of pipe (201) matches with the caliber of developmental tube (302).
Multiplex disposable blood transfusion cross match blood test combiner the most according to claim 1, it is characterised in that: described connection The lower end in portion (203) is provided with card column, and described connection auxiliary section (303) offers draw-in groove.
CN201610528153.2A 2016-07-05 2016-07-05 Multiplex disposable blood transfusion cross match blood test combiner Pending CN106124782A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108593942A (en) * 2018-04-17 2018-09-28 杭州电子科技大学 A kind of cross matching method
CN110095617A (en) * 2019-05-08 2019-08-06 白银市红十字中心血站 A kind of disposable blood transfusion cross matching experiment combiner

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US3778232A (en) * 1971-11-26 1973-12-11 J Mcmorrow Blood typing system
CN1068726A (en) * 1991-07-24 1993-02-10 李建库 Disposable plastic blood sampling test tube
CN2158121Y (en) * 1993-02-04 1994-03-02 张国旗 Multi-function combined testing jig
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CN204865885U (en) * 2015-07-16 2015-12-16 郭丽华 Easy to clean's medical test tube
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108593942A (en) * 2018-04-17 2018-09-28 杭州电子科技大学 A kind of cross matching method
CN108593942B (en) * 2018-04-17 2020-09-08 杭州电子科技大学 Cross blood matching method
CN110095617A (en) * 2019-05-08 2019-08-06 白银市红十字中心血站 A kind of disposable blood transfusion cross matching experiment combiner

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