CN214439214U

CN214439214U - ELISA assay enzyme-labeled coating plate placing device

Info

Publication number: CN214439214U
Application number: CN202021865463.1U
Authority: CN
Inventors: 邓银; 张蓉; 辜勋童; 黄健秋
Original assignee: Chengdu Zhongchuang Qingke Medical Diagnosis Test Center Co ltd
Current assignee: CHENGDU QINGKE BIO-TECHNOLOGY CO LTD
Priority date: 2020-08-31
Filing date: 2020-08-31
Publication date: 2021-10-22
Anticipated expiration: 2030-08-31

Abstract

The utility model discloses a placing device for enzyme-linked coating plate in ELISA enzyme-linked immunoassay experiment, which comprises a device body for supporting a placing plate of a kit; the device body is a cuboid, and the top of the cuboid is open; the device body is made of transparent plastic, and a device bottom plate is arranged at the bottom of the device body; the lower surface of the device bottom plate is provided with a transparent card slot; the area of the bottom of the card slot is the same as that of the bottom plate of the device; three sides of the card slot are bonded and fixed on the bottom plate of the device, and the other side of the card slot is provided with an inlet of the card slot; an arc-shaped opening is formed in the card slot at the inlet of the card slot, a cover cap matched with the arc-shaped opening is arranged at the edge end of the inlet of the card slot, and a buckle matched with the clamping groove is arranged on the cover cap; the card slot is used for accommodating paper, and the paper is rectangular; a plurality of unit grids are uniformly distributed on the rectangular paper, and a round hole is drawn in each unit grid; and the round holes are marked with different colors.

Description

ELISA assay enzyme-labeled coating plate placing device

Technical Field

The utility model belongs to the technical field of Elisa experimental apparatus, concretely relates to ELISA enzyme-linked immunosorbent assay experiment ELIAS wrapper sheet placer.

Background

The Elisa test is an experimental method with high sensitivity, strong specificity and good repeatability. Because of the factors of stable reagent, easy storage, simple operation, objective result judgment and the like, the method is widely applied to various fields of immunological test. The ELISA detection kit is suitable for qualitatively detecting the activity or content of various enzymes in human serum or plasma in vitro. The basis of ELISA is immobilization of antigen or antibody and enzyme labeling of antigen or antibody. The antigen or antibody combined on the surface of the solid phase carrier still keeps the immunological activity, and the enzyme-labeled antigen or antibody keeps the immunological activity and the enzyme activity. In the measurement, the specimen to be tested (the antibody or antigen to be measured therein) is reacted with the antigen or antibody on the surface of the solid carrier. The antigen-antibody complex formed on the solid phase carrier is separated from other substances in the liquid by washing. Then, an enzyme-labeled antigen or antibody is added thereto, and the resulting mixture is bound to a solid-phase carrier by reaction. The amount of enzyme on the solid phase is in a certain ratio to the amount of the substance to be detected in the sample. After the substrate of enzyme reaction is added, the substrate is catalyzed by enzyme to become a colored product, and the amount of the product is directly related to the amount of the detected substance in the sample, so that qualitative or quantitative analysis can be carried out according to the color depth. The catalytic efficiency of the enzyme is high, so that the result of immune reaction is indirectly amplified, and the determination method achieves high sensitivity. ELISA can be used to measure antigens as well as antibodies.

During the experiment, the different proteins or enzymes can be assayed by replacing the enzyme-labeled coated plate. Add 96 hole experiment reagents, because the hole number is more, the different liquid that adds all is transparent colour, can appear the phenomenon of unclear sign when carrying out the application of sample of different materials and survey, forgets or has confused which hole should add which kind of reagent, and it adds or wrong liquid is easily added.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to the above-mentioned among the prior art not enough, provide an ELISA enzyme-linked immunosorbent assay experiment ELIAS clad plate placer to solve and can appear the unclear phenomenon of sign when carrying out the application of sample of different materials survey, forget or obscure which hole should add which kind of reagent, add the problem of perhaps wrong liquid of adding by mistake easily.

In order to achieve the purpose, the utility model adopts the technical proposal that:

an ELISA (enzyme-linked immunosorbent assay) coated plate placing device for an ELISA comprises a device body for supporting a kit placing plate; the device body is a cuboid, and the top of the cuboid is open; the device body is made of transparent plastic, and a device bottom plate is arranged at the bottom of the device body; the lower surface of the device bottom plate is provided with a transparent card slot; the area of the bottom of the card slot is the same as that of the bottom plate of the device; three sides of the card slot are bonded and fixed on the bottom plate of the device, and the other side of the card slot is provided with an inlet of the card slot; an arc-shaped opening is formed in the card slot at the inlet of the card slot, a cover cap matched with the arc-shaped opening is arranged at the edge end of the inlet of the card slot, and a buckle matched with the clamping groove is arranged on the cover cap;

the card slot is used for accommodating paper, and the paper is rectangular; a plurality of unit grids are uniformly distributed on the rectangular paper, and a round hole is drawn in each unit grid; and the round holes are marked with different colors.

Preferably, the device body is 0.1cm thick, 12.5cm long, 8.5cm wide and 1.5cm high.

Preferably, the paper is uniformly distributed with 8 rows and 12 columns, and 96 round holes in total.

Preferably, the paper is 12.2cm long and 8.1cm wide.

Preferably, the distance between the centers of two adjacent round holes is 1 cm.

Preferably, the distance between the center point of the circular hole in the leftmost row and the left edge of the paper is 1.5cm, and the distance between the center point of the circular hole in the first row and the upper edge of the paper is 1.1 cm.

The utility model provides a ELISA enzyme-linked immunosorbent assay peridium board placer has following beneficial effect:

the utility model is used for use with the kit from the board cooperation of placing of taking, carry out the bearing to placing the board to can survey different protein or enzyme on a board simultaneously, only need transform through the colour with card inslot paper round hole, and then can distinguish standard hole, blank empty and the proof test hole of the material of different grade type, can carry out the application of sample more fast, and be difficult for makeing mistakes.

The utility model discloses when carrying out the survey experiment of multiple type material in the experiment system, can be at will decide certain round hole for appointed type, for example green represents the standard substance of first kind of material, red represents the test article of first kind of material, blue represents the standard substance of second kind of material, yellow represents the test article of second kind of material, can add different reagents according to the colour of difference like this to reduce the mistake that the application of sample in-process caused, thereby avoid causing the waste of experiment system.

Drawings

FIG. 1 is a schematic structural diagram of an ELISA plate holder for ELISA.

FIG. 2 is a top view of the ELISA plate holder for ELISA.

FIG. 3 is a diagram of the structure of the paper sheet of the ELISA plate holder for ELISA.

Wherein, 1, the device body; 2. a device chassis; 3. a card slot; 4. a card slot entrance; 5. an arc-shaped opening; 6. capping; 7. a card slot; 8. buckling; 9. paper; 10. a unit square; 11. a circular hole.

Detailed Description

The following description of the embodiments of the present invention is provided to facilitate the understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and various changes may be made apparent to those skilled in the art within the spirit and scope of the present invention as defined and defined by the appended claims.

According to an embodiment of the application, referring to fig. 1, the ELISA plate placing device for ELISA experiments in the present scheme includes a device body 1, which is used in cooperation with a placing plate of a kit itself to support the placing plate.

Device body 1 is the cuboid, and the open-top of cuboid can be with placing the board embedding wherein, and the material of device body 1 is transparent plastic, is convenient for observe.

The thickness of the device body 1 is 0.1cm, the length is 12.5cm, the width is 8.5cm, and the height is 1.5 cm. The size design of the device body 1 is consistent with the size of a placing plate of the kit, and the device body can be better combined with the placing plate for use in a matched mode.

The bottom of the device body 1 is provided with a device bottom plate 2; the lower surface of the device bottom plate 2 is provided with a transparent card slot 3; the card slot 3 has the same slot floor area as the device floor 2.

Three edges of the card slot 3 are bonded and fixed on the device bottom plate 2, and the bonding width is 0.3 cm; the remaining side is not bonded to the bottom surface, and is provided with a card slot entrance 4, and the paper 9 is put in and taken out from the direction of the card slot entrance.

An arc-shaped opening 5 is formed in the card slot 3 at the card slot inlet 4, and the arc-shaped opening 5 facilitates drawing check, namely putting in and drawing out, of the paper 9.

And 4 limit ends at the card slot entry set up with arc opening 5 matched with block 6, set up on block 6 with draw-in groove 7 complex buckle 8.

The buckle 8 is clamped into the clamping groove 7, and the cover cap 6 is covered on the arc-shaped opening 5 and used for protecting the paper 9 from being polluted by the reagent.

The card slot 3 is used for accommodating paper 9, and the paper 9 is rectangular; a plurality of unit squares 10 are uniformly distributed on the rectangular paper 9, and a round hole 11 is drawn in each unit square 10; and marks with different colors are marked on a plurality of the round holes 11.

8 rows and 12 columns of 96 round holes 11 are uniformly distributed on the paper 9, and the length of the paper 9 is 12.2cm and the width of the paper 9 is 8.1 cm.

The distance between the centers of two adjacent round holes 11 is 1 cm; the distance between the center point of the circular hole 11 in the leftmost row and the left edge of the paper 9 is 1.5cm, and the distance between the center point of the circular hole 11 in the first row and the upper edge of the paper 9 is 1.1 cm.

The utility model discloses a work flow does:

before the experiment, drawing paper 9 according to actual conditions earlier, showing different reagent with different colours and putting the region, inserting paper 9 in card groove 3, the one side of taking the colour is towards the upper portion of square, convenient observation. And putting the placing plate into the sample adding device, and combining the placing plate and the sample adding device to form a complete sample adding device system for sample adding. The loading was completed and the placement device was removed prior to optical verification by the microplate reader, and the absorbance (OD value) was measured.

The utility model is used for use with the kit from the board cooperation of placing in area, carry out the bearing to placing the board to can survey different protein or enzyme on a board simultaneously, only need transform through the colour with 9 round holes 11 of paper in the card groove 3, and then can distinguish standard hole, blank empty and the proof hole of the material of different grade type, can carry out the application of sample more fast, and be difficult for makeing mistakes.

The utility model discloses when carrying out the survey experiment of multiple type material in the experiment system, can be at will with certain round hole 11 decide for appointed type, for example green represents the standard substance of first kind of material, red represents the test article of first kind of material, blue represents the standard substance of second kind of material, yellow represents the test article of second kind of material, can add different reagents according to the colour of difference like this to reduce the mistake that the application of sample in-process caused, thereby avoid causing the waste of experiment system.

While the present invention has been described in detail with reference to the embodiments, the scope of the present invention should not be limited to the embodiments. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.

Claims

1. An ELISA enzyme-labeled coating plate placing device for ELISA enzyme-linked immunoassay experiments is characterized in that: comprises a device body for supporting a reagent box placing plate; the device body is a cuboid, and the top of the cuboid is open; the device body is made of transparent plastic, and a device bottom plate is arranged at the bottom of the device body; the lower surface of the bottom plate of the device is provided with a transparent card slot; the area of the bottom of the card slot is the same as that of the bottom plate of the device; three sides of the card slot are bonded and fixed on the bottom plate of the device, and the other side of the card slot is provided with an inlet of the card slot; an arc-shaped opening is formed in the card slot at the inlet of the card slot, a cap matched with the arc-shaped opening is arranged at the edge end of the inlet of the card slot, and a buckle matched with the clamping groove is arranged on the cap;

2. The ELISA plate-coating placing device for ELISA assay of claim 1, characterized in that: the thickness of the device body is 0.1cm, the length is 12.5cm, the width is 8.5cm, and the height is 1.5 cm.

3. The ELISA plate-coating placing device for ELISA assay of claim 1, characterized in that: 8 rows and 12 columns of 96 round holes are uniformly distributed on the paper.

4. The ELISA plate-coating placing device for ELISA assay of claim 1, characterized in that: the paper was 12.2cm long and 8.1cm wide.

5. The ELISA plate-coating placing device for ELISA assay of claim 1, characterized in that: the distance between the centers of the two adjacent round holes is 1 cm.

6. The ELISA plate-coating placing device for ELISA assay of claim 1, characterized in that: the distance between the center point of the round hole in the leftmost row and the left edge of the paper is 1.5cm, and the distance between the center point of the round hole in the first row and the upper edge of the paper is 1.1 cm.