CN211741313U

CN211741313U - Blood grouping kit

Info

Publication number: CN211741313U
Application number: CN202020280433.8U
Authority: CN
Inventors: 王彦平; 李立和
Original assignee: Tianjin Baodi Hospital
Current assignee: Tianjin Baodi Hospital
Priority date: 2020-03-09
Filing date: 2020-03-09
Publication date: 2020-10-23
Anticipated expiration: 2030-03-09

Abstract

The utility model relates to a blood grouping kit especially relates to an use immunological technique to carry out blood grouping kit, the utility model discloses a cuboid box body reaches and buckles disjunctor lid rather than a side, and be provided with paper reagent frame in the box body, there are the reagent jack in six districts on the reagent frame, oval jack, circular jack, square jack, the rhombus jack, like oval jack, big circular jack, the reagent jack holds positive design respectively, anti-design, the antibody examination, auxiliary reagent bottle, such design is with blood group positive design, anti-design, irregular antibody detection blood group reagent is put together, and constitute by different shape reagent jack and different colour reagent bottle, high durability and convenient use, the characteristics that are difficult for obscuring.

Description

Blood grouping kit

Technical Field

The utility model relates to a blood grouping kit especially relates to an use immunity technique to carry out blood grouping kit.

Background

According to the existence of A antigen and B antigen on the surface of erythrocyte membrane, dividing the ABO blood group of erythrocyte into 4 types of A type, B type, AB type and O type, the blood agglutination test means that the antibody and erythrocyte generate macroscopic agglutination or hemolytic reaction in the liquid medium, through the erythrocyte agglutination test, carry on the positive, negative typing, thus accurately identify ABO blood group, positive typing, it is to use anti-A and anti-B blood group typing reagent to determine whether there is corresponding A antigen or B antigen on the surface of erythrocyte; reverse sizing is carried out by A₁Type B and type B erythrocyte reagent for determining whether corresponding anti-A exists in serum₁Or anti-B antibody according to red blood cell of the subjectThe ABO blood type is judged according to the conditions of A, B cell expressed antigen and anti-A and anti-B antibodies in serum (or plasma), and common detection methods comprise a slide method, a test tube method, a microplate method, a microcolumn agglutination method and the like; the irregular antibody is blood group antibodies except anti-A and anti-B, about 0.3-2% of patient serum contains the irregular antibody, which may be caused by pregnancy, blood transfusion or immune material injection, the antibody screening test aims at finding out the clinically significant irregular antibody, O type red blood cells of the clinically significant blood group antigen are selected, the antibody screening test is carried out by adopting a saline medium method, a coacervation amine method, a saline anti-human globulin method or a microcolumn agglutination anti-human globulin method, whether the accidental antibody of the red blood cells exists in the detected serum is detected, and if the irregular antibody exists in the serum of the detected person, the irregular antibody and the screened red blood cells carrying the corresponding antigen have positive reaction.

Disclosure of Invention

The utility model discloses a carry out blood grouping, and provide one kind and can carry out the blood group and just stereotype, anti-stereotype, irregular antibody detection blood grouping kit, the utility model who designs like this has the advantage be: all put the reagent that uses in detect reagent box, be convenient for store, transportation, detection, easy operation, use manpower sparingly and time, improved work efficiency.

The technical scheme is designed according to the following technical scheme:

a blood grouping kit comprises a cuboid box body and a box cover which is connected with one side edge of the cuboid box body in a bending mode, a paper reagent rack is arranged in the box body, reagent jacks which are divided into six regions, oval jacks, circular jacks, square jacks, rhombic jacks, similar oval jacks and large circular jacks are formed in the reagent rack, and the oval jacks respectively accommodate anti-A blood group shaping reagent bottles, anti-B blood group shaping reagent bottles and anti-D blood group shaping reagent bottles; the circular jacks respectively accommodate A₁The kit comprises an erythrocyte suspension reagent bottle, a B erythrocyte suspension reagent bottle and an O reagent erythrocyte suspension reagent bottle; the square jacks respectively accommodate a reagent bottle for screening red blood cells 1, a reagent bottle for screening red blood cells 2 and a reagent bottle for screening red blood cells 3; the diamond-shaped jacks respectively contain suspension reagent bottles for coagulation testA reagent bottle, a low-ion solution small reagent bottle; the large round jack is used for accommodating a large reagent bottle of low-ion solution; the similar oval jack holds A type quality control article reagent bottle, B type quality control article reagent bottle respectively, and such design is put together blood type positive design, anti-design, irregular antibody detection blood group identification reagent, comprises different shape reagent jack and different colour reagent bottle moreover, is difficult for obscuring.

Drawings

Fig. 1 is a schematic view of a three-dimensional structure of the reagent kit of the present invention.

In the figure: 1. box body 2. box cover

3. Reagent rack 4. oval jack

5. Round jack 6. square jack

7. Rhombic jack 8. oval jack

9. anti-A blood type shaping reagent bottle with large circular insertion hole 10

11. anti-B blood type sizing reagent bottle 12, anti-D blood type sizing reagent bottle

13.A₁Erythrocyte suspension reagent bottle 14.B erythrocyte suspension reagent bottle

Reagent bottle for O reagent and erythrocyte suspension 16 reagent bottle for screening erythrocyte 1

17. Screening erythrocyte 2 reagent bottle 18 screening erythrocyte 3 reagent bottle

19. Suspension reagent bottle 20. condensation reagent bottle

21. Small reagent bottle 22 of low ion solution and large reagent bottle of low ion solution

Type A quality control product reagent bottle 24 type B quality control product reagent bottle

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and examples.

The utility model provides a blood grouping kit, includes cuboid box body (1) and rather than a side bending disjunctor lid (2), and is provided with paper reagent frame (3) in the box body, is divided into oval jack (4) on the reagent frame, circular jack (5), square jack (6), rhombus jack (7), looks like oval jack (6)The blood group setting device comprises a circular insertion hole (8) and a large circular insertion hole (9), wherein the oval insertion hole (4) is used for accommodating an anti-A blood group setting reagent bottle (10), an anti-B blood group setting reagent bottle (11) and an anti-D blood group setting reagent bottle (12) respectively; the circular jacks (5) respectively contain A₁A red blood cell suspension reagent bottle (13), a B red blood cell suspension reagent bottle (14) and an O reagent red blood cell suspension reagent bottle (15); the square jacks (6) respectively accommodate a reagent bottle (16) for screening red blood cells 1, a reagent bottle (17) for screening red blood cells 2, a reagent bottle (18) for screening red blood cells 3, the diamond jacks (7) respectively accommodate a suspension reagent bottle (19), a coagulation reagent bottle (20) and a small low-ion solution reagent bottle (21); the large round jack (9) is used for accommodating a large reagent bottle (22) of low-ion solution; the similar oval-shaped jacks (8) are used for accommodating an A-type quality control reagent bottle (23) and a B-type quality control reagent bottle (24) respectively, the blood type is positively shaped, reversely shaped, irregular antibodies and auxiliary reagents are put together by the design, and the similar oval-shaped jacks and the reagent bottles with different colors are formed, so that the similar oval-shaped jacks are not easy to mix.

Example 1

AB0 blood group positive typing test

1. Taking three test tubes, marking, and respectively adding 1 drop of anti-A, anti-B and anti-D blood typing reagents into the test tubes.

2. Adding 1 drop of 3% -5% suspension of red blood cells to be detected into the labeled test tubes respectively.

3. The contents of the tube were gently mixed and centrifuged at 1000g for 15 s.

4. Taking out the test tube from the centrifuge, holding the test tube at an acute angle, slowly inclining to repeatedly wash the cell button with liquid, continuously slowly inclining and shaking when the red blood cells are not attached to the wall of the test tube any more, and observing whether a uniform red blood cell suspension or an agglutination is formed in the test tube.

5. Microscopic observations of suspected weak agglutination were used, and the observed agglutination intensity was recorded and the positive typing results were further verified by ABO reverse typing tests.

When the AB0 blood type positive typing test is performed every day, the A type quality control substance and the B type quality control substance should be simultaneously used for the control test, and the AB0 blood type positive typing test can be performed if the test is performed on the control.

Example 2

AB0 blood group reverse typing test

1. Three tubes were labeled, and 2 drops of serum (plasma) were added to each tube.

2. Adding 1 drop of 3% -5% A into the labeled test tube respectively₁B, O reagent for erythrocyte suspension.

3. The contents of the tube were gently mixed and centrifuged at 1000g for 15s in a hemoserology specific centrifuge.

4. Taking out the test tube from the centrifuge, holding the test tube at an acute angle, slowly inclining to repeatedly wash the red blood cell button with liquid, continuously slowly inclining and shaking when the red blood cells are not attached to the wall of the test tube any more, and observing whether a uniform red blood cell suspension or an agglutination is formed in the test tube.

5. Suspected of weak agglutination, the sample should be transferred to a microscope for observation, and the observed agglutination intensity recorded for mutual verification with the positive typing results.

Example 3

Irregular antibody assay

1. Taking 4 clean test tubes, marking 1, 2, 3 and self-contrast marks respectively.

2. Adding 2 drops of patient plasma (or serum) into each test tube, and adding 1 drop of corresponding 3% -5% reagent for screening red blood cells 1, screening red blood cells 2 and screening red blood cells 3 respectively, and mixing uniformly.

3. 0.65mL of low ionic medium solution (LIM) is added into each tube and mixed evenly (if the using amount is large, a large reagent bottle of the low ionic medium solution can be used).

4. Adding 2 drops of the coacervation amine solution into each tube, mixing uniformly, centrifuging for 15s at 1000g, discarding the supernatant without draining, leaving about 0.1mL of liquid at the bottom of the tube, slightly shaking the tube, observing whether agglutination exists or not by naked eyes, and performing the next test if agglutination exists, wherein the test can be repeated after the reason is searched if the reagent fails or the centrifugation condition does not meet the requirement.

5. 2 drops of suspension were added to each tube.

6. Mixing, and observing whether agglutination disappears within 1min, with or without hemolysis.

7. When weak agglutination reaction is suspected, 1 piece of clean glass slide is taken, 1 drop of erythrocyte suspension is absorbed from a suspected weak agglutination reaction tube by a disposable plastic dropper, the erythrocyte suspension is dropped on the glass slide and coated into a thin layer, the result is observed under a microscope, and the result is recorded.

Blood group experiment is including blood group just stereotyped, anti-experiment such as stereotyping, irregular antibody detection, including 15 kinds of reagent bottles, the utility model discloses combine this 15 reagents, form blood group appraisal kit, in order to avoid getting wrong reagent and cause the mistake, every type reagent bottle and jack diverse, different reagent bottles of same type experiment can take different colours reagent bottle to distinguish (different colours are not annotated to this patent), are convenient for like this store, transport, detect, easy operation, use manpower sparingly and time, have improved work efficiency.

Claims

1. A blood grouping kit characterized in that: cuboid box body (1) and rather than a side bend disjunctor lid (2), and be provided with paper reagent frame (3) in the box body, hold oval jack (4), circular jack (5), square jack (6), rhombus jack (7), similar oval jack (8), big circular jack (9) on the reagent frame, hold blood type positive design, anti-design, irregular antibody, auxiliary reagent bottle respectively.

2. The blood grouping kit according to claim 1, wherein the reagent rack (3) is a rectangular parallelepiped, and the oval insertion holes (4) of the reagent rack (3) are respectively used for accommodating the anti-A blood grouping reagent bottles (10), the anti-B blood grouping reagent bottles (11) and the anti-D blood grouping reagent bottles (12).

3. The kit according to claim 1, characterized in that the circular holes (5) of the rack (3) are adapted to receive A's, respectively₁An erythrocyte suspension reagent bottle (13), a B erythrocyte suspension reagent bottle (14) and an O reagent erythrocyte suspension reagent bottle (15).

4. The blood grouping kit according to claim 1, wherein the upper receptacles (6) of the reagent rack (3) are respectively adapted to receive a reagent bottle (16) for screening red blood cells 1, a reagent bottle (17) for screening red blood cells 2, and a reagent bottle (18) for screening red blood cells 3.

5. The blood grouping kit according to claim 1, wherein the rhombic shaped receptacles (7) of the reagent rack (3) are respectively adapted to receive a suspension reagent bottle (19), a coagulation reagent bottle (20) and a low ionic solution reagent bottle (21).

6. The blood grouping kit according to claim 1, wherein the large circular insertion holes (9) of the reagent rack (3) accommodate large reagent bottles (22) of low ionic solution.

7. The blood grouping kit according to claim 1, wherein the oval-like insertion holes (8) of the reagent rack (3) are respectively used for accommodating a type A quality control reagent bottle (23) and a type B quality control reagent bottle (24).

8. The blood grouping kit of claim 1 wherein the kit and the reagent support are paper or plastic.